Starting /dee2/code/volunteer_pipeline.sh SRR13695437 current disk space = 3048895639552 free memory = 1575341592 SRR13695437 SRAfilesize bcf695570a3d1c88f2d270c5ed2b61ba SRR13695437.sra SRR13695437.sra file validated SRR13695437 is paired end SRR13695437 is conventional basespace SRR13695437 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695437_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5585 37.0 37.0 37.0 37.0 37.0 2 36.4135 37.0 37.0 37.0 37.0 37.0 3 36.648 37.0 37.0 37.0 37.0 37.0 4 36.6085 37.0 37.0 37.0 37.0 37.0 5 36.6935 37.0 37.0 37.0 37.0 37.0 6 36.6545 37.0 37.0 37.0 37.0 37.0 7 36.584 37.0 37.0 37.0 37.0 37.0 8 36.621 37.0 37.0 37.0 37.0 37.0 9 36.545 37.0 37.0 37.0 37.0 37.0 10-14 36.5911 37.0 37.0 37.0 37.0 37.0 15-19 36.569599999999994 37.0 37.0 37.0 37.0 37.0 20-24 36.5851 37.0 37.0 37.0 37.0 37.0 25-29 36.4877 37.0 37.0 37.0 37.0 37.0 30-34 36.4498 37.0 37.0 37.0 37.0 37.0 35-39 36.4639 37.0 37.0 37.0 37.0 37.0 40-44 36.5 37.0 37.0 37.0 37.0 37.0 45-49 36.403299999999994 37.0 37.0 37.0 37.0 37.0 50-54 36.378499999999995 37.0 37.0 37.0 37.0 37.0 55-59 36.442499999999995 37.0 37.0 37.0 37.0 37.0 60-64 36.3433 37.0 37.0 37.0 37.0 37.0 65-69 36.2727 37.0 37.0 37.0 37.0 37.0 70-74 36.3542 37.0 37.0 37.0 37.0 37.0 75-79 36.3266 37.0 37.0 37.0 37.0 37.0 80-84 36.2937 37.0 37.0 37.0 37.0 37.0 85-89 36.2655 37.0 37.0 37.0 37.0 37.0 90-94 36.175200000000004 37.0 37.0 37.0 37.0 37.0 95-99 36.124 37.0 37.0 37.0 37.0 37.0 100-104 36.119899999999994 37.0 37.0 37.0 37.0 37.0 105-109 36.147499999999994 37.0 37.0 37.0 37.0 37.0 110-114 36.09740000000001 37.0 37.0 37.0 37.0 37.0 115-119 36.091699999999996 37.0 37.0 37.0 37.0 37.0 120-124 36.0372 37.0 37.0 37.0 37.0 37.0 125-129 36.0326 37.0 37.0 37.0 37.0 37.0 130-134 36.05649999999999 37.0 37.0 37.0 37.0 37.0 135-139 35.9014 37.0 37.0 37.0 37.0 37.0 140-144 35.8918 37.0 37.0 37.0 37.0 37.0 145-149 35.7515 37.0 37.0 37.0 37.0 37.0 150-151 35.641999999999996 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 1.0 21 1.0 22 1.0 23 2.0 24 1.0 25 0.0 26 4.0 27 5.0 28 12.0 29 15.0 30 23.0 31 24.0 32 49.0 33 77.0 34 123.0 35 329.0 36 2954.0 37 378.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.25 10.549999999999999 9.275 43.925 2 20.27094831911691 14.425489212242852 36.4274962368289 28.876066231811336 3 20.0 17.025000000000002 25.75 37.225 4 22.85 26.55 23.1 27.500000000000004 5 23.75 30.75 24.95 20.549999999999997 6 19.400000000000002 34.9 24.85 20.849999999999998 7 17.325 23.75 42.025 16.900000000000002 8 18.025 24.55 33.650000000000006 23.775 9 17.45 24.825 35.525 22.2 10-14 20.445 29.465000000000003 27.455000000000002 22.634999999999998 15-19 19.445 27.089999999999996 28.67 24.795 20-24 19.91 28.285 28.134999999999998 23.669999999999998 25-29 19.425 28.465 28.15 23.96 30-34 19.835 28.52 27.834999999999997 23.810000000000002 35-39 20.150000000000002 28.355000000000004 28.199999999999996 23.294999999999998 40-44 19.295 29.044999999999998 27.875 23.785 45-49 19.665 28.655 27.775 23.905 50-54 19.895 27.650000000000002 28.804999999999996 23.65 55-59 20.235 28.544999999999998 28.165000000000003 23.055 60-64 20.200000000000003 28.09 27.99 23.72 65-69 19.39 28.49 28.265 23.855 70-74 20.65 29.555 27.255000000000003 22.54 75-79 20.424999999999997 27.77 28.04 23.765 80-84 20.02 28.345 28.13 23.505000000000003 85-89 20.635 28.535 27.92 22.91 90-94 20.169999999999998 27.889999999999997 28.060000000000002 23.880000000000003 95-99 20.07 28.499999999999996 28.405 23.025000000000002 100-104 21.035 28.005000000000003 28.465 22.495 105-109 20.76 28.525 27.46 23.255 110-114 20.155 28.294999999999998 28.075 23.474999999999998 115-119 20.974999999999998 28.15 27.35 23.525 120-124 20.0 29.225 26.26 24.515 125-129 21.45 28.485 26.484999999999996 23.580000000000002 130-134 20.66 27.99 27.755000000000003 23.595 135-139 20.794999999999998 28.360000000000003 27.055 23.79 140-144 21.135 29.049999999999997 26.640000000000004 23.175 145-149 21.37 28.24 26.76 23.630000000000003 150-151 21.2625 29.075 27.037499999999998 22.625 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 1.0 16 0.5 17 0.5 18 0.5 19 0.5 20 1.5 21 1.0 22 2.5 23 3.5 24 4.5 25 6.5 26 7.0 27 8.0 28 9.5 29 17.5 30 18.0 31 19.0 32 42.0 33 52.5 34 61.5 35 77.0 36 87.0 37 102.5 38 122.5 39 158.5 40 185.5 41 209.0 42 238.5 43 258.5 44 281.5 45 279.5 46 261.5 47 247.5 48 222.5 49 193.0 50 158.5 51 131.0 52 115.0 53 97.5 54 83.0 55 60.0 56 38.0 57 35.5 58 28.0 59 23.5 60 19.5 61 10.0 62 5.0 63 3.0 64 3.5 65 3.5 66 2.0 67 0.5 68 0.5 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.35000000000000003 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.7 #Duplication Level Percentage of deduplicated Percentage of total 1 68.02848575712144 45.375 2 20.76461769115442 27.700000000000003 3 6.5592203898050965 13.125 4 3.2983508245877062 8.799999999999999 5 0.8995502248875562 3.0 6 0.26236881559220393 1.05 7 0.07496251874062969 0.35000000000000003 8 0.11244377811094453 0.6 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGACACCGAGAAGCTTGAGTGCGCTCTCCACTGACATCTCAAACGGCGC 8 0.2 No Hit GGTGAGGTTCAGGAGCTGCGAAGAGCAGAGGGAGGCAGGGAGGCTTGCGT 8 0.2 No Hit TCATCACCAAGCTTGCCCATAAAAGCTGATGAACCACCTAGTCTTGATAT 8 0.2 No Hit CCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGA 7 0.17500000000000002 No Hit TTGGAATAGCGCTTGTCTGCTGCTGCTCCCATAACAAGCTTCCTGATCCC 7 0.17500000000000002 No Hit CCTCAATACAGACACTAGTAAGGGCATGCATGTTCTGTGATGTTGGATAT 6 0.15 No Hit GTACTTTTGGAACTCTTGCCATGTCCAATCAGCAAATTGATTAAGACCAA 6 0.15 No Hit CTCTTCTCCACTTGTTTCTTCAACTTGGCCCTTTCTCTTCTCTCCCTCCT 6 0.15 No Hit GTCAATGTCGAATACCTTGGCCCTCGCCATGGCATCTGCTAAGTTCATGT 6 0.15 No Hit GGAGCAAAGACGAGTGATTCAACAGCCATCAGTTTGATGAGGATGTTAAG 6 0.15 No Hit GTACCTTGGGTCATCAGGATGTATAGCAACAGCCGTATCACCCAGCATAG 6 0.15 No Hit TTCACACCAATGGTTACAATGAGTAGGAGAGGACTGGAAAGGGGTTCAGA 6 0.15 No Hit CTTCTTTCAATCTTGCTCCAGTGGATAGTGCCTGAGATGCACGCTCACTT 5 0.125 No Hit TTGCCGCGCGTGGTCTCATTGTCACCCTCCCCGGGCTCGCACACCCCAAC 5 0.125 No Hit CCAAAATCTTTTCCACTCCAAACTTCTCTGCCGAAACATGACGTCGCATT 5 0.125 No Hit GATGCCAGCAGTGTAGCTGCTTACCTTCTTGACACACTGGGTCTTGTCAG 5 0.125 No Hit ATCGTCTTCAAAATTGGTTACCCTGTACAAAGACTCAGCCAAGTCCCTAT 5 0.125 No Hit TGCAGCGCCAGCATCACCATGCCCTCGTTCCACTTGATGTATGTACTAGT 5 0.125 No Hit GGGGGTGTTGTTGGCGAAGCCTGCCAATGTATTCGTGATCTTGGTATGCC 5 0.125 No Hit CCAACTCTAGCATCTACAAGTTTAAAGAATACGGTATGGCTTCACCTCCT 5 0.125 No Hit GATCGATTGAGCTTGTTTCTATATACTTCGCATGGTAATTATCCATTCCT 5 0.125 No Hit ACGAAAATGAGAGCTTGGCTCACAATACTGACTTGAAGGTACAAAGCCCC 5 0.125 No Hit CAGAACAATGAAGAGAATTGAAGACCATCAATCCATCTCTCAGAAGAAGA 5 0.125 No Hit CCGATCTTCTCGTGTCCTTGGCTCCTCAGACCGAGAGGATCCTCCTGATT 5 0.125 No Hit AAAGCTGAAAGATAACCTAGAAAAAACATTCCTGTTTAATGTAAAACTAA 5 0.125 No Hit GCAGCGGAGATCTAAAATGAAGGGGAAAAGGGTTATGGATCGCTAATGCA 5 0.125 No Hit AGATCTTTCTTACATATGGACCATAACAATCACTCTGCTTGTAGAGAATT 5 0.125 No Hit CACCCGATTCACAAAGCTTTGAAAATCCATATTGATCTTATTCTTCCTAT 5 0.125 No Hit CGGGGGGGTTGAAGTGTGATTTGCTGGAGCATCTCTGACCATATTATTGT 5 0.125 No Hit TGCCTCTTGACTATCTGCGCTAGCAACCAGATATAGGGGATAAACAGGTT 5 0.125 No Hit GTGAACTTGACCTCAATTGGGCTAACGTTCTTTCCGATTGACCTGAAATT 5 0.125 No Hit TTTGTATTTGTTTAGCTACCATTTTCACTCTCCTTGGATTTGCCATGCAT 5 0.125 No Hit CTGTAACAAACTTCTCTAAGCGTGTGGATTGGTAGAGATCATAGTAAATA 5 0.125 No Hit GATCTTCACCAGCACCCTGTCACCCAAAGGCGTAATGGAAGTGTACTTGG 5 0.125 No Hit GTTCCTTAGAACTATCGCTATGATAAGATGGTTGAAGCTGCCTCAACATA 5 0.125 No Hit CTGAGTTTCACTTCTGTCCAAAATGGAAGGATGGTCGCATAATGCAAGAA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.037500000000000006 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.0875 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.21250000000000002 0.0 0.0 0.0 0.0 84-85 0.2375 0.0 0.0 0.0 0.0 86-87 0.275 0.0 0.0 0.0 0.0 88-89 0.35 0.0 0.0 0.0 0.0 90-91 0.475 0.0 0.0 0.0 0.0 92-93 0.5625 0.0 0.0 0.0 0.0 94-95 0.7125 0.0 0.0 0.0 0.0 96-97 0.825 0.0 0.0 0.0 0.0 98-99 1.0 0.0 0.0 0.0 0.0 100-101 1.2375 0.0 0.0 0.0 0.0 102-103 1.3125 0.0 0.0 0.0 0.0 104-105 1.6375000000000002 0.0 0.0 0.0 0.0 106-107 1.975 0.0 0.0 0.0 0.0 108-109 2.3499999999999996 0.0 0.0 0.0 0.0 110-111 2.5999999999999996 0.0 0.0 0.0 0.0 112-113 3.0875 0.0 0.0 0.0 0.0 114-115 3.3875 0.0 0.0 0.0 0.0 116-117 3.8625 0.0 0.0 0.0 0.0 118-119 4.2375 0.0 0.0 0.0 0.0 120-121 4.675 0.0 0.0 0.0 0.0 122-123 5.0875 0.0 0.0 0.0 0.0 124-125 5.4125 0.0 0.0 0.0 0.0 126-127 5.8375 0.0 0.0 0.0 0.0 128-129 6.3875 0.0 0.0 0.0 0.0 130-131 6.9375 0.0 0.0 0.0 0.0 132-133 7.6625 0.0 0.0 0.0 0.0 134-135 8.162500000000001 0.0 0.0 0.0 0.0 136-137 9.3125 0.0 0.0 0.0 0.0 138-139 9.8375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGTTCA 10 0.006830828 145.0 8 CAAAGTA 10 0.006830828 145.0 3 GCCAAAG 10 0.006830828 145.0 1 AAAGTAG 10 0.006830828 145.0 4 GCATCAT 10 0.006830828 145.0 5 AGTTCAA 10 0.006830828 145.0 9 GTAGTTC 10 0.006830828 145.0 7 >>END_MODULE SRR13695437 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695437_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.2895 37.0 37.0 37.0 37.0 37.0 2 36.1385 37.0 37.0 37.0 37.0 37.0 3 36.2165 37.0 37.0 37.0 37.0 37.0 4 36.247 37.0 37.0 37.0 37.0 37.0 5 36.27 37.0 37.0 37.0 37.0 37.0 6 36.343 37.0 37.0 37.0 37.0 37.0 7 36.28 37.0 37.0 37.0 37.0 37.0 8 36.2935 37.0 37.0 37.0 37.0 37.0 9 36.2915 37.0 37.0 37.0 37.0 37.0 10-14 36.3253 37.0 37.0 37.0 37.0 37.0 15-19 36.2907 37.0 37.0 37.0 37.0 37.0 20-24 36.273450000000004 37.0 37.0 37.0 37.0 37.0 25-29 36.17785 37.0 37.0 37.0 37.0 37.0 30-34 36.13965 37.0 37.0 37.0 37.0 37.0 35-39 36.13615 37.0 37.0 37.0 37.0 37.0 40-44 36.14705 37.0 37.0 37.0 37.0 37.0 45-49 36.1274 37.0 37.0 37.0 37.0 37.0 50-54 36.025549999999996 37.0 37.0 37.0 37.0 37.0 55-59 36.05745 37.0 37.0 37.0 37.0 37.0 60-64 36.09535 37.0 37.0 37.0 37.0 37.0 65-69 35.9596 37.0 37.0 37.0 37.0 37.0 70-74 35.99115 37.0 37.0 37.0 37.0 37.0 75-79 35.877700000000004 37.0 37.0 37.0 37.0 37.0 80-84 35.9341 37.0 37.0 37.0 37.0 37.0 85-89 35.81785 37.0 37.0 37.0 37.0 37.0 90-94 35.755399999999995 37.0 37.0 37.0 37.0 37.0 95-99 35.75295 37.0 37.0 37.0 37.0 37.0 100-104 35.751549999999995 37.0 37.0 37.0 37.0 37.0 105-109 35.77445 37.0 37.0 37.0 37.0 37.0 110-114 35.677749999999996 37.0 37.0 37.0 37.0 37.0 115-119 35.66485 37.0 37.0 37.0 37.0 37.0 120-124 35.59505 37.0 37.0 37.0 37.0 37.0 125-129 35.6503 37.0 37.0 37.0 37.0 37.0 130-134 35.47625 37.0 37.0 37.0 37.0 37.0 135-139 35.42865 37.0 37.0 37.0 34.6 37.0 140-144 35.28895 37.0 37.0 37.0 32.2 37.0 145-149 35.29135 37.0 37.0 37.0 32.2 37.0 150-151 34.942750000000004 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.0 15 1.0 16 0.0 17 0.0 18 2.0 19 3.0 20 4.0 21 3.0 22 2.0 23 6.0 24 9.0 25 2.0 26 12.0 27 10.0 28 15.0 29 17.0 30 23.0 31 31.0 32 57.0 33 109.0 34 192.0 35 621.0 36 2666.0 37 212.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.52130325814536 19.899749373433583 12.355889724310778 29.223057644110273 2 27.3 25.224999999999998 32.0 15.475 3 18.4 28.875 31.424999999999997 21.3 4 24.55 34.125 25.025 16.3 5 25.95 35.5 22.575 15.975 6 20.849999999999998 39.45 23.1 16.6 7 18.925 23.25 38.35 19.475 8 20.95 25.374999999999996 29.2 24.474999999999998 9 21.8 24.925 30.825000000000003 22.45 10-14 23.1 30.17 26.055 20.674999999999997 15-19 22.17 29.189999999999998 27.800000000000004 20.84 20-24 22.557406573615488 29.571264195307418 27.685226874781126 20.18610235629596 25-29 22.566925193895422 29.592194145609206 27.215411558669 20.62546910182637 30-34 22.919897933656877 28.443488267373795 28.033221594036124 20.603392204933208 35-39 22.142178198008907 28.79583771074091 28.130471759467707 20.931512331782482 40-44 22.469605243408218 28.893780957622457 27.878120778506027 20.758493020463302 45-49 21.85811486892135 29.442665599359614 28.056834100460275 20.642385431258756 50-54 22.047125919255592 28.945920256140877 28.735804692580917 20.27114913202261 55-59 22.93219914936202 28.931698774080562 27.575681761320993 20.560420315236428 60-64 22.202211216168894 28.170493771574368 28.175496523087702 21.451798489169043 65-69 22.814125650260102 28.661464585834334 26.735694277711087 21.788715486194477 70-74 23.937953465098825 27.530647985989493 27.875906930197647 20.655491618714038 75-79 22.490743520464328 28.394876413489445 27.64935454818373 21.465025517862504 80-84 22.391195597798898 28.189094547273637 27.92896448224112 21.490745372686344 85-89 22.566925193895422 28.641481110833123 27.9009256942707 20.89066800100075 90-94 23.193916349809886 28.50210126075645 27.661596958174904 20.642385431258756 95-99 22.332282755515536 27.865325929261093 28.145480014007706 21.65691130121567 100-104 23.232424318238678 27.91593695271454 27.850888166124594 21.000750562922192 105-109 23.177383037277956 28.791593695271455 27.110332749562172 20.920690517888417 110-114 24.33338336084847 27.425083796087847 27.41007554154785 20.831457301515837 115-119 23.91293470102577 29.086815111333504 27.235426569927444 19.764823617713283 120-124 23.837878408806603 28.946710032524393 26.885163872904677 20.330247685764324 125-129 24.10687481236866 29.175422795957168 26.153307315120582 20.564395076553588 130-134 25.063797848386287 28.586439829872408 27.2604453340005 19.089316987740805 135-139 24.95371528646485 27.795846885163872 27.705779334500875 19.544658493870404 140-144 25.343939166541595 27.870328680774424 27.18995447496123 19.595777677722747 145-149 25.924443332499376 28.251188391293468 26.579934951213406 19.244433324993746 150-151 26.99524643482612 26.65749311983988 26.8951713785339 19.4520890668001 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 1.0 8 1.0 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.0 16 0.0 17 0.0 18 1.0 19 3.0 20 2.5 21 1.0 22 1.5 23 2.0 24 1.5 25 3.5 26 8.0 27 12.0 28 12.5 29 16.5 30 23.5 31 27.5 32 33.5 33 40.5 34 54.5 35 72.5 36 96.5 37 135.5 38 162.0 39 174.5 40 213.5 41 223.5 42 222.5 43 266.5 44 289.0 45 262.5 46 246.5 47 261.0 48 248.5 49 185.5 50 146.0 51 117.5 52 88.0 53 78.5 54 66.5 55 55.5 56 41.5 57 28.5 58 16.0 59 14.5 60 12.5 61 7.5 62 4.0 63 5.0 64 4.5 65 3.0 66 1.5 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.055 25-29 0.075 30-34 0.065 35-39 0.055 40-44 0.065 45-49 0.06 50-54 0.055 55-59 0.075 60-64 0.055 65-69 0.04 70-74 0.075 75-79 0.06999999999999999 80-84 0.05 85-89 0.075 90-94 0.06 95-99 0.055 100-104 0.075 105-109 0.075 110-114 0.055 115-119 0.075 120-124 0.075 125-129 0.06999999999999999 130-134 0.075 135-139 0.075 140-144 0.055 145-149 0.075 150-151 0.075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.5 #Duplication Level Percentage of deduplicated Percentage of total 1 69.51851851851852 46.925 2 19.85185185185185 26.8 3 6.333333333333334 12.825000000000001 4 2.925925925925926 7.9 5 0.8148148148148148 2.75 6 0.33333333333333337 1.35 7 0.037037037037037035 0.17500000000000002 8 0.1111111111111111 0.6 9 0.0 0.0 >10 0.07407407407407407 0.675 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAAT 14 0.35000000000000003 No Hit AGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATC 13 0.325 No Hit TGACTCTTGGTTTTAACGCTTGTTAAATGGCTGAGGATCACGTTCCATGT 8 0.2 No Hit ATTTTGTCCACAAAAAGAACAAATGGCTGCAACTCTCTCGGTCCGGTCCA 8 0.2 No Hit AGAAGGCAACCAAAAAAACCCTAGGGGCTGAACATTTTTCCATCTCCTGC 8 0.2 No Hit ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA 7 0.17500000000000002 No Hit TGAGAGGTTTGGCTGGGACAATGAAGATAAAGTTGGGTGGAGTAGAGTCA 6 0.15 No Hit TGAGATTCACTCCGTGTACAACTACGAAACTACTGAACTTGTTCATGAGA 6 0.15 No Hit CCCCCTTGGATTTGGGAAAGACGAGAAGTCGTTGAAAGACTTGAAGTTGA 6 0.15 No Hit GAAAGAACAGGAAGTGAGGGCATACTGGGAAATTGAAAGGCAAGAGATGC 6 0.15 No Hit GAATGTCTGCGGTTCTGTTGTGCAGACAATAGGACTCGCTGACGGTAACA 6 0.15 No Hit GGACCAGTGGACTCTAGCAGAAAACAGAAATGCATTCTCAAGAATTCTGT 6 0.15 No Hit GGGGAGATGAAGTTAAGGTTTGCAATATTCTCGGAGAGTTTGGATTTGAT 6 0.15 No Hit GGAAGGTCTCATCTATAGGGATATTCGGCTAATAAATTGGGACTGCACCT 6 0.15 No Hit ATCTCTGCCTCCAACACTGGTGGTGCATGGGATAATGCCAAGAAATATAT 6 0.15 No Hit CCAGGCTCCCACCCCTTTGATGAGATACTTTACTGCAATATTGGAAATCC 5 0.125 No Hit GTCATTCTTCTTCTCCATTGCCATGACCAATGCCTTCTTCACCACCTCAT 5 0.125 No Hit AGAAATCTCATCAAACATGGTCACTCTGGAGATCTCTACCAGGGTTTTTT 5 0.125 No Hit CACTAAAGGACTGGGATCATCTGAAGGAGCTTCTTTCTGTTTGTGAAGCT 5 0.125 No Hit CTTCTCTTATCATATCTCATAATAAAACCCACTCTCTCTACATCCACACT 5 0.125 No Hit CCACAATCCTCGACACAATGATCAATGTACACCGTCACCTTCTACACCAA 5 0.125 No Hit GGAGTATGTTCCAGAAGCTCCAGAAAGTCTGTCTGAGTTTGAATCTTCTC 5 0.125 No Hit GTGTTTTCAATAGAGTTCACCATAATCCTCTAGAGCATGTTTCCTTTGGG 5 0.125 No Hit GGTTGCATCACCGGGGAAGAAGGCTGTAGCGGTGAAGCTCAAGAGTGTGA 5 0.125 No Hit AATTACTCCCTGCTGCATCAAATGCAGGGAATGAAAAATGCGGAGGTTGA 5 0.125 No Hit GGCTTCCTCACCTGTTTGTTGGAAACTTGCTCAAAGGACAAACTGTTGGT 5 0.125 No Hit ATTATTTCAAAAACTCCCTATTTTTCTTGGTTTCTCCTTCGAAATATCCT 5 0.125 No Hit TGGGCTTTCAATTGCTCAAAGCAACCGTGTTGCAGGAAAGAATCCCTTAA 5 0.125 No Hit GGAGAAAACTGTAATCTCTCCTCCTCCTCTCGTTGAAGAGAAGGTTGAGG 5 0.125 No Hit CAGAAACTAACAAACTAAGATAATTTTTTTTGATCGAGAAATTCTGGGGG 5 0.125 No Hit AATGGAGAGGTGCAGTACTTGCATCCTAAGGATGGTGTCTACCCTGAGAA 5 0.125 No Hit GTCTGAAAACTTTTATGGCATATCACATGTGTCTGTTGCTCTTAATAATT 5 0.125 No Hit CATGAAAGGTGCCTATAAGCAAGCTGATGGGCGGAAGCTTGATGGAAGAA 5 0.125 No Hit GAAAGTTGCCTGGTGCTCTTGAAGCTCATGTTAATGGATTCCGATTTTCT 5 0.125 No Hit CCTAAATGACGGTACAATTATGACAATCTCAAAGGATAGAGTGAAGCCAT 5 0.125 No Hit AGAATGCTCATTTTGAAGATACAAAGCTGAAAAAAACCTTTACATTCTTT 5 0.125 No Hit GGTATATATTTTTTACCATAGTTGGTTAGAGAAAAGTGTGGTTCTTATCT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.037500000000000006 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.0875 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.21250000000000002 0.0 0.0 0.0 0.0 84-85 0.2375 0.0 0.0 0.0 0.0 86-87 0.275 0.0 0.0 0.0 0.0 88-89 0.35 0.0 0.0 0.0 0.0 90-91 0.475 0.0 0.0 0.0 0.0 92-93 0.5625 0.0 0.0 0.0 0.0 94-95 0.7125 0.0 0.0 0.0 0.0 96-97 0.85 0.0 0.0 0.0 0.0 98-99 0.9875 0.0 0.0 0.0 0.0 100-101 1.1875 0.0 0.0 0.0 0.0 102-103 1.2625 0.0 0.0 0.0 0.0 104-105 1.5875 0.0 0.0 0.0 0.0 106-107 1.9249999999999998 0.0 0.0 0.0 0.0 108-109 2.2750000000000004 0.0 0.0 0.0 0.0 110-111 2.5 0.0 0.0 0.0 0.0 112-113 2.9875 0.0 0.0 0.0 0.0 114-115 3.2875 0.0 0.0 0.0 0.0 116-117 3.7874999999999996 0.0 0.0 0.0 0.0 118-119 4.1375 0.0 0.0 0.0 0.0 120-121 4.55 0.0 0.0 0.0 0.0 122-123 5.0125 0.0 0.0 0.0 0.0 124-125 5.3375 0.0 0.0 0.0 0.0 126-127 5.7625 0.0 0.0 0.0 0.0 128-129 6.325 0.0 0.0 0.0 0.0 130-131 6.875 0.0 0.0 0.0 0.0 132-133 7.5875 0.0 0.0 0.0 0.0 134-135 8.0875 0.0 0.0 0.0 0.0 136-137 9.2375 0.0 0.0 0.0 0.0 138-139 9.775 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTGAGTG 10 0.006830828 145.0 3 CATTGAA 10 0.006830828 145.0 8 TGGTACA 10 0.006830828 145.0 8 TAAAACT 10 0.006830828 145.0 3 CCTGAGT 10 0.006830828 145.0 2 TATTCAT 10 0.006830828 145.0 4 GTGGTAC 10 0.006830828 145.0 7 TCCTGAG 10 0.006830828 145.0 1 ATTCATT 10 0.006830828 145.0 5 ACTATTC 10 0.006830828 145.0 2 CTATTCA 10 0.006830828 145.0 3 TTCATTG 20 3.5877043E-4 108.75 6 TCATTGA 20 3.5877043E-4 108.75 7 >>END_MODULE Read 789826 spots for SRR13695437.sra Written 789826 spots for SRR13695437.sra Read 789826 spots for SRR13695437.sra Written 789826 spots for SRR13695437.sra Read 789826 spots for SRR13695437.sra Written 789826 spots for SRR13695437.sra Read 789826 spots for SRR13695437.sra Written 789826 spots for SRR13695437.sra Read 789826 spots for SRR13695437.sra Written 789826 spots for SRR13695437.sra Read 789826 spots for SRR13695437.sra Written 789826 spots for SRR13695437.sra Read 789826 spots for SRR13695437.sra Written 789826 spots for SRR13695437.sra Read 789826 spots for SRR13695437.sra Written 789826 spots for SRR13695437.sra Read 789826 spots for SRR13695437.sra Written 789826 spots for SRR13695437.sra Read 789838 spots for SRR13695437.sra Written 789838 spots for SRR13695437.sra Read 789826 spots for SRR13695437.sra Written 789826 spots for SRR13695437.sra Read 789826 spots for SRR13695437.sra Written 789826 spots for SRR13695437.sra Read 789826 spots for SRR13695437.sra Written 789826 spots for SRR13695437.sra Read 789826 spots for SRR13695437.sra Written 789826 spots for SRR13695437.sra Read 789826 spots for SRR13695437.sra Written 789826 spots for SRR13695437.sra Read 789826 spots for SRR13695437.sra Written 789826 spots for SRR13695437.sra Read 789826 spots for SRR13695437.sra Written 789826 spots for SRR13695437.sra Read 789826 spots for SRR13695437.sra Written 789826 spots for SRR13695437.sra Read 789826 spots for SRR13695437.sra Written 789826 spots for SRR13695437.sra Read 789826 spots for SRR13695437.sra Written 789826 spots for SRR13695437.sra SRR ids: ['SRR13695437.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_7xifueu1 SRR13695437.sra spots: 15796532 blocks: [[1, 789826], [789827, 1579652], [1579653, 2369478], [2369479, 3159304], [3159305, 3949130], [3949131, 4738956], [4738957, 5528782], [5528783, 6318608], [6318609, 7108434], [7108435, 7898260], [7898261, 8688086], [8688087, 9477912], [9477913, 10267738], [10267739, 11057564], [11057565, 11847390], [11847391, 12637216], [12637217, 13427042], [13427043, 14216868], [14216869, 15006694], [15006695, 15796532]] SRR13695437 file size 5346652 SRR13695437 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695437 SRR13695437_1.fastq SRR13695437_2.fastq Input file: SRR13695437_1.fastq Paired file: SRR13695437_2.fastq trimmed: SRR13695437-trimmed-pair1.fastq, SRR13695437-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 03:01:13 2025 >> started Wed Feb 12 03:01:29 2025 >> done (16.276s) 15796532 read pairs processed; of these: 104 ( 0.00%) short read pairs filtered out after trimming by size control 1170 ( 0.01%) empty read pairs filtered out after trimming by size control 15795258 (99.99%) read pairs available; of these: 2140494 (13.55%) trimmed read pairs available after processing 13654764 (86.45%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 4 0.00% 20 1 0.00% 21 4 0.00% 22 5 0.00% 23 0 0.00% 24 2 0.00% 25 2 0.00% 26 1 0.00% 27 7 0.00% 28 3 0.00% 29 6 0.00% 30 3 0.00% 31 4 0.00% 32 2 0.00% 33 5 0.00% 34 2 0.00% 35 7 0.00% 36 9 0.00% 37 10 0.00% 38 7 0.00% 39 11 0.00% 40 20 0.00% 41 6 0.00% 42 13 0.00% 43 15 0.00% 44 12 0.00% 45 23 0.00% 46 12 0.00% 47 19 0.00% 48 23 0.00% 49 33 0.00% 50 34 0.00% 51 62 0.00% 52 57 0.00% 53 55 0.00% 54 64 0.00% 55 79 0.00% 56 84 0.00% 57 109 0.00% 58 125 0.00% 59 138 0.00% 60 170 0.00% 61 191 0.00% 62 248 0.00% 63 294 0.00% 64 309 0.00% 65 375 0.00% 66 397 0.00% 67 417 0.00% 68 559 0.00% 69 638 0.00% 70 743 0.00% 71 801 0.01% 72 993 0.01% 73 1099 0.01% 74 1211 0.01% 75 1497 0.01% 76 1586 0.01% 77 1878 0.01% 78 2069 0.01% 79 2451 0.02% 80 2531 0.02% 81 3118 0.02% 82 3584 0.02% 83 4030 0.03% 84 4470 0.03% 85 4909 0.03% 86 5179 0.03% 87 6136 0.04% 88 6652 0.04% 89 6886 0.04% 90 7976 0.05% 91 8410 0.05% 92 8923 0.06% 93 10069 0.06% 94 10529 0.07% 95 11508 0.07% 96 12398 0.08% 97 13164 0.08% 98 13891 0.09% 99 14903 0.09% 100 15950 0.10% 101 16287 0.10% 102 17752 0.11% 103 18354 0.12% 104 19181 0.12% 105 20547 0.13% 106 21243 0.13% 107 22387 0.14% 108 23627 0.15% 109 24410 0.15% 110 24672 0.16% 111 25859 0.16% 112 26918 0.17% 113 27554 0.17% 114 28906 0.18% 115 30495 0.19% 116 30929 0.20% 117 32267 0.20% 118 32906 0.21% 119 33339 0.21% 120 35165 0.22% 121 35424 0.22% 122 36323 0.23% 123 37275 0.24% 124 38828 0.25% 125 38988 0.25% 126 40176 0.25% 127 41406 0.26% 128 42168 0.27% 129 42658 0.27% 130 44750 0.28% 131 44914 0.28% 132 44951 0.28% 133 46167 0.29% 134 46483 0.29% 135 47471 0.30% 136 48070 0.30% 137 48818 0.31% 138 50178 0.32% 139 51533 0.33% 140 51662 0.33% 141 52298 0.33% 142 53901 0.34% 143 53225 0.34% 144 55248 0.35% 145 55351 0.35% 146 55138 0.35% 147 56349 0.36% 148 57609 0.36% 149 57502 0.36% 150 58612 0.37% 151 13654764 86.45% 15795258 reads passed initial QC criterion=sequence-density sequence-density=0.29 sequence-density-rank=1 fanout-score=2.60 fanout-score-rank=22 prefix-density=0.32 prefix-fanout=2.4 sequence=TTAGCCTTTCTGGT criterion=fanout-score sequence-density=0.01 sequence-density-rank=43 fanout-score=404.54 fanout-score-rank=1 prefix-density=0.28 prefix-fanout=18.7 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGAGTATGAATGTGTTCTCTGTG criterion=sequence-density sequence-density=0.42 sequence-density-rank=1 fanout-score=2.21 fanout-score-rank=29 prefix-density=0.43 prefix-fanout=2.1 sequence=TGTAAGAGATGGCTTCCTC criterion=fanout-score sequence-density=0.16 sequence-density-rank=22 fanout-score=27.34 fanout-score-rank=1 prefix-density=0.40 prefix-fanout=10.8 sequence=AAAGAAAAGAAAA SRR13695437 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 03:02:09 Started mapping on | Feb 12 03:02:09 Finished on | Feb 12 03:04:09 Mapping speed, Million of reads per hour | 473.86 Number of input reads | 15795258 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 14794947 Uniquely mapped reads % | 93.67% Average mapped length | 294.06 Number of splices: Total | 14354368 Number of splices: Annotated (sjdb) | 14020748 Number of splices: GT/AG | 14063123 Number of splices: GC/AG | 221548 Number of splices: AT/AC | 8427 Number of splices: Non-canonical | 61270 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 2.87 Insertion rate per base | 0.02% Insertion average length | 2.42 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 358943 % of reads mapped to multiple loci | 2.27% Number of reads mapped to too many loci | 107699 % of reads mapped to too many loci | 0.68% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.23% % of reads unmapped: other | 0.15% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 641538 641538 641538 N_multimapping 358943 358943 358943 N_noFeature 707986 14383010 967476 N_ambiguous 238154 1844 84302 UnstrandedReadsAssigned:13848807 PositiveStrandReadsAssigned:410093 NegativeStrandReadsAssigned:13743169 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695437 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695437-trimmed-pair1.fastq SRR13695437-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 15,795,258 reads, 13,736,554 reads pseudoaligned [quant] estimated average fragment length: 243.737 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,007 rounds 52401 SRR13695437.ke.tsv 34699 SRR13695437.se.tsv 87100 total ==> SRR13695437.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1775.26 563 19.8787 Potri.005G024800.1.v4.1 1035 792.263 424 33.5459 Potri.004G059700.1.v4.1 961 718.362 0 0 Potri.007G009000.2.v4.1 1416 1173.26 0 0 Potri.003G141000.2.v4.1 2943 2700.26 958.53 22.2506 Potri.016G087400.1.v4.1 270 87.3887 764 548 Potri.015G069301.1.v4.1 564 329.671 0 0 Potri.010G195200.1.v4.1 1773 1530.26 64 2.62154 Potri.012G127500.1.v4.1 977 734.326 177 15.1087 ==> SRR13695437.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 50 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 241 Potri.001G212900.v4.1 1 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 13 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 18 SRR13695437 completed mapping pipeline successfully