Starting /dee2/code/volunteer_pipeline.sh SRR13695421 current disk space = 3050878767104 free memory = 910753112 SRR13695421 SRAfilesize 6430122b9d11afd25942672c55381db7 SRR13695421.sra SRR13695421.sra file validated SRR13695421 is paired end SRR13695421 is conventional basespace SRR13695421 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695421_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5605 37.0 37.0 37.0 37.0 37.0 2 36.285 37.0 37.0 37.0 37.0 37.0 3 36.539 37.0 37.0 37.0 37.0 37.0 4 36.5095 37.0 37.0 37.0 37.0 37.0 5 36.55 37.0 37.0 37.0 37.0 37.0 6 36.5815 37.0 37.0 37.0 37.0 37.0 7 36.4835 37.0 37.0 37.0 37.0 37.0 8 36.5335 37.0 37.0 37.0 37.0 37.0 9 36.5045 37.0 37.0 37.0 37.0 37.0 10-14 36.5214 37.0 37.0 37.0 37.0 37.0 15-19 36.5201 37.0 37.0 37.0 37.0 37.0 20-24 36.4987 37.0 37.0 37.0 37.0 37.0 25-29 36.4134 37.0 37.0 37.0 37.0 37.0 30-34 36.383799999999994 37.0 37.0 37.0 37.0 37.0 35-39 36.4521 37.0 37.0 37.0 37.0 37.0 40-44 36.4055 37.0 37.0 37.0 37.0 37.0 45-49 36.315799999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.317099999999996 37.0 37.0 37.0 37.0 37.0 55-59 36.3622 37.0 37.0 37.0 37.0 37.0 60-64 36.2735 37.0 37.0 37.0 37.0 37.0 65-69 36.2508 37.0 37.0 37.0 37.0 37.0 70-74 36.2656 37.0 37.0 37.0 37.0 37.0 75-79 36.1998 37.0 37.0 37.0 37.0 37.0 80-84 36.1241 37.0 37.0 37.0 37.0 37.0 85-89 36.2059 37.0 37.0 37.0 37.0 37.0 90-94 36.0757 37.0 37.0 37.0 37.0 37.0 95-99 36.0732 37.0 37.0 37.0 37.0 37.0 100-104 36.0776 37.0 37.0 37.0 37.0 37.0 105-109 36.0341 37.0 37.0 37.0 37.0 37.0 110-114 36.051 37.0 37.0 37.0 37.0 37.0 115-119 36.0131 37.0 37.0 37.0 37.0 37.0 120-124 35.944399999999995 37.0 37.0 37.0 37.0 37.0 125-129 35.8713 37.0 37.0 37.0 37.0 37.0 130-134 35.9516 37.0 37.0 37.0 37.0 37.0 135-139 35.8337 37.0 37.0 37.0 37.0 37.0 140-144 35.7707 37.0 37.0 37.0 37.0 37.0 145-149 35.6708 37.0 37.0 37.0 37.0 37.0 150-151 35.39725 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 1.0 22 0.0 23 0.0 24 1.0 25 5.0 26 7.0 27 15.0 28 10.0 29 13.0 30 33.0 31 40.0 32 55.0 33 74.0 34 134.0 35 335.0 36 2933.0 37 343.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.3 12.775 8.075000000000001 34.849999999999994 2 20.15562248995984 13.50401606425703 35.24096385542169 31.099397590361445 3 17.4 18.725 27.700000000000003 36.175000000000004 4 21.85 27.6 22.825 27.725 5 24.224999999999998 30.075000000000003 23.150000000000002 22.55 6 19.15 35.825 24.15 20.875 7 15.7 28.925 39.95 15.425 8 18.175 24.9 31.574999999999996 25.35 9 17.275 25.775 32.05 24.9 10-14 19.68 30.005 27.32 22.994999999999997 15-19 20.26 27.800000000000004 28.345 23.595 20-24 20.69 29.134999999999998 27.175 23.0 25-29 20.200000000000003 29.4 27.275 23.125 30-34 20.39 28.76 27.875 22.975 35-39 20.595 28.355000000000004 27.794999999999998 23.255 40-44 19.66 29.755 27.505000000000003 23.080000000000002 45-49 20.345 28.945 27.284999999999997 23.425 50-54 19.825 29.439999999999998 27.185 23.549999999999997 55-59 20.47 28.4 27.77 23.36 60-64 20.005 28.415000000000003 27.73 23.849999999999998 65-69 20.495 28.125 27.04 24.34 70-74 19.78 28.804999999999996 27.905 23.51 75-79 20.49 27.839999999999996 27.825 23.845 80-84 20.52 27.675 28.095 23.71 85-89 20.294999999999998 28.155 28.444999999999997 23.105 90-94 20.43 28.785 27.169999999999998 23.615 95-99 20.785 28.37 27.36 23.485 100-104 20.105 28.825 27.185 23.885 105-109 21.205 28.449999999999996 26.755000000000003 23.59 110-114 21.375 27.860000000000003 27.08 23.685000000000002 115-119 20.925 27.939999999999998 27.029999999999998 24.104999999999997 120-124 21.425 27.465 27.54 23.57 125-129 21.275 28.28 26.77 23.674999999999997 130-134 21.2 28.17 26.979999999999997 23.65 135-139 20.630000000000003 28.050000000000004 27.85 23.47 140-144 21.92 28.265 26.345000000000002 23.47 145-149 21.725 28.87 26.025 23.380000000000003 150-151 21.8125 27.125 26.35 24.712500000000002 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.0 19 0.0 20 0.5 21 2.5 22 2.5 23 1.5 24 3.0 25 3.5 26 3.0 27 3.5 28 11.0 29 16.5 30 13.0 31 19.0 32 35.0 33 51.5 34 55.5 35 64.5 36 87.5 37 117.5 38 144.0 39 173.5 40 201.0 41 220.5 42 248.5 43 262.0 44 255.0 45 241.5 46 243.0 47 241.0 48 213.5 49 187.5 50 180.0 51 154.5 52 113.0 53 90.5 54 73.5 55 59.5 56 52.0 57 45.0 58 30.0 59 18.0 60 15.0 61 8.0 62 3.5 63 6.0 64 9.0 65 8.0 66 4.0 67 0.5 68 1.5 69 1.5 70 0.0 71 0.0 72 0.0 73 1.0 74 1.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.425 #Duplication Level Percentage of deduplicated Percentage of total 1 70.99013518450859 48.575 2 18.706613080014613 25.6 3 6.284252831567409 12.9 4 2.3383266350018266 6.4 5 0.8403361344537815 2.875 6 0.5845816587504566 2.4 7 0.21921812203142127 1.05 8 0.03653635367190354 0.2 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTGCTAACCTCACTACCAAGTCCGGACCTGGATCTAGAGAGCTTTTCGA 8 0.2 No Hit GGTCCATTCGAAAGATCAAAAGCTGCATCATTTAAATCTAATCTAAGATC 7 0.17500000000000002 No Hit GCTCCAAATACAAACCGGTAAAGTGGTCTTCAATATGGATTGAGGTTTCT 7 0.17500000000000002 No Hit CTCAGAATCATTCAGCTCTCCCTTGGAATGTTCAGTTAGAGTAAGGTTCT 7 0.17500000000000002 No Hit ATCACAGTGTAGTGGTTATTTACACAAGTTCCTTCAGGAAATATGAGAAG 7 0.17500000000000002 No Hit TTCTCAGTTGAATTCTTTAACATGATTATAAGAATTCGGACAAACACAGC 7 0.17500000000000002 No Hit ACTCGATCTGTGATTAAGAAACTTAATCAATATCTAAACTGTGAAAGCTA 7 0.17500000000000002 No Hit GCCAAACTTCTGGTCTAGGACCTTGACGAAGGGAGCAAGGCAGTTAGTGG 6 0.15 No Hit CCCAATCTACGAACTGCATACCACCATAAATATTGTTGGCAAATCGCACA 6 0.15 No Hit CCCGAACGGAGATCATAGTGATGGTTCATCGCACAACAAAAGAGAAATTA 6 0.15 No Hit CTCTGATCCAAAGTCATTTGCAGTTGGAAAACGCCACCTTAAAAGGTTCA 6 0.15 No Hit CTTCTCTGGTGCCACACCAAGAACCAGAGTTTCGTTGGTGATCGTCTCTG 6 0.15 No Hit GGCCCATCACCAGAAAAGTAAGGTTTGATTGCTTCAATAAAAACATCACA 6 0.15 No Hit ACGGAGAGTAAAGGGCTGTTTGTTGGAGTTGGAGTAGGGGGAGAGTGAGA 6 0.15 No Hit GCTTCATAATTGATTTGTTGGCCCATGGAACTTAATTTGGTGAGAGTAGT 6 0.15 No Hit GGACTCTTATCTTCGACCCATAAATACAGCTACTTCTACGTTACAACGAT 6 0.15 No Hit GTTCCTTTTCTCTGTGGACGATTGGATCAGCTGACCAAATTGACTGGGAA 6 0.15 No Hit GGCGCTATCAACAAGCTTCAACTTAGCCAATAAGTCCACTGCTTCCACAA 6 0.15 No Hit GTGCAATTACAGGTAAATTTATGCCAATCACACCCCCAACAAAACCCATC 6 0.15 No Hit CAGTCATAGCTTTCTTTGAGTAGATACTCCTGTAAGTGAAGGAGATGTTG 6 0.15 No Hit CTCCAATCAAAATGACACCAAGATCCACAAGTCAATAAAACATAGCATGA 6 0.15 No Hit CCTGGTCAATCGCATCCTCAATTTTCTTCTTGTCATCTGCACCCAGCTTG 6 0.15 No Hit CACCCCTCCAAATACTCCACAAAACTCCTATCATCAGCAACAATCAACGT 6 0.15 No Hit ATGAAATGACGGCTGGCGGTGCTTGGAGAGCAAATAAAGGAACAAGAAAG 5 0.125 No Hit GCCCCTTGGATGCAAACTCTGACAAACTTGGCATGTAAGCATCTCATTCA 5 0.125 No Hit GTTCCTTGAGTCTGCTGAGGATATGAATTTCTGTAATGAGTCATTTGGCA 5 0.125 No Hit GCAAGCTTGTTTCACCCTGAAATTCATTAAGAAAGATTCCCGCAGCAAAT 5 0.125 No Hit GGCCAGGGCATGTAAATGTGCTTGTAGGGTCATCCTGGGGATAGCTATAA 5 0.125 No Hit TGTATTTTTTGGAAAATGCCTTGTGCGATGACGGATCATCACCACTAATT 5 0.125 No Hit GTCGTTAATATCTATTTGGATTTCTGCCTGCTTGGTTGACGAAACACCAA 5 0.125 No Hit CTCAGCTCCCCCGTCAAGGGTTGGGCACTGGTTAGCAGTTCCAGATCCTT 5 0.125 No Hit CCCATGGCTATAAAATCTTTTAAACATTTAGATAAATTAGTGTTGCAGTT 5 0.125 No Hit CCCAGAATCTCCATTGACTGGAAAAGAATTCATTGATCCAAACTGATTAA 5 0.125 No Hit TCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGC 5 0.125 No Hit TCTGGATTAGGGATATGATTTGGAAACATACCATCTGGTTCCAAGAACTG 5 0.125 No Hit CTCCTGTCAATGCCATGCTCACCATCAAGCAAGATTTCTGGCTCCACAAT 5 0.125 No Hit CTTTGTTTGTTCCGACCATATCCATCTTGTCAGAAATCTGAGGAGAATCA 5 0.125 No Hit GCTTCTTCCAAGAACTGCTTATACTCCTGCAAACGTTTTTTATCCGGATA 5 0.125 No Hit GTTGTCGAATCCGATTATACGGATAAAGGCGTTAGGGTAAGCTTTCTTTG 5 0.125 No Hit CTTGCCATCAGTGGTGGATTGGTAGAGAGTCTCCTCAAAAAGGATGGCAC 5 0.125 No Hit AAGAAGATGAACCAGCTCTGAGAAAAGTGGAGCAGCATCAGGTCTGACCA 5 0.125 No Hit GTTTCTGAAGCACTTTTCTTAATGGAGGTTATTGAAGTAGTAATTGTGTT 5 0.125 No Hit GGATTTACTCTGGTTAGAAGCTTATGTAAACAAAATGAAGCAAGGCAACA 5 0.125 No Hit ATCATCACTGTCTACTTGGTGGAACCTGTAGCAATTGGAGACAACAGTCA 5 0.125 No Hit CCACTATCTTGACTGCTTCCTTATTTTCAGTTTTTGTTCCTGCTTGTTTC 5 0.125 No Hit GACGAGTGTTGTCGAATCCAATGATACGGATAAAGGAGTTAGGGTAAGCT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.037500000000000006 0.0 0.0 0.0 0.0 76-77 0.0625 0.0 0.0 0.0 0.0 78-79 0.0875 0.0 0.0 0.0 0.0 80-81 0.125 0.0 0.0 0.0 0.0 82-83 0.16249999999999998 0.0 0.0 0.0 0.0 84-85 0.225 0.0 0.0 0.0 0.0 86-87 0.225 0.0 0.0 0.0 0.0 88-89 0.25 0.0 0.0 0.0 0.0 90-91 0.275 0.0 0.0 0.0 0.0 92-93 0.375 0.0 0.0 0.0 0.0 94-95 0.6625 0.0 0.0 0.0 0.0 96-97 0.725 0.0 0.0 0.0 0.0 98-99 0.7625 0.0 0.0 0.0 0.0 100-101 0.95 0.0 0.0 0.0 0.0 102-103 1.125 0.0 0.0 0.0 0.0 104-105 1.3625 0.0 0.0 0.0 0.0 106-107 1.6 0.0 0.0 0.0 0.0 108-109 1.9 0.0 0.0 0.0 0.0 110-111 2.025 0.0 0.0 0.0 0.0 112-113 2.3499999999999996 0.0 0.0 0.0 0.0 114-115 2.6875 0.0 0.0 0.0 0.0 116-117 3.025 0.0 0.0 0.0 0.0 118-119 3.5374999999999996 0.0 0.0 0.0 0.0 120-121 4.075 0.0 0.0 0.0 0.0 122-123 4.5625 0.0 0.0 0.0 0.0 124-125 5.199999999999999 0.0 0.0 0.0 0.0 126-127 5.5625 0.0 0.0 0.0 0.0 128-129 6.075 0.0 0.0 0.0 0.0 130-131 6.575 0.0 0.0 0.0 0.0 132-133 7.1875 0.0 0.0 0.0 0.0 134-135 7.987500000000001 0.0 0.0 0.0 0.0 136-137 8.425 0.0 0.0 0.0 0.0 138-139 9.0125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAACTCC 30 0.0017973486 72.5 145 >>END_MODULE SRR13695421 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695421_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.96975 37.0 37.0 37.0 37.0 37.0 2 36.01 37.0 37.0 37.0 37.0 37.0 3 36.102 37.0 37.0 37.0 37.0 37.0 4 36.101 37.0 37.0 37.0 37.0 37.0 5 36.139 37.0 37.0 37.0 37.0 37.0 6 36.2905 37.0 37.0 37.0 37.0 37.0 7 36.1415 37.0 37.0 37.0 37.0 37.0 8 36.157 37.0 37.0 37.0 37.0 37.0 9 36.2635 37.0 37.0 37.0 37.0 37.0 10-14 36.2301 37.0 37.0 37.0 37.0 37.0 15-19 36.0961 37.0 37.0 37.0 37.0 37.0 20-24 36.0753 37.0 37.0 37.0 37.0 37.0 25-29 36.0594 37.0 37.0 37.0 37.0 37.0 30-34 35.9583 37.0 37.0 37.0 37.0 37.0 35-39 35.961499999999994 37.0 37.0 37.0 37.0 37.0 40-44 35.9659 37.0 37.0 37.0 37.0 37.0 45-49 35.989749999999994 37.0 37.0 37.0 37.0 37.0 50-54 35.92380000000001 37.0 37.0 37.0 37.0 37.0 55-59 35.86749999999999 37.0 37.0 37.0 37.0 37.0 60-64 35.8497 37.0 37.0 37.0 37.0 37.0 65-69 35.8448 37.0 37.0 37.0 37.0 37.0 70-74 35.7673 37.0 37.0 37.0 37.0 37.0 75-79 35.82795 37.0 37.0 37.0 37.0 37.0 80-84 35.86024999999999 37.0 37.0 37.0 37.0 37.0 85-89 35.6717 37.0 37.0 37.0 37.0 37.0 90-94 35.67265 37.0 37.0 37.0 37.0 37.0 95-99 35.75189999999999 37.0 37.0 37.0 37.0 37.0 100-104 35.71040000000001 37.0 37.0 37.0 37.0 37.0 105-109 35.6991 37.0 37.0 37.0 37.0 37.0 110-114 35.599599999999995 37.0 37.0 37.0 37.0 37.0 115-119 35.553200000000004 37.0 37.0 37.0 37.0 37.0 120-124 35.4512 37.0 37.0 37.0 37.0 37.0 125-129 35.521750000000004 37.0 37.0 37.0 37.0 37.0 130-134 35.3817 37.0 37.0 37.0 37.0 37.0 135-139 35.324799999999996 37.0 37.0 37.0 34.6 37.0 140-144 35.24470000000001 37.0 37.0 37.0 34.6 37.0 145-149 35.1281 37.0 37.0 37.0 27.4 37.0 150-151 34.854875 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 4.0 14 10.0 15 2.0 16 2.0 17 1.0 18 0.0 19 0.0 20 0.0 21 4.0 22 3.0 23 9.0 24 4.0 25 10.0 26 18.0 27 16.0 28 14.0 29 13.0 30 29.0 31 35.0 32 65.0 33 118.0 34 204.0 35 586.0 36 2651.0 37 201.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.689646527951865 20.732013035848585 13.236400100275759 24.34194033592379 2 30.625000000000004 24.75 27.325 17.299999999999997 3 21.625 27.875 31.674999999999997 18.825 4 25.3 34.949999999999996 22.125 17.625 5 25.575 37.525 21.075 15.825 6 20.65 39.625 22.575 17.150000000000002 7 21.349999999999998 22.85 38.074999999999996 17.724999999999998 8 22.8 29.15 25.650000000000002 22.400000000000002 9 21.925 26.1 29.175 22.8 10-14 23.215 29.509999999999998 27.065 20.21 15-19 22.915 27.91 28.050000000000004 21.125 20-24 23.211963589076724 28.593578073422027 27.87336200860258 20.32109632889867 25-29 22.96648324162081 27.688844422211105 27.92896448224112 21.415707853926964 30-34 22.67907162865146 28.401360544217685 27.601040416166466 21.318527410964386 35-39 23.10193057917375 28.74362308692608 27.333199959987997 20.821246373912174 40-44 23.074229691876752 27.145858343337338 28.60144057623049 21.178471388555423 45-49 22.587905767018455 29.135197319061675 26.904416545791026 21.372480368128848 50-54 23.512053616084824 29.183755126537964 26.888066419925977 20.416124837451235 55-59 22.36118059029515 27.828914457228613 28.064032016008007 21.74587293646823 60-64 23.532059617885366 27.493247974392315 27.25817745323597 21.716514954486346 65-69 23.044608921784356 27.3004600920184 28.21564312862572 21.439287857571514 70-74 23.411705852926463 28.254127063531765 27.01350675337669 21.320660330165083 75-79 23.11540193086889 27.7574908708919 28.107648441798812 21.019458756440397 80-84 23.380845211302827 27.89697424356089 27.89197299324831 20.830207551887973 85-89 24.09704852426213 27.49374687343672 27.358679339669834 21.050525262631314 90-94 23.963387185514932 27.659680888310913 28.11984194468064 20.25708998149352 95-99 23.056917075122538 27.96338901670501 28.078423527058117 20.901270381114333 100-104 22.831415707853928 28.57928964482241 27.658829414707352 20.930465232616307 105-109 22.846423211605803 27.68384192096048 28.189094547273637 21.280640320160078 110-114 23.45703711113334 27.998399519855955 27.80334100230069 20.741222366710012 115-119 24.00200100050025 28.43421710855428 27.073536768384194 20.490245122561284 120-124 24.752376188094047 28.344172086043024 26.55327663831916 20.35017508754377 125-129 24.180881396628486 28.372767745485465 26.877094692611674 20.569256165274375 130-134 24.284570742445467 28.34200520312187 27.171302781669 20.20212127276366 135-139 25.287643821910955 27.463731865932967 27.55377688844422 19.694847423711856 140-144 25.497649294788438 27.533259977993396 26.287886365909774 20.681204361308392 145-149 26.14807403701851 27.483741870935468 26.428214107053527 19.939969984992494 150-151 25.77861163227017 27.85490931832395 26.01626016260163 20.350218886804253 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.5 8 0.5 9 0.5 10 1.5 11 1.0 12 1.0 13 1.5 14 0.5 15 0.0 16 0.0 17 1.0 18 1.0 19 0.0 20 0.5 21 1.0 22 1.5 23 2.0 24 2.0 25 2.5 26 2.5 27 5.5 28 10.0 29 13.0 30 18.5 31 25.5 32 30.0 33 37.5 34 48.5 35 63.0 36 86.0 37 115.0 38 156.5 39 177.5 40 181.0 41 222.0 42 251.0 43 248.0 44 263.0 45 263.0 46 251.0 47 241.0 48 219.5 49 200.0 50 175.0 51 141.0 52 111.0 53 92.0 54 69.0 55 55.0 56 58.5 57 43.0 58 20.0 59 15.0 60 13.0 61 11.0 62 11.0 63 9.0 64 5.0 65 2.0 66 0.5 67 0.0 68 2.0 69 2.0 70 1.0 71 1.0 72 0.5 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 1.0 83 1.5 84 0.5 85 0.5 86 0.5 87 0.5 88 0.5 89 0.0 90 0.0 91 0.5 92 0.5 93 0.5 94 1.0 95 2.0 96 1.5 97 0.5 98 1.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27499999999999997 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.03 25-29 0.05 30-34 0.04 35-39 0.03 40-44 0.04 45-49 0.034999999999999996 50-54 0.03 55-59 0.05 60-64 0.03 65-69 0.02 70-74 0.05 75-79 0.045 80-84 0.025 85-89 0.05 90-94 0.034999999999999996 95-99 0.03 100-104 0.05 105-109 0.05 110-114 0.03 115-119 0.05 120-124 0.05 125-129 0.045 130-134 0.06 135-139 0.05 140-144 0.03 145-149 0.05 150-151 0.0625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.65 #Duplication Level Percentage of deduplicated Percentage of total 1 71.04879825200291 48.775 2 18.681718863801894 25.650000000000002 3 6.5549890750182085 13.5 4 2.112163146394756 5.800000000000001 5 0.8011653313911145 2.75 6 0.5462490895848506 2.25 7 0.1820830298616169 0.8750000000000001 8 0.07283321194464676 0.4 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCAAAAAGCTCCAGCTTCAACTCACTGGTAGTGATATTTCTTACTGAACG 8 0.2 No Hit TGAAGCTCGGATTGTAGTTCTTGCAGTTCTTCGAATGATCAAAGAGAACT 8 0.2 No Hit ACAGCAGAGACACTCAAGAGGTCAAGTTAGAGCAAGGGCTTAAATTGATA 7 0.17500000000000002 No Hit GGTGGAGGTAATTTGTATGTTTTTTGTTGCATCATGGACTGGAGTTGTCA 7 0.17500000000000002 No Hit CTGAAACTGAAGCACAAATGTCTTCTCTTCTCCTACAACCTCTGCCAACT 7 0.17500000000000002 No Hit GGGAACAGCGGTGGCATTTGGATGTTTCTCAGCTGCAGCTATGTTGGCTA 7 0.17500000000000002 No Hit CCTAGTTATCGGTTTCTACCAGATGATTATCCAATATCTTCAGCAAGCCA 7 0.17500000000000002 No Hit ATTGAAGACGTGGATGAGGAGTTTCTGGATGAAGATGGAGAGGAAGGAGA 6 0.15 No Hit GATAATTATTATGCTGCTTTGAATTCTGCTGTGTTTAGTGATGGATCATT 6 0.15 No Hit CTTGATTGCCTGCTCAGAGAGTATGGGTTGCCCATCACTGGGGATGTTGA 6 0.15 No Hit AAAGCACTTGAGCCCTTCTTCAATGCTGCTGCTTCTCAGTTCATATTGGG 6 0.15 No Hit AGCTTTTATATCCCCTATTCTCTTCGTGAAGATGTCTTGCTGTGGAGGAA 6 0.15 No Hit GCCAGTGAAATTGCCATGATTGCTGAAGCAACAAGTGACAGCAGGGGTAC 6 0.15 No Hit GGAATTCATCAATGAAGTGGGAACAATGGCAAGGATCCACCATGTCAATG 6 0.15 No Hit GGTGCAGGGAAGCACATTACGGCGGGGGCCAAGAAGGTGCTCATCACAGC 6 0.15 No Hit GTGTTCCTCAGATCACTGTTTGCTTTGATATTGATGCAAACGGTATCTTG 6 0.15 No Hit CTAGGGTTTCTGCTCTTCAAAGTCACCGAAAATGACCAAGAGAACCAAGA 6 0.15 No Hit GGAAGTCGATACGACAGTATTGGGGCTTTCGAAGGAAGAAGCTTTAAAGA 6 0.15 No Hit GACGCTCTAGGCAAAATGTTCAGCTCCTTCACCATTGGTAACTGTGGCTC 6 0.15 No Hit GACAAGTTCTCATCTATTGGCTTTGGTCCCCGCCAGCTAGCTGTTATGTC 6 0.15 No Hit CCTCTCTTCACTCTCATTTTCCTCTCAAATTTCCCAAAAACCAAACACCC 6 0.15 No Hit AATTATTAATGGACTGGAGCACCTGGTTGAAGCATCACCTGTGGCAAAAC 6 0.15 No Hit AAGGGTCAGACCCTCCGCTTGCCTTCTGCCTCCATTGTCCGGTGCCGCTC 5 0.125 No Hit TATTCAAGATGCAGCGCAAGAGGAAATCACGTAGAGGTGGACCTCATTCA 5 0.125 No Hit TATTACCACAGGTAATCAAAGGAAGCCCCTGGTTGATGAAAAGGGAGAAA 5 0.125 No Hit ATTTATTGGGCGGGTTTAAGGACTCCATCAATACTTCAGTAAATCAGGGA 5 0.125 No Hit AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 5 0.125 No Hit CGCATATGTAAACATAGTGCTGGATGGGAAATTTCTTGAAATTGAGCAGT 5 0.125 No Hit GCTTGAGGCTTTTGCGAGCAGAGCAAAGATTGTGCATATTGATATTGATT 5 0.125 No Hit AGGGAGCACTGCATAGCTTATAAGCTTGTAAGAGATGGCTTCCTCCTCTA 5 0.125 No Hit CAATGAAACACATCCTCAACTTGGAGATGATGAAAATGATTGCACCACAG 5 0.125 No Hit TTTGCATACAAGAATCTGGTTTTCAAACCTGACAGGGAAGCTTTGATTGC 5 0.125 No Hit GGAAGAGAAAGAACCAGTATAGAGGAATCCGGCAGCGCCCATGGGGAAAA 5 0.125 No Hit CAAGGTAAACAAAGGACAGGCGCCACCATCCTTCACTTTGAAAGATCAGG 5 0.125 No Hit AATTCTTCACAAATGCTGGAGGGCTTGGGAAGGCTGACATTAAAAACATT 5 0.125 No Hit GGGCTCCTGGGGGGTGTAATAACCCATGTTCCGTGTTCAAAACTAACGAA 5 0.125 No Hit CTTTTGAATACACAGTTCCAAGCTAGACAGACAGACAGCAGTCGCAACTA 5 0.125 No Hit AACATCGTTTCCAAATTTAACTGTTTCTCTTTTACTGTGTTTTGCTACTC 5 0.125 No Hit AGAACGTGAATGCAGCTCAGGAAGCACTTCTCATCCGTGCCAAGGCCAAC 5 0.125 No Hit AATTTTAAGGTTGAAATAATCAAAGATCTGCCCGCAGACAAAACTATAAC 5 0.125 No Hit GGCAAAAGCAGGACAGAAGCAAGATTTCCCTAATGGTATAGCTGAATGTG 5 0.125 No Hit GTGGGTTGAACTCAGGTTGCTTTTGAATGAACAAGCCCTCATTGAAAAAC 5 0.125 No Hit TGCTGTTTGTCATGCTGGATCCCTCACTACTCCTAGTCTCCCCTGGATTT 5 0.125 No Hit GCCTCCAACTGGATTGAAGAAGTTCGAGACTCTTTCTTACCTTCCAGATC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.037500000000000006 0.0 0.0 0.0 0.0 76-77 0.0625 0.0 0.0 0.0 0.0 78-79 0.0875 0.0 0.0 0.0 0.0 80-81 0.125 0.0 0.0 0.0 0.0 82-83 0.16249999999999998 0.0 0.0 0.0 0.0 84-85 0.225 0.0 0.0 0.0 0.0 86-87 0.225 0.0 0.0 0.0 0.0 88-89 0.25 0.0 0.0 0.0 0.0 90-91 0.275 0.0 0.0 0.0 0.0 92-93 0.375 0.0 0.0 0.0 0.0 94-95 0.6875 0.0 0.0 0.0 0.0 96-97 0.75 0.0 0.0 0.0 0.0 98-99 0.7875000000000001 0.0 0.0 0.0 0.0 100-101 0.975 0.0 0.0 0.0 0.0 102-103 1.075 0.0 0.0 0.0 0.0 104-105 1.3125 0.0 0.0 0.0 0.0 106-107 1.55 0.0 0.0 0.0 0.0 108-109 1.875 0.0 0.0 0.0 0.0 110-111 2.0 0.0 0.0 0.0 0.0 112-113 2.325 0.0 0.0 0.0 0.0 114-115 2.6625 0.0 0.0 0.0 0.0 116-117 3.0 0.0 0.0 0.0 0.0 118-119 3.525 0.0 0.0 0.0 0.0 120-121 4.025 0.0 0.0 0.0 0.0 122-123 4.512499999999999 0.0 0.0 0.0 0.0 124-125 5.15 0.0 0.0 0.0 0.0 126-127 5.512499999999999 0.0 0.0 0.0 0.0 128-129 6.025 0.0 0.0 0.0 0.0 130-131 6.525 0.0 0.0 0.0 0.0 132-133 7.1875 0.0 0.0 0.0 0.0 134-135 7.9625 0.0 0.0 0.0 0.0 136-137 8.4125 0.0 0.0 0.0 0.0 138-139 9.025 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 817602 spots for SRR13695421.sra Written 817602 spots for SRR13695421.sra Read 817602 spots for SRR13695421.sra Written 817602 spots for SRR13695421.sra Read 817602 spots for SRR13695421.sra Written 817602 spots for SRR13695421.sra Read 817602 spots for SRR13695421.sra Written 817602 spots for SRR13695421.sra Read 817602 spots for SRR13695421.sra Written 817602 spots for SRR13695421.sra Read 817602 spots for SRR13695421.sra Written 817602 spots for SRR13695421.sra Read 817602 spots for SRR13695421.sra Written 817602 spots for SRR13695421.sra Read 817602 spots for SRR13695421.sra Written 817602 spots for SRR13695421.sra Read 817602 spots for SRR13695421.sra Written 817602 spots for SRR13695421.sra Read 817602 spots for SRR13695421.sra Written 817602 spots for SRR13695421.sra Read 817602 spots for SRR13695421.sra Written 817602 spots for SRR13695421.sra Read 817602 spots for SRR13695421.sra Written 817602 spots for SRR13695421.sra Read 817602 spots for SRR13695421.sra Written 817602 spots for SRR13695421.sra Read 817602 spots for SRR13695421.sra Written 817602 spots for SRR13695421.sra Read 817602 spots for SRR13695421.sra Written 817602 spots for SRR13695421.sra Read 817602 spots for SRR13695421.sra Written 817602 spots for SRR13695421.sra Read 817602 spots for SRR13695421.sra Written 817602 spots for SRR13695421.sra Read 817602 spots for SRR13695421.sra Written 817602 spots for SRR13695421.sra Read 817613 spots for SRR13695421.sra Written 817613 spots for SRR13695421.sra Read 817602 spots for SRR13695421.sra Written 817602 spots for SRR13695421.sra SRR ids: ['SRR13695421.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_r7y3koyj SRR13695421.sra spots: 16352051 blocks: [[1, 817602], [817603, 1635204], [1635205, 2452806], [2452807, 3270408], [3270409, 4088010], [4088011, 4905612], [4905613, 5723214], [5723215, 6540816], [6540817, 7358418], [7358419, 8176020], [8176021, 8993622], [8993623, 9811224], [9811225, 10628826], [10628827, 11446428], [11446429, 12264030], [12264031, 13081632], [13081633, 13899234], [13899235, 14716836], [14716837, 15534438], [15534439, 16352051]] SRR13695421 file size 5535441 SRR13695421 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695421 SRR13695421_1.fastq SRR13695421_2.fastq Input file: SRR13695421_1.fastq Paired file: SRR13695421_2.fastq trimmed: SRR13695421-trimmed-pair1.fastq, SRR13695421-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 01:32:57 2025 >> started Wed Feb 12 01:33:16 2025 >> done (18.267s) 16352051 read pairs processed; of these: 105 ( 0.00%) short read pairs filtered out after trimming by size control 1974 ( 0.01%) empty read pairs filtered out after trimming by size control 16349972 (99.99%) read pairs available; of these: 1980015 (12.11%) trimmed read pairs available after processing 14369957 (87.89%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 4 0.00% 20 1 0.00% 21 2 0.00% 22 2 0.00% 23 4 0.00% 24 1 0.00% 25 2 0.00% 26 3 0.00% 27 9 0.00% 28 2 0.00% 29 3 0.00% 30 3 0.00% 31 4 0.00% 32 1 0.00% 33 6 0.00% 34 3 0.00% 35 1 0.00% 36 2 0.00% 37 6 0.00% 38 4 0.00% 39 14 0.00% 40 10 0.00% 41 8 0.00% 42 17 0.00% 43 19 0.00% 44 4 0.00% 45 19 0.00% 46 25 0.00% 47 32 0.00% 48 30 0.00% 49 42 0.00% 50 49 0.00% 51 46 0.00% 52 43 0.00% 53 65 0.00% 54 78 0.00% 55 80 0.00% 56 84 0.00% 57 100 0.00% 58 133 0.00% 59 142 0.00% 60 203 0.00% 61 250 0.00% 62 256 0.00% 63 282 0.00% 64 330 0.00% 65 432 0.00% 66 446 0.00% 67 548 0.00% 68 611 0.00% 69 714 0.00% 70 810 0.00% 71 977 0.01% 72 1191 0.01% 73 1395 0.01% 74 1482 0.01% 75 1792 0.01% 76 1940 0.01% 77 2115 0.01% 78 2230 0.01% 79 2697 0.02% 80 3008 0.02% 81 3528 0.02% 82 3870 0.02% 83 4382 0.03% 84 4924 0.03% 85 5403 0.03% 86 5718 0.03% 87 6106 0.04% 88 6642 0.04% 89 7154 0.04% 90 7635 0.05% 91 8506 0.05% 92 9038 0.06% 93 10028 0.06% 94 10605 0.06% 95 11666 0.07% 96 12097 0.07% 97 12642 0.08% 98 12908 0.08% 99 13845 0.08% 100 14741 0.09% 101 15193 0.09% 102 16622 0.10% 103 17044 0.10% 104 18048 0.11% 105 19244 0.12% 106 19952 0.12% 107 20731 0.13% 108 21009 0.13% 109 21753 0.13% 110 22109 0.14% 111 23208 0.14% 112 24246 0.15% 113 25032 0.15% 114 25976 0.16% 115 27247 0.17% 116 28255 0.17% 117 29043 0.18% 118 29710 0.18% 119 30140 0.18% 120 30977 0.19% 121 32089 0.20% 122 32683 0.20% 123 33805 0.21% 124 35296 0.22% 125 35768 0.22% 126 37307 0.23% 127 38532 0.24% 128 38408 0.23% 129 38702 0.24% 130 40057 0.24% 131 39991 0.24% 132 40622 0.25% 133 41935 0.26% 134 42591 0.26% 135 43663 0.27% 136 44683 0.27% 137 45465 0.28% 138 46110 0.28% 139 47354 0.29% 140 47074 0.29% 141 47723 0.29% 142 48484 0.30% 143 49120 0.30% 144 51000 0.31% 145 50884 0.31% 146 52324 0.32% 147 53298 0.33% 148 53740 0.33% 149 54599 0.33% 150 54918 0.34% 151 14369957 87.89% 16349972 reads passed initial QC criterion=sequence-density sequence-density=0.40 sequence-density-rank=1 fanout-score=2.22 fanout-score-rank=24 prefix-density=0.41 prefix-fanout=2.1 sequence=TGAGATGCCTCAGTGCATCCAAACATGGGTAG criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=426.18 fanout-score-rank=1 prefix-density=0.28 prefix-fanout=19.4 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGTTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGG criterion=sequence-density sequence-density=0.75 sequence-density-rank=1 fanout-score=2.24 fanout-score-rank=24 prefix-density=0.77 prefix-fanout=2.2 sequence=CCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAATCATCATGTCTAGCACCTGCGACAACTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAGTGAACAAACAAAAGTGTCCGTGAGTGTCTTTATCTTTGTGTCTTCTTCA criterion=fanout-score sequence-density=0.15 sequence-density-rank=22 fanout-score=28.11 fanout-score-rank=1 prefix-density=0.37 prefix-fanout=11.2 sequence=AAAGAAAAGAAAA SRR13695421 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 01:33:54 Started mapping on | Feb 12 01:33:54 Finished on | Feb 12 01:35:35 Mapping speed, Million of reads per hour | 582.77 Number of input reads | 16349972 Average input read length | 296 UNIQUE READS: Uniquely mapped reads number | 15289330 Uniquely mapped reads % | 93.51% Average mapped length | 294.48 Number of splices: Total | 14837534 Number of splices: Annotated (sjdb) | 14508939 Number of splices: GT/AG | 14528439 Number of splices: GC/AG | 241554 Number of splices: AT/AC | 8129 Number of splices: Non-canonical | 59412 Mismatch rate per base, % | 0.37% Deletion rate per base | 0.03% Deletion average length | 2.93 Insertion rate per base | 0.02% Insertion average length | 2.28 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 355338 % of reads mapped to multiple loci | 2.17% Number of reads mapped to too many loci | 67378 % of reads mapped to too many loci | 0.41% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.77% % of reads unmapped: other | 0.13% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 705512 705512 705512 N_multimapping 355338 355338 355338 N_noFeature 629571 14867707 907735 N_ambiguous 241225 1803 96340 UnstrandedReadsAssigned:14418534 PositiveStrandReadsAssigned:419820 NegativeStrandReadsAssigned:14285255 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695421 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695421-trimmed-pair1.fastq SRR13695421-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 16,349,972 reads, 14,323,367 reads pseudoaligned [quant] estimated average fragment length: 249.455 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,013 rounds 52401 SRR13695421.ke.tsv 34699 SRR13695421.se.tsv 87100 total ==> SRR13695421.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1769.54 522 18.5878 Potri.005G024800.1.v4.1 1035 786.545 251 20.108 Potri.004G059700.1.v4.1 961 712.622 5 0.44211 Potri.007G009000.2.v4.1 1416 1167.54 0 0 Potri.003G141000.2.v4.1 2943 2694.54 860 20.1109 Potri.016G087400.1.v4.1 270 86.5127 615.702 448.445 Potri.015G069301.1.v4.1 564 325.716 0 0 Potri.010G195200.1.v4.1 1773 1524.54 83.9377 3.46925 Potri.012G127500.1.v4.1 977 728.596 46 3.97823 ==> SRR13695421.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 195 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 175 Potri.001G212900.v4.1 44 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 9 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR13695421 completed mapping pipeline successfully