Starting /dee2/code/volunteer_pipeline.sh SRR13695433 current disk space = 3049497088000 free memory = 997427216 SRR13695433 SRAfilesize d44d4584fffbe0f7d113bbca19813f20 SRR13695433.sra SRR13695433.sra file validated SRR13695433 is paired end SRR13695433 is conventional basespace SRR13695433 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695433_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.575 37.0 37.0 37.0 37.0 37.0 2 36.29475 37.0 37.0 37.0 37.0 37.0 3 36.553 37.0 37.0 37.0 37.0 37.0 4 36.6275 37.0 37.0 37.0 37.0 37.0 5 36.6085 37.0 37.0 37.0 37.0 37.0 6 36.507 37.0 37.0 37.0 37.0 37.0 7 36.5065 37.0 37.0 37.0 37.0 37.0 8 36.4655 37.0 37.0 37.0 37.0 37.0 9 36.6 37.0 37.0 37.0 37.0 37.0 10-14 36.5319 37.0 37.0 37.0 37.0 37.0 15-19 36.5072 37.0 37.0 37.0 37.0 37.0 20-24 36.507999999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.43150000000001 37.0 37.0 37.0 37.0 37.0 30-34 36.42999999999999 37.0 37.0 37.0 37.0 37.0 35-39 36.4661 37.0 37.0 37.0 37.0 37.0 40-44 36.424099999999996 37.0 37.0 37.0 37.0 37.0 45-49 36.3609 37.0 37.0 37.0 37.0 37.0 50-54 36.3815 37.0 37.0 37.0 37.0 37.0 55-59 36.35979999999999 37.0 37.0 37.0 37.0 37.0 60-64 36.3304 37.0 37.0 37.0 37.0 37.0 65-69 36.296 37.0 37.0 37.0 37.0 37.0 70-74 36.284000000000006 37.0 37.0 37.0 37.0 37.0 75-79 36.242200000000004 37.0 37.0 37.0 37.0 37.0 80-84 36.1732 37.0 37.0 37.0 37.0 37.0 85-89 36.132400000000004 37.0 37.0 37.0 37.0 37.0 90-94 36.1279 37.0 37.0 37.0 37.0 37.0 95-99 36.03439999999999 37.0 37.0 37.0 37.0 37.0 100-104 36.0424 37.0 37.0 37.0 37.0 37.0 105-109 36.0687 37.0 37.0 37.0 37.0 37.0 110-114 36.0578 37.0 37.0 37.0 37.0 37.0 115-119 35.98909999999999 37.0 37.0 37.0 37.0 37.0 120-124 35.935500000000005 37.0 37.0 37.0 37.0 37.0 125-129 35.8929 37.0 37.0 37.0 37.0 37.0 130-134 35.9495 37.0 37.0 37.0 37.0 37.0 135-139 35.814800000000005 37.0 37.0 37.0 37.0 37.0 140-144 35.7376 37.0 37.0 37.0 37.0 37.0 145-149 35.5674 37.0 37.0 37.0 37.0 37.0 150-151 35.518 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 1.0 23 2.0 24 2.0 25 3.0 26 3.0 27 4.0 28 18.0 29 19.0 30 33.0 31 45.0 32 52.0 33 86.0 34 126.0 35 350.0 36 2893.0 37 363.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.875 11.600000000000001 8.475000000000001 39.050000000000004 2 21.893045443133317 13.959327140346472 35.65151895556114 28.496108460959075 3 19.975 18.45 26.400000000000002 35.175 4 21.25 25.825 23.875 29.049999999999997 5 23.549999999999997 31.45 24.425 20.575 6 22.3 34.949999999999996 23.3 19.45 7 14.7 26.174999999999997 41.475 17.65 8 17.25 26.674999999999997 32.25 23.825 9 18.575 25.25 33.925 22.25 10-14 20.21 29.235 27.76 22.795 15-19 19.645000000000003 28.425 28.205000000000002 23.724999999999998 20-24 20.325 28.62 27.74 23.315 25-29 20.265 28.575 28.38 22.78 30-34 19.975 29.13 26.985 23.91 35-39 19.695 28.845 27.99 23.47 40-44 19.939999999999998 28.595 27.71 23.755000000000003 45-49 19.555 28.825 28.08 23.54 50-54 20.035 28.055000000000003 28.694999999999997 23.215 55-59 19.755 28.205000000000002 28.4 23.64 60-64 20.155 28.675 27.92 23.25 65-69 19.24 28.585 28.84 23.335 70-74 20.665 28.96 27.82 22.555 75-79 20.23 28.64 27.92 23.21 80-84 19.615 28.51 27.860000000000003 24.015 85-89 20.525 28.825 26.979999999999997 23.669999999999998 90-94 20.49 28.225 27.47 23.815 95-99 20.375 29.125 26.795 23.705000000000002 100-104 20.715 28.82 27.134999999999998 23.330000000000002 105-109 19.575 28.994999999999997 28.275 23.155 110-114 20.474999999999998 28.310000000000002 27.32 23.895 115-119 20.630000000000003 28.645 27.12 23.605 120-124 21.22 28.075 27.21 23.494999999999997 125-129 20.03 28.79 26.950000000000003 24.23 130-134 19.725 28.535 27.505000000000003 24.235 135-139 21.11 28.825 26.275 23.79 140-144 20.91 29.845 26.13 23.115 145-149 20.53 28.060000000000002 26.974999999999998 24.435000000000002 150-151 21.125 29.2 26.224999999999998 23.45 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.0 19 1.0 20 1.0 21 1.0 22 2.5 23 1.5 24 0.0 25 3.5 26 6.0 27 5.0 28 15.5 29 20.0 30 18.5 31 28.5 32 39.5 33 53.0 34 56.5 35 82.5 36 95.0 37 87.5 38 121.0 39 166.0 40 206.0 41 227.5 42 229.0 43 246.5 44 286.0 45 294.0 46 258.0 47 232.5 48 226.5 49 199.5 50 159.5 51 132.0 52 106.0 53 87.0 54 74.0 55 52.5 56 38.0 57 40.0 58 30.0 59 18.0 60 17.5 61 11.0 62 7.5 63 4.5 64 1.0 65 1.0 66 3.5 67 2.5 68 0.0 69 0.0 70 1.0 71 1.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.42500000000000004 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.47500000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 66.96393951143854 43.175000000000004 2 20.43427685149283 26.35 3 7.444746025591314 14.399999999999999 4 2.6754556029468786 6.9 5 1.5509887553315238 5.0 6 0.4265219077161691 1.6500000000000001 7 0.27142303218301667 1.225 8 0.11632415664986429 0.6 9 0.07754943776657619 0.44999999999999996 >10 0.038774718883288095 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TCCATGTGCTTGCAGGGCCCACACCAAGATGCCGCGAAATCAATCACCAT 10 0.25 No Hit ATTTCTCCTTCACCTGGTAGTGTTATTGCCTTTGCAATAAATTCCTTATC 9 0.22499999999999998 No Hit GATCGTTAGCCCAAATATTCCTTCCATCAACCACTCCAGCAAAGAGGTAT 9 0.22499999999999998 No Hit CCATTATTCTTAGTTGTAAAGGACTCTCTATATACCATCCCATGCAACAC 8 0.2 No Hit CCACAAAGCATATTCTATATTCAATATCAGCAACATAAAAATACTGATCC 8 0.2 No Hit CCAGGAATACTTGATTTGCTTGAACTGTTGTCCATTGGCTCCATGCACTC 8 0.2 No Hit GGCAAAAATATTACAGTTCTGGAAAACCACTGCTGAATTACCAAAAATGA 7 0.17500000000000002 No Hit CCTGTTTGCATCAGCTAAATCTCGCAAATAACAAACATTTTCCGCATCTG 7 0.17500000000000002 No Hit GCACGGCCTTATAATATAGCTCTACATTAGCAACTTTGACAGCAACATCT 7 0.17500000000000002 No Hit CTTAAGTTTTTCTTGAGCCCTGTAGCAAAAAATAAAAAAGCAGACTAATG 7 0.17500000000000002 No Hit GTTTGAAAACAATTACTTGCATTAGGATTCTGTGTCCAGTTAGCCCAGTT 7 0.17500000000000002 No Hit CTTCCATTAGGCATGTAGTCATATACCAAAAGTAGCTCTCCTCTACGCCG 7 0.17500000000000002 No Hit CCGGACTCATATCTCGATAACAAGTGAAAACAAAGCCTCCAACTGTCAAA 7 0.17500000000000002 No Hit CAGAAAACCGGCAAAGACCATTCCCTTCAAGCCGAATGCCCGCTGATGCC 6 0.15 No Hit CTGCCAGTAGCTCCTCTACACTGCCGAGTGGAACTAAAGACTGTCGAGCT 6 0.15 No Hit ATTTTGGTTTTCATTTTCTTGTTTCTATCCACTATCGTTTCTTCATTCCA 6 0.15 No Hit GGTCATAGGCATTTGATTTATGCTTCGGTGCAAATGTAAAGGCATCCGGA 6 0.15 No Hit CCAAAGCACATGCATGGAAATAGACAGGCAGTGAGAATCATCGACTTGAT 6 0.15 No Hit CTTCATAAAGCTTGCTGGTATTTGACACATCTTCAATGTCATCAAATTCA 6 0.15 No Hit TCGCTCTGTCCCAGCTGATTTCAGGAGCTCTATCTACATGTTGTAGAGAT 6 0.15 No Hit GGAATATTTTGTGGATGTAGAAGGGTTTCAGAGAGCTTCACTATTTCATC 6 0.15 No Hit GTGAGGTTAAGGAAATCGTTTGCCATGGCCAAGGCACGTCCCATCGCTTG 6 0.15 No Hit TGGTAATATATCATGCACTGCAAGGAAAACCTCTGCAGAGAAGCCTAAAA 6 0.15 No Hit GGGGAGATGTCGATTTTCCAAACAGGGAGCACCATGTAGGGAGTTTTGCA 6 0.15 No Hit GTGAGTCTGGTCTTGTTTGAAGTGAAGGTAGCAGATGGGATTTTGAAGAG 5 0.125 No Hit AGTCAGAGATAAATGATGTATGATCCATGGATTCTCCAGGGCATTCCTCA 5 0.125 No Hit TGGAGAAAATGCTCAAAAAGCCAGGCTCTTAGAATGTTGACAGATCGTTC 5 0.125 No Hit GCATCTACTCGGACTCCAATAACATCACCGGCCAAATTCTTTGCCAAGTT 5 0.125 No Hit CAACAGTGTGGCCCTTCTTTGGTTTTGCAACATTCAAAGCTGCGCCAAGG 5 0.125 No Hit GGAGCATTACTAAATGACACACGAGCCAGCCCAAAGTCACAAAGCTTTAA 5 0.125 No Hit GTCACGTACTACTGAGACATACATGTTGTGCACCATATACATATAGTAGT 5 0.125 No Hit CTCCCCTTCACCAAGCTCTAACAAACACAAATACGCATTTAATTATATAG 5 0.125 No Hit GTTCTGATCACCAAAAAATTGGGCTGATGAGATTGATTTTGCATTTGAGA 5 0.125 No Hit CTCCAAACTAGGTGGCCCTATGTTTTTTTTATTCCCCTTTGAATCAACCT 5 0.125 No Hit CAACAACAACAACGACAACGATGATATGATATTAAGTAGTTAAAAAAAGA 5 0.125 No Hit CTTGAGATTCCTCTGGTTGAGCACTCATTGAACCATTTGAGATAGCAAAT 5 0.125 No Hit GTCGGAATTATCAGAGAGGCGAAGAAATGGGAAAGGACTACAACGAGGCC 5 0.125 No Hit GAATAGGTTAGTTCAAGACAATAACATTTGCAAAGAGTACTAAAATTCTA 5 0.125 No Hit GTCCACAGTTTTCCTGCTGCTGCAAGAATCTTTTTGGTCATCTCGGGAGT 5 0.125 No Hit GTCATGCCTTTCATGTAGAAGTCCCTGGCGCTGGTCAAAATCTTGAGGGG 5 0.125 No Hit GGATTCTGTAACTGCTTACAACACCACCTGCAGAAAAAATCTCAAATCAT 5 0.125 No Hit GGCGTTCCTTGTTAGAGGAGGTTATTGGGTGTATCAAAGGATCATAATAG 5 0.125 No Hit CTCACGGTTCTTATACCTCTTATCTTGAAGAACTTTCTTTATAGCAACAG 5 0.125 No Hit CAGACATTTACAAAATAGCAGCAGCTTTACTGTATTTAGCAAAACTACCA 5 0.125 No Hit TGAATGTCGTGACAATAGCTCCTCTCTCTTCAGATTAAAAGTTCTTTCAC 5 0.125 No Hit CGCATTTTCATCCAATCCACGAACAACCAAAACATGAGTTGGGCCTGCCT 5 0.125 No Hit CCCATCTCTAGCCACCTCTGGATTGTCAAACCTCTCAGGTCTGAATTTTG 5 0.125 No Hit GTCCAAATCCTCATAATTGCAAGAGGACCAAGAAACCTTGGGCCAAGTAC 5 0.125 No Hit CTTTGTTCTTCCTCTTAAGTTCCACAACTTCTACCTCCAACTCCTTCACA 5 0.125 No Hit CTCTTGTTTAAGCGCAAACCTACAGCTTCTAGTTCCCTGGTTAATATTTG 5 0.125 No Hit CCCAGTCTGGCATTTCCGATATCTTGACAAACCTCTCATCAGGAGGTATC 5 0.125 No Hit CAGAGAGCGGGGGGAGATCTTCACCTTCCTGTTGGCTGTGGACAACGCCG 5 0.125 No Hit CTCCAAACAAATCAACTTGGTTTGAGGTTTGAGAAGCAGACTTAGTGCTG 5 0.125 No Hit TTGCAATGCAAGATAGATGATATGCCTAAGACAAATAAACAGAGTATAGC 5 0.125 No Hit GCTGCTTGGTCCAAGAAATTGGCCAATGAATGAAACACTAATTGTCTTGC 5 0.125 No Hit CACCAATCTTTGGTTCAACCTTTGAGGAACTACAGTCCTCAACAATTTTG 5 0.125 No Hit GGCAGCTTCTTTCAACACATGCCCTTCAAGCCCTAACCCTAAAGAGAGGG 5 0.125 No Hit CAGCAGGCCAAAGTTCATCCCCGAAGAAGCTACTAGTGTAGAGCACCTGG 5 0.125 No Hit GCATGTATGCCTTCATATGCTTGAATATCAAAATATCAAGAGGTACTTTT 5 0.125 No Hit CAGAGCGTCAAAAAAAGACAAAGCAGAGTGGATTAGTATATTATCAAGAG 5 0.125 No Hit CCGCGAATGTATTGCGAGAGTCATCAGAACCTTGATCTATGCAGGGAAGA 5 0.125 No Hit TTTTTTTTTACCAAACAGAACACACTGCAAGTTAACATATTATAACCAAA 5 0.125 No Hit GTCAACGTAATCTAGCAACATATGCAAGAGACACTGCTGCAGCAACAACT 5 0.125 No Hit CAACATTATAAGATTGTAGCCCTCTGTTACTGCTGCTCAAATCGTTGTTG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.037500000000000006 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.225 0.0 0.0 0.0 0.0 80-81 0.35 0.0 0.0 0.0 0.0 82-83 0.4 0.0 0.0 0.0 0.0 84-85 0.475 0.0 0.0 0.0 0.0 86-87 0.55 0.0 0.0 0.0 0.0 88-89 0.625 0.0 0.0 0.0 0.0 90-91 0.6875 0.0 0.0 0.0 0.0 92-93 0.8625 0.0 0.0 0.0 0.0 94-95 1.0499999999999998 0.0 0.0 0.0 0.0 96-97 1.1124999999999998 0.0 0.0 0.0 0.0 98-99 1.275 0.0 0.0 0.0 0.0 100-101 1.525 0.0 0.0 0.0 0.0 102-103 1.8625 0.0 0.0 0.0 0.0 104-105 2.15 0.0 0.0 0.0 0.0 106-107 2.4 0.0 0.0 0.0 0.0 108-109 2.6875 0.0 0.0 0.0 0.0 110-111 3.1 0.0 0.0 0.0 0.0 112-113 3.6 0.0 0.0 0.0 0.0 114-115 3.875 0.0 0.0 0.0 0.0 116-117 4.2 0.0 0.0 0.0 0.0 118-119 4.7 0.0 0.0 0.0 0.0 120-121 5.15 0.0 0.0 0.0 0.0 122-123 5.775 0.0 0.0 0.0 0.0 124-125 6.2375 0.0 0.0 0.0 0.0 126-127 6.65 0.0 0.0 0.0 0.0 128-129 7.25 0.0 0.0 0.0 0.0 130-131 7.8125 0.0 0.0 0.0 0.0 132-133 8.399999999999999 0.0 0.0 0.0 0.0 134-135 8.8625 0.0 0.0 0.0 0.0 136-137 9.6625 0.0 0.0 0.0 0.0 138-139 10.4125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAAATAA 10 0.006830828 145.0 9 TATAAGG 10 0.006830828 145.0 9 TCAATTG 10 0.006830828 145.0 3 GGTCAAT 10 0.006830828 145.0 1 ATTATAA 10 0.006830828 145.0 5 GTCTCCA 10 0.006830828 145.0 9 ATTGAAA 10 0.006830828 145.0 6 TTGAAAT 10 0.006830828 145.0 7 AGTCTCC 10 0.006830828 145.0 8 TGAAATA 10 0.006830828 145.0 8 >>END_MODULE SRR13695433 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695433_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.097 37.0 37.0 37.0 37.0 37.0 2 36.0985 37.0 37.0 37.0 37.0 37.0 3 36.1635 37.0 37.0 37.0 37.0 37.0 4 36.236 37.0 37.0 37.0 37.0 37.0 5 36.283 37.0 37.0 37.0 37.0 37.0 6 36.345 37.0 37.0 37.0 37.0 37.0 7 36.3335 37.0 37.0 37.0 37.0 37.0 8 36.195 37.0 37.0 37.0 37.0 37.0 9 36.262 37.0 37.0 37.0 37.0 37.0 10-14 36.226800000000004 37.0 37.0 37.0 37.0 37.0 15-19 36.185300000000005 37.0 37.0 37.0 37.0 37.0 20-24 36.141099999999994 37.0 37.0 37.0 37.0 37.0 25-29 36.1044 37.0 37.0 37.0 37.0 37.0 30-34 36.04915 37.0 37.0 37.0 37.0 37.0 35-39 36.017399999999995 37.0 37.0 37.0 37.0 37.0 40-44 36.015299999999996 37.0 37.0 37.0 37.0 37.0 45-49 36.091899999999995 37.0 37.0 37.0 37.0 37.0 50-54 35.9659 37.0 37.0 37.0 37.0 37.0 55-59 35.9469 37.0 37.0 37.0 37.0 37.0 60-64 35.9155 37.0 37.0 37.0 37.0 37.0 65-69 35.8786 37.0 37.0 37.0 37.0 37.0 70-74 35.8326 37.0 37.0 37.0 37.0 37.0 75-79 35.8384 37.0 37.0 37.0 37.0 37.0 80-84 35.7909 37.0 37.0 37.0 37.0 37.0 85-89 35.73030000000001 37.0 37.0 37.0 37.0 37.0 90-94 35.7042 37.0 37.0 37.0 37.0 37.0 95-99 35.7123 37.0 37.0 37.0 37.0 37.0 100-104 35.6528 37.0 37.0 37.0 37.0 37.0 105-109 35.6951 37.0 37.0 37.0 37.0 37.0 110-114 35.6349 37.0 37.0 37.0 37.0 37.0 115-119 35.6306 37.0 37.0 37.0 37.0 37.0 120-124 35.5234 37.0 37.0 37.0 37.0 37.0 125-129 35.4848 37.0 37.0 37.0 37.0 37.0 130-134 35.3446 37.0 37.0 37.0 32.2 37.0 135-139 35.31105 37.0 37.0 37.0 32.2 37.0 140-144 35.236000000000004 37.0 37.0 37.0 34.6 37.0 145-149 35.06 37.0 37.0 37.0 27.4 37.0 150-151 34.720749999999995 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 3.0 14 6.0 15 0.0 16 5.0 17 2.0 18 4.0 19 0.0 20 1.0 21 3.0 22 5.0 23 5.0 24 6.0 25 5.0 26 9.0 27 16.0 28 16.0 29 16.0 30 17.0 31 53.0 32 68.0 33 100.0 34 214.0 35 607.0 36 2637.0 37 200.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 44.83967935871743 16.082164328657313 13.151302605210422 25.926853707414832 2 28.349999999999998 21.025 34.25 16.375 3 20.225 25.124999999999996 32.6 22.05 4 23.7 33.650000000000006 23.575 19.075 5 25.624999999999996 34.275 21.375 18.725 6 21.175 38.025 22.525000000000002 18.275 7 21.45 21.75 37.775 19.025 8 21.425 27.175 27.55 23.849999999999998 9 24.075 23.7 30.675 21.55 10-14 23.195 29.154999999999998 27.04 20.61 15-19 23.064999999999998 28.345 27.55 21.04 20-24 23.02730273027303 28.352835283528353 28.03780378037804 20.582058205820584 25-29 23.176588294147074 28.609304652326163 28.419209604802404 19.794897448724363 30-34 22.260565141285323 28.417104276069015 28.28207051762941 21.040260065016252 35-39 22.912291229122914 27.652765276527653 28.79287928792879 20.642064206420642 40-44 22.656797039111733 27.81834550365109 28.44853456036811 21.07632289686906 45-49 22.214442888577715 27.730546109221844 29.170834166833366 20.884176835367075 50-54 23.18231823182318 28.32783278327833 28.072807280728075 20.417041704170416 55-59 22.746373186593296 28.084042021010507 28.01400700350175 21.155577788894448 60-64 23.552355235523553 28.17781778177818 27.642764276427645 20.627062706270628 65-69 23.45 27.615000000000002 27.92 21.015 70-74 23.07153576788394 27.973986993496748 28.23911955977989 20.715357678839418 75-79 23.1519455836751 27.618285485645693 28.103431029308794 21.126337901370412 80-84 23.21 28.76 27.605 20.424999999999997 85-89 22.95918367346939 28.886554621848738 27.73609443777511 20.41816726690676 90-94 23.612361236123615 27.797779777977798 27.927792779277926 20.662066206620665 95-99 24.16 28.07 26.69 21.08 100-104 23.911955977988995 27.153576788394197 28.209104552276138 20.72536268134067 105-109 23.08654327163582 27.743871935967984 29.239619809904955 19.929964982491246 110-114 23.597359735973598 28.12281228122812 27.447744774477446 20.83208320832083 115-119 23.37434973989596 28.44637855142057 27.656062424969992 20.523209283713488 120-124 24.62231115557779 27.913956978489246 27.823911955977987 19.639819909954976 125-129 25.46018407362945 27.55102040816326 27.07082833133253 19.917967186874748 130-134 25.15009005403242 27.80168100860516 27.06623974384631 19.98198919351611 135-139 26.04932712992146 27.965380959527742 26.13937665716144 19.845915253389364 140-144 25.54755475547555 28.02280228022802 27.06770677067707 19.36193619361936 145-149 26.588294147073537 27.54377188594297 26.563281640820406 19.304652326163083 150-151 27.282962221666253 27.845884413309985 26.607455591693768 18.263697773329998 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 0.5 11 0.5 12 1.0 13 0.5 14 0.0 15 0.0 16 0.5 17 1.0 18 0.5 19 0.0 20 0.0 21 1.5 22 3.0 23 2.5 24 3.5 25 6.5 26 9.5 27 10.5 28 10.5 29 15.5 30 21.5 31 23.0 32 24.0 33 34.5 34 51.0 35 75.0 36 83.5 37 99.5 38 135.0 39 168.5 40 207.0 41 237.0 42 234.5 43 245.0 44 294.5 45 274.0 46 253.0 47 261.5 48 227.5 49 171.5 50 152.0 51 152.0 52 116.5 53 79.5 54 65.5 55 67.0 56 54.5 57 36.0 58 22.0 59 13.0 60 13.0 61 13.0 62 7.5 63 3.5 64 2.0 65 1.0 66 0.0 67 1.0 68 1.0 69 0.0 70 0.0 71 0.0 72 1.0 73 1.5 74 0.5 75 0.5 76 0.5 77 0.0 78 1.0 79 1.0 80 0.0 81 0.0 82 0.0 83 0.5 84 0.5 85 0.0 86 0.5 87 0.5 88 0.0 89 0.5 90 0.5 91 0.0 92 0.5 93 1.0 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.01 25-29 0.05 30-34 0.025 35-39 0.01 40-44 0.03 45-49 0.02 50-54 0.01 55-59 0.05 60-64 0.01 65-69 0.0 70-74 0.05 75-79 0.03 80-84 0.0 85-89 0.04 90-94 0.01 95-99 0.0 100-104 0.05 105-109 0.05 110-114 0.01 115-119 0.04 120-124 0.05 125-129 0.04 130-134 0.06 135-139 0.055 140-144 0.01 145-149 0.05 150-151 0.075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.225 #Duplication Level Percentage of deduplicated Percentage of total 1 68.1103871215025 44.425 2 19.586048294365657 25.55 3 7.474128018397853 14.625 4 2.414718282866999 6.3 5 1.4564967420467612 4.75 6 0.42161747796090454 1.6500000000000001 7 0.2683020314296665 1.225 8 0.19164430816404754 1.0 9 0.038328861632809505 0.22499999999999998 >10 0.038328861632809505 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AAAAAAAACACACAGAGAGAAGAAAGATGGGAGCCGTACTGTCTTCCATT 10 0.25 No Hit GTCTTGAATCTGATCTCTCTGACAGTGATCTGTTTTTCCTCCTTGCTCAT 9 0.22499999999999998 No Hit TTTACTTAGCTGCAGCTCCACAATGTCCCTTCCCCGATGCTCATCTAGAC 8 0.2 No Hit ACCACATATAGAGGGTGTAATAGCTAAGTAGCCTGTAAGAGATGGCTTCC 8 0.2 No Hit CTTCTCTCCCTAATTGACAAGATTCTTCCCGTCTATCAGGAAGTTGTGGC 8 0.2 No Hit GTGGTGGATTACGATCAGTGATTGAGCCAGATTCGAGTAATGTGTAAAGC 8 0.2 No Hit AGTAAGTTGAGCTGTTGTATGTGTGTTTCTAAGTGGATAAGGTATGGTTA 8 0.2 No Hit GCTGTTCTTGTCCAGTACATTCCAAGGCTGTGGAGGTTTACTCCTCTTCT 7 0.17500000000000002 No Hit CCAGCAGAAGGGAGGGGTACGTGACTTTGTTTCACTCCACAACTCTGTTG 7 0.17500000000000002 No Hit GTTGGGTAGTGGTGGGTTTGGTAGAGTCTACAAAGGTGTCTTGCCAACCT 7 0.17500000000000002 No Hit CGGATGGAAAAATAGCAGCAGGGGACAACGTAGATAGGAGCCGCCCATGA 7 0.17500000000000002 No Hit GACACTACTAACTGCAGTTGGCGAGACTATAAGCTACAAGATTCTCATTA 7 0.17500000000000002 No Hit AATGCAAATAAATGTGCGTAGTCTGAACTGGCTTTGAGGAATTTTTGAGG 7 0.17500000000000002 No Hit AGCACCAAGCTGTTGCCCTTCGAGTTGGATCCGATAGAGCAGTAATCTTT 7 0.17500000000000002 No Hit AATCACAAATCTGACTGCCCTAAGCTTTCTTCATGCATGATAGCTAGAGG 6 0.15 No Hit GGGAAAGAGTCACCAAGGAAGTTTGAGCTAACTCTTTTCTGGTCAGTAAA 6 0.15 No Hit GTTCTTCCCTTCTTTCCTGCAGACAGAAAGTAGTTCTGCTGAGGCCAAAG 6 0.15 No Hit GTTTGACAATCATTTCTTTGAAGATGTTGGAGGTGGTGTTGAACGATCGT 6 0.15 No Hit GAAATTCCTGGTGCACAAAACATACTTCAGATGCCTCCTGTAACGGCTCA 6 0.15 No Hit TGATGATTGCTCCCATGGTGATGGGCCACAAGAAGAGAAGAAGGTTTTGT 6 0.15 No Hit CCAAGATGACGGTTATCGTGATTATTTATATTTCAGATCTGAATCTGTGT 6 0.15 No Hit GATGGCTTGAGTGGAAAGTTCCAGTTGGTTAAGGGTGAGATGGAACCTTC 6 0.15 No Hit CACCATCAAAATAGCTGATGACCCCGAAACATACAATCGAGTTTTAATCT 6 0.15 No Hit GAGGAGTTCCGTCTGGCTCAGATATGTGGTCTTAACATCATTATACAGGT 6 0.15 No Hit CTCGGGATGATGATGATCTGGTGGAGTCTTTGGAAAGAGATGTTAGTGAG 6 0.15 No Hit AGGAAACAACACAAGTAAGAACCGAAAAGAATTTCACACGACACCAGAGT 5 0.125 No Hit ATTTGATGTGGAATAATGCGATCAGTAGTGAACATTATTTTGTTAAACAA 5 0.125 No Hit CTTCGAACTCCCTCAGGAAGAGAAAGAGGTGTGTGCTAGGCCTCGTGATT 5 0.125 No Hit GACCTGTCGAATGGTTGAAACCTTATACTGATGAGACAATCGTTGAGCTT 5 0.125 No Hit CACAGTGCCATCTGATTGGATGTGCACTATCTGTGGTTGTGTCAATTTTG 5 0.125 No Hit CTCAGCTATAACAAAAGGAGAGACACCAAGGCTTAAGTTATTAGAGCAAA 5 0.125 No Hit CTGATTTCTGTTCTGTTGCTCTCTCACTCTCTTTCCCATGGAAACCTTAC 5 0.125 No Hit CAGATGTTCTCGTCAACACTAATGGCAGAAACAGCATATGGCATTGTCCC 5 0.125 No Hit CAGCAGTGGTTGGGAACAATGTCTCCATTTTGCATCATGTTACGCTTGGA 5 0.125 No Hit GCAGTGCAGAAATCATACCATTTTTGAAAACTTGGGTGAATTTGCCTATG 5 0.125 No Hit GATCTATCGGTTCATCTCCGGAGTCCACAAGGAGATCATGACTTTTGAGT 5 0.125 No Hit GTAAAACTTTGTGTGTATCACACTCCCATGATCATGTACATTAAAGCTGA 5 0.125 No Hit GTTCGAAGAGAATTTCTGCTTTTGTCAGTGTGTTTATCCTACCATAATGG 5 0.125 No Hit CAGGGCTTCAGGTCACTGCCAGAACAGCTGGGGCTGCAAAGACAATTGAG 5 0.125 No Hit CCAGTTTTGATGAAGCACCTTGGCTTGCTTTATCTCTAGAAGCAAAAGAC 5 0.125 No Hit CTTGAAGGAAGGTGCGACAGATCTTGTTTACCGGTGGCAAGAAGTTGCTA 5 0.125 No Hit GTTTCCTTAGACCCGAACATGGCTGCAACGACTGCTGTTGCCGCGTCCTA 5 0.125 No Hit CTCTAGTTTCTCCATAGATCTACTCTTCATGGATCATCCTTCGATCTTTT 5 0.125 No Hit CTCCCCGCCACCAAAACCACCCGATCCCTGCCGTCATGGTAACCTAACCT 5 0.125 No Hit GCTAAATATTACCATCAATTCCCTGCAAGCTGAGAGGAAGAAGCTTCAAG 5 0.125 No Hit CCTGAAGAACCAGCACTTCCTGTAACTTCCACAACTAACAACAACAACAA 5 0.125 No Hit GGGACTTGTTGATAGAGATGCAGAAAGTGGTTGGGTGAAAGGCCAGCCCC 5 0.125 No Hit AGAAGCAGAAAGGAAAATAGATGAGTTGAGGACCAAAATAGAATCAGCTG 5 0.125 No Hit GTTTTACGTGAGCAGGTTCTTGAACCCTTTTTGCTTGCACTTCTCACACC 5 0.125 No Hit AGTTTTTCTTGTACCAGCTTCTTCGAGCTCTCAAATATATACATGCAGGA 5 0.125 No Hit TGTATTTGTTTGTTGAAAATGACTGGGGGTTTGAATTTTATTCTCCTTCT 5 0.125 No Hit AGCGCCAGTTGAATTTCCAAGCTGCAGCGTTGGTGTTTCTGAAGGAGGAG 5 0.125 No Hit GGAGATGAGAAGGGAGGCCGAAGGACAGTAGAAGGGGGTAGAAAACCGAG 5 0.125 No Hit CCTGAGTATTACACGGACGATATCATCAAAGGGCTTGTGGTGGTGATCTT 5 0.125 No Hit GTCCGGGTCAGGAGGTCCAAACAAGCCAATACTCAACCTGGAAAACCCAA 5 0.125 No Hit GCAAGGATCAAGATAATTTGTCTTCTCGTGCATCAAGGTCATCTGCTAAC 5 0.125 No Hit GGCTGTTATCTGCTCCACTTTCGCAGCTGCTACAGTTTTCAGCTCTTCCT 5 0.125 No Hit GTCAACACTTTTAACAATGTTAACGGGCACACATTCAGAAGCTGCATCAT 5 0.125 No Hit GCAGAGGGGGAGAGAGAGAGAGAATAAAGACAATATGTGAGATTTCTTTC 5 0.125 No Hit GAGCAAATCATTGCTAAGGATAATGATGATGAAAAGGATCAGATATTTTC 5 0.125 No Hit TCCCTCTCAAGATGCTTCTCTACTGTTGTAGATGGGTTGAAGTATGCATC 5 0.125 No Hit TTCAAGAGTAAGACAGAATAATTTGTTCTTGTCTAACAAATGGGTTCTGT 5 0.125 No Hit GTTTAGATGTTGCAACTCAATGGAATTCAACTTATCTTATGCTAGAAGCT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0625 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.25 0.0 0.0 0.0 0.0 80-81 0.375 0.0 0.0 0.0 0.0 82-83 0.42500000000000004 0.0 0.0 0.0 0.0 84-85 0.5 0.0 0.0 0.0 0.0 86-87 0.575 0.0 0.0 0.0 0.0 88-89 0.65 0.0 0.0 0.0 0.0 90-91 0.7125 0.0 0.0 0.0 0.0 92-93 0.8875 0.0 0.0 0.0 0.0 94-95 1.0625 0.0 0.0 0.0 0.0 96-97 1.1124999999999998 0.0 0.0 0.0 0.0 98-99 1.275 0.0 0.0 0.0 0.0 100-101 1.525 0.0 0.0 0.0 0.0 102-103 1.8625 0.0 0.0 0.0 0.0 104-105 2.15 0.0 0.0 0.0 0.0 106-107 2.4 0.0 0.0 0.0 0.0 108-109 2.6875 0.0 0.0 0.0 0.0 110-111 3.1 0.0 0.0 0.0 0.0 112-113 3.625 0.0 0.0 0.0 0.0 114-115 3.9000000000000004 0.0 0.0 0.0 0.0 116-117 4.237500000000001 0.0 0.0 0.0 0.0 118-119 4.75 0.0 0.0 0.0 0.0 120-121 5.2 0.0 0.0 0.0 0.0 122-123 5.824999999999999 0.0 0.0 0.0 0.0 124-125 6.2875 0.0 0.0 0.0 0.0 126-127 6.7 0.0 0.0 0.0 0.0 128-129 7.300000000000001 0.0 0.0 0.0 0.0 130-131 7.8375 0.0 0.0 0.0 0.0 132-133 8.375 0.0 0.0 0.0 0.0 134-135 8.8625 0.0 0.0 0.0 0.0 136-137 9.6625 0.0 0.0 0.0 0.0 138-139 10.425 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAAGAGA 10 0.006830828 145.0 4 ACTAAGT 10 0.006830828 145.0 1 TAAGTGG 10 0.006830828 145.0 3 >>END_MODULE Read 913398 spots for SRR13695433.sra Written 913398 spots for SRR13695433.sra Read 913398 spots for SRR13695433.sra Written 913398 spots for SRR13695433.sra Read 913398 spots for SRR13695433.sra Written 913398 spots for SRR13695433.sra Read 913398 spots for SRR13695433.sra Written 913398 spots for SRR13695433.sra Read 913398 spots for SRR13695433.sra Written 913398 spots for SRR13695433.sra Read 913398 spots for SRR13695433.sra Written 913398 spots for SRR13695433.sra Read 913398 spots for SRR13695433.sra Written 913398 spots for SRR13695433.sra Read 913398 spots for SRR13695433.sra Written 913398 spots for SRR13695433.sra Read 913398 spots for SRR13695433.sra Written 913398 spots for SRR13695433.sra Read 913398 spots for SRR13695433.sra Written 913398 spots for SRR13695433.sra Read 913398 spots for SRR13695433.sra Written 913398 spots for SRR13695433.sra Read 913398 spots for SRR13695433.sra Written 913398 spots for SRR13695433.sra Read 913398 spots for SRR13695433.sra Written 913398 spots for SRR13695433.sra Read 913398 spots for SRR13695433.sra Written 913398 spots for SRR13695433.sra Read 913398 spots for SRR13695433.sra Written 913398 spots for SRR13695433.sra Read 913398 spots for SRR13695433.sra Written 913398 spots for SRR13695433.sra Read 913398 spots for SRR13695433.sra Written 913398 spots for SRR13695433.sra Read 913398 spots for SRR13695433.sra Written 913398 spots for SRR13695433.sra Read 913398 spots for SRR13695433.sra Written 913398 spots for SRR13695433.sra Read 913402 spots for SRR13695433.sra Written 913402 spots for SRR13695433.sra SRR ids: ['SRR13695433.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_u7mijoaz SRR13695433.sra spots: 18267964 blocks: [[1, 913398], [913399, 1826796], [1826797, 2740194], [2740195, 3653592], [3653593, 4566990], [4566991, 5480388], [5480389, 6393786], [6393787, 7307184], [7307185, 8220582], [8220583, 9133980], [9133981, 10047378], [10047379, 10960776], [10960777, 11874174], [11874175, 12787572], [12787573, 13700970], [13700971, 14614368], [14614369, 15527766], [15527767, 16441164], [16441165, 17354562], [17354563, 18267964]] SRR13695433 file size 6186553 SRR13695433 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695433 SRR13695433_1.fastq SRR13695433_2.fastq Input file: SRR13695433_1.fastq Paired file: SRR13695433_2.fastq trimmed: SRR13695433-trimmed-pair1.fastq, SRR13695433-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 02:12:28 2025 >> started Wed Feb 12 02:12:49 2025 >> done (20.977s) 18267964 read pairs processed; of these: 147 ( 0.00%) short read pairs filtered out after trimming by size control 1804 ( 0.01%) empty read pairs filtered out after trimming by size control 18266013 (99.99%) read pairs available; of these: 2528136 (13.84%) trimmed read pairs available after processing 15737877 (86.16%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 3 0.00% 20 5 0.00% 21 2 0.00% 22 4 0.00% 23 4 0.00% 24 5 0.00% 25 3 0.00% 26 5 0.00% 27 5 0.00% 28 5 0.00% 29 8 0.00% 30 5 0.00% 31 6 0.00% 32 5 0.00% 33 6 0.00% 34 16 0.00% 35 3 0.00% 36 7 0.00% 37 9 0.00% 38 7 0.00% 39 3 0.00% 40 21 0.00% 41 9 0.00% 42 24 0.00% 43 18 0.00% 44 20 0.00% 45 20 0.00% 46 34 0.00% 47 26 0.00% 48 41 0.00% 49 64 0.00% 50 52 0.00% 51 71 0.00% 52 81 0.00% 53 62 0.00% 54 90 0.00% 55 107 0.00% 56 184 0.00% 57 208 0.00% 58 224 0.00% 59 257 0.00% 60 391 0.00% 61 390 0.00% 62 488 0.00% 63 532 0.00% 64 586 0.00% 65 682 0.00% 66 806 0.00% 67 982 0.01% 68 1068 0.01% 69 1313 0.01% 70 1543 0.01% 71 1826 0.01% 72 2034 0.01% 73 2362 0.01% 74 2783 0.02% 75 3025 0.02% 76 3298 0.02% 77 3767 0.02% 78 4136 0.02% 79 4667 0.03% 80 5298 0.03% 81 5872 0.03% 82 6475 0.04% 83 6962 0.04% 84 7530 0.04% 85 8231 0.05% 86 9002 0.05% 87 9532 0.05% 88 10333 0.06% 89 10710 0.06% 90 11818 0.06% 91 12428 0.07% 92 12975 0.07% 93 14086 0.08% 94 15081 0.08% 95 15865 0.09% 96 16780 0.09% 97 17090 0.09% 98 18103 0.10% 99 18710 0.10% 100 20391 0.11% 101 20819 0.11% 102 21761 0.12% 103 23065 0.13% 104 24264 0.13% 105 24694 0.14% 106 25925 0.14% 107 26922 0.15% 108 27694 0.15% 109 28420 0.16% 110 29081 0.16% 111 30687 0.17% 112 32241 0.18% 113 32158 0.18% 114 33892 0.19% 115 34531 0.19% 116 35752 0.20% 117 37071 0.20% 118 37741 0.21% 119 38484 0.21% 120 39388 0.22% 121 42069 0.23% 122 42070 0.23% 123 43590 0.24% 124 44568 0.24% 125 44728 0.24% 126 46692 0.26% 127 46915 0.26% 128 47818 0.26% 129 48587 0.27% 130 49912 0.27% 131 50292 0.28% 132 51413 0.28% 133 53065 0.29% 134 53432 0.29% 135 54028 0.30% 136 54463 0.30% 137 55614 0.30% 138 56077 0.31% 139 58464 0.32% 140 58654 0.32% 141 58440 0.32% 142 60554 0.33% 143 61203 0.34% 144 62419 0.34% 145 62841 0.34% 146 62805 0.34% 147 64565 0.35% 148 65305 0.36% 149 65635 0.36% 150 65647 0.36% 151 15737877 86.16% 18266013 reads passed initial QC criterion=sequence-density sequence-density=0.40 sequence-density-rank=1 fanout-score=1.96 fanout-score-rank=26 prefix-density=0.40 prefix-fanout=1.9 sequence=CGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTTCGAGCGAAGAAGGT criterion=fanout-score sequence-density=0.01 sequence-density-rank=28 fanout-score=351.82 fanout-score-rank=1 prefix-density=0.26 prefix-fanout=18.0 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAG criterion=sequence-density sequence-density=1.04 sequence-density-rank=1 fanout-score=2.02 fanout-score-rank=28 prefix-density=1.03 prefix-fanout=2.0 sequence=CTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAGTGAACAAACAAAAGTGTCCGTGAGTGTCTTTATCTTTGTGTCTTCTTCA criterion=fanout-score sequence-density=0.01 sequence-density-rank=32 fanout-score=41.78 fanout-score-rank=1 prefix-density=0.05 prefix-fanout=6.4 sequence=AAAGGATGGTAGAGTAGCATCCTTAGCGTTGCTGGTTTACTTTCCTAACAATCCTCAAAACCATTCTTCTTAGACTCTCTATACATTCCAAATAACCAAATTTGTACTGTATAGATATATAGTCTACGTCAAGCTTAAATAAATCCTCATTAACATGGCCCCAGGAGTGCCTATAGATGGGAATATTTTGGGTACCGGGAAGGTTTCCACAGTTAACACTGGCTATTCTAAGAGGGCCTACGTGACATTTTTAGCCGGCAACGGGGATTATGTTAAAGGGGTAGTTGGGTTGGCTAAGGGTTTGCGCAAGGTGAAGAGTGCATACCCTCTTGTCGTAGCAATCTTGCCGGATGTGCCCGAGGAACACCGTGACATTTTGAGGTCTCAAGGTTGCATTGTTCGTGAGATCGAGCCTATTTATCCACCTGAGAACCAGATTCAGTTTGCCATGGCCTACTACGTGATCAACTACTCCAAGCTCCGAATTTGGAATTTTGAGGAGTACAGCAAG SRR13695433 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 02:13:31 Started mapping on | Feb 12 02:13:31 Finished on | Feb 12 02:15:22 Mapping speed, Million of reads per hour | 592.41 Number of input reads | 18266013 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 17213070 Uniquely mapped reads % | 94.24% Average mapped length | 293.33 Number of splices: Total | 16535473 Number of splices: Annotated (sjdb) | 16172796 Number of splices: GT/AG | 16196473 Number of splices: GC/AG | 266831 Number of splices: AT/AC | 9962 Number of splices: Non-canonical | 62207 Mismatch rate per base, % | 0.37% Deletion rate per base | 0.03% Deletion average length | 2.86 Insertion rate per base | 0.02% Insertion average length | 2.32 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 409573 % of reads mapped to multiple loci | 2.24% Number of reads mapped to too many loci | 87727 % of reads mapped to too many loci | 0.48% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.89% % of reads unmapped: other | 0.15% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 643598 643598 643598 N_multimapping 409573 409573 409573 N_noFeature 718264 16691898 1081024 N_ambiguous 272299 2188 111993 UnstrandedReadsAssigned:16222507 PositiveStrandReadsAssigned:518984 NegativeStrandReadsAssigned:16020053 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695433 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695433-trimmed-pair1.fastq SRR13695433-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 18,266,013 reads, 16,064,131 reads pseudoaligned [quant] estimated average fragment length: 241.614 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,038 rounds 52401 SRR13695433.ke.tsv 34699 SRR13695433.se.tsv 87100 total ==> SRR13695433.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1777.39 700 23.0833 Potri.005G024800.1.v4.1 1035 794.386 329 24.2743 Potri.004G059700.1.v4.1 961 720.477 2 0.162701 Potri.007G009000.2.v4.1 1416 1175.39 0 0 Potri.003G141000.2.v4.1 2943 2702.39 1144.02 24.8122 Potri.016G087400.1.v4.1 270 89.4883 640 419.175 Potri.015G069301.1.v4.1 564 332.354 0 0 Potri.010G195200.1.v4.1 1773 1532.39 45 1.72118 Potri.012G127500.1.v4.1 977 736.452 47 3.74055 ==> SRR13695433.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 69 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 254 Potri.001G212900.v4.1 6 Potri.001G182400.v4.1 1 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 24 Potri.001G416900.v4.1 2 Potri.001G452600.v4.1 0 SRR13695433 completed mapping pipeline successfully