Starting /dee2/code/volunteer_pipeline.sh SRR13695432 current disk space = 3049030082560 free memory = 1548766448 SRR13695432 SRAfilesize 6b99d6b3ca1432d5711665df0acd0d76 SRR13695432.sra SRR13695432.sra file validated SRR13695432 is paired end SRR13695432 is conventional basespace SRR13695432 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695432_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.645 37.0 37.0 37.0 37.0 37.0 2 36.447 37.0 37.0 37.0 37.0 37.0 3 36.601 37.0 37.0 37.0 37.0 37.0 4 36.616 37.0 37.0 37.0 37.0 37.0 5 36.611 37.0 37.0 37.0 37.0 37.0 6 36.6665 37.0 37.0 37.0 37.0 37.0 7 36.5175 37.0 37.0 37.0 37.0 37.0 8 36.5875 37.0 37.0 37.0 37.0 37.0 9 36.5835 37.0 37.0 37.0 37.0 37.0 10-14 36.5847 37.0 37.0 37.0 37.0 37.0 15-19 36.528099999999995 37.0 37.0 37.0 37.0 37.0 20-24 36.4589 37.0 37.0 37.0 37.0 37.0 25-29 36.4426 37.0 37.0 37.0 37.0 37.0 30-34 36.4745 37.0 37.0 37.0 37.0 37.0 35-39 36.424899999999994 37.0 37.0 37.0 37.0 37.0 40-44 36.424 37.0 37.0 37.0 37.0 37.0 45-49 36.3877 37.0 37.0 37.0 37.0 37.0 50-54 36.357499999999995 37.0 37.0 37.0 37.0 37.0 55-59 36.3988 37.0 37.0 37.0 37.0 37.0 60-64 36.3889 37.0 37.0 37.0 37.0 37.0 65-69 36.3251 37.0 37.0 37.0 37.0 37.0 70-74 36.3183 37.0 37.0 37.0 37.0 37.0 75-79 36.255900000000004 37.0 37.0 37.0 37.0 37.0 80-84 36.2156 37.0 37.0 37.0 37.0 37.0 85-89 36.244699999999995 37.0 37.0 37.0 37.0 37.0 90-94 36.202999999999996 37.0 37.0 37.0 37.0 37.0 95-99 36.13439999999999 37.0 37.0 37.0 37.0 37.0 100-104 36.1656 37.0 37.0 37.0 37.0 37.0 105-109 36.0992 37.0 37.0 37.0 37.0 37.0 110-114 36.097699999999996 37.0 37.0 37.0 37.0 37.0 115-119 36.076499999999996 37.0 37.0 37.0 37.0 37.0 120-124 35.971199999999996 37.0 37.0 37.0 37.0 37.0 125-129 35.9424 37.0 37.0 37.0 37.0 37.0 130-134 35.958800000000004 37.0 37.0 37.0 37.0 37.0 135-139 35.783699999999996 37.0 37.0 37.0 37.0 37.0 140-144 35.66799999999999 37.0 37.0 37.0 37.0 37.0 145-149 35.5381 37.0 37.0 37.0 37.0 37.0 150-151 35.331 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 1.0 21 1.0 22 1.0 23 0.0 24 1.0 25 4.0 26 4.0 27 8.0 28 15.0 29 14.0 30 21.0 31 41.0 32 41.0 33 71.0 34 142.0 35 341.0 36 2957.0 37 336.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.125 11.799999999999999 9.625 39.45 2 21.246246246246248 12.912912912912914 36.38638638638639 29.454454454454453 3 18.675 17.25 27.625 36.449999999999996 4 23.05 24.175 24.175 28.599999999999998 5 23.724999999999998 29.375 25.15 21.75 6 21.3 33.95 24.425 20.325 7 16.025 27.85 38.925 17.2 8 16.950000000000003 26.575 32.800000000000004 23.674999999999997 9 17.525 25.35 35.125 22.0 10-14 19.935 29.225 28.1 22.74 15-19 20.19 27.62 27.825 24.365000000000002 20-24 20.87 27.884999999999998 28.345 22.900000000000002 25-29 20.62 28.675 27.16 23.544999999999998 30-34 20.945 27.474999999999998 28.134999999999998 23.445 35-39 20.72 28.360000000000003 26.905 24.015 40-44 20.905 28.43 27.339999999999996 23.325000000000003 45-49 21.015 28.12 27.825 23.04 50-54 20.805 28.294999999999998 27.97 22.93 55-59 20.915 28.355000000000004 27.47 23.26 60-64 20.39 28.525 27.305 23.78 65-69 20.205000000000002 28.93 27.21 23.655 70-74 20.695 29.015 26.634999999999998 23.655 75-79 21.26 27.779999999999998 27.505000000000003 23.455000000000002 80-84 20.77 28.610000000000003 26.72 23.9 85-89 20.755000000000003 27.915 27.215 24.115000000000002 90-94 20.535 27.92 27.560000000000002 23.985 95-99 20.575 28.075 27.79 23.56 100-104 21.099999999999998 27.55 27.644999999999996 23.705000000000002 105-109 20.95 28.22 27.815 23.015 110-114 20.87 27.87 27.694999999999997 23.565 115-119 21.695 27.72 27.224999999999998 23.36 120-124 20.57 27.77 27.665 23.995 125-129 21.349999999999998 27.775 26.924999999999997 23.95 130-134 21.32 28.415000000000003 26.295 23.97 135-139 21.485000000000003 28.605000000000004 26.05 23.86 140-144 21.415 27.99 26.775 23.82 145-149 21.26 28.18 26.155 24.404999999999998 150-151 22.075 28.65 26.087500000000002 23.1875 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 0.0 17 0.5 18 1.0 19 1.0 20 1.5 21 1.5 22 2.0 23 3.5 24 6.5 25 5.5 26 3.5 27 4.5 28 12.5 29 13.5 30 13.0 31 23.0 32 36.5 33 43.5 34 48.0 35 73.0 36 92.0 37 88.5 38 125.0 39 168.0 40 179.5 41 193.0 42 202.0 43 245.5 44 260.5 45 231.5 46 227.0 47 248.0 48 247.0 49 223.5 50 191.0 51 139.0 52 124.5 53 109.5 54 80.5 55 76.0 56 66.5 57 47.0 58 33.0 59 31.0 60 23.5 61 13.0 62 12.0 63 9.0 64 5.5 65 4.0 66 2.5 67 1.5 68 2.0 69 1.0 70 0.5 71 0.5 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.1 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.77499999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 71.58724471515586 49.95 2 19.419562880687927 27.1 3 5.553565030455034 11.625 4 1.8989609458975278 5.3 5 1.0390541024722322 3.6249999999999996 6 0.2508061626657112 1.05 7 0.14331780723754925 0.7000000000000001 8 0.07165890361877462 0.4 9 0.0 0.0 >10 0.03582945180938731 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTCAGCATAGAGTGGCCACACGATTAATGGCACACCATTCACGACACTTT 10 0.25 No Hit GCTAGCAATTGGTCGATTGGGATTCTTGAGTCCAACACGAGCTCGGCGGA 8 0.2 No Hit GTCAAGATATTGTTGTGGACAGGATCGGCCAGGTGATCCAGGAGGTTTTG 8 0.2 No Hit CCAGCCCACCATGGCAAACTGATATAACTTGCTCCAAGGGCGAAATTCCC 7 0.17500000000000002 No Hit GTCATGGTAAGCTTCAACTTTAAGGCATTTCTCTCTTTTGTTTGTAGTGC 7 0.17500000000000002 No Hit GCTTCTTGTCAAATCCCCAAAATGAAGCGGCCTTCGCATCATCTTCAATC 7 0.17500000000000002 No Hit GGTCCTTGCACTTGGGTAGCAGTTGCCAGTTCCTCAGGAGCAGAATCACC 7 0.17500000000000002 No Hit CTAACTGCCATATCATTAGTAGGCACTGCCAAAGCAGCAGCACGCTTCTT 6 0.15 No Hit CTCTGCAATAGACTGGTCTTCCACCAGCAGCCTCAGCAGCGGCAGCTGCC 6 0.15 No Hit TATTATTATTCATCGACACAGACAAGATACATATATAGAGGGATGGCCGC 6 0.15 No Hit GTTGCGACTTCATTCAGAAACAGATGCAATGTAGGTGATCAAGTCAACCA 6 0.15 No Hit CTCACATCTGGGATTAGTGATCCTCAATCTTCTTCAGGCTTTTCATGTCT 6 0.15 No Hit GCACAGTTTGAACTTTTCCTCTGCAACAGCCTTTGAGGAGCCCTGGTGAC 6 0.15 No Hit TCAGCACCAAGCATTACTGTATCCTGTAAGTGGGCATTTGAGTTTATTCG 6 0.15 No Hit GCCCTCGTCGCAGTAGTCCCTTGATGGTCCATTGATCTCTTCTATGTAGC 5 0.125 No Hit CTTCTGTGCAACCAGGGAGGCCAAATCATCTTTATCTGTTTTGCGCCACC 5 0.125 No Hit TTACAGAACATGCACCAAATTTCTCAAACTTTTGAAGGTGAACACAAAAA 5 0.125 No Hit ATTGGATTTCTTTGTTTTGGACCAAGGCTGAGCTTGAAGTTCCTCAATCC 5 0.125 No Hit CTCAGTGAACTGTTTGACTCCCTTATTACTGCATTTGTCACTAATACCAT 5 0.125 No Hit CTGATGGATAAACCTTATGGTGGCAAAACAAATAATGAAACCTTGCTCGG 5 0.125 No Hit CAGGTAAATGACAATCTGCACACACTCCAGGCTGGCAGCAACAAAGACAT 5 0.125 No Hit ATCACCTTCAACCCTTGAGAAAGTGCATATGCAACCTTGTCGCCCACAAA 5 0.125 No Hit TGATATCTTCACGAATTGCAGCATGTGGGCAGCCTCCAGTTTCAACAGCA 5 0.125 No Hit GTTGTCACACATGCAGTGCTGCCTTACTATTTAAGCAAACACAGTGTTGT 5 0.125 No Hit CCTCCTTCAAAAACTCCCTCTTTCTCAAACTCCAAACTAAACTCTGTTCA 5 0.125 No Hit GGGGAGAATTGATAAGTTTCTCAGATTTCCAATAGAAGAAGGGATCCTAC 5 0.125 No Hit GCAGCCATTCTCAGCACCAAAGTTCATCTCAGAGCTCTCGTAGAACATCC 5 0.125 No Hit CCACGCTAGGCTGAGGGGGCTCGTAAGAATCAAGAAGCATCGGCAGATAC 5 0.125 No Hit CATGCGTCGATGCTCTGTCTGCTTATCTTGCTTACGAGAAAGTCCTTCTC 5 0.125 No Hit TCTCCCTCACTGTCAATCCTCGGCATTGCTTTCATTTTGCTGCTTGGATG 5 0.125 No Hit CCTGTATATCTTTCATGGGTACTCCAGGCCTAGCTACGAGGGAAGTTTCT 5 0.125 No Hit CAGTTGTAGGATCAGATGTGAAGCAAACCAGATCAACCCCATACTTTTTG 5 0.125 No Hit AAGAAATCTTGCTCGGCAAAGGGGGTTGGTGGGGTGATCTGCAGCCTCTC 5 0.125 No Hit GTAGGCCATTGCTCCAGGGATTAAAGATATCATGAGCAGGTAAGGAATGG 5 0.125 No Hit TTTAGACAAGAAGTTTGATTTTGTAATTTGATTTATGTACTAGGAAGGTG 5 0.125 No Hit GTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACC 5 0.125 No Hit GCTGCCAACAAAGTCTGCAAAAGTTCCAGATACAACCCTAACAGTTGAAC 5 0.125 No Hit CAACGATCTTTTTGCCAGAGCCCAGGTACAATTTGAACAAAGCAACCCTA 5 0.125 No Hit GTTTTTTTTCCCCGACCAATATTCTAAATGCATTTCTAGCACTCAATGCA 5 0.125 No Hit CCCACGGCCAGAAGATAAATCTTCTCTCTCTGTAGCAAAGGGGGGCTACT 5 0.125 No Hit CATTTGACTTGAAAGATCGGATTTTGGACACCACATGAGAACTAAACAAT 5 0.125 No Hit CTCATTCAATATACCCATCTCTGATCCAAAAACACTTAATCTGATCTAAA 5 0.125 No Hit CTGGATGTTGTAGTCGCTCAGGGTGCGACCATCCTCGAGCTGTTTGCCGG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.037500000000000006 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.0625 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.15 0.0 0.0 0.0 0.0 78-79 0.175 0.0 0.0 0.0 0.0 80-81 0.2125 0.0 0.0 0.0 0.0 82-83 0.275 0.0 0.0 0.0 0.0 84-85 0.35 0.0 0.0 0.0 0.0 86-87 0.425 0.0 0.0 0.0 0.0 88-89 0.55 0.0 0.0 0.0 0.0 90-91 0.6625 0.0 0.0 0.0 0.0 92-93 0.7124999999999999 0.0 0.0 0.0 0.0 94-95 0.825 0.0 0.0 0.0 0.0 96-97 0.9624999999999999 0.0 0.0 0.0 0.0 98-99 1.3250000000000002 0.0 0.0 0.0 0.0 100-101 1.5125000000000002 0.0 0.0 0.0 0.0 102-103 1.7875 0.0 0.0 0.0 0.0 104-105 1.9375 0.0 0.0 0.0 0.0 106-107 2.2125 0.0 0.0 0.0 0.0 108-109 2.6125 0.0 0.0 0.0 0.0 110-111 2.7875 0.0 0.0 0.0 0.0 112-113 2.9749999999999996 0.0 0.0 0.0 0.0 114-115 3.4124999999999996 0.0 0.0 0.0 0.0 116-117 3.8125 0.0 0.0 0.0 0.0 118-119 4.175000000000001 0.0 0.0 0.0 0.0 120-121 4.5125 0.0 0.0 0.0 0.0 122-123 5.050000000000001 0.0 0.0 0.0 0.0 124-125 5.4 0.0 0.0 0.0 0.0 126-127 5.6875 0.0 0.0 0.0 0.0 128-129 6.25 0.0 0.0 0.0 0.0 130-131 6.6125 0.0 0.0 0.0 0.0 132-133 7.0375 0.0 0.0 0.0 0.0 134-135 7.5 0.0 0.0 0.0 0.0 136-137 8.1125 0.0 0.0 0.0 0.0 138-139 8.525 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTTGCA 10 0.006830828 145.0 6 TCTCCCT 10 0.006830828 145.0 1 >>END_MODULE SRR13695432 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695432_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.22575 37.0 37.0 37.0 37.0 37.0 2 36.1885 37.0 37.0 37.0 37.0 37.0 3 36.1525 37.0 37.0 37.0 37.0 37.0 4 36.2815 37.0 37.0 37.0 37.0 37.0 5 36.2305 37.0 37.0 37.0 37.0 37.0 6 36.25 37.0 37.0 37.0 37.0 37.0 7 36.3035 37.0 37.0 37.0 37.0 37.0 8 36.401 37.0 37.0 37.0 37.0 37.0 9 36.292 37.0 37.0 37.0 37.0 37.0 10-14 36.32639999999999 37.0 37.0 37.0 37.0 37.0 15-19 36.2734 37.0 37.0 37.0 37.0 37.0 20-24 36.213699999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.158300000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.123000000000005 37.0 37.0 37.0 37.0 37.0 35-39 36.067899999999995 37.0 37.0 37.0 37.0 37.0 40-44 36.09 37.0 37.0 37.0 37.0 37.0 45-49 36.09025 37.0 37.0 37.0 37.0 37.0 50-54 36.0324 37.0 37.0 37.0 37.0 37.0 55-59 35.9753 37.0 37.0 37.0 37.0 37.0 60-64 35.960300000000004 37.0 37.0 37.0 37.0 37.0 65-69 35.944449999999996 37.0 37.0 37.0 37.0 37.0 70-74 35.9264 37.0 37.0 37.0 37.0 37.0 75-79 35.8976 37.0 37.0 37.0 37.0 37.0 80-84 35.93265 37.0 37.0 37.0 37.0 37.0 85-89 35.783550000000005 37.0 37.0 37.0 37.0 37.0 90-94 35.8138 37.0 37.0 37.0 37.0 37.0 95-99 35.768150000000006 37.0 37.0 37.0 37.0 37.0 100-104 35.7826 37.0 37.0 37.0 37.0 37.0 105-109 35.7503 37.0 37.0 37.0 37.0 37.0 110-114 35.734500000000004 37.0 37.0 37.0 37.0 37.0 115-119 35.5952 37.0 37.0 37.0 37.0 37.0 120-124 35.5495 37.0 37.0 37.0 37.0 37.0 125-129 35.517050000000005 37.0 37.0 37.0 37.0 37.0 130-134 35.427499999999995 37.0 37.0 37.0 34.6 37.0 135-139 35.422000000000004 37.0 37.0 37.0 34.6 37.0 140-144 35.2923 37.0 37.0 37.0 34.6 37.0 145-149 35.1118 37.0 37.0 37.0 29.8 37.0 150-151 34.96125 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 2.0 13 4.0 14 4.0 15 2.0 16 2.0 17 0.0 18 1.0 19 2.0 20 0.0 21 1.0 22 1.0 23 1.0 24 7.0 25 7.0 26 11.0 27 11.0 28 12.0 29 20.0 30 27.0 31 38.0 32 61.0 33 103.0 34 223.0 35 561.0 36 2726.0 37 172.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.50237678258694 22.39179384538404 13.785339004253188 27.32049036777583 2 30.075000000000003 25.1 28.775000000000002 16.05 3 20.0 28.050000000000004 31.525 20.424999999999997 4 25.25 32.175 23.7 18.875 5 25.674999999999997 36.85 20.9 16.575 6 21.825 40.025 21.3 16.85 7 21.825 21.7 37.7 18.775 8 22.225 26.125 26.075 25.575 9 22.075 25.674999999999997 28.999999999999996 23.25 10-14 23.095 29.265 26.275 21.365000000000002 15-19 23.385 28.225 27.07 21.32 20-24 23.10193057917375 29.16374912473742 27.493247974392315 20.24107232169651 25-29 23.2016008004002 27.808904452226113 28.154077038519258 20.83541770885443 30-34 23.44437775110044 28.49139655862345 27.921168467386952 20.143057222889155 35-39 23.342002600780233 28.123437031109333 27.70331099329799 20.831249374812444 40-44 23.584433773509403 27.866146458583437 27.561024409763906 20.988395358143258 45-49 22.307807732706447 29.450307607662683 27.44460561196419 20.797279047666684 50-54 22.726818045413623 28.133440032009606 27.54326297889367 21.596478943683106 55-59 23.29664832416208 28.019009504752372 27.648824412206103 21.03551775887944 60-64 23.532059617885366 27.068120436130837 27.97339201760528 21.426427928378512 65-69 23.865966491622906 27.341835458864715 27.551887971993 21.240310077519382 70-74 23.52176088044022 28.084042021010507 27.138569284642323 21.25562781390695 75-79 23.699479791916765 27.536014405762305 27.996198479391754 20.76830732292917 80-84 23.820955238809702 28.497124281070267 26.356589147286826 21.32533133283321 85-89 23.59561802811265 27.247261267570405 27.447351308088642 21.709769396228303 90-94 23.687106131839553 28.39351805541662 26.367910373111936 21.55146543963189 95-99 24.14603650912728 27.336834208552137 27.211802950737685 21.305326331582897 100-104 24.222111055527765 27.508754377188595 27.87393696848424 20.3951975987994 105-109 23.516758379189596 27.63381690845423 27.33866933466733 21.510755377688845 110-114 24.562368710613182 27.628288486545966 26.597979393818143 21.211363409022706 115-119 24.102051025512754 27.763881940970485 27.298649324662332 20.83541770885443 120-124 24.242121060530263 28.319159579789893 26.783391695847925 20.655327663831915 125-129 23.750687809514282 28.51783302486119 26.42689210144565 21.304587064178882 130-134 24.32216108054027 27.51375687843922 27.218609304652325 20.945472736368185 135-139 25.1975987993997 27.26863431715858 27.32366183091546 20.210105052526263 140-144 25.732719815944783 28.48854656396919 26.11283385015505 19.66589976993098 145-149 24.977488744372188 28.139069534767387 26.638319159579787 20.245122561280642 150-151 26.613306653326664 27.163581790895446 26.088044022011005 20.135067533766886 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.5 8 1.0 9 0.5 10 0.0 11 1.0 12 1.0 13 0.0 14 0.5 15 0.5 16 0.0 17 0.5 18 0.5 19 0.5 20 0.5 21 0.0 22 1.0 23 2.0 24 4.0 25 5.0 26 5.0 27 7.0 28 11.5 29 14.5 30 19.5 31 24.5 32 23.5 33 26.0 34 43.5 35 64.5 36 95.5 37 117.5 38 121.0 39 146.0 40 199.5 41 226.0 42 228.5 43 263.0 44 271.5 45 238.5 46 224.0 47 247.5 48 227.5 49 186.0 50 167.5 51 146.5 52 120.0 53 102.5 54 92.0 55 74.0 56 60.0 57 40.0 58 27.5 59 27.5 60 27.5 61 15.0 62 9.5 63 12.0 64 7.0 65 4.0 66 3.0 67 0.5 68 0.5 69 0.0 70 0.0 71 0.5 72 0.5 73 0.0 74 0.0 75 0.5 76 0.5 77 0.0 78 1.0 79 1.5 80 0.5 81 0.0 82 0.0 83 0.5 84 1.0 85 0.5 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.075 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.03 25-29 0.05 30-34 0.04 35-39 0.03 40-44 0.04 45-49 0.034999999999999996 50-54 0.03 55-59 0.05 60-64 0.03 65-69 0.025 70-74 0.05 75-79 0.04 80-84 0.025 85-89 0.045 90-94 0.03 95-99 0.025 100-104 0.05 105-109 0.05 110-114 0.03 115-119 0.05 120-124 0.05 125-129 0.045 130-134 0.05 135-139 0.05 140-144 0.03 145-149 0.05 150-151 0.05 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.15 #Duplication Level Percentage of deduplicated Percentage of total 1 72.48752672843905 50.849999999999994 2 18.56735566642908 26.05 3 5.452601568068425 11.475 4 2.138275124732716 6.0 5 0.7483962936564506 2.625 6 0.2851033499643621 1.2 7 0.1781895937277263 0.8750000000000001 8 0.03563791874554526 0.2 9 0.03563791874554526 0.22499999999999998 >10 0.07127583749109052 0.5 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTGAGCTTGAGTGCATTCTTCTAAGTAAAAGAAATCCCTTAATTTCATCA 10 0.25 No Hit GCCACAAAGACCCTTTTGATTTCTTACCGAAGGGTTTTTCGGACAGGACC 10 0.25 No Hit AGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATC 9 0.22499999999999998 No Hit GACTGGGCAAAACCAGGTTCCATGGGCAAGCAGTATTTCCTGGGATTCGA 8 0.2 No Hit AAATGAGGACATGGCAGCCGTGTTGAAGAAGGAAATGTCTGTAGCTCAAA 7 0.17500000000000002 No Hit TCCAATTCCATCAGGGGCTATTTCTGAGAATGAGGTTATTACCCAACTCT 7 0.17500000000000002 No Hit GTTAGAATTTATCCCCATGAAGAAGGAGATGTTGGTGTTATAACTAAAAG 7 0.17500000000000002 No Hit TGATACTGTTAATATACTGCAAAACCACTACCCTGAGAGGCTAGCCGTGG 7 0.17500000000000002 No Hit AGCTGGACCTCTTCGACCCATTGTACATGGCCAGACTTTGAAGTATAATA 7 0.17500000000000002 No Hit GGCTGGGGAATTGCGAGACAGAGAAATGGACCTTAGAGCACAGATTGCAG 6 0.15 No Hit CCCAATGGAAGAACTCATCTGACTACCACTCTTATAGCTATGGGAAGAAT 6 0.15 No Hit ATTCCTTGTGTCATTAATCATCTTAAGAATATATCTGGGATGGAGTTATG 6 0.15 No Hit GCTTTCCGTGTTCCTACCGTTGATGTCTCTGTTGTGGACCTCACAGTGAG 6 0.15 No Hit TCTGTATTGAGGAATAAAGAGTTCAAAGGAGCTTTAATTATATGCATGCA 6 0.15 No Hit GCCAGATACTCTCAAGGGAAAACCTGTGGTCTTAAAATATGTAAAGTTTC 6 0.15 No Hit TGGGAAGACATAGGAACCATCAGATCCTTCTTTGCAGCAAATCTTGCCCT 6 0.15 No Hit CCTGGGGTGCAAAATGGTGAGATGAGAACTAGTGACTCAGATTCTGACCA 6 0.15 No Hit CTCACAAAACAGTCCACAAACACCGCCAAATGGCCTCTGCCACAGCACCA 5 0.125 No Hit TGCTGAACATCAGGGTAAATATATGGCCTCAATGGTCAAGAGGCTTGCCC 5 0.125 No Hit GTTTAATGACTGTGGAAAATGGGAAATAATTTTGCAAGCATTTGTAATGC 5 0.125 No Hit GAACTCTCCGGCTTGATACCTCCAGAAATTGGATCACTGCAGTCTCTCAG 5 0.125 No Hit AGTAGTTGAGCGTTGTATCTTTGCTTTATCTCCCAAAACCTCTCCTCTCT 5 0.125 No Hit TGTTAAGAAACAAGAGCTAAAGGCTTTATATGACAAGGATTTTCAAGAAT 5 0.125 No Hit AGGCAAGCTTCATCACTCAGTGCCTTGTTCTTACAAGGAGATCTTTCGTC 5 0.125 No Hit GTTCGCTGAGGCCTGATTTTCATGTTGCGGCTCAAAATTGTTGGGTTAAG 5 0.125 No Hit GTGGTGAAATTTATAACGGCAAGGTGGATCACCCTTTGGGTCATCCTCCA 5 0.125 No Hit TCAAAAGTCTTGGCTTCGTCTCGCATGGGGCTTTGATAGTGAGCTTAGTT 5 0.125 No Hit ATCAGAGGGGCCTTCGAGTCCGTCGGTTCCTTCTTCACCGGCGGCGATCA 5 0.125 No Hit GTACAATCACAAGTCCGTAGAGACAGCGAGGAAGGGACAGGAAGTATGCA 5 0.125 No Hit ATATACTCTCCTGGCTTCTTTAGCAGGAGAGCACAACCAATCCTCACAAG 5 0.125 No Hit GGAAGAACTTCTATTCACGAGACGCGTTCCTTGAGGCTCTCAATTCATAC 5 0.125 No Hit GGTGGAACTTCAAAGAGACAATAGAACACCCTTCAAGTTTCATTCTGGTT 5 0.125 No Hit CCACCAGTCCCCAGGGTACTATGATGGACGCTACTGGACTATGTGGAAAC 5 0.125 No Hit ATATGGTCTGGTTGCAAGATCCTTTCCCGTATTTGGAGGGAAACCACGAT 5 0.125 No Hit GGAACAAGAAAAGGCTGATAGAGGTTTTGAGGAAGAGCAGCAAGCACAGG 5 0.125 No Hit GGGAGGTGTTGGTATTGACACGAGCATAACAATGATACGCAATCATGAGT 5 0.125 No Hit GACAAACACGTTGTCGAATCGTAAGCCGAAATGCAGATCTTCGTGAAGAC 5 0.125 No Hit CTTCACGTTCTTTAACAATCTTGCAGATTTAAGCTCTTAAAAATCTCTCT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.037500000000000006 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.0625 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.15 0.0 0.0 0.0 0.0 78-79 0.175 0.0 0.0 0.0 0.0 80-81 0.2125 0.0 0.0 0.0 0.0 82-83 0.275 0.0 0.0 0.0 0.0 84-85 0.35 0.0 0.0 0.0 0.0 86-87 0.425 0.0 0.0 0.0 0.0 88-89 0.55 0.0 0.0 0.0 0.0 90-91 0.6625 0.0 0.0 0.0 0.0 92-93 0.7124999999999999 0.0 0.0 0.0 0.0 94-95 0.825 0.0 0.0 0.0 0.0 96-97 0.9624999999999999 0.0 0.0 0.0 0.0 98-99 1.3250000000000002 0.0 0.0 0.0 0.0 100-101 1.5125000000000002 0.0 0.0 0.0 0.0 102-103 1.7875 0.0 0.0 0.0 0.0 104-105 1.9375 0.0 0.0 0.0 0.0 106-107 2.225 0.0 0.0 0.0 0.0 108-109 2.625 0.0 0.0 0.0 0.0 110-111 2.7875 0.0 0.0 0.0 0.0 112-113 2.9749999999999996 0.0 0.0 0.0 0.0 114-115 3.4124999999999996 0.0 0.0 0.0 0.0 116-117 3.8125 0.0 0.0 0.0 0.0 118-119 4.175000000000001 0.0 0.0 0.0 0.0 120-121 4.5125 0.0 0.0 0.0 0.0 122-123 5.1 0.0 0.0 0.0 0.0 124-125 5.449999999999999 0.0 0.0 0.0 0.0 126-127 5.7625 0.0 0.0 0.0 0.0 128-129 6.324999999999999 0.0 0.0 0.0 0.0 130-131 6.7125 0.0 0.0 0.0 0.0 132-133 7.175 0.0 0.0 0.0 0.0 134-135 7.675000000000001 0.0 0.0 0.0 0.0 136-137 8.2625 0.0 0.0 0.0 0.0 138-139 8.675 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGGGTCG 10 0.006830828 145.0 145 CCCCCCC 20 0.00593511 29.0 100-104 >>END_MODULE Read 881332 spots for SRR13695432.sra Written 881332 spots for SRR13695432.sra Read 881332 spots for SRR13695432.sra Written 881332 spots for SRR13695432.sra Read 881332 spots for SRR13695432.sra Written 881332 spots for SRR13695432.sra Read 881332 spots for SRR13695432.sra Written 881332 spots for SRR13695432.sra Read 881332 spots for SRR13695432.sra Written 881332 spots for SRR13695432.sra Read 881332 spots for SRR13695432.sra Written 881332 spots for SRR13695432.sra Read 881332 spots for SRR13695432.sra Written 881332 spots for SRR13695432.sra Read 881332 spots for SRR13695432.sra Written 881332 spots for SRR13695432.sra Read 881332 spots for SRR13695432.sra Written 881332 spots for SRR13695432.sra Read 881332 spots for SRR13695432.sra Written 881332 spots for SRR13695432.sra Read 881332 spots for SRR13695432.sra Written 881332 spots for SRR13695432.sra Read 881332 spots for SRR13695432.sra Written 881332 spots for SRR13695432.sra Read 881332 spots for SRR13695432.sra Written 881332 spots for SRR13695432.sra Read 881332 spots for SRR13695432.sra Written 881332 spots for SRR13695432.sra Read 881332 spots for SRR13695432.sra Written 881332 spots for SRR13695432.sra Read 881332 spots for SRR13695432.sra Written 881332 spots for SRR13695432.sra Read 881332 spots for SRR13695432.sra Written 881332 spots for SRR13695432.sra Read 881332 spots for SRR13695432.sra Written 881332 spots for SRR13695432.sra Read 881332 spots for SRR13695432.sra Written 881332 spots for SRR13695432.sra Read 881349 spots for SRR13695432.sra Written 881349 spots for SRR13695432.sra SRR ids: ['SRR13695432.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_sjk22ev_ SRR13695432.sra spots: 17626657 blocks: [[1, 881332], [881333, 1762664], [1762665, 2643996], [2643997, 3525328], [3525329, 4406660], [4406661, 5287992], [5287993, 6169324], [6169325, 7050656], [7050657, 7931988], [7931989, 8813320], [8813321, 9694652], [9694653, 10575984], [10575985, 11457316], [11457317, 12338648], [12338649, 13219980], [13219981, 14101312], [14101313, 14982644], [14982645, 15863976], [15863977, 16745308], [16745309, 17626657]] SRR13695432 file size 5968608 SRR13695432 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695432 SRR13695432_1.fastq SRR13695432_2.fastq Input file: SRR13695432_1.fastq Paired file: SRR13695432_2.fastq trimmed: SRR13695432-trimmed-pair1.fastq, SRR13695432-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 02:51:46 2025 >> started Wed Feb 12 02:52:04 2025 >> done (18.975s) 17626657 read pairs processed; of these: 117 ( 0.00%) short read pairs filtered out after trimming by size control 1972 ( 0.01%) empty read pairs filtered out after trimming by size control 17624568 (99.99%) read pairs available; of these: 2286880 (12.98%) trimmed read pairs available after processing 15337688 (87.02%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 5 0.00% 20 4 0.00% 21 4 0.00% 22 4 0.00% 23 7 0.00% 24 9 0.00% 25 2 0.00% 26 4 0.00% 27 4 0.00% 28 3 0.00% 29 5 0.00% 30 8 0.00% 31 4 0.00% 32 13 0.00% 33 9 0.00% 34 9 0.00% 35 8 0.00% 36 16 0.00% 37 11 0.00% 38 16 0.00% 39 13 0.00% 40 13 0.00% 41 15 0.00% 42 26 0.00% 43 11 0.00% 44 13 0.00% 45 21 0.00% 46 26 0.00% 47 29 0.00% 48 27 0.00% 49 51 0.00% 50 32 0.00% 51 78 0.00% 52 72 0.00% 53 65 0.00% 54 68 0.00% 55 101 0.00% 56 104 0.00% 57 122 0.00% 58 143 0.00% 59 169 0.00% 60 226 0.00% 61 224 0.00% 62 300 0.00% 63 353 0.00% 64 366 0.00% 65 414 0.00% 66 455 0.00% 67 544 0.00% 68 605 0.00% 69 791 0.00% 70 862 0.00% 71 1026 0.01% 72 1158 0.01% 73 1371 0.01% 74 1515 0.01% 75 1743 0.01% 76 2093 0.01% 77 2051 0.01% 78 2436 0.01% 79 2857 0.02% 80 3063 0.02% 81 3542 0.02% 82 4141 0.02% 83 4485 0.03% 84 5232 0.03% 85 5842 0.03% 86 6258 0.04% 87 6664 0.04% 88 7301 0.04% 89 7904 0.04% 90 8519 0.05% 91 9589 0.05% 92 9820 0.06% 93 10825 0.06% 94 11958 0.07% 95 12881 0.07% 96 13877 0.08% 97 14259 0.08% 98 15565 0.09% 99 16168 0.09% 100 17208 0.10% 101 17221 0.10% 102 18866 0.11% 103 19665 0.11% 104 20715 0.12% 105 22119 0.13% 106 23146 0.13% 107 23616 0.13% 108 24747 0.14% 109 25774 0.15% 110 26307 0.15% 111 27111 0.15% 112 28264 0.16% 113 28910 0.16% 114 31025 0.18% 115 31834 0.18% 116 32729 0.19% 117 34259 0.19% 118 35318 0.20% 119 35478 0.20% 120 37079 0.21% 121 37888 0.21% 122 38537 0.22% 123 39134 0.22% 124 41031 0.23% 125 41543 0.24% 126 42983 0.24% 127 43976 0.25% 128 44568 0.25% 129 45637 0.26% 130 46383 0.26% 131 46945 0.27% 132 47613 0.27% 133 48074 0.27% 134 48722 0.28% 135 50206 0.28% 136 50940 0.29% 137 52590 0.30% 138 53419 0.30% 139 54830 0.31% 140 55295 0.31% 141 56006 0.32% 142 56489 0.32% 143 57041 0.32% 144 58001 0.33% 145 58495 0.33% 146 59245 0.34% 147 60511 0.34% 148 62156 0.35% 149 62786 0.36% 150 63817 0.36% 151 15337688 87.02% 17624568 reads passed initial QC criterion=sequence-density sequence-density=0.29 sequence-density-rank=1 fanout-score=5.61 fanout-score-rank=6 prefix-density=0.54 prefix-fanout=3.0 sequence=TTGCAGCCATTCTC criterion=fanout-score sequence-density=0.02 sequence-density-rank=33 fanout-score=25.63 fanout-score-rank=1 prefix-density=0.08 prefix-fanout=6.4 sequence=AATGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGTTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGAGTATGAATGTGTTCTCTGTG criterion=sequence-density sequence-density=0.46 sequence-density-rank=1 fanout-score=2.36 fanout-score-rank=28 prefix-density=0.48 prefix-fanout=2.3 sequence=TGCAAGTGCGGCAG criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=35.10 fanout-score-rank=1 prefix-density=0.04 prefix-fanout=5.8 sequence=CTTCAAAAGTACTACCAACTCCTTCGCCATGCAGAAACTCGCTTGTCTGGTCCTCGCCGCTCTGGCCTTCTTCGC SRR13695432 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 02:52:53 Started mapping on | Feb 12 02:52:54 Finished on | Feb 12 02:57:44 Mapping speed, Million of reads per hour | 218.79 Number of input reads | 17624568 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 14824339 Uniquely mapped reads % | 84.11% Average mapped length | 294.01 Number of splices: Total | 13871258 Number of splices: Annotated (sjdb) | 13506678 Number of splices: GT/AG | 13581721 Number of splices: GC/AG | 208433 Number of splices: AT/AC | 9820 Number of splices: Non-canonical | 71284 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 2.98 Insertion rate per base | 0.02% Insertion average length | 2.36 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 446548 % of reads mapped to multiple loci | 2.53% Number of reads mapped to too many loci | 121620 % of reads mapped to too many loci | 0.69% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 12.43% % of reads unmapped: other | 0.23% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 2353866 2353866 2353866 N_multimapping 446548 446548 446548 N_noFeature 607658 14412924 829499 N_ambiguous 309214 2090 118023 UnstrandedReadsAssigned:13907467 PositiveStrandReadsAssigned:409325 NegativeStrandReadsAssigned:13876817 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695432 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695432-trimmed-pair1.fastq SRR13695432-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 17,624,568 reads, 13,965,549 reads pseudoaligned [quant] estimated average fragment length: 242.692 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,095 rounds 52401 SRR13695432.ke.tsv 34699 SRR13695432.se.tsv 87100 total ==> SRR13695432.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1776.31 581 18.1895 Potri.005G024800.1.v4.1 1035 793.308 462 32.3864 Potri.004G059700.1.v4.1 961 719.403 0 0 Potri.007G009000.2.v4.1 1416 1174.31 0 0 Potri.003G141000.2.v4.1 2943 2701.31 799 16.4489 Potri.016G087400.1.v4.1 270 87.2772 815 519.301 Potri.015G069301.1.v4.1 564 330.452 0 0 Potri.010G195200.1.v4.1 1773 1531.31 344 12.4928 Potri.012G127500.1.v4.1 977 735.342 154 11.6465 ==> SRR13695432.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 176 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 291 Potri.001G212900.v4.1 502 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 5 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 3 SRR13695432 completed mapping pipeline successfully