Starting /dee2/code/volunteer_pipeline.sh SRR13695434 current disk space = 3048904441856 free memory = 1576379456 SRR13695434 SRAfilesize 73edf60406335f7d560718609d55f8ac SRR13695434.sra SRR13695434.sra file validated SRR13695434 is paired end SRR13695434 is conventional basespace SRR13695434 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695434_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.6555 37.0 37.0 37.0 37.0 37.0 2 36.26025 37.0 37.0 37.0 37.0 37.0 3 36.5955 37.0 37.0 37.0 37.0 37.0 4 36.598 37.0 37.0 37.0 37.0 37.0 5 36.6015 37.0 37.0 37.0 37.0 37.0 6 36.5745 37.0 37.0 37.0 37.0 37.0 7 36.533 37.0 37.0 37.0 37.0 37.0 8 36.5305 37.0 37.0 37.0 37.0 37.0 9 36.594 37.0 37.0 37.0 37.0 37.0 10-14 36.5586 37.0 37.0 37.0 37.0 37.0 15-19 36.5794 37.0 37.0 37.0 37.0 37.0 20-24 36.5132 37.0 37.0 37.0 37.0 37.0 25-29 36.4636 37.0 37.0 37.0 37.0 37.0 30-34 36.465500000000006 37.0 37.0 37.0 37.0 37.0 35-39 36.4437 37.0 37.0 37.0 37.0 37.0 40-44 36.4679 37.0 37.0 37.0 37.0 37.0 45-49 36.411899999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.3966 37.0 37.0 37.0 37.0 37.0 55-59 36.3729 37.0 37.0 37.0 37.0 37.0 60-64 36.3648 37.0 37.0 37.0 37.0 37.0 65-69 36.339800000000004 37.0 37.0 37.0 37.0 37.0 70-74 36.3322 37.0 37.0 37.0 37.0 37.0 75-79 36.334900000000005 37.0 37.0 37.0 37.0 37.0 80-84 36.234 37.0 37.0 37.0 37.0 37.0 85-89 36.2779 37.0 37.0 37.0 37.0 37.0 90-94 36.2118 37.0 37.0 37.0 37.0 37.0 95-99 36.1597 37.0 37.0 37.0 37.0 37.0 100-104 36.151799999999994 37.0 37.0 37.0 37.0 37.0 105-109 36.178 37.0 37.0 37.0 37.0 37.0 110-114 36.0957 37.0 37.0 37.0 37.0 37.0 115-119 36.05069999999999 37.0 37.0 37.0 37.0 37.0 120-124 36.012299999999996 37.0 37.0 37.0 37.0 37.0 125-129 35.95 37.0 37.0 37.0 37.0 37.0 130-134 35.963 37.0 37.0 37.0 37.0 37.0 135-139 35.899899999999995 37.0 37.0 37.0 37.0 37.0 140-144 35.8007 37.0 37.0 37.0 37.0 37.0 145-149 35.6745 37.0 37.0 37.0 37.0 37.0 150-151 35.416250000000005 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 1.0 24 0.0 25 1.0 26 6.0 27 7.0 28 14.0 29 19.0 30 24.0 31 34.0 32 43.0 33 75.0 34 133.0 35 340.0 36 2947.0 37 356.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 46.35 13.175 6.2 34.275 2 20.32152725445868 13.840743531775937 36.27229339361969 29.56543582014569 3 16.75 18.15 29.2 35.9 4 21.825 24.5 24.375 29.299999999999997 5 24.825 31.125000000000004 22.8 21.25 6 20.075000000000003 34.65 23.525 21.75 7 14.099999999999998 27.925 41.25 16.725 8 17.4 25.95 32.9 23.75 9 18.15 24.45 33.275 24.125 10-14 19.345000000000002 30.294999999999998 27.665 22.695 15-19 20.115 27.715 28.765 23.405 20-24 20.54 28.865000000000002 27.589999999999996 23.005 25-29 19.939999999999998 28.57 27.145000000000003 24.345 30-34 19.84 29.48 27.055 23.625 35-39 20.355 28.410000000000004 27.825 23.41 40-44 20.145 29.515 27.13 23.21 45-49 20.515 28.215 27.939999999999998 23.330000000000002 50-54 19.915 27.925 28.655 23.505000000000003 55-59 19.52 29.03 27.794999999999998 23.655 60-64 20.28 28.904999999999998 27.310000000000002 23.505000000000003 65-69 19.88 28.965000000000003 27.200000000000003 23.955000000000002 70-74 20.055 28.389999999999997 28.050000000000004 23.505000000000003 75-79 19.845 28.904999999999998 28.16 23.09 80-84 20.755000000000003 27.77 27.97 23.505000000000003 85-89 20.69 28.615000000000002 27.785 22.91 90-94 20.315 28.58 27.584999999999997 23.52 95-99 20.335 29.13 27.765 22.770000000000003 100-104 20.185 29.054999999999996 27.76 23.0 105-109 20.205000000000002 28.360000000000003 27.55 23.885 110-114 20.34 29.015 27.61 23.035 115-119 20.9 28.275 27.58 23.244999999999997 120-124 21.195 29.375 26.1 23.330000000000002 125-129 21.3 28.645 26.105 23.95 130-134 20.47 28.105000000000004 27.345000000000002 24.08 135-139 21.349999999999998 27.855 26.755000000000003 24.04 140-144 20.8 28.189999999999998 26.86 24.15 145-149 21.185000000000002 27.800000000000004 27.065 23.95 150-151 21.6875 26.575 27.212500000000002 24.525 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.5 19 1.0 20 0.5 21 0.0 22 0.5 23 0.5 24 0.5 25 1.5 26 3.5 27 7.5 28 14.5 29 22.5 30 21.5 31 25.5 32 39.0 33 43.5 34 55.0 35 74.5 36 91.0 37 108.5 38 135.0 39 162.5 40 201.5 41 239.0 42 248.0 43 233.5 44 268.0 45 280.0 46 248.0 47 255.0 48 240.0 49 195.5 50 161.0 51 130.5 52 103.5 53 86.0 54 61.5 55 50.0 56 47.5 57 37.0 58 25.5 59 28.5 60 24.0 61 11.0 62 4.5 63 1.5 64 3.5 65 3.0 66 0.5 67 0.5 68 0.5 69 0.5 70 0.0 71 0.0 72 0.0 73 0.5 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.475 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.025 #Duplication Level Percentage of deduplicated Percentage of total 1 69.25407042786823 45.725 2 18.856493752366525 24.9 3 6.626277925028398 13.125 4 3.369935630443014 8.9 5 1.0223400227186672 3.375 6 0.41650889814464215 1.6500000000000001 7 0.2650511170011359 1.225 8 0.07572889057175312 0.4 9 0.07572889057175312 0.44999999999999996 >10 0.03786444528587656 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTCACCTTCCTCTTTCTGGTAGTTGGAAACAACAGTAGGAACCCTTTTGA 10 0.25 No Hit CCAGGCTTCAATGCACTGATAGCTGCTTCATGGGCCTGAAGGAGGATCTC 9 0.22499999999999998 No Hit GTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACC 9 0.22499999999999998 No Hit GCAGCTATGAGATTGGTCATCGCCAAAATCTCAGGGTCATTTTTATATGG 8 0.2 No Hit GTGAACTTCTGTTTTCTTGCTCGTTTCCAGCTGAGCATGATGCTGTTGCC 8 0.2 No Hit GGCAAGACGAAGAAGTAAGACACACGTGCGACCCCATTCGATCATATACC 7 0.17500000000000002 No Hit CATCCATCAAGTTGGCATTTTTAATATGGAGAAAAGATGGACGATTTATG 7 0.17500000000000002 No Hit CACTCATTCCCTTGCATTTTTCCTCCACGCTGGGCTTTCCCTCCATGGAA 7 0.17500000000000002 No Hit GCCCTGTATCAGCTTCTTGGCACACTTGCTCTCCTAATTTACATGGCGGT 7 0.17500000000000002 No Hit TGGATATGACAGTCAGAAAACGGGAAGAAGCAATTTTTTGAACAAAAATT 7 0.17500000000000002 No Hit GTTCGCTGAGACCCTGTCCCGACATTTTGAAGGATATATGGAAGCTCCAA 7 0.17500000000000002 No Hit ATTCCGGGAGAAATTAAGACAACAAACACGAACTTAAACGCAAGGCTGCA 7 0.17500000000000002 No Hit GGTGATCGAGGGACGGAGGAGGAGCGAAGACGACAACATTTCGATCTTGG 6 0.15 No Hit GGCATTTTATTTAAATATCTGTGTTGGTTCACACTGCAGCTGGGATATAG 6 0.15 No Hit CCTCATTTCCTTCAAGCAGCTCTCGTGAATGGTGTGTCCACATGGCAAGA 6 0.15 No Hit GGGAAATAATAATTCATTGATTATAGTTTAAAAAAGGGATCAAAATAACA 6 0.15 No Hit GCCAGTTCCAACATAAAGAGGGCCTCCATAACCATCCGTACCATGGAATT 6 0.15 No Hit GGCCAGCTTGAGCAAGTTAGGGCCTTCCCTCATGATGGCAGCACCCCCAG 6 0.15 No Hit CTCTATCACAAACTATATTGTGATGTAGAACTACAGCACCTGTGGTACTT 6 0.15 No Hit GAGAAAATGACCCAGGTGATTTGTGGCAAATTGCATCTCTATTCCATCTT 6 0.15 No Hit GGTGGAAGTTGCAAAGGAAAGATGAAGAAGATGAAGTGGAGAGGTTTTGT 6 0.15 No Hit GACTCAGCAGAGCATGTCCTTTCACACAATCCATCTCTTTGAGGTGGTCA 6 0.15 No Hit GGAAAAGCCGTTCACTGACAACCCTCCATCAATACAAATGACCTGTCCAG 6 0.15 No Hit GCCCTAGTCAGGCATCAGTTGCCTTATCCTTCTCAGCAGTTTTTCCTTCT 5 0.125 No Hit GCCCACTTCGCTTATTGTTCATTCCGACACGAACAGCCATTTTGGAATTC 5 0.125 No Hit CCACAAATTACACGAGTTTAGCTCACCATATCACATACTATAACCATACA 5 0.125 No Hit GCCCTCTTTATGCTTCTCCAGCGTATCTCGTGTTCTCTGATAAGCACTCT 5 0.125 No Hit TACAAAACAAAACGTTTTTCAACAAGCATCCATCCTACTAAGAGTACATA 5 0.125 No Hit GTGGAACCTTAAAGCACAACATGATACGAGTCAATGGCAGCTTATAATAA 5 0.125 No Hit AGCTGATTTAAGCAAAGTTGCCACCTTATACCAACACCAGGCTCCACGGA 5 0.125 No Hit CAGCTAACAAAGCAATGGCCGATCCAACGGCAGCAACACCACCTTCCTCG 5 0.125 No Hit CAAGAGTGTAAGGCAAGCCCTTTTTATTGGTGGATCTAATCAAATCCAAA 5 0.125 No Hit ATCAAACTCTTTGCTAGGAGGATAATCAGGCAAACTTGAAGGATCACAAG 5 0.125 No Hit GGCCCAAGTTGGGTGCCCAAGCAACATCCCTCACCCAATCAGTATGCATG 5 0.125 No Hit GTCGTCGTCATCGTCATCAAGTAATTGCTTCTTATGATCATGAGGGTACT 5 0.125 No Hit CCTTCATTGTACATATTTTCACAAGCAAAAATTTGCTCCCATTGTTGCTC 5 0.125 No Hit GTCGGAGAGAATAATTGCTGAATATATTAACAAGCACATGTAAATAATTT 5 0.125 No Hit GTAGTATTTTGACAGCCACAAGCACAAGTAGGGCAGCTTACTATTGTGTC 5 0.125 No Hit GGCATAATCAATAGTCATCTCATGTTCCAATTGATATTGCAGACCTTGTA 5 0.125 No Hit ACAAAATAGGACGTTCTGGAGGAGCTTGGCATCCTGTCTCGTCACGGTCC 5 0.125 No Hit CCACCATCCAAGAACACTGGAACTCGTCCTTGTGCAGCCTTGACAACCTC 5 0.125 No Hit CCTGACCTTTTCGGGAACTTTGCTGAAGACCAAAGTCTTCACAATGGGTG 5 0.125 No Hit CCATGATGTATTTGCCATTTCCCATGGAGACAACATCTCTAAGAGGGTTA 5 0.125 No Hit CCCTGCTACTAGAAGTGCGCTGCTGGCTGCATGCTGCATCCAGAAGGGCT 5 0.125 No Hit CTAAAAAATACTATGTAAAGGGCAGTTACCTATACTTGGCCGATCCTGGC 5 0.125 No Hit GAATCACTTATCATGTTGGTACTTCTTTCATTTGCTTCCTGGGTTAGTGC 5 0.125 No Hit CTTGGTTGATCGACTTCAAAAATATCATCCTCCAGAATGGAAAGACCAGT 5 0.125 No Hit GTTGCATCAAGCTTCTTGTTACCATTTGGTGTGCTGGCCCAGACATTGTA 5 0.125 No Hit CATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTC 5 0.125 No Hit GGAAGACAGCTACTGGCATCGGTGTAATAAGGCTTCAACTGAAAGAAATC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.15 0.0 0.0 0.0 0.0 72-73 0.16249999999999998 0.0 0.0 0.0 0.0 74-75 0.21250000000000002 0.0 0.0 0.0 0.0 76-77 0.225 0.0 0.0 0.0 0.0 78-79 0.225 0.0 0.0 0.0 0.0 80-81 0.275 0.0 0.0 0.0 0.0 82-83 0.3875 0.0 0.0 0.0 0.0 84-85 0.4375 0.0 0.0 0.0 0.0 86-87 0.5625 0.0 0.0 0.0 0.0 88-89 0.6125 0.0 0.0 0.0 0.0 90-91 0.6625000000000001 0.0 0.0 0.0 0.0 92-93 0.8625 0.0 0.0 0.0 0.0 94-95 1.0875 0.0 0.0 0.0 0.0 96-97 1.325 0.0 0.0 0.0 0.0 98-99 1.6 0.0 0.0 0.0 0.0 100-101 1.7625 0.0 0.0 0.0 0.0 102-103 2.0875 0.0 0.0 0.0 0.0 104-105 2.275 0.0 0.0 0.0 0.0 106-107 2.625 0.0 0.0 0.0 0.0 108-109 2.7125000000000004 0.0 0.0 0.0 0.0 110-111 2.9875 0.0 0.0 0.0 0.0 112-113 3.4124999999999996 0.0 0.0 0.0 0.0 114-115 3.7125 0.0 0.0 0.0 0.0 116-117 4.125 0.0 0.0 0.0 0.0 118-119 4.725 0.0 0.0 0.0 0.0 120-121 5.025 0.0 0.0 0.0 0.0 122-123 5.55 0.0 0.0 0.0 0.0 124-125 6.25 0.0 0.0 0.0 0.0 126-127 6.8875 0.0 0.0 0.0 0.0 128-129 7.512499999999999 0.0 0.0 0.0 0.0 130-131 8.3125 0.0 0.0 0.0 0.0 132-133 8.8 0.0 0.0 0.0 0.0 134-135 9.5125 0.0 0.0 0.0 0.0 136-137 10.1625 0.0 0.0 0.0 0.0 138-139 10.7125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACAATC 10 0.006830828 145.0 3 TATTTCA 10 0.006830828 145.0 4 TGAACTC 35 0.0035366106 20.714287 115-119 >>END_MODULE SRR13695434 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695434_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.135 37.0 37.0 37.0 37.0 37.0 2 36.1095 37.0 37.0 37.0 37.0 37.0 3 36.068 37.0 37.0 37.0 37.0 37.0 4 36.173 37.0 37.0 37.0 37.0 37.0 5 36.278 37.0 37.0 37.0 37.0 37.0 6 36.362 37.0 37.0 37.0 37.0 37.0 7 36.2035 37.0 37.0 37.0 37.0 37.0 8 36.195 37.0 37.0 37.0 37.0 37.0 9 36.2385 37.0 37.0 37.0 37.0 37.0 10-14 36.229600000000005 37.0 37.0 37.0 37.0 37.0 15-19 36.2076 37.0 37.0 37.0 37.0 37.0 20-24 36.0909 37.0 37.0 37.0 37.0 37.0 25-29 36.06375 37.0 37.0 37.0 37.0 37.0 30-34 35.98965 37.0 37.0 37.0 37.0 37.0 35-39 35.99565 37.0 37.0 37.0 37.0 37.0 40-44 36.028650000000006 37.0 37.0 37.0 37.0 37.0 45-49 35.9989 37.0 37.0 37.0 37.0 37.0 50-54 35.94385 37.0 37.0 37.0 37.0 37.0 55-59 35.987049999999996 37.0 37.0 37.0 37.0 37.0 60-64 35.90845 37.0 37.0 37.0 37.0 37.0 65-69 35.76385 37.0 37.0 37.0 37.0 37.0 70-74 35.85765 37.0 37.0 37.0 37.0 37.0 75-79 35.780350000000006 37.0 37.0 37.0 37.0 37.0 80-84 35.795 37.0 37.0 37.0 37.0 37.0 85-89 35.71565 37.0 37.0 37.0 37.0 37.0 90-94 35.67525 37.0 37.0 37.0 37.0 37.0 95-99 35.704750000000004 37.0 37.0 37.0 37.0 37.0 100-104 35.683049999999994 37.0 37.0 37.0 37.0 37.0 105-109 35.61365 37.0 37.0 37.0 37.0 37.0 110-114 35.481449999999995 37.0 37.0 37.0 37.0 37.0 115-119 35.529650000000004 37.0 37.0 37.0 37.0 37.0 120-124 35.44635 37.0 37.0 37.0 37.0 37.0 125-129 35.4478 37.0 37.0 37.0 37.0 37.0 130-134 35.271550000000005 37.0 37.0 37.0 34.6 37.0 135-139 35.19905 37.0 37.0 37.0 29.8 37.0 140-144 35.08895 37.0 37.0 37.0 32.2 37.0 145-149 34.95535 37.0 37.0 37.0 25.0 37.0 150-151 34.686 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 1.0 13 6.0 14 5.0 15 6.0 16 0.0 17 1.0 18 1.0 19 1.0 20 4.0 21 2.0 22 7.0 23 8.0 24 8.0 25 10.0 26 6.0 27 11.0 28 9.0 29 17.0 30 34.0 31 35.0 32 56.0 33 122.0 34 229.0 35 587.0 36 2641.0 37 191.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.2548921224285 22.930255895634723 9.332664325137983 23.482187656798796 2 27.474999999999998 25.45 30.475 16.6 3 21.65 26.125 33.375 18.85 4 24.275 33.525 22.5 19.7 5 26.875 36.15 21.825 15.15 6 19.575 40.300000000000004 22.175 17.95 7 19.275000000000002 23.425 39.1 18.2 8 21.025 24.875 31.275 22.825 9 22.725 22.975 30.65 23.65 10-14 22.869999999999997 29.695 27.495000000000005 19.939999999999998 15-19 23.125 28.285 27.79 20.8 20-24 22.631315657828914 29.239619809904955 27.2736368184092 20.855427713856926 25-29 21.916437327995997 28.651488616462345 28.416312234175635 21.015761821366024 30-34 23.059988992845348 28.57357282233452 28.258367939160458 20.10807024565968 35-39 22.807544149282105 27.990394717094404 28.125469007954372 21.07659212566912 40-44 22.45459548706659 28.55355981387902 28.37844598989343 20.613398709160954 45-49 22.338403041825096 27.55153091855113 29.052431458875326 21.05763458074845 50-54 21.37675721646906 28.70078543198759 29.161038571214164 20.76141878032918 55-59 22.431823867900928 28.20615461596197 28.696522391793845 20.665499124343256 60-64 21.501826004302366 28.54570013507429 28.780829456200912 21.171644404422434 65-69 22.78297404091432 28.15985594958235 27.794728154854198 21.26244185464913 70-74 23.49762321741306 29.02677007755817 26.870152614460846 20.605454090567925 75-79 23.715415019762844 27.763045979886925 28.183319157452345 20.338219842897885 80-84 23.276638319159577 28.07403701850926 27.19859929964982 21.450725362681343 85-89 23.357518138603954 28.516387290467847 27.860895671753816 20.26519889917438 90-94 23.277802791535347 28.410625844214316 27.905347941367754 20.406223422882587 95-99 23.54795137325529 28.385612086647654 27.5101305718145 20.556305968282558 100-104 22.907180385288967 28.736552414310733 27.410557918438826 20.945709281961474 105-109 23.76282211658744 29.191893920440332 26.36477358018514 20.68051038278709 110-114 22.79253589474211 28.945920256140877 27.910350692881085 20.35119315623593 115-119 24.91868901676257 28.286214660995746 26.64498373780335 20.15011258443833 120-124 24.60345258944208 28.956717538153615 26.880160120090068 19.559669752314235 125-129 24.26198338837186 28.73011107775443 26.94886420494346 20.059041328930252 130-134 24.648486364773582 28.36627470602952 26.8951713785339 20.090067550662997 135-139 24.553415061295972 28.316237177883412 26.880160120090068 20.250187640730548 140-144 25.26389514232828 28.32057631697434 26.534594026714693 19.88093451398269 145-149 25.924443332499376 28.42131598699024 26.419814861145856 19.234425819364525 150-151 26.54490868151113 27.858393795346508 25.69427070302727 19.902426820115085 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 1.0 4 1.0 5 0.0 6 0.5 7 0.5 8 1.0 9 1.0 10 0.5 11 1.0 12 1.0 13 0.5 14 0.5 15 2.0 16 2.5 17 2.0 18 2.0 19 2.0 20 1.0 21 0.5 22 1.5 23 1.5 24 2.5 25 3.5 26 9.5 27 12.0 28 9.0 29 14.5 30 25.0 31 32.0 32 38.0 33 45.5 34 66.5 35 84.0 36 88.5 37 106.5 38 134.5 39 170.0 40 207.0 41 239.5 42 254.5 43 284.5 44 294.0 45 265.5 46 237.5 47 208.5 48 206.0 49 186.0 50 148.0 51 120.5 52 92.0 53 74.5 54 73.0 55 66.0 56 44.0 57 31.0 58 21.5 59 21.0 60 20.5 61 8.5 62 6.0 63 5.5 64 4.0 65 3.5 66 1.5 67 1.0 68 1.0 69 0.0 70 2.5 71 2.5 72 1.0 73 1.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35000000000000003 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.05 25-29 0.075 30-34 0.065 35-39 0.055 40-44 0.065 45-49 0.06 50-54 0.055 55-59 0.075 60-64 0.055 65-69 0.034999999999999996 70-74 0.075 75-79 0.065 80-84 0.05 85-89 0.075 90-94 0.055 95-99 0.055 100-104 0.075 105-109 0.075 110-114 0.055 115-119 0.075 120-124 0.075 125-129 0.06999999999999999 130-134 0.075 135-139 0.075 140-144 0.055 145-149 0.075 150-151 0.075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.8 #Duplication Level Percentage of deduplicated Percentage of total 1 70.17215568862275 46.875 2 18.15119760479042 24.25 3 6.923652694610778 13.875000000000002 4 2.9191616766467066 7.8 5 1.0479041916167664 3.5000000000000004 6 0.33682634730538924 1.35 7 0.2619760479041916 1.225 8 0.07485029940119761 0.4 9 0.037425149700598806 0.22499999999999998 >10 0.07485029940119761 0.5 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 10 0.25 No Hit GTTAGTTGTAGCTTAGTATTATTACTATTATTATACTACCTATCACATCT 10 0.25 No Hit ATTGGAACCTGCAAGAATTAAGGTAAAGCTGAAGGCAGAGAATGTCGATA 9 0.22499999999999998 No Hit AGAGAACACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 8 0.2 No Hit CTAAGAATAACAAAAAGCTCAAGCAATTATACCATAAGGCACTGGCTATA 8 0.2 No Hit CATTAAAGAGGCGAAGAGAGAAGAAAACATAAAAAGAAACAATCAACAGT 7 0.17500000000000002 No Hit GTTGCTTCCATAATATACCAAATAGGCATGATAAGCTTAACATTATCAGC 7 0.17500000000000002 No Hit AAACAGTGGAAATTAGCAGTGAGAACTCAGGGCCTATGAGATCAAAATCA 7 0.17500000000000002 No Hit CAAACATTATCCTATCCAGAATCCACACCTCCCATGAGCTGACACCATTT 7 0.17500000000000002 No Hit CTGGTGTTTCAATTTATGAAAGTGGTGACATTGTGAAATACCTATTTCAG 7 0.17500000000000002 No Hit CCTTTGTAGCAGTGCTCAGTCCTGACTCAAGCTTCTTCCAAATTGAAGAA 7 0.17500000000000002 No Hit AGTTATATGACTTCGTTCAAATAGCCACTTCAGTAGATCTTGGCTGTTTT 7 0.17500000000000002 No Hit GTTTGTATCAACTGTGGTGTGTGCATGGGAAAGTACTTCTGTGAGACTTG 6 0.15 No Hit GAGTGTCATCAAAGATTAGCAATGGACAGATCGTTCTCAGTCTAGACTGT 6 0.15 No Hit GCCCATGTCATTATTGCTGCCAGGAACATGGTTGCTGCAAACGAAGCTAA 6 0.15 No Hit GGAAGGTGAAGTATGTTAGTAATTTTGATTTGTACAAGTCAAAGTATGCT 6 0.15 No Hit GGTCTACCAAGAAGGTTCGGGTGAAGGAGCAAGTGCAAGTTATCGATCAA 6 0.15 No Hit AGAAGAAGGGACTACTCGAGAAGATCAAGGAGAAGTTACCTGGGTACCAC 6 0.15 No Hit GAAAACTTGAAGTTTTCCATGGAATGTGTTTTGATGCTCTGAAGTTGCCA 6 0.15 No Hit GGCTTCTGTTGCGAACACTTTACTACTCTCTCTGCCTCCGCCACTCTGTT 6 0.15 No Hit CTGCAGAGTCTTCAAAATGAAAGTGTTGTGAAGGAGCTTGCTTATCGGAC 6 0.15 No Hit GAATGTATTGGCAAAGAATCCTTGCTAGCATCAGCAAAGAATGGCTTGGC 5 0.125 No Hit CTTTTAGTTCATCCATTTAAGCTTAATCAATCAATCATCATGTCTAGCAC 5 0.125 No Hit GACAACTGCAGGTCCATTTAAAAGGCCATTCTTGAATTTTTCAAATGTAA 5 0.125 No Hit GTTATAGTGATAATTAAGCTTGACATGGACTAGAAGTTGGAAGTATGGTG 5 0.125 No Hit CTCTGTCTGTCTCTCTCCCCCTCCTCTCTGACTGGCTTGTCTCTCTCTCT 5 0.125 No Hit AGAAGACATGTGGATCCATGTGTTTGATTTCCATACTGGAGAACAGATTG 5 0.125 No Hit GGACATTGTATGTTAATGGCCGAGTATTAGCATGTTAATTTTTTTTTCAA 5 0.125 No Hit GTCATAATATGGAAGGAGGGTAATCAGAATGACTGGACCCAGGCTCATGT 5 0.125 No Hit GACAGCTTGGATATCTATCCTATCAGAATATTTAGTGGATGCCATAGAGG 5 0.125 No Hit GGTCGGCCGGTCCGCCTCAGGTGTGCACCGGTCGCCTCGTCCCTTCTACC 5 0.125 No Hit GGGTGATGATGCTGGAGGATGCATTTAGCAGGTGTAGCAAAGGGAAGGGT 5 0.125 No Hit CAAGGTAGAGAGCAGATAAGATAGCAGACACAAATTGTTATTATAGGCTC 5 0.125 No Hit AGTGAAGGCAAATAAAATCAGTTGCAATTGAAGGCGAGGCCTTACCGAGC 5 0.125 No Hit GATAAGACAACTGAGGTTCCTGATCGAGAACAATATAACAAAGCAGATTT 5 0.125 No Hit GGCGGCTGTGCATGCATCAACGTGGTCGTTCATGAGCCAGAAGCTTTAAA 5 0.125 No Hit AATAAAGATAGGAAAAGAAAATGACCCAAGAAAAAAAAACCCTCTCCTCT 5 0.125 No Hit AAAGGACTGAACTCTCCTCGATGATGATTTAGGGTCTGAAAAGAATAATC 5 0.125 No Hit CTTCCCACTATCACAAAAATGTTCCACGCCTGTAGTAGCGGGGAAGTGGT 5 0.125 No Hit CAGTATCTCATGAGCAGGGGCCAGATTTTGGATCGGATGTCCATGGCACT 5 0.125 No Hit GGAGGCGATACAGCATACGGAGCCAACTTTGAAGTGTGTCTCAAGAGTAA 5 0.125 No Hit CTCAAAGTGACTGGGTGGTGTGAGCGCTTTGCAAATAAATAGGCAGTCTT 5 0.125 No Hit AAGGAATTCCCAGCACCAGCTTTAGCTCTTATGGAGACCCTGCTCGCCAT 5 0.125 No Hit AAAGAACCATGAAGGTTGCTGCTGCAGAGGAAACAGCTGTCAAAGAGGCT 5 0.125 No Hit TGAATGATCAGTGAAGAAGATGGAGTTATGTAGTGTCATTAATAATTATG 5 0.125 No Hit CTCATGGTTCACACTTTCCCTGCAGTCCAAATGTCTTTATGCTTATGAGC 5 0.125 No Hit TGGACCTTGGACTGATGGGAATGAGGAAATGGGAGGAAGCACTTACTTTT 5 0.125 No Hit ATTTTTGACATTGAAGAACTTTGAAGGTTTGGACCTTGGAAAAATGGACA 5 0.125 No Hit GCGGCGTGCACAAGAGAAGATTTCAATACTTCGACGAATCTGCTCTCTCA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.15 0.0 0.0 0.0 0.0 72-73 0.16249999999999998 0.0 0.0 0.0 0.0 74-75 0.21250000000000002 0.0 0.0 0.0 0.0 76-77 0.225 0.0 0.0 0.0 0.0 78-79 0.225 0.0 0.0 0.0 0.0 80-81 0.275 0.0 0.0 0.0 0.0 82-83 0.3875 0.0 0.0 0.0 0.0 84-85 0.4375 0.0 0.0 0.0 0.0 86-87 0.5625 0.0 0.0 0.0 0.0 88-89 0.6125 0.0 0.0 0.0 0.0 90-91 0.6625000000000001 0.0 0.0 0.0 0.0 92-93 0.8625 0.0 0.0 0.0 0.0 94-95 1.0875 0.0 0.0 0.0 0.0 96-97 1.325 0.0 0.0 0.0 0.0 98-99 1.6 0.0 0.0 0.0 0.0 100-101 1.7625 0.0 0.0 0.0 0.0 102-103 2.075 0.0 0.0 0.0 0.0 104-105 2.25 0.0 0.0 0.0 0.0 106-107 2.5625 0.0 0.0 0.0 0.0 108-109 2.6375 0.0 0.0 0.0 0.0 110-111 2.9125 0.0 0.0 0.0 0.0 112-113 3.35 0.0 0.0 0.0 0.0 114-115 3.6625 0.0 0.0 0.0 0.0 116-117 4.0875 0.0 0.0 0.0 0.0 118-119 4.7 0.0 0.0 0.0 0.0 120-121 5.0 0.0 0.0 0.0 0.0 122-123 5.525 0.0 0.0 0.0 0.0 124-125 6.225 0.0 0.0 0.0 0.0 126-127 6.875 0.0 0.0 0.0 0.0 128-129 7.487500000000001 0.0 0.0 0.0 0.0 130-131 8.3125 0.0 0.0 0.0 0.0 132-133 8.774999999999999 0.0 0.0 0.0 0.0 134-135 9.475 0.0 0.0 0.0 0.0 136-137 10.1125 0.0 0.0 0.0 0.0 138-139 10.6625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGGGGG 10 0.006830828 145.0 145 CCCTTGC 10 0.006830828 145.0 7 >>END_MODULE Read 993325 spots for SRR13695434.sra Written 993325 spots for SRR13695434.sra Read 993325 spots for SRR13695434.sra Written 993325 spots for SRR13695434.sra Read 993325 spots for SRR13695434.sra Written 993325 spots for SRR13695434.sra Read 993325 spots for SRR13695434.sra Written 993325 spots for SRR13695434.sra Read 993325 spots for SRR13695434.sra Written 993325 spots for SRR13695434.sra Read 993325 spots for SRR13695434.sra Written 993325 spots for SRR13695434.sra Read 993325 spots for SRR13695434.sra Written 993325 spots for SRR13695434.sra Read 993325 spots for SRR13695434.sra Written 993325 spots for SRR13695434.sra Read 993325 spots for SRR13695434.sra Written 993325 spots for SRR13695434.sra Read 993325 spots for SRR13695434.sra Written 993325 spots for SRR13695434.sra Read 993325 spots for SRR13695434.sra Written 993325 spots for SRR13695434.sra Read 993325 spots for SRR13695434.sra Written 993325 spots for SRR13695434.sra Read 993332 spots for SRR13695434.sra Written 993332 spots for SRR13695434.sra Read 993325 spots for SRR13695434.sra Written 993325 spots for SRR13695434.sra Read 993325 spots for SRR13695434.sra Written 993325 spots for SRR13695434.sra Read 993325 spots for SRR13695434.sra Written 993325 spots for SRR13695434.sra Read 993325 spots for SRR13695434.sra Written 993325 spots for SRR13695434.sra Read 993325 spots for SRR13695434.sra Written 993325 spots for SRR13695434.sra Read 993325 spots for SRR13695434.sra Written 993325 spots for SRR13695434.sra Read 993325 spots for SRR13695434.sra Written 993325 spots for SRR13695434.sra SRR ids: ['SRR13695434.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_othdsd00 SRR13695434.sra spots: 19866507 blocks: [[1, 993325], [993326, 1986650], [1986651, 2979975], [2979976, 3973300], [3973301, 4966625], [4966626, 5959950], [5959951, 6953275], [6953276, 7946600], [7946601, 8939925], [8939926, 9933250], [9933251, 10926575], [10926576, 11919900], [11919901, 12913225], [12913226, 13906550], [13906551, 14899875], [14899876, 15893200], [15893201, 16886525], [16886526, 17879850], [17879851, 18873175], [18873176, 19866507]] SRR13695434 file size 6729807 SRR13695434 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695434 SRR13695434_1.fastq SRR13695434_2.fastq Input file: SRR13695434_1.fastq Paired file: SRR13695434_2.fastq trimmed: SRR13695434-trimmed-pair1.fastq, SRR13695434-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 02:59:57 2025 >> started Wed Feb 12 03:00:28 2025 >> done (31.189s) 19866507 read pairs processed; of these: 175 ( 0.00%) short read pairs filtered out after trimming by size control 2867 ( 0.01%) empty read pairs filtered out after trimming by size control 19863465 (99.98%) read pairs available; of these: 2918103 (14.69%) trimmed read pairs available after processing 16945362 (85.31%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 2 0.00% 20 0 0.00% 21 2 0.00% 22 6 0.00% 23 5 0.00% 24 3 0.00% 25 5 0.00% 26 6 0.00% 27 3 0.00% 28 9 0.00% 29 4 0.00% 30 3 0.00% 31 5 0.00% 32 7 0.00% 33 14 0.00% 34 3 0.00% 35 14 0.00% 36 11 0.00% 37 10 0.00% 38 21 0.00% 39 11 0.00% 40 21 0.00% 41 21 0.00% 42 28 0.00% 43 37 0.00% 44 32 0.00% 45 33 0.00% 46 52 0.00% 47 64 0.00% 48 60 0.00% 49 93 0.00% 50 120 0.00% 51 133 0.00% 52 134 0.00% 53 137 0.00% 54 161 0.00% 55 177 0.00% 56 219 0.00% 57 234 0.00% 58 292 0.00% 59 344 0.00% 60 427 0.00% 61 510 0.00% 62 527 0.00% 63 571 0.00% 64 593 0.00% 65 761 0.00% 66 868 0.00% 67 940 0.00% 68 1106 0.01% 69 1302 0.01% 70 1584 0.01% 71 1720 0.01% 72 2178 0.01% 73 2318 0.01% 74 2649 0.01% 75 2807 0.01% 76 3105 0.02% 77 3577 0.02% 78 3808 0.02% 79 4434 0.02% 80 4923 0.02% 81 5664 0.03% 82 6413 0.03% 83 6850 0.03% 84 8162 0.04% 85 8623 0.04% 86 9192 0.05% 87 9966 0.05% 88 10829 0.05% 89 11505 0.06% 90 12588 0.06% 91 13521 0.07% 92 14720 0.07% 93 16453 0.08% 94 17411 0.09% 95 18535 0.09% 96 19693 0.10% 97 20689 0.10% 98 21757 0.11% 99 22457 0.11% 100 23716 0.12% 101 24673 0.12% 102 26358 0.13% 103 27668 0.14% 104 28698 0.14% 105 29902 0.15% 106 31405 0.16% 107 32005 0.16% 108 33556 0.17% 109 34491 0.17% 110 35043 0.18% 111 36617 0.18% 112 37722 0.19% 113 38895 0.20% 114 40412 0.20% 115 41731 0.21% 116 43063 0.22% 117 44644 0.22% 118 45609 0.23% 119 45882 0.23% 120 47248 0.24% 121 48220 0.24% 122 48900 0.25% 123 50927 0.26% 124 51512 0.26% 125 52766 0.27% 126 54464 0.27% 127 55290 0.28% 128 56335 0.28% 129 57543 0.29% 130 58974 0.30% 131 58708 0.30% 132 59394 0.30% 133 60113 0.30% 134 60958 0.31% 135 62150 0.31% 136 62349 0.31% 137 63381 0.32% 138 65359 0.33% 139 67231 0.34% 140 66506 0.33% 141 67407 0.34% 142 67614 0.34% 143 68051 0.34% 144 69847 0.35% 145 70572 0.36% 146 70582 0.36% 147 71870 0.36% 148 74150 0.37% 149 74320 0.37% 150 75000 0.38% 151 16945362 85.31% 19863465 reads passed initial QC criterion=sequence-density sequence-density=0.52 sequence-density-rank=1 fanout-score=2.01 fanout-score-rank=20 prefix-density=0.52 prefix-fanout=2.0 sequence=TGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGG criterion=fanout-score sequence-density=0.02 sequence-density-rank=25 fanout-score=268.55 fanout-score-rank=1 prefix-density=0.27 prefix-fanout=18.6 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAG criterion=sequence-density sequence-density=1.32 sequence-density-rank=1 fanout-score=2.02 fanout-score-rank=27 prefix-density=1.31 prefix-fanout=2.0 sequence=CTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAGTGAACAAACAAAAGTGTCCGTGAGTGTCTTTATCTTTGTGTCTTCTTCA criterion=fanout-score sequence-density=0.13 sequence-density-rank=27 fanout-score=35.12 fanout-score-rank=1 prefix-density=0.35 prefix-fanout=12.6 sequence=AAAGAAAAGAAAA SRR13695434 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 03:01:05 Started mapping on | Feb 12 03:01:05 Finished on | Feb 12 03:03:15 Mapping speed, Million of reads per hour | 550.07 Number of input reads | 19863465 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 18510605 Uniquely mapped reads % | 93.19% Average mapped length | 292.83 Number of splices: Total | 17901388 Number of splices: Annotated (sjdb) | 17450181 Number of splices: GT/AG | 17529233 Number of splices: GC/AG | 273791 Number of splices: AT/AC | 11456 Number of splices: Non-canonical | 86908 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.03% Deletion average length | 2.89 Insertion rate per base | 0.02% Insertion average length | 2.29 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 444680 % of reads mapped to multiple loci | 2.24% Number of reads mapped to too many loci | 139847 % of reads mapped to too many loci | 0.70% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.65% % of reads unmapped: other | 0.22% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 908419 908419 908419 N_multimapping 444680 444680 444680 N_noFeature 834374 17933674 1217027 N_ambiguous 308966 2531 112686 UnstrandedReadsAssigned:17367265 PositiveStrandReadsAssigned:574400 NegativeStrandReadsAssigned:17180892 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695434 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695434-trimmed-pair1.fastq SRR13695434-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 19,863,465 reads, 17,264,614 reads pseudoaligned [quant] estimated average fragment length: 238.464 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,282 rounds 52401 SRR13695434.ke.tsv 34699 SRR13695434.se.tsv 87100 total ==> SRR13695434.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1780.54 542 15.4898 Potri.005G024800.1.v4.1 1035 797.536 386 24.6282 Potri.004G059700.1.v4.1 961 723.631 2 0.14064 Potri.007G009000.2.v4.1 1416 1178.54 0 0 Potri.003G141000.2.v4.1 2943 2705.54 1451.64 27.3025 Potri.016G087400.1.v4.1 270 90.5551 800 449.545 Potri.015G069301.1.v4.1 564 334.627 0 0 Potri.010G195200.1.v4.1 1773 1535.54 105 3.47957 Potri.012G127500.1.v4.1 977 739.618 78 5.36641 ==> SRR13695434.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 65 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 240 Potri.001G212900.v4.1 3 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 19 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR13695434 completed mapping pipeline successfully