Starting /dee2/code/volunteer_pipeline.sh SRR13695423 current disk space = 3049027313664 free memory = 1582324596 SRR13695423 SRAfilesize 70b759387b4c3abaf2b500d08a28a1c8 SRR13695423.sra SRR13695423.sra file validated SRR13695423 is paired end SRR13695423 is conventional basespace SRR13695423 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695423_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.62 37.0 37.0 37.0 37.0 37.0 2 36.39775 37.0 37.0 37.0 37.0 37.0 3 36.503 37.0 37.0 37.0 37.0 37.0 4 36.557 37.0 37.0 37.0 37.0 37.0 5 36.5345 37.0 37.0 37.0 37.0 37.0 6 36.5695 37.0 37.0 37.0 37.0 37.0 7 36.4885 37.0 37.0 37.0 37.0 37.0 8 36.606 37.0 37.0 37.0 37.0 37.0 9 36.561 37.0 37.0 37.0 37.0 37.0 10-14 36.563900000000004 37.0 37.0 37.0 37.0 37.0 15-19 36.5201 37.0 37.0 37.0 37.0 37.0 20-24 36.4661 37.0 37.0 37.0 37.0 37.0 25-29 36.4499 37.0 37.0 37.0 37.0 37.0 30-34 36.4217 37.0 37.0 37.0 37.0 37.0 35-39 36.394000000000005 37.0 37.0 37.0 37.0 37.0 40-44 36.4149 37.0 37.0 37.0 37.0 37.0 45-49 36.320299999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.3377 37.0 37.0 37.0 37.0 37.0 55-59 36.319100000000006 37.0 37.0 37.0 37.0 37.0 60-64 36.2981 37.0 37.0 37.0 37.0 37.0 65-69 36.277100000000004 37.0 37.0 37.0 37.0 37.0 70-74 36.2312 37.0 37.0 37.0 37.0 37.0 75-79 36.2673 37.0 37.0 37.0 37.0 37.0 80-84 36.2023 37.0 37.0 37.0 37.0 37.0 85-89 36.1927 37.0 37.0 37.0 37.0 37.0 90-94 36.112399999999994 37.0 37.0 37.0 37.0 37.0 95-99 36.1126 37.0 37.0 37.0 37.0 37.0 100-104 36.11429999999999 37.0 37.0 37.0 37.0 37.0 105-109 36.060500000000005 37.0 37.0 37.0 37.0 37.0 110-114 36.0613 37.0 37.0 37.0 37.0 37.0 115-119 36.0545 37.0 37.0 37.0 37.0 37.0 120-124 35.9474 37.0 37.0 37.0 37.0 37.0 125-129 35.9631 37.0 37.0 37.0 37.0 37.0 130-134 35.96849999999999 37.0 37.0 37.0 37.0 37.0 135-139 35.847 37.0 37.0 37.0 37.0 37.0 140-144 35.774 37.0 37.0 37.0 37.0 37.0 145-149 35.620200000000004 37.0 37.0 37.0 37.0 37.0 150-151 35.522999999999996 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 1.0 21 1.0 22 4.0 23 3.0 24 4.0 25 2.0 26 8.0 27 11.0 28 15.0 29 25.0 30 32.0 31 47.0 32 45.0 33 66.0 34 85.0 35 284.0 36 2971.0 37 395.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.624999999999996 13.625000000000002 11.85 42.9 2 21.419613744670176 15.174316528718334 32.45548031101079 30.950589415600703 3 18.224999999999998 17.05 24.675 40.050000000000004 4 21.95 23.75 23.200000000000003 31.1 5 22.525000000000002 28.675 25.1 23.7 6 20.974999999999998 34.599999999999994 23.3 21.125 7 16.425 29.7 38.5 15.375 8 18.6 27.474999999999998 29.099999999999998 24.825 9 17.4 26.6 34.1 21.9 10-14 19.580000000000002 30.845 27.310000000000002 22.264999999999997 15-19 19.93 28.325 28.225 23.52 20-24 19.115 29.709999999999997 27.365000000000002 23.810000000000002 25-29 19.16 29.395 27.315 24.13 30-34 20.23 28.910000000000004 27.575 23.285 35-39 20.135 28.854999999999997 27.76 23.25 40-44 20.294999999999998 28.810000000000002 26.919999999999998 23.974999999999998 45-49 20.255000000000003 28.4 27.61 23.735 50-54 20.465 29.43 26.640000000000004 23.465 55-59 19.72 28.64 27.950000000000003 23.69 60-64 19.505 29.265 26.790000000000003 24.44 65-69 20.165 29.195 27.05 23.59 70-74 20.225 28.33 27.994999999999997 23.45 75-79 20.405 28.389999999999997 27.83 23.375 80-84 20.16 28.27 27.36 24.21 85-89 21.04 28.065 27.315 23.580000000000002 90-94 20.0 28.07 26.965 24.965 95-99 20.275000000000002 28.015 27.54 24.169999999999998 100-104 20.955 28.775000000000002 26.810000000000002 23.46 105-109 20.61 27.67 27.450000000000003 24.27 110-114 21.029999999999998 29.23 25.985000000000003 23.755000000000003 115-119 21.385 27.339999999999996 27.61 23.665 120-124 22.005 28.74 25.77 23.485 125-129 21.044999999999998 28.610000000000003 26.484999999999996 23.86 130-134 20.885 28.67 26.05 24.395 135-139 21.54 28.345 26.05 24.065 140-144 21.07 27.875 27.195000000000004 23.86 145-149 21.075 27.779999999999998 26.105 25.040000000000003 150-151 20.6625 28.000000000000004 26.1625 25.174999999999997 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.5 4 0.5 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.0 20 3.5 21 2.5 22 0.0 23 0.0 24 0.0 25 0.5 26 4.5 27 8.0 28 6.0 29 9.0 30 17.5 31 32.5 32 43.5 33 54.5 34 70.0 35 87.5 36 110.5 37 131.5 38 140.5 39 147.0 40 170.5 41 197.5 42 213.5 43 212.5 44 241.5 45 268.0 46 248.5 47 226.0 48 195.0 49 179.0 50 178.5 51 161.5 52 146.0 53 123.0 54 85.0 55 68.5 56 54.5 57 38.0 58 34.0 59 27.5 60 17.5 61 12.5 62 9.5 63 3.0 64 3.0 65 5.0 66 4.5 67 1.5 68 0.0 69 0.0 70 0.0 71 0.0 72 1.0 73 1.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.325 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.025 #Duplication Level Percentage of deduplicated Percentage of total 1 72.0456979650125 50.449999999999996 2 18.457693680828275 25.85 3 6.069260978222064 12.75 4 2.3563013209568013 6.6000000000000005 5 0.5355230274901821 1.875 6 0.32131381649410923 1.35 7 0.14280614066404856 0.7000000000000001 8 0.03570153516601214 0.2 9 0.03570153516601214 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAAT 9 0.22499999999999998 No Hit GTAGTACCGGTACAAACATCCTCATAAGTTGGATTATGCAATAAGCCCTG 8 0.2 No Hit AAGGAATCAATATTTCATCCTTCTTGATAGAATAATCTGCCATATTTTTG 7 0.17500000000000002 No Hit CTTTGAGCCTAAAGTTTACAGCATGAGACAAAAGTCTCAAGAGTTTACGC 7 0.17500000000000002 No Hit GCTACATATTGTGTCAAGTGCCAATATTTTGAACTTCCATCCATCTATGA 7 0.17500000000000002 No Hit GCCCACACTGCTGGCCAGCGGCACATGAGTTGGAGTTGTAGTCGTAGTAA 7 0.17500000000000002 No Hit GCAGTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAA 6 0.15 No Hit ATCACTACGACTTCGGATACATTGCACTCACGAAGACGAGACCTCATAGA 6 0.15 No Hit TCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGC 6 0.15 No Hit TAAATACACTATTGTTACTGTTTTTCTTGTTGAAAATTACATTGTTGGTG 6 0.15 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATCGACATCTCGTAT 6 0.15 TruSeq Adapter, Index 2 (97% over 37bp) AGCTCGATCAATGGCGAGAATCAGAGGAAGCAGAGGACGGCTTGTTGTGA 6 0.15 No Hit CGCTCGTGTAAAACTGAACTTACAAAATGAGGTCACAATATTTAACACCA 6 0.15 No Hit CTGCCACAACAGACGCCGTCTCCCACCTATCGGAGCTCTCCACCAGTCTC 6 0.15 No Hit GCTCCTACCTGTGTCAGCAGCTGCTTCACCCTATTGCAATAGCCACAGTA 6 0.15 No Hit GGGGTCAGTTTACACCACAGATCTAAAACAAATTAAACACCCAATAGCAA 5 0.125 No Hit TGCTACTGCAGGCTACTAGTTTAGCAAAGAGCTGCTACATTATACGCCTA 5 0.125 No Hit GCCATCTTTATCAGCACCCACAGTTTTGTCCACTAATTTTCCATCTTTCA 5 0.125 No Hit ACGGCAAGCAAAGGAGTGCAAGGAGCTAGGGATTGCCATTTAGCAAAACA 5 0.125 No Hit CTTCATCCTTTACTTCATGAGATCGCACAACTAAATCTAGGTTATTGTCC 5 0.125 No Hit CCACTGTTTATTCAATGACCAAAAAGGCAAGCACCAGCTTTGTTTCAGGA 5 0.125 No Hit GGCACTTTAAACCCGGTTAATTTCTTTTCCCTACAGTAGTCTCTGAGAAC 5 0.125 No Hit ACCAAATTAAGTAGCTGAAACCAGATTGAGACAGAGACATGCCAAGTCAA 5 0.125 No Hit CGGCTGTCGAGTTGTACGGCCGTTCAGCCACGAGTCACGGGGTCTAACGC 5 0.125 No Hit GTCAAGGTTGTAAGAATCTGCAAGACCTTCCACGAGGTCGAATTCGGGTT 5 0.125 No Hit GTCAACACCAACGCCTGAGACCCACTTCCTCACCTCATCGTCATAAACTC 5 0.125 No Hit CTTGATCAAGAATGTTTCTTTCCTTGTATATCTTTAGCGTTTCAATGGCA 5 0.125 No Hit CTCACAGGTATGCGTATCTCCGCTCCGAGCTCAAGAATGTTTCTGGCTGC 5 0.125 No Hit GAGGAAACAATGCATACTGTTCCGTATCGCATAGCATGCAACTGAATGAG 5 0.125 No Hit CTTCACGAATAGCATTTATACCAGCCTCTGCTATTACAGATGGAGTATCA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.225 0.0 0.0 0.0 0.0 76-77 0.325 0.0 0.0 0.0 0.0 78-79 0.3625 0.0 0.0 0.0 0.0 80-81 0.4625 0.0 0.0 0.0 0.0 82-83 0.5 0.0 0.0 0.0 0.0 84-85 0.5125 0.0 0.0 0.0 0.0 86-87 0.5375000000000001 0.0 0.0 0.0 0.0 88-89 0.575 0.0 0.0 0.0 0.0 90-91 0.675 0.0 0.0 0.0 0.0 92-93 0.7625 0.0 0.0 0.0 0.0 94-95 0.8875 0.0 0.0 0.0 0.0 96-97 1.025 0.0 0.0 0.0 0.0 98-99 1.4249999999999998 0.0 0.0 0.0 0.0 100-101 1.95 0.0 0.0 0.0 0.0 102-103 2.2625 0.0 0.0 0.0 0.0 104-105 2.55 0.0 0.0 0.0 0.0 106-107 2.925 0.0 0.0 0.0 0.0 108-109 3.2875 0.0 0.0 0.0 0.0 110-111 3.5125 0.0 0.0 0.0 0.0 112-113 4.012499999999999 0.0 0.0 0.0 0.0 114-115 4.4 0.0 0.0 0.0 0.0 116-117 4.9 0.0 0.0 0.0 0.0 118-119 5.2125 0.0 0.0 0.0 0.0 120-121 5.762499999999999 0.0 0.0 0.0 0.0 122-123 6.5375 0.0 0.0 0.0 0.0 124-125 7.0875 0.0 0.0 0.0 0.0 126-127 7.7 0.0 0.0 0.0 0.0 128-129 8.2375 0.0 0.0 0.0 0.0 130-131 9.0 0.0 0.0 0.0 0.0 132-133 9.7 0.0 0.0 0.0 0.0 134-135 10.125 0.0 0.0 0.0 0.0 136-137 10.8125 0.0 0.0 0.0 0.0 138-139 11.3625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATGACA 10 0.006830828 145.0 4 AACAACT 10 0.006830828 145.0 5 TGACTTC 10 0.006830828 145.0 145 ACAGACG 10 0.006830828 145.0 9 AACTGTG 10 0.006830828 145.0 8 TGACTGA 10 0.006830828 145.0 8 CCTCGCA 10 0.006830828 145.0 145 TGACAGC 10 0.006830828 145.0 6 CCATGAC 10 0.006830828 145.0 3 CCCATGA 10 0.006830828 145.0 2 GTGACTG 10 0.006830828 145.0 7 GACTGAC 10 0.006830828 145.0 9 GCCACAA 10 0.006830828 145.0 3 TTTGGTG 10 0.006830828 145.0 3 CTTTGGT 10 0.006830828 145.0 2 TGAATGA 10 0.006830828 145.0 7 TTTTTTT 200 0.0036047986 10.875 4 >>END_MODULE SRR13695423 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695423_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.17425 37.0 37.0 37.0 37.0 37.0 2 36.2615 37.0 37.0 37.0 37.0 37.0 3 36.284 37.0 37.0 37.0 37.0 37.0 4 36.333 37.0 37.0 37.0 37.0 37.0 5 36.363 37.0 37.0 37.0 37.0 37.0 6 36.4155 37.0 37.0 37.0 37.0 37.0 7 36.3685 37.0 37.0 37.0 37.0 37.0 8 36.3895 37.0 37.0 37.0 37.0 37.0 9 36.226 37.0 37.0 37.0 37.0 37.0 10-14 36.324200000000005 37.0 37.0 37.0 37.0 37.0 15-19 36.2999 37.0 37.0 37.0 37.0 37.0 20-24 36.2618 37.0 37.0 37.0 37.0 37.0 25-29 36.207049999999995 37.0 37.0 37.0 37.0 37.0 30-34 36.1736 37.0 37.0 37.0 37.0 37.0 35-39 36.16215 37.0 37.0 37.0 37.0 37.0 40-44 36.126850000000005 37.0 37.0 37.0 37.0 37.0 45-49 36.15515 37.0 37.0 37.0 37.0 37.0 50-54 36.08895 37.0 37.0 37.0 37.0 37.0 55-59 36.08585 37.0 37.0 37.0 37.0 37.0 60-64 36.06665 37.0 37.0 37.0 37.0 37.0 65-69 36.048649999999995 37.0 37.0 37.0 37.0 37.0 70-74 35.97235 37.0 37.0 37.0 37.0 37.0 75-79 36.00195 37.0 37.0 37.0 37.0 37.0 80-84 36.0058 37.0 37.0 37.0 37.0 37.0 85-89 35.95305 37.0 37.0 37.0 37.0 37.0 90-94 35.9559 37.0 37.0 37.0 37.0 37.0 95-99 35.9498 37.0 37.0 37.0 37.0 37.0 100-104 35.932849999999995 37.0 37.0 37.0 37.0 37.0 105-109 35.88585 37.0 37.0 37.0 37.0 37.0 110-114 35.859750000000005 37.0 37.0 37.0 37.0 37.0 115-119 35.747949999999996 37.0 37.0 37.0 37.0 37.0 120-124 35.69985 37.0 37.0 37.0 37.0 37.0 125-129 35.7012 37.0 37.0 37.0 37.0 37.0 130-134 35.68445 37.0 37.0 37.0 37.0 37.0 135-139 35.6956 37.0 37.0 37.0 37.0 37.0 140-144 35.40735 37.0 37.0 37.0 34.6 37.0 145-149 35.222449999999995 37.0 37.0 37.0 34.6 37.0 150-151 34.951625 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 3.0 13 4.0 14 4.0 15 0.0 16 1.0 17 3.0 18 2.0 19 1.0 20 2.0 21 2.0 22 3.0 23 2.0 24 8.0 25 5.0 26 3.0 27 17.0 28 14.0 29 14.0 30 23.0 31 34.0 32 55.0 33 71.0 34 134.0 35 462.0 36 2904.0 37 227.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 31.878605467770253 21.143717080511664 17.03034863305744 29.947328818660644 2 30.425 25.650000000000002 25.900000000000002 18.025 3 23.375 28.249999999999996 28.125 20.25 4 23.225 33.650000000000006 23.7 19.425 5 25.974999999999998 35.275 21.65 17.1 6 23.474999999999998 37.75 20.875 17.9 7 22.275 22.475 36.15 19.1 8 24.15 24.675 26.575 24.6 9 22.075 25.825 30.8 21.3 10-14 24.39 29.15 25.174999999999997 21.285 15-19 24.04 27.515 26.68 21.765 20-24 24.307153576788394 28.61430715357679 26.88844422211106 20.190095047523762 25-29 24.028021015761823 27.955966975231423 27.280460345258945 20.735551663747813 30-34 23.401380966676673 28.24477133993796 27.008906234364055 21.344941459021317 35-39 23.532943118715295 28.240532292761017 27.054880184101254 21.171644404422434 40-44 23.80547355781258 27.898133786961527 27.662980937609444 20.63341171761645 45-49 24.235753239605742 27.838094761595038 27.15765247410817 20.76849952469105 50-54 23.778077942868578 27.930361698934412 26.379508729801394 21.912051628395616 55-59 24.07305479109332 27.795846885163872 27.66574931198399 20.465349011758818 60-64 23.668017409575267 27.365050777927863 27.75026264445445 21.216669168042422 65-69 23.89836442754964 27.199519831941178 28.680038013304653 20.22207772720452 70-74 24.13309982486865 27.455591693770327 27.31548661496122 21.0958218663998 75-79 24.573430072554416 27.615711783837877 27.970978233675257 19.83987990993245 80-84 24.842421210605302 27.888944472236116 27.298649324662332 19.969984992496247 85-89 23.577683262446836 27.015261446084565 28.41130848136102 20.99574681010758 90-94 24.369621773063837 27.656593956373825 27.296377826696016 20.67740644386632 95-99 23.99439663798279 28.99239543726236 26.260756453872325 20.75245147088253 100-104 24.928696522391792 28.45634225669252 26.114585939454592 20.500375281461096 105-109 24.513385038779084 27.705779334500875 27.760820615461597 20.020015011258444 110-114 25.203862124168293 26.89479213567462 27.850317674721097 20.05102806543599 115-119 24.563422566925194 28.526394796097073 26.53490117588191 20.37528146109582 120-124 25.74430823117338 27.9009256942707 27.47560670502877 18.879159369527144 125-129 25.953167217051938 27.749424597218052 26.97388171720204 19.32352646852797 130-134 24.82109793324326 28.509232847920735 26.72771856077666 19.94195065805935 135-139 25.65552441953563 27.802241793434746 27.13170536429143 19.410528422738192 140-144 25.739156536094853 27.965380959527742 26.31447296012807 19.980989544249336 145-149 26.730047535651742 27.8558919189392 26.174630973229924 19.239429572179134 150-151 26.310521706493184 28.737645439759792 25.534842987614166 19.416989866132866 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2.0 1 1.0 2 0.0 3 0.0 4 0.0 5 0.5 6 1.5 7 1.0 8 0.0 9 0.5 10 0.5 11 1.5 12 1.5 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 1.0 19 1.0 20 1.0 21 2.5 22 2.0 23 0.0 24 0.5 25 1.5 26 2.0 27 3.0 28 4.0 29 5.5 30 10.0 31 13.0 32 16.0 33 21.0 34 28.0 35 37.5 36 66.5 37 102.5 38 133.5 39 163.0 40 203.5 41 227.0 42 219.0 43 235.0 44 261.5 45 278.5 46 286.0 47 265.0 48 231.0 49 200.0 50 179.5 51 159.5 52 134.5 53 116.0 54 93.0 55 69.5 56 56.5 57 41.5 58 32.5 59 33.5 60 19.0 61 6.0 62 4.0 63 3.5 64 4.0 65 3.0 66 0.5 67 0.5 68 0.5 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.5 84 0.5 85 0.5 86 0.5 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.5 93 1.5 94 2.0 95 1.5 96 1.0 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.325 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.05 25-29 0.075 30-34 0.06999999999999999 35-39 0.055 40-44 0.065 45-49 0.065 50-54 0.055 55-59 0.075 60-64 0.055 65-69 0.034999999999999996 70-74 0.075 75-79 0.075 80-84 0.05 85-89 0.075 90-94 0.06 95-99 0.06 100-104 0.075 105-109 0.075 110-114 0.055 115-119 0.075 120-124 0.075 125-129 0.06999999999999999 130-134 0.08499999999999999 135-139 0.08 140-144 0.055 145-149 0.075 150-151 0.08750000000000001 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.525 #Duplication Level Percentage of deduplicated Percentage of total 1 72.66926621765332 51.24999999999999 2 18.04324707550514 25.45 3 6.20347394540943 13.125 4 1.9851116625310175 5.6000000000000005 5 0.5671747607231479 2.0 6 0.28358738036157394 1.2 7 0.1772421127259837 0.8750000000000001 8 0.03544842254519674 0.2 9 0.0 0.0 >10 0.03544842254519674 0.3 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTCAATATAAGCCTTGGTAGGGATAGATAGCCACCTATATAGTATAGCT 12 0.3 No Hit GCATTTCAAAGAGTCCCTGGTGTTACTAAAACTGAAGTGGGTTATACTCA 8 0.2 No Hit AGGGAAGAACGATTAGCCATGGGACCTGGGGCAGAAAAGCCAGTAGCTGC 7 0.17500000000000002 No Hit CCACAGTGCAAGCCTATGCACAAAATTATGCTAACCAACGTGCCGGCGAT 7 0.17500000000000002 No Hit AGAACTTTCAAGGATTGCTTATGTGGAAGCATCTGGGTTCAATAACACTG 7 0.17500000000000002 No Hit GATATCTGGACAGACTAGTGAGTACATTGGAAGCAAAGAAGCATCATGAG 7 0.17500000000000002 No Hit ACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGTCAGGG 7 0.17500000000000002 No Hit ATTAAAGAGGCTGGTGGTGCTGTTGTACTTACAGCCTAGGTTATGTTCCT 6 0.15 No Hit CAGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 6 0.15 No Hit CATAGACTAGCCTGCCGGTCAATAACTGATGACGCGGAGTCAACCTGATA 6 0.15 No Hit TTTATCATCAGCAAACTGCAATATCAGGCATTGGATTCACTGCCTCAGGT 6 0.15 No Hit GGATAGCATACTCCTTTCGAGTTGTCTAGGCAAGTTCTTTTCTAGATTGA 6 0.15 No Hit TGGGATTGTTTACCACCCAGATGGGTTCTTGATTGTCATCCATACCTTTA 6 0.15 No Hit TGTTAAGTTCTTCAATCAAGATGAGCAAGCAAGAACTTGATGCTGCGCTT 6 0.15 No Hit AGCTGACATTGACCCGATTTATCCAGTTGAAGGAGGTGGAGGAAGGTGCT 6 0.15 No Hit CTGAATTGCCCTCAGCTCATGCCATACTAGATGTTTGGAAGGAAAGCAGG 5 0.125 No Hit AGAGAACACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 5 0.125 No Hit GCTTGAGGAGGCAAAGAAAGCTTACCCTAACGCCTTTATCCGTATAATCG 5 0.125 No Hit TATTCTTGCTGCTGCCATTCCAATGCTGGTTAAAAACAATGTGCAGATAA 5 0.125 No Hit CAGACGTTTTTCTGTGCTTCATATTATCGAGAGAGAAAATGGCCGAAGAA 5 0.125 No Hit GTGTCATTCAGCTTTTATATCCCCTATTCTCTTCGTGAAGATGTCTTGCT 5 0.125 No Hit AGAACACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATC 5 0.125 No Hit CTGAGAACCATTTAGTTTTGGTGAACCTGAAGAACAAGGGTATTGATGGC 5 0.125 No Hit GTTCTTTACATCAATCCATATTGGCGACGCATGTGGTTGTACTTCATCGA 5 0.125 No Hit TCCAGAAGCTCGTTTGACAGAGATGGTTGTTGCTTGCATTAAATTAAGAC 5 0.125 No Hit TGGACGGGCACGCTCATATCAGGCTATATTTGGTCCGGGTTATTATCGTC 5 0.125 No Hit ATTGATGTGTGAATTGCTATGGAGTGATCCACAGCCATTCCCTGGAAGAG 5 0.125 No Hit CTAAAATTTACAAGAGGCAGAAATAGAGAAATGCAAACAGAAGTTGGATG 5 0.125 No Hit CAAACCAGACCTTGTATCAGTAGCAAAGGCCCTTTCCTCTGGTTACTTGC 5 0.125 No Hit GGGATCAAAGAAAAGGTAGATGCAAAGGCAATTGAGAATGGCAGCAACGC 5 0.125 No Hit ATTTGTTTTTGCTCAGGTGAAGCATGGACTTGGCATAGAGGAAATTGTGA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.225 0.0 0.0 0.0 0.0 76-77 0.325 0.0 0.0 0.0 0.0 78-79 0.3625 0.0 0.0 0.0 0.0 80-81 0.4625 0.0 0.0 0.0 0.0 82-83 0.5 0.0 0.0 0.0 0.0 84-85 0.5125 0.0 0.0 0.0 0.0 86-87 0.5375000000000001 0.0 0.0 0.0 0.0 88-89 0.575 0.0 0.0 0.0 0.0 90-91 0.675 0.0 0.0 0.0 0.0 92-93 0.7875000000000001 0.0 0.0 0.0 0.0 94-95 0.9125 0.0 0.0 0.0 0.0 96-97 1.0625 0.0 0.0 0.0 0.0 98-99 1.475 0.0 0.0 0.0 0.0 100-101 2.0 0.0 0.0 0.0 0.0 102-103 2.3125 0.0 0.0 0.0 0.0 104-105 2.5999999999999996 0.0 0.0 0.0 0.0 106-107 2.975 0.0 0.0 0.0 0.0 108-109 3.3375 0.0 0.0 0.0 0.0 110-111 3.5625 0.0 0.0 0.0 0.0 112-113 4.0625 0.0 0.0 0.0 0.0 114-115 4.449999999999999 0.0 0.0 0.0 0.0 116-117 5.0 0.0 0.0 0.0 0.0 118-119 5.3125 0.0 0.0 0.0 0.0 120-121 5.862500000000001 0.0 0.0 0.0 0.0 122-123 6.6375 0.0 0.0 0.0 0.0 124-125 7.1875 0.0 0.0 0.0 0.0 126-127 7.825 0.0 0.0 0.0 0.0 128-129 8.325 0.0 0.0 0.0 0.0 130-131 9.075 0.0 0.0 0.0 0.0 132-133 9.8 0.0 0.0 0.0 0.0 134-135 10.225000000000001 0.0 0.0 0.0 0.0 136-137 10.912500000000001 0.0 0.0 0.0 0.0 138-139 11.4625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTTTA 10 0.006830828 145.0 6 AACCGCA 10 0.006830828 145.0 145 AAGAGTC 10 0.006830828 145.0 9 TGATTCT 10 0.006830828 145.0 6 CATTTTA 10 0.006830828 145.0 145 GAAGAGT 10 0.006830828 145.0 8 GATTGTT 10 0.006830828 145.0 4 AGAACTC 10 0.006830828 145.0 145 TTGATTC 10 0.006830828 145.0 5 GATTCTG 10 0.006830828 145.0 7 ATTGATT 10 0.006830828 145.0 4 ATTCTGA 10 0.006830828 145.0 8 TTTACCA 10 0.006830828 145.0 9 >>END_MODULE Read 862114 spots for SRR13695423.sra Written 862114 spots for SRR13695423.sra Read 862114 spots for SRR13695423.sra Written 862114 spots for SRR13695423.sra Read 862114 spots for SRR13695423.sra Written 862114 spots for SRR13695423.sra Read 862114 spots for SRR13695423.sra Written 862114 spots for SRR13695423.sra Read 862114 spots for SRR13695423.sra Written 862114 spots for SRR13695423.sra Read 862114 spots for SRR13695423.sra Written 862114 spots for SRR13695423.sra Read 862114 spots for SRR13695423.sra Written 862114 spots for SRR13695423.sra Read 862114 spots for SRR13695423.sra Written 862114 spots for SRR13695423.sra Read 862114 spots for SRR13695423.sra Written 862114 spots for SRR13695423.sra Read 862114 spots for SRR13695423.sra Written 862114 spots for SRR13695423.sra Read 862114 spots for SRR13695423.sra Written 862114 spots for SRR13695423.sra Read 862114 spots for SRR13695423.sra Written 862114 spots for SRR13695423.sra Read 862114 spots for SRR13695423.sra Written 862114 spots for SRR13695423.sra Read 862114 spots for SRR13695423.sra Written 862114 spots for SRR13695423.sra Read 862114 spots for SRR13695423.sra Written 862114 spots for SRR13695423.sra Read 862114 spots for SRR13695423.sra Written 862114 spots for SRR13695423.sra Read 862114 spots for SRR13695423.sra Written 862114 spots for SRR13695423.sra Read 862114 spots for SRR13695423.sra Written 862114 spots for SRR13695423.sra Read 862128 spots for SRR13695423.sra Written 862128 spots for SRR13695423.sra Read 862114 spots for SRR13695423.sra Written 862114 spots for SRR13695423.sra SRR ids: ['SRR13695423.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_asaljm5o SRR13695423.sra spots: 17242294 blocks: [[1, 862114], [862115, 1724228], [1724229, 2586342], [2586343, 3448456], [3448457, 4310570], [4310571, 5172684], [5172685, 6034798], [6034799, 6896912], [6896913, 7759026], [7759027, 8621140], [8621141, 9483254], [9483255, 10345368], [10345369, 11207482], [11207483, 12069596], [12069597, 12931710], [12931711, 13793824], [13793825, 14655938], [14655939, 15518052], [15518053, 16380166], [16380167, 17242294]] SRR13695423 file size 5837985 SRR13695423 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695423 SRR13695423_1.fastq SRR13695423_2.fastq Input file: SRR13695423_1.fastq Paired file: SRR13695423_2.fastq trimmed: SRR13695423-trimmed-pair1.fastq, SRR13695423-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 02:26:09 2025 >> started Wed Feb 12 02:26:28 2025 >> done (18.837s) 17242294 read pairs processed; of these: 132 ( 0.00%) short read pairs filtered out after trimming by size control 37614 ( 0.22%) empty read pairs filtered out after trimming by size control 17204548 (99.78%) read pairs available; of these: 2766005 (16.08%) trimmed read pairs available after processing 14438543 (83.92%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 3 0.00% 20 1 0.00% 21 1 0.00% 22 0 0.00% 23 1 0.00% 24 1 0.00% 25 2 0.00% 26 2 0.00% 27 6 0.00% 28 3 0.00% 29 2 0.00% 30 4 0.00% 31 8 0.00% 32 5 0.00% 33 6 0.00% 34 5 0.00% 35 8 0.00% 36 4 0.00% 37 12 0.00% 38 11 0.00% 39 14 0.00% 40 20 0.00% 41 23 0.00% 42 25 0.00% 43 33 0.00% 44 28 0.00% 45 39 0.00% 46 43 0.00% 47 54 0.00% 48 58 0.00% 49 84 0.00% 50 110 0.00% 51 105 0.00% 52 108 0.00% 53 120 0.00% 54 153 0.00% 55 167 0.00% 56 177 0.00% 57 211 0.00% 58 241 0.00% 59 297 0.00% 60 348 0.00% 61 364 0.00% 62 467 0.00% 63 596 0.00% 64 621 0.00% 65 711 0.00% 66 797 0.00% 67 880 0.01% 68 1006 0.01% 69 1131 0.01% 70 1280 0.01% 71 1543 0.01% 72 1778 0.01% 73 1950 0.01% 74 2418 0.01% 75 2747 0.02% 76 2878 0.02% 77 3123 0.02% 78 3669 0.02% 79 4141 0.02% 80 4445 0.03% 81 5033 0.03% 82 5841 0.03% 83 6335 0.04% 84 7063 0.04% 85 8071 0.05% 86 8392 0.05% 87 8984 0.05% 88 9978 0.06% 89 10443 0.06% 90 11216 0.07% 91 12073 0.07% 92 12661 0.07% 93 14180 0.08% 94 15340 0.09% 95 16101 0.09% 96 17154 0.10% 97 18342 0.11% 98 18726 0.11% 99 20197 0.12% 100 20933 0.12% 101 21438 0.12% 102 22803 0.13% 103 23961 0.14% 104 25630 0.15% 105 26738 0.16% 106 28221 0.16% 107 28835 0.17% 108 29974 0.17% 109 31609 0.18% 110 31701 0.18% 111 33046 0.19% 112 34604 0.20% 113 35051 0.20% 114 37023 0.22% 115 38836 0.23% 116 39772 0.23% 117 40930 0.24% 118 42622 0.25% 119 43157 0.25% 120 44095 0.26% 121 44985 0.26% 122 46077 0.27% 123 47115 0.27% 124 49473 0.29% 125 49776 0.29% 126 51451 0.30% 127 53121 0.31% 128 54015 0.31% 129 53721 0.31% 130 56000 0.33% 131 56132 0.33% 132 56286 0.33% 133 59423 0.35% 134 59755 0.35% 135 60394 0.35% 136 60977 0.35% 137 62041 0.36% 138 63952 0.37% 139 65327 0.38% 140 65700 0.38% 141 65761 0.38% 142 67288 0.39% 143 68116 0.40% 144 69307 0.40% 145 69760 0.41% 146 70811 0.41% 147 71627 0.42% 148 73566 0.43% 149 73317 0.43% 150 74533 0.43% 151 14438543 83.92% 17204548 reads passed initial QC criterion=sequence-density sequence-density=0.68 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=26 prefix-density=0.68 prefix-fanout=2.0 sequence=GTGTTGTCGAATCC criterion=fanout-score sequence-density=0.02 sequence-density-rank=27 fanout-score=31.20 fanout-score-rank=1 prefix-density=0.09 prefix-fanout=7.3 sequence=AAACAGAATATTTACTTTTAGCGCAAGTTTCAATTCATGAGCTCGTTACATCACAAGTTAACATTACAAGCCAATAGTCCCAGCACAGAAAAAACTCTTTTGTTGCTCACTTTCCGGGAACGAAGTTTGTGGCATATGCCCAGGCGTTGTTGTTTACTGGGTCAGAAAGGTGGTCAGCCAGGTTCTCCAGTGGTCCCTTTCCGGTCACAATGGCCTGGACAAAGAATCCGAACATTGAGAACATAGCCAACCT criterion=sequence-density sequence-density=0.68 sequence-density-rank=1 fanout-score=2.30 fanout-score-rank=18 prefix-density=0.71 prefix-fanout=2.2 sequence=CCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAATCATCATGTCTAGCACCTGCGACACCTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGAC criterion=fanout-score sequence-density=0.01 sequence-density-rank=33 fanout-score=56.04 fanout-score-rank=1 prefix-density=0.08 prefix-fanout=5.1 sequence=AAACCCAAAGCTCAAAAAAAATCTTAACTGCACCCGCTCATCCAGCAATGGCAGCAGCAACAATGGCCCTCTCCTCCCCTTCGCTAGCCGGAAAGGCGGTGAAGCTCAACCCCTCCTCCTCTGAGATCATGGGCAATGGCCGTGTCTCCATGAGGAAAACCACCAAGCCTGTTCCCTCCGGGAGCCCATGGTACGGACCAGACCGTGTTAAATACTTGGGCCCGTTCTCTGGTGAGCCCCCATCCTACTTGACTGGTGAGTTCCCTGGTGACTACGGCTGGGACACTGCTGGCCTCTCTGCTGACCCAGAGACCTTTGCCAAGAACCGTGAGCTTGAAGTCATCCATTCCAGGTGGGCCATGCTTGGAGCTCTTGGATGCGTCTTCCCCGAGCTCTTGTCCCGCAACGGTGTCAAGTTCGGCGAGGCTGTATGGTTCAAGGCTGGAGCCCAGATCTTCAGCGAGGGTGGACTTGACTACTTGGGCAACCCAAGCTTGATCCACGCACAAAG SRR13695423 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 02:27:16 Started mapping on | Feb 12 02:27:16 Finished on | Feb 12 02:31:17 Mapping speed, Million of reads per hour | 257.00 Number of input reads | 17204548 Average input read length | 293 UNIQUE READS: Uniquely mapped reads number | 15093955 Uniquely mapped reads % | 87.73% Average mapped length | 292.68 Number of splices: Total | 14140955 Number of splices: Annotated (sjdb) | 13883158 Number of splices: GT/AG | 13827810 Number of splices: GC/AG | 263125 Number of splices: AT/AC | 8555 Number of splices: Non-canonical | 41465 Mismatch rate per base, % | 0.36% Deletion rate per base | 0.03% Deletion average length | 2.84 Insertion rate per base | 0.02% Insertion average length | 2.24 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 398315 % of reads mapped to multiple loci | 2.32% Number of reads mapped to too many loci | 40310 % of reads mapped to too many loci | 0.23% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 9.60% % of reads unmapped: other | 0.12% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1712472 1712472 1712472 N_multimapping 398315 398315 398315 N_noFeature 333549 14722367 473039 N_ambiguous 326992 1329 94243 UnstrandedReadsAssigned:14433414 PositiveStrandReadsAssigned:370259 NegativeStrandReadsAssigned:14526673 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695423 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695423-trimmed-pair1.fastq SRR13695423-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 17,204,548 reads, 14,644,579 reads pseudoaligned [quant] estimated average fragment length: 224.332 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,348 rounds 52401 SRR13695423.ke.tsv 34699 SRR13695423.se.tsv 87100 total ==> SRR13695423.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1794.67 706 20.3279 Potri.005G024800.1.v4.1 1035 811.668 500 31.832 Potri.004G059700.1.v4.1 961 737.682 1 0.070049 Potri.007G009000.2.v4.1 1416 1192.67 0 0 Potri.003G141000.2.v4.1 2943 2719.67 648.042 12.3129 Potri.016G087400.1.v4.1 270 89.6887 774.756 446.374 Potri.015G069301.1.v4.1 564 344.002 0 0 Potri.010G195200.1.v4.1 1773 1549.67 211 7.03583 Potri.012G127500.1.v4.1 977 753.678 165 11.3128 ==> SRR13695423.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 158 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 485 Potri.001G212900.v4.1 89 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 2 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 6 SRR13695423 completed mapping pipeline successfully