Starting /dee2/code/volunteer_pipeline.sh SRR13695427 current disk space = 3050192629760 free memory = 1339972144 SRR13695427 SRAfilesize a587148fef2ef97c44d668288f710153 SRR13695427.sra SRR13695427.sra file validated SRR13695427 is paired end SRR13695427 is conventional basespace SRR13695427 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695427_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.556 37.0 37.0 37.0 37.0 37.0 2 36.299 37.0 37.0 37.0 37.0 37.0 3 36.55 37.0 37.0 37.0 37.0 37.0 4 36.521 37.0 37.0 37.0 37.0 37.0 5 36.5635 37.0 37.0 37.0 37.0 37.0 6 36.5565 37.0 37.0 37.0 37.0 37.0 7 36.4005 37.0 37.0 37.0 37.0 37.0 8 36.5855 37.0 37.0 37.0 37.0 37.0 9 36.531 37.0 37.0 37.0 37.0 37.0 10-14 36.5443 37.0 37.0 37.0 37.0 37.0 15-19 36.52760000000001 37.0 37.0 37.0 37.0 37.0 20-24 36.533100000000005 37.0 37.0 37.0 37.0 37.0 25-29 36.476 37.0 37.0 37.0 37.0 37.0 30-34 36.46060000000001 37.0 37.0 37.0 37.0 37.0 35-39 36.4481 37.0 37.0 37.0 37.0 37.0 40-44 36.443200000000004 37.0 37.0 37.0 37.0 37.0 45-49 36.3509 37.0 37.0 37.0 37.0 37.0 50-54 36.3648 37.0 37.0 37.0 37.0 37.0 55-59 36.3393 37.0 37.0 37.0 37.0 37.0 60-64 36.3446 37.0 37.0 37.0 37.0 37.0 65-69 36.305499999999995 37.0 37.0 37.0 37.0 37.0 70-74 36.3015 37.0 37.0 37.0 37.0 37.0 75-79 36.2845 37.0 37.0 37.0 37.0 37.0 80-84 36.213300000000004 37.0 37.0 37.0 37.0 37.0 85-89 36.2534 37.0 37.0 37.0 37.0 37.0 90-94 36.156699999999994 37.0 37.0 37.0 37.0 37.0 95-99 36.1391 37.0 37.0 37.0 37.0 37.0 100-104 36.1761 37.0 37.0 37.0 37.0 37.0 105-109 36.1342 37.0 37.0 37.0 37.0 37.0 110-114 36.109700000000004 37.0 37.0 37.0 37.0 37.0 115-119 36.096199999999996 37.0 37.0 37.0 37.0 37.0 120-124 36.0214 37.0 37.0 37.0 37.0 37.0 125-129 35.9645 37.0 37.0 37.0 37.0 37.0 130-134 35.9865 37.0 37.0 37.0 37.0 37.0 135-139 35.9372 37.0 37.0 37.0 37.0 37.0 140-144 35.873599999999996 37.0 37.0 37.0 37.0 37.0 145-149 35.6754 37.0 37.0 37.0 37.0 37.0 150-151 35.56975 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 1.0 22 0.0 23 1.0 24 3.0 25 4.0 26 4.0 27 8.0 28 9.0 29 17.0 30 26.0 31 31.0 32 58.0 33 79.0 34 131.0 35 286.0 36 2971.0 37 370.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.025000000000002 10.475 12.225 47.275 2 20.688788335847157 14.203117144293614 34.841628959276015 30.266465560583207 3 19.325 16.175 24.75 39.75 4 22.025 23.65 24.05 30.275000000000002 5 23.65 31.1 24.95 20.3 6 21.375 35.25 24.275 19.1 7 14.899999999999999 26.674999999999997 40.375 18.05 8 19.25 26.450000000000003 29.4 24.9 9 17.1 25.424999999999997 33.85 23.625 10-14 19.744999999999997 29.509999999999998 27.445000000000004 23.3 15-19 19.505 28.04 28.335 24.12 20-24 20.035 28.38 27.825 23.76 25-29 20.044999999999998 27.97 28.95 23.035 30-34 19.46 29.195 27.27 24.075 35-39 20.025000000000002 28.27 28.360000000000003 23.345 40-44 19.72 29.37 27.705000000000002 23.205000000000002 45-49 19.68 28.310000000000002 27.67 24.34 50-54 19.919999999999998 27.865000000000002 27.765 24.45 55-59 20.485 27.815 27.79 23.91 60-64 19.994999999999997 28.470000000000002 27.900000000000002 23.635 65-69 19.63 27.92 28.689999999999998 23.76 70-74 20.044999999999998 28.29 27.775 23.89 75-79 21.065 28.32 27.46 23.155 80-84 20.62 28.345 27.595 23.44 85-89 20.345 28.299999999999997 28.125 23.23 90-94 20.03 27.685 28.03 24.255 95-99 20.919999999999998 27.860000000000003 27.96 23.26 100-104 20.415 29.075 27.250000000000004 23.26 105-109 20.71 27.47 28.13 23.69 110-114 20.849999999999998 28.325 27.27 23.555 115-119 21.265 28.645 27.61 22.48 120-124 21.22 27.345000000000002 28.494999999999997 22.939999999999998 125-129 21.83 28.175 27.04 22.955000000000002 130-134 21.46 28.16 27.105 23.275000000000002 135-139 21.33 28.465 26.91 23.294999999999998 140-144 22.42 27.54 26.8 23.24 145-149 21.985 28.53 26.115 23.369999999999997 150-151 22.475 29.275000000000002 26.1125 22.1375 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.0 19 0.0 20 0.5 21 2.5 22 3.0 23 2.5 24 4.5 25 7.5 26 5.5 27 5.0 28 14.0 29 20.0 30 18.5 31 20.5 32 26.5 33 30.5 34 47.0 35 64.0 36 71.0 37 99.5 38 136.5 39 161.0 40 191.5 41 209.5 42 237.5 43 274.5 44 275.0 45 282.0 46 268.0 47 253.0 48 247.5 49 195.5 50 160.0 51 140.0 52 115.0 53 93.5 54 81.0 55 69.5 56 42.5 57 26.5 58 20.0 59 19.0 60 20.0 61 11.5 62 4.5 63 3.0 64 2.0 65 5.0 66 6.5 67 4.0 68 1.5 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.5499999999999999 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.025 #Duplication Level Percentage of deduplicated Percentage of total 1 67.85851595540177 44.125 2 19.953863898500575 25.95 3 7.074202229911572 13.8 4 2.691272587466359 7.000000000000001 5 1.2687427912341407 4.125 6 0.6920415224913495 2.7 7 0.2306805074971165 1.05 8 0.1922337562475971 1.0 9 0.0 0.0 >10 0.03844675124951942 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GATAGAACATATATATTGAGCACATCACTACATGTGCTTATGTGCTCAGA 10 0.25 No Hit GCATGGTCAAGCAGCTTACTTCTAAGGGTGAAAAATGCAAAATGTAATTC 8 0.2 No Hit GCCTGATCCCTAATTCAGGGCACATCAAATAATTTCAAGAGGACACATTA 8 0.2 No Hit ATCAAGTTTCTTGAACACCATCCTGAAATGCTCACAGCTTGTCCTCGAAA 8 0.2 No Hit CCACCATCCAACACTAGTGTAGGCAATGCTAATGAAAAAACAGAGGGATG 8 0.2 No Hit CTTTTCCTCACGAGCCAGCCACCAACTCTAATCCAGAATTTGTGATGTGG 8 0.2 No Hit GCTGATTGCAAATTAGCAATATATAGTCTGTACTCTGAATCAAACAAGAT 7 0.17500000000000002 No Hit GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGGACTTATCTCGTAT 7 0.17500000000000002 TruSeq Adapter, Index 20 (97% over 37bp) GTGTTATAAACCGCAGTCTTGCAATCAGGGAGAATGCTGACGGACCAAGG 7 0.17500000000000002 No Hit TGCCAGAGCAACTTCCGTAGTTTTCTGAACGCTCTTGTCTTTGATAAAGC 7 0.17500000000000002 No Hit CTCAACGGTTGGCCTTAGCAACTCTTTCAATCTCATCTTTCTTCTTAATG 7 0.17500000000000002 No Hit CCCCTAGTAAGCTTCACAATGGCATCTTCAGCAGCGTTCATGTCAAGAGC 7 0.17500000000000002 No Hit CGCTGATATCATATGGATAAGCGTCCTTTGGTGACTGACGTTTATATGCT 6 0.15 No Hit CTCAACTCTATCGTGCCTGCTGCAGATGAAAAACCAATTTACTGAACTCA 6 0.15 No Hit GTGCTGACCAAATCGAGGTTCTCTTGCAAAAATTACTTGGTGCTGATAAA 6 0.15 No Hit TTTCTGACCAACAAGCAAATCAGCGGAGACACCAACAGGAATGGGTCGTA 6 0.15 No Hit GCTGCGATAGCAAAGATAGGAATGATGACAGATTTGTATCCCAATGCAAG 6 0.15 No Hit ATGCTAACATGTATGGATTTGCTGGATTCCAGCTCACGCAGTTAACTGCT 6 0.15 No Hit CTTCCAAATTGTCTAAGAGAAGGCGCAAGCAAAAACGATTGATCTCAGTC 6 0.15 No Hit AGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGA 6 0.15 No Hit GGAGGTTTCTGCTGCTGCATAGGTGAGAGGGAGACAGAATGATATTAAGA 6 0.15 No Hit GTTTGTTACTTGCACACATATAAACGAAAAGATAGCTAATCACAATGGGA 6 0.15 No Hit CCACAAATTTGCTACATCCGACTTCTGAAGCTTTTTCCATAACACAGCTA 6 0.15 No Hit TGTTAGCTCTTCGGCATCAATCAATGGTGCTGGGGGTGGGTCTTGGTAAT 6 0.15 No Hit CTTTCTTTTCCTTCCCATCTTCGGTTACAATTGTTCCGGTGTAAAGCACA 6 0.15 No Hit CTGGAATCACCATGGAAGCTGTAAGTGCACCTGCTGTCAATCCAGTGACC 6 0.15 No Hit GTGGGATATCAAGCATGTGGAGGATCACAGGCTGGTCAGGACCAAGCATC 6 0.15 No Hit TGCCAACCTCTAATTTAATTCTCATGAATTCGCCATAATAAAAATATAGT 6 0.15 No Hit CTGCAATTCAGCTTCAGTGGGGTTTTGTCCCAGAGATCTCATCACTGTCC 6 0.15 No Hit CAAGGAGAAACAAGATACAACCCAGGCATTCCCTCGGCCCTCCATTCTGC 6 0.15 No Hit GACCCTTCACACGCCTTCTTACATCTGCCCTTGCTTTGCGAGATGCATAC 5 0.125 No Hit GTGGAGAGGCTGAAACCTTGGCACTGGCACTGACTTTGGCATTGGAGGCA 5 0.125 No Hit CCCCAAAGCATCGATGCATGATACTGGTGACGCAGAAATGAAGATTCGCC 5 0.125 No Hit CCCAAGCAAGAACATCAACAATGTTGAAACCAACTGGATCATTTGATTTT 5 0.125 No Hit TCCCGTTCTTATCAATACCATCTGGAATTACTCCAATTGATTGCCCTTCT 5 0.125 No Hit CTACCAAGCACCAGGATCTTCGAACCTCACCTTGAGGCGCCCCAAGAAAC 5 0.125 No Hit TGCCTCGTCCATGAATTCATTAGCTTGTTCACAGGTTCACCAGATCCCTC 5 0.125 No Hit CTCCATAGCACCAATTCCATCGAACATACAATGGTTCGTGCATAGCCCAA 5 0.125 No Hit TGTTGTTGGAACAGGTGGGCCATGAGGATGACCACTGGATAATTCAAAGG 5 0.125 No Hit CCTTAACTCTGGTGCCAATCTCAGAATTGAAGTCGTAAGCATCAAGAGCA 5 0.125 No Hit ATCTTCTGGAAGTCCAAGTCCTGAAACGTTAAATGAAGGGGTCTGGTTTG 5 0.125 No Hit ATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTA 5 0.125 No Hit GGACCTTCTGCAAGTTGTTTGAGTATTACTTCAGCACCCAGAAAGCCGCC 5 0.125 No Hit CTCGGTTTCTCTATCAAGTGCTCTTCATCAGTATACAATGCATGTTAGTG 5 0.125 No Hit CTCGCTTTCCTTTTGTCATCTTCCAGGGTATTTGATTTAGCAGCCGGTGT 5 0.125 No Hit CGCCATTGATTGGTTGAACCACTTGGTAAGTTGGCTTGTCTGCTTGAACG 5 0.125 No Hit TCTCGTTGGATTCCACTGGAAGAGATCATGTTCTGACCAGGTGAACCAGT 5 0.125 No Hit TCTAGTGGTGCTTGTTTGCAACAGTGCACTGCTTTCTGATCTCACCCTTG 5 0.125 No Hit GGTAAACTTTGATAAACCATCTCCGAATCTCCTCCTTCTTGCTTTATCGC 5 0.125 No Hit CACCCGGCCAACTTCATCTTTGGTAGCACTGATCTAATGCGAACTGCCAA 5 0.125 No Hit GCCGTCAAGCATATCAACCTTCCTAACCTTCTTCTCGCCGAGAAAGTTCC 5 0.125 No Hit CGCCACCTTTGAAGAGTATCACCCTGAGCAAAAGAATAGAGCCTCCAAAC 5 0.125 No Hit CTCGTAGCTGCTTTATCAAATGAAATTATCCTTAAATGCTTATGTTCCAC 5 0.125 No Hit CCTTGCTAAGTAAAACATGTCTAAGAGAGCCATTCACCATGAACTCTGTC 5 0.125 No Hit CACTTTATCAACCTCCTCACTTACAAACAGTGAAAAGACATCATCTGCTA 5 0.125 No Hit CAATAACCAATCTTTCTGATCCCATTGCCAATCACATTGATGACTTCATA 5 0.125 No Hit CGGGTGGCAGATTAAGGGGAAGCGTGATCAATGGTGACGAGTCCGTCTGC 5 0.125 No Hit CCTTAGTAAACGTTTTTCCCACTCCGGATGTTCCAATCAAGACTGTTGGT 5 0.125 No Hit CTCCAGTCCATCCCGGTAAATTTTTTCCATAAGTAGACAAATCAACAGAA 5 0.125 No Hit TGCGAATCATATCACCAGGAGTGGGTTCCAATTTTTTGGTAGAAAGACTT 5 0.125 No Hit CTTTTACTTCCATGTATGTCTTGCTCTGGATTTCATCATAGGTCAGCCTC 5 0.125 No Hit ATTGAATGAAGCAGCTCTTCCACCTCTCCAGTCCTTTGAAGAGGGCCCAT 5 0.125 No Hit CGCTTGAAAACTAGCTTTTGTTGGGGGAGGTGGCCATTAGCAAATGCTGT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0125 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.0625 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.1875 0.0 0.0 0.0 0.0 80-81 0.21250000000000002 0.0 0.0 0.0 0.0 82-83 0.225 0.0 0.0 0.0 0.0 84-85 0.225 0.0 0.0 0.0 0.0 86-87 0.3 0.0 0.0 0.0 0.0 88-89 0.3375 0.0 0.0 0.0 0.0 90-91 0.38749999999999996 0.0 0.0 0.0 0.0 92-93 0.4625 0.0 0.0 0.0 0.0 94-95 0.5375000000000001 0.0 0.0 0.0 0.0 96-97 0.6125 0.0 0.0 0.0 0.0 98-99 0.6625 0.0 0.0 0.0 0.0 100-101 0.7875 0.0 0.0 0.0 0.0 102-103 0.9 0.0 0.0 0.0 0.0 104-105 1.0125 0.0 0.0 0.0 0.0 106-107 1.1124999999999998 0.0 0.0 0.0 0.0 108-109 1.2 0.0 0.0 0.0 0.0 110-111 1.275 0.0 0.0 0.0 0.0 112-113 1.4875 0.0 0.0 0.0 0.0 114-115 1.675 0.0 0.0 0.0 0.0 116-117 1.95 0.0 0.0 0.0 0.0 118-119 2.3125 0.0 0.0 0.0 0.0 120-121 2.5 0.0 0.0 0.0 0.0 122-123 2.7249999999999996 0.0 0.0 0.0 0.0 124-125 3.075 0.0 0.0 0.0 0.0 126-127 3.3875 0.0 0.0 0.0 0.0 128-129 3.7 0.0 0.0 0.0 0.0 130-131 4.137499999999999 0.0 0.0 0.0 0.0 132-133 4.7875 0.0 0.0 0.0 0.0 134-135 5.425 0.0 0.0 0.0 0.0 136-137 5.8625 0.0 0.0 0.0 0.0 138-139 6.225 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAAGAAG 10 0.006830828 145.0 3 GAAGCAG 10 0.006830828 145.0 6 CAATAAA 10 0.006830828 145.0 4 AAGCAGA 10 0.006830828 145.0 7 >>END_MODULE SRR13695427 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695427_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.25 37.0 37.0 37.0 37.0 37.0 2 36.14 37.0 37.0 37.0 37.0 37.0 3 36.255 37.0 37.0 37.0 37.0 37.0 4 36.327 37.0 37.0 37.0 37.0 37.0 5 36.4425 37.0 37.0 37.0 37.0 37.0 6 36.313 37.0 37.0 37.0 37.0 37.0 7 36.291 37.0 37.0 37.0 37.0 37.0 8 36.373 37.0 37.0 37.0 37.0 37.0 9 36.2975 37.0 37.0 37.0 37.0 37.0 10-14 36.30159999999999 37.0 37.0 37.0 37.0 37.0 15-19 36.2118 37.0 37.0 37.0 37.0 37.0 20-24 36.24435 37.0 37.0 37.0 37.0 37.0 25-29 36.149150000000006 37.0 37.0 37.0 37.0 37.0 30-34 36.12645 37.0 37.0 37.0 37.0 37.0 35-39 36.10615 37.0 37.0 37.0 37.0 37.0 40-44 36.098150000000004 37.0 37.0 37.0 37.0 37.0 45-49 36.1678 37.0 37.0 37.0 37.0 37.0 50-54 36.06045 37.0 37.0 37.0 37.0 37.0 55-59 36.05135 37.0 37.0 37.0 37.0 37.0 60-64 35.984950000000005 37.0 37.0 37.0 37.0 37.0 65-69 36.0045 37.0 37.0 37.0 37.0 37.0 70-74 35.915949999999995 37.0 37.0 37.0 37.0 37.0 75-79 35.9391 37.0 37.0 37.0 37.0 37.0 80-84 35.882799999999996 37.0 37.0 37.0 37.0 37.0 85-89 35.79445 37.0 37.0 37.0 37.0 37.0 90-94 35.8241 37.0 37.0 37.0 37.0 37.0 95-99 35.82015 37.0 37.0 37.0 37.0 37.0 100-104 35.84665 37.0 37.0 37.0 37.0 37.0 105-109 35.816950000000006 37.0 37.0 37.0 37.0 37.0 110-114 35.731550000000006 37.0 37.0 37.0 37.0 37.0 115-119 35.79965 37.0 37.0 37.0 37.0 37.0 120-124 35.67195 37.0 37.0 37.0 37.0 37.0 125-129 35.7402 37.0 37.0 37.0 37.0 37.0 130-134 35.618849999999995 37.0 37.0 37.0 37.0 37.0 135-139 35.62355000000001 37.0 37.0 37.0 37.0 37.0 140-144 35.47295 37.0 37.0 37.0 34.6 37.0 145-149 35.40075 37.0 37.0 37.0 37.0 37.0 150-151 35.165499999999994 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 2.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 2.0 14 0.0 15 1.0 16 2.0 17 2.0 18 0.0 19 1.0 20 3.0 21 0.0 22 4.0 23 3.0 24 2.0 25 7.0 26 8.0 27 10.0 28 20.0 29 9.0 30 21.0 31 49.0 32 64.0 33 94.0 34 189.0 35 554.0 36 2737.0 37 216.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.367469879518072 18.34839357429719 19.854417670682732 32.429718875502004 2 26.35 24.55 30.9 18.2 3 20.849999999999998 28.15 30.325000000000003 20.674999999999997 4 24.2 32.975 24.349999999999998 18.475 5 26.575 33.225 22.7 17.5 6 21.475 37.675 23.3 17.549999999999997 7 19.725 22.5 38.45 19.325 8 20.95 26.3 27.900000000000002 24.85 9 21.975 25.35 30.575000000000003 22.1 10-14 23.189999999999998 29.854999999999997 25.650000000000002 21.305 15-19 22.595000000000002 28.689999999999998 26.86 21.855 20-24 23.222772524888686 29.040972534894195 27.179948971934564 20.556305968282558 25-29 22.366775081310983 28.281210908181137 28.46634976232174 20.88566424818614 30-34 22.814829639265522 28.11327362785811 27.237704507930154 21.834192224946214 35-39 22.357296513082193 27.935364450447747 28.460653359347642 21.246685677122418 40-44 22.764797118126783 28.873767949166957 27.417821584029618 20.94361334867664 45-49 22.578547128276966 28.231939163498097 27.70662397438463 21.482889733840306 50-54 23.09770373705538 27.94036720196108 27.550152583921157 21.411776477062382 55-59 23.49762321741306 28.941706279709784 26.95021265949462 20.610457843382537 60-64 22.572414828155484 28.005402971634396 28.00040022012107 21.42178198008905 65-69 23.016508254127064 27.71385692846423 27.548774387193596 21.720860430215108 70-74 23.592694520890667 27.52564423317488 27.310482862146614 21.57117838378784 75-79 22.785950165115583 27.999599719803864 28.004603222255582 21.209846892824977 80-84 23.5967983991996 27.388694347173587 27.443721860930463 21.570785392696347 85-89 23.102326745058793 27.175381536152116 28.10607955966975 21.61621215911934 90-94 22.84870922553532 28.126876125675405 27.731638983390035 21.292775665399237 95-99 22.657461603882133 27.52013607484116 28.350592826054328 21.47180949522237 100-104 23.267450587940957 27.000250187640727 28.501376032024016 21.230923192394297 105-109 23.867900925694272 28.656492369276958 26.915186389792346 20.560420315236428 110-114 24.29836410025514 27.725248886887787 27.47010855970784 20.506278453149232 115-119 24.29822366775081 28.51138353765324 27.090317738303725 20.10007505629222 120-124 24.978734050537906 27.775831873905428 26.845133850387793 20.400300225168877 125-129 24.47213049134394 28.06964875412789 26.24337035925148 21.214850395276695 130-134 24.218163622717036 28.261195896922693 26.900175131348515 20.62046534901176 135-139 25.143857893420062 27.390542907180386 27.435576682511886 20.030022516887666 140-144 25.12882085146831 29.181049577267498 26.369503226774725 19.32062634448947 145-149 25.489116837628224 28.111083312484364 25.879409557167875 20.52039029271954 150-151 26.507380535401552 27.145359019264447 27.057793345008758 19.289467100325243 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 1.0 8 1.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.0 19 1.0 20 1.0 21 0.0 22 0.5 23 1.0 24 3.5 25 3.0 26 3.0 27 6.0 28 9.0 29 14.5 30 16.0 31 12.5 32 20.5 33 31.5 34 46.5 35 68.0 36 80.5 37 112.5 38 153.5 39 179.0 40 211.0 41 241.0 42 268.0 43 272.5 44 256.0 45 244.0 46 239.5 47 237.0 48 218.5 49 200.0 50 173.5 51 140.0 52 120.5 53 96.0 54 78.0 55 60.0 56 43.5 57 42.0 58 27.5 59 15.0 60 10.0 61 7.0 62 4.5 63 2.5 64 2.0 65 2.5 66 2.0 67 0.0 68 0.0 69 0.5 70 1.0 71 0.5 72 0.0 73 0.0 74 0.0 75 0.5 76 0.5 77 0.0 78 0.0 79 1.0 80 1.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 1.0 95 1.0 96 0.0 97 1.0 98 2.0 99 1.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.055 25-29 0.075 30-34 0.065 35-39 0.055 40-44 0.065 45-49 0.06 50-54 0.055 55-59 0.075 60-64 0.055 65-69 0.05 70-74 0.075 75-79 0.06999999999999999 80-84 0.05 85-89 0.075 90-94 0.06 95-99 0.055 100-104 0.075 105-109 0.075 110-114 0.055 115-119 0.075 120-124 0.075 125-129 0.06999999999999999 130-134 0.075 135-139 0.075 140-144 0.055 145-149 0.075 150-151 0.075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.2 #Duplication Level Percentage of deduplicated Percentage of total 1 68.71165644171779 44.800000000000004 2 19.095092024539877 24.9 3 6.78680981595092 13.275 4 2.875766871165644 7.5 5 1.4187116564417177 4.625 6 0.651840490797546 2.55 7 0.23006134969325154 1.05 8 0.15337423312883436 0.8 9 0.0 0.0 >10 0.07668711656441718 0.5 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGTGGTCCTACTACTTTCTCAAAGTATTTCAAGGAGAGGTGCCCAGATGC 10 0.25 No Hit ATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAAT 10 0.25 No Hit GCACAAATGATCTAAGATAAGAGAAGAGGATTCCTTGGAATGATGCAGGC 8 0.2 No Hit GTGGTTCTTCATTCCGATGCCATTCACAGAGGTGGTGGGCAGGTCATTCC 8 0.2 No Hit CACCAAACGAACATGACGAGTTGGAAGTTATTGAAAGTCCTTTGATTTTT 8 0.2 No Hit CTAGGATTGGTGGAGGGAAACTTGATTTTAGTTCTTCTTGCACACAAAGA 8 0.2 No Hit TGAACCATGACACACAATCTTGTATGGACCCTAATGTGATGGAGGCCAAG 7 0.17500000000000002 No Hit CGGAACTGCCGTTGCAATTGCCGGAGTGGCAACCTACTCTTACATCAAGG 7 0.17500000000000002 No Hit TTTAGTGCAGTTTGTTGAAAGCATGTCAGAGGGAGGTCGCCACGGAAAAC 7 0.17500000000000002 No Hit TGTTGTCAGACGTCAGGCTGTTGATATTTCACCTCTTAGGCGTGTAAACC 7 0.17500000000000002 No Hit CGGGAATTTTGGTGGGAAAATGTCATTCCTGCCAGGGAAGCTTTGTTGAT 7 0.17500000000000002 No Hit TTCATTGCCACTAGCTACGATTATGATGCCCCTCTTGATGAATTCGGATT 7 0.17500000000000002 No Hit CACTTCCCTTCGCTATCAACGAACGATCTTAAACGAATCGCTCTCTCTCT 6 0.15 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 6 0.15 No Hit CAAGAGGCCAAAACCTCTCTTCTTCTGTCAACCCTTCTTTGAAAAACTTG 6 0.15 No Hit GCCAGAGCTCATAAGCCAAGATAACAATAAATCCTCAGCAACCCTATTCT 6 0.15 No Hit GTAAATTTGTCTGTTCTTAAACACGCCACAATGTCAGACGGTCATGAGAC 6 0.15 No Hit GCCGGAATGAATTTAATCAATGTTGGCCTCGGCATTGATCTGTTTAATGC 6 0.15 No Hit GGAGCGAAGATCAGAGAAACAAATGGCTTCAACTTCAGCTGTTTCAATGG 6 0.15 No Hit AATTGTTGAAGTGCAGAGTGCTACTTGTGTATTGTTCTCCCTAAAGCTGA 6 0.15 No Hit CTAATTATCACGTCATTCGTGGTGCTTCTGATCTCAAGGTCACTCTTGCT 6 0.15 No Hit GCTCAGAGTTGCATGAGATGAATATCATAATTCAAGCATTACTAAGTGCA 6 0.15 No Hit AGCAGATGGGGCATGCTAGAGTGCACCAGCAGCAGCATCAGCAACAAAAT 6 0.15 No Hit GACTTGCAAGTGCATCGTATCTCACACATTTCTGTTTCACCAGATTTGAT 6 0.15 No Hit TCAGCTTGAAAGAAGTAGAATAGCATAAAACAAAAATTAAAATTGTACAG 6 0.15 No Hit TATGGATTTCTTGAATTGTCTTGACCATGACACATCAATTAAGATTCTCA 6 0.15 No Hit GATTATAAGTATGTGTAGACCGACTGCAATTTTATTGCTTGATAGAGAAG 6 0.15 No Hit TAATGTTTCTCTTTGAACTATGGTTGTATGTTTCCTTGAGAGGATTCGTT 6 0.15 No Hit TATTATTGATGTTCTTTTGAGAAAAACGTAGCGCCATTCTAGTTGGGTCA 6 0.15 No Hit GCACAAACCAAAGAGAGGGAAAAACTAAAACAACTCTTCGAAGAAGCTTA 5 0.125 No Hit ATCGATGCCTGGTTTGCAACAAGAAAGACCAGTGCTGCTATCAGGACTGC 5 0.125 No Hit GACAGCAGTCGGAACTAAATGGCTGCCTCACTACAAGCAGCAGCTACACT 5 0.125 No Hit GTGTTCTTTAACTTCCAAAGGGAGAACGTGGCAAAACAAGTGCCTGAGCA 5 0.125 No Hit TTTTTCCACAATGTCTTGCAACCTCCCGAGGATGAGGTGGGGGGGACATA 5 0.125 No Hit CTTGGTGAGGCTATGGTTGGGCTTAATTTGAATGATAAGAAGATCGAGAG 5 0.125 No Hit CTACCAACTGCCTTGCTCCCCTTGCTAAGGTCATTCATGACAGATTTGGC 5 0.125 No Hit AGCTGAAATTCTCTCAAAGCTTCACCACCCCAATGTTGTTGCATTTTATG 5 0.125 No Hit CAGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 5 0.125 No Hit GGCACCGGCACCGGCTCTACAGCACAGGGGTCAGAGTCAGACTCGGTGAA 5 0.125 No Hit TTCTGGTTTTGTTGCATGAATCTTAATACAGCTTATGATACACTAACTTC 5 0.125 No Hit GGGGTTTTAATAATTATTTAATCAAGAAAGCAGAAGCAAGAATTGCAGAC 5 0.125 No Hit CCCACCTTCTTCTTCTGCCCCCCCGAACGCCAGTTCCTCTCTCTCTCTCT 5 0.125 No Hit ATCATCACAACCATGGCAGCTGCAGTAACTGCTGCAGTCTCCTTTCCTTC 5 0.125 No Hit AAGGTGACTGATTCACAAGGTCGCACTGTCAGTTTCACGAACACTGTCAT 5 0.125 No Hit CAAAAACAAAAAACCTATAGAACTTGCCTTCACTCGAACTCCAGAATGAG 5 0.125 No Hit ATTACAGACATCAATGGAGCGCGCTGCTTTATTACTAGGACAGGGTACAC 5 0.125 No Hit GCACACAACACAAACAAGACCAGCAAAAACCAGGACAAAAAAGTTCAAGA 5 0.125 No Hit TGGATACCATGTTGGCAAGGTTTATGTCATCATGTAATGGTTCTGGTGAT 5 0.125 No Hit GTGGCCATATCAGCCTACTTGGTCTTGACAGCTCTCTACAGCATATGGGA 5 0.125 No Hit CTCTATACTCCAAGCTGTTTTAACAAAAGAAAAAAGAAGGCAGTCAAATG 5 0.125 No Hit CATGGACATCCCTCCGATGGGTGGCATCCCATGCAAACGGGCATGGACCA 5 0.125 No Hit GTTACTTGCTCGTGCAATCAATGCTTTTTTAGATCAAGGAGGCAACATTT 5 0.125 No Hit TACTCCAAATGTTCCTGATATAATGGTTGCTCCACCTGAGGATGATCACC 5 0.125 No Hit TTTTTTTTACCCTTTTAGATAATTAATCTATGTTTTTTTCCTTAACAGGA 5 0.125 No Hit TCTGTTTGTAGTAATAACATGACGGAGCAGCTAACAGAGGAACAGATCGC 5 0.125 No Hit ACAAACATGAGTTTCATTCAGAAAAGGAAGCAGCCTGCTGGTGAGCCACA 5 0.125 No Hit CAAGATAAGAATTCGACCTCCTAATGCTAAAGGGAGATTGGAAATTTTGA 5 0.125 No Hit AACAAGCAGAGGGTTGCTACTGTTGTGGCACACGAGCTAGCACACCAGTG 5 0.125 No Hit GCTTATGGTGCTGTCAAGAAGTCTGTTCAGGCTTATGTCACGCGCGCTGG 5 0.125 No Hit TTAGCCATGATCGTGTTTCTGATATTGCAACAGTTCTTCAGCCTGGTGAT 5 0.125 No Hit GCTAAGCACACAAATTAAGGCTTAAAGATATAGAGAGAAAGAAACAACAT 5 0.125 No Hit GAAGCTGGAACTGGTATAGCAGAGCTTATAGCTCTTGAGATGTCAAGACG 5 0.125 No Hit CTTTCATTAAAAACTGGATTGAACTTGGGCTCACTGTGTGTCCCAAGACA 5 0.125 No Hit CCCAGACCCTAAAGCTGTCCAATATGTGAGGAATGACAGAGGCACACCCA 5 0.125 No Hit CTTGAGGACAGAAAGTATTCTACTCTGGCTCCATTTGGTGGCTTTGCATT 5 0.125 No Hit GTGCTGTTTTTGTTGAGGCTGAAACGGATTGTGAAATAGCCGAGCTTGGA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0125 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.037500000000000006 0.0 0.0 0.0 0.0 76-77 0.07500000000000001 0.0 0.0 0.0 0.0 78-79 0.16249999999999998 0.0 0.0 0.0 0.0 80-81 0.1875 0.0 0.0 0.0 0.0 82-83 0.2 0.0 0.0 0.0 0.0 84-85 0.2 0.0 0.0 0.0 0.0 86-87 0.275 0.0 0.0 0.0 0.0 88-89 0.3125 0.0 0.0 0.0 0.0 90-91 0.36250000000000004 0.0 0.0 0.0 0.0 92-93 0.4375 0.0 0.0 0.0 0.0 94-95 0.5125 0.0 0.0 0.0 0.0 96-97 0.5874999999999999 0.0 0.0 0.0 0.0 98-99 0.6375 0.0 0.0 0.0 0.0 100-101 0.7749999999999999 0.0 0.0 0.0 0.0 102-103 0.9 0.0 0.0 0.0 0.0 104-105 1.0125 0.0 0.0 0.0 0.0 106-107 1.1124999999999998 0.0 0.0 0.0 0.0 108-109 1.2 0.0 0.0 0.0 0.0 110-111 1.275 0.0 0.0 0.0 0.0 112-113 1.4875 0.0 0.0 0.0 0.0 114-115 1.675 0.0 0.0 0.0 0.0 116-117 1.95 0.0 0.0 0.0 0.0 118-119 2.3125 0.0 0.0 0.0 0.0 120-121 2.5 0.0 0.0 0.0 0.0 122-123 2.7249999999999996 0.0 0.0 0.0 0.0 124-125 3.075 0.0 0.0 0.0 0.0 126-127 3.375 0.0 0.0 0.0 0.0 128-129 3.675 0.0 0.0 0.0 0.0 130-131 4.112500000000001 0.0 0.0 0.0 0.0 132-133 4.7625 0.0 0.0 0.0 0.0 134-135 5.4125 0.0 0.0 0.0 0.0 136-137 5.9 0.0 0.0 0.0 0.0 138-139 6.2875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCATAC 10 0.006830828 145.0 2 CAACATC 10 0.006830828 145.0 4 CATACTC 10 0.006830828 145.0 4 ATTCATA 10 0.006830828 145.0 1 TCATACT 10 0.006830828 145.0 3 >>END_MODULE Read 1180593 spots for SRR13695427.sra Written 1180593 spots for SRR13695427.sra Read 1180593 spots for SRR13695427.sra Written 1180593 spots for SRR13695427.sra Read 1180593 spots for SRR13695427.sra Written 1180593 spots for SRR13695427.sra Read 1180593 spots for SRR13695427.sra Written 1180593 spots for SRR13695427.sra Read 1180593 spots for SRR13695427.sra Written 1180593 spots for SRR13695427.sra Read 1180593 spots for SRR13695427.sra Written 1180593 spots for SRR13695427.sra Read 1180593 spots for SRR13695427.sra Written 1180593 spots for SRR13695427.sra Read 1180593 spots for SRR13695427.sra Written 1180593 spots for SRR13695427.sra Read 1180593 spots for SRR13695427.sra Written 1180593 spots for SRR13695427.sra Read 1180593 spots for SRR13695427.sra Written 1180593 spots for SRR13695427.sra Read 1180593 spots for SRR13695427.sra Written 1180593 spots for SRR13695427.sra Read 1180593 spots for SRR13695427.sra Written 1180593 spots for SRR13695427.sra Read 1180593 spots for SRR13695427.sra Written 1180593 spots for SRR13695427.sra Read 1180593 spots for SRR13695427.sra Written 1180593 spots for SRR13695427.sra Read 1180593 spots for SRR13695427.sra Written 1180593 spots for SRR13695427.sra Read 1180593 spots for SRR13695427.sra Written 1180593 spots for SRR13695427.sra Read 1180593 spots for SRR13695427.sra Written 1180593 spots for SRR13695427.sra Read 1180593 spots for SRR13695427.sra Written 1180593 spots for SRR13695427.sra Read 1180593 spots for SRR13695427.sra Written 1180593 spots for SRR13695427.sra Read 1180598 spots for SRR13695427.sra Written 1180598 spots for SRR13695427.sra SRR ids: ['SRR13695427.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_mgk4ocvn SRR13695427.sra spots: 23611865 blocks: [[1, 1180593], [1180594, 2361186], [2361187, 3541779], [3541780, 4722372], [4722373, 5902965], [5902966, 7083558], [7083559, 8264151], [8264152, 9444744], [9444745, 10625337], [10625338, 11805930], [11805931, 12986523], [12986524, 14167116], [14167117, 15347709], [15347710, 16528302], [16528303, 17708895], [17708896, 18889488], [18889489, 20070081], [20070082, 21250674], [21250675, 22431267], [22431268, 23611865]] SRR13695427 file size 8002644 SRR13695427 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695427 SRR13695427_1.fastq SRR13695427_2.fastq Input file: SRR13695427_1.fastq Paired file: SRR13695427_2.fastq trimmed: SRR13695427-trimmed-pair1.fastq, SRR13695427-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 02:14:44 2025 >> started Wed Feb 12 02:15:13 2025 >> done (29.261s) 23611865 read pairs processed; of these: 137 ( 0.00%) short read pairs filtered out after trimming by size control 38762 ( 0.16%) empty read pairs filtered out after trimming by size control 23572966 (99.84%) read pairs available; of these: 2256299 ( 9.57%) trimmed read pairs available after processing 21316667 (90.43%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 4 0.00% 20 2 0.00% 21 0 0.00% 22 1 0.00% 23 1 0.00% 24 4 0.00% 25 4 0.00% 26 0 0.00% 27 5 0.00% 28 8 0.00% 29 0 0.00% 30 4 0.00% 31 3 0.00% 32 5 0.00% 33 2 0.00% 34 9 0.00% 35 12 0.00% 36 8 0.00% 37 13 0.00% 38 6 0.00% 39 17 0.00% 40 20 0.00% 41 23 0.00% 42 52 0.00% 43 32 0.00% 44 35 0.00% 45 30 0.00% 46 39 0.00% 47 64 0.00% 48 68 0.00% 49 82 0.00% 50 76 0.00% 51 132 0.00% 52 138 0.00% 53 128 0.00% 54 160 0.00% 55 131 0.00% 56 169 0.00% 57 207 0.00% 58 246 0.00% 59 330 0.00% 60 330 0.00% 61 422 0.00% 62 427 0.00% 63 530 0.00% 64 483 0.00% 65 681 0.00% 66 719 0.00% 67 847 0.00% 68 901 0.00% 69 940 0.00% 70 1193 0.01% 71 1264 0.01% 72 1482 0.01% 73 1802 0.01% 74 1997 0.01% 75 2248 0.01% 76 2528 0.01% 77 2725 0.01% 78 2906 0.01% 79 3146 0.01% 80 3754 0.02% 81 4147 0.02% 82 4519 0.02% 83 5144 0.02% 84 5762 0.02% 85 6415 0.03% 86 6829 0.03% 87 7137 0.03% 88 7519 0.03% 89 7961 0.03% 90 8773 0.04% 91 9423 0.04% 92 10008 0.04% 93 10747 0.05% 94 12332 0.05% 95 12734 0.05% 96 13468 0.06% 97 14252 0.06% 98 14418 0.06% 99 15276 0.06% 100 15916 0.07% 101 16600 0.07% 102 17790 0.08% 103 18564 0.08% 104 19041 0.08% 105 20369 0.09% 106 22026 0.09% 107 22321 0.09% 108 23051 0.10% 109 24277 0.10% 110 24447 0.10% 111 25000 0.11% 112 26431 0.11% 113 26828 0.11% 114 28202 0.12% 115 29604 0.13% 116 30808 0.13% 117 31607 0.13% 118 32890 0.14% 119 33531 0.14% 120 34182 0.15% 121 35449 0.15% 122 36197 0.15% 123 37051 0.16% 124 38389 0.16% 125 39213 0.17% 126 41235 0.17% 127 42768 0.18% 128 42941 0.18% 129 43722 0.19% 130 45134 0.19% 131 45578 0.19% 132 45823 0.19% 133 47518 0.20% 134 48186 0.20% 135 49531 0.21% 136 50756 0.22% 137 52601 0.22% 138 53733 0.23% 139 55249 0.23% 140 55951 0.24% 141 56300 0.24% 142 57714 0.24% 143 58104 0.25% 144 60681 0.26% 145 60175 0.26% 146 61942 0.26% 147 63407 0.27% 148 64472 0.27% 149 65656 0.28% 150 66879 0.28% 151 21316667 90.43% 23572966 reads passed initial QC criterion=sequence-density sequence-density=0.43 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=21 prefix-density=0.43 prefix-fanout=2.0 sequence=GTGTTGTCGAATCC criterion=fanout-score sequence-density=0.01 sequence-density-rank=25 fanout-score=408.89 fanout-score-rank=1 prefix-density=0.25 prefix-fanout=16.3 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGAGTATGAATGTGTTCTCTGTG criterion=sequence-density sequence-density=0.36 sequence-density-rank=1 fanout-score=2.19 fanout-score-rank=23 prefix-density=0.37 prefix-fanout=2.1 sequence=TACCTTCTTCGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=31 fanout-score=51.41 fanout-score-rank=1 prefix-density=0.09 prefix-fanout=5.5 sequence=AAACAAGAGAGGTGGAGATATAGGAGAGCATAACCATGTTAGTCCCATATATTTCCAAGATGAAGGCCTTTCTTATCGCATGCATTCTCTTAGCTACCATCGTCTTCTCTCCCCTGTCCACTTGCACTGCTCGAGAATTGGCCGAGCGAGACGTATCCCGGGGAGCTCTCAACCCCCATAAACCAGTGT SRR13695427 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 02:15:50 Started mapping on | Feb 12 02:15:51 Finished on | Feb 12 02:18:41 Mapping speed, Million of reads per hour | 499.19 Number of input reads | 23572966 Average input read length | 297 UNIQUE READS: Uniquely mapped reads number | 22196879 Uniquely mapped reads % | 94.16% Average mapped length | 296.01 Number of splices: Total | 21968626 Number of splices: Annotated (sjdb) | 21497218 Number of splices: GT/AG | 21518512 Number of splices: GC/AG | 363097 Number of splices: AT/AC | 12116 Number of splices: Non-canonical | 74901 Mismatch rate per base, % | 0.37% Deletion rate per base | 0.03% Deletion average length | 2.94 Insertion rate per base | 0.02% Insertion average length | 2.43 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 659555 % of reads mapped to multiple loci | 2.80% Number of reads mapped to too many loci | 34248 % of reads mapped to too many loci | 0.15% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.81% % of reads unmapped: other | 0.09% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 716805 716805 716805 N_multimapping 659555 659555 659555 N_noFeature 756106 21696431 1038303 N_ambiguous 367069 2051 147282 UnstrandedReadsAssigned:21073704 PositiveStrandReadsAssigned:498397 NegativeStrandReadsAssigned:21011294 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695427 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695427-trimmed-pair1.fastq SRR13695427-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 23,572,966 reads, 21,047,748 reads pseudoaligned [quant] estimated average fragment length: 253.462 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 971 rounds 52401 SRR13695427.ke.tsv 34699 SRR13695427.se.tsv 87100 total ==> SRR13695427.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1765.54 823.577 19.4981 Potri.005G024800.1.v4.1 1035 782.538 306 16.3448 Potri.004G059700.1.v4.1 961 708.647 27 1.59257 Potri.007G009000.2.v4.1 1416 1163.54 0 0 Potri.003G141000.2.v4.1 2943 2690.54 797 12.3818 Potri.016G087400.1.v4.1 270 81.2844 981 504.459 Potri.015G069301.1.v4.1 564 320.371 0 0 Potri.010G195200.1.v4.1 1773 1520.54 81 2.22665 Potri.012G127500.1.v4.1 977 724.62 183 10.5561 ==> SRR13695427.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 531 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 327 Potri.001G212900.v4.1 897 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 16 Potri.001G416900.v4.1 1 Potri.001G452600.v4.1 2 SRR13695427 completed mapping pipeline successfully