Starting /dee2/code/volunteer_pipeline.sh SRR13695422 current disk space = 3050924068864 free memory = 1166885084 SRR13695422 SRAfilesize efc5d08104b6436d2c1cb9cc93be233c SRR13695422.sra SRR13695422.sra file validated SRR13695422 is paired end SRR13695422 is conventional basespace SRR13695422 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695422_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.6245 37.0 37.0 37.0 37.0 37.0 2 36.2685 37.0 37.0 37.0 37.0 37.0 3 36.591 37.0 37.0 37.0 37.0 37.0 4 36.5775 37.0 37.0 37.0 37.0 37.0 5 36.59 37.0 37.0 37.0 37.0 37.0 6 36.6205 37.0 37.0 37.0 37.0 37.0 7 36.4565 37.0 37.0 37.0 37.0 37.0 8 36.433 37.0 37.0 37.0 37.0 37.0 9 36.5545 37.0 37.0 37.0 37.0 37.0 10-14 36.57340000000001 37.0 37.0 37.0 37.0 37.0 15-19 36.496500000000005 37.0 37.0 37.0 37.0 37.0 20-24 36.541199999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.476200000000006 37.0 37.0 37.0 37.0 37.0 30-34 36.4863 37.0 37.0 37.0 37.0 37.0 35-39 36.4005 37.0 37.0 37.0 37.0 37.0 40-44 36.4311 37.0 37.0 37.0 37.0 37.0 45-49 36.3635 37.0 37.0 37.0 37.0 37.0 50-54 36.3806 37.0 37.0 37.0 37.0 37.0 55-59 36.3641 37.0 37.0 37.0 37.0 37.0 60-64 36.3598 37.0 37.0 37.0 37.0 37.0 65-69 36.2818 37.0 37.0 37.0 37.0 37.0 70-74 36.314800000000005 37.0 37.0 37.0 37.0 37.0 75-79 36.2726 37.0 37.0 37.0 37.0 37.0 80-84 36.2241 37.0 37.0 37.0 37.0 37.0 85-89 36.2305 37.0 37.0 37.0 37.0 37.0 90-94 36.165 37.0 37.0 37.0 37.0 37.0 95-99 36.1465 37.0 37.0 37.0 37.0 37.0 100-104 36.1509 37.0 37.0 37.0 37.0 37.0 105-109 36.0565 37.0 37.0 37.0 37.0 37.0 110-114 36.045399999999994 37.0 37.0 37.0 37.0 37.0 115-119 36.1008 37.0 37.0 37.0 37.0 37.0 120-124 35.9501 37.0 37.0 37.0 37.0 37.0 125-129 35.9701 37.0 37.0 37.0 37.0 37.0 130-134 35.9222 37.0 37.0 37.0 37.0 37.0 135-139 35.9169 37.0 37.0 37.0 37.0 37.0 140-144 35.8164 37.0 37.0 37.0 37.0 37.0 145-149 35.660700000000006 37.0 37.0 37.0 37.0 37.0 150-151 35.6025 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 1.0 24 4.0 25 2.0 26 9.0 27 8.0 28 8.0 29 11.0 30 32.0 31 43.0 32 32.0 33 59.0 34 143.0 35 358.0 36 2981.0 37 309.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.75 11.75 8.05 46.45 2 18.960321446509294 14.816675037669514 37.29281767955801 28.930185836263185 3 16.925 17.9 27.075 38.1 4 20.674999999999997 24.099999999999998 24.55 30.675 5 22.625 31.724999999999998 24.8 20.849999999999998 6 21.425 35.375 24.0 19.2 7 17.05 29.225 37.55 16.175 8 17.175 25.4 35.025 22.400000000000002 9 17.599999999999998 23.275000000000002 36.0 23.125 10-14 19.12 30.725 27.35 22.805 15-19 19.425 28.84 28.470000000000002 23.265 20-24 19.685 28.365000000000002 28.62 23.330000000000002 25-29 19.78 28.244999999999997 28.415000000000003 23.56 30-34 19.585 27.935 28.205000000000002 24.275 35-39 19.744999999999997 28.33 27.71 24.215 40-44 19.38 28.360000000000003 27.985 24.275 45-49 19.994999999999997 28.78 27.93 23.294999999999998 50-54 20.155 28.675 28.005000000000003 23.165 55-59 20.505000000000003 28.754999999999995 27.134999999999998 23.605 60-64 20.57 28.42 27.034999999999997 23.974999999999998 65-69 20.005 28.34 27.650000000000002 24.005000000000003 70-74 20.630000000000003 27.665 28.065 23.64 75-79 20.03 28.294999999999998 27.54 24.135 80-84 20.424999999999997 28.675 28.09 22.81 85-89 19.18 28.15 28.025 24.645 90-94 20.805 27.834999999999997 27.495000000000005 23.865 95-99 20.8 27.445000000000004 27.975 23.78 100-104 20.75 28.299999999999997 28.139999999999997 22.81 105-109 20.59 28.075 28.185 23.150000000000002 110-114 20.895 27.345000000000002 28.384999999999998 23.375 115-119 21.395 28.449999999999996 27.339999999999996 22.814999999999998 120-124 20.74 29.21 26.290000000000003 23.76 125-129 20.845 28.95 26.889999999999997 23.315 130-134 20.41 28.689999999999998 26.75 24.15 135-139 21.4 27.994999999999997 26.965 23.64 140-144 21.485000000000003 27.12 26.935 24.46 145-149 21.615000000000002 27.77 27.634999999999998 22.98 150-151 21.7375 28.3625 25.624999999999996 24.275 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.0 15 1.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 1.0 22 1.0 23 1.0 24 1.5 25 2.0 26 3.0 27 3.5 28 7.5 29 15.0 30 24.0 31 26.0 32 36.0 33 46.0 34 50.5 35 71.0 36 97.5 37 119.5 38 146.0 39 162.5 40 180.0 41 197.0 42 237.0 43 270.5 44 253.5 45 254.0 46 269.0 47 258.0 48 239.0 49 214.0 50 162.5 51 132.5 52 110.0 53 92.0 54 85.0 55 62.5 56 51.0 57 48.0 58 28.5 59 12.5 60 10.5 61 7.0 62 3.0 63 2.5 64 2.5 65 1.0 66 0.5 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.44999999999999996 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.475 #Duplication Level Percentage of deduplicated Percentage of total 1 68.74764949229034 45.7 2 19.51861602106055 25.95 3 7.145543437382475 14.249999999999998 4 2.8958254983076346 7.7 5 0.9402030838661152 3.125 6 0.4889056036103798 1.95 7 0.11282437006393381 0.525 8 0.15043249341857842 0.8 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTCTCTGGCTTGCGTTCTGTTCCTTTGGTGAACCTTGCAAACTTCAAATT 8 0.2 No Hit CTCCAATGGATTTGATAGCTCGTGCATCTTCCTCCTCGTCTTGGCTTTGA 8 0.2 No Hit ACCTGTGATAGGATAACAATCTGCTCCCCTTTGAACCTTGCCAGAGAATA 8 0.2 No Hit CCCATTTTATCGAAATGAGTATTAGGTCTTATTACCATATCAATGGTCTT 8 0.2 No Hit GTGAGAATTTAGCACATTCTGCATGACTTTTTGTTCCAAACTTGAGCAAT 7 0.17500000000000002 No Hit GTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGAC 7 0.17500000000000002 No Hit CTTGAGAAAAGAGAGCCTTAAAATCATTGGCTGGGGTGCCAGTTCCCTCT 7 0.17500000000000002 No Hit GGCAGCTGCAGCAGCGCCCGATGATATTCCAACTAGCAAACCTTCTTTCA 6 0.15 No Hit CCTTGAGTATCATCTCCACTCCGGCAAGGCAGCCCAACAATTGCAACTCG 6 0.15 No Hit ATCCGTTCTTGCATCTCCTTCAACTCCGCGCACAGTGCAATACACAGTCT 6 0.15 No Hit TGATGCACTCATTCCCTTGCATTTTTCCTCCACGCTGGGCTTTCCCTCCA 6 0.15 No Hit GTGCTAATATGACTACGGCATCCTCCTCATCCCATGCGTTGATAGCGTGT 6 0.15 No Hit CTTCAACGCAACTACATCAAGCCAACCACAACGACGAGGTCGACCAGTAG 6 0.15 No Hit CTGGCTCTGGAGCTTGACCTTGATGGTGTCAAAGGGGTGCCCAACTAACA 6 0.15 No Hit TATAAATTCAAACAAAAAGAAAACAGACACAAGTATACTACACTCTAATT 6 0.15 No Hit CTCCACCATAACTGCCATTAGAGGCCAATCTGGGAAGGCATTCACTTTAC 6 0.15 No Hit CTGCGTTCATTTTTTGAAGTTCCACTTGCAATTGTGCTAGCTCAACATTC 6 0.15 No Hit GAGATATTCACAAGGCGTGACAGATTTTGCCTCCAGCCTGCTGCATAAGA 6 0.15 No Hit GACAGCAATGGCTTCCTGTTTAATAAGATCCTTTACGAAACTGTTAGTTC 6 0.15 No Hit GTCAAGGAGAGATGGGTTTTGGGTTTTAGAGAGAAAGGTGATAAAGAGTG 6 0.15 No Hit ATCTTTTTGCCAGAGCCCAGGTACAATTTGAACAAAGCAACCCTAACAGA 5 0.125 No Hit GTCCAAAATAAGCAATATATGGGCAAGGATGAGTAGCAGATGATACTGCT 5 0.125 No Hit CAGCCCTTGAGATGGAATATGCTGCTGCTAAGCTTGGTGTTGCCTTCTCA 5 0.125 No Hit CCCGGAGTTTGGCTCCTTTGGTTTAGGAGAGGCTTCTGTCAGCGGATGGC 5 0.125 No Hit GTAAGCAACAAGGCGATCGTCTTCCTCCTTGGTCCACGCACCCTTGTTTG 5 0.125 No Hit GTAGAAATGGTGCTTGGCAGAGAGCAACCACCTGGCGAGGAAAGGTAAGT 5 0.125 No Hit GGGATTCGTCTGATTGGAGAGAAGGCAGATCTAATGACTGCAAAGGCTTC 5 0.125 No Hit GGCCTCTTGGAGATGTATCAGGAGCGATCAGGACAATGCCCTCTGTCGAA 5 0.125 No Hit GTAGAAGTATATGCCAGCAAGAGCTACAATTCCAACCACCACTGCAATTG 5 0.125 No Hit CCTGGGGCAAGACTTGTTTTTATCCATGGTTTGACCTTTAAACCAAGTTC 5 0.125 No Hit GTCCTTGCTTTGTGATCACATATATTAAGCCATACTTTTGGGATATCTGC 5 0.125 No Hit CCTAAAATAAGACATTTATAAAGCAGGAAAAAGCCACTGTTCAGAACAAA 5 0.125 No Hit CATTTCTTCCCACTTTTCTTCAGCCCAGAACCTCCAAAGCTCCCTTTCTG 5 0.125 No Hit CACCAATTGAAGTACAGATGAAAGGACCAGCAAATGCAAAGCATTTGGGA 5 0.125 No Hit GGTCAAGTGCATAATCTATATTCTGGAACAATAGTTGTTTGTTGCTTAAT 5 0.125 No Hit GCAGCCTTTTCCTTGTCAGTGAAAACTCCAGTTGACTCCACAATATACTC 5 0.125 No Hit CCACCATAATTATCACTATTGAAAGAACGACCCCTAGTATTGCTACCGTA 5 0.125 No Hit CTACCTTTCATTGCAGAAACAATAAGCAAACGGGTTGAGTTCAAGCGAGG 5 0.125 No Hit TCCAAAGGCAATTCCCATTGCTAACTGTGAACTTGATTCCGGGTGATACT 5 0.125 No Hit AAGTGGTTCAATTTCATTGACTGGAATCCCTGTCTCGATGTTGAGCTGTG 5 0.125 No Hit CCCGTATGGAGACTAATGCTCCAACATCTAAGCGTTCGATATTTTCTATA 5 0.125 No Hit CACATTAACAACTAAAAAGCACACATGGAGATTGACACCCTACTGCCAAA 5 0.125 No Hit CCCAAAAGCATACACCAGAAAAGTAGTCGTGCCAGATAGTCCCTTGTCAA 5 0.125 No Hit GGGGGTAGAAATATTTCAATGTTTATTTTGCCATTCCCACCATTACTTGA 5 0.125 No Hit CATTAAATCAAAGAAATGGCAGCACTTCTCCACCAGAGTACCCCCTGTGT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0125 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.2 0.0 0.0 0.0 0.0 68-69 0.2 0.0 0.0 0.0 0.0 70-71 0.2 0.0 0.0 0.0 0.0 72-73 0.2 0.0 0.0 0.0 0.0 74-75 0.2 0.0 0.0 0.0 0.0 76-77 0.2 0.0 0.0 0.0 0.0 78-79 0.2 0.0 0.0 0.0 0.0 80-81 0.225 0.0 0.0 0.0 0.0 82-83 0.225 0.0 0.0 0.0 0.0 84-85 0.2625 0.0 0.0 0.0 0.0 86-87 0.3 0.0 0.0 0.0 0.0 88-89 0.525 0.0 0.0 0.0 0.0 90-91 0.55 0.0 0.0 0.0 0.0 92-93 0.6125 0.0 0.0 0.0 0.0 94-95 0.675 0.0 0.0 0.0 0.0 96-97 0.825 0.0 0.0 0.0 0.0 98-99 1.0375 0.0 0.0 0.0 0.0 100-101 1.3250000000000002 0.0 0.0 0.0 0.0 102-103 1.5625 0.0 0.0 0.0 0.0 104-105 1.75 0.0 0.0 0.0 0.0 106-107 2.0375 0.0 0.0 0.0 0.0 108-109 2.2874999999999996 0.0 0.0 0.0 0.0 110-111 2.4749999999999996 0.0 0.0 0.0 0.0 112-113 2.7125 0.0 0.0 0.0 0.0 114-115 3.2625 0.0 0.0 0.0 0.0 116-117 3.5625 0.0 0.0 0.0 0.0 118-119 4.0 0.0 0.0 0.0 0.0 120-121 4.300000000000001 0.0 0.0 0.0 0.0 122-123 4.737500000000001 0.0 0.0 0.0 0.0 124-125 5.1 0.0 0.0 0.0 0.0 126-127 5.35 0.0 0.0 0.0 0.0 128-129 5.775 0.0 0.0 0.0 0.0 130-131 6.2375 0.0 0.0 0.0 0.0 132-133 6.5875 0.0 0.0 0.0 0.0 134-135 6.9125 0.0 0.0 0.0 0.0 136-137 7.4125 0.0 0.0 0.0 0.0 138-139 8.1375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCACGAG 10 0.006830828 145.0 1 AAAAAAA 50 0.0013298223 17.4 75-79 >>END_MODULE SRR13695422 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695422_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.1575 37.0 37.0 37.0 37.0 37.0 2 36.2315 37.0 37.0 37.0 37.0 37.0 3 36.238 37.0 37.0 37.0 37.0 37.0 4 36.302 37.0 37.0 37.0 37.0 37.0 5 36.3995 37.0 37.0 37.0 37.0 37.0 6 36.2705 37.0 37.0 37.0 37.0 37.0 7 36.314 37.0 37.0 37.0 37.0 37.0 8 36.311 37.0 37.0 37.0 37.0 37.0 9 36.335 37.0 37.0 37.0 37.0 37.0 10-14 36.3322 37.0 37.0 37.0 37.0 37.0 15-19 36.298199999999994 37.0 37.0 37.0 37.0 37.0 20-24 36.2701 37.0 37.0 37.0 37.0 37.0 25-29 36.200500000000005 37.0 37.0 37.0 37.0 37.0 30-34 36.19175 37.0 37.0 37.0 37.0 37.0 35-39 36.1081 37.0 37.0 37.0 37.0 37.0 40-44 36.170500000000004 37.0 37.0 37.0 37.0 37.0 45-49 36.16345 37.0 37.0 37.0 37.0 37.0 50-54 36.10015 37.0 37.0 37.0 37.0 37.0 55-59 36.0615 37.0 37.0 37.0 37.0 37.0 60-64 36.05 37.0 37.0 37.0 37.0 37.0 65-69 35.995650000000005 37.0 37.0 37.0 37.0 37.0 70-74 35.9713 37.0 37.0 37.0 37.0 37.0 75-79 36.021100000000004 37.0 37.0 37.0 37.0 37.0 80-84 35.98545 37.0 37.0 37.0 37.0 37.0 85-89 35.842150000000004 37.0 37.0 37.0 37.0 37.0 90-94 35.9034 37.0 37.0 37.0 37.0 37.0 95-99 35.90575 37.0 37.0 37.0 37.0 37.0 100-104 35.80650000000001 37.0 37.0 37.0 37.0 37.0 105-109 35.76915 37.0 37.0 37.0 37.0 37.0 110-114 35.7726 37.0 37.0 37.0 37.0 37.0 115-119 35.6764 37.0 37.0 37.0 37.0 37.0 120-124 35.6343 37.0 37.0 37.0 37.0 37.0 125-129 35.69495 37.0 37.0 37.0 37.0 37.0 130-134 35.506099999999996 37.0 37.0 37.0 37.0 37.0 135-139 35.506299999999996 37.0 37.0 37.0 37.0 37.0 140-144 35.3583 37.0 37.0 37.0 34.6 37.0 145-149 35.24395 37.0 37.0 37.0 32.2 37.0 150-151 34.996875 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 1.0 13 1.0 14 1.0 15 0.0 16 0.0 17 0.0 18 3.0 19 1.0 20 0.0 21 2.0 22 4.0 23 2.0 24 2.0 25 4.0 26 8.0 27 7.0 28 10.0 29 19.0 30 21.0 31 45.0 32 60.0 33 114.0 34 218.0 35 571.0 36 2704.0 37 201.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.37029603612644 21.04867034621174 13.271450075263422 31.309583542398396 2 27.224999999999998 26.700000000000003 31.025000000000002 15.049999999999999 3 19.2 29.025000000000002 30.825000000000003 20.95 4 23.95 34.525 22.85 18.675 5 24.175 37.125 21.75 16.950000000000003 6 20.825 40.175 22.225 16.775000000000002 7 20.375 21.349999999999998 37.5 20.775 8 22.05 24.725 29.4 23.825 9 20.0 24.95 31.674999999999997 23.375 10-14 22.515 29.615000000000002 27.13 20.74 15-19 21.66 28.860000000000003 27.63 21.85 20-24 22.361708512553765 28.95368610583175 27.62328698609583 21.061318395518654 25-29 21.925962981490745 28.569284642321165 29.004502251125565 20.50025012506253 30-34 22.437853248637023 28.484969739408793 28.35992597409093 20.717251037863253 35-39 22.37671301390417 28.4185255576673 28.063419025707713 21.141342402720817 40-44 22.519007603041217 28.566426570628252 27.81112444977991 21.10344137655062 45-49 21.64757665182814 28.294903216125643 29.09018156354724 20.967338568498974 50-54 21.710427606901725 27.991997999499873 27.89197299324831 22.405601400350086 55-59 22.446223111555778 28.254127063531765 28.484242121060532 20.815407703851925 60-64 22.99189756927078 28.443533059917975 27.87336200860258 20.691207362208665 65-69 23.31082770692673 28.257064266066518 27.84196049012253 20.59014753688422 70-74 23.47173586793397 27.938969484742373 27.443721860930463 21.145572786393195 75-79 22.078831532613044 27.55102040816326 27.911164465786314 22.458983593437377 80-84 23.15578894723681 28.287071767941985 27.131782945736433 21.42535633908477 85-89 23.21044470011505 27.832524636086237 27.682457105697566 21.274573558101146 90-94 23.331999599879964 27.768330499149744 28.293488046413923 20.606181854556365 95-99 23.460865216304075 27.991997999499873 27.191797949487373 21.355338834708675 100-104 23.1615807903952 27.908954477238616 27.673836918459227 21.25562781390695 105-109 23.495573007853533 27.84252913811215 27.507378320244108 21.154519533790207 110-114 23.56707012103631 28.33850155046514 27.573271981594477 20.52115634690407 115-119 23.88955582232893 27.541016406562623 28.186274509803923 20.38315326130452 120-124 24.312156078039017 28.394197098549274 27.388694347173587 19.90495247623812 125-129 24.711120004001803 28.32274523535591 26.531939372717723 20.434195387924568 130-134 25.222655859101373 27.864505153607528 26.87381166816772 20.039027319123388 135-139 25.405243145887535 27.41144686812087 27.77166299779868 19.411646988192917 140-144 25.487646293888165 27.333199959987997 27.63829148744623 19.540862258677603 145-149 26.27445094802141 26.60963529941468 27.445094802141178 19.670818950422735 150-151 25.497310146378084 28.08707619166771 27.13624421368698 19.279369448267232 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 0.0 15 1.0 16 1.5 17 0.5 18 0.5 19 0.5 20 0.0 21 1.0 22 1.0 23 1.5 24 3.5 25 2.5 26 2.5 27 6.5 28 9.0 29 14.0 30 19.5 31 27.0 32 31.5 33 41.5 34 59.5 35 77.5 36 98.5 37 116.5 38 140.5 39 166.0 40 192.5 41 219.0 42 256.0 43 297.0 44 287.0 45 260.0 46 254.0 47 221.0 48 200.0 49 210.0 50 183.0 51 153.0 52 118.0 53 74.0 54 58.0 55 44.5 56 41.0 57 37.5 58 17.5 59 10.5 60 12.0 61 6.5 62 6.5 63 5.5 64 1.5 65 1.0 66 1.0 67 0.5 68 0.5 69 1.0 70 1.0 71 0.0 72 0.0 73 0.0 74 0.5 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.5 81 0.5 82 1.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35000000000000003 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.03 25-29 0.05 30-34 0.034999999999999996 35-39 0.03 40-44 0.04 45-49 0.034999999999999996 50-54 0.025 55-59 0.05 60-64 0.03 65-69 0.025 70-74 0.05 75-79 0.04 80-84 0.025 85-89 0.045 90-94 0.03 95-99 0.025 100-104 0.05 105-109 0.045 110-114 0.03 115-119 0.04 120-124 0.05 125-129 0.045 130-134 0.06999999999999999 135-139 0.06 140-144 0.03 145-149 0.055 150-151 0.08750000000000001 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.05 #Duplication Level Percentage of deduplicated Percentage of total 1 69.03860711582135 45.6 2 19.076457229371687 25.2 3 7.040121120363361 13.950000000000001 4 2.9901589704769114 7.9 5 0.984102952308857 3.25 6 0.3785011355034065 1.5 7 0.22710068130204392 1.05 8 0.18925056775170326 1.0 9 0.0 0.0 >10 0.0757002271006813 0.5499999999999999 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATC 12 0.3 No Hit ATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAAT 10 0.25 No Hit CCTAGAGTGCATTTGGTTCCATATCATGTTGATGGGGGTCAGCCTTCGTA 8 0.2 No Hit AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 8 0.2 No Hit CGAAGTCATTTCTTATCCCAGGGCTGTGGATCCTAACAGCCGCATGTGGC 8 0.2 No Hit CAAAATTTCAAAATTCTTCCAAAACATAAAGCTTAAACCGACAGATATTT 8 0.2 No Hit GAATTACCAGATATCTGTTCCTCATAACGAAACAGATAAAATAGCTCGAG 8 0.2 No Hit CTTGTTCTACTTGGGACATCAATGCTATATAGAACATACTTATTGCATTA 7 0.17500000000000002 No Hit TTGTAATCCATCTGTTTTATAATGCTTTATAGTTCCAAACGTTGCCTGCT 7 0.17500000000000002 No Hit GGAAAATCAATTTCGGGCTTTATAATTCTCTTTGTTCATACATTTCTATT 7 0.17500000000000002 No Hit CACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATC 7 0.17500000000000002 No Hit AACAAACCTAAATCATCCCTTGAACAGTTTGGGTAATGGGAATATACTGG 7 0.17500000000000002 No Hit GTGCTCAGTCCTGACTCAAGCTTCTTCCAAATTGAAGAAAGATGGCCACC 7 0.17500000000000002 No Hit AATGGAAATAAACAACCAGATGTGCCCATATTTTCCATGACGAGACCTTC 6 0.15 No Hit TGATCACAAAAGCAGCATTTGATAAATCTAAAAATGGTCCTCGCAAGGGA 6 0.15 No Hit GAACGCAGTTAGCCTCCATCCAAAATTTATAATGGCCAAAGCTATAACGG 6 0.15 No Hit AGTGGGCGGAAATTACAAAGGCAGGGTGCTATATCTCTATCGAACCGTTC 6 0.15 No Hit CAGCTGGTGGGATCTGTACTGGTCTTGGCATTGCCCCCCGAGTTGTTGGT 6 0.15 No Hit CCTCCTCTCTCTATTTCTCTCTCAAAATGTCTGCTGCATCTGCTTTCACC 6 0.15 No Hit ATCAAGGCGAAGACACCCAATGCTTATATCCTCCAGCAGTTTGAAAACCC 6 0.15 No Hit GAATTCTTAAATCTCACCAGCTCCCTACCCCCTCTTACCACTACCTTTTT 6 0.15 No Hit AGTCACTCTACCCTTCGCATTACTTCCTCAATCAACCACTGCTGTTTGAT 6 0.15 No Hit AAAAGGAGGAATGGTTCAGTGACACAGTGACTGCAGTTCTTGTTCCCCCA 6 0.15 No Hit AGTTAACCCTCATCCATATTTATCTCTTTTATTGATGGCATTTTATTGCG 5 0.125 No Hit GTTAGGTTCCTTCCAGTTTCGTTCCTCCTTCGAATCGTAGAAAATCAAAC 5 0.125 No Hit ATCCGAACCAACATTCCCTGAATTAAAAGAAACGATCCATTCTGTAGTCC 5 0.125 No Hit GCTGTACGCAATCAGTGAAGGGCAGGGATCTTTTGATCTGTCATGAGTTT 5 0.125 No Hit CAACACATTTGCCATCACTAAATCTAGTACTGGTAGAAAAGTTGATGACC 5 0.125 No Hit ATGAGCTCTTCGTGGGACGTGTTGCCATGATTGGATTTGCTGCATCGTTG 5 0.125 No Hit GAAATGGAAACAAAGCCAAGTGAGATCAGTAGCTCAAAGATGTTTGGTGG 5 0.125 No Hit GGTCACTCCATGTTTGTCTAATATAGTATTTGCTGTAAATTAAAGTACAG 5 0.125 No Hit GAAGCCGTTGTGCACCACTGTTGCTGACTGCAAGAAGGTAGTTGATGCTG 5 0.125 No Hit CCACACCACAGGGCTAGAATGGCAACAATAGCTGGTCTTAACCTCTCAAC 5 0.125 No Hit GCTTCCTCCTCTATGATCTCATCGGCAGCCGTTGCCACCGTCAACCGCAC 5 0.125 No Hit GGAGACTAAAAGGATTCTTCCATCCGTGACATTATCAGTCACTGTTCCTC 5 0.125 No Hit TTAGAAGTGCCTTTCTTAATTGATGACTTTATCCTGGTTGATCCTTTTAT 5 0.125 No Hit GGAAACCCTTTGACAAACAGCCTGATAATTACTTCTGCCCTGTTTGTGGT 5 0.125 No Hit TGAAGACACTCATGTGATCAATGGAAAACAGGTAGAGATTAAAAGGACTA 5 0.125 No Hit CTCCAGAGAGGCCACAATTGGTCAAAGGGAATATGCAGCTTTTCTCTGTG 5 0.125 No Hit GGAATGGCAGCAACAGCTGTCGGGGTTTATTGCGCTAGCAGGTATGCTGC 5 0.125 No Hit AAACAGACTCCGTGCAGCGGAGGCAATGGCTGAGGCTAGAGCTGCAGCTG 5 0.125 No Hit GTTTGAGGATGTACCATTTGACTTCCATGGGCTTCGAGGTGATATTCAAG 5 0.125 No Hit CACTGAGGCATCTCAGGTGCTGATTGAGCTCGAGGAGGCGAAGAAAGCTT 5 0.125 No Hit CTTCTACCCCCTTCCCTTTCTCTCTCTTTCTCTCTAAAAGAGTCAAAAGC 5 0.125 No Hit GTTTTTGTTTCCTGCTCTCTTCTAAGTGGAATTTGTCGAGAAGTCAGAAA 5 0.125 No Hit GTCTCAGTAACAAAGTTGGATTTAAGGGCTTTGCTGTGCCGAAAGAGGCA 5 0.125 No Hit GACTAATGAGGAGCTTACAAAGATTGTTGAAGAACATGGTGCTGTTGAAA 5 0.125 No Hit AGGGTCAGTTTCATGTTCGATTTGTCTTGAAGTGGTTGCTGACAATGGAG 5 0.125 No Hit CAGCTATTTATAAGGAGGAGAATAAGCAGATGGTGCCGTGTGTCTCCAAA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0125 0.0 0.0 0.0 0.0 64-65 0.075 0.0 0.0 0.0 0.0 66-67 0.2 0.0 0.0 0.0 0.0 68-69 0.2 0.0 0.0 0.0 0.0 70-71 0.2 0.0 0.0 0.0 0.0 72-73 0.2 0.0 0.0 0.0 0.0 74-75 0.2 0.0 0.0 0.0 0.0 76-77 0.2 0.0 0.0 0.0 0.0 78-79 0.2 0.0 0.0 0.0 0.0 80-81 0.225 0.0 0.0 0.0 0.0 82-83 0.225 0.0 0.0 0.0 0.0 84-85 0.2625 0.0 0.0 0.0 0.0 86-87 0.3 0.0 0.0 0.0 0.0 88-89 0.55 0.0 0.0 0.0 0.0 90-91 0.575 0.0 0.0 0.0 0.0 92-93 0.6375 0.0 0.0 0.0 0.0 94-95 0.7 0.0 0.0 0.0 0.0 96-97 0.825 0.0 0.0 0.0 0.0 98-99 1.0625 0.0 0.0 0.0 0.0 100-101 1.35 0.0 0.0 0.0 0.0 102-103 1.5875 0.0 0.0 0.0 0.0 104-105 1.775 0.0 0.0 0.0 0.0 106-107 2.0625 0.0 0.0 0.0 0.0 108-109 2.2874999999999996 0.0 0.0 0.0 0.0 110-111 2.4749999999999996 0.0 0.0 0.0 0.0 112-113 2.7125 0.0 0.0 0.0 0.0 114-115 3.2625 0.0 0.0 0.0 0.0 116-117 3.6125 0.0 0.0 0.0 0.0 118-119 4.05 0.0 0.0 0.0 0.0 120-121 4.3375 0.0 0.0 0.0 0.0 122-123 4.762499999999999 0.0 0.0 0.0 0.0 124-125 5.125 0.0 0.0 0.0 0.0 126-127 5.3875 0.0 0.0 0.0 0.0 128-129 5.8125 0.0 0.0 0.0 0.0 130-131 6.25 0.0 0.0 0.0 0.0 132-133 6.65 0.0 0.0 0.0 0.0 134-135 7.012499999999999 0.0 0.0 0.0 0.0 136-137 7.512499999999999 0.0 0.0 0.0 0.0 138-139 8.2375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGATCG 10 0.006830828 145.0 9 >>END_MODULE Read 1012277 spots for SRR13695422.sra Written 1012277 spots for SRR13695422.sra Read 1012277 spots for SRR13695422.sra Written 1012277 spots for SRR13695422.sra Read 1012277 spots for SRR13695422.sra Written 1012277 spots for SRR13695422.sra Read 1012277 spots for SRR13695422.sra Written 1012277 spots for SRR13695422.sra Read 1012277 spots for SRR13695422.sra Written 1012277 spots for SRR13695422.sra Read 1012277 spots for SRR13695422.sra Written 1012277 spots for SRR13695422.sra Read 1012277 spots for SRR13695422.sra Written 1012277 spots for SRR13695422.sra Read 1012277 spots for SRR13695422.sra Written 1012277 spots for SRR13695422.sra Read 1012277 spots for SRR13695422.sra Written 1012277 spots for SRR13695422.sra Read 1012277 spots for SRR13695422.sra Written 1012277 spots for SRR13695422.sra Read 1012277 spots for SRR13695422.sra Written 1012277 spots for SRR13695422.sra Read 1012277 spots for SRR13695422.sra Written 1012277 spots for SRR13695422.sra Read 1012288 spots for SRR13695422.sra Written 1012288 spots for SRR13695422.sra Read 1012277 spots for SRR13695422.sra Written 1012277 spots for SRR13695422.sra Read 1012277 spots for SRR13695422.sra Written 1012277 spots for SRR13695422.sra Read 1012277 spots for SRR13695422.sra Written 1012277 spots for SRR13695422.sra Read 1012277 spots for SRR13695422.sra Written 1012277 spots for SRR13695422.sra Read 1012277 spots for SRR13695422.sra Written 1012277 spots for SRR13695422.sra Read 1012277 spots for SRR13695422.sra Written 1012277 spots for SRR13695422.sra Read 1012277 spots for SRR13695422.sra Written 1012277 spots for SRR13695422.sra SRR ids: ['SRR13695422.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_huvcy38r SRR13695422.sra spots: 20245551 blocks: [[1, 1012277], [1012278, 2024554], [2024555, 3036831], [3036832, 4049108], [4049109, 5061385], [5061386, 6073662], [6073663, 7085939], [7085940, 8098216], [8098217, 9110493], [9110494, 10122770], [10122771, 11135047], [11135048, 12147324], [12147325, 13159601], [13159602, 14171878], [14171879, 15184155], [15184156, 16196432], [16196433, 17208709], [17208710, 18220986], [18220987, 19233263], [19233264, 20245551]] SRR13695422 file size 6858623 SRR13695422 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695422 SRR13695422_1.fastq SRR13695422_2.fastq Input file: SRR13695422_1.fastq Paired file: SRR13695422_2.fastq trimmed: SRR13695422-trimmed-pair1.fastq, SRR13695422-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 01:30:40 2025 >> started Wed Feb 12 01:31:04 2025 >> done (24.690s) 20245551 read pairs processed; of these: 152 ( 0.00%) short read pairs filtered out after trimming by size control 1071 ( 0.01%) empty read pairs filtered out after trimming by size control 20244328 (99.99%) read pairs available; of these: 2282441 (11.27%) trimmed read pairs available after processing 17961887 (88.73%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 2 0.00% 20 0 0.00% 21 1 0.00% 22 2 0.00% 23 1 0.00% 24 3 0.00% 25 0 0.00% 26 0 0.00% 27 3 0.00% 28 4 0.00% 29 3 0.00% 30 4 0.00% 31 3 0.00% 32 5 0.00% 33 6 0.00% 34 11 0.00% 35 7 0.00% 36 17 0.00% 37 9 0.00% 38 13 0.00% 39 21 0.00% 40 27 0.00% 41 25 0.00% 42 23 0.00% 43 30 0.00% 44 31 0.00% 45 31 0.00% 46 37 0.00% 47 45 0.00% 48 56 0.00% 49 67 0.00% 50 76 0.00% 51 58 0.00% 52 82 0.00% 53 118 0.00% 54 155 0.00% 55 115 0.00% 56 180 0.00% 57 185 0.00% 58 226 0.00% 59 242 0.00% 60 297 0.00% 61 371 0.00% 62 339 0.00% 63 397 0.00% 64 482 0.00% 65 534 0.00% 66 617 0.00% 67 674 0.00% 68 783 0.00% 69 924 0.00% 70 1034 0.01% 71 1158 0.01% 72 1392 0.01% 73 1658 0.01% 74 1849 0.01% 75 1996 0.01% 76 2150 0.01% 77 2406 0.01% 78 2797 0.01% 79 2948 0.01% 80 3190 0.02% 81 3632 0.02% 82 4252 0.02% 83 4754 0.02% 84 5332 0.03% 85 5926 0.03% 86 6442 0.03% 87 6825 0.03% 88 7474 0.04% 89 7935 0.04% 90 8451 0.04% 91 9020 0.04% 92 9996 0.05% 93 10843 0.05% 94 11490 0.06% 95 12702 0.06% 96 13435 0.07% 97 13982 0.07% 98 14815 0.07% 99 15143 0.07% 100 16266 0.08% 101 16963 0.08% 102 18006 0.09% 103 18860 0.09% 104 19817 0.10% 105 21235 0.10% 106 21895 0.11% 107 22921 0.11% 108 23458 0.12% 109 24685 0.12% 110 24860 0.12% 111 26537 0.13% 112 27033 0.13% 113 28102 0.14% 114 28642 0.14% 115 30235 0.15% 116 31008 0.15% 117 32559 0.16% 118 33739 0.17% 119 34372 0.17% 120 35819 0.18% 121 37004 0.18% 122 37490 0.19% 123 38289 0.19% 124 40192 0.20% 125 40142 0.20% 126 42768 0.21% 127 43508 0.21% 128 44239 0.22% 129 44340 0.22% 130 46419 0.23% 131 45996 0.23% 132 47928 0.24% 133 48728 0.24% 134 49191 0.24% 135 50709 0.25% 136 51902 0.26% 137 53295 0.26% 138 54304 0.27% 139 56578 0.28% 140 56088 0.28% 141 57443 0.28% 142 57993 0.29% 143 57477 0.28% 144 60293 0.30% 145 61128 0.30% 146 61170 0.30% 147 62685 0.31% 148 64495 0.32% 149 65112 0.32% 150 66153 0.33% 151 17961887 88.73% 20244328 reads passed initial QC criterion=sequence-density sequence-density=0.39 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=24 prefix-density=0.39 prefix-fanout=2.0 sequence=GTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGAGTATGAATGTGTTCTCTGT criterion=fanout-score sequence-density=0.01 sequence-density-rank=27 fanout-score=375.52 fanout-score-rank=1 prefix-density=0.27 prefix-fanout=15.6 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTC criterion=sequence-density sequence-density=0.53 sequence-density-rank=1 fanout-score=2.09 fanout-score-rank=28 prefix-density=0.54 prefix-fanout=2.1 sequence=TGTAAGAGATGGCTTCCTC criterion=fanout-score sequence-density=0.14 sequence-density-rank=20 fanout-score=33.43 fanout-score-rank=1 prefix-density=0.40 prefix-fanout=11.9 sequence=AAAGAAAAGAAAA SRR13695422 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 01:31:43 Started mapping on | Feb 12 01:31:43 Finished on | Feb 12 01:33:45 Mapping speed, Million of reads per hour | 597.37 Number of input reads | 20244328 Average input read length | 296 UNIQUE READS: Uniquely mapped reads number | 19245838 Uniquely mapped reads % | 95.07% Average mapped length | 295.15 Number of splices: Total | 19292786 Number of splices: Annotated (sjdb) | 18874450 Number of splices: GT/AG | 18899645 Number of splices: GC/AG | 319085 Number of splices: AT/AC | 11211 Number of splices: Non-canonical | 62845 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.03% Deletion average length | 2.91 Insertion rate per base | 0.02% Insertion average length | 2.33 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 432872 % of reads mapped to multiple loci | 2.14% Number of reads mapped to too many loci | 53500 % of reads mapped to too many loci | 0.26% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.44% % of reads unmapped: other | 0.09% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 565879 565879 565879 N_multimapping 432872 432872 432872 N_noFeature 722604 18750132 1026009 N_ambiguous 301340 1955 107723 UnstrandedReadsAssigned:18221894 PositiveStrandReadsAssigned:493751 NegativeStrandReadsAssigned:18112106 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695422 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695422-trimmed-pair1.fastq SRR13695422-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 20,244,328 reads, 18,112,917 reads pseudoaligned [quant] estimated average fragment length: 251.187 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,128 rounds 52401 SRR13695422.ke.tsv 34699 SRR13695422.se.tsv 87100 total ==> SRR13695422.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1767.81 391 10.862 Potri.005G024800.1.v4.1 1035 784.813 261 16.3322 Potri.004G059700.1.v4.1 961 710.992 0 0 Potri.007G009000.2.v4.1 1416 1165.81 0 0 Potri.003G141000.2.v4.1 2943 2692.81 1124.5 20.5079 Potri.016G087400.1.v4.1 270 84.264 830 483.734 Potri.015G069301.1.v4.1 564 323.407 0 0 Potri.010G195200.1.v4.1 1773 1522.81 57 1.83822 Potri.012G127500.1.v4.1 977 726.925 84 5.67492 ==> SRR13695422.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 270 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 296 Potri.001G212900.v4.1 2 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 6 SRR13695422 completed mapping pipeline successfully