Starting /dee2/code/volunteer_pipeline.sh SRR13695425 current disk space = 3049201455104 free memory = 1579620692 SRR13695425 SRAfilesize e294988dd3c171ea9a23d15109b1095b SRR13695425.sra SRR13695425.sra file validated SRR13695425 is paired end SRR13695425 is conventional basespace SRR13695425 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695425_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5555 37.0 37.0 37.0 37.0 37.0 2 36.366 37.0 37.0 37.0 37.0 37.0 3 36.6235 37.0 37.0 37.0 37.0 37.0 4 36.538 37.0 37.0 37.0 37.0 37.0 5 36.615 37.0 37.0 37.0 37.0 37.0 6 36.5395 37.0 37.0 37.0 37.0 37.0 7 36.522 37.0 37.0 37.0 37.0 37.0 8 36.552 37.0 37.0 37.0 37.0 37.0 9 36.4325 37.0 37.0 37.0 37.0 37.0 10-14 36.55 37.0 37.0 37.0 37.0 37.0 15-19 36.5295 37.0 37.0 37.0 37.0 37.0 20-24 36.517500000000005 37.0 37.0 37.0 37.0 37.0 25-29 36.49380000000001 37.0 37.0 37.0 37.0 37.0 30-34 36.470099999999995 37.0 37.0 37.0 37.0 37.0 35-39 36.4563 37.0 37.0 37.0 37.0 37.0 40-44 36.436 37.0 37.0 37.0 37.0 37.0 45-49 36.3981 37.0 37.0 37.0 37.0 37.0 50-54 36.4002 37.0 37.0 37.0 37.0 37.0 55-59 36.4056 37.0 37.0 37.0 37.0 37.0 60-64 36.3637 37.0 37.0 37.0 37.0 37.0 65-69 36.352199999999996 37.0 37.0 37.0 37.0 37.0 70-74 36.3564 37.0 37.0 37.0 37.0 37.0 75-79 36.29299999999999 37.0 37.0 37.0 37.0 37.0 80-84 36.254 37.0 37.0 37.0 37.0 37.0 85-89 36.2448 37.0 37.0 37.0 37.0 37.0 90-94 36.1032 37.0 37.0 37.0 37.0 37.0 95-99 36.129900000000006 37.0 37.0 37.0 37.0 37.0 100-104 36.1555 37.0 37.0 37.0 37.0 37.0 105-109 36.15990000000001 37.0 37.0 37.0 37.0 37.0 110-114 36.0798 37.0 37.0 37.0 37.0 37.0 115-119 36.1004 37.0 37.0 37.0 37.0 37.0 120-124 35.960499999999996 37.0 37.0 37.0 37.0 37.0 125-129 35.997499999999995 37.0 37.0 37.0 37.0 37.0 130-134 36.021100000000004 37.0 37.0 37.0 37.0 37.0 135-139 36.017399999999995 37.0 37.0 37.0 37.0 37.0 140-144 35.8402 37.0 37.0 37.0 37.0 37.0 145-149 35.679 37.0 37.0 37.0 37.0 37.0 150-151 35.3875 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 1.0 22 1.0 23 3.0 24 1.0 25 1.0 26 4.0 27 9.0 28 8.0 29 24.0 30 22.0 31 37.0 32 54.0 33 63.0 34 102.0 35 335.0 36 2957.0 37 377.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.25 12.25 8.6 42.9 2 19.126506024096386 13.629518072289157 38.880522088353416 28.363453815261042 3 17.2 16.75 26.35 39.7 4 20.1 26.400000000000002 24.7 28.799999999999997 5 24.099999999999998 31.4 25.174999999999997 19.325 6 20.549999999999997 34.175 23.75 21.525 7 15.625 28.499999999999996 38.425 17.45 8 16.075 27.975 31.75 24.2 9 17.375 25.35 34.025 23.25 10-14 20.235 29.025000000000002 27.384999999999998 23.355 15-19 20.26 28.685 26.834999999999997 24.22 20-24 19.915 28.1 28.265 23.72 25-29 19.759999999999998 28.49 27.96 23.79 30-34 19.925 27.825 28.01 24.240000000000002 35-39 20.380000000000003 28.799999999999997 27.435 23.385 40-44 20.125 28.59 27.625 23.66 45-49 20.22 28.865000000000002 27.57 23.345 50-54 20.06 29.235 26.72 23.985 55-59 19.78 28.765 27.265 24.19 60-64 20.635 28.585 26.745 24.035 65-69 19.935 28.084999999999997 27.779999999999998 24.2 70-74 19.97 28.904999999999998 27.485 23.64 75-79 20.595 27.834999999999997 27.650000000000002 23.919999999999998 80-84 20.69 27.935 27.495000000000005 23.880000000000003 85-89 20.505000000000003 28.050000000000004 27.315 24.13 90-94 21.115000000000002 28.62 26.52 23.745 95-99 20.724999999999998 29.065 26.540000000000003 23.669999999999998 100-104 20.76 27.779999999999998 27.12 24.34 105-109 21.375 28.685 26.584999999999997 23.355 110-114 21.525 28.475 26.93 23.07 115-119 21.075 29.81 25.96 23.155 120-124 20.84 27.61 26.810000000000002 24.740000000000002 125-129 21.665 29.03 25.840000000000003 23.465 130-134 21.52 27.944999999999997 26.245 24.29 135-139 21.16 28.799999999999997 25.835 24.205 140-144 21.349999999999998 28.410000000000004 26.334999999999997 23.905 145-149 21.685 28.125 26.215 23.974999999999998 150-151 21.55 27.487499999999997 26.3625 24.6 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.5 2 1.0 3 0.5 4 0.0 5 0.0 6 0.5 7 1.0 8 0.5 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 0.5 19 0.0 20 0.5 21 1.0 22 0.5 23 2.0 24 2.5 25 1.5 26 1.5 27 4.0 28 9.0 29 14.0 30 18.5 31 21.0 32 23.5 33 37.5 34 66.5 35 81.0 36 84.0 37 109.5 38 133.5 39 150.5 40 166.5 41 184.5 42 228.5 43 244.5 44 233.0 45 255.5 46 261.0 47 266.0 48 263.5 49 206.5 50 171.0 51 167.5 52 139.5 53 97.0 54 90.0 55 76.0 56 48.0 57 40.0 58 29.5 59 20.0 60 15.0 61 8.0 62 3.5 63 2.0 64 5.5 65 5.5 66 1.5 67 1.5 68 0.5 69 0.0 70 0.5 71 0.5 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.575 #Duplication Level Percentage of deduplicated Percentage of total 1 71.05359095880422 48.725 2 18.374043018592783 25.2 3 6.598614655486694 13.575000000000001 4 2.3696682464454977 6.5 5 0.9478672985781991 3.25 6 0.5833029529711994 2.4 7 0.07291286912139992 0.35000000000000003 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGTGAGATCTGGAAGGTAAGAAAGAGTCTCGAACTTCTTCAATCCAGTT 7 0.17500000000000002 No Hit GGCAGCTTCTGCTAGTGGTGCACTAACAACCATCGCTACAAGCATGGCAC 7 0.17500000000000002 No Hit CCCAATAAAAAAGGAAGAGGTAAAGCATTTTGCCAAGGTCTAAGTACAAT 6 0.15 No Hit CGCTAAAGGCATGATGACCACACTCATGAGCTATAACCCAAACGCCGGTG 6 0.15 No Hit TTCAGTTTAAACAAATGCAACACGTACAGTACCTCTCTTCACAAATGAAA 6 0.15 No Hit GGTGCTACCTGGGAACCAGAGGGGTCTATCAGGGTCAGCTGCAACACAAA 6 0.15 No Hit CCTTATTTGGAGCTCCACTATACTCTGCATAGTTGCCAAGCTCTGATGCA 6 0.15 No Hit CCCTATTCTACACTTCTCATTTGCCCAGCACCTCAACTTGAGTAATTACA 6 0.15 No Hit GTTCAAGTAAACATAGAGTTCTCCCTTCTACACCTATTAACTTTTATCCC 6 0.15 No Hit CTCCAAACTTACCCGGGCAGGACAAGACAGCACAGCAGAGTCCGTGATAC 6 0.15 No Hit CAATGATTCTGTGCTAGGGACTGCTTCTGGTTGATTGGAATTTATAGCTT 6 0.15 No Hit GTAAGCTTGTGATTGCCCCTTGTGGTTGCAGAGCAATTTTGGGCAGCTCC 6 0.15 No Hit CTCAGGTGCATGCTTACTTCTCAGAAGAGTTTGGTCCACGCTTCTTCCCA 6 0.15 No Hit AAGCCTCTTTGAATTCCGCTTCTGGTCTCACATCAAGAATCACAAAGTTG 6 0.15 No Hit GTCACAGCCTAAATGAAGATAGTCGATACCACGAGCCACTCCAAGGGAAA 6 0.15 No Hit GTTAGGGAAGTATTTTGTGAATTGAAGTGACAAAGAATCATCACAAACAA 6 0.15 No Hit CTCCTGAATAACAATGGCCCTTAAGGAGAGATAATCAGCGACAGATCTAC 6 0.15 No Hit CACACAGATTATTTTGAGCAGAACAGACAGAATCTGAGGATTGAAGTCCA 6 0.15 No Hit GGCCCATCTGCTGTGGATCACCTCAAGCTCACGGTTCTTGGCAAATGTCT 5 0.125 No Hit GCCTTTTTCACCCGAATGATTCCATCCTCGTCCCTCTCAAGCTTCACTTC 5 0.125 No Hit GTGAGATTATTAGGAACCAACTTAATAGCATTACGAGATTATGAGGATTT 5 0.125 No Hit GGAGGTCCAGGAGGTGGAGGTGGATGGTGGTGATGATGGGGTGGTTGATG 5 0.125 No Hit GGTCAGCGTTATCTCTCACCCGCACACTCACCTGTCCAGTTTTGGATTCC 5 0.125 No Hit CTCGAATCTCCCTCTTGCCATCTTGAAAGTTAATACCAAATTTGGTAGCT 5 0.125 No Hit ATCTGTAGGTCTCCAAACTTCAGGACTCTTGTTTCTTTCTTCGGTTCTTC 5 0.125 No Hit AGTCAAAGAGTGCAGCCTTTTCTATTTCTTCAGCCAAAGAAGGTGGTGCG 5 0.125 No Hit CTTGTGATGTGTAGCAACAACTATAAGCTGCAATCTCGGATAAAGAGGAA 5 0.125 No Hit TTTTTAAAATATAAAAAAGAAACAGAAAAATCTTTATTTAATCACAGCAA 5 0.125 No Hit GTTGATTCTCGAGGTTCTGATGTATTCTAAGATATCATTCTCATCACAGA 5 0.125 No Hit GCCATATAGAAACCTCTCACTTGCACCTTCCCCAGCAAAGACTCGGCTTG 5 0.125 No Hit CAGGGATGCTGCTTGGGAAGGTGAGATCTTTGTGCAAAATGTTGGCAAAT 5 0.125 No Hit CTGTAGCCCAAACAGAACTGGAATCAGAGGAATTTAGAATCCAACTCTCT 5 0.125 No Hit GTCAAAGAGTGCAGCCTTTTCTATTTCTTCAGCCAAAGAAGGTGGTGCGA 5 0.125 No Hit CTCATATAAGAGTTGGTTCCAAGTATCAGGCAATCCAGGCAGGCACCAAT 5 0.125 No Hit CTTGGCGGCAATAAAGCTGATGCACTGCACTTGACGCGTGTTGTCGAATC 5 0.125 No Hit GACCACAATGTATTCCACCTTCAGATGCAAAACTGCATACTCTACTGCTG 5 0.125 No Hit TTTCTCAATTAAACACACAGATTTCTTCTCGTCGCACTCATTTTCAATAC 5 0.125 No Hit TGTCTGTCAAGGTACTTCCACTTCCATCTGGTACCACTCCAACATCAAGA 5 0.125 No Hit GGCAGCTTCCTTCAATGCCTCACTCATTGTTGGATGAGCGTGGCATACAC 5 0.125 No Hit CCAGTTGGAGGCCACACCTGCATGCATTGAACTCTTCCGCCATTGCTTGC 5 0.125 No Hit GTCTTAGTAGATTCCCGGAACAATCCGGTAGCGGACCCTCTCACAGTACA 5 0.125 No Hit CGGGAGATTAGAGCATTCTTCTACAATCTGTCTATTGGAAAACAGTAAAG 5 0.125 No Hit CCTGGCACCCTCCCTGCCTCAATTGCCGCAGCCAAGTCCTTTGGCAAGCT 5 0.125 No Hit TTCATAGTTGTTCTCAATCACGGGATCCAGAATCATGCTGTAGTTAGATG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.1875 0.0 0.0 0.0 0.0 80-81 0.35 0.0 0.0 0.0 0.0 82-83 0.425 0.0 0.0 0.0 0.0 84-85 0.6 0.0 0.0 0.0 0.0 86-87 0.7375 0.0 0.0 0.0 0.0 88-89 0.8625 0.0 0.0 0.0 0.0 90-91 1.0 0.0 0.0 0.0 0.0 92-93 1.1 0.0 0.0 0.0 0.0 94-95 1.2374999999999998 0.0 0.0 0.0 0.0 96-97 1.5 0.0 0.0 0.0 0.0 98-99 1.8625 0.0 0.0 0.0 0.0 100-101 2.1375 0.0 0.0 0.0 0.0 102-103 2.45 0.0 0.0 0.0 0.0 104-105 3.125 0.0 0.0 0.0 0.0 106-107 3.7125000000000004 0.0 0.0 0.0 0.0 108-109 4.2625 0.0 0.0 0.0 0.0 110-111 4.737500000000001 0.0 0.0 0.0 0.0 112-113 5.074999999999999 0.0 0.0 0.0 0.0 114-115 5.4375 0.0 0.0 0.0 0.0 116-117 5.824999999999999 0.0 0.0 0.0 0.0 118-119 6.3375 0.0 0.0 0.0 0.0 120-121 7.025 0.0 0.0 0.0 0.0 122-123 7.574999999999999 0.0 0.0 0.0 0.0 124-125 8.25 0.0 0.0 0.0 0.0 126-127 8.962499999999999 0.0 0.0 0.0 0.0 128-129 9.8 0.0 0.0 0.0 0.0 130-131 10.1125 0.0 0.0 0.0 0.0 132-133 10.7625 0.0 0.0 0.0 0.0 134-135 11.4375 0.0 0.0 0.0 0.0 136-137 12.037500000000001 0.0 0.0 0.0 0.0 138-139 12.787500000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCACAG 10 0.006830828 145.0 1 GAAATCA 10 0.006830828 145.0 5 GATGACA 40 0.0076550315 18.125 140-144 >>END_MODULE SRR13695425 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695425_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.29175 37.0 37.0 37.0 37.0 37.0 2 36.2295 37.0 37.0 37.0 37.0 37.0 3 36.15 37.0 37.0 37.0 37.0 37.0 4 36.237 37.0 37.0 37.0 37.0 37.0 5 36.369 37.0 37.0 37.0 37.0 37.0 6 36.3345 37.0 37.0 37.0 37.0 37.0 7 36.3085 37.0 37.0 37.0 37.0 37.0 8 36.3885 37.0 37.0 37.0 37.0 37.0 9 36.353 37.0 37.0 37.0 37.0 37.0 10-14 36.338800000000006 37.0 37.0 37.0 37.0 37.0 15-19 36.3227 37.0 37.0 37.0 37.0 37.0 20-24 36.2911 37.0 37.0 37.0 37.0 37.0 25-29 36.23755 37.0 37.0 37.0 37.0 37.0 30-34 36.2014 37.0 37.0 37.0 37.0 37.0 35-39 36.16555 37.0 37.0 37.0 37.0 37.0 40-44 36.171 37.0 37.0 37.0 37.0 37.0 45-49 36.1548 37.0 37.0 37.0 37.0 37.0 50-54 36.105500000000006 37.0 37.0 37.0 37.0 37.0 55-59 36.124849999999995 37.0 37.0 37.0 37.0 37.0 60-64 36.10295 37.0 37.0 37.0 37.0 37.0 65-69 36.06015000000001 37.0 37.0 37.0 37.0 37.0 70-74 36.01545 37.0 37.0 37.0 37.0 37.0 75-79 36.0099 37.0 37.0 37.0 37.0 37.0 80-84 35.9725 37.0 37.0 37.0 37.0 37.0 85-89 35.962599999999995 37.0 37.0 37.0 37.0 37.0 90-94 35.89295 37.0 37.0 37.0 37.0 37.0 95-99 35.936099999999996 37.0 37.0 37.0 37.0 37.0 100-104 35.87485 37.0 37.0 37.0 37.0 37.0 105-109 35.876599999999996 37.0 37.0 37.0 37.0 37.0 110-114 35.789249999999996 37.0 37.0 37.0 37.0 37.0 115-119 35.7337 37.0 37.0 37.0 37.0 37.0 120-124 35.689550000000004 37.0 37.0 37.0 37.0 37.0 125-129 35.6216 37.0 37.0 37.0 37.0 37.0 130-134 35.4641 37.0 37.0 37.0 37.0 37.0 135-139 35.49405 37.0 37.0 37.0 37.0 37.0 140-144 35.32925 37.0 37.0 37.0 34.6 37.0 145-149 35.070049999999995 37.0 37.0 37.0 27.4 37.0 150-151 34.881249999999994 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 1.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 2.0 15 3.0 16 1.0 17 1.0 18 1.0 19 0.0 20 1.0 21 6.0 22 5.0 23 7.0 24 1.0 25 9.0 26 5.0 27 5.0 28 14.0 29 23.0 30 21.0 31 33.0 32 48.0 33 101.0 34 205.0 35 534.0 36 2734.0 37 238.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.742792679869645 24.918525946352467 11.356229631486588 29.9824517422913 2 26.625 25.0 32.6 15.775 3 21.15 26.25 31.775 20.825 4 22.575 33.85 24.275 19.3 5 26.174999999999997 33.5 22.900000000000002 17.424999999999997 6 19.75 40.825 21.349999999999998 18.075 7 20.5 22.85 36.725 19.925 8 19.975 27.1 28.625 24.3 9 21.725 25.7 30.975 21.6 10-14 22.39 29.68 26.46 21.47 15-19 23.26 28.365000000000002 27.355 21.02 20-24 22.821410705352676 28.644322161080538 27.368684342171086 21.1655827913957 25-29 22.797097823367526 28.541406054540907 27.74080560420315 20.920690517888417 30-34 22.693616169701823 27.541524914948965 27.581548929357613 22.183309985991595 35-39 23.242783530942017 27.645204862674472 27.62019110510781 21.4918205012757 40-44 22.503502101260757 27.856714028417052 28.34700820492295 21.292775665399237 45-49 22.671335667833915 28.029014507253624 28.039019509754876 21.260630315157577 50-54 22.471235617808905 28.224112056028016 28.059029514757377 21.245622811405703 55-59 23.45258944208156 27.425569176882664 28.20115086314736 20.920690517888417 60-64 23.33783580969533 26.994847165941266 28.360598329080993 21.306718695282406 65-69 22.80570142535634 27.66191547886972 27.89197299324831 21.64041010252563 70-74 22.677007755816863 27.385539154365773 28.19614711033275 21.74130597948461 75-79 23.864318591154692 27.496497898739243 28.02181308785271 20.61737042225335 80-84 23.936968484242122 27.313656828414207 27.613806903451728 21.135567783891947 85-89 23.58150705493846 27.539277494245972 26.95386770739518 21.925347743420396 90-94 23.42288258542198 27.500125068787835 27.430086547601178 21.646905798189003 95-99 23.25662831415708 28.369184592296147 27.18359179589795 21.190595297648823 100-104 23.427570678008504 27.6257192894671 27.090317738303725 21.856392294220665 105-109 23.216251375963175 27.489242469728808 28.314820374261984 20.979685780046033 110-114 24.31837510630847 28.29556255940767 26.944819650807943 20.441242683475913 115-119 24.594756854112468 28.412047228337002 27.04622773664199 19.946968180908545 120-124 25.17888416312234 27.410557918438826 26.810107580685518 20.600450337753315 125-129 25.52031218731239 27.556533920352212 26.776065639383628 20.147088252951768 130-134 25.813069148403883 27.549284499149408 26.463524467126987 20.174121885319725 135-139 27.225419064298222 27.430572929697274 25.789342006504878 19.554665999499623 140-144 27.159937965881237 28.125469007954372 25.008754815148333 19.705838211016058 145-149 27.615711783837877 27.820865649236925 25.509131848886664 19.05429071803853 150-151 28.646484863647736 26.732549412059043 25.856892669502123 18.764073054791094 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.5 9 0.5 10 1.0 11 1.5 12 0.5 13 0.0 14 0.0 15 0.0 16 0.5 17 1.0 18 0.5 19 0.0 20 0.0 21 0.5 22 1.5 23 2.0 24 2.5 25 3.5 26 2.5 27 3.0 28 4.5 29 5.5 30 7.5 31 18.5 32 27.5 33 31.5 34 45.0 35 63.0 36 80.5 37 100.5 38 122.5 39 153.0 40 212.5 41 247.0 42 246.5 43 271.0 44 297.5 45 286.0 46 253.5 47 230.0 48 214.0 49 194.0 50 184.0 51 157.5 52 115.0 53 91.5 54 70.0 55 61.0 56 48.0 57 32.5 58 22.0 59 21.0 60 21.5 61 11.0 62 10.0 63 6.5 64 2.0 65 1.5 66 2.0 67 1.0 68 0.0 69 1.0 70 1.0 71 0.5 72 0.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27499999999999997 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.05 25-29 0.075 30-34 0.06 35-39 0.055 40-44 0.06 45-49 0.05 50-54 0.05 55-59 0.075 60-64 0.055 65-69 0.025 70-74 0.075 75-79 0.06 80-84 0.05 85-89 0.06999999999999999 90-94 0.055 95-99 0.05 100-104 0.075 105-109 0.06999999999999999 110-114 0.055 115-119 0.06 120-124 0.075 125-129 0.06 130-134 0.06999999999999999 135-139 0.075 140-144 0.055 145-149 0.075 150-151 0.075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.625 #Duplication Level Percentage of deduplicated Percentage of total 1 71.62112932604737 49.15 2 17.88706739526412 24.55 3 6.33879781420765 13.05 4 2.4043715846994536 6.6000000000000005 5 1.0564663023679417 3.6249999999999996 6 0.5828779599271403 2.4 7 0.03642987249544627 0.17500000000000002 8 0.03642987249544627 0.2 9 0.0 0.0 >10 0.03642987249544627 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATGGCCTCTTCCAAACCTCTCATCACCCTCTCCTCCTCTTCCCTCCCCAC 10 0.25 No Hit CAGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 8 0.2 No Hit AGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATC 7 0.17500000000000002 No Hit GGTAGGCAAAGAAGAAGAATATCTAACAATCATGGGCAAGGTACACGGAT 6 0.15 No Hit GGATTTTGGGAAAAGGAGAAGCGAGGCTTCAAGAGAAGGAGAGTCACTCC 6 0.15 No Hit AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 6 0.15 No Hit ATTCATATTCATTTTGTCATGTTAGTTAATTATCATGAAGCTTCACATAT 6 0.15 No Hit TGAAGTTAAGGTTTGCAATATTCTCGGAGAGTTTGGATTTGATTAGATCC 6 0.15 No Hit GAAAGAGATGGCAGCAGCAGTGAAAAGGATCAAGCTGGGATCACAGGGCC 6 0.15 No Hit CTGGAAGACAAGCTGTAGGGATTAATATGCATGGATGGTTAACTCTGCCC 6 0.15 No Hit AGTGTCCCAGAAAACTTTTGAGAAATCAAGATTTTTAGGAGAGCAAAATC 6 0.15 No Hit CGCTACTGGACTATGTGGAAGCTACCCATGTTTGGATGCACTGAGGCATC 6 0.15 No Hit GCAAAAACCAGAACAAAAAACCTCTAGAATGGCCACTGTCACCTCTGCTG 6 0.15 No Hit ACTGAAACTGAGTAAACTTAAAGAGCGAGACAGAGTGAGGCATGGCAGAA 6 0.15 No Hit AAGTTCATCTTTGAAATTCACTGTTTTGAACCCAAAGGGACGTATCTGGA 6 0.15 No Hit AAGCCTCTTTGTATCCTAATTATCAATCATCGCCACTAATTTTCTCTTCA 6 0.15 No Hit CAATGATGAAGATGAAGATGACGAGGAGGGCAGTGCAGAGGAGGATGACG 6 0.15 No Hit TTTGAACATGATGAGGAAGTTCACTGGTGGAAGTGAACTGATCAGACCAA 6 0.15 No Hit ATTATGTGCTTGGATGTTCAAATGTGCCCTTGCCTTGATTGTACAATTGG 6 0.15 No Hit AGCGTACCTTGTTTGTCTTCCTTGTTTGTTTTGCTTGATCTGGCTTTATG 5 0.125 No Hit ACCTCCTTTGGAACCCGCAGCTATAGCAGCAAATTGAGAATCCCCAGTTT 5 0.125 No Hit GTTGGAATATTCAGCTGAGGATGAAGCCAGAAGTGAAGCACCTGTTATGA 5 0.125 No Hit AAACTGTAGCAGCTGCGAAAGTGGAGCAGATAACAGCTGAATTACAAACC 5 0.125 No Hit ATCGGATTCGACAACACGCGTCAAGTGCAGTGCATCAGCTTTATTGCCGC 5 0.125 No Hit AGAGGGTGTAATAGCTAAGTAGCCTGTAAGAGATGGCTTCCTCTGTGATT 5 0.125 No Hit GTGATATTTCTAACCATCCTTCTGTTTGACCGAGCTAAAAGGGATGATGA 5 0.125 No Hit AGCAGGTCAAGGCACTTGGTGTTGAGTACTGTGTTGGGAAGTTCCCTCTC 5 0.125 No Hit GTGGTGGGAATGATGATGGTGGTGATGCTGGATCCCGCAACAAGAGTGAG 5 0.125 No Hit GCCTAATGGATCTCTTGATAGACACTTGTTTTCTCGAGAAGGATCAATCT 5 0.125 No Hit CTTCGGAAGAAAATCTTTTGGCCACAAAAATGGCTTCTGTTTGTGCTTCT 5 0.125 No Hit CAAAGTAACAAACTAGGATTTCAAGCTCCTGCTTTGGTCAATGATCATTG 5 0.125 No Hit CTCTGAGCAAACCCCATCATACCTGACCGGTGAATTCCCTGGTGATTATG 5 0.125 No Hit GCTCTGTGTTACTCGGGTGTTAATTAAAATACCCGTTTCTCAAATTTCGC 5 0.125 No Hit GTTCCAGTCCATGGGACAGTATGGTCAGGGTGAACTGCAAAAGATTGCAA 5 0.125 No Hit CTCGCATTGGTTTTTTGAGAAAGGCTATTGAATGTAAGGAGTCACGGATA 5 0.125 No Hit CAGTGAAGGGTGGACAGAATCTGGAACTGGATCGTCTTTTAGGAGGAACT 5 0.125 No Hit GGTCTACATATCCGGCAGGTGCACCTCCGGCGGTTGGTGTTGTAAGCAAA 5 0.125 No Hit CACCATTCAACGGCCTCAAGTCTACCTCAGCTTTCCCGGTCACCAGAAAG 5 0.125 No Hit GTTCTAATGAGTTTCTTGACTTAAGGAAATAATCACAGGCATGGGCCACG 5 0.125 No Hit CTGTGTCTTGAAGTTGAGAGGTTAGGGTTGAGTGCGGTTATTATGGGGAG 5 0.125 No Hit CTGTAAGCTTTTTCAAAGTATTACTTCCTTTCTCTATAAATCAATGGCTC 5 0.125 No Hit ATCAAACATCATAACCAAACTCTCAGTTTCAACACAAGAAGAAGAAGTGG 5 0.125 No Hit TTTTTTTTTCCTACTAAATCTATTGCGATTGGCTTTGGAGTTTGGGGCTT 5 0.125 No Hit GGGTCTGGCAAGAAGGAGATCGTGTAGGAAGAGCAATTTATGCATCTCAA 5 0.125 No Hit CAGATCCTTTTGCACCGCCACCTCCTCCTCCACATCGACCCTTTGACAGC 5 0.125 No Hit CAATTTTTCTCCGAAGTGGTGATGTGGTTCTCATGGCTGGAGAAGCACGA 5 0.125 No Hit TACTTTGTTGCCCAACTCTGAGTCATCTCTGTAATTCCGAGAGAACGGCA 5 0.125 No Hit CATACTCTAAAATCTTCTTATAATTCCAGTTGTAATATTCTGCTAGCATA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.1875 0.0 0.0 0.0 0.0 80-81 0.35 0.0 0.0 0.0 0.0 82-83 0.425 0.0 0.0 0.0 0.0 84-85 0.6 0.0 0.0 0.0 0.0 86-87 0.7375 0.0 0.0 0.0 0.0 88-89 0.8875 0.0 0.0 0.0 0.0 90-91 1.025 0.0 0.0 0.0 0.0 92-93 1.125 0.0 0.0 0.0 0.0 94-95 1.2625000000000002 0.0 0.0 0.0 0.0 96-97 1.525 0.0 0.0 0.0 0.0 98-99 1.8875 0.0 0.0 0.0 0.0 100-101 2.175 0.0 0.0 0.0 0.0 102-103 2.5 0.0 0.0 0.0 0.0 104-105 3.1875 0.0 0.0 0.0 0.0 106-107 3.7874999999999996 0.0 0.0 0.0 0.0 108-109 4.3375 0.0 0.0 0.0 0.0 110-111 4.8125 0.0 0.0 0.0 0.0 112-113 5.15 0.0 0.0 0.0 0.0 114-115 5.5125 0.0 0.0 0.0 0.0 116-117 5.9 0.0 0.0 0.0 0.0 118-119 6.4125 0.0 0.0 0.0 0.0 120-121 7.075 0.0 0.0 0.0 0.0 122-123 7.625 0.0 0.0 0.0 0.0 124-125 8.274999999999999 0.0 0.0 0.0 0.0 126-127 8.9875 0.0 0.0 0.0 0.0 128-129 9.8 0.0 0.0 0.0 0.0 130-131 10.1125 0.0 0.0 0.0 0.0 132-133 10.774999999999999 0.0 0.0 0.0 0.0 134-135 11.6 0.0 0.0 0.0 0.0 136-137 12.212499999999999 0.0 0.0 0.0 0.0 138-139 12.9875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATGACGT 20 0.00593511 29.0 140-144 GATGACG 20 0.00593511 29.0 140-144 TGACGTG 20 0.00593511 29.0 140-144 AAGAGTG 40 0.0076550315 18.125 130-134 >>END_MODULE Read 636871 spots for SRR13695425.sra Written 636871 spots for SRR13695425.sra Read 636871 spots for SRR13695425.sra Written 636871 spots for SRR13695425.sra Read 636871 spots for SRR13695425.sra Written 636871 spots for SRR13695425.sra Read 636871 spots for SRR13695425.sra Written 636871 spots for SRR13695425.sra Read 636871 spots for SRR13695425.sra Written 636871 spots for SRR13695425.sra Read 636871 spots for SRR13695425.sra Written 636871 spots for SRR13695425.sra Read 636871 spots for SRR13695425.sra Written 636871 spots for SRR13695425.sra Read 636871 spots for SRR13695425.sra Written 636871 spots for SRR13695425.sra Read 636871 spots for SRR13695425.sra Written 636871 spots for SRR13695425.sra Read 636871 spots for SRR13695425.sra Written 636871 spots for SRR13695425.sra Read 636871 spots for SRR13695425.sra Written 636871 spots for SRR13695425.sra Read 636871 spots for SRR13695425.sra Written 636871 spots for SRR13695425.sra Read 636871 spots for SRR13695425.sra Written 636871 spots for SRR13695425.sra Read 636871 spots for SRR13695425.sra Written 636871 spots for SRR13695425.sra Read 636871 spots for SRR13695425.sra Written 636871 spots for SRR13695425.sra Read 636875 spots for SRR13695425.sra Written 636875 spots for SRR13695425.sra Read 636871 spots for SRR13695425.sra Written 636871 spots for SRR13695425.sra Read 636871 spots for SRR13695425.sra Written 636871 spots for SRR13695425.sra Read 636871 spots for SRR13695425.sra Written 636871 spots for SRR13695425.sra Read 636871 spots for SRR13695425.sra Written 636871 spots for SRR13695425.sra SRR ids: ['SRR13695425.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_6sfubkag SRR13695425.sra spots: 12737424 blocks: [[1, 636871], [636872, 1273742], [1273743, 1910613], [1910614, 2547484], [2547485, 3184355], [3184356, 3821226], [3821227, 4458097], [4458098, 5094968], [5094969, 5731839], [5731840, 6368710], [6368711, 7005581], [7005582, 7642452], [7642453, 8279323], [8279324, 8916194], [8916195, 9553065], [9553066, 10189936], [10189937, 10826807], [10826808, 11463678], [11463679, 12100549], [12100550, 12737424]] SRR13695425 file size 4307033 SRR13695425 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695425 SRR13695425_1.fastq SRR13695425_2.fastq Input file: SRR13695425_1.fastq Paired file: SRR13695425_2.fastq trimmed: SRR13695425-trimmed-pair1.fastq, SRR13695425-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 02:33:16 2025 >> started Wed Feb 12 02:33:30 2025 >> done (13.677s) 12737424 read pairs processed; of these: 104 ( 0.00%) short read pairs filtered out after trimming by size control 934 ( 0.01%) empty read pairs filtered out after trimming by size control 12736386 (99.99%) read pairs available; of these: 2088685 (16.40%) trimmed read pairs available after processing 10647701 (83.60%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 3 0.00% 20 2 0.00% 21 2 0.00% 22 3 0.00% 23 1 0.00% 24 1 0.00% 25 2 0.00% 26 1 0.00% 27 0 0.00% 28 2 0.00% 29 1 0.00% 30 4 0.00% 31 8 0.00% 32 5 0.00% 33 4 0.00% 34 4 0.00% 35 3 0.00% 36 6 0.00% 37 11 0.00% 38 10 0.00% 39 10 0.00% 40 13 0.00% 41 19 0.00% 42 9 0.00% 43 28 0.00% 44 17 0.00% 45 24 0.00% 46 29 0.00% 47 31 0.00% 48 36 0.00% 49 55 0.00% 50 77 0.00% 51 76 0.00% 52 121 0.00% 53 124 0.00% 54 130 0.00% 55 129 0.00% 56 165 0.00% 57 212 0.00% 58 236 0.00% 59 283 0.00% 60 346 0.00% 61 416 0.00% 62 480 0.00% 63 516 0.00% 64 545 0.00% 65 651 0.01% 66 719 0.01% 67 928 0.01% 68 1037 0.01% 69 1177 0.01% 70 1265 0.01% 71 1540 0.01% 72 1829 0.01% 73 2150 0.02% 74 2457 0.02% 75 2568 0.02% 76 2820 0.02% 77 3168 0.02% 78 3642 0.03% 79 3965 0.03% 80 4360 0.03% 81 4936 0.04% 82 5563 0.04% 83 6001 0.05% 84 6799 0.05% 85 7412 0.06% 86 7997 0.06% 87 8575 0.07% 88 9171 0.07% 89 9355 0.07% 90 10456 0.08% 91 11073 0.09% 92 11790 0.09% 93 13044 0.10% 94 13573 0.11% 95 14575 0.11% 96 15467 0.12% 97 16151 0.13% 98 16547 0.13% 99 17455 0.14% 100 18633 0.15% 101 18264 0.14% 102 19346 0.15% 103 20237 0.16% 104 21109 0.17% 105 22107 0.17% 106 23317 0.18% 107 23878 0.19% 108 24446 0.19% 109 25196 0.20% 110 26018 0.20% 111 26381 0.21% 112 27477 0.22% 113 27996 0.22% 114 28342 0.22% 115 29956 0.24% 116 31387 0.25% 117 31751 0.25% 118 32762 0.26% 119 32956 0.26% 120 33914 0.27% 121 34680 0.27% 122 34983 0.27% 123 35491 0.28% 124 36343 0.29% 125 36603 0.29% 126 37792 0.30% 127 38821 0.30% 128 40031 0.31% 129 40234 0.32% 130 40406 0.32% 131 41113 0.32% 132 41747 0.33% 133 41512 0.33% 134 42422 0.33% 135 42720 0.34% 136 43228 0.34% 137 43911 0.34% 138 45340 0.36% 139 45926 0.36% 140 45943 0.36% 141 46916 0.37% 142 47600 0.37% 143 47079 0.37% 144 48076 0.38% 145 48556 0.38% 146 48364 0.38% 147 49856 0.39% 148 50457 0.40% 149 50763 0.40% 150 51881 0.41% 151 10647701 83.60% 12736386 reads passed initial QC criterion=sequence-density sequence-density=0.52 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=24 prefix-density=0.50 prefix-fanout=2.0 sequence=GTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGAGTATGAATGTGTTCTCTGTG criterion=fanout-score sequence-density=0.02 sequence-density-rank=27 fanout-score=117.62 fanout-score-rank=1 prefix-density=0.21 prefix-fanout=12.8 sequence=CTGCTGCTGCATTTATTAGAGAAGGAGATGCTGGTAATCTCCATTTCACAAGTTCATTAATCTCGATCGAAAATATGGCTCATTTAAGCATATACAAAGTACATCTGGGAAAGAAAGTTAAGAACCAAGATAGGGTCACTGATATTTGGATGATGTTCATACGGAGAGGGAGGGAGAAAGGCTGTACTCAAGCCCCCAGAGCTCAAAACTGCCTTTGACAAAATCATAATAACCACCCTTCAGTCCTAGAGTTTTGTTCACCAAGCCATCTCTCACAAACGGGTAGGTTAGCAAGTGTCCAAGGGACACGTTCACTGCCTCCTTTTCACATTGTGTACAGAGGTCTGGGAAAGGTGCATTGGCATGTTCTGCTAAAACCTTAGTCTTGGCAGGGTA criterion=sequence-density sequence-density=0.56 sequence-density-rank=1 fanout-score=2.05 fanout-score-rank=27 prefix-density=0.56 prefix-fanout=2.0 sequence=TGTAAGAGATGGCTTCCTC criterion=fanout-score sequence-density=0.11 sequence-density-rank=23 fanout-score=41.48 fanout-score-rank=1 prefix-density=0.35 prefix-fanout=13.6 sequence=AAAGAAAAGAAAA SRR13695425 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 02:34:24 Started mapping on | Feb 12 02:34:24 Finished on | Feb 12 02:35:27 Mapping speed, Million of reads per hour | 727.79 Number of input reads | 12736386 Average input read length | 285 UNIQUE READS: Uniquely mapped reads number | 10645294 Uniquely mapped reads % | 83.58% Average mapped length | 286.82 Number of splices: Total | 10619237 Number of splices: Annotated (sjdb) | 10405231 Number of splices: GT/AG | 10404320 Number of splices: GC/AG | 174165 Number of splices: AT/AC | 5811 Number of splices: Non-canonical | 34941 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 2.89 Insertion rate per base | 0.02% Insertion average length | 2.28 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 244142 % of reads mapped to multiple loci | 1.92% Number of reads mapped to too many loci | 19595 % of reads mapped to too many loci | 0.15% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 14.26% % of reads unmapped: other | 0.08% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1847076 1847076 1847076 N_multimapping 244142 244142 244142 N_noFeature 308851 10423339 412761 N_ambiguous 230882 2684 110735 UnstrandedReadsAssigned:10105561 PositiveStrandReadsAssigned:219271 NegativeStrandReadsAssigned:10121798 Dataset is classified negative stranded MeadianReadLen=143 20thPercentileLength=143 echo kmer=139 SRR13695425 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695425-trimmed-pair1.fastq SRR13695425-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 12,736,386 reads, 11,550,637 reads pseudoaligned [quant] estimated average fragment length: 225.005 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,113 rounds 52401 SRR13695425.ke.tsv 34699 SRR13695425.se.tsv 87100 total ==> SRR13695425.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1794 309 13.085 Potri.005G024800.1.v4.1 1035 810.995 223 20.8893 Potri.004G059700.1.v4.1 961 737.059 1 0.103071 Potri.007G009000.2.v4.1 1416 1192 0 0 Potri.003G141000.2.v4.1 2943 2719 861 24.0565 Potri.016G087400.1.v4.1 270 97.7842 600 466.144 Potri.015G069301.1.v4.1 564 346.729 0 0 Potri.010G195200.1.v4.1 1773 1549 54 2.64838 Potri.012G127500.1.v4.1 977 753.038 85 8.5751 ==> SRR13695425.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 171 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 191 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 1 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 5 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR13695425 completed mapping pipeline successfully