Starting /dee2/code/volunteer_pipeline.sh SRR13695428 current disk space = 3050801524736 free memory = 1058221608 SRR13695428 SRAfilesize 8b9e643ca16081ca49bfdfbf78744cee SRR13695428.sra SRR13695428.sra file validated SRR13695428 is paired end SRR13695428 is conventional basespace SRR13695428 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695428_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.55 37.0 37.0 37.0 37.0 37.0 2 36.29075 37.0 37.0 37.0 37.0 37.0 3 36.601 37.0 37.0 37.0 37.0 37.0 4 36.605 37.0 37.0 37.0 37.0 37.0 5 36.509 37.0 37.0 37.0 37.0 37.0 6 36.541 37.0 37.0 37.0 37.0 37.0 7 36.428 37.0 37.0 37.0 37.0 37.0 8 36.588 37.0 37.0 37.0 37.0 37.0 9 36.519 37.0 37.0 37.0 37.0 37.0 10-14 36.505 37.0 37.0 37.0 37.0 37.0 15-19 36.5278 37.0 37.0 37.0 37.0 37.0 20-24 36.5301 37.0 37.0 37.0 37.0 37.0 25-29 36.4398 37.0 37.0 37.0 37.0 37.0 30-34 36.416 37.0 37.0 37.0 37.0 37.0 35-39 36.386900000000004 37.0 37.0 37.0 37.0 37.0 40-44 36.4176 37.0 37.0 37.0 37.0 37.0 45-49 36.328700000000005 37.0 37.0 37.0 37.0 37.0 50-54 36.3292 37.0 37.0 37.0 37.0 37.0 55-59 36.3252 37.0 37.0 37.0 37.0 37.0 60-64 36.29549999999999 37.0 37.0 37.0 37.0 37.0 65-69 36.2579 37.0 37.0 37.0 37.0 37.0 70-74 36.2567 37.0 37.0 37.0 37.0 37.0 75-79 36.20219999999999 37.0 37.0 37.0 37.0 37.0 80-84 36.140499999999996 37.0 37.0 37.0 37.0 37.0 85-89 36.1677 37.0 37.0 37.0 37.0 37.0 90-94 36.0499 37.0 37.0 37.0 37.0 37.0 95-99 36.1094 37.0 37.0 37.0 37.0 37.0 100-104 36.0805 37.0 37.0 37.0 37.0 37.0 105-109 36.0638 37.0 37.0 37.0 37.0 37.0 110-114 35.997 37.0 37.0 37.0 37.0 37.0 115-119 35.915 37.0 37.0 37.0 37.0 37.0 120-124 35.849000000000004 37.0 37.0 37.0 37.0 37.0 125-129 35.8617 37.0 37.0 37.0 37.0 37.0 130-134 35.920300000000005 37.0 37.0 37.0 37.0 37.0 135-139 35.817 37.0 37.0 37.0 37.0 37.0 140-144 35.67999999999999 37.0 37.0 37.0 37.0 37.0 145-149 35.5812 37.0 37.0 37.0 37.0 37.0 150-151 35.311 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 1.0 22 0.0 23 0.0 24 3.0 25 3.0 26 5.0 27 10.0 28 16.0 29 23.0 30 25.0 31 31.0 32 63.0 33 81.0 34 138.0 35 349.0 36 2935.0 37 316.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.5 10.174999999999999 8.475000000000001 50.849999999999994 2 16.536818296054285 13.2696657451621 43.30233727067102 26.891178688112593 3 18.55 15.625 25.75 40.075 4 21.65 25.15 22.55 30.65 5 24.175 30.85 25.25 19.725 6 21.475 34.225 23.575 20.724999999999998 7 14.374999999999998 29.875 39.7 16.05 8 18.35 26.875 32.275 22.5 9 17.5 22.625 34.599999999999994 25.275 10-14 19.985 30.209999999999997 27.505000000000003 22.3 15-19 19.475 28.485 28.139999999999997 23.9 20-24 19.62 28.315 29.020000000000003 23.044999999999998 25-29 19.869999999999997 28.599999999999998 28.275 23.255 30-34 19.465 28.09 28.310000000000002 24.135 35-39 20.105 29.299999999999997 27.145000000000003 23.45 40-44 20.315 28.33 28.09 23.265 45-49 19.775000000000002 29.175 26.939999999999998 24.11 50-54 19.5 28.560000000000002 28.02 23.919999999999998 55-59 19.61 28.12 28.775000000000002 23.494999999999997 60-64 20.365 27.994999999999997 28.43 23.21 65-69 20.3 28.935 27.115000000000002 23.65 70-74 20.325 27.565 28.835 23.275000000000002 75-79 20.465 29.53 27.21 22.795 80-84 20.43 27.975 28.084999999999997 23.51 85-89 19.885 29.28 27.325 23.51 90-94 19.715 28.485 28.065 23.735 95-99 20.45 29.299999999999997 27.47 22.78 100-104 21.0 28.265 28.07 22.665 105-109 20.605 28.244999999999997 27.49 23.66 110-114 20.915 27.810000000000002 28.310000000000002 22.965 115-119 20.765 28.92 27.315 23.0 120-124 20.925 28.23 27.644999999999996 23.200000000000003 125-129 20.845 28.425 26.99 23.74 130-134 21.54 28.735 26.71 23.015 135-139 20.735 28.895 26.669999999999998 23.7 140-144 20.979999999999997 28.95 27.034999999999997 23.035 145-149 21.154999999999998 28.349999999999998 27.38 23.115 150-151 20.9375 27.800000000000004 26.724999999999998 24.5375 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.5 20 1.0 21 0.0 22 0.5 23 3.0 24 2.5 25 1.0 26 1.5 27 5.0 28 10.0 29 17.5 30 24.0 31 26.0 32 37.0 33 43.0 34 48.0 35 69.0 36 104.5 37 142.0 38 147.0 39 172.0 40 196.0 41 220.5 42 250.5 43 250.0 44 272.0 45 272.5 46 230.5 47 225.5 48 234.5 49 190.0 50 163.5 51 153.5 52 108.5 53 70.5 54 66.0 55 71.5 56 52.5 57 37.0 58 31.5 59 16.0 60 12.0 61 9.5 62 4.0 63 1.5 64 0.5 65 1.0 66 1.5 67 1.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.525 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.95 #Duplication Level Percentage of deduplicated Percentage of total 1 70.23546725533481 47.725 2 19.426048565121413 26.400000000000002 3 6.033848417954378 12.3 4 2.4282560706401766 6.6000000000000005 5 1.287711552612215 4.375 6 0.40470934510669615 1.6500000000000001 7 0.14716703458425312 0.7000000000000001 8 0.0 0.0 9 0.0 0.0 >10 0.03679175864606328 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGCGACAGTAGAGGATGGTCTGGTGGGCTGGGCTTCAATAACAGGTGGAG 10 0.25 No Hit CCAGCCTATACTCTTTATACCCTTTATCAGACAAAACTTTACCAGCACCT 7 0.17500000000000002 No Hit GGAGGAACATCTAAAGCAGTAAATCCACTGATGCATTGAGCTGAAACTCC 7 0.17500000000000002 No Hit TGCCACTAGATGTTTTATAACAAGAAGATAAGCATACATCTGTGTGAGCA 7 0.17500000000000002 No Hit CCTTGTTCCACATAAAGTAAAATGATAAATAAGAGAAGGCCTCTAATCCT 7 0.17500000000000002 No Hit ATCCAAGTCCGTCGACGTCGGGAACTAGCTCAAACATGCTCGAGCACCCC 6 0.15 No Hit CAATCATCTACTCAATCCTGACAAGGAAAGAACACTGCAAGCATCATGTT 6 0.15 No Hit CTACTTTTTATTCAACAATTCTCGCCCGATTCAGCATCCGAATCCAGAAG 6 0.15 No Hit TGTTGCAGTTGCTCCCTCGAATCTCCATCTTCTTCATCTATAGATTTCAA 6 0.15 No Hit CATGGCTGGAACTGCTGGTGAACGGTAATCCTCGTTGTTCCTATTCATTG 6 0.15 No Hit GAGTGACCTGGAGTATCAATTATATTGATTTTTGTATCCTTATAAGTAAT 6 0.15 No Hit CACAACTCCAAATGAGTACACGTCGGATTTTTCAGTTAGTTGTTGCCTCC 6 0.15 No Hit CTGATTTGTTTCGTTTTCCCAGCTTCTTCTTGGCTTCTTGAGGCTTTTGT 6 0.15 No Hit CCCTACTTGAAGTGAATGGGTACAACAAAATCCACAAATACCATACAAGC 6 0.15 No Hit CTGGTGTAGGGGGGAGGTCTCTGTGGAATTTCCATTGCTTCTTGAACATG 6 0.15 No Hit CTCCACTAATAATTCTCTCACAATAATTGCACTTCACTCTTTTCTTCTTT 6 0.15 No Hit GCAATGATGGATGTGAGATCTTTCTTTTTCTATTGGAATTTACTCCAACG 5 0.125 No Hit CAGTTTATCTTCGCGTTTCTTCCTAGTGGAATCTTGGCCCAGTATTTTTC 5 0.125 No Hit GTCAGAAAGAAGCCATTCAAGATCAGGTCAAACTTATGGGTGTTGACTTG 5 0.125 No Hit GGAGGGTTATTAAAACAAGAGAGCAAGAAAAAGACTGTGATGTAGACAGG 5 0.125 No Hit ATATAAACAAGTTTGTTGTTTATAAATACTTTGAAAAAGAAGATCAACCC 5 0.125 No Hit CCCAGTTACATAAAATATTGTGGGCATGTAACCTTACTCTAGTCATATTA 5 0.125 No Hit TTTGTCATGAGTGGGCTCAACAAAATTTGCTTCCAGAACACCTAAGGAAA 5 0.125 No Hit CCTGTCAAGCAACTCCAGCTTCAGCTGCATGTCACTATATGCCTGTTTTT 5 0.125 No Hit CTTAACATTAACACCAAGGTGGAGAGCTCTTCTGATGGTTTCAGCACTGT 5 0.125 No Hit CTCCACAGTTAGCTCCTCCGAATCAACTGTCTTTGCAACTTTCTCCATAA 5 0.125 No Hit ATTCAGGGAACGGAGGACAATCATTTGTTATTTCATTGGGCATTCATCAT 5 0.125 No Hit CCTTGGATCCCCGGGCAATGCTCTAACAAAAGAATGGTCCCAATTGAGAT 5 0.125 No Hit GCCTCATCTGCAGCATATTTTTCAACAAGGGGGCGGAAGACTGGATCCTC 5 0.125 No Hit CTTGAAACAATTTATTTTAACTGAATTTAATTTAGATCAAGCGTGGCAGC 5 0.125 No Hit CAGGTACTTCATCAGAATATATTTCTTCAGGATTCTCTAGACCAAGACTT 5 0.125 No Hit GTGGCTAACATGCTTGATTCAGCAAAGAAAAGATAAAAACGAAAGGAAGA 5 0.125 No Hit GCCCGGTAGAACCACGATTCCATTTCAGTTGCACTGTATCACATCGGAAA 5 0.125 No Hit GTCCGGTCCACCTCGTCCACAACACCTTTCAAAGATGCAGCAGCAGCACA 5 0.125 No Hit TGGAGAACCCTTGAGCATACGAAGTAAAGAAGCCTCTGTCTTGGTGGCAA 5 0.125 No Hit CTTCGCTGCTTGATACTTTGATGAAATCTTACTGCTTCTTGGCATGCCTT 5 0.125 No Hit ACACCATACTGTGCGGGGGGACATCTTTTAATACTAGAGAACCAGCAGCT 5 0.125 No Hit CTTTAATTGAAAAGTATTTTGTCTTAAAGTTACCGAACAATGAAAAGCGG 5 0.125 No Hit GTCCAGTCCAAGAGCGCATGTAAATGTCATCTCCGTCATCCATTCCATAG 5 0.125 No Hit CTTTACTGTTGATGGTTCTTCTTTAGCCACTGCTGACTCGGTAGGTTTTT 5 0.125 No Hit CCCATCTTCAGAAACAGCTGGTGACACCAATTCACTACCACCCCAATTAT 5 0.125 No Hit TCCATCACCAAAGACATCCTCTTCTTGCCCGAATTCAGATGAAATGGCAA 5 0.125 No Hit ATCCGAAACTTTCATCTTCTTCTCCAGCTCAGAGAGTTTGGTCTCAGCAA 5 0.125 No Hit CAGTCAAGCAGCCAAATGAAGCATAAAGCTGGAACATAATTCTTCCAAAC 5 0.125 No Hit CTTCTTCAATGATTCAATATCCGTGGCTAGTTCTTTTCTGCTCGACTTGA 5 0.125 No Hit CGGTGGGATGCCTTCCTTGTCTTGAATCTTGGCTTTGACGTTGTCGATGG 5 0.125 No Hit CTTTCCAAGTCAGTCACCTTCTTTGCAGGCTTCTTCAATTCTTCTCTTTG 5 0.125 No Hit CACAGACATTAGCATGTTAAATGTACTCAAGGTTGGGTTTTGAACCAGCT 5 0.125 No Hit CTCCTAATGGCTCAGCCTTGTTAACTATGGATGCAACAGCCTTACTGTGA 5 0.125 No Hit GTGTGATTTAGGGTTGGGCTTGAGAGCATGAACCATATCAGGGAACTTCA 5 0.125 No Hit GTGGCTGCAGAATTAAGTCCTTTCAACCCACTGCAGCAAGCTGCAGGCAC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.0875 0.0 0.0 0.0 0.0 80-81 0.1 0.0 0.0 0.0 0.0 82-83 0.1375 0.0 0.0 0.0 0.0 84-85 0.1875 0.0 0.0 0.0 0.0 86-87 0.25 0.0 0.0 0.0 0.0 88-89 0.30000000000000004 0.0 0.0 0.0 0.0 90-91 0.3375 0.0 0.0 0.0 0.0 92-93 0.4375 0.0 0.0 0.0 0.0 94-95 0.6000000000000001 0.0 0.0 0.0 0.0 96-97 0.7749999999999999 0.0 0.0 0.0 0.0 98-99 0.8875 0.0 0.0 0.0 0.0 100-101 1.0625 0.0 0.0 0.0 0.0 102-103 1.325 0.0 0.0 0.0 0.0 104-105 1.5375 0.0 0.0 0.0 0.0 106-107 1.825 0.0 0.0 0.0 0.0 108-109 2.1125 0.0 0.0 0.0 0.0 110-111 2.375 0.0 0.0 0.0 0.0 112-113 2.8875 0.0 0.0 0.0 0.0 114-115 3.2625 0.0 0.0 0.0 0.0 116-117 3.625 0.0 0.0 0.0 0.0 118-119 3.8875 0.0 0.0 0.0 0.0 120-121 4.2875 0.0 0.0 0.0 0.0 122-123 4.875 0.0 0.0 0.0 0.0 124-125 5.325 0.0 0.0 0.0 0.0 126-127 5.9 0.0 0.0 0.0 0.0 128-129 6.225 0.0 0.0 0.0 0.0 130-131 6.5125 0.0 0.0 0.0 0.0 132-133 6.9875 0.0 0.0 0.0 0.0 134-135 7.300000000000001 0.0 0.0 0.0 0.0 136-137 7.875 0.0 0.0 0.0 0.0 138-139 8.5 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGGGTT 10 0.006830828 145.0 2 GGACCTT 10 0.006830828 145.0 5 AGGGACC 10 0.006830828 145.0 3 TTCTTAA 10 0.006830828 145.0 7 CCTTTGG 10 0.006830828 145.0 8 GGGACCT 10 0.006830828 145.0 4 CTTAATA 10 0.006830828 145.0 9 TGGGTTC 10 0.006830828 145.0 3 GACCTTT 10 0.006830828 145.0 6 CTTTGGA 10 0.006830828 145.0 9 TCTTAAT 10 0.006830828 145.0 8 CCTGGGT 10 0.006830828 145.0 1 GTGCTAC 10 0.006830828 145.0 145 TTCCAAT 20 0.00593511 29.0 130-134 >>END_MODULE SRR13695428 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695428_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.19575 37.0 37.0 37.0 37.0 37.0 2 36.2205 37.0 37.0 37.0 37.0 37.0 3 36.289 37.0 37.0 37.0 37.0 37.0 4 36.336 37.0 37.0 37.0 37.0 37.0 5 36.349 37.0 37.0 37.0 37.0 37.0 6 36.238 37.0 37.0 37.0 37.0 37.0 7 36.3635 37.0 37.0 37.0 37.0 37.0 8 36.37 37.0 37.0 37.0 37.0 37.0 9 36.328 37.0 37.0 37.0 37.0 37.0 10-14 36.3115 37.0 37.0 37.0 37.0 37.0 15-19 36.303900000000006 37.0 37.0 37.0 37.0 37.0 20-24 36.2809 37.0 37.0 37.0 37.0 37.0 25-29 36.215999999999994 37.0 37.0 37.0 37.0 37.0 30-34 36.125800000000005 37.0 37.0 37.0 37.0 37.0 35-39 36.2051 37.0 37.0 37.0 37.0 37.0 40-44 36.156099999999995 37.0 37.0 37.0 37.0 37.0 45-49 36.16155 37.0 37.0 37.0 37.0 37.0 50-54 36.0712 37.0 37.0 37.0 37.0 37.0 55-59 36.1075 37.0 37.0 37.0 37.0 37.0 60-64 36.040800000000004 37.0 37.0 37.0 37.0 37.0 65-69 36.05460000000001 37.0 37.0 37.0 37.0 37.0 70-74 35.9978 37.0 37.0 37.0 37.0 37.0 75-79 36.0413 37.0 37.0 37.0 37.0 37.0 80-84 35.999649999999995 37.0 37.0 37.0 37.0 37.0 85-89 35.929950000000005 37.0 37.0 37.0 37.0 37.0 90-94 35.9319 37.0 37.0 37.0 37.0 37.0 95-99 35.9454 37.0 37.0 37.0 37.0 37.0 100-104 35.918099999999995 37.0 37.0 37.0 37.0 37.0 105-109 35.929899999999996 37.0 37.0 37.0 37.0 37.0 110-114 35.8067 37.0 37.0 37.0 37.0 37.0 115-119 35.8758 37.0 37.0 37.0 37.0 37.0 120-124 35.7231 37.0 37.0 37.0 37.0 37.0 125-129 35.825649999999996 37.0 37.0 37.0 37.0 37.0 130-134 35.638799999999996 37.0 37.0 37.0 37.0 37.0 135-139 35.61395 37.0 37.0 37.0 37.0 37.0 140-144 35.51769999999999 37.0 37.0 37.0 37.0 37.0 145-149 35.4841 37.0 37.0 37.0 37.0 37.0 150-151 35.14125 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 2.0 15 1.0 16 1.0 17 2.0 18 1.0 19 1.0 20 2.0 21 3.0 22 1.0 23 2.0 24 6.0 25 2.0 26 4.0 27 7.0 28 14.0 29 19.0 30 27.0 31 35.0 32 54.0 33 85.0 34 198.0 35 537.0 36 2761.0 37 234.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 27.840481565086534 20.265864058189116 13.017306245297217 38.87634813142714 2 24.975 25.5 34.2 15.325 3 19.425 28.799999999999997 30.275000000000002 21.5 4 21.6 32.6 25.95 19.85 5 25.474999999999998 35.949999999999996 22.425 16.150000000000002 6 20.150000000000002 38.725 23.775 17.349999999999998 7 20.225 20.325 38.775 20.674999999999997 8 19.3 27.05 30.525000000000002 23.125 9 20.95 24.474999999999998 31.7 22.875 10-14 22.5 29.654999999999998 26.919999999999998 20.925 15-19 22.435 29.304999999999996 27.515 20.745 20-24 22.08662598779634 28.82364709412824 28.013404021206362 21.07632289686906 25-29 21.465732866433214 28.684342171085543 28.91445722861431 20.935467733866933 30-34 22.30392156862745 28.931572629051622 27.89115646258503 20.873349339735896 35-39 22.316695008502553 28.123437031109333 28.64859457837351 20.911273382014606 40-44 22.18387354941977 27.060824329731894 29.671868747498998 21.08343337334934 45-49 22.342819986995448 27.59465813034562 28.815085279847946 21.247436602810986 50-54 22.206661998599582 28.588576572971892 28.273482044613385 20.931279383815145 55-59 22.36618309154577 28.07403701850926 28.22911455727864 21.33066533266633 60-64 22.191657497249174 28.20846253876163 27.903371011303392 21.696508952685807 65-69 22.609521904380873 27.855571114222844 28.560712142428486 20.974194838967794 70-74 21.87093546773387 27.87393696848424 28.36418209104552 21.890945472736366 75-79 22.113845538215283 28.51640656262505 27.641056422569026 21.728691476590637 80-84 23.120780195048763 28.172043010752688 27.82195548887222 20.885221305326333 85-89 23.010354659596818 28.097643939772897 27.93757190735831 20.954429493271974 90-94 22.451735520656197 27.858357507252173 28.238471541462438 21.45143543062919 95-99 23.136941082324697 27.70831249374812 28.39351805541662 20.761228368510555 100-104 22.61630815407704 27.863931965982992 28.159079539769884 21.360680340170084 105-109 22.716358179089543 27.87393696848424 28.419209604802404 20.990495247623812 110-114 23.697109132739822 28.04341302390717 27.323196959087724 20.93628088426528 115-119 23.226613306653327 28.704352176088044 27.64382191095548 20.425212606303152 120-124 23.60680340170085 28.43421710855428 27.78389194597299 20.175087543771888 125-129 24.085838627382323 27.762493121904857 27.52238507328298 20.629283177429844 130-134 24.114468681208727 28.31699019411647 27.32139283570142 20.247148288973385 135-139 23.82310270648857 28.95092300765421 27.29501225674121 19.930962029116014 140-144 24.53736120836251 26.973091927578274 28.543563068920676 19.94598379513854 145-149 25.49274637318659 28.044022011005502 26.58329164582291 19.879939969984992 150-151 26.169627220415308 27.695771828871653 27.24543407555667 18.88916687515637 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.0 7 0.0 8 0.5 9 1.5 10 1.0 11 1.0 12 1.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.5 19 0.5 20 0.0 21 0.0 22 0.5 23 1.5 24 3.5 25 3.5 26 4.0 27 3.5 28 8.5 29 16.0 30 28.0 31 38.0 32 28.0 33 37.0 34 66.0 35 68.5 36 89.0 37 123.0 38 165.0 39 210.0 40 205.5 41 213.0 42 249.0 43 261.5 44 283.0 45 290.0 46 255.0 47 222.0 48 213.5 49 191.5 50 145.0 51 116.0 52 115.0 53 98.5 54 60.5 55 48.0 56 37.0 57 25.5 58 20.5 59 20.5 60 13.0 61 4.0 62 3.5 63 1.5 64 1.0 65 1.0 66 1.0 67 1.0 68 0.0 69 0.0 70 0.5 71 0.5 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.325 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.03 25-29 0.05 30-34 0.04 35-39 0.03 40-44 0.04 45-49 0.034999999999999996 50-54 0.03 55-59 0.05 60-64 0.03 65-69 0.02 70-74 0.05 75-79 0.04 80-84 0.025 85-89 0.045 90-94 0.03 95-99 0.03 100-104 0.05 105-109 0.05 110-114 0.03 115-119 0.05 120-124 0.05 125-129 0.045 130-134 0.06 135-139 0.055 140-144 0.03 145-149 0.05 150-151 0.075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.875 #Duplication Level Percentage of deduplicated Percentage of total 1 70.3499079189687 47.75 2 19.152854511970535 26.0 3 6.114180478821362 12.45 4 2.5046040515653774 6.800000000000001 5 1.2523020257826887 4.25 6 0.44198895027624313 1.7999999999999998 7 0.14732965009208102 0.7000000000000001 8 0.0 0.0 9 0.0 0.0 >10 0.036832412523020254 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCCAAAAAATTTCAGAAAATTGTGAATGAAAAGAATTATGAGTCTTTAA 10 0.25 No Hit GAGAAATTCCTCGACACTCCAGTTATAGTTCTTGGTGTCTTCCTCTTAGT 7 0.17500000000000002 No Hit TAGGAACTCTAGAGGGATTCCTTCGTCAGGCTGATAATCTGAGGCAGCAA 7 0.17500000000000002 No Hit AAAGTCTTGACTAAATCAAACCAACTACTTGCAAAAAAAATTTCAATTAT 7 0.17500000000000002 No Hit GCATGATGCCATGTGTACTGCTTGTGAGATGATGGTTGTATGGATGGAGA 7 0.17500000000000002 No Hit TGTTTAATATGGAGAGAATTCTGGTGTTTAACATCTTCTGTTTAGCCCTG 6 0.15 No Hit CTTGATGTGTCCCTAGCTATATCTGTTAGGGCCTTAGGGGTGGTTTGTTT 6 0.15 No Hit CTTGTTTGAAAACATGGGGAGGCAGGCCAGAGAACGTTCAGGCAGCTCAG 6 0.15 No Hit TGCCGTTCAGGTCGAAAGCGAAGGCAAGCTGAATTTGAACAGGCATCTTT 6 0.15 No Hit ATCTCGAGATACAGGTCCATAGAGCGACTTATTTAAAAGAGAGAAGATGC 6 0.15 No Hit GGTGTTTCGCGATAATCAATTTGTACAGGAGAGAATTATGGACTCCAATG 6 0.15 No Hit AAACATTCTATCTCCCCATCAGTCAGTGAAGCATTACAGATTAATGGCAT 6 0.15 No Hit CCATATGCAGTTACTGAGCTATAGAATCACCATGGTATGCTTGCATTTTC 6 0.15 No Hit CACACTGGGGCGAAGCACACTATTATCCACCGTGATGTTAAGACAACTAA 6 0.15 No Hit AACATACTTAAGAGCAGGCCTGAAATATCAGAAATCCATTGTGTGAAGGA 6 0.15 No Hit ATTGCTTAGTGATGCACTAATGCACATCAGACCATCGCTAAAACATGCTC 6 0.15 No Hit CCTTGGCTGGTAAGATCTCTGGCATCAACTATGGCGTGGCTGCTGGCCTC 6 0.15 No Hit CTTAAGGAACCAATACCCGTGAATTTCCTTAACACGGTTGACAGAGAAGT 5 0.125 No Hit CTAAGTTGGGATTCACAGATTGTAGTAAACTGAATGCTTTGAAGAATACT 5 0.125 No Hit CTTTTCCTCAAGTTCTTAAAGCTCACTCTTTTCTGTCCGTCCGATCAAAT 5 0.125 No Hit CAGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 5 0.125 No Hit TCTATATCCACCACTTGGTTTCTCCTTGCAAAACACACAAGAAAAGAAGG 5 0.125 No Hit TCTGGTTTTCCTAGTTTAATAGAGTTCCCAAATGGGTTACCTCCAGCTCC 5 0.125 No Hit ATTTCCTTAGAGCCCCTGGAGTCCAGACCCCTGTTATTGTCAGGTTTTCT 5 0.125 No Hit ACAGGAAGTACCTGAGAAATCAAAAGATGCTGCAGGTGCACCATGGCAGC 5 0.125 No Hit CATGTGGCCAGGTGTCAAGCAGCTTGGCACAATGTATAACCTACCTCCAG 5 0.125 No Hit TTATAAGCTTGTAAGAGATGGCTTCCTCCTCTATGATCTCATCGGCAGCC 5 0.125 No Hit CAACAGGTGGTGGTGATGAGGTTGTAGACAAGGGTGTTGCTGTGGAGGAA 5 0.125 No Hit TGATTCCACACTAGTTAACTGGTCATTAATTTCAGATAGCATAATATAGA 5 0.125 No Hit CATTTGCCCTCATAAACCAGAAGGTTCTATGTTTGTAATGGTAAAATTGA 5 0.125 No Hit GTTCACCATTGGCTGCAGCAGCAGCCATAGTGAGAATTGGGGCTGAGGCT 5 0.125 No Hit AGTAACTAGTTTTACAAAAGCAGAATGTTGCAACACCGGAACACCAGAAG 5 0.125 No Hit AGAAAGAGCTGCAACAGGAGCGGGACAATGTTAGAGCTCTCACATCTGAC 5 0.125 No Hit GGGTATTGATGAATTTGAGGTGGGCTGTGAATTTTCCTGGTGAGTTGGGA 5 0.125 No Hit CCAGAAGGTCGCTTGCCTGATGCAACCAAAGGTTCAGACCATTTAAGGGA 5 0.125 No Hit CGGCTTTGGTAAAAGTTGGCATTCGCCTCATCACGCTGTTTCCTAAGTTC 5 0.125 No Hit TGTTTAGATTTGCTTGAAGATATGGAAAGGAGGGGTTTGTTAGACATGAA 5 0.125 No Hit GCTGCAGCTATGGTTGCTGCTCATGTTTCTGAACTATTTAGACGTTCAAA 5 0.125 No Hit TGGAACCATGACCATCCTCCACATGAAAATGATTTGGAAAATCTCACTTT 5 0.125 No Hit CTATCATCAATTCTAATTGGTGCCGTCAACTCAGTACCTTTGCTTCCCAA 5 0.125 No Hit CCCTAACACTGCCAAAGAACCAGGAAAAAGAAAAGGAGAGGCAGAGACAC 5 0.125 No Hit GTTCCTGGGGTGGCTGCTGTTGAGCTTTCAATGGTGCCAGGGTCTCATGT 5 0.125 No Hit CGATGGTCTAACAGAAGAGCTTGAGGCTAACTTAAACAGAAAAAAGTCTG 5 0.125 No Hit CGATCACTATAAGTTGCAATGCTTCTTGTTTTAAAATAATTTCATTGTTT 5 0.125 No Hit CATATTGTTCTGCTCTATTGTATCTCAAGGGATACCATTCCCTGCAATCC 5 0.125 No Hit AGTACCCCGCCCTCGCAGTTTCTTCCCCTCCATTTCACGCACAGAGAGAG 5 0.125 No Hit CCATGATCGCAGAGACAACCATCCTCTGGAAACCATAAGAGATCACCACC 5 0.125 No Hit ATATAAAGTGCATCGATTTTAATAATCTGGGACAATCCCGCCTACAAGGA 5 0.125 No Hit GTGAAGAATGTCTTCTTTGTATATTTTTGTAAAATACTAGGTTTCTACCT 5 0.125 No Hit CAAAAATATCGTAGGGTTTCTCAATGGCTGCTGAAGTTGAGTACAGGTGC 5 0.125 No Hit AAATGCTATGGCGCTTGCCCTGAGTCCAGTTGTGAAGGCTCTTGTTGATC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.0875 0.0 0.0 0.0 0.0 80-81 0.1 0.0 0.0 0.0 0.0 82-83 0.1375 0.0 0.0 0.0 0.0 84-85 0.1875 0.0 0.0 0.0 0.0 86-87 0.25 0.0 0.0 0.0 0.0 88-89 0.30000000000000004 0.0 0.0 0.0 0.0 90-91 0.3375 0.0 0.0 0.0 0.0 92-93 0.45 0.0 0.0 0.0 0.0 94-95 0.625 0.0 0.0 0.0 0.0 96-97 0.8 0.0 0.0 0.0 0.0 98-99 0.9375 0.0 0.0 0.0 0.0 100-101 1.1124999999999998 0.0 0.0 0.0 0.0 102-103 1.3625 0.0 0.0 0.0 0.0 104-105 1.5625 0.0 0.0 0.0 0.0 106-107 1.85 0.0 0.0 0.0 0.0 108-109 2.1375 0.0 0.0 0.0 0.0 110-111 2.4000000000000004 0.0 0.0 0.0 0.0 112-113 2.9125 0.0 0.0 0.0 0.0 114-115 3.2875 0.0 0.0 0.0 0.0 116-117 3.65 0.0 0.0 0.0 0.0 118-119 3.9250000000000003 0.0 0.0 0.0 0.0 120-121 4.3375 0.0 0.0 0.0 0.0 122-123 4.975 0.0 0.0 0.0 0.0 124-125 5.425 0.0 0.0 0.0 0.0 126-127 6.0 0.0 0.0 0.0 0.0 128-129 6.3375 0.0 0.0 0.0 0.0 130-131 6.6375 0.0 0.0 0.0 0.0 132-133 7.125 0.0 0.0 0.0 0.0 134-135 7.475 0.0 0.0 0.0 0.0 136-137 8.037500000000001 0.0 0.0 0.0 0.0 138-139 8.662500000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAAGATA 10 0.006830828 145.0 145 AAGTAGC 10 0.006830828 145.0 6 AGTAGCA 10 0.006830828 145.0 7 AATCAAG 10 0.006830828 145.0 5 TATGCAA 10 0.006830828 145.0 9 CCAGAAG 10 0.006830828 145.0 7 TAGCACA 10 0.006830828 145.0 9 GCCAGAA 10 0.006830828 145.0 6 TACTGCC 10 0.006830828 145.0 2 CTGCCAG 10 0.006830828 145.0 4 GTAGCAC 10 0.006830828 145.0 8 CAAGTAG 10 0.006830828 145.0 5 >>END_MODULE Read 960339 spots for SRR13695428.sra Written 960339 spots for SRR13695428.sra Read 960339 spots for SRR13695428.sra Written 960339 spots for SRR13695428.sra Read 960339 spots for SRR13695428.sra Written 960339 spots for SRR13695428.sra Read 960339 spots for SRR13695428.sra Written 960339 spots for SRR13695428.sra Read 960339 spots for SRR13695428.sra Written 960339 spots for SRR13695428.sra Read 960339 spots for SRR13695428.sra Written 960339 spots for SRR13695428.sra Read 960339 spots for SRR13695428.sra Written 960339 spots for SRR13695428.sra Read 960339 spots for SRR13695428.sra Written 960339 spots for SRR13695428.sra Read 960339 spots for SRR13695428.sra Written 960339 spots for SRR13695428.sra Read 960339 spots for SRR13695428.sra Written 960339 spots for SRR13695428.sra Read 960339 spots for SRR13695428.sra Written 960339 spots for SRR13695428.sra Read 960339 spots for SRR13695428.sra Written 960339 spots for SRR13695428.sra Read 960339 spots for SRR13695428.sra Written 960339 spots for SRR13695428.sra Read 960350 spots for SRR13695428.sra Written 960350 spots for SRR13695428.sra Read 960339 spots for SRR13695428.sra Written 960339 spots for SRR13695428.sra Read 960339 spots for SRR13695428.sra Written 960339 spots for SRR13695428.sra Read 960339 spots for SRR13695428.sra Written 960339 spots for SRR13695428.sra Read 960339 spots for SRR13695428.sra Written 960339 spots for SRR13695428.sra Read 960339 spots for SRR13695428.sra Written 960339 spots for SRR13695428.sra Read 960339 spots for SRR13695428.sra Written 960339 spots for SRR13695428.sra SRR ids: ['SRR13695428.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_7tnd00lx SRR13695428.sra spots: 19206791 blocks: [[1, 960339], [960340, 1920678], [1920679, 2881017], [2881018, 3841356], [3841357, 4801695], [4801696, 5762034], [5762035, 6722373], [6722374, 7682712], [7682713, 8643051], [8643052, 9603390], [9603391, 10563729], [10563730, 11524068], [11524069, 12484407], [12484408, 13444746], [13444747, 14405085], [14405086, 15365424], [15365425, 16325763], [16325764, 17286102], [17286103, 18246441], [18246442, 19206791]] SRR13695428 file size 6505607 SRR13695428 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695428 SRR13695428_1.fastq SRR13695428_2.fastq Input file: SRR13695428_1.fastq Paired file: SRR13695428_2.fastq trimmed: SRR13695428-trimmed-pair1.fastq, SRR13695428-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 01:55:19 2025 >> started Wed Feb 12 01:55:41 2025 >> done (22.228s) 19206791 read pairs processed; of these: 114 ( 0.00%) short read pairs filtered out after trimming by size control 1322 ( 0.01%) empty read pairs filtered out after trimming by size control 19205355 (99.99%) read pairs available; of these: 2459005 (12.80%) trimmed read pairs available after processing 16746350 (87.20%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 1 0.00% 20 0 0.00% 21 2 0.00% 22 3 0.00% 23 1 0.00% 24 2 0.00% 25 0 0.00% 26 1 0.00% 27 6 0.00% 28 2 0.00% 29 2 0.00% 30 5 0.00% 31 5 0.00% 32 6 0.00% 33 2 0.00% 34 9 0.00% 35 3 0.00% 36 12 0.00% 37 9 0.00% 38 5 0.00% 39 12 0.00% 40 17 0.00% 41 23 0.00% 42 11 0.00% 43 27 0.00% 44 17 0.00% 45 33 0.00% 46 39 0.00% 47 42 0.00% 48 71 0.00% 49 62 0.00% 50 78 0.00% 51 74 0.00% 52 107 0.00% 53 123 0.00% 54 139 0.00% 55 136 0.00% 56 164 0.00% 57 177 0.00% 58 193 0.00% 59 247 0.00% 60 292 0.00% 61 362 0.00% 62 400 0.00% 63 450 0.00% 64 523 0.00% 65 567 0.00% 66 640 0.00% 67 735 0.00% 68 892 0.00% 69 1048 0.01% 70 1142 0.01% 71 1315 0.01% 72 1502 0.01% 73 1706 0.01% 74 1971 0.01% 75 2200 0.01% 76 2421 0.01% 77 2819 0.01% 78 3120 0.02% 79 3406 0.02% 80 3724 0.02% 81 4267 0.02% 82 4783 0.02% 83 5409 0.03% 84 6092 0.03% 85 6772 0.04% 86 7154 0.04% 87 7705 0.04% 88 8413 0.04% 89 8863 0.05% 90 9572 0.05% 91 10473 0.05% 92 11161 0.06% 93 11835 0.06% 94 13367 0.07% 95 14095 0.07% 96 14857 0.08% 97 15657 0.08% 98 16433 0.09% 99 17407 0.09% 100 18582 0.10% 101 19063 0.10% 102 19810 0.10% 103 21230 0.11% 104 22242 0.12% 105 23113 0.12% 106 24737 0.13% 107 25295 0.13% 108 26235 0.14% 109 27913 0.15% 110 27590 0.14% 111 28811 0.15% 112 30337 0.16% 113 31286 0.16% 114 31949 0.17% 115 33483 0.17% 116 34416 0.18% 117 35672 0.19% 118 37447 0.19% 119 37877 0.20% 120 39084 0.20% 121 40133 0.21% 122 41428 0.22% 123 41861 0.22% 124 43266 0.23% 125 44066 0.23% 126 45738 0.24% 127 47050 0.24% 128 47313 0.25% 129 48215 0.25% 130 49638 0.26% 131 50343 0.26% 132 50793 0.26% 133 51927 0.27% 134 52530 0.27% 135 53665 0.28% 136 55705 0.29% 137 56685 0.30% 138 57281 0.30% 139 59329 0.31% 140 59252 0.31% 141 59228 0.31% 142 60921 0.32% 143 61852 0.32% 144 62568 0.33% 145 63129 0.33% 146 64267 0.33% 147 65021 0.34% 148 66613 0.35% 149 67211 0.35% 150 68385 0.36% 151 16746350 87.20% 19205355 reads passed initial QC criterion=sequence-density sequence-density=0.35 sequence-density-rank=1 fanout-score=2.30 fanout-score-rank=21 prefix-density=0.36 prefix-fanout=2.2 sequence=TGAGATGCCTCAGTGCATCCAAACATGGGTAG criterion=fanout-score sequence-density=0.01 sequence-density-rank=29 fanout-score=589.62 fanout-score-rank=1 prefix-density=0.27 prefix-fanout=18.4 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGAGTATGAATGTGTTCTCTGT criterion=sequence-density sequence-density=0.52 sequence-density-rank=1 fanout-score=2.06 fanout-score-rank=30 prefix-density=0.52 prefix-fanout=2.0 sequence=TGTAAGAGATGGCTTCCTC criterion=fanout-score sequence-density=0.13 sequence-density-rank=18 fanout-score=38.66 fanout-score-rank=1 prefix-density=0.41 prefix-fanout=12.6 sequence=AAAGAAAAGAAAA SRR13695428 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 01:56:20 Started mapping on | Feb 12 01:56:20 Finished on | Feb 12 01:58:07 Mapping speed, Million of reads per hour | 646.16 Number of input reads | 19205355 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 18254536 Uniquely mapped reads % | 95.05% Average mapped length | 294.33 Number of splices: Total | 17952870 Number of splices: Annotated (sjdb) | 17570227 Number of splices: GT/AG | 17591116 Number of splices: GC/AG | 292977 Number of splices: AT/AC | 9858 Number of splices: Non-canonical | 58919 Mismatch rate per base, % | 0.37% Deletion rate per base | 0.03% Deletion average length | 3.04 Insertion rate per base | 0.02% Insertion average length | 2.39 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 429171 % of reads mapped to multiple loci | 2.23% Number of reads mapped to too many loci | 79622 % of reads mapped to too many loci | 0.41% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.20% % of reads unmapped: other | 0.10% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 521899 521899 521899 N_multimapping 429171 429171 429171 N_noFeature 736620 17856912 985843 N_ambiguous 255297 1503 105843 UnstrandedReadsAssigned:17262619 PositiveStrandReadsAssigned:396121 NegativeStrandReadsAssigned:17162850 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695428 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695428-trimmed-pair1.fastq SRR13695428-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 19,205,355 reads, 17,186,874 reads pseudoaligned [quant] estimated average fragment length: 245.473 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,028 rounds 52401 SRR13695428.ke.tsv 34699 SRR13695428.se.tsv 87100 total ==> SRR13695428.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1773.53 504 16.2544 Potri.005G024800.1.v4.1 1035 790.527 182 13.1684 Potri.004G059700.1.v4.1 961 716.632 8 0.638516 Potri.007G009000.2.v4.1 1416 1171.53 0 0 Potri.003G141000.2.v4.1 2943 2698.53 956.329 20.2702 Potri.016G087400.1.v4.1 270 87.1162 868 569.9 Potri.015G069301.1.v4.1 564 328.544 0 0 Potri.010G195200.1.v4.1 1773 1528.53 88 3.29297 Potri.012G127500.1.v4.1 977 732.58 101 7.88576 ==> SRR13695428.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 398 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 224 Potri.001G212900.v4.1 1 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 8 SRR13695428 completed mapping pipeline successfully