Starting /dee2/code/volunteer_pipeline.sh SRR13695424 current disk space = 3049856851968 free memory = 1424537232 SRR13695424 SRAfilesize e8e24a476d1190ace34b86aeb41962df SRR13695424.sra SRR13695424.sra file validated SRR13695424 is paired end SRR13695424 is conventional basespace SRR13695424 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695424_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.451 37.0 37.0 37.0 37.0 37.0 2 36.24875 37.0 37.0 37.0 37.0 37.0 3 36.518 37.0 37.0 37.0 37.0 37.0 4 36.584 37.0 37.0 37.0 37.0 37.0 5 36.511 37.0 37.0 37.0 37.0 37.0 6 36.5335 37.0 37.0 37.0 37.0 37.0 7 36.532 37.0 37.0 37.0 37.0 37.0 8 36.579 37.0 37.0 37.0 37.0 37.0 9 36.5815 37.0 37.0 37.0 37.0 37.0 10-14 36.5446 37.0 37.0 37.0 37.0 37.0 15-19 36.507799999999996 37.0 37.0 37.0 37.0 37.0 20-24 36.5349 37.0 37.0 37.0 37.0 37.0 25-29 36.4905 37.0 37.0 37.0 37.0 37.0 30-34 36.4068 37.0 37.0 37.0 37.0 37.0 35-39 36.403499999999994 37.0 37.0 37.0 37.0 37.0 40-44 36.3885 37.0 37.0 37.0 37.0 37.0 45-49 36.3092 37.0 37.0 37.0 37.0 37.0 50-54 36.3953 37.0 37.0 37.0 37.0 37.0 55-59 36.3429 37.0 37.0 37.0 37.0 37.0 60-64 36.3874 37.0 37.0 37.0 37.0 37.0 65-69 36.2999 37.0 37.0 37.0 37.0 37.0 70-74 36.257799999999996 37.0 37.0 37.0 37.0 37.0 75-79 36.2638 37.0 37.0 37.0 37.0 37.0 80-84 36.2032 37.0 37.0 37.0 37.0 37.0 85-89 36.1894 37.0 37.0 37.0 37.0 37.0 90-94 36.1242 37.0 37.0 37.0 37.0 37.0 95-99 36.1471 37.0 37.0 37.0 37.0 37.0 100-104 36.094 37.0 37.0 37.0 37.0 37.0 105-109 36.074 37.0 37.0 37.0 37.0 37.0 110-114 35.991200000000006 37.0 37.0 37.0 37.0 37.0 115-119 35.986900000000006 37.0 37.0 37.0 37.0 37.0 120-124 35.9405 37.0 37.0 37.0 37.0 37.0 125-129 35.865300000000005 37.0 37.0 37.0 37.0 37.0 130-134 36.007999999999996 37.0 37.0 37.0 37.0 37.0 135-139 35.8262 37.0 37.0 37.0 37.0 37.0 140-144 35.847699999999996 37.0 37.0 37.0 37.0 37.0 145-149 35.636700000000005 37.0 37.0 37.0 37.0 37.0 150-151 35.589 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 1.0 25 4.0 26 6.0 27 12.0 28 14.0 29 19.0 30 25.0 31 45.0 32 48.0 33 70.0 34 127.0 35 345.0 36 2975.0 37 309.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.325 10.8 8.1 46.775 2 18.99974868057301 12.79215883387786 37.82357376225182 30.38451872329731 3 17.724999999999998 18.625 26.700000000000003 36.95 4 21.575 26.125 22.95 29.349999999999998 5 21.125 32.95 24.474999999999998 21.45 6 20.150000000000002 33.550000000000004 26.35 19.950000000000003 7 15.7 25.650000000000002 43.2 15.45 8 19.15 24.2 31.624999999999996 25.025 9 16.7 25.4 34.75 23.150000000000002 10-14 19.335 30.095 27.215 23.355 15-19 19.744999999999997 28.115000000000002 27.384999999999998 24.755 20-24 19.405 28.84 27.950000000000003 23.805 25-29 20.09 27.38 28.749999999999996 23.78 30-34 19.5 28.125 28.035 24.34 35-39 19.31 29.12 27.650000000000002 23.919999999999998 40-44 20.125 28.265 27.944999999999997 23.665 45-49 19.615 27.310000000000002 28.785 24.29 50-54 19.695 29.215000000000003 26.935 24.154999999999998 55-59 20.055 28.744999999999997 27.47 23.73 60-64 19.09 28.810000000000002 27.665 24.435000000000002 65-69 20.72 27.834999999999997 27.779999999999998 23.665 70-74 20.495 28.449999999999996 27.655 23.400000000000002 75-79 19.57 28.410000000000004 28.46 23.56 80-84 19.89 28.59 28.720000000000002 22.8 85-89 19.88 28.585 28.17 23.365 90-94 19.37 27.91 28.57 24.15 95-99 19.775000000000002 28.88 27.750000000000004 23.595 100-104 20.080000000000002 28.95 27.29 23.68 105-109 20.31 28.610000000000003 27.500000000000004 23.580000000000002 110-114 20.31 28.835 27.065 23.79 115-119 20.11 28.54 27.88 23.47 120-124 20.16 27.900000000000002 28.055000000000003 23.885 125-129 20.59 28.465 27.405 23.54 130-134 21.175 27.88 27.825 23.119999999999997 135-139 19.650000000000002 28.48 28.035 23.835 140-144 20.145 28.57 26.805 24.48 145-149 21.455 28.165000000000003 27.405 22.975 150-151 22.3125 28.7375 26.787499999999998 22.162499999999998 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.5 22 0.5 23 0.5 24 1.5 25 2.0 26 5.5 27 8.0 28 12.0 29 16.5 30 15.0 31 27.0 32 41.0 33 56.0 34 69.0 35 80.5 36 99.5 37 104.0 38 135.5 39 161.0 40 170.0 41 223.0 42 260.0 43 254.0 44 250.5 45 273.0 46 257.5 47 235.0 48 234.5 49 204.5 50 157.0 51 124.5 52 118.5 53 93.5 54 66.5 55 63.5 56 45.5 57 26.5 58 35.0 59 28.0 60 10.0 61 8.5 62 8.5 63 5.0 64 3.5 65 2.0 66 2.5 67 2.5 68 0.5 69 0.0 70 0.0 71 0.0 72 0.5 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.525 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.5 #Duplication Level Percentage of deduplicated Percentage of total 1 66.47286821705426 42.875 2 20.232558139534884 26.1 3 8.604651162790699 16.650000000000002 4 2.558139534883721 6.6000000000000005 5 1.2790697674418605 4.125 6 0.5038759689922481 1.95 7 0.23255813953488372 1.05 8 0.03875968992248062 0.2 9 0.07751937984496124 0.44999999999999996 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CAAAATACAAGGTAAGAACAGGCATGAAAGCACAAATAACAGAGCAAACT 9 0.22499999999999998 No Hit CCAAAACCTCTTGCTCCACGGCCACGGCCACGACCCCGACTGCCTGAGCC 9 0.22499999999999998 No Hit GTCCAGTGAGACCTGCATCACCATGAGGACCACCAATAACAAAACGGCCA 8 0.2 No Hit CTTACTATGTTCCAACTATCCTGCTTGTGTTTGCTTCGAGTTCATCTTTC 7 0.17500000000000002 No Hit ATAGTGTAAGACATACCAGAGCATCCACCTTGTTTAACCCCAATTCTTAA 7 0.17500000000000002 No Hit GCCATGTTATCTTTTCCGGGCACTGCTGGCAGTAGCCGACGGAGTATTGA 7 0.17500000000000002 No Hit CCTGAGTAGCATGACTGTGATTTGTGCCTCCTACTTTAACTGCCTCATCC 7 0.17500000000000002 No Hit GTTGGAGAGAAGTCGAGCCATGGCAGGGCAGCTCATCTGCTTGTTGTGTT 7 0.17500000000000002 No Hit ACAGCTTGTGTTTTGAAGGACATTCTTTGGGAATGTGTTCCATTTGACAT 7 0.17500000000000002 No Hit GCAGTAGCAAGTTGGGTTCAACACCACCGATTGCATATCTCTTTCTCTCT 6 0.15 No Hit GCCATGGATGACCTCAAGAGCTCTGTTCTTAGCAAAGGCCTCAGGATCAG 6 0.15 No Hit GCCTCAGCCACTAGCTTGGCACTTGAGAGAAGCTCAGACGTAGTGGGTTG 6 0.15 No Hit GGGGGGGGTTTCTCTTATTTTTTGTTTCTTCTTCTTCTTCTTTTCCCCTA 6 0.15 No Hit GCACACTCAAAATCAAAGTAACTACGAGGGTCCATATCAAAGACTTCAAA 6 0.15 No Hit CCATCCACCCCAAAGGGAAAGTACCCAGTAGGAAGTTCATATCCAGCCCA 6 0.15 No Hit GGCATACTTTCCAACACCAGGAAGAAAGGTGACATGGGTCCAGTCCTCTT 6 0.15 No Hit ATTGACCTGAAATTTTGGCCCACGCCTTGGCGCCCTGGGTTCACCTTCTC 6 0.15 No Hit GGGTAAAATGTCATTGACAGTCACTTTGGTAGACACGTTAGAATAGGTAT 6 0.15 No Hit CACCAACTGAGCAACTTCCAATCCCAGTTGCTCCAATTCCCGACAAGGCT 6 0.15 No Hit GCTTGATAATATACAAACAAGATAAAATATATTAGTGCAGCTCTTTGGGG 6 0.15 No Hit GGACCGTTCATCAGCGTTGTAGATAGGGCTGTAACCATCCACATTAGCAC 6 0.15 No Hit GCTGCATCTTTCAATGTGCTGGCTTTGGTTGGCTGCCGGTTGAAGTAAAC 6 0.15 No Hit CTGGCATGTCAGGTCCAGCAAGCCCTTGATGTTCAGATAGTTGGCCGCCA 5 0.125 No Hit GGGGCTTTCGCATACTTGGTGGACATGATCTTTACAAAGGCCTGCCTCAG 5 0.125 No Hit CGTTCTTGACACCACCGCTGTCATTGACAACAATTACATCAAGGGGAGAG 5 0.125 No Hit CCAGGCACAATCTCTCTTGTAAGTCTAACAATTGCATCTTCAGCAGCGTT 5 0.125 No Hit GTTGTGGACAGGATCGGCCAGGTGATCCAGGAGGTTTTGGTATGGTCCGA 5 0.125 No Hit AGCACTTCTGCACTTCCCTTCCGGCACCTCTTTGAAGGATGATGAACCGC 5 0.125 No Hit AGCAACTTCTTTATGGCATATCGAACAGTGCTATGAGGTTTGCTTAATCC 5 0.125 No Hit ATCTATTTATCTTTACAGTGAGAGGAGTGGTTCCAAATCCAGGATATGCA 5 0.125 No Hit GGTAAAGCATTTTGCCAAGGTCTAAGTACAATTTAAACAAACCACCCCTA 5 0.125 No Hit GGATCTTTAGGCATGAGCATTTCTTTCCCAACTGGTCCTGTAATACTAAC 5 0.125 No Hit GCCCCATCTACCTCTGCTCCTTATTACAAGACCAGAATCCCACCTTATCC 5 0.125 No Hit TCCACTTGCAAGTGTTCTAAGAGAAAAGAAGGCATAACAGTCTTAAAACA 5 0.125 No Hit GCGTCTTGTTAGCGTCCGTAGGCAACCGTAGCCGCCGCTTCAATCCAAGG 5 0.125 No Hit CTCATGGCAGCCTCTGTCTCCAAAATCAGGCTATTTTTCTCTTTGTTCAG 5 0.125 No Hit CTCATAAGATTTGAAAATCAGGTTTCGGAATCCTGCTGTATTCAAGCTGT 5 0.125 No Hit AGCACGGTTAGCTAGCCTCCTTGCACGTTCCATGAGTGCAGCACACAAAC 5 0.125 No Hit GTTCCATTTTAGGTTTGCCAAACTGAAAGTCGATCCACACCTTCCCTTTG 5 0.125 No Hit GGCAAGTCCATCAAGACCTTGGCACCACGACTCGTCATTGGAACCAGCTA 5 0.125 No Hit GGCCATAAAAAAAATATTCGCGTACCTGGTCTACCCATGGAATTTTGGAA 5 0.125 No Hit TCCTCTTTAACAACATTGTTTACAGCTTTCACTCAATCATCATCAATCAT 5 0.125 No Hit CCCCAAACTCCAAAGCCAATCGCAATAGATTTAGTAGGAAAAAAAAATGT 5 0.125 No Hit GGTATTGCAAGCTATCATTGAAAATTACTTTCCAGAGAGGCATTCCACCA 5 0.125 No Hit CCTGGAGTTCCTGGCATAGTCATGGACCTCTGAAACTTATTAACAGGACT 5 0.125 No Hit GTTTTGCTTTGCTGTGCCTCTAGATATGCTGCGGCAGTCTCAGAATGTTT 5 0.125 No Hit CTCCGTTCACAACAACGCACGAGCACCCATCGTAATAAAGGCGGCCACCA 5 0.125 No Hit GGTTTAATGTATTTGCACCATCTACAAACCATGTTCCATTTCCTCCTTCA 5 0.125 No Hit GCCTTGTTTAAGAAGTTTGCGTTCTACTCGGCCAGTTTTAGGGCTCAATT 5 0.125 No Hit TGGCTATCAATCCACACTAACCCAATACCACCAATTCTTTTTCAAAGATT 5 0.125 No Hit AAGCTGTGTACCCACTCTTAAACTGCCGATACTCCTTACCCTTTTCACCA 5 0.125 No Hit GGCCAAGTAACCTATTCTGAAAATAGATAATTTCTCCTTCCACTTCGTCC 5 0.125 No Hit CACCTGATGACCCTCTTTGACAAGAAGTCTAGACAAGAACACACCAATGA 5 0.125 No Hit CCCTGCACCTCAACTAAGCTTATTAGTTTCCATATCTAATTGTCCCACAT 5 0.125 No Hit CATTCTTGTACACCAGCTACCTTGAACAAATTTCTCCTACCAATATATCC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.037500000000000006 0.0 0.0 0.0 0.0 88-89 0.05 0.0 0.0 0.0 0.0 90-91 0.05 0.0 0.0 0.0 0.0 92-93 0.125 0.0 0.0 0.0 0.0 94-95 0.175 0.0 0.0 0.0 0.0 96-97 0.325 0.0 0.0 0.0 0.0 98-99 0.35 0.0 0.0 0.0 0.0 100-101 0.5125 0.0 0.0 0.0 0.0 102-103 0.825 0.0 0.0 0.0 0.0 104-105 0.9875 0.0 0.0 0.0 0.0 106-107 1.2 0.0 0.0 0.0 0.0 108-109 1.3 0.0 0.0 0.0 0.0 110-111 1.6625 0.0 0.0 0.0 0.0 112-113 1.8375 0.0 0.0 0.0 0.0 114-115 2.075 0.0 0.0 0.0 0.0 116-117 2.2625 0.0 0.0 0.0 0.0 118-119 2.6875 0.0 0.0 0.0 0.0 120-121 3.1 0.0 0.0 0.0 0.0 122-123 3.55 0.0 0.0 0.0 0.0 124-125 3.9000000000000004 0.0 0.0 0.0 0.0 126-127 4.25 0.0 0.0 0.0 0.0 128-129 4.9125 0.0 0.0 0.0 0.0 130-131 5.225 0.0 0.0 0.0 0.0 132-133 5.7 0.0 0.0 0.0 0.0 134-135 6.075 0.0 0.0 0.0 0.0 136-137 6.7 0.0 0.0 0.0 0.0 138-139 7.2875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGCATAC 10 0.006830828 145.0 1 GGTCAGT 10 0.006830828 145.0 1 AGTATAA 10 0.006830828 145.0 5 ATACTTT 10 0.006830828 145.0 4 CATACTT 10 0.006830828 145.0 3 GCATACT 10 0.006830828 145.0 2 >>END_MODULE SRR13695424 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695424_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.0955 37.0 37.0 37.0 37.0 37.0 2 36.163 37.0 37.0 37.0 37.0 37.0 3 36.3105 37.0 37.0 37.0 37.0 37.0 4 36.2305 37.0 37.0 37.0 37.0 37.0 5 36.4095 37.0 37.0 37.0 37.0 37.0 6 36.3015 37.0 37.0 37.0 37.0 37.0 7 36.317 37.0 37.0 37.0 37.0 37.0 8 36.429 37.0 37.0 37.0 37.0 37.0 9 36.3435 37.0 37.0 37.0 37.0 37.0 10-14 36.306599999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.2919 37.0 37.0 37.0 37.0 37.0 20-24 36.21785 37.0 37.0 37.0 37.0 37.0 25-29 36.205349999999996 37.0 37.0 37.0 37.0 37.0 30-34 36.164100000000005 37.0 37.0 37.0 37.0 37.0 35-39 36.13095 37.0 37.0 37.0 37.0 37.0 40-44 36.11025 37.0 37.0 37.0 37.0 37.0 45-49 36.181400000000004 37.0 37.0 37.0 37.0 37.0 50-54 36.02265 37.0 37.0 37.0 37.0 37.0 55-59 36.078450000000004 37.0 37.0 37.0 37.0 37.0 60-64 36.02804999999999 37.0 37.0 37.0 37.0 37.0 65-69 36.02345 37.0 37.0 37.0 37.0 37.0 70-74 35.91985 37.0 37.0 37.0 37.0 37.0 75-79 35.899449999999995 37.0 37.0 37.0 37.0 37.0 80-84 35.9701 37.0 37.0 37.0 37.0 37.0 85-89 35.881150000000005 37.0 37.0 37.0 37.0 37.0 90-94 35.863249999999994 37.0 37.0 37.0 37.0 37.0 95-99 35.86719999999999 37.0 37.0 37.0 37.0 37.0 100-104 35.80365 37.0 37.0 37.0 37.0 37.0 105-109 35.7815 37.0 37.0 37.0 37.0 37.0 110-114 35.76065 37.0 37.0 37.0 37.0 37.0 115-119 35.709050000000005 37.0 37.0 37.0 37.0 37.0 120-124 35.64874999999999 37.0 37.0 37.0 37.0 37.0 125-129 35.697900000000004 37.0 37.0 37.0 37.0 37.0 130-134 35.57995 37.0 37.0 37.0 34.6 37.0 135-139 35.6374 37.0 37.0 37.0 37.0 37.0 140-144 35.504450000000006 37.0 37.0 37.0 34.6 37.0 145-149 35.39315 37.0 37.0 37.0 34.6 37.0 150-151 35.000125 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 4.0 14 1.0 15 0.0 16 1.0 17 1.0 18 0.0 19 1.0 20 0.0 21 0.0 22 3.0 23 3.0 24 1.0 25 5.0 26 7.0 27 6.0 28 18.0 29 15.0 30 18.0 31 39.0 32 65.0 33 116.0 34 219.0 35 584.0 36 2664.0 37 227.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.23355097940733 19.83927674535409 13.033651431441488 31.893520843797084 2 24.875 24.474999999999998 34.675 15.975 3 21.5 27.825 30.275000000000002 20.4 4 22.95 35.275 22.525000000000002 19.25 5 24.75 36.175000000000004 22.725 16.35 6 19.6 39.375 23.474999999999998 17.549999999999997 7 20.0 21.825 39.300000000000004 18.875 8 19.975 26.55 30.725 22.75 9 24.025 24.125 29.049999999999997 22.8 10-14 22.650000000000002 29.45 27.625 20.275000000000002 15-19 22.79 28.754999999999995 27.865000000000002 20.59 20-24 22.889878420973634 28.338419972982436 27.93815980387252 20.833541802171414 25-29 22.2377972465582 28.16520650813517 28.821026282853566 20.77596996245307 30-34 22.39515564007607 27.985186668001198 28.77089380442398 20.84876388749875 35-39 22.404562965927855 28.888777705508577 27.27272727272727 21.433932055836294 40-44 23.217056203393224 28.612181572493867 28.131725138881936 20.03903708523097 45-49 23.1935548438751 28.047437950360287 28.47277822257806 20.28622898318655 50-54 23.27512883374193 28.408465502576675 28.023215089808375 20.29319057387302 55-59 23.123904881101375 27.11389236545682 29.04630788485607 20.715894868585732 60-64 22.83484264772102 28.443488267373795 27.703006954520436 21.01866213038475 65-69 22.332816485770017 27.914770169559343 29.070174561096383 20.68223878357425 70-74 22.993742177722154 28.010012515644554 28.355444305381727 20.640801001251567 75-79 22.751614033331666 28.256844001801714 28.381962864721483 20.609579100145137 80-84 22.50625312656328 27.498749374687343 28.67433716858429 21.320660330165083 85-89 22.445812684587274 28.342593983080544 28.22746158081794 20.98413175151424 90-94 23.79546705358483 28.433481763146045 27.28273377695502 20.488317406314106 95-99 23.859315589353614 27.04622773664199 27.996798078847306 21.097658595157096 100-104 23.219023779724658 28.28535669586984 27.554443053817273 20.941176470588236 105-109 23.28293952743292 27.913496195434522 28.188826591910292 20.614737685222266 110-114 23.50027517886626 27.89313053484765 28.223345174363335 20.383249111922748 115-119 23.10657255844221 29.108474745957853 27.49662111428142 20.288331581318516 120-124 23.083854818523154 27.53441802252816 28.315394242803503 21.066332916145182 125-129 23.73610972069276 28.14095505055561 28.03083391730904 20.092101311442587 130-134 24.357883142242027 28.253141741350824 27.587242777749964 19.80173233865719 135-139 24.822268949634523 29.04776209071793 26.219084810253328 19.91088414939421 140-144 25.481563015960372 27.137639465652676 27.427828088257368 19.95296943012958 145-149 25.436795994993744 28.69086357947434 27.37421777221527 18.498122653316646 150-151 26.436349981224183 26.974590061334336 27.63800225309801 18.951057704343473 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 2.0 10 1.5 11 0.0 12 0.0 13 1.5 14 1.5 15 0.5 16 0.5 17 0.0 18 0.0 19 0.5 20 1.0 21 1.0 22 1.0 23 2.0 24 4.0 25 2.5 26 6.0 27 10.0 28 7.5 29 12.0 30 25.5 31 34.0 32 34.5 33 41.5 34 69.5 35 87.5 36 80.5 37 111.5 38 136.5 39 166.5 40 201.5 41 216.0 42 253.0 43 287.0 44 298.0 45 289.0 46 262.0 47 233.0 48 211.5 49 179.5 50 155.5 51 127.0 52 98.5 53 75.5 54 71.0 55 61.5 56 26.5 57 20.5 58 31.5 59 22.0 60 10.5 61 8.5 62 8.0 63 5.5 64 0.5 65 0.0 66 0.0 67 0.0 68 0.5 69 0.5 70 0.0 71 0.5 72 0.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.5 78 0.5 79 0.0 80 0.0 81 0.0 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.44999999999999996 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.065 25-29 0.125 30-34 0.09 35-39 0.065 40-44 0.095 45-49 0.08 50-54 0.065 55-59 0.125 60-64 0.065 65-69 0.034999999999999996 70-74 0.125 75-79 0.095 80-84 0.05 85-89 0.11499999999999999 90-94 0.065 95-99 0.06 100-104 0.125 105-109 0.12 110-114 0.065 115-119 0.11499999999999999 120-124 0.125 125-129 0.11 130-134 0.135 135-139 0.13 140-144 0.065 145-149 0.125 150-151 0.13749999999999998 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.125 #Duplication Level Percentage of deduplicated Percentage of total 1 67.447216890595 43.925 2 19.577735124760075 25.5 3 8.253358925143955 16.125 4 2.6871401151631478 7.000000000000001 5 1.2667946257197698 4.125 6 0.42226487523992323 1.6500000000000001 7 0.2687140115163148 1.225 8 0.0 0.0 9 0.07677543186180423 0.44999999999999996 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CATAGTATTAGCTGGAGGATGGGTTTTCACTGTAGCTTTAGCTGCCGTTG 9 0.22499999999999998 No Hit CAGCAGCAGATAGGAAAAAGGAGAACCCTGCTAATGAACCTGAAGAGAAA 9 0.22499999999999998 No Hit CTTCTCATCAAACCTTTGATTAATCGATCAATCAATCGCCGTAGTGAGTC 7 0.17500000000000002 No Hit TCAAATTTTACCATTATTCATCGAGACATAAAGAGTAGCAACGTTCTATT 7 0.17500000000000002 No Hit GAGAAGCAGAAGCTAAGTCAGCAATGGCAGCCTCAGTAATGGCTTCATTG 7 0.17500000000000002 No Hit CTCTCTCTAAGCCATAGCAACCCCTCTCTCTACCAGCACCCAATGACTAA 7 0.17500000000000002 No Hit CCAGATTCAGTTTGCCATGGCCTACTACGTGATCAACTACTCCAAGCTCC 7 0.17500000000000002 No Hit TTCCTAGAATTTGAAACATGGAAGCTTGGTGATTTTGATAAAGGTTTATA 7 0.17500000000000002 No Hit AACTGGAGGATTCTTGACTTCTTCTCCCACCATCCTGAAAGTTTGCACAT 7 0.17500000000000002 No Hit CAACCTCAGGGCACCACAAGTTCTTGTACAGAAACCAGCTTCTTGGACCA 6 0.15 No Hit AAGCACTGGTGGGCTGTTGAGGAAAGCCCAGGTGGAGGAGTTTTATGTGA 6 0.15 No Hit CTTCGAGAGAGATCACTAAGTTCTAACCCAGGGAAAAACAGTAAGGATTG 6 0.15 No Hit CATCAGCACCCCAGCTTCTTTCATTTACCAGACAAACAGACAAAGCTCTC 6 0.15 No Hit TTCATATATAAGCCGCCGCCTGAATCCGCCACGAGAGCCGCCGAGCTAGT 6 0.15 No Hit AGAAAATTATCTGCAAGCCTCTCTTGATTCTCGCTGCTGTTTCCTAGCTA 6 0.15 No Hit GTGGGAATAAAGGAGAGTACTTTAGCACAAGCCATAGTAACCTCCATAAA 6 0.15 No Hit CTTACAACATTGATATATATTCCACACTCCTCAAGGTTACAACCACAAGA 6 0.15 No Hit ATAAAATAAGTGAGGTAGAGGGTCTCCACCGTTTGTTGAAATTAACGGTA 6 0.15 No Hit GACCGATACGCCTTATGGAACTGGTGGTGGCATGAACCTGAGGAACGGGT 6 0.15 No Hit GGAGAGTTCTCGTCATTTGTATGCTATTAAACTGCACATCGGGTGGCCAG 6 0.15 No Hit CTCCTCAACACTAAAACCAAAACAACCCCTTTTCCAACCAAATTTCCCAT 5 0.125 No Hit GTTTTTACGAGTCAAGATTTGATTTTTATGCTTTGAGTTCATCAGGTTCA 5 0.125 No Hit GGAAAGTTGCCCCCGATCCAGTAGCAATTGATGCTCCTGGGGGGTTTCTT 5 0.125 No Hit GGAAAAAGACTTGCACAGGTTGTGAGTGATCCGAGCCTGACGAAATCTGG 5 0.125 No Hit GAGCTTTTCACCCCTATCCCTCCTCATGCTGAGCTTGCGCTCAATGGGGT 5 0.125 No Hit CTGAAGGTTGGGGTGGGTGGGATTTGGAGCCTACATTGTCAAAGCTGAAT 5 0.125 No Hit GCCAGAAGCGTATTCTAATTCTTATGTCCATCCATGTATACATGAGAAGC 5 0.125 No Hit GGAATTGGCCAAGGAAATTGATTACCTTCTTCGCTCGAAGTGGGTTCCTT 5 0.125 No Hit GCAGTATTTCCTGGGATTCGAGAAGTATTTGGGTGGGTCCGGAGAACCAG 5 0.125 No Hit GTCAACTCCTGTTAGGGCAGAAACTGTGGCCAAATTAAAGGTGGCTATCA 5 0.125 No Hit AAAACGTGTAAAGAAAAGGGCCTCAAGGTGTATGAAAATGTTCGAATGTT 5 0.125 No Hit TCTCATTGTATATAATACTACTAGTTATAGAGAGTGAGTGACAACTAGTA 5 0.125 No Hit AGGAAGGAGGCGAGCACTCTACAGCTGTTGAACCCAGGGCAGAGAAATAG 5 0.125 No Hit GTTTCAAGAAACTTGGAAAGGCAAAGAGAGGATAGTAGTAGTAGAGACTA 5 0.125 No Hit CTTTGATGACATGAACACAGAGAAGTTGCGAATGGCAGCAGGAGAGAACA 5 0.125 No Hit TGCCTGTTAACTTCGAGAAAGTACTGATACCTAAGTATCCTGACCATTTT 5 0.125 No Hit AGAAAATTGGAGGAGGAGTGCATGAAGGGATTGGTCTTGAATGCATGCTC 5 0.125 No Hit CATCACTTCACTTTATTTCCTCTCTCTCTCAACAACCCCATTTCCTTTTT 5 0.125 No Hit GCTAGAGAACACTGAGGCTAACCGCCAGGCATACCGAACCCTTCTTGTGA 5 0.125 No Hit CACGAGGCTGCGAGGCGTGCTGCATCGTTGCCCATTTTTCTTTGCCATGG 5 0.125 No Hit ATCAACCACTTGATCCATTAGCTGAGAAACTTCGCCAGCAAAGGCTGGTG 5 0.125 No Hit GAACCCTTACACTGGAAGATGTCTTTTGAACACTAAGATCACTGGTGATG 5 0.125 No Hit GTTTTTTTCTTTGTGGGTGGTGATTTCAGATTTTTGAGCTGCTGATGGAT 5 0.125 No Hit AAGTCATACAACTAATAAAGTCAAATATGTGAGTAGTTACTCAGGTGGAG 5 0.125 No Hit CGAGAATGATCGCCCTTCTATCCGCAAGGAAGAAGGGTCAAGTTCAGACT 5 0.125 No Hit GTGTCAGGGCAGTCTTGGTTGACAAGGATCAGAAACCAAAGTGGAATCCG 5 0.125 No Hit AATGAAATTACTACTTGCTCAAGTAAAAATGCTTGCTTTGAGCGAGGTCT 5 0.125 No Hit GTATCTTCCAACGACCTCGACGATCCTTTAATCATACAAGTCGTATCAGA 5 0.125 No Hit CTTCAATGACAATGGTGCAATGGTCCCTGTTCGTGTCCACACTGTTCTCA 5 0.125 No Hit CTTTGATCAAGATTCAAGAAGAAGAGGAGGTCATCAGAAATTTGAAGCTT 5 0.125 No Hit ACTGTGTCTGGTAGGCCACTGGATTTGGCTGCTGCCATGTACAGGGTCCC 5 0.125 No Hit GTGAAAGATTGCACGTGCTGTGTATGATGTTGAGGAGGCTACAGCCTTTG 5 0.125 No Hit TGAGACTCCAAAACCCGAGGATCGAGGAACTAACAGCGGCGATGATGAGC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.037500000000000006 0.0 0.0 0.0 0.0 88-89 0.05 0.0 0.0 0.0 0.0 90-91 0.05 0.0 0.0 0.0 0.0 92-93 0.125 0.0 0.0 0.0 0.0 94-95 0.175 0.0 0.0 0.0 0.0 96-97 0.325 0.0 0.0 0.0 0.0 98-99 0.35 0.0 0.0 0.0 0.0 100-101 0.5125 0.0 0.0 0.0 0.0 102-103 0.825 0.0 0.0 0.0 0.0 104-105 0.9875 0.0 0.0 0.0 0.0 106-107 1.2 0.0 0.0 0.0 0.0 108-109 1.3 0.0 0.0 0.0 0.0 110-111 1.6625 0.0 0.0 0.0 0.0 112-113 1.8375 0.0 0.0 0.0 0.0 114-115 2.0875000000000004 0.0 0.0 0.0 0.0 116-117 2.2875 0.0 0.0 0.0 0.0 118-119 2.7125 0.0 0.0 0.0 0.0 120-121 3.125 0.0 0.0 0.0 0.0 122-123 3.575 0.0 0.0 0.0 0.0 124-125 3.925 0.0 0.0 0.0 0.0 126-127 4.275 0.0 0.0 0.0 0.0 128-129 4.9375 0.0 0.0 0.0 0.0 130-131 5.25 0.0 0.0 0.0 0.0 132-133 5.725 0.0 0.0 0.0 0.0 134-135 6.1 0.0 0.0 0.0 0.0 136-137 6.7 0.0 0.0 0.0 0.0 138-139 7.2875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTTAGG 10 0.0067867227 145.29115 145 GTTCTCG 10 0.0070502195 143.475 6 GAGTTCT 10 0.0070502195 143.475 4 AGAGTTC 10 0.0070502195 143.475 3 TTCTCGT 10 0.0070502195 143.475 7 GAGAGTT 10 0.0070502195 143.475 2 TGGAATT 10 0.0070502195 143.475 2 CTCGTCA 10 0.0070502195 143.475 9 >>END_MODULE Read 1283019 spots for SRR13695424.sra Written 1283019 spots for SRR13695424.sra Read 1283019 spots for SRR13695424.sra Written 1283019 spots for SRR13695424.sra Read 1283019 spots for SRR13695424.sra Written 1283019 spots for SRR13695424.sra Read 1283019 spots for SRR13695424.sra Written 1283019 spots for SRR13695424.sra Read 1283019 spots for SRR13695424.sra Written 1283019 spots for SRR13695424.sra Read 1283019 spots for SRR13695424.sra Written 1283019 spots for SRR13695424.sra Read 1283019 spots for SRR13695424.sra Written 1283019 spots for SRR13695424.sra Read 1283019 spots for SRR13695424.sra Written 1283019 spots for SRR13695424.sra Read 1283019 spots for SRR13695424.sra Written 1283019 spots for SRR13695424.sra Read 1283019 spots for SRR13695424.sra Written 1283019 spots for SRR13695424.sra Read 1283019 spots for SRR13695424.sra Written 1283019 spots for SRR13695424.sra Read 1283023 spots for SRR13695424.sra Written 1283023 spots for SRR13695424.sra Read 1283019 spots for SRR13695424.sra Written 1283019 spots for SRR13695424.sra Read 1283019 spots for SRR13695424.sra Written 1283019 spots for SRR13695424.sra Read 1283019 spots for SRR13695424.sra Written 1283019 spots for SRR13695424.sra Read 1283019 spots for SRR13695424.sra Written 1283019 spots for SRR13695424.sra Read 1283019 spots for SRR13695424.sra Written 1283019 spots for SRR13695424.sra Read 1283019 spots for SRR13695424.sra Written 1283019 spots for SRR13695424.sra Read 1283019 spots for SRR13695424.sra Written 1283019 spots for SRR13695424.sra Read 1283019 spots for SRR13695424.sra Written 1283019 spots for SRR13695424.sra SRR ids: ['SRR13695424.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_6j5ou71g SRR13695424.sra spots: 25660384 blocks: [[1, 1283019], [1283020, 2566038], [2566039, 3849057], [3849058, 5132076], [5132077, 6415095], [6415096, 7698114], [7698115, 8981133], [8981134, 10264152], [10264153, 11547171], [11547172, 12830190], [12830191, 14113209], [14113210, 15396228], [15396229, 16679247], [16679248, 17962266], [17962267, 19245285], [19245286, 20528304], [20528305, 21811323], [21811324, 23094342], [23094343, 24377361], [24377362, 25660384]] SRR13695424 file size 8698820 SRR13695424 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695424 SRR13695424_1.fastq SRR13695424_2.fastq Input file: SRR13695424_1.fastq Paired file: SRR13695424_2.fastq trimmed: SRR13695424-trimmed-pair1.fastq, SRR13695424-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 02:09:59 2025 >> started Wed Feb 12 02:10:25 2025 >> done (26.428s) 25660384 read pairs processed; of these: 214 ( 0.00%) short read pairs filtered out after trimming by size control 1091 ( 0.00%) empty read pairs filtered out after trimming by size control 25659079 (99.99%) read pairs available; of these: 2438259 ( 9.50%) trimmed read pairs available after processing 23220820 (90.50%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 1 0.00% 20 2 0.00% 21 3 0.00% 22 3 0.00% 23 1 0.00% 24 2 0.00% 25 4 0.00% 26 4 0.00% 27 8 0.00% 28 2 0.00% 29 5 0.00% 30 4 0.00% 31 3 0.00% 32 11 0.00% 33 6 0.00% 34 8 0.00% 35 11 0.00% 36 8 0.00% 37 7 0.00% 38 7 0.00% 39 12 0.00% 40 6 0.00% 41 21 0.00% 42 26 0.00% 43 15 0.00% 44 26 0.00% 45 38 0.00% 46 30 0.00% 47 26 0.00% 48 38 0.00% 49 49 0.00% 50 65 0.00% 51 79 0.00% 52 79 0.00% 53 93 0.00% 54 95 0.00% 55 97 0.00% 56 101 0.00% 57 115 0.00% 58 149 0.00% 59 178 0.00% 60 213 0.00% 61 248 0.00% 62 228 0.00% 63 305 0.00% 64 361 0.00% 65 413 0.00% 66 381 0.00% 67 518 0.00% 68 555 0.00% 69 648 0.00% 70 780 0.00% 71 885 0.00% 72 1070 0.00% 73 1187 0.00% 74 1383 0.01% 75 1462 0.01% 76 1651 0.01% 77 1900 0.01% 78 2152 0.01% 79 2372 0.01% 80 2753 0.01% 81 3235 0.01% 82 3520 0.01% 83 3987 0.02% 84 4669 0.02% 85 5174 0.02% 86 5551 0.02% 87 5718 0.02% 88 6498 0.03% 89 6840 0.03% 90 7437 0.03% 91 8468 0.03% 92 8725 0.03% 93 9680 0.04% 94 10533 0.04% 95 11414 0.04% 96 12400 0.05% 97 13107 0.05% 98 13857 0.05% 99 14580 0.06% 100 15666 0.06% 101 16254 0.06% 102 17406 0.07% 103 18490 0.07% 104 19341 0.08% 105 20425 0.08% 106 21596 0.08% 107 22597 0.09% 108 24007 0.09% 109 25021 0.10% 110 25695 0.10% 111 26601 0.10% 112 27833 0.11% 113 29035 0.11% 114 29544 0.12% 115 31678 0.12% 116 33083 0.13% 117 33633 0.13% 118 35754 0.14% 119 36281 0.14% 120 37425 0.15% 121 38553 0.15% 122 40039 0.16% 123 40872 0.16% 124 42794 0.17% 125 43577 0.17% 126 45809 0.18% 127 47290 0.18% 128 47702 0.19% 129 48566 0.19% 130 50872 0.20% 131 50707 0.20% 132 52820 0.21% 133 53668 0.21% 134 54766 0.21% 135 55933 0.22% 136 57502 0.22% 137 58745 0.23% 138 60272 0.23% 139 62479 0.24% 140 62681 0.24% 141 63974 0.25% 142 64792 0.25% 143 65545 0.26% 144 67871 0.26% 145 69561 0.27% 146 70402 0.27% 147 71484 0.28% 148 73859 0.29% 149 74156 0.29% 150 75306 0.29% 151 23220820 90.50% 25659079 reads passed initial QC criterion=sequence-density sequence-density=0.24 sequence-density-rank=1 fanout-score=3.74 fanout-score-rank=17 prefix-density=0.31 prefix-fanout=2.9 sequence=CTGATGCACTGCACTTGACG criterion=fanout-score sequence-density=0.01 sequence-density-rank=37 fanout-score=34.14 fanout-score-rank=1 prefix-density=0.09 prefix-fanout=2.3 sequence=ACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGAGTATGAATGTGTTCTCTG criterion=sequence-density sequence-density=0.41 sequence-density-rank=1 fanout-score=2.01 fanout-score-rank=35 prefix-density=0.42 prefix-fanout=2.0 sequence=TGTAAGAGATGGCTTCCTC criterion=fanout-score sequence-density=0.08 sequence-density-rank=30 fanout-score=62.49 fanout-score-rank=1 prefix-density=0.50 prefix-fanout=9.6 sequence=AGAAAAGAAAACAGATTATCAAGCTTACTAGAATTATGGAAGGAATGAGTGTGGAGAACATGCACAAGATAGTGGTGGCAGTGGATGAGAGTGAGGAGAGCATGCATGCTCTTTCATGGTGTCTCAGCAACCTTATTTCTCACAACTCCACCGCCACGTTAGTCCTCCTCTAT SRR13695424 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 02:11:05 Started mapping on | Feb 12 02:11:05 Finished on | Feb 12 02:14:24 Mapping speed, Million of reads per hour | 464.18 Number of input reads | 25659079 Average input read length | 297 UNIQUE READS: Uniquely mapped reads number | 23958609 Uniquely mapped reads % | 93.37% Average mapped length | 296.23 Number of splices: Total | 23139766 Number of splices: Annotated (sjdb) | 22629542 Number of splices: GT/AG | 22682529 Number of splices: GC/AG | 365960 Number of splices: AT/AC | 14000 Number of splices: Non-canonical | 77277 Mismatch rate per base, % | 0.37% Deletion rate per base | 0.03% Deletion average length | 2.93 Insertion rate per base | 0.02% Insertion average length | 2.44 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 566617 % of reads mapped to multiple loci | 2.21% Number of reads mapped to too many loci | 145320 % of reads mapped to too many loci | 0.57% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.73% % of reads unmapped: other | 0.13% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1134165 1134165 1134165 N_multimapping 566617 566617 566617 N_noFeature 1012673 23432425 1365590 N_ambiguous 319301 2592 144126 UnstrandedReadsAssigned:22626635 PositiveStrandReadsAssigned:523592 NegativeStrandReadsAssigned:22448893 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695424 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695424-trimmed-pair1.fastq SRR13695424-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 25,659,079 reads, 22,528,765 reads pseudoaligned [quant] estimated average fragment length: 254.188 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,070 rounds 52401 SRR13695424.ke.tsv 34699 SRR13695424.se.tsv 87100 total ==> SRR13695424.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1764.81 829 21.2306 Potri.005G024800.1.v4.1 1035 781.812 231 13.3541 Potri.004G059700.1.v4.1 961 707.913 21 1.34074 Potri.007G009000.2.v4.1 1416 1162.81 0 0 Potri.003G141000.2.v4.1 2943 2689.81 1087 18.2648 Potri.016G087400.1.v4.1 270 81.3157 923 513.019 Potri.015G069301.1.v4.1 564 320.505 0 0 Potri.010G195200.1.v4.1 1773 1519.81 126 3.74703 Potri.012G127500.1.v4.1 977 723.863 264 16.4837 ==> SRR13695424.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 546 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 318 Potri.001G212900.v4.1 10 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 7 Potri.001G416900.v4.1 3 Potri.001G452600.v4.1 6 SRR13695424 completed mapping pipeline successfully