Starting /dee2/code/volunteer_pipeline.sh SRR13695426 current disk space = 3050780172288 free memory = 1462407676 SRR13695426 SRAfilesize 4da5f17b60ca2714f0afa7c74db23493 SRR13695426.sra SRR13695426.sra file validated SRR13695426 is paired end SRR13695426 is conventional basespace SRR13695426 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695426_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5905 37.0 37.0 37.0 37.0 37.0 2 36.32025 37.0 37.0 37.0 37.0 37.0 3 36.549 37.0 37.0 37.0 37.0 37.0 4 36.6575 37.0 37.0 37.0 37.0 37.0 5 36.6355 37.0 37.0 37.0 37.0 37.0 6 36.6705 37.0 37.0 37.0 37.0 37.0 7 36.5315 37.0 37.0 37.0 37.0 37.0 8 36.5425 37.0 37.0 37.0 37.0 37.0 9 36.5865 37.0 37.0 37.0 37.0 37.0 10-14 36.5512 37.0 37.0 37.0 37.0 37.0 15-19 36.5238 37.0 37.0 37.0 37.0 37.0 20-24 36.494299999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.4205 37.0 37.0 37.0 37.0 37.0 30-34 36.4096 37.0 37.0 37.0 37.0 37.0 35-39 36.3728 37.0 37.0 37.0 37.0 37.0 40-44 36.3876 37.0 37.0 37.0 37.0 37.0 45-49 36.3829 37.0 37.0 37.0 37.0 37.0 50-54 36.3552 37.0 37.0 37.0 37.0 37.0 55-59 36.34160000000001 37.0 37.0 37.0 37.0 37.0 60-64 36.3244 37.0 37.0 37.0 37.0 37.0 65-69 36.191 37.0 37.0 37.0 37.0 37.0 70-74 36.19840000000001 37.0 37.0 37.0 37.0 37.0 75-79 36.1821 37.0 37.0 37.0 37.0 37.0 80-84 36.158500000000004 37.0 37.0 37.0 37.0 37.0 85-89 36.158 37.0 37.0 37.0 37.0 37.0 90-94 36.0576 37.0 37.0 37.0 37.0 37.0 95-99 36.109300000000005 37.0 37.0 37.0 37.0 37.0 100-104 36.0489 37.0 37.0 37.0 37.0 37.0 105-109 36.01109999999999 37.0 37.0 37.0 37.0 37.0 110-114 35.99399999999999 37.0 37.0 37.0 37.0 37.0 115-119 36.0285 37.0 37.0 37.0 37.0 37.0 120-124 35.9727 37.0 37.0 37.0 37.0 37.0 125-129 35.9402 37.0 37.0 37.0 37.0 37.0 130-134 35.9465 37.0 37.0 37.0 37.0 37.0 135-139 35.854600000000005 37.0 37.0 37.0 37.0 37.0 140-144 35.739999999999995 37.0 37.0 37.0 37.0 37.0 145-149 35.5637 37.0 37.0 37.0 37.0 37.0 150-151 35.3395 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 2.0 19 1.0 20 2.0 21 1.0 22 1.0 23 2.0 24 2.0 25 5.0 26 11.0 27 9.0 28 11.0 29 20.0 30 31.0 31 27.0 32 57.0 33 80.0 34 128.0 35 294.0 36 2962.0 37 354.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.324999999999996 11.899999999999999 11.1 37.675 2 21.146592909228062 13.024893135529295 35.051546391752574 30.776967563490064 3 18.875 17.474999999999998 27.150000000000002 36.5 4 23.525 24.474999999999998 24.0 28.000000000000004 5 24.0 30.725 23.825 21.45 6 21.525 33.6 24.5 20.375 7 17.125 28.375 39.45 15.049999999999999 8 17.724999999999998 24.975 33.525 23.775 9 17.150000000000002 26.8 34.125 21.925 10-14 19.71 29.470000000000002 28.285 22.535 15-19 20.13 28.389999999999997 28.24 23.24 20-24 19.875 28.71 28.444999999999997 22.97 25-29 20.19 29.73 26.595000000000002 23.485 30-34 19.755 28.810000000000002 27.605 23.830000000000002 35-39 20.044999999999998 29.035 27.35 23.57 40-44 20.165 28.915000000000003 27.389999999999997 23.53 45-49 20.380000000000003 28.34 28.110000000000003 23.169999999999998 50-54 20.43 29.555 26.775 23.24 55-59 20.62 29.580000000000002 26.955000000000002 22.845 60-64 20.365 28.689999999999998 27.400000000000002 23.544999999999998 65-69 20.255000000000003 28.575 27.6 23.57 70-74 20.575 28.965000000000003 26.86 23.599999999999998 75-79 19.86 28.42 27.37 24.349999999999998 80-84 20.02 27.755000000000003 28.235 23.990000000000002 85-89 20.875 28.860000000000003 27.089999999999996 23.175 90-94 20.825 28.005000000000003 26.795 24.375 95-99 21.029999999999998 27.794999999999998 27.384999999999998 23.79 100-104 20.669999999999998 29.38 26.795 23.155 105-109 21.21 28.89 27.155 22.745 110-114 20.905 28.13 26.985 23.98 115-119 21.92 28.384999999999998 26.545 23.150000000000002 120-124 21.060000000000002 27.650000000000002 27.37 23.919999999999998 125-129 21.44 28.235 26.384999999999998 23.94 130-134 21.67 28.74 26.474999999999998 23.115 135-139 21.475 28.845 25.974999999999998 23.705000000000002 140-144 20.79 27.79 27.634999999999998 23.785 145-149 20.84 28.744999999999997 25.825 24.59 150-151 20.8875 27.8875 26.450000000000003 24.775 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 1.0 2 1.0 3 0.5 4 0.5 5 0.0 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.5 12 0.5 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 1.5 22 2.0 23 0.5 24 3.5 25 4.5 26 9.0 27 10.0 28 8.0 29 12.0 30 17.5 31 24.0 32 37.0 33 48.0 34 52.5 35 65.0 36 82.0 37 99.5 38 142.0 39 170.0 40 170.0 41 203.0 42 211.5 43 238.0 44 269.0 45 264.5 46 257.0 47 252.0 48 261.5 49 234.0 50 186.0 51 145.0 52 118.0 53 96.0 54 74.5 55 58.5 56 45.5 57 31.5 58 21.5 59 22.5 60 18.0 61 9.5 62 4.5 63 2.5 64 1.5 65 1.0 66 3.5 67 4.0 68 1.5 69 0.0 70 0.0 71 0.5 72 0.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.575 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.25 #Duplication Level Percentage of deduplicated Percentage of total 1 68.86792452830188 45.625 2 19.622641509433965 26.0 3 6.566037735849056 13.05 4 2.8679245283018866 7.6 5 1.2075471698113207 4.0 6 0.6037735849056604 2.4 7 0.1509433962264151 0.7000000000000001 8 0.07547169811320754 0.4 9 0.03773584905660377 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTCGTTTTAAAACTTTCAAGAGCTTTAGAGCGTAAATGCCCCAACATGGT 9 0.22499999999999998 No Hit CTCATGGCTCCGTGTGACGTTGCTGAGTGGACATAGAACCGGTTCGGCCG 8 0.2 No Hit CTCTCTGTCACTTGAAGCTCTATTTCCTTGGCCTTCAACCTCTCAAAGAC 8 0.2 No Hit GTCCATGAATAAGATGAGAGTAGGCTTCCTTGATGCCAAATCACATTGTT 7 0.17500000000000002 No Hit CTCATTTCCAAGACCAACTATGAATTCCACTCCTGTATTAGCAAATGCTT 7 0.17500000000000002 No Hit GTAGGAGCATGATAGAGGTACGAGTATAACTCAAAGGTTCTTCCATGATA 7 0.17500000000000002 No Hit CTCAAAGTCTCCTTCTCCCTCTTCAATCTCGTTGCTGTATCCTCCAATTC 7 0.17500000000000002 No Hit TCTACCTTTTCCTTGTGAAGCTTCAACCTTTTTTTCTTCTTTGACTGCAG 6 0.15 No Hit ATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATA 6 0.15 No Hit TCAGCATAGGAGAAGATTCGAGTCTGGAGTAGCTTGTCATCTGAATAGTA 6 0.15 No Hit CCGCTGAATTAATTTACATATTAGCACGCAGTTCATATTTCAACCAACAC 6 0.15 No Hit CCACATTTGCAGCCATTCTCAGCACCAAAGTTCATCTCAGAGCTCTCGTA 6 0.15 No Hit ATTCCCAACTCGTGCAATTGTATCAGCAGCAGGAGTGCCAAGCAAATCAG 6 0.15 No Hit CTCCTTCAGTGCCCACTCATCCACTCCATGCCACGGGTTGTTCTCAGGGC 6 0.15 No Hit CTCCCAGCAGCCTTAACAGCTGACTCACTACCAAGCTCCTTCAGATCATC 6 0.15 No Hit CTCCAGATAACCTTGCTATTCTTTCATTCAATATTTTCTTTTGAAATTTT 6 0.15 No Hit CTATCTAAATTCTACCCCTAAAAAGAGAGTAAATAATAAAATACAAGGAC 6 0.15 No Hit GGGCTGGTCAAGATATTGTTGTGGACAGGATCGGCCAGGTGATCCAGGAG 6 0.15 No Hit GTCCCTTTGGTTTCAGCCTTCATCTTCACTGCCAACTTCACCGAAGCATC 6 0.15 No Hit CCTCAAGAAGTTGGCGGAAGTCCAGTAACACATTCACGCTCCCTTGAAGA 6 0.15 No Hit AGCCAATAGCTGATCATCGTAAAACTCGAACAGAAAAAATAAGATACCAA 6 0.15 No Hit TGGTAATCTGCTGGAGCTCCTCCCTTCTCACCACCTTCACCCCCTCGAGG 6 0.15 No Hit CAAAATACAATCTTTATCGTTATACCAAACACCCCCCTTCAATCTTTCAA 6 0.15 No Hit TTCAGGGTAGACGTATGACTTTCCATCAGAATATATCACTTTAACGGGTT 5 0.125 No Hit TGGAAGATGACAATCTTCTTCGTTCCTTGCATAAAAAAGGAAGCTGTGTA 5 0.125 No Hit CTCAAATTGGTTACCAAGGTGTGCTTATCCAAAGGCAAAGACCGAGCCAT 5 0.125 No Hit TGTCTTATAAGTTTGTTGTACACAACGCATAGTGGATAAAAATCCTTGTT 5 0.125 No Hit GGCTTCCATCAGCAATTGTTCGGTCACCACAAGAGCACAGTGATCTATAA 5 0.125 No Hit ATCGTACTCTGCAACAACCTTGAAGCTCCCTGGTGAAATTTTGGAGTTTG 5 0.125 No Hit TGAGGAGCTCCCTTGAGGACATTGTTGTGGATATCAGCTTTGCCTTTCTC 5 0.125 No Hit ATACCAATTACACTGTGTTCGATGAAGCAGTTGTTTAAGAAACTTCCATG 5 0.125 No Hit TGGTAAACAAATCCTTTCCAACTTTGCCAAATTGGAGGATGATATTCTCA 5 0.125 No Hit GCCACATGGTAGATTTACAGCTATGAATAAATCTAATGTGCACGATTGCA 5 0.125 No Hit GTCTTGACGATTAGACTGATTGCCTTCTTGCTGTCTGGAGTCATATCTCC 5 0.125 No Hit GAGAAAGACACGTGCCATGGGTTTGCAGACTGGTTCATTGCGTTGAGGTT 5 0.125 No Hit GGTATGTTGTTTTCTTGAAGGCAAATGCAGGCATCCGAGACACCGTTTGA 5 0.125 No Hit GTAAGCTTTCTTTGCCTCCTCAAGCTCAAGCAACACTTGAGATGCCTCAG 5 0.125 No Hit ATCCACCTTCTCTTGGCATTTTCTATGGCTTCCTCCCTCGTCTTCATGAG 5 0.125 No Hit GGGATCCCACGTTCACGAGCAACTTCACTGAACTGCCTCATTTTTTGCAC 5 0.125 No Hit GCCCATCTACCACTCTGTGCACTCCCTTCCTCGTATTTCTTCATCACGTC 5 0.125 No Hit TGTCACTTGCCCAGCACTGTTTGCCTCGTATGCTGCACAAGCCTCTGGGG 5 0.125 No Hit GCAGGTGCTTAATTACACTGACTGACCTAAAAATACAAACCAGACATAGA 5 0.125 No Hit TTTGATTCACAGTACGTTTGTCTAGCCTTCCAGTGACTTCCAAATGAAAG 5 0.125 No Hit ACCAGTGTTAATTACTTGTAAGCCTACCAAAATTGTCTCAACACTGTTCA 5 0.125 No Hit GTGGAGAAGACGGGCACATGAAGTAGTTCATGTCGAACATGGCTTTGGGA 5 0.125 No Hit GACCTAATGAATACAACACAAAATGGTGGATACAAGATTGAGCAGCAAGC 5 0.125 No Hit GTGTGGATGTTGTTGTTAGACACTTCTTTTTGTCTTTAAATATAAGGCGT 5 0.125 No Hit GTGGTATCCGACAAGCCCCATGCATAAACCGGGAACCTGCTCTTTGCAAC 5 0.125 No Hit GAGGATGTTATTGCCCCTCCTAAATGGATCTCTGAAAATAGCTTGTGGAT 5 0.125 No Hit CAGGAAACAAATGCAATCGGGTTTATGGAACCCCATACATTGGAAACAAG 5 0.125 No Hit ATGGAAGGATGATTTGTGATCCAAGAAAGAGGATTTGAGGTTTATAGATA 5 0.125 No Hit GCTTTGGCCTTTGGCAAGCTCGGAGTACAGTCCCGGATTCTTGTCATATT 5 0.125 No Hit GGGAGGGGAGAGAAAGAGTTGGAGTTTAGTGAAATTTCATAGTGAGATTG 5 0.125 No Hit GTGATAAGCTAAGCACCACTTTGCATGGATTGATCCTCCTCGCGAAACAA 5 0.125 No Hit CCACGGTTCCTGCCATGTTAAAAGAGAATAACGGCGGAGAAGAAGGTCTT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.0875 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.1125 0.0 0.0 0.0 0.0 80-81 0.125 0.0 0.0 0.0 0.0 82-83 0.1875 0.0 0.0 0.0 0.0 84-85 0.2375 0.0 0.0 0.0 0.0 86-87 0.4125 0.0 0.0 0.0 0.0 88-89 0.525 0.0 0.0 0.0 0.0 90-91 0.6625 0.0 0.0 0.0 0.0 92-93 0.825 0.0 0.0 0.0 0.0 94-95 0.95 0.0 0.0 0.0 0.0 96-97 1.3125 0.0 0.0 0.0 0.0 98-99 1.5499999999999998 0.0 0.0 0.0 0.0 100-101 1.7875 0.0 0.0 0.0 0.0 102-103 1.9749999999999999 0.0 0.0 0.0 0.0 104-105 2.2 0.0 0.0 0.0 0.0 106-107 2.3 0.0 0.0 0.0 0.0 108-109 2.575 0.0 0.0 0.0 0.0 110-111 2.9125 0.0 0.0 0.0 0.0 112-113 3.25 0.0 0.0 0.0 0.0 114-115 3.475 0.0 0.0 0.0 0.0 116-117 3.875 0.0 0.0 0.0 0.0 118-119 4.262499999999999 0.0 0.0 0.0 0.0 120-121 5.0 0.0 0.0 0.0 0.0 122-123 5.7125 0.0 0.0 0.0 0.0 124-125 6.4 0.0 0.0 0.0 0.0 126-127 6.9625 0.0 0.0 0.0 0.0 128-129 7.5375 0.0 0.0 0.0 0.0 130-131 7.9624999999999995 0.0 0.0 0.0 0.0 132-133 8.6375 0.0 0.0 0.0 0.0 134-135 9.225 0.0 0.0 0.0 0.0 136-137 10.1125 0.0 0.0 0.0 0.0 138-139 10.875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTAGTT 10 0.006830828 145.0 9 CAGTAGT 10 0.006830828 145.0 8 TGGAGCA 10 0.006830828 145.0 3 AGCAGTA 10 0.006830828 145.0 6 GAGCAGT 10 0.006830828 145.0 5 CTTGGAG 10 0.006830828 145.0 1 GCAGTAG 10 0.006830828 145.0 7 TTGGAGC 10 0.006830828 145.0 2 ATCGGAA 65 0.0076375785 22.307692 145 GATCGGA 65 2.0332325E-5 17.846153 140-144 >>END_MODULE SRR13695426 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695426_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.12425 37.0 37.0 37.0 37.0 37.0 2 36.3105 37.0 37.0 37.0 37.0 37.0 3 36.204 37.0 37.0 37.0 37.0 37.0 4 36.085 37.0 37.0 37.0 37.0 37.0 5 36.2955 37.0 37.0 37.0 37.0 37.0 6 36.3015 37.0 37.0 37.0 37.0 37.0 7 36.284 37.0 37.0 37.0 37.0 37.0 8 36.219 37.0 37.0 37.0 37.0 37.0 9 36.291 37.0 37.0 37.0 37.0 37.0 10-14 36.2678 37.0 37.0 37.0 37.0 37.0 15-19 36.213800000000006 37.0 37.0 37.0 37.0 37.0 20-24 36.1666 37.0 37.0 37.0 37.0 37.0 25-29 36.12140000000001 37.0 37.0 37.0 37.0 37.0 30-34 36.06205 37.0 37.0 37.0 37.0 37.0 35-39 36.0299 37.0 37.0 37.0 37.0 37.0 40-44 36.0768 37.0 37.0 37.0 37.0 37.0 45-49 36.04785 37.0 37.0 37.0 37.0 37.0 50-54 36.02975 37.0 37.0 37.0 37.0 37.0 55-59 35.987399999999994 37.0 37.0 37.0 37.0 37.0 60-64 35.9434 37.0 37.0 37.0 37.0 37.0 65-69 35.880700000000004 37.0 37.0 37.0 37.0 37.0 70-74 35.872 37.0 37.0 37.0 37.0 37.0 75-79 35.969300000000004 37.0 37.0 37.0 37.0 37.0 80-84 35.92055 37.0 37.0 37.0 37.0 37.0 85-89 35.752250000000004 37.0 37.0 37.0 37.0 37.0 90-94 35.8148 37.0 37.0 37.0 37.0 37.0 95-99 35.82425 37.0 37.0 37.0 37.0 37.0 100-104 35.792199999999994 37.0 37.0 37.0 37.0 37.0 105-109 35.777150000000006 37.0 37.0 37.0 37.0 37.0 110-114 35.6939 37.0 37.0 37.0 37.0 37.0 115-119 35.7081 37.0 37.0 37.0 37.0 37.0 120-124 35.6185 37.0 37.0 37.0 37.0 37.0 125-129 35.640550000000005 37.0 37.0 37.0 37.0 37.0 130-134 35.4921 37.0 37.0 37.0 37.0 37.0 135-139 35.4277 37.0 37.0 37.0 34.6 37.0 140-144 35.360499999999995 37.0 37.0 37.0 34.6 37.0 145-149 35.1791 37.0 37.0 37.0 29.8 37.0 150-151 34.98375 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 2.0 13 0.0 14 3.0 15 4.0 16 2.0 17 1.0 18 0.0 19 1.0 20 1.0 21 6.0 22 5.0 23 5.0 24 4.0 25 10.0 26 6.0 27 10.0 28 16.0 29 19.0 30 32.0 31 37.0 32 60.0 33 89.0 34 195.0 35 586.0 36 2685.0 37 220.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 40.8030112923463 19.648682559598495 13.274780426599749 26.27352572145546 2 29.5 23.525 28.025 18.95 3 21.6 27.875 30.275000000000002 20.25 4 25.15 34.075 23.45 17.325 5 26.924999999999997 34.4 21.425 17.25 6 20.075000000000003 39.725 20.9 19.3 7 22.1 23.075000000000003 37.225 17.599999999999998 8 21.625 26.150000000000002 28.549999999999997 23.674999999999997 9 20.95 27.025 30.049999999999997 21.975 10-14 24.240000000000002 28.95 25.645 21.165 15-19 23.68 28.470000000000002 26.755000000000003 21.095 20-24 23.897169150745224 28.808642592777833 26.748024407322195 20.54616384915475 25-29 23.36168084042021 27.723861930965484 28.274137068534266 20.640320160080037 30-34 22.797979292752462 27.42459860951333 28.179862952033215 21.597559145700995 35-39 23.016905071521457 28.13844153245974 28.008402520756228 20.83625087526258 40-44 22.31892757102841 28.256302521008404 28.04621848739496 21.37855142056823 45-49 22.382833991897165 28.610013504726655 27.654679137698196 21.352473365677987 50-54 22.8607151787947 27.991997999499873 27.311827956989248 21.835458864716177 55-59 22.156078039019512 27.63881940970485 27.908954477238616 22.296148074037017 60-64 22.176652995898767 27.28818645593678 28.78363509052716 21.751525457637293 65-69 23.83976795359072 27.325465093018604 27.760552110422083 21.074214842968594 70-74 22.96648324162081 27.63381690845423 28.16408204102051 21.235617808904454 75-79 22.919167667066827 28.626450580232092 27.37595038015206 21.07843137254902 80-84 22.870717679419855 28.37209302325581 27.936984246061513 20.820205051262818 85-89 23.220449202140962 28.467810514731628 26.987144214896702 21.324596068230704 90-94 23.70711213364009 28.32349704911473 26.753025907772333 21.216364909472844 95-99 24.01100275068767 27.446861715428856 27.41185296324081 21.13028257064266 100-104 24.282141070535268 27.24862431215608 27.068534267133565 21.400700350175086 105-109 23.410534740633285 27.2822770246611 28.367765494472515 20.939422740233105 110-114 24.037211163349003 28.26347904371311 27.683304991497447 20.01600480144043 115-119 24.199679871948778 27.095838335334133 27.73109243697479 20.9733893557423 120-124 23.66183091545773 28.74937468734367 26.848424212106053 20.740370185092548 125-129 25.23135410934921 28.172677704967235 26.44189885448452 20.15406933119904 130-134 25.077538769384695 27.458729364682345 26.60830415207604 20.855427713856926 135-139 25.032516258129068 26.993496748374184 27.55877938969485 20.415207603801903 140-144 25.787736320896272 26.763028908672602 27.338201460438132 20.111033309992997 145-149 26.078039019509752 27.423711855927962 26.488244122061033 20.01000500250125 150-151 26.338169084542272 26.850925462731368 26.775887943971988 20.035017508754375 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.0 12 0.5 13 1.0 14 1.0 15 0.0 16 0.5 17 0.5 18 1.0 19 1.5 20 1.0 21 1.0 22 0.5 23 3.0 24 3.0 25 2.0 26 5.5 27 4.0 28 4.0 29 10.5 30 9.5 31 12.0 32 26.5 33 29.5 34 33.0 35 58.0 36 86.5 37 104.0 38 130.0 39 159.0 40 213.0 41 262.5 42 228.0 43 219.0 44 289.0 45 275.5 46 234.5 47 263.0 48 260.5 49 217.5 50 171.0 51 138.0 52 121.0 53 99.0 54 74.5 55 61.0 56 42.5 57 26.5 58 25.0 59 26.0 60 20.5 61 12.5 62 8.0 63 4.5 64 2.0 65 0.5 66 0.5 67 0.5 68 1.0 69 1.5 70 0.5 71 0.0 72 0.5 73 1.0 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 1.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 1.0 98 0.5 99 0.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.375 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.03 25-29 0.05 30-34 0.034999999999999996 35-39 0.03 40-44 0.04 45-49 0.034999999999999996 50-54 0.025 55-59 0.05 60-64 0.03 65-69 0.02 70-74 0.05 75-79 0.04 80-84 0.025 85-89 0.045 90-94 0.03 95-99 0.025 100-104 0.05 105-109 0.045 110-114 0.03 115-119 0.04 120-124 0.05 125-129 0.045 130-134 0.05 135-139 0.05 140-144 0.03 145-149 0.05 150-151 0.05 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.675 #Duplication Level Percentage of deduplicated Percentage of total 1 69.62879640044994 46.425 2 18.822647169103863 25.1 3 6.786651668541433 13.575000000000001 4 2.8496437945256843 7.6 5 1.0498687664041995 3.5000000000000004 6 0.5249343832020997 2.1 7 0.18747656542932134 0.8750000000000001 8 0.11248593925759282 0.6 9 0.03749531308586427 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTTGCAACTGTTCGGTGCGAAGAGATTGCCAATGAGAAGCTTCGCTACT 9 0.22499999999999998 No Hit CCGGAATTCTTAAATAGATTGGATGAGATGATTGTTTTCCGTCAACTTAC 8 0.2 No Hit GTTCGTGCAACAAGCGAAGAGCATGGGTGAAACCATGTCGAAAACCGTCA 8 0.2 No Hit CAGAGAACACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 8 0.2 No Hit AGGCTTACTACTTGGCCGTGCCATTTCCGATTGAGAAGGCACCCACCAAG 7 0.17500000000000002 No Hit GTGGTATTATGATTCGTGAAACTGCAGAAACATTTGGGATAATCACACAA 7 0.17500000000000002 No Hit TTTCCATTTCAGGTTTGGTATTTCCTGTAATGGTGGGTTCTATTGGGATC 7 0.17500000000000002 No Hit GAACCCTTTAAAGTTGAGGAGGCTGAGACAGTAAATGTACCTCCACCCTC 7 0.17500000000000002 No Hit CGGCGATTCACCTGCTCTATGTAGTTTTGAATCTCCCAACTCATCTAGCT 7 0.17500000000000002 No Hit ATTTTGGGCTGGCTCGTGTGTCATTTACTAATGCTCCTTCTGCTATTTTT 6 0.15 No Hit CTAAAGGAGAGATTAGGTTGTGAGCCCTCAGATGAACAAATTGCAGATTC 6 0.15 No Hit GGAAATACAGGTTAGTTCTTGAAAGAAAGAGAAAGAGAAAGATCGAAATG 6 0.15 No Hit TGTGAAATGTTTGTTGGAGGATGAGGCAGTTAAAGTAGGAGGCACAAATC 6 0.15 No Hit GGCTGGGAAGGAGGTGTTGGGCACTGCTACTAAGGTGGTGATAACAAAAG 6 0.15 No Hit AGAACAACTTTCCATCTATGAGATGGATCTTGAAGAGTGGGAGCTTCTAC 6 0.15 No Hit TTTGGAAAGATGAAATGTTCCTCCAGGGCTTCATCCATTGTGTGCAAAAG 6 0.15 No Hit GGATACTCTGCCAAAGCATCAGGATGTTTCAACCTGGTTCTAATATCATT 6 0.15 No Hit AGAAGGGCTATACAGTGTTTGGTTACCTGCCAGTGGTTCCGATTGAGGAG 6 0.15 No Hit AGGTGCTCGAATCTGTTCACATTGCTGCAAACAAAAACACTGTTCCTGGT 6 0.15 No Hit CCCTGATTGTTCTTTGAAGGCTTCCCAGGTGGCTGTCAAACAATATATAA 6 0.15 No Hit GCGGCTCTGGATGCAAGTGCGGCAGTGGCTGCAATGGATGCAGCATGTAC 6 0.15 No Hit TGGCAAATCAGACGACAGACAAAAGCCCCCCAAGCTGCAGGGACTATCCA 6 0.15 No Hit GAAGTATTTGGGTGGGTCCGGAGAACCAGCCTACCCTGGCGGACCCTTGT 6 0.15 No Hit GAATACTTCATCAAGTGTACGAAATGGCTCTTCTTAACTGGAAAACCATG 5 0.125 No Hit GGGTATTCTTCTCAAGCCTAGTATGGTCACTCCTGGTGCTGAATGCAAGG 5 0.125 No Hit CTTCTGTAGCTTTCTCCAATTTCTTTTACACAGTCAAAAACCCTTTAGCA 5 0.125 No Hit GGAGTGATTGCTGGAGGTGCTGTGAGAATTGTTCTTGAAATGGCAGGTGT 5 0.125 No Hit CAGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 5 0.125 No Hit TGTTAGTGGAGGCTTACAGTGCGTAGGAAACCCAAAAGAGCTGAAGGCTA 5 0.125 No Hit AAAACATAAATTTTGTACCACTGAAACAGGAGAAAAACTTCAGAGCTGAT 5 0.125 No Hit AACACATTCGTGGCAGAAGTTCTGGGACTTCATCCTAAGGAAGTTGATGT 5 0.125 No Hit TTCAGAGACACCCTGATTGAACAAGCCGAGCAGGGTGTAGATTATTTCAC 5 0.125 No Hit CACGAGATGACTACAGAAAGCGGCAAGAGATTGATTTCTTTGGTGAGATT 5 0.125 No Hit GAGAGAGAGAGACAGAGAGAATGGCCACCACAGCAGCCCTCTCCAGCGCC 5 0.125 No Hit ATCGGCTACCAACCTTTCAGCTGATCTTTGTCCATGTGATTGAGTCACTT 5 0.125 No Hit GATTGACTGGTCATCTATCTAAGTTCTAAATTATTATTATGTAATCTTTG 5 0.125 No Hit GTCCCATTAGTCTTTTTCAATCCTATGAATGTTACTTTGCTCACCATCCA 5 0.125 No Hit GGATAAGCTGTATCAGACACGTGAACGCTCTCGTCAATTGCTATCCCATG 5 0.125 No Hit GAATGATTCAGTCAAAGAAAGCAGATTTTCCTCTTGCATAACCAAACCGG 5 0.125 No Hit CAAAACACAGAGAAGTTTCTTTGGTTTTTTTATCATGTCGTTGCTTTCAG 5 0.125 No Hit GAGGATGACACTTTGATGCCCAAGGTAAATAAAGAGATAGTGAAGGTCCA 5 0.125 No Hit TCTGCATTTGCTAGCTAGAAGTCACTCTACCCTTCGCATTACTTCCTCAA 5 0.125 No Hit CAAGGATCCAGTGCGTCCTGGTGTCAAGGATGCTGTTCAGACTTGTCTTG 5 0.125 No Hit ACTGTTGCAGCTTAGAGAGGCTGTTGTAATCTTTGATGAGATTCTAAAAA 5 0.125 No Hit CTTTACTCTCACTTCACTCTCTCAGATTTTTTTATCTCGTTCTTCTCCTT 5 0.125 No Hit CAAGGCCACGAACCGACTTCCGAAACCCTATTTCTTGGTCGGTTTCTTTT 5 0.125 No Hit GCCCTTAAGGACATTGGTCCAAAAAATTGAATATGATGCTGTAGCAGCAG 5 0.125 No Hit GTAGGAATGGGAGTATTTGCACTTGTGGTAACGGTATTTGCATTATTGAT 5 0.125 No Hit GTGGCGTTTTCCAACTGCAAATGACTTTGGATCAGAGATAATTCCAGCTT 5 0.125 No Hit GAAAAAACCTTCAAATTTCTAGTATCCCTTTTTCTTCTCCTGGTGATTCT 5 0.125 No Hit CGATTGCTCTTGTTCCCAATGTATTCGGTTCCATAAACCCGGTTGTGATT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0125 0.0 0.0 16-17 0.0 0.0 0.025 0.0 0.0 18-19 0.0 0.0 0.025 0.0 0.0 20-21 0.0 0.0 0.025 0.0 0.0 22-23 0.0 0.0 0.025 0.0 0.0 24-25 0.0 0.0 0.025 0.0 0.0 26-27 0.0 0.0 0.025 0.0 0.0 28-29 0.0 0.0 0.025 0.0 0.0 30-31 0.0 0.0 0.025 0.0 0.0 32-33 0.0 0.0 0.025 0.0 0.0 34-35 0.0 0.0 0.025 0.0 0.0 36-37 0.0 0.0 0.025 0.0 0.0 38-39 0.0 0.0 0.025 0.0 0.0 40-41 0.0 0.0 0.025 0.0 0.0 42-43 0.0 0.0 0.025 0.0 0.0 44-45 0.0 0.0 0.025 0.0 0.0 46-47 0.0 0.0 0.025 0.0 0.0 48-49 0.0 0.0 0.025 0.0 0.0 50-51 0.0 0.0 0.025 0.0 0.0 52-53 0.0 0.0 0.025 0.0 0.0 54-55 0.0 0.0 0.025 0.0 0.0 56-57 0.0 0.0 0.025 0.0 0.0 58-59 0.0 0.0 0.025 0.0 0.0 60-61 0.0 0.0 0.025 0.0 0.0 62-63 0.0 0.0 0.025 0.0 0.0 64-65 0.0 0.0 0.025 0.0 0.0 66-67 0.0 0.0 0.025 0.0 0.0 68-69 0.0 0.0 0.025 0.0 0.0 70-71 0.025 0.0 0.025 0.0 0.0 72-73 0.05 0.0 0.025 0.0 0.0 74-75 0.0875 0.0 0.025 0.0 0.0 76-77 0.1 0.0 0.025 0.0 0.0 78-79 0.1125 0.0 0.025 0.0 0.0 80-81 0.125 0.0 0.025 0.0 0.0 82-83 0.1875 0.0 0.025 0.0 0.0 84-85 0.2375 0.0 0.025 0.0 0.0 86-87 0.4125 0.0 0.025 0.0 0.0 88-89 0.525 0.0 0.025 0.0 0.0 90-91 0.6625 0.0 0.025 0.0 0.0 92-93 0.825 0.0 0.025 0.0 0.0 94-95 0.95 0.0 0.025 0.0 0.0 96-97 1.3125 0.0 0.025 0.0 0.0 98-99 1.5499999999999998 0.0 0.025 0.0 0.0 100-101 1.7875 0.0 0.025 0.0 0.0 102-103 1.9749999999999999 0.0 0.025 0.0 0.0 104-105 2.2 0.0 0.025 0.0 0.0 106-107 2.3 0.0 0.025 0.0 0.0 108-109 2.575 0.0 0.025 0.0 0.0 110-111 2.9125 0.0 0.025 0.0 0.0 112-113 3.25 0.0 0.025 0.0 0.0 114-115 3.475 0.0 0.025 0.0 0.0 116-117 3.9125 0.0 0.025 0.0 0.0 118-119 4.3125 0.0 0.025 0.0 0.0 120-121 5.050000000000001 0.0 0.025 0.0 0.0 122-123 5.7625 0.0 0.025 0.0 0.0 124-125 6.45 0.0 0.025 0.0 0.0 126-127 7.0125 0.0 0.025 0.0 0.0 128-129 7.612500000000001 0.0 0.025 0.0 0.0 130-131 8.0375 0.0 0.025 0.0 0.0 132-133 8.6875 0.0 0.025 0.0 0.0 134-135 9.2625 0.0 0.025 0.0 0.0 136-137 10.1375 0.0 0.025 0.0 0.0 138-139 10.9 0.0 0.025 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCCATC 10 0.006830828 145.0 4 ACACTGT 10 0.006830828 145.0 8 GATCTGG 10 0.006830828 145.0 5 AAGGCCT 10 0.006830828 145.0 5 TCCATCT 10 0.006830828 145.0 5 TTAATCC 10 0.006830828 145.0 1 GATCGGA 70 3.8434082E-5 16.571428 140-144 >>END_MODULE Read 1156699 spots for SRR13695426.sra Written 1156699 spots for SRR13695426.sra Read 1156699 spots for SRR13695426.sra Written 1156699 spots for SRR13695426.sra Read 1156699 spots for SRR13695426.sra Written 1156699 spots for SRR13695426.sra Read 1156699 spots for SRR13695426.sra Written 1156699 spots for SRR13695426.sra Read 1156699 spots for SRR13695426.sra Written 1156699 spots for SRR13695426.sra Read 1156699 spots for SRR13695426.sra Written 1156699 spots for SRR13695426.sra Read 1156699 spots for SRR13695426.sra Written 1156699 spots for SRR13695426.sra Read 1156699 spots for SRR13695426.sra Written 1156699 spots for SRR13695426.sra Read 1156704 spots for SRR13695426.sra Written 1156704 spots for SRR13695426.sra Read 1156699 spots for SRR13695426.sra Written 1156699 spots for SRR13695426.sra Read 1156699 spots for SRR13695426.sra Written 1156699 spots for SRR13695426.sra Read 1156699 spots for SRR13695426.sra Written 1156699 spots for SRR13695426.sra Read 1156699 spots for SRR13695426.sra Written 1156699 spots for SRR13695426.sra Read 1156699 spots for SRR13695426.sra Written 1156699 spots for SRR13695426.sra Read 1156699 spots for SRR13695426.sra Written 1156699 spots for SRR13695426.sra Read 1156699 spots for SRR13695426.sra Written 1156699 spots for SRR13695426.sra Read 1156699 spots for SRR13695426.sra Written 1156699 spots for SRR13695426.sra Read 1156699 spots for SRR13695426.sra Written 1156699 spots for SRR13695426.sra Read 1156699 spots for SRR13695426.sra Written 1156699 spots for SRR13695426.sra Read 1156699 spots for SRR13695426.sra Written 1156699 spots for SRR13695426.sra SRR ids: ['SRR13695426.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_vjd6kzpq SRR13695426.sra spots: 23133985 blocks: [[1, 1156699], [1156700, 2313398], [2313399, 3470097], [3470098, 4626796], [4626797, 5783495], [5783496, 6940194], [6940195, 8096893], [8096894, 9253592], [9253593, 10410291], [10410292, 11566990], [11566991, 12723689], [12723690, 13880388], [13880389, 15037087], [15037088, 16193786], [16193787, 17350485], [17350486, 18507184], [18507185, 19663883], [19663884, 20820582], [20820583, 21977281], [21977282, 23133985]] SRR13695426 file size 7840239 SRR13695426 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695426 SRR13695426_1.fastq SRR13695426_2.fastq Input file: SRR13695426_1.fastq Paired file: SRR13695426_2.fastq trimmed: SRR13695426-trimmed-pair1.fastq, SRR13695426-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 01:44:45 2025 >> started Wed Feb 12 01:45:12 2025 >> done (27.181s) 23133985 read pairs processed; of these: 155 ( 0.00%) short read pairs filtered out after trimming by size control 14896 ( 0.06%) empty read pairs filtered out after trimming by size control 23118934 (99.93%) read pairs available; of these: 3512209 (15.19%) trimmed read pairs available after processing 19606725 (84.81%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 7 0.00% 20 2 0.00% 21 7 0.00% 22 2 0.00% 23 0 0.00% 24 1 0.00% 25 4 0.00% 26 3 0.00% 27 3 0.00% 28 6 0.00% 29 5 0.00% 30 5 0.00% 31 7 0.00% 32 24 0.00% 33 9 0.00% 34 7 0.00% 35 19 0.00% 36 5 0.00% 37 13 0.00% 38 16 0.00% 39 32 0.00% 40 31 0.00% 41 22 0.00% 42 27 0.00% 43 35 0.00% 44 31 0.00% 45 47 0.00% 46 37 0.00% 47 55 0.00% 48 49 0.00% 49 126 0.00% 50 87 0.00% 51 147 0.00% 52 120 0.00% 53 170 0.00% 54 180 0.00% 55 235 0.00% 56 209 0.00% 57 272 0.00% 58 287 0.00% 59 375 0.00% 60 415 0.00% 61 549 0.00% 62 629 0.00% 63 679 0.00% 64 784 0.00% 65 854 0.00% 66 1050 0.00% 67 1082 0.00% 68 1303 0.01% 69 1463 0.01% 70 1753 0.01% 71 2088 0.01% 72 2310 0.01% 73 2628 0.01% 74 3044 0.01% 75 3497 0.02% 76 3724 0.02% 77 4320 0.02% 78 4675 0.02% 79 5174 0.02% 80 6029 0.03% 81 6690 0.03% 82 7562 0.03% 83 8271 0.04% 84 9812 0.04% 85 10578 0.05% 86 11137 0.05% 87 11925 0.05% 88 13436 0.06% 89 13629 0.06% 90 15207 0.07% 91 16222 0.07% 92 17682 0.08% 93 19059 0.08% 94 20829 0.09% 95 22181 0.10% 96 23254 0.10% 97 24422 0.11% 98 25345 0.11% 99 26881 0.12% 100 28385 0.12% 101 28704 0.12% 102 31300 0.14% 103 32178 0.14% 104 33923 0.15% 105 36424 0.16% 106 37294 0.16% 107 38342 0.17% 108 39626 0.17% 109 41387 0.18% 110 42183 0.18% 111 43069 0.19% 112 44659 0.19% 113 46336 0.20% 114 47592 0.21% 115 50342 0.22% 116 51723 0.22% 117 53312 0.23% 118 54859 0.24% 119 55214 0.24% 120 57090 0.25% 121 57707 0.25% 122 58635 0.25% 123 60083 0.26% 124 62407 0.27% 125 63048 0.27% 126 65378 0.28% 127 66569 0.29% 128 68479 0.30% 129 69133 0.30% 130 70677 0.31% 131 70369 0.30% 132 71810 0.31% 133 73474 0.32% 134 73029 0.32% 135 75042 0.32% 136 75781 0.33% 137 77920 0.34% 138 79254 0.34% 139 80545 0.35% 140 81238 0.35% 141 82292 0.36% 142 82959 0.36% 143 83257 0.36% 144 85093 0.37% 145 85781 0.37% 146 86782 0.38% 147 87874 0.38% 148 88812 0.38% 149 89616 0.39% 150 90332 0.39% 151 19606725 84.81% 23118934 reads passed initial QC criterion=sequence-density sequence-density=0.52 sequence-density-rank=1 fanout-score=1.98 fanout-score-rank=28 prefix-density=0.52 prefix-fanout=2.0 sequence=GTGTTGTCGAATCC criterion=fanout-score sequence-density=0.02 sequence-density-rank=28 fanout-score=186.43 fanout-score-rank=1 prefix-density=0.28 prefix-fanout=13.2 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGGGTATGAATGTGTTCTCTGTG criterion=sequence-density sequence-density=0.54 sequence-density-rank=1 fanout-score=2.15 fanout-score-rank=23 prefix-density=0.54 prefix-fanout=2.1 sequence=TGTAAGAGATGGCTTCCTC criterion=fanout-score sequence-density=0.02 sequence-density-rank=27 fanout-score=28.57 fanout-score-rank=1 prefix-density=0.39 prefix-fanout=1.3 sequence=TTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGGTACCAAGAAAAAAACGAACCTTTGGGTTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATAAGATTCCATGCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGTCAGGGCCGCTGGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGCCTTTTGGGTCCTGTATCCCAATTCTCAGAGGTCCCGCCGTACGCTGAGGACCACCTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGAGACGAATTGCCAGA SRR13695426 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 01:45:53 Started mapping on | Feb 12 01:45:53 Finished on | Feb 12 01:49:28 Mapping speed, Million of reads per hour | 387.11 Number of input reads | 23118934 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 21250823 Uniquely mapped reads % | 91.92% Average mapped length | 292.82 Number of splices: Total | 20576831 Number of splices: Annotated (sjdb) | 20183056 Number of splices: GT/AG | 20140469 Number of splices: GC/AG | 361642 Number of splices: AT/AC | 11291 Number of splices: Non-canonical | 63429 Mismatch rate per base, % | 0.37% Deletion rate per base | 0.03% Deletion average length | 2.79 Insertion rate per base | 0.02% Insertion average length | 2.29 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 503124 % of reads mapped to multiple loci | 2.18% Number of reads mapped to too many loci | 24581 % of reads mapped to too many loci | 0.11% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 5.70% % of reads unmapped: other | 0.09% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1365264 1365264 1365264 N_multimapping 503124 503124 503124 N_noFeature 604330 20766885 875937 N_ambiguous 332388 1925 118844 UnstrandedReadsAssigned:20314105 PositiveStrandReadsAssigned:482013 NegativeStrandReadsAssigned:20256042 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695426 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695426-trimmed-pair1.fastq SRR13695426-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 23,118,934 reads, 20,365,272 reads pseudoaligned [quant] estimated average fragment length: 231.908 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,130 rounds 52401 SRR13695426.ke.tsv 34699 SRR13695426.se.tsv 87100 total ==> SRR13695426.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1787.09 514 12.8046 Potri.005G024800.1.v4.1 1035 804.092 392 21.7036 Potri.004G059700.1.v4.1 961 730.119 5 0.304879 Potri.007G009000.2.v4.1 1416 1185.09 0 0 Potri.003G141000.2.v4.1 2943 2712.09 1071 17.5807 Potri.016G087400.1.v4.1 270 90.4162 1024 504.202 Potri.015G069301.1.v4.1 564 339.083 0 0 Potri.010G195200.1.v4.1 1773 1542.09 86 2.48279 Potri.012G127500.1.v4.1 977 746.108 66 3.93816 ==> SRR13695426.se.tsv <== Potri.001G166300.v4.1 2 Potri.001G448400.v4.1 255 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 432 Potri.001G212900.v4.1 9 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 7 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1 SRR13695426 completed mapping pipeline successfully