Starting /dee2/code/volunteer_pipeline.sh SRR13695431 current disk space = 3050090573824 free memory = 1577504364 SRR13695431 SRAfilesize a104dcd473c26f43522cc10fc0a32be8 SRR13695431.sra SRR13695431.sra file validated SRR13695431 is paired end SRR13695431 is conventional basespace SRR13695431 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695431_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.587 37.0 37.0 37.0 37.0 37.0 2 36.35475 37.0 37.0 37.0 37.0 37.0 3 36.4945 37.0 37.0 37.0 37.0 37.0 4 36.573 37.0 37.0 37.0 37.0 37.0 5 36.571 37.0 37.0 37.0 37.0 37.0 6 36.6495 37.0 37.0 37.0 37.0 37.0 7 36.467 37.0 37.0 37.0 37.0 37.0 8 36.5935 37.0 37.0 37.0 37.0 37.0 9 36.6035 37.0 37.0 37.0 37.0 37.0 10-14 36.5763 37.0 37.0 37.0 37.0 37.0 15-19 36.572500000000005 37.0 37.0 37.0 37.0 37.0 20-24 36.4952 37.0 37.0 37.0 37.0 37.0 25-29 36.4267 37.0 37.0 37.0 37.0 37.0 30-34 36.471000000000004 37.0 37.0 37.0 37.0 37.0 35-39 36.4019 37.0 37.0 37.0 37.0 37.0 40-44 36.4225 37.0 37.0 37.0 37.0 37.0 45-49 36.35170000000001 37.0 37.0 37.0 37.0 37.0 50-54 36.3114 37.0 37.0 37.0 37.0 37.0 55-59 36.3746 37.0 37.0 37.0 37.0 37.0 60-64 36.319 37.0 37.0 37.0 37.0 37.0 65-69 36.30480000000001 37.0 37.0 37.0 37.0 37.0 70-74 36.285700000000006 37.0 37.0 37.0 37.0 37.0 75-79 36.285900000000005 37.0 37.0 37.0 37.0 37.0 80-84 36.1543 37.0 37.0 37.0 37.0 37.0 85-89 36.2282 37.0 37.0 37.0 37.0 37.0 90-94 36.1978 37.0 37.0 37.0 37.0 37.0 95-99 36.09930000000001 37.0 37.0 37.0 37.0 37.0 100-104 36.0855 37.0 37.0 37.0 37.0 37.0 105-109 36.1258 37.0 37.0 37.0 37.0 37.0 110-114 36.060300000000005 37.0 37.0 37.0 37.0 37.0 115-119 36.0393 37.0 37.0 37.0 37.0 37.0 120-124 35.9813 37.0 37.0 37.0 37.0 37.0 125-129 35.9349 37.0 37.0 37.0 37.0 37.0 130-134 35.908699999999996 37.0 37.0 37.0 37.0 37.0 135-139 35.845 37.0 37.0 37.0 37.0 37.0 140-144 35.8162 37.0 37.0 37.0 37.0 37.0 145-149 35.5932 37.0 37.0 37.0 37.0 37.0 150-151 35.58325 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 0.0 23 1.0 24 2.0 25 5.0 26 6.0 27 8.0 28 17.0 29 24.0 30 22.0 31 29.0 32 60.0 33 83.0 34 119.0 35 302.0 36 2947.0 37 374.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.25 11.200000000000001 8.774999999999999 41.775 2 20.30112923462986 14.353826850690087 35.60853199498118 29.73651191969887 3 19.400000000000002 16.900000000000002 27.075 36.625 4 23.35 26.275 23.5 26.875 5 23.0 30.4 25.674999999999997 20.925 6 19.1 34.9 25.3 20.7 7 15.5 26.325 43.15 15.024999999999999 8 17.025000000000002 26.35 31.05 25.575 9 18.45 24.65 35.25 21.65 10-14 19.865 29.15 28.12 22.865 15-19 19.55 27.295 28.575 24.58 20-24 19.455 28.804999999999996 27.725 24.015 25-29 19.77 28.53 27.88 23.82 30-34 19.759999999999998 28.705000000000002 28.07 23.465 35-39 20.39 28.865000000000002 27.715 23.03 40-44 20.555 28.544999999999998 27.495000000000005 23.405 45-49 19.575 28.22 28.384999999999998 23.82 50-54 19.775000000000002 28.689999999999998 27.834999999999997 23.7 55-59 20.635 28.15 27.505000000000003 23.71 60-64 20.375 27.765 28.449999999999996 23.41 65-69 20.57 28.83 27.265 23.335 70-74 19.900000000000002 28.63 27.925 23.544999999999998 75-79 20.330000000000002 28.575 27.355 23.74 80-84 20.535 29.160000000000004 27.88 22.425 85-89 21.29 28.655 27.04 23.015 90-94 20.36 29.825000000000003 27.08 22.735 95-99 20.919999999999998 28.52 27.450000000000003 23.11 100-104 20.555 28.565 27.83 23.05 105-109 20.155 28.610000000000003 27.88 23.355 110-114 20.979999999999997 28.825 27.235 22.96 115-119 21.085 28.96 27.029999999999998 22.925 120-124 20.330000000000002 28.939999999999998 27.785 22.945 125-129 21.065 29.544999999999998 26.515 22.875 130-134 20.315 28.15 28.005000000000003 23.53 135-139 20.625 27.805000000000003 28.23 23.34 140-144 20.405 28.345 27.425 23.825 145-149 20.595 28.799999999999997 26.96 23.645 150-151 19.7 28.9125 27.487499999999997 23.9 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.5 10 1.5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 1.0 18 1.0 19 0.5 20 0.5 21 0.0 22 1.5 23 1.5 24 3.5 25 5.0 26 4.0 27 7.5 28 9.0 29 11.0 30 22.5 31 29.0 32 30.5 33 51.5 34 60.5 35 66.0 36 92.0 37 98.0 38 135.5 39 185.5 40 182.5 41 194.5 42 233.0 43 251.5 44 271.5 45 286.5 46 257.0 47 226.0 48 232.0 49 218.5 50 168.5 51 143.5 52 120.5 53 90.5 54 75.5 55 54.0 56 45.5 57 37.0 58 30.0 59 25.5 60 13.0 61 11.0 62 8.5 63 3.0 64 0.0 65 0.0 66 0.5 67 0.5 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.375 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.14999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 65.90023382696805 42.275 2 21.161340607950116 27.150000000000002 3 7.40452065471551 14.249999999999998 4 3.6632891660171474 9.4 5 1.1301636788776306 3.6249999999999996 6 0.3897116134060795 1.5 7 0.19485580670303976 0.8750000000000001 8 0.03897116134060795 0.2 9 0.0779423226812159 0.44999999999999996 >10 0.03897116134060795 0.27499999999999997 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCGCTATATGGACATAGAGATTCATAGTACAGTGCCAGGAAGAGTTTATC 11 0.27499999999999997 No Hit GCCGAAGCTTATATGCTCCACAACATCTTGAAAAAAAATGTATCAATAAA 9 0.22499999999999998 No Hit CCGGGAACAATCTTTTGCTCAACAAGAACATCAACCATCTTCTTGCCATC 9 0.22499999999999998 No Hit ACCATCTTGACATACTTATATAGTACTTGTTTGTTCTTCTGCAGCCTTTA 8 0.2 No Hit CAGTTCTTGACCTTGCATCGGCCCAAAAAACATTAGCCATGCGATTTTCA 7 0.17500000000000002 No Hit GTTGATATGGTGCCCAAATGGGAGGTCGCTTCGGTCACAGCCTCTGTCCA 7 0.17500000000000002 No Hit CCCCATCTGTCCTTGTCGGTCTGCTTCTTCTCATCGTACTCTGCAACAAC 7 0.17500000000000002 No Hit CTTTTTTTTTCATGTGAATTTGTGTGTCCGGGTGGGGTGGGGTTGGTCAA 7 0.17500000000000002 No Hit GCCGTTGGGAATGCTCACAACAGTACGCCATTTGGCAAAACGAGCTCCCT 7 0.17500000000000002 No Hit CAGACATCATAATTCTTCCAGCTTTATTCCTCTTCCCCCTGACAACTCTC 6 0.15 No Hit CTTGCAACTGTTGATGACCCAATATCGACTCCAAGCTCCAGGTAGTTCTT 6 0.15 No Hit CCTTGCAGATATTGCTTAGAAGACTAAGCCAACGCTTTGAAACACATGCA 6 0.15 No Hit GGGTGTTCGAGGAGTTCCTGGTGGTGGAGGCTGTTGGGAGAGCAAGTGCC 6 0.15 No Hit CTCGTGGCCAGTTGGCACAAAGGTTCGCGAGCGATACTTTCTTGTTCCCT 6 0.15 No Hit CCCCCATTGGCGAATAGCGTCCTTGGATAGTTGCTAATCAACGTGCACCA 6 0.15 No Hit GGGGTTGAGCTTCACCGCCTTTCCGGCTAGCGAAGGGGAGGAGAGGGCCA 6 0.15 No Hit CTTCTCTACTTCTGCTAGCTACCTTGGCTTTGGTAAATACTATCTCTCCT 6 0.15 No Hit GTAAAGATTTAGTACAGTTCATAGTCCATCACAAATTGCTGCGACATGGT 6 0.15 No Hit GCCTAGCTTAGCGGAACTGCGTACGAGAAGCAAAAACAGCTCTATACTAT 6 0.15 No Hit CACGAGGATCATACTCTCCAAACCCATCCACCAATGAACATAGGTTAGAG 5 0.125 No Hit CGTCAAGCGTGATAAAATGTTATGAGCCAATACTTCATCAGACAACTGAA 5 0.125 No Hit GGGCTCTGCTGTTCTTGGATCTCTGCAGCTAACCGAGAGTCCTGAGGAGA 5 0.125 No Hit ACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGC 5 0.125 No Hit TAGTTAATTAGTCTATGAAGATCATCATAGACTGGATTCGACGACACTGA 5 0.125 No Hit GGACCATCTTCTCTACCTCTTCCTCTTCCACGCCCTACTCCAGGTGGCTT 5 0.125 No Hit GTCTACAAATCCAGGGAAAATCACCACATCCCTTTCTTCCTTGCCCTCCA 5 0.125 No Hit GCTAAATTTTCAAGAGATCCTGCCAACGATTCATCCATATGATCACCCAT 5 0.125 No Hit GGGGGATTTTGGATAGCAAGCAGAGTTCATTATTATGTGATTTACAGAGG 5 0.125 No Hit GTCGTAACACGTCGTCCCGTTTGGTGAGAAGGAGTTCAACGGTGTCGTCT 5 0.125 No Hit CCTCACTGCCTTCCAGGTCTCGATCATTCAAGTACAGAAAGCTTTTCTGA 5 0.125 No Hit CCGGGCTCTGTTGCTCAATGTTAACCAATACTTTGCACTTGTCAGCATCA 5 0.125 No Hit GTTGCCCTTGCTAATCAAGCAGGTTCCTTCATTAACGTTGCAGACTGGGT 5 0.125 No Hit CACTAAAGAAATGAATGTTGCTTCTTAACTGATCAATTGCTTCTAGGTAA 5 0.125 No Hit TGCATTGCACGTGCAGCAGCATCCCTACGAGTTCTACCCGATCCAGAAGC 5 0.125 No Hit CTAGGATTAGGCACTTCTAGATAAGGGTTCCTAGGCACTTCTAGATAAGG 5 0.125 No Hit GCCAACACCAAAGCCAATGTTTCTCCTGGTAAAGAGATTTGACACCTGGT 5 0.125 No Hit CATCATAGTACCCTGGTGAGCTGTGGTGCTCACGGTAGACCCAACCTTTC 5 0.125 No Hit CCTATTTGTATCTTTATTATGTTTTACATCAGTATTTTGTAGCTTGATAT 5 0.125 No Hit GCTGAGTTTGATTTCCTTCCAACTGCCAGTCCTGGAGATTTGGCAAGTGA 5 0.125 No Hit CTTGATAATACGAGCGTGAGATTTGTTGAAGACCCCACCCTAACGTGGTC 5 0.125 No Hit ATGCTCATCCTCCCAGGTTTTAGAATGCCAACAAGAGCTCCAGCCACAGA 5 0.125 No Hit GCAGCTTTAATAGCCTTCTGTAACTCACTTTCCAAGTTAGTCACCTTATT 5 0.125 No Hit GCTTCCTTGTAATTTCTAGTGCCTCAGGTGGCATAGTAGCGGAGAAAACC 5 0.125 No Hit CTATATTATCCACAAGTCCCCTCATTTGTTGCTCTCTTTCAGCCTTGACA 5 0.125 No Hit GCTTCCAAAGCTTCTATCCCGAGATGATGGCCTGCTTGATCTTCTACCAC 5 0.125 No Hit AGGCTCACCAAGCTCTGAGCTGGTTTCACTCCTCTCCTCATCATCATCAT 5 0.125 No Hit CCTGACTACGCTGCTGATCCACAGAGAAAAGCTGCATATTCCCTTTGACT 5 0.125 No Hit AACATCTTCAACCCTATACCAACCCAGCAAGCTTAAAATGCTTCCTGATC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.125 0.0 0.0 0.0 0.0 82-83 0.15 0.0 0.0 0.0 0.0 84-85 0.225 0.0 0.0 0.0 0.0 86-87 0.275 0.0 0.0 0.0 0.0 88-89 0.35 0.0 0.0 0.0 0.0 90-91 0.4125 0.0 0.0 0.0 0.0 92-93 0.4375 0.0 0.0 0.0 0.0 94-95 0.525 0.0 0.0 0.0 0.0 96-97 0.6125 0.0 0.0 0.0 0.0 98-99 0.7125 0.0 0.0 0.0 0.0 100-101 0.8875 0.0 0.0 0.0 0.0 102-103 1.0875 0.0 0.0 0.0 0.0 104-105 1.275 0.0 0.0 0.0 0.0 106-107 1.4 0.0 0.0 0.0 0.0 108-109 1.55 0.0 0.0 0.0 0.0 110-111 1.85 0.0 0.0 0.0 0.0 112-113 2.2249999999999996 0.0 0.0 0.0 0.0 114-115 2.6875 0.0 0.0 0.0 0.0 116-117 3.1500000000000004 0.0 0.0 0.0 0.0 118-119 3.5375 0.0 0.0 0.0 0.0 120-121 3.875 0.0 0.0 0.0 0.0 122-123 4.0625 0.0 0.0 0.0 0.0 124-125 4.4625 0.0 0.0 0.0 0.0 126-127 4.8875 0.0 0.0 0.0 0.0 128-129 5.2625 0.0 0.0 0.0 0.0 130-131 5.7875 0.0 0.0 0.0 0.0 132-133 6.1 0.0 0.0 0.0 0.0 134-135 6.45 0.0 0.0 0.0 0.0 136-137 7.0125 0.0 0.0 0.0 0.0 138-139 7.5625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTAGATT 10 0.006830828 145.0 145 CGCTGCT 10 0.006830828 145.0 9 ACGCTGC 10 0.006830828 145.0 8 AATTCTG 10 0.006830828 145.0 5 CAATTCT 10 0.006830828 145.0 4 CTGACTA 10 0.006830828 145.0 2 TACGCTG 10 0.006830828 145.0 7 CTACGCT 10 0.006830828 145.0 6 ACTACGC 10 0.006830828 145.0 5 >>END_MODULE SRR13695431 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695431_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.1495 37.0 37.0 37.0 37.0 37.0 2 36.1075 37.0 37.0 37.0 37.0 37.0 3 36.1495 37.0 37.0 37.0 37.0 37.0 4 36.21 37.0 37.0 37.0 37.0 37.0 5 36.279 37.0 37.0 37.0 37.0 37.0 6 36.3885 37.0 37.0 37.0 37.0 37.0 7 36.3005 37.0 37.0 37.0 37.0 37.0 8 36.2985 37.0 37.0 37.0 37.0 37.0 9 36.308 37.0 37.0 37.0 37.0 37.0 10-14 36.271100000000004 37.0 37.0 37.0 37.0 37.0 15-19 36.2479 37.0 37.0 37.0 37.0 37.0 20-24 36.2256 37.0 37.0 37.0 37.0 37.0 25-29 36.198499999999996 37.0 37.0 37.0 37.0 37.0 30-34 36.128750000000004 37.0 37.0 37.0 37.0 37.0 35-39 36.1492 37.0 37.0 37.0 37.0 37.0 40-44 36.1423 37.0 37.0 37.0 37.0 37.0 45-49 36.1219 37.0 37.0 37.0 37.0 37.0 50-54 36.06230000000001 37.0 37.0 37.0 37.0 37.0 55-59 36.084799999999994 37.0 37.0 37.0 37.0 37.0 60-64 36.0843 37.0 37.0 37.0 37.0 37.0 65-69 35.952600000000004 37.0 37.0 37.0 37.0 37.0 70-74 35.901300000000006 37.0 37.0 37.0 37.0 37.0 75-79 35.9818 37.0 37.0 37.0 37.0 37.0 80-84 35.957899999999995 37.0 37.0 37.0 37.0 37.0 85-89 35.8476 37.0 37.0 37.0 37.0 37.0 90-94 35.8608 37.0 37.0 37.0 37.0 37.0 95-99 35.880700000000004 37.0 37.0 37.0 37.0 37.0 100-104 35.789100000000005 37.0 37.0 37.0 37.0 37.0 105-109 35.7664 37.0 37.0 37.0 37.0 37.0 110-114 35.7024 37.0 37.0 37.0 37.0 37.0 115-119 35.724000000000004 37.0 37.0 37.0 37.0 37.0 120-124 35.5971 37.0 37.0 37.0 37.0 37.0 125-129 35.6364 37.0 37.0 37.0 37.0 37.0 130-134 35.4691 37.0 37.0 37.0 37.0 37.0 135-139 35.532450000000004 37.0 37.0 37.0 37.0 37.0 140-144 35.3524 37.0 37.0 37.0 32.2 37.0 145-149 35.196749999999994 37.0 37.0 37.0 29.8 37.0 150-151 34.99875 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 0.0 15 2.0 16 2.0 17 0.0 18 1.0 19 0.0 20 0.0 21 3.0 22 3.0 23 3.0 24 4.0 25 8.0 26 6.0 27 13.0 28 17.0 29 18.0 30 26.0 31 42.0 32 52.0 33 98.0 34 241.0 35 620.0 36 2623.0 37 217.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.70240480961924 20.06513026052104 12.424849699398797 28.80761523046092 2 28.15 24.925 32.074999999999996 14.85 3 20.9 29.375 30.475 19.25 4 24.0 33.35 23.175 19.475 5 23.474999999999998 37.65 21.625 17.25 6 20.349999999999998 39.2 23.375 17.075000000000003 7 20.200000000000003 20.849999999999998 40.300000000000004 18.65 8 19.825 27.35 30.575000000000003 22.25 9 21.45 25.25 31.1 22.2 10-14 23.155 30.409999999999997 26.715 19.72 15-19 22.425 28.325 28.315 20.935000000000002 20-24 22.52725272527253 29.322932293229325 27.93779377937794 20.212021202120212 25-29 23.43671835917959 28.4392196098049 28.01400700350175 20.110055027513756 30-34 21.550387596899228 28.797199299824953 28.327081770442607 21.32533133283321 35-39 21.52215221522152 29.127912791279126 27.742774277427745 21.607160716071608 40-44 22.291687506251876 28.623587076122835 28.02840852255677 21.05631689506852 45-49 22.379475895179034 28.490698139627924 28.190638127625522 20.939187837567513 50-54 22.367236723672367 28.5028502850285 27.502750275027505 21.627162716271627 55-59 21.68584292146073 28.4392196098049 28.289144572286144 21.585792896448226 60-64 21.807180718071805 27.57275727572757 29.347934793479347 21.272127212721273 65-69 22.264999999999997 28.249999999999996 28.499999999999996 20.985 70-74 22.821410705352676 27.983991995997997 27.658829414707352 21.53576788394197 75-79 22.511753526057817 28.19845953786136 28.133440032009606 21.15634690407122 80-84 23.47 27.584999999999997 27.779999999999998 21.165 85-89 23.42436974789916 27.96618647458984 27.841136454581832 20.76830732292917 90-94 22.24222422242224 28.937893789378936 28.05780578057806 20.762076207620762 95-99 22.21 28.49 28.904999999999998 20.395 100-104 23.56178089044522 27.83391695847924 28.019009504752372 20.58529264632316 105-109 23.516758379189596 28.544272136068034 27.773886943471737 20.165082541270635 110-114 23.362336233623363 28.457845784578456 28.072807280728075 20.10701070107011 115-119 23.631815907953975 27.838919459729865 28.044022011005502 20.485242621310658 120-124 24.027013506753377 27.178589294647328 27.763881940970485 21.030515257628814 125-129 23.82953181272509 29.061624649859947 26.66066426570628 20.448179271708682 130-134 24.189513708224936 26.46087652591555 28.692215329197516 20.657394436661995 135-139 24.393416379008453 27.880334183801093 27.34503977187453 20.381209665315925 140-144 24.927492749274926 27.742774277427745 27.482748274827486 19.846984698469846 145-149 25.47401070588824 27.905347941367754 27.735254389914456 18.885386962829557 150-151 24.78108581436077 27.408056042031525 27.23292469352014 20.577933450087567 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.0 16 0.5 17 3.0 18 2.5 19 0.0 20 1.0 21 2.5 22 3.5 23 2.5 24 2.0 25 4.0 26 5.5 27 10.5 28 15.0 29 17.0 30 23.5 31 29.0 32 41.0 33 46.0 34 51.0 35 66.5 36 99.5 37 124.5 38 138.5 39 182.5 40 242.5 41 240.0 42 247.0 43 281.5 44 265.0 45 255.5 46 260.0 47 243.5 48 203.5 49 172.0 50 154.0 51 125.5 52 99.5 53 87.0 54 55.5 55 46.5 56 47.5 57 28.5 58 16.5 59 10.5 60 8.0 61 9.0 62 8.0 63 5.5 64 2.5 65 1.0 66 3.0 67 3.0 68 1.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 1.0 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.01 25-29 0.05 30-34 0.025 35-39 0.01 40-44 0.03 45-49 0.02 50-54 0.01 55-59 0.05 60-64 0.01 65-69 0.0 70-74 0.05 75-79 0.03 80-84 0.0 85-89 0.04 90-94 0.01 95-99 0.0 100-104 0.05 105-109 0.05 110-114 0.01 115-119 0.05 120-124 0.05 125-129 0.04 130-134 0.06 135-139 0.055 140-144 0.01 145-149 0.055 150-151 0.075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.55 #Duplication Level Percentage of deduplicated Percentage of total 1 66.92486444616577 43.2 2 20.3718048024787 26.3 3 7.242447714949652 14.025000000000002 4 3.601859024012394 9.3 5 1.1231603408210689 3.6249999999999996 6 0.34856700232378 1.35 7 0.1549186676994578 0.7000000000000001 8 0.11618900077459333 0.6 9 0.03872966692486445 0.22499999999999998 >10 0.0774593338497289 0.675 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGAAAACGTCTCGCCTCAATCGGGCTAGAGAACACCGAGGCTAACCGCCA 16 0.4 No Hit ATCAAAAGCTTTCAGTGATGGTTTCTCGTCTAACAACAACCCTCTCTTTC 11 0.27499999999999997 No Hit CTTCAATCGTAAATCACAAATACATACACGTTTACTCATCAGCTCGAAAA 9 0.22499999999999998 No Hit TGATGGTTCATAAGAACAATACTGATGTAAAAAGCAATATGGATTTGGCT 8 0.2 No Hit ATTGCATACATCGTTACTTAATTTAGTTGGGGTTGTCCACTCGTTGGATA 8 0.2 No Hit GGAACAATAAGAAAGGTCACTTTTGTTGAAGGCAAAGGACTCACCTACGT 8 0.2 No Hit GGAGCACTGCATAGCTTATAAGCTTGTAAGAGATGGCTTCCTCCTCTATG 7 0.17500000000000002 No Hit TGAAATTTTAAGCCTTGAATCCTTGGGTTCTGGTTCTTGGCCAATGCCTC 7 0.17500000000000002 No Hit GTTGTTTCTAGTCGTGTTGGCCAAGGAGTGGGAGTTGTTCCAAGTGGTGT 7 0.17500000000000002 No Hit AAACTCTGCATTTGCTAGCTAGAAGTCACTCTACCCTTCGCATTACTTCC 7 0.17500000000000002 No Hit AAAGAAACAACATGTCGTCGACGACAAAACCAAAGGCAGTGAAGCACACT 6 0.15 No Hit GTTTGATTCCGAGACACTGCAGAGATGTGCAAAACTGAAATCTAAGGAGG 6 0.15 No Hit CTTCTGTCTGAGAATGCAATCACCATGTCTTCACCTCTGCAAGGTTTTCA 6 0.15 No Hit TGCAAGTTTAAGATTTTCTATTCTTTTTAGAGAAAGAAGGAAGTTTTCTT 6 0.15 No Hit AGGTAGCGGGGTTATAAATTGGTTTTACACCCACGAAGCTGTCTGGTCTT 6 0.15 No Hit ATCAAGAGTGTCTGCCTCTCACTGCTCCAACGTTTCCACTTCAAATTAGT 6 0.15 No Hit CAAGATTTAAACTCATACCATATATATCTAAGGGTTCCTGGATAGTTAAG 6 0.15 No Hit ACAAACCATAAGGAGCAGAACCTCTTTTTGTCTATCGGCCGTCCTGTGGA 6 0.15 No Hit GCTTATAGCTGCTGTTGGTCATCTTTATGCATTTCCATACAAAGAGTATG 6 0.15 No Hit GGGTAATAATCGTGGACAAATGTAGTGTGCTTGACTTAACCTGACAAATT 5 0.125 No Hit TCTGGGTGTCAAGGTTCATGCTTGTGTTGGTGGAACTAGTGTTCGTGAGG 5 0.125 No Hit ATTCACTTGGTGAAAGAGCAGCAAAGATGAAAGAAGCACTTCAGAAAAGC 5 0.125 No Hit CATGCTTCCTCTTTCTTTACTCAAGACTGCTCAAGGCCACCCAATGTTGG 5 0.125 No Hit CAACTAGATTGCCCATCCTTTGAAAGTAGATATGCTACCCACTGGGTTGA 5 0.125 No Hit GGAAGAGAAAGGAAGACATGCCTTCTTTATCTCTTACTAGCAACAACAGC 5 0.125 No Hit GGGGGACTCAATCTGACAAGAAACACCAGAGTCATGTTACGGTGAAAGGA 5 0.125 No Hit TTTTGCCAGAGAGGCAACCGGTCTGGAAAGATGGAAAGAAAGAAGGGTTT 5 0.125 No Hit GCCCAAGCAGCTGAAGCCGCCCAAGCTGTCCAGGTCAGAGCCTGTGCATG 5 0.125 No Hit GTTTATCATTGGTCAATTGAAGGTGATTCGGAGCCTGTGAAGATGTTTGA 5 0.125 No Hit CCCTTATCACCACATCTCTTACTTCCCTCCCAAAACCCAAAAAACCCTTA 5 0.125 No Hit TGGAAAGATTAGCCCAAATTGAGAGGGCTCAGAGGCATATTGAAAGTTTG 5 0.125 No Hit CACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATC 5 0.125 No Hit GGCTTAAATAGTAGAGCGTTGCATTAAATTAAATCACACTCTCTATCTTC 5 0.125 No Hit GTCTGATTAGAAACTCTCACACAGGCCGACAAACTCTTAAGCTTTCCTTT 5 0.125 No Hit ACTTAATTTCGATTTCCACATAAAAAAAAAACAACACTTTAAAGAAAAAC 5 0.125 No Hit GCAAAAGAATTGTTGAATAAGCAACTACCACCTGGAAACACTATTGAAAA 5 0.125 No Hit CTTCGGTTTTCAAGCTCTATACAAGGTAGTCATTCTAAATTCAAAGAGCA 5 0.125 No Hit TGCAAAGACTTGCAACGGAGAACCAGAGACTTGCAGCAACTCATGGAACC 5 0.125 No Hit CAGGCTCAGACTTGTACCTATACATGACGATGATGGAGCTATCACACACA 5 0.125 No Hit ATGTTTCTCTGACAGCAGCCCGCTGTTGCCTCCAGATGAAGCAAAGGCTT 5 0.125 No Hit TGCTAGTGAGATCTTTCCTGTTCCTGTAGTGAAAGCTCTTCTCTACCCTG 5 0.125 No Hit CTCCACCTTCGGAACCTGGAGAATAGCGAAACTGCTGCTACCAATACAGT 5 0.125 No Hit GATCTTTCAAGTGGAACAAAGGCTCCTTCTTCACCAGCAAGGATTCAAGC 5 0.125 No Hit TGAGACGGCATTGAAAAAGGCAGTGGCAAATCAGCCAGTGAGTGTTGCAA 5 0.125 No Hit GGAAAAAATTGTTCATGGGAGTGGTTTTCATGAAATTATTGATAAAAAAC 5 0.125 No Hit GAAATTGCCTCTCTGCTAGCAGAAGGAAAGGTCCCAATTGGTGTTGGTCA 5 0.125 No Hit CTGATGCCTGGTATCTTCACAGGATGTGCCTCATGCATGTCTGCTGATTT 5 0.125 No Hit AGTGGATGCCATGGGATGATTATGTAGCGCAGCCATTCGTCCAAAAGCAT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.125 0.0 0.0 0.0 0.0 82-83 0.15 0.0 0.0 0.0 0.0 84-85 0.225 0.0 0.0 0.0 0.0 86-87 0.275 0.0 0.0 0.0 0.0 88-89 0.35 0.0 0.0 0.0 0.0 90-91 0.4125 0.0 0.0 0.0 0.0 92-93 0.4375 0.0 0.0 0.0 0.0 94-95 0.525 0.0 0.0 0.0 0.0 96-97 0.6125 0.0 0.0 0.0 0.0 98-99 0.7125 0.0 0.0 0.0 0.0 100-101 0.8875 0.0 0.0 0.0 0.0 102-103 1.0875 0.0 0.0 0.0 0.0 104-105 1.275 0.0 0.0 0.0 0.0 106-107 1.4 0.0 0.0 0.0 0.0 108-109 1.55 0.0 0.0 0.0 0.0 110-111 1.85 0.0 0.0 0.0 0.0 112-113 2.25 0.0 0.0 0.0 0.0 114-115 2.7125000000000004 0.0 0.0 0.0 0.0 116-117 3.1875 0.0 0.0 0.0 0.0 118-119 3.6125 0.0 0.0 0.0 0.0 120-121 3.95 0.0 0.0 0.0 0.0 122-123 4.137499999999999 0.0 0.0 0.0 0.0 124-125 4.5375 0.0 0.0 0.0 0.0 126-127 4.9625 0.0 0.0 0.0 0.0 128-129 5.3375 0.0 0.0 0.0 0.0 130-131 5.9125 0.0 0.0 0.0 0.0 132-133 6.2125 0.0 0.0 0.0 0.0 134-135 6.55 0.0 0.0 0.0 0.0 136-137 7.1625 0.0 0.0 0.0 0.0 138-139 7.725 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTGGTC 10 0.006830828 145.0 8 TTATCAT 10 0.006830828 145.0 3 TATCATT 10 0.006830828 145.0 4 GAATTGA 10 0.006830828 145.0 4 ATCATTG 10 0.006830828 145.0 5 CTTAAAG 10 0.006830828 145.0 145 TTGGTCA 10 0.006830828 145.0 9 TTTATCA 10 0.006830828 145.0 2 TTTTTTT 85 2.0147776E-4 13.6470585 10-14 >>END_MODULE Read 1109833 spots for SRR13695431.sra Written 1109833 spots for SRR13695431.sra Read 1109833 spots for SRR13695431.sra Written 1109833 spots for SRR13695431.sra Read 1109833 spots for SRR13695431.sra Written 1109833 spots for SRR13695431.sra Read 1109833 spots for SRR13695431.sra Written 1109833 spots for SRR13695431.sra Read 1109833 spots for SRR13695431.sra Written 1109833 spots for SRR13695431.sra Read 1109833 spots for SRR13695431.sra Written 1109833 spots for SRR13695431.sra Read 1109833 spots for SRR13695431.sra Written 1109833 spots for SRR13695431.sra Read 1109833 spots for SRR13695431.sra Written 1109833 spots for SRR13695431.sra Read 1109833 spots for SRR13695431.sra Written 1109833 spots for SRR13695431.sra Read 1109833 spots for SRR13695431.sra Written 1109833 spots for SRR13695431.sra Read 1109833 spots for SRR13695431.sra Written 1109833 spots for SRR13695431.sra Read 1109833 spots for SRR13695431.sra Written 1109833 spots for SRR13695431.sra Read 1109833 spots for SRR13695431.sra Written 1109833 spots for SRR13695431.sra Read 1109833 spots for SRR13695431.sra Written 1109833 spots for SRR13695431.sra Read 1109833 spots for SRR13695431.sra Written 1109833 spots for SRR13695431.sra Read 1109851 spots for SRR13695431.sra Written 1109851 spots for SRR13695431.sra Read 1109833 spots for SRR13695431.sra Written 1109833 spots for SRR13695431.sra Read 1109833 spots for SRR13695431.sra Written 1109833 spots for SRR13695431.sra Read 1109833 spots for SRR13695431.sra Written 1109833 spots for SRR13695431.sra Read 1109833 spots for SRR13695431.sra Written 1109833 spots for SRR13695431.sra SRR ids: ['SRR13695431.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_33jdtphq SRR13695431.sra spots: 22196678 blocks: [[1, 1109833], [1109834, 2219666], [2219667, 3329499], [3329500, 4439332], [4439333, 5549165], [5549166, 6658998], [6658999, 7768831], [7768832, 8878664], [8878665, 9988497], [9988498, 11098330], [11098331, 12208163], [12208164, 13317996], [13317997, 14427829], [14427830, 15537662], [15537663, 16647495], [16647496, 17757328], [17757329, 18867161], [18867162, 19976994], [19976995, 21086827], [21086828, 22196678]] SRR13695431 file size 7521701 SRR13695431 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695431 SRR13695431_1.fastq SRR13695431_2.fastq Input file: SRR13695431_1.fastq Paired file: SRR13695431_2.fastq trimmed: SRR13695431-trimmed-pair1.fastq, SRR13695431-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 02:09:38 2025 >> started Wed Feb 12 02:10:03 2025 >> done (24.737s) 22196678 read pairs processed; of these: 154 ( 0.00%) short read pairs filtered out after trimming by size control 2269 ( 0.01%) empty read pairs filtered out after trimming by size control 22194255 (99.99%) read pairs available; of these: 2614488 (11.78%) trimmed read pairs available after processing 19579767 (88.22%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 4 0.00% 19 1 0.00% 20 4 0.00% 21 0 0.00% 22 0 0.00% 23 7 0.00% 24 0 0.00% 25 2 0.00% 26 4 0.00% 27 0 0.00% 28 2 0.00% 29 12 0.00% 30 7 0.00% 31 5 0.00% 32 10 0.00% 33 1 0.00% 34 10 0.00% 35 16 0.00% 36 12 0.00% 37 14 0.00% 38 13 0.00% 39 27 0.00% 40 22 0.00% 41 29 0.00% 42 38 0.00% 43 32 0.00% 44 33 0.00% 45 40 0.00% 46 39 0.00% 47 39 0.00% 48 55 0.00% 49 73 0.00% 50 121 0.00% 51 114 0.00% 52 150 0.00% 53 137 0.00% 54 143 0.00% 55 183 0.00% 56 215 0.00% 57 216 0.00% 58 249 0.00% 59 286 0.00% 60 335 0.00% 61 397 0.00% 62 514 0.00% 63 489 0.00% 64 598 0.00% 65 668 0.00% 66 741 0.00% 67 848 0.00% 68 995 0.00% 69 1132 0.01% 70 1269 0.01% 71 1447 0.01% 72 1691 0.01% 73 1908 0.01% 74 2098 0.01% 75 2379 0.01% 76 2590 0.01% 77 2869 0.01% 78 3088 0.01% 79 3739 0.02% 80 3913 0.02% 81 4574 0.02% 82 5074 0.02% 83 5625 0.03% 84 6500 0.03% 85 6859 0.03% 86 7420 0.03% 87 7842 0.04% 88 8805 0.04% 89 9099 0.04% 90 9752 0.04% 91 11215 0.05% 92 11612 0.05% 93 12656 0.06% 94 13620 0.06% 95 14430 0.07% 96 15388 0.07% 97 16487 0.07% 98 17131 0.08% 99 17920 0.08% 100 19198 0.09% 101 19949 0.09% 102 20960 0.09% 103 21995 0.10% 104 23355 0.11% 105 23867 0.11% 106 25691 0.12% 107 26301 0.12% 108 27416 0.12% 109 28595 0.13% 110 29093 0.13% 111 29917 0.13% 112 31274 0.14% 113 32591 0.15% 114 33715 0.15% 115 35394 0.16% 116 36752 0.17% 117 37865 0.17% 118 38992 0.18% 119 39716 0.18% 120 41021 0.18% 121 42720 0.19% 122 43033 0.19% 123 43851 0.20% 124 45288 0.20% 125 46906 0.21% 126 48443 0.22% 127 49357 0.22% 128 50305 0.23% 129 51351 0.23% 130 52209 0.24% 131 53445 0.24% 132 55028 0.25% 133 55747 0.25% 134 56767 0.26% 135 57922 0.26% 136 59175 0.27% 137 59714 0.27% 138 60951 0.27% 139 63571 0.29% 140 64132 0.29% 141 64476 0.29% 142 66126 0.30% 143 65944 0.30% 144 67092 0.30% 145 69343 0.31% 146 69592 0.31% 147 70818 0.32% 148 72172 0.33% 149 73563 0.33% 150 73708 0.33% 151 19579767 88.22% 22194255 reads passed initial QC criterion=sequence-density sequence-density=0.27 sequence-density-rank=1 fanout-score=2.57 fanout-score-rank=32 prefix-density=0.29 prefix-fanout=2.3 sequence=TGAGATGCCTCAGTGCATCCAAACATGGGTAG criterion=fanout-score sequence-density=0.01 sequence-density-rank=35 fanout-score=120.85 fanout-score-rank=1 prefix-density=0.14 prefix-fanout=8.5 sequence=TCTCCAAAAACCCAATAAAAAAGGAAGAGGTAAAGCATTTTGCCAAGGTCTAAGTACAATTTAAACAAACCACCCCTAAGGCCCTAACAGATATAGCTAGGGACACATCAAGTTCTGGGACGACTTAGACACCTTTCGGCTTGGCGGCAATAAAGCTGATGCACTGCACTTGACGCGTGTTGTCGAATCCGATTATACGGATAAAGGCGTTAGGGTAAGCTTTCTT criterion=sequence-density sequence-density=0.40 sequence-density-rank=1 fanout-score=2.06 fanout-score-rank=34 prefix-density=0.41 prefix-fanout=2.0 sequence=TGTAAGAGATGGCTTCCTC criterion=fanout-score sequence-density=0.14 sequence-density-rank=8 fanout-score=35.23 fanout-score-rank=1 prefix-density=0.40 prefix-fanout=12.7 sequence=AAAGAAAAGAAAA SRR13695431 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 02:10:42 Started mapping on | Feb 12 02:10:42 Finished on | Feb 12 02:13:11 Mapping speed, Million of reads per hour | 536.24 Number of input reads | 22194255 Average input read length | 296 UNIQUE READS: Uniquely mapped reads number | 20832862 Uniquely mapped reads % | 93.87% Average mapped length | 294.71 Number of splices: Total | 19936614 Number of splices: Annotated (sjdb) | 19469954 Number of splices: GT/AG | 19542082 Number of splices: GC/AG | 309070 Number of splices: AT/AC | 11586 Number of splices: Non-canonical | 73876 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 2.90 Insertion rate per base | 0.02% Insertion average length | 2.45 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 532445 % of reads mapped to multiple loci | 2.40% Number of reads mapped to too many loci | 49218 % of reads mapped to too many loci | 0.22% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.43% % of reads unmapped: other | 0.09% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 829206 829206 829206 N_multimapping 532445 532445 532445 N_noFeature 885992 20397837 1176888 N_ambiguous 279236 2021 133705 UnstrandedReadsAssigned:19667634 PositiveStrandReadsAssigned:433004 NegativeStrandReadsAssigned:19522269 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695431 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695431-trimmed-pair1.fastq SRR13695431-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 22,194,255 reads, 19,570,494 reads pseudoaligned [quant] estimated average fragment length: 248.505 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,135 rounds 52401 SRR13695431.ke.tsv 34699 SRR13695431.se.tsv 87100 total ==> SRR13695431.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1770.5 842 25.8458 Potri.005G024800.1.v4.1 1035 787.495 402 27.7428 Potri.004G059700.1.v4.1 961 713.672 14 1.06611 Potri.007G009000.2.v4.1 1416 1168.5 0 0 Potri.003G141000.2.v4.1 2943 2695.5 1031.39 20.7949 Potri.016G087400.1.v4.1 270 86.1782 917 578.288 Potri.015G069301.1.v4.1 564 326.441 0 0 Potri.010G195200.1.v4.1 1773 1525.5 135 4.80945 Potri.012G127500.1.v4.1 977 729.567 228 16.9841 ==> SRR13695431.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 743 Potri.001G233950.v4.1 2 Potri.001G122700.v4.1 319 Potri.001G212900.v4.1 1 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 28 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 3 SRR13695431 completed mapping pipeline successfully