Starting /dee2/code/volunteer_pipeline.sh SRR13695430 current disk space = 3050120867840 free memory = 1410795212 SRR13695430 SRAfilesize 617f90596576b866ea2360ed402c8b79 SRR13695430.sra SRR13695430.sra file validated SRR13695430 is paired end SRR13695430 is conventional basespace SRR13695430 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695430_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.6695 37.0 37.0 37.0 37.0 37.0 2 36.2245 37.0 37.0 37.0 37.0 37.0 3 36.5165 37.0 37.0 37.0 37.0 37.0 4 36.436 37.0 37.0 37.0 37.0 37.0 5 36.5635 37.0 37.0 37.0 37.0 37.0 6 36.59 37.0 37.0 37.0 37.0 37.0 7 36.419 37.0 37.0 37.0 37.0 37.0 8 36.55 37.0 37.0 37.0 37.0 37.0 9 36.579 37.0 37.0 37.0 37.0 37.0 10-14 36.5461 37.0 37.0 37.0 37.0 37.0 15-19 36.510000000000005 37.0 37.0 37.0 37.0 37.0 20-24 36.5224 37.0 37.0 37.0 37.0 37.0 25-29 36.4295 37.0 37.0 37.0 37.0 37.0 30-34 36.4388 37.0 37.0 37.0 37.0 37.0 35-39 36.42550000000001 37.0 37.0 37.0 37.0 37.0 40-44 36.4103 37.0 37.0 37.0 37.0 37.0 45-49 36.4106 37.0 37.0 37.0 37.0 37.0 50-54 36.3843 37.0 37.0 37.0 37.0 37.0 55-59 36.372 37.0 37.0 37.0 37.0 37.0 60-64 36.3786 37.0 37.0 37.0 37.0 37.0 65-69 36.3199 37.0 37.0 37.0 37.0 37.0 70-74 36.30239999999999 37.0 37.0 37.0 37.0 37.0 75-79 36.2706 37.0 37.0 37.0 37.0 37.0 80-84 36.209 37.0 37.0 37.0 37.0 37.0 85-89 36.2548 37.0 37.0 37.0 37.0 37.0 90-94 36.129400000000004 37.0 37.0 37.0 37.0 37.0 95-99 36.114 37.0 37.0 37.0 37.0 37.0 100-104 36.1185 37.0 37.0 37.0 37.0 37.0 105-109 36.1061 37.0 37.0 37.0 37.0 37.0 110-114 36.0457 37.0 37.0 37.0 37.0 37.0 115-119 36.0443 37.0 37.0 37.0 37.0 37.0 120-124 36.009299999999996 37.0 37.0 37.0 37.0 37.0 125-129 35.931900000000006 37.0 37.0 37.0 37.0 37.0 130-134 36.0006 37.0 37.0 37.0 37.0 37.0 135-139 35.9084 37.0 37.0 37.0 37.0 37.0 140-144 35.8007 37.0 37.0 37.0 37.0 37.0 145-149 35.6485 37.0 37.0 37.0 37.0 37.0 150-151 35.427499999999995 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 0.0 22 0.0 23 0.0 24 1.0 25 2.0 26 3.0 27 5.0 28 23.0 29 30.0 30 24.0 31 26.0 32 53.0 33 67.0 34 114.0 35 357.0 36 2950.0 37 344.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.95 12.9 5.7 35.449999999999996 2 18.467336683417084 13.467336683417086 37.814070351758794 30.251256281407034 3 16.1 18.275 27.474999999999998 38.15 4 21.475 25.7 24.65 28.175 5 24.95 29.349999999999998 24.224999999999998 21.475 6 21.375 33.35 23.1 22.175 7 14.725 26.625 43.974999999999994 14.674999999999999 8 17.575 22.925 35.325 24.175 9 18.05 21.675 36.175000000000004 24.099999999999998 10-14 19.42 29.875 27.33 23.375 15-19 19.875 29.385 27.875 22.865 20-24 20.28 28.7 27.62 23.400000000000002 25-29 19.725 29.18 27.605 23.49 30-34 20.39 28.33 27.700000000000003 23.580000000000002 35-39 20.215 27.655 28.24 23.89 40-44 19.645000000000003 29.134999999999998 27.87 23.35 45-49 19.79 28.705000000000002 27.525 23.98 50-54 19.665 28.884999999999998 27.375 24.075 55-59 20.51 28.904999999999998 27.315 23.27 60-64 19.950000000000003 27.685 29.160000000000004 23.205000000000002 65-69 20.65 28.810000000000002 27.389999999999997 23.150000000000002 70-74 20.005 29.354999999999997 27.084999999999997 23.555 75-79 20.5 29.225 27.0 23.275000000000002 80-84 20.355 28.585 27.72 23.34 85-89 20.755000000000003 28.449999999999996 27.1 23.695 90-94 19.99 29.2 27.310000000000002 23.5 95-99 20.41 28.43 27.415 23.745 100-104 19.79 28.665000000000003 28.299999999999997 23.244999999999997 105-109 20.205000000000002 28.444999999999997 28.21 23.14 110-114 20.74 28.46 26.97 23.830000000000002 115-119 20.705000000000002 30.175 26.35 22.770000000000003 120-124 20.630000000000003 28.199999999999996 26.83 24.34 125-129 19.42 28.305000000000003 27.485 24.79 130-134 20.845 29.085 26.284999999999997 23.785 135-139 20.794999999999998 28.37 26.745 24.09 140-144 21.11 29.054999999999996 25.965 23.87 145-149 20.89 28.904999999999998 26.57 23.635 150-151 20.65 29.475 25.7625 24.1125 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 1.0 21 1.0 22 1.5 23 2.5 24 3.0 25 2.5 26 4.0 27 8.0 28 10.5 29 11.0 30 12.0 31 21.5 32 30.0 33 41.0 34 52.0 35 64.5 36 83.5 37 103.0 38 147.0 39 166.5 40 172.0 41 232.5 42 259.5 43 249.0 44 273.0 45 306.5 46 311.5 47 280.5 48 224.5 49 176.5 50 158.5 51 127.0 52 89.5 53 75.0 54 66.5 55 60.5 56 49.5 57 34.0 58 25.0 59 21.0 60 14.0 61 5.0 62 4.5 63 5.0 64 3.0 65 2.5 66 2.0 67 0.5 68 0.5 69 1.0 70 0.5 71 0.0 72 0.0 73 0.0 74 1.0 75 1.5 76 0.5 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.5 #Duplication Level Percentage of deduplicated Percentage of total 1 71.29496402877697 49.55 2 18.633093525179856 25.900000000000002 3 7.086330935251799 14.774999999999999 4 1.8345323741007193 5.1 5 0.7194244604316548 2.5 6 0.14388489208633093 0.6 7 0.14388489208633093 0.7000000000000001 8 0.07194244604316546 0.4 9 0.03597122302158273 0.22499999999999998 >10 0.03597122302158273 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACTCGGTTCATCTATCAGATAAATGTCAGCTGGCTTTCCAAGGCATAGAC 10 0.25 No Hit CTACCATTCCTCCCAATATGAAACCGCCAATGAAGATACCTGCACCACTT 9 0.22499999999999998 No Hit GCTTGAAGTTGTTCTCCTTCTCAATGATGCACTTCTCACGCTTTCCACTG 8 0.2 No Hit CCCTGTATTAATCAGGCCACGGGGTAGCAAACTCTTAACAGCAGGAAGAG 8 0.2 No Hit GTCCAAGTCATCGTAGTCATATTCATTTTCCGCTCCGTGACTAAGATTGG 7 0.17500000000000002 No Hit GGCAGATTCTCCTTCCAGAACCAAATGGGATGAATTCAAAATTGGCACCT 7 0.17500000000000002 No Hit GTACTGTTTATACTTGGGAATTCCTAGCTCAAGCCATGGTTTCATTTGTC 7 0.17500000000000002 No Hit GATGGCAGCATAACAGGCAACAACTACACGCTAGTGGAAATTACTCTGCT 7 0.17500000000000002 No Hit GCTTGGAAACCGACAGAGACCATAAAACACCATCCATTTATAGTCATGCA 6 0.15 No Hit ATCCGTTCGAGATTGTACAGCACCATCTCCTCGAGAGCCGATTGATAGGA 6 0.15 No Hit GCCACCATTGATTCCATAAGGCTGATCAGTCTTGGACTTCCTCGAACGAC 6 0.15 No Hit CTAACTTCCAAAACTTAGTTCAAGTCAAACTCGTACATCTAAACCAAGTC 6 0.15 No Hit GTTGCAAACACTCTGGTTTTTAATTTTATTTGTGGTAATGTTACAAAAGC 5 0.125 No Hit GTCCTTAAGAAGTTGATGATAGGGTTTATGTATGAACTTGCACTGTCACT 5 0.125 No Hit GTCCGGAACACATTGCAATGCTGATATATCATCACCAACCACTTCTTGCT 5 0.125 No Hit CACACCCCCAACAAAACCCATCCCACTTATTTATTTTTCTCACTAGGACC 5 0.125 No Hit GCCGCAAGAGCTTCAAGTCCAACGTCTTCAATGTAATCCAGCACCCATAA 5 0.125 No Hit CTGGGTTTATCAAACAAATAAAGTGTTGCAGATCAGATTACTCGGTTTGT 5 0.125 No Hit CCCTACGCCACACACATGACGGTTTACGTGCTTAATACGTGCTTAATGAC 5 0.125 No Hit AGCAGCTGAGCGGGAGGCAAGTCCATCAAGACCTTGGCACCACGACTCGT 5 0.125 No Hit GTTCAAGATCTCCGTGCACCATAGCTGCAGAAAAATCAGACTGGTATGAG 5 0.125 No Hit GTCAAATAGATGGTCTATATTCTCGAACACTTGGATATCAGCATCCAAAT 5 0.125 No Hit GCCCCACTTCTTCGTTGATTCCTTCTTAGTCCTCCTTGAATTAGGATCGA 5 0.125 No Hit TCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGC 5 0.125 No Hit ACCTTGCTAGCTTCTCTGTCTTCCACCACATATTCCTTTGTAAGTTCTTG 5 0.125 No Hit AGGTGCTTCAATATAATAACAAGCTTTTCACAGTTCACACAGCAGAAGCC 5 0.125 No Hit CCTGGATCTGTATTCTGAATCACCTGGCTTTCACCCTTTTCAAGGGTTTG 5 0.125 No Hit CCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGC 5 0.125 No Hit CTCCCCCTCTGTCTCATACCTCTTTCCATGCCTGTGAGCAAAACGAGCAA 5 0.125 No Hit GCTTCTTGTCAACTCAAATGTAATCTTCTTGGCCTCCTCCATGAGAAGAA 5 0.125 No Hit ACTCTTAAACGCATTTTGAAACTCATCCATAAGTATAGCCCTGATTTTCT 5 0.125 No Hit GCCATGCTCAAATAACAGCTCAAAACCAAGGATTTTCAAGATCCTGCATA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0125 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.125 0.0 0.0 0.0 0.0 82-83 0.1375 0.0 0.0 0.0 0.0 84-85 0.2 0.0 0.0 0.0 0.0 86-87 0.4125 0.0 0.0 0.0 0.0 88-89 0.6125 0.0 0.0 0.0 0.0 90-91 0.7625 0.0 0.0 0.0 0.0 92-93 0.8 0.0 0.0 0.0 0.0 94-95 0.9875 0.0 0.0 0.0 0.0 96-97 1.1125 0.0 0.0 0.0 0.0 98-99 1.2625 0.0 0.0 0.0 0.0 100-101 1.4625 0.0 0.0 0.0 0.0 102-103 1.575 0.0 0.0 0.0 0.0 104-105 1.7625 0.0 0.0 0.0 0.0 106-107 2.0375 0.0 0.0 0.0 0.0 108-109 2.2249999999999996 0.0 0.0 0.0 0.0 110-111 2.675 0.0 0.0 0.0 0.0 112-113 2.9375 0.0 0.0 0.0 0.0 114-115 3.25 0.0 0.0 0.0 0.0 116-117 3.45 0.0 0.0 0.0 0.0 118-119 3.7625 0.0 0.0 0.0 0.0 120-121 4.275 0.0 0.0 0.0 0.0 122-123 4.6875 0.0 0.0 0.0 0.0 124-125 5.199999999999999 0.0 0.0 0.0 0.0 126-127 5.6625 0.0 0.0 0.0 0.0 128-129 6.1875 0.0 0.0 0.0 0.0 130-131 6.825 0.0 0.0 0.0 0.0 132-133 7.3625 0.0 0.0 0.0 0.0 134-135 8.05 0.0 0.0 0.0 0.0 136-137 8.675 0.0 0.0 0.0 0.0 138-139 9.3125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR13695430 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695430_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.12225 37.0 37.0 37.0 37.0 37.0 2 36.327 37.0 37.0 37.0 37.0 37.0 3 36.2255 37.0 37.0 37.0 37.0 37.0 4 36.2455 37.0 37.0 37.0 37.0 37.0 5 36.2845 37.0 37.0 37.0 37.0 37.0 6 36.314 37.0 37.0 37.0 37.0 37.0 7 36.4 37.0 37.0 37.0 37.0 37.0 8 36.32 37.0 37.0 37.0 37.0 37.0 9 36.332 37.0 37.0 37.0 37.0 37.0 10-14 36.359300000000005 37.0 37.0 37.0 37.0 37.0 15-19 36.303399999999996 37.0 37.0 37.0 37.0 37.0 20-24 36.1948 37.0 37.0 37.0 37.0 37.0 25-29 36.1874 37.0 37.0 37.0 37.0 37.0 30-34 36.18675 37.0 37.0 37.0 37.0 37.0 35-39 36.19590000000001 37.0 37.0 37.0 37.0 37.0 40-44 36.15895 37.0 37.0 37.0 37.0 37.0 45-49 36.136900000000004 37.0 37.0 37.0 37.0 37.0 50-54 36.09585 37.0 37.0 37.0 37.0 37.0 55-59 36.1299 37.0 37.0 37.0 37.0 37.0 60-64 36.0503 37.0 37.0 37.0 37.0 37.0 65-69 36.005250000000004 37.0 37.0 37.0 37.0 37.0 70-74 35.9267 37.0 37.0 37.0 37.0 37.0 75-79 35.9544 37.0 37.0 37.0 37.0 37.0 80-84 35.952149999999996 37.0 37.0 37.0 37.0 37.0 85-89 35.84485 37.0 37.0 37.0 37.0 37.0 90-94 35.8597 37.0 37.0 37.0 37.0 37.0 95-99 35.89655 37.0 37.0 37.0 37.0 37.0 100-104 35.826499999999996 37.0 37.0 37.0 37.0 37.0 105-109 35.80905 37.0 37.0 37.0 37.0 37.0 110-114 35.7944 37.0 37.0 37.0 37.0 37.0 115-119 35.712199999999996 37.0 37.0 37.0 37.0 37.0 120-124 35.741400000000006 37.0 37.0 37.0 37.0 37.0 125-129 35.63125 37.0 37.0 37.0 37.0 37.0 130-134 35.4728 37.0 37.0 37.0 37.0 37.0 135-139 35.46065 37.0 37.0 37.0 37.0 37.0 140-144 35.3224 37.0 37.0 37.0 34.6 37.0 145-149 35.176050000000004 37.0 37.0 37.0 29.8 37.0 150-151 35.01275 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 2.0 15 2.0 16 2.0 17 1.0 18 2.0 19 0.0 20 0.0 21 0.0 22 4.0 23 7.0 24 2.0 25 11.0 26 4.0 27 10.0 28 15.0 29 23.0 30 17.0 31 30.0 32 53.0 33 114.0 34 217.0 35 562.0 36 2705.0 37 216.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.8706442717473 24.51742291301078 9.952369014790674 21.659563800451238 2 29.575000000000003 26.0 29.2 15.225 3 22.85 29.049999999999997 30.45 17.65 4 24.7 35.199999999999996 21.75 18.35 5 25.650000000000002 37.1 21.25 16.0 6 21.6 40.9 20.3 17.2 7 19.7 23.7 37.925 18.675 8 19.375 27.125 30.2 23.3 9 22.275 25.775 29.049999999999997 22.900000000000002 10-14 23.62 29.330000000000002 26.305 20.745 15-19 21.935 29.085 27.775 21.205 20-24 23.507052115634693 28.738621586475944 28.19845953786136 19.55586676002801 25-29 23.351675837918958 28.849424712356175 27.953976988494244 19.844922461230617 30-34 22.73795828539989 27.9697894262992 28.429950482668936 20.862301805631972 35-39 23.116935080524158 28.02840852255677 28.67360208062419 20.18105431629489 40-44 22.55789526334217 28.03481218426449 28.304906717351074 21.102385835042263 45-49 22.491747524257278 28.193458037411222 28.55856757027108 20.756226868060416 50-54 22.745686421605402 28.252063015753937 28.017004251062765 20.985246311577892 55-59 23.261630815407706 27.57878939469735 28.36418209104552 20.795397698849424 60-64 22.33670101030309 28.998699609882966 27.9183755126538 20.74622386716015 65-69 22.98344751712757 27.14407161074161 28.294244136620495 21.578236735510327 70-74 22.696348174087046 27.55377688844422 28.54927463731866 21.200600300150075 75-79 23.364345738295317 27.32092837134854 27.81112444977991 21.50360144057623 80-84 22.540635158789698 27.886971742935735 28.962240560140035 20.610152538134532 85-89 23.09539292681707 28.152668700915413 27.962583162423087 20.78935520984443 90-94 23.281984595378614 28.313494048214466 27.48324497349205 20.921276382914876 95-99 23.705926481620406 27.941985496374095 27.536884221055264 20.81520380095024 100-104 23.321660830415208 27.49374687343672 28.809404702351177 20.3751875937969 105-109 23.485568505827622 28.252713721174526 28.052623680656296 20.209094092341555 110-114 23.512053616084824 28.15344603381014 28.27848354506352 20.056016805041512 115-119 24.469787915166066 27.871148459383754 27.761104441776713 19.897959183673468 120-124 24.452226113056525 27.41870935467734 27.743871935967984 20.38519259629815 125-129 24.283499224728654 27.57465112789476 27.634672135247335 20.507177512129246 130-134 25.42025215129077 28.051831098659196 27.171302781669 19.35661396838103 135-139 25.023763069688325 27.094902196207915 28.445645104807642 19.435689629296114 140-144 26.08282484745424 27.498249474842453 27.18815644693408 19.230769230769234 145-149 25.8542198209015 28.230526789734355 27.1699434689079 18.74530992045625 150-151 26.70753064798599 26.99524643482612 26.695021265949464 19.60220165123843 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.5 8 0.5 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.5 18 0.5 19 0.5 20 1.5 21 2.0 22 1.0 23 1.0 24 3.5 25 3.0 26 6.0 27 8.5 28 8.5 29 12.5 30 16.5 31 22.0 32 29.0 33 39.5 34 56.0 35 73.5 36 93.0 37 121.5 38 167.5 39 180.5 40 184.0 41 231.0 42 246.0 43 267.0 44 281.0 45 252.0 46 258.0 47 261.0 48 227.0 49 208.0 50 175.0 51 125.0 52 102.0 53 72.5 54 52.5 55 49.5 56 45.0 57 34.5 58 21.0 59 15.0 60 8.0 61 4.0 62 3.5 63 4.0 64 5.5 65 4.0 66 1.0 67 0.5 68 1.0 69 1.0 70 1.0 71 1.0 72 0.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.5 79 1.0 80 1.0 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 1.0 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27499999999999997 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.03 25-29 0.05 30-34 0.034999999999999996 35-39 0.03 40-44 0.034999999999999996 45-49 0.03 50-54 0.025 55-59 0.05 60-64 0.03 65-69 0.015 70-74 0.05 75-79 0.04 80-84 0.025 85-89 0.045 90-94 0.03 95-99 0.025 100-104 0.05 105-109 0.045 110-114 0.03 115-119 0.04 120-124 0.05 125-129 0.034999999999999996 130-134 0.06 135-139 0.055 140-144 0.03 145-149 0.055 150-151 0.075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.95 #Duplication Level Percentage of deduplicated Percentage of total 1 71.90850607576841 50.3 2 18.334524660471764 25.650000000000002 3 6.719085060757685 14.099999999999998 4 1.9299499642601858 5.4 5 0.6075768406004289 2.125 6 0.25017869907076484 1.05 7 0.14295925661186562 0.7000000000000001 8 0.035739814152966405 0.2 9 0.035739814152966405 0.22499999999999998 >10 0.035739814152966405 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAAACCTGATACTGTAGAGGGTTCCGAAGCCGAGATACCTGAGTTCAATG 10 0.25 No Hit AAAGCAACCATTTTTTAGTGCCATTTCTCCTTTTTTGATTACTCAAACAA 9 0.22499999999999998 No Hit GTGGATGTCTTGAAAACTGAGAGTATCGATTTAACTTCTTTGCACTTGTC 8 0.2 No Hit CTTGTCTTTCACAAGTTTTATGTGAATAAAATGAATACCTCAAAACCAAA 7 0.17500000000000002 No Hit ATAAGAACATCGACAACTTCTTTGCTGAAAATGAGCAGCTTGCTTTCTGC 7 0.17500000000000002 No Hit GTTGTGAAGTTCATGGATATGCCATAGAAGCTAACAGCAAAGTAATGGTG 7 0.17500000000000002 No Hit GTTTTCCTACGTTACAAACATGGAAAACCAGGTAAGCAAGGGAACAGGAT 7 0.17500000000000002 No Hit GGCACCACTTCCGAGGAAGATGATGGCTACCTGATATTCTTCGCACATGA 6 0.15 No Hit CACAGAGAACACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCT 6 0.15 No Hit GTTGGGTTGTAATTTTCAAGCTTGGAGGGGGTTTGTTGGGTGCGGGACTG 6 0.15 No Hit GTGTATATATAGGTAGTGTTTTTTGCATGGTTTGTACAGTGTGGTTGATA 6 0.15 No Hit ATTTCTTTTCTAATTTGACACTGCTTTAAATTTTCCTCATTTCGCTGCTA 6 0.15 No Hit AAGCTATCGAGCTGGTTAAGAGCTCTGGGGGCATTGAAAGGGCGCAGGAA 6 0.15 No Hit ATCAAGCAAAGCTTAAACACTAATTAATCATGGCAACCAGCTCAGTTATG 6 0.15 No Hit CCTGAATATTTCTCCTCCAGATGGGCTATTTAACATGAAAGAGACTGCTT 5 0.125 No Hit AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 5 0.125 No Hit AAGAAACGGTACAGGAAAAACAACTTTCCTCAGGTACATGGCTCTGCATG 5 0.125 No Hit CAAGAAGATGGTTGATGTTCTTGTTGAGCAGAAGATTGTTCCTGGTATCA 5 0.125 No Hit ACAAAATCATTGCGGTGCCCCAGCAGAAAAATGACTATGATTGTGGCCTC 5 0.125 No Hit AGCGGGTGAGCCGGTTCGGTTCGTTGTTTACGACCCATGTTTTCGGTGAA 5 0.125 No Hit CCGAGGAACACCGTGACATTTTGAGGTCTCAAGGTTGCATTGTTCGTGAG 5 0.125 No Hit ACCACATATAGAGGGTGTAATAGCTAAGTAGCCTGTAAGAGATGGCTTCC 5 0.125 No Hit GAAAAGAGTGCTAAAATCTTGATTTTTTCAGCAGTTTCTTATATATATAT 5 0.125 No Hit GATATCTTATTGGCTCTCTCTTGGGTTTTATTTCTTGTTTGTTCTTGGAG 5 0.125 No Hit AAACAAGTAAATCATGGCTCGTGTCGCTGGGTTGGTGGTCTCATCGATCT 5 0.125 No Hit ATCCAGCTTACCTTCCAGCAGTGTATCCTGTCTGTTCTGGCTTAACATCA 5 0.125 No Hit GCGAATTTATGATCCGAACAGAGACACACTTGAAGCCCTTAGAGGATCAA 5 0.125 No Hit GGGGAGGAACTAAGGGAAATGTTTGGCTATGAAGAGCATCCCTATGGATT 5 0.125 No Hit TGGGATTTCAGCTGTGACTTCGAAGTAGATTTTGGGTCTGAGGAGAATGC 5 0.125 No Hit GTGTTTTCTAGAAAGGCTTTCTTGATCATATCTCCAACTAATCAAATGAC 5 0.125 No Hit CGGACGAGGGAGGTTCAATCTGTGTGTGAGAACTTCGATAAATGGAGGAT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0125 0.0 60-61 0.0 0.0 0.0 0.025 0.0 62-63 0.0 0.0 0.0 0.025 0.0 64-65 0.0 0.0 0.0 0.025 0.0 66-67 0.0 0.0 0.0 0.025 0.0 68-69 0.0 0.0 0.0 0.025 0.0 70-71 0.0 0.0 0.0 0.025 0.0 72-73 0.0 0.0 0.0 0.025 0.0 74-75 0.0 0.0 0.0 0.025 0.0 76-77 0.0125 0.0 0.0 0.025 0.0 78-79 0.037500000000000006 0.0 0.0 0.025 0.0 80-81 0.1 0.0 0.0 0.025 0.0 82-83 0.1125 0.0 0.0 0.025 0.0 84-85 0.175 0.0 0.0 0.025 0.0 86-87 0.38749999999999996 0.0 0.0 0.025 0.0 88-89 0.5875 0.0 0.0 0.025 0.0 90-91 0.7375 0.0 0.0 0.025 0.0 92-93 0.775 0.0 0.0 0.025 0.0 94-95 0.9624999999999999 0.0 0.0 0.025 0.0 96-97 1.0875 0.0 0.0 0.025 0.0 98-99 1.2375 0.0 0.0 0.025 0.0 100-101 1.4375 0.0 0.0 0.025 0.0 102-103 1.5625 0.0 0.0 0.025 0.0 104-105 1.7625 0.0 0.0 0.025 0.0 106-107 2.0375 0.0 0.0 0.025 0.0 108-109 2.2249999999999996 0.0 0.0 0.025 0.0 110-111 2.675 0.0 0.0 0.025 0.0 112-113 2.95 0.0 0.0 0.025 0.0 114-115 3.275 0.0 0.0 0.025 0.0 116-117 3.4749999999999996 0.0 0.0 0.025 0.0 118-119 3.7874999999999996 0.0 0.0 0.025 0.0 120-121 4.2875 0.0 0.0 0.025 0.0 122-123 4.6875 0.0 0.0 0.025 0.0 124-125 5.199999999999999 0.0 0.0 0.025 0.0 126-127 5.65 0.0 0.0 0.025 0.0 128-129 6.1625 0.0 0.0 0.025 0.0 130-131 6.7875 0.0 0.0 0.025 0.0 132-133 7.3375 0.0 0.0 0.025 0.0 134-135 8.05 0.0 0.0 0.025 0.0 136-137 8.6875 0.0 0.0 0.025 0.0 138-139 9.3625 0.0 0.0 0.025 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 936195 spots for SRR13695430.sra Written 936195 spots for SRR13695430.sra Read 936195 spots for SRR13695430.sra Written 936195 spots for SRR13695430.sra Read 936195 spots for SRR13695430.sra Written 936195 spots for SRR13695430.sra Read 936195 spots for SRR13695430.sra Written 936195 spots for SRR13695430.sra Read 936195 spots for SRR13695430.sra Written 936195 spots for SRR13695430.sra Read 936195 spots for SRR13695430.sra Written 936195 spots for SRR13695430.sra Read 936195 spots for SRR13695430.sra Written 936195 spots for SRR13695430.sra Read 936195 spots for SRR13695430.sra Written 936195 spots for SRR13695430.sra Read 936195 spots for SRR13695430.sra Written 936195 spots for SRR13695430.sra Read 936195 spots for SRR13695430.sra Written 936195 spots for SRR13695430.sra Read 936195 spots for SRR13695430.sra Written 936195 spots for SRR13695430.sra Read 936195 spots for SRR13695430.sra Written 936195 spots for SRR13695430.sra Read 936195 spots for SRR13695430.sra Written 936195 spots for SRR13695430.sra Read 936195 spots for SRR13695430.sra Written 936195 spots for SRR13695430.sra Read 936195 spots for SRR13695430.sra Written 936195 spots for SRR13695430.sra Read 936206 spots for SRR13695430.sra Written 936206 spots for SRR13695430.sra Read 936195 spots for SRR13695430.sra Written 936195 spots for SRR13695430.sra Read 936195 spots for SRR13695430.sra Written 936195 spots for SRR13695430.sra Read 936195 spots for SRR13695430.sra Written 936195 spots for SRR13695430.sra Read 936195 spots for SRR13695430.sra Written 936195 spots for SRR13695430.sra SRR ids: ['SRR13695430.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_cmnh0bn7 SRR13695430.sra spots: 18723911 blocks: [[1, 936195], [936196, 1872390], [1872391, 2808585], [2808586, 3744780], [3744781, 4680975], [4680976, 5617170], [5617171, 6553365], [6553366, 7489560], [7489561, 8425755], [8425756, 9361950], [9361951, 10298145], [10298146, 11234340], [11234341, 12170535], [12170536, 13106730], [13106731, 14042925], [14042926, 14979120], [14979121, 15915315], [15915316, 16851510], [16851511, 17787705], [17787706, 18723911]] SRR13695430 file size 6341503 SRR13695430 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695430 SRR13695430_1.fastq SRR13695430_2.fastq Input file: SRR13695430_1.fastq Paired file: SRR13695430_2.fastq trimmed: SRR13695430-trimmed-pair1.fastq, SRR13695430-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 02:03:23 2025 >> started Wed Feb 12 02:03:55 2025 >> done (31.893s) 18723911 read pairs processed; of these: 147 ( 0.00%) short read pairs filtered out after trimming by size control 2082 ( 0.01%) empty read pairs filtered out after trimming by size control 18721682 (99.99%) read pairs available; of these: 2270921 (12.13%) trimmed read pairs available after processing 16450761 (87.87%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 5 0.00% 19 2 0.00% 20 3 0.00% 21 5 0.00% 22 6 0.00% 23 1 0.00% 24 2 0.00% 25 8 0.00% 26 7 0.00% 27 3 0.00% 28 1 0.00% 29 9 0.00% 30 7 0.00% 31 5 0.00% 32 6 0.00% 33 10 0.00% 34 8 0.00% 35 9 0.00% 36 9 0.00% 37 7 0.00% 38 10 0.00% 39 7 0.00% 40 21 0.00% 41 18 0.00% 42 18 0.00% 43 23 0.00% 44 28 0.00% 45 30 0.00% 46 30 0.00% 47 44 0.00% 48 54 0.00% 49 83 0.00% 50 67 0.00% 51 69 0.00% 52 127 0.00% 53 126 0.00% 54 150 0.00% 55 171 0.00% 56 181 0.00% 57 222 0.00% 58 226 0.00% 59 258 0.00% 60 286 0.00% 61 369 0.00% 62 455 0.00% 63 478 0.00% 64 545 0.00% 65 624 0.00% 66 724 0.00% 67 773 0.00% 68 796 0.00% 69 1061 0.01% 70 1212 0.01% 71 1512 0.01% 72 1630 0.01% 73 1850 0.01% 74 2082 0.01% 75 2292 0.01% 76 2589 0.01% 77 2778 0.01% 78 3094 0.02% 79 3433 0.02% 80 3896 0.02% 81 4358 0.02% 82 5022 0.03% 83 5404 0.03% 84 6203 0.03% 85 6892 0.04% 86 7102 0.04% 87 7437 0.04% 88 8211 0.04% 89 8513 0.05% 90 9615 0.05% 91 10331 0.06% 92 11133 0.06% 93 11882 0.06% 94 12862 0.07% 95 13525 0.07% 96 14472 0.08% 97 15402 0.08% 98 15563 0.08% 99 16598 0.09% 100 17754 0.09% 101 17751 0.09% 102 19564 0.10% 103 19844 0.11% 104 21023 0.11% 105 21717 0.12% 106 23026 0.12% 107 23867 0.13% 108 24027 0.13% 109 25395 0.14% 110 25827 0.14% 111 27174 0.15% 112 28293 0.15% 113 28747 0.15% 114 30009 0.16% 115 30815 0.16% 116 31609 0.17% 117 33347 0.18% 118 34118 0.18% 119 34924 0.19% 120 36196 0.19% 121 37135 0.20% 122 37384 0.20% 123 38439 0.21% 124 39803 0.21% 125 40263 0.22% 126 42158 0.23% 127 42088 0.22% 128 43281 0.23% 129 44294 0.24% 130 45511 0.24% 131 45173 0.24% 132 46382 0.25% 133 47801 0.26% 134 48194 0.26% 135 49737 0.27% 136 50258 0.27% 137 51892 0.28% 138 52085 0.28% 139 53593 0.29% 140 53659 0.29% 141 54582 0.29% 142 54742 0.29% 143 56071 0.30% 144 57562 0.31% 145 57835 0.31% 146 57661 0.31% 147 60163 0.32% 148 61028 0.33% 149 60643 0.32% 150 61397 0.33% 151 16450761 87.87% 18721682 reads passed initial QC criterion=sequence-density sequence-density=0.39 sequence-density-rank=1 fanout-score=1.97 fanout-score-rank=30 prefix-density=0.40 prefix-fanout=1.9 sequence=TGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGG criterion=fanout-score sequence-density=0.01 sequence-density-rank=31 fanout-score=507.98 fanout-score-rank=1 prefix-density=0.27 prefix-fanout=19.6 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAG criterion=sequence-density sequence-density=1.11 sequence-density-rank=1 fanout-score=1.98 fanout-score-rank=31 prefix-density=1.10 prefix-fanout=2.0 sequence=CTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAGTGAACAAACAAAAGTGTCCGTGAGTGTCTTTATCTTTGTGTCTTCTTCA criterion=fanout-score sequence-density=0.10 sequence-density-rank=29 fanout-score=57.74 fanout-score-rank=1 prefix-density=0.69 prefix-fanout=8.2 sequence=AAAAGAAAAGAAAA SRR13695430 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 02:04:54 Started mapping on | Feb 12 02:04:54 Finished on | Feb 12 02:07:29 Mapping speed, Million of reads per hour | 434.83 Number of input reads | 18721682 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 17440700 Uniquely mapped reads % | 93.16% Average mapped length | 294.27 Number of splices: Total | 16994163 Number of splices: Annotated (sjdb) | 16594249 Number of splices: GT/AG | 16637855 Number of splices: GC/AG | 269073 Number of splices: AT/AC | 10752 Number of splices: Non-canonical | 76483 Mismatch rate per base, % | 0.37% Deletion rate per base | 0.03% Deletion average length | 2.95 Insertion rate per base | 0.02% Insertion average length | 2.37 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 408630 % of reads mapped to multiple loci | 2.18% Number of reads mapped to too many loci | 108873 % of reads mapped to too many loci | 0.58% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.92% % of reads unmapped: other | 0.16% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 872560 872560 872560 N_multimapping 408630 408630 408630 N_noFeature 762046 16927450 1104590 N_ambiguous 273992 2117 101645 UnstrandedReadsAssigned:16404662 PositiveStrandReadsAssigned:511133 NegativeStrandReadsAssigned:16234465 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695430 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695430-trimmed-pair1.fastq SRR13695430-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 18,721,682 reads, 16,262,439 reads pseudoaligned [quant] estimated average fragment length: 251.591 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,247 rounds 52401 SRR13695430.ke.tsv 34699 SRR13695430.se.tsv 87100 total ==> SRR13695430.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1767.41 665 20.7707 Potri.005G024800.1.v4.1 1035 784.409 242 17.0309 Potri.004G059700.1.v4.1 961 710.557 4 0.310761 Potri.007G009000.2.v4.1 1416 1165.41 0 0 Potri.003G141000.2.v4.1 2943 2692.41 918.459 18.8315 Potri.016G087400.1.v4.1 270 87.0603 587 372.207 Potri.015G069301.1.v4.1 564 324.234 0 0 Potri.010G195200.1.v4.1 1773 1522.41 71 2.5745 Potri.012G127500.1.v4.1 977 726.484 63 4.78719 ==> SRR13695430.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 101 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 217 Potri.001G212900.v4.1 32 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 2 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR13695430 completed mapping pipeline successfully