Starting /dee2/code/volunteer_pipeline.sh SRR13695438 current disk space = 3049189040128 free memory = 1123158152 SRR13695438 SRAfilesize adad056834981dfbd9c68108d7e112e2 SRR13695438.sra SRR13695438.sra file validated SRR13695438 is paired end SRR13695438 is conventional basespace SRR13695438 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695438_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.603 37.0 37.0 37.0 37.0 37.0 2 36.3065 37.0 37.0 37.0 37.0 37.0 3 36.5795 37.0 37.0 37.0 37.0 37.0 4 36.574 37.0 37.0 37.0 37.0 37.0 5 36.6385 37.0 37.0 37.0 37.0 37.0 6 36.582 37.0 37.0 37.0 37.0 37.0 7 36.552 37.0 37.0 37.0 37.0 37.0 8 36.5925 37.0 37.0 37.0 37.0 37.0 9 36.585 37.0 37.0 37.0 37.0 37.0 10-14 36.5937 37.0 37.0 37.0 37.0 37.0 15-19 36.5413 37.0 37.0 37.0 37.0 37.0 20-24 36.5169 37.0 37.0 37.0 37.0 37.0 25-29 36.46849999999999 37.0 37.0 37.0 37.0 37.0 30-34 36.4362 37.0 37.0 37.0 37.0 37.0 35-39 36.4395 37.0 37.0 37.0 37.0 37.0 40-44 36.4476 37.0 37.0 37.0 37.0 37.0 45-49 36.412099999999995 37.0 37.0 37.0 37.0 37.0 50-54 36.4135 37.0 37.0 37.0 37.0 37.0 55-59 36.384100000000004 37.0 37.0 37.0 37.0 37.0 60-64 36.4059 37.0 37.0 37.0 37.0 37.0 65-69 36.3411 37.0 37.0 37.0 37.0 37.0 70-74 36.331999999999994 37.0 37.0 37.0 37.0 37.0 75-79 36.3164 37.0 37.0 37.0 37.0 37.0 80-84 36.273799999999994 37.0 37.0 37.0 37.0 37.0 85-89 36.243500000000004 37.0 37.0 37.0 37.0 37.0 90-94 36.17629999999999 37.0 37.0 37.0 37.0 37.0 95-99 36.139300000000006 37.0 37.0 37.0 37.0 37.0 100-104 36.1329 37.0 37.0 37.0 37.0 37.0 105-109 36.11710000000001 37.0 37.0 37.0 37.0 37.0 110-114 36.1367 37.0 37.0 37.0 37.0 37.0 115-119 36.1325 37.0 37.0 37.0 37.0 37.0 120-124 36.02550000000001 37.0 37.0 37.0 37.0 37.0 125-129 35.9805 37.0 37.0 37.0 37.0 37.0 130-134 35.983999999999995 37.0 37.0 37.0 37.0 37.0 135-139 35.9664 37.0 37.0 37.0 37.0 37.0 140-144 35.8195 37.0 37.0 37.0 37.0 37.0 145-149 35.7446 37.0 37.0 37.0 37.0 37.0 150-151 35.535 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 1.0 24 1.0 25 2.0 26 5.0 27 11.0 28 10.0 29 22.0 30 25.0 31 46.0 32 42.0 33 63.0 34 116.0 35 302.0 36 2995.0 37 359.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.074999999999996 12.375 9.425 40.125 2 20.483140412682435 12.40563663814796 37.06592853548062 30.04529441368898 3 18.8 17.849999999999998 26.700000000000003 36.65 4 23.575 25.15 23.974999999999998 27.3 5 23.799999999999997 31.974999999999998 24.925 19.3 6 21.9 33.75 22.725 21.625 7 13.25 27.925 40.849999999999994 17.974999999999998 8 16.400000000000002 27.275 32.025 24.3 9 17.9 25.374999999999996 34.25 22.475 10-14 19.439999999999998 30.330000000000002 27.375 22.855 15-19 19.505 29.315 27.975 23.205000000000002 20-24 19.855 28.494999999999997 28.7 22.95 25-29 20.22 28.610000000000003 27.889999999999997 23.28 30-34 19.33 28.865000000000002 28.475 23.330000000000002 35-39 20.150000000000002 28.59 28.37 22.89 40-44 19.265 29.654999999999998 27.865000000000002 23.215 45-49 20.59 27.925 27.139999999999997 24.345 50-54 20.064999999999998 29.304999999999996 27.855 22.775000000000002 55-59 19.689999999999998 28.935 28.044999999999998 23.330000000000002 60-64 19.975 28.115000000000002 27.495000000000005 24.415 65-69 18.965 29.115000000000002 27.91 24.01 70-74 19.689999999999998 29.849999999999998 27.375 23.085 75-79 19.915 28.38 27.700000000000003 24.005000000000003 80-84 19.93 28.74 28.28 23.05 85-89 19.900000000000002 28.384999999999998 27.889999999999997 23.825 90-94 19.755 28.744999999999997 27.67 23.830000000000002 95-99 20.235 28.365000000000002 28.205000000000002 23.195 100-104 19.72 28.425 27.744999999999997 24.11 105-109 20.200000000000003 29.175 27.655 22.97 110-114 20.25 28.43 27.185 24.135 115-119 20.535 29.654999999999998 27.005000000000003 22.805 120-124 20.31 28.194999999999997 27.395000000000003 24.099999999999998 125-129 21.17 28.134999999999998 27.345000000000002 23.35 130-134 20.724999999999998 28.439999999999998 26.845000000000002 23.990000000000002 135-139 21.105 28.475 27.034999999999997 23.385 140-144 20.455000000000002 28.46 26.955000000000002 24.13 145-149 20.849999999999998 28.549999999999997 26.455000000000002 24.145 150-151 20.4625 29.5875 26.55 23.400000000000002 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 0.5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 1.0 24 3.0 25 7.0 26 9.0 27 11.0 28 23.0 29 22.0 30 13.0 31 24.0 32 36.5 33 44.0 34 59.0 35 92.5 36 115.0 37 126.0 38 145.5 39 163.5 40 186.0 41 220.5 42 241.0 43 245.0 44 249.0 45 255.0 46 254.5 47 245.0 48 230.0 49 197.0 50 172.5 51 138.5 52 103.5 53 81.0 54 68.5 55 60.0 56 43.0 57 34.5 58 26.0 59 18.5 60 11.0 61 7.0 62 3.5 63 2.0 64 2.5 65 2.5 66 2.0 67 0.5 68 0.5 69 0.0 70 1.0 71 1.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.65 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.05 #Duplication Level Percentage of deduplicated Percentage of total 1 70.53637031594417 48.0 2 18.772961058045553 25.55 3 6.649522409992653 13.575000000000001 4 2.5716385011021305 7.000000000000001 5 0.5878030859662012 2.0 6 0.5510653930933138 2.25 7 0.2571638501102131 1.225 8 0.07347538574577515 0.4 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACAGGGAGGTACTTCACACAGACAAAAACAACAACAGAATGTATGGCAGG 8 0.2 No Hit GTGCAAGCTCTCGGGATGGTGTGAGAAGAAGTCAAGTATCCTCCAGTTTT 8 0.2 No Hit GTCGGCCATTGTTGTATCCTGTGATTTGGATGATGGTTCTTCTCCCTTTT 7 0.17500000000000002 No Hit ATCGAGATACACAACCATATCATAGATTTTACAACTAAATACAAAAGCAA 7 0.17500000000000002 No Hit CTCGGATTTTTGAATCAGGGGTAGTGACTTAACAGCTCGCTCAATCTTGG 7 0.17500000000000002 No Hit GGCTTGTTCAAATTCCCTCCGATTGTTACTGTGTCAAGTGGCAGCATCAC 7 0.17500000000000002 No Hit GTCATCCTTAGAATTGCATCTCTAACATTTTCAAGGACATCAAAATCAGT 7 0.17500000000000002 No Hit TGTGCCTTCAGGGTCTGAAAGTCCGAGTGGGTCGAATCCGAAGTCACCTG 7 0.17500000000000002 No Hit GTGGCTGGTTTGCTCCTCCAAGAAGATGCAGGTGACCGATAATTGGCCAT 7 0.17500000000000002 No Hit TGCGACATGGTTGGCAAGAATCCTTCTGCGAATTTAGCAACAACCGAAGA 6 0.15 No Hit CTGCACTTTGTTTCTTGATAGGTGACTTTCCATTGGCGTTTTCAGCAAGT 6 0.15 No Hit GTTGGCTCTAAGCAGATCTTCTTAGCATTGTATTTGCCGGGCTTGAAGGC 6 0.15 No Hit CCCTCACTAGTCCTCTGACTTAGTGTCCCCTTCTGGTCGCGTCTGAAATG 6 0.15 No Hit ACTCGGTATCCATTTCCAAGAACATTTTCCGAACGTTAACAGTCAGCCCA 6 0.15 No Hit TCTTTGTTCTGGAACATGGTACCTGCTCAGAGTGCCATCTCAGCTTGCAA 6 0.15 No Hit GGGGAAAATAGGCAGTGTTGATTTCTTTCTCAAGTATCTATCCTCTACAA 6 0.15 No Hit GTGCATTGGCATATTCTGCTAAAACCTTAGTCTTGGCAGGGTAACAAACT 6 0.15 No Hit GTTGTCAAGGTGGTCCAAGAGGTTCTCCAGGGGACCTTTTCCAGTAACAA 6 0.15 No Hit GTTGAGAGAGAGAGAGGGAAGCAGAGAGAGGAACAGAAACAGTGGAAGAA 6 0.15 No Hit GATAAATTCTCACAGTACATGTGCTCCTACTACAATACAATTTAACTTGG 6 0.15 No Hit TATTTATCAAGGTAACCAATCGACTGTTTAATATCCTAAATAGTAGAATA 6 0.15 No Hit CACAAAACTCAATGTTTGACTAACATGGCCTCCGTATTCCTCTTCCTCGC 6 0.15 No Hit GGAAAGAACAAAAACCATATCAGCTAATTCAAGTCTCAGGAATGCTTCCA 6 0.15 No Hit CTCGTTTACAGATGAGCAGCAAACAAATTGTGCCCAGTGTGGTCTTCCCT 6 0.15 No Hit GGGATGTTCTCTCTCCTAAATTTCTAATCTGACATGTATAATATGCACAA 5 0.125 No Hit GGCCTTCGATCCAACGTGGTTTGTATGCGGGGCCTTATTCGAACATTGCC 5 0.125 No Hit CTTTTTTTACCATTCTAGTCCCCATCACATCTCTGTTTCTTTTCCCTCGT 5 0.125 No Hit CTCCTTTCCTTTGTAGACCCTGGTAACCTGCGATATCCATGAATTAAGCT 5 0.125 No Hit GTGACTATCATCAAGAGCCACACGGGTGGCTTGTCCTTCATGACTCCTTT 5 0.125 No Hit CTTGAATTATCAACTAAAGCTCTTGAGACACCCTGACGTATCCACCAATA 5 0.125 No Hit GGATGAGTTCAGGGTGTGCAGGAGAAGAGAGAGATGCATAAAAGGGGTCT 5 0.125 No Hit CTCGGCTTTATTCACAGACCTAGTCTTTCTTGTAGTAGGTTTAACCTCTT 5 0.125 No Hit CAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCAC 5 0.125 No Hit ACCAGACAAAAACATGATTCCAGGGACGGCTGGGGGGATTCTCCTGTGGA 5 0.125 No Hit AGCTAACATGATATCATAATAATGCTTATGAACTATGACGGAGTCCCACA 5 0.125 No Hit CCCAACTCTGACTGGTGTTTTTGGTCGGAGCTCTTTAGGCACAGAGCTTT 5 0.125 No Hit CGAAACAAAACAAGTATAGGGTTTGCAATTGTTATGATCATGTGCAATTA 5 0.125 No Hit CCCATCTTGGCAAAGACAAGTTCACACTGGAAGGATTTTCCTTGGCCTTT 5 0.125 No Hit GCGGTCATCACCAGTTGCAATTCCTCAGCCACTTTGAGTAGTAATAAGCA 5 0.125 No Hit ACACAGCTCACTGCAGATAATCCCTTGCAAGTCACTTTGACCATCTCTGT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.0875 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.125 0.0 0.0 0.0 0.0 82-83 0.125 0.0 0.0 0.0 0.0 84-85 0.1875 0.0 0.0 0.0 0.0 86-87 0.25 0.0 0.0 0.0 0.0 88-89 0.3125 0.0 0.0 0.0 0.0 90-91 0.3625 0.0 0.0 0.0 0.0 92-93 0.5625 0.0 0.0 0.0 0.0 94-95 0.6625000000000001 0.0 0.0 0.0 0.0 96-97 0.925 0.0 0.0 0.0 0.0 98-99 1.0499999999999998 0.0 0.0 0.0 0.0 100-101 1.125 0.0 0.0 0.0 0.0 102-103 1.3125 0.0 0.0 0.0 0.0 104-105 1.625 0.0 0.0 0.0 0.0 106-107 1.9 0.0 0.0 0.0 0.0 108-109 2.175 0.0 0.0 0.0 0.0 110-111 2.4000000000000004 0.0 0.0 0.0 0.0 112-113 2.6875 0.0 0.0 0.0 0.0 114-115 2.9 0.0 0.0 0.0 0.0 116-117 3.1875 0.0 0.0 0.0 0.0 118-119 3.3499999999999996 0.0 0.0 0.0 0.0 120-121 3.675 0.0 0.0 0.0 0.0 122-123 3.9125 0.0 0.0 0.0 0.0 124-125 4.2375 0.0 0.0 0.0 0.0 126-127 4.6875 0.0 0.0 0.0 0.0 128-129 5.4375 0.0 0.0 0.0 0.0 130-131 6.0125 0.0 0.0 0.0 0.0 132-133 6.65 0.0 0.0 0.0 0.0 134-135 7.175000000000001 0.0 0.0 0.0 0.0 136-137 7.7 0.0 0.0 0.0 0.0 138-139 8.2375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGTTTT 10 0.006830828 145.0 8 CCTGTGG 10 0.006830828 145.0 2 GGATCGG 10 0.006830828 145.0 4 GGATTCT 10 0.006830828 145.0 9 TCGGATT 10 0.006830828 145.0 7 GAGTCTC 10 0.006830828 145.0 145 ATCGGAT 10 0.006830828 145.0 6 CCCTGTG 10 0.006830828 145.0 1 CTGTGGT 10 0.006830828 145.0 3 TGGTGTT 10 0.006830828 145.0 6 CAGGATC 10 0.006830828 145.0 2 GCTCATT 10 0.006830828 145.0 1 CCAGGAT 10 0.006830828 145.0 1 TTGAAAA 20 0.00593511 29.0 120-124 TGAAAAC 20 0.00593511 29.0 120-124 >>END_MODULE SRR13695438 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695438_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.06575 37.0 37.0 37.0 37.0 37.0 2 36.1395 37.0 37.0 37.0 37.0 37.0 3 36.143 37.0 37.0 37.0 37.0 37.0 4 36.2485 37.0 37.0 37.0 37.0 37.0 5 36.3005 37.0 37.0 37.0 37.0 37.0 6 36.299 37.0 37.0 37.0 37.0 37.0 7 36.214 37.0 37.0 37.0 37.0 37.0 8 36.1825 37.0 37.0 37.0 37.0 37.0 9 36.2645 37.0 37.0 37.0 37.0 37.0 10-14 36.2211 37.0 37.0 37.0 37.0 37.0 15-19 36.217600000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.206050000000005 37.0 37.0 37.0 37.0 37.0 25-29 36.08385 37.0 37.0 37.0 37.0 37.0 30-34 36.099900000000005 37.0 37.0 37.0 37.0 37.0 35-39 36.113249999999994 37.0 37.0 37.0 37.0 37.0 40-44 36.144549999999995 37.0 37.0 37.0 37.0 37.0 45-49 36.0862 37.0 37.0 37.0 37.0 37.0 50-54 35.9922 37.0 37.0 37.0 37.0 37.0 55-59 36.05975 37.0 37.0 37.0 37.0 37.0 60-64 35.988749999999996 37.0 37.0 37.0 37.0 37.0 65-69 35.9408 37.0 37.0 37.0 37.0 37.0 70-74 35.93775 37.0 37.0 37.0 37.0 37.0 75-79 35.88965 37.0 37.0 37.0 37.0 37.0 80-84 35.868700000000004 37.0 37.0 37.0 37.0 37.0 85-89 35.7496 37.0 37.0 37.0 37.0 37.0 90-94 35.78545 37.0 37.0 37.0 37.0 37.0 95-99 35.7897 37.0 37.0 37.0 37.0 37.0 100-104 35.771950000000004 37.0 37.0 37.0 37.0 37.0 105-109 35.719500000000004 37.0 37.0 37.0 37.0 37.0 110-114 35.65105 37.0 37.0 37.0 37.0 37.0 115-119 35.63095 37.0 37.0 37.0 37.0 37.0 120-124 35.55275 37.0 37.0 37.0 37.0 37.0 125-129 35.5954 37.0 37.0 37.0 37.0 37.0 130-134 35.46464999999999 37.0 37.0 37.0 37.0 37.0 135-139 35.43435 37.0 37.0 37.0 37.0 37.0 140-144 35.29835 37.0 37.0 37.0 32.2 37.0 145-149 35.157050000000005 37.0 37.0 37.0 27.4 37.0 150-151 34.91525 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 1.0 14 0.0 15 2.0 16 5.0 17 3.0 18 1.0 19 2.0 20 1.0 21 4.0 22 7.0 23 4.0 24 3.0 25 5.0 26 5.0 27 11.0 28 14.0 29 19.0 30 34.0 31 43.0 32 51.0 33 105.0 34 220.0 35 562.0 36 2706.0 37 191.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.67001254705144 20.250941028858218 12.8732747804266 28.20577164366374 2 27.6 25.924999999999997 29.375 17.1 3 22.975 27.150000000000002 28.075 21.8 4 24.6 33.300000000000004 23.9 18.2 5 27.250000000000004 35.175 21.5 16.075 6 21.25 38.6 22.425 17.724999999999998 7 20.150000000000002 22.55 39.175 18.125 8 20.424999999999997 26.400000000000002 28.225 24.95 9 21.125 24.9 31.05 22.925 10-14 23.5 29.110000000000003 27.150000000000002 20.24 15-19 24.125 28.325 27.834999999999997 19.715 20-24 23.90119505975299 28.361418070903543 27.476373818690934 20.261013050652533 25-29 23.80595148787197 27.991997999499873 28.02200550137534 20.180045011252815 30-34 23.217321732173218 28.06780678067807 28.57285728572857 20.142014201420142 35-39 23.14615730786539 28.606430321516076 28.0114005700285 20.236011800590028 40-44 23.61854278141721 27.909186377956697 28.519277891683753 19.95299294894234 45-49 23.17231723172317 28.997899789978998 27.53775377537754 20.292029202920293 50-54 22.485 29.375 27.63 20.51 55-59 23.28582145536384 27.836959239809957 27.82195548887222 21.05526381595399 60-64 23.236161808090404 28.431421571078552 28.14140707035352 20.191009550477524 65-69 23.355 27.01 28.835 20.8 70-74 22.93573393348337 28.51712928232058 27.976994248562143 20.57014253563391 75-79 23.778566785017752 28.194229134370158 28.059208881332196 19.96799519927989 80-84 24.025 27.92 27.529999999999998 20.525 85-89 23.044608921784356 28.640728145629126 27.560512102420482 20.754150830166033 90-94 23.276163808190407 28.21641082054103 28.86644332216611 19.640982049102455 95-99 23.615 28.000000000000004 27.405 20.979999999999997 100-104 22.740685171292824 28.327081770442607 28.172043010752688 20.760190047511877 105-109 23.364672934586917 28.410682136427283 27.735547109421884 20.489097819563913 110-114 23.891194559727985 27.936396819840994 27.986399319965997 20.186009300465024 115-119 23.648547282092313 27.81417212581887 28.42926438965845 20.108016202430363 120-124 23.68592148037009 27.671917979494875 28.262065516379092 20.38009502375594 125-129 24.384876975395077 28.480696139227845 27.190438087617526 19.943988797759552 130-134 24.76119029757439 27.881970492623154 27.696924231057764 19.659914978744688 135-139 25.041260315078766 27.576894223555886 27.58689672418104 19.794948737184296 140-144 25.51627581379069 27.541377068853446 27.2063603180159 19.735986799339965 145-149 26.0865216304076 27.461865466366593 27.501875468867215 18.94973743435859 150-151 26.144036009002253 27.319329832458116 27.219304826206553 19.317329332333085 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 1.5 12 1.5 13 0.5 14 1.0 15 2.0 16 2.0 17 1.0 18 0.0 19 0.5 20 1.5 21 1.0 22 2.0 23 2.5 24 4.0 25 6.0 26 9.0 27 11.0 28 10.5 29 16.0 30 24.0 31 23.0 32 24.0 33 43.0 34 59.0 35 75.0 36 85.5 37 106.0 38 158.0 39 172.5 40 190.5 41 228.0 42 245.5 43 253.5 44 256.5 45 273.0 46 264.5 47 237.5 48 217.0 49 214.5 50 183.5 51 122.5 52 98.0 53 85.5 54 66.0 55 47.5 56 44.0 57 43.0 58 25.5 59 13.5 60 14.5 61 9.5 62 3.5 63 2.5 64 0.5 65 1.0 66 1.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.375 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.005 25-29 0.025 30-34 0.01 35-39 0.005 40-44 0.015 45-49 0.01 50-54 0.0 55-59 0.025 60-64 0.005 65-69 0.0 70-74 0.025 75-79 0.015 80-84 0.0 85-89 0.02 90-94 0.005 95-99 0.0 100-104 0.025 105-109 0.02 110-114 0.005 115-119 0.015 120-124 0.025 125-129 0.02 130-134 0.025 135-139 0.025 140-144 0.005 145-149 0.025 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.325 #Duplication Level Percentage of deduplicated Percentage of total 1 70.91108671789243 48.449999999999996 2 18.807171606293448 25.7 3 6.147091108671789 12.6 4 2.5978777899743872 7.1 5 0.6586169045005488 2.25 6 0.5854372484449323 2.4 7 0.18294914013904134 0.8750000000000001 8 0.07317965605561653 0.4 9 0.03658982802780827 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 9 0.22499999999999998 No Hit TGATAGTCATGCTGGTAACCACTTTGCTAATGATTCTAGTCATGTTATTG 8 0.2 No Hit GTCCTGAAACTCTAAGAGACCCCAGAGGTTTTGCTGTGAAATTCTACACT 8 0.2 No Hit AAAGAATTAAAATCTTTATGGAGCTGCAAGAAATCACCTTATATGCCCTT 7 0.17500000000000002 No Hit CAAGATGATATCCTGGTGACAGAAGGAAGGAAGAGCAAGAATTGGGAGAA 7 0.17500000000000002 No Hit CAAGCACTCGTGTCATCATCTCTTACCTCTTCAGTGGAGACTGCTAGGAA 7 0.17500000000000002 No Hit CAGAGGTATTAGCAACGCTGGAGAACATTGAATCCCCAAAAGGTGCTGCA 7 0.17500000000000002 No Hit GTAGATCCTAATTTAGACCCAGAGCTGGCTCTTGCCCTTAGAGTTTCTAT 7 0.17500000000000002 No Hit ACTTGCTCTGAAGCTTGGTGGATCGAGGGGGTCAATGTTTCGAGCAAGGC 6 0.15 No Hit CATTTTGATCAACTCCTGCTGTTTAATTTCTTGATTGGAGCTTTATCGAA 6 0.15 No Hit CTAGAGAGCGTTTCACCCTTGATGAGCAGCTAAGTCAATCTGTGATTGAG 6 0.15 No Hit ATCCAAGTCTCCAGATGCTAAGGAAATCCCAGTTGACAAAGGTGACCCTG 6 0.15 No Hit CGCCCTGAGAACAACCCGTGGCATGGAGTGGATGAGTGGGCACTGAAGGA 6 0.15 No Hit CCCTATAGGCTCCTTCCTTTTTGGCTACTCGGCAGCACTTCTTTATCACA 6 0.15 No Hit GGACAAGACAATGGATGAGATCAATGAGCAGACAGAGAACATGAAACAGA 6 0.15 No Hit CAAAACCAAAGGCAGTGAAGCACACTCTATTCGTGAAGTTCAAAGATGAC 6 0.15 No Hit GTTGGAACTAGATAATCAGTGTTCTTGTACTTGTTTCAGACTGGTTGATA 6 0.15 No Hit ACCACATATAGAGGGTGTAATAGCTAAGTAGCCTGTAAGAGATGGCTTCC 6 0.15 No Hit CCCATTTCGGATCTACTCTTCTTCTGCTACAGCTACAACTACTACTATAT 6 0.15 No Hit ACTAAGTACGCTGGAGTTGGGGCAGCAGTAGAGTATGCAGTTTTGCATCT 6 0.15 No Hit CATCAATGGTCTCCCTGGAGTAGGAGAGGGTAATGACCTGTACCCTGGTG 6 0.15 No Hit CTGACATTAAGGAAGCCAAGCAGATGTCTCAGCCCGTAATTATACAAAGG 6 0.15 No Hit GACTAATTGTCAGAAGGAAGTTCTCTTCCTTGGAGAACTTGAAGAAGTGC 6 0.15 No Hit GTGCTGAAGGAGTTCCGAAGAGGCTGACCTATGAAGAAATCCAGAGCAAG 6 0.15 No Hit CTGAGGTTTTCTACTACATGGCAGAGAACAATGTCATGTTTGAGGGTATC 5 0.125 No Hit CTCAGAAGCACAGATTTCAAATTCTCAGGGCACGGGTTTGATTGGAGAAG 5 0.125 No Hit GCATATGCCACAAACTTCGTTCCCGGAAAGTGAGCAACAAAAGAGTTTTT 5 0.125 No Hit AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 5 0.125 No Hit CTCGGGAGCAAGTGCTGAAGGAGTTCCGAAGAGGCTGACCTATGAAGAAA 5 0.125 No Hit GTACCAGCCACTGCAGCATCCATCCTTTGTGCATTTTTCAGTTTCCAAGT 5 0.125 No Hit CCTTGAGGATTTCTCGTGCTGACTTACAGTCAAAATTGATTCAATGTTCT 5 0.125 No Hit CAGTCAAGATAGTGGGTGGTATCTCTAGAGAGAAGAGTGAGTTAAGCTAT 5 0.125 No Hit GGTTCCTCTGGTGTTGGTGCTTGCTGAAGATGGAGGTCGGGAAGGATATC 5 0.125 No Hit AGGAGGAGTGAGTCGAAAGATGTCGCCGAATCTTGTTAGGGATTCGAGGA 5 0.125 No Hit CACTCAGTGATCCAAAATTACAACCAAGAAACCAAGAGACAGTGTTGTTT 5 0.125 No Hit CTGCAGATGAAGGGAAACTCACTCATTATTTGAATAGCAGAAGCTTGGTT 5 0.125 No Hit GCCTGCCTCGTTACATGATGCAGTCCAGGCTGCTGCTACAGCAGCATTAG 5 0.125 No Hit TCTGTACCACCTCATCAAGAAAGCTGTTGCCATCAGAAAGCATTTGGAAA 5 0.125 No Hit CAGAGGTAATCCTGTCATTAAAATGATCTGTCTATCCTCAATTGTTAATG 5 0.125 No Hit GTCGAGTCCATATGCCATAGCAAAGTACATGGAAGAGAAGCACAAAGCAG 5 0.125 No Hit GGAGGGCTTTTCTGACGAGGAGCAACTGTCACCGTTATCTCGAAAGCCAG 5 0.125 No Hit CTGAGCTCCAGGAGATGATTGTAATGGATCACTGGAGTTACAGATATAAA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.0875 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.1375 0.0 0.0 0.0 0.0 80-81 0.15 0.0 0.0 0.0 0.0 82-83 0.15 0.0 0.0 0.0 0.0 84-85 0.2125 0.0 0.0 0.0 0.0 86-87 0.275 0.0 0.0 0.0 0.0 88-89 0.3375 0.0 0.0 0.0 0.0 90-91 0.3875 0.0 0.0 0.0 0.0 92-93 0.5875 0.0 0.0 0.0 0.0 94-95 0.6875 0.0 0.0 0.0 0.0 96-97 0.95 0.0 0.0 0.0 0.0 98-99 1.0750000000000002 0.0 0.0 0.0 0.0 100-101 1.15 0.0 0.0 0.0 0.0 102-103 1.3375 0.0 0.0 0.0 0.0 104-105 1.5499999999999998 0.0 0.0 0.0 0.0 106-107 1.825 0.0 0.0 0.0 0.0 108-109 2.0999999999999996 0.0 0.0 0.0 0.0 110-111 2.325 0.0 0.0 0.0 0.0 112-113 2.6125 0.0 0.0 0.0 0.0 114-115 2.825 0.0 0.0 0.0 0.0 116-117 3.1125 0.0 0.0 0.0 0.0 118-119 3.2750000000000004 0.0 0.0 0.0 0.0 120-121 3.6125 0.0 0.0 0.0 0.0 122-123 3.8625 0.0 0.0 0.0 0.0 124-125 4.1875 0.0 0.0 0.0 0.0 126-127 4.637499999999999 0.0 0.0 0.0 0.0 128-129 5.387499999999999 0.0 0.0 0.0 0.0 130-131 5.9375 0.0 0.0 0.0 0.0 132-133 6.575 0.0 0.0 0.0 0.0 134-135 7.1 0.0 0.0 0.0 0.0 136-137 7.625 0.0 0.0 0.0 0.0 138-139 8.162500000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCCCGT 10 0.006830828 145.0 3 CCCGTCA 10 0.006830828 145.0 5 TTCTTTC 10 0.006830828 145.0 8 ACGGGAT 10 0.006830828 145.0 145 GATTCCC 10 0.006830828 145.0 1 CCTTATA 10 0.006830828 145.0 8 TCCCGTC 10 0.006830828 145.0 4 ATTCCCG 10 0.006830828 145.0 2 >>END_MODULE Read 1060562 spots for SRR13695438.sra Written 1060562 spots for SRR13695438.sra Read 1060562 spots for SRR13695438.sra Written 1060562 spots for SRR13695438.sra Read 1060562 spots for SRR13695438.sra Written 1060562 spots for SRR13695438.sra Read 1060562 spots for SRR13695438.sra Written 1060562 spots for SRR13695438.sra Read 1060562 spots for SRR13695438.sra Written 1060562 spots for SRR13695438.sra Read 1060562 spots for SRR13695438.sra Written 1060562 spots for SRR13695438.sra Read 1060562 spots for SRR13695438.sra Written 1060562 spots for SRR13695438.sra Read 1060562 spots for SRR13695438.sra Written 1060562 spots for SRR13695438.sra Read 1060562 spots for SRR13695438.sra Written 1060562 spots for SRR13695438.sra Read 1060562 spots for SRR13695438.sra Written 1060562 spots for SRR13695438.sra Read 1060562 spots for SRR13695438.sra Written 1060562 spots for SRR13695438.sra Read 1060572 spots for SRR13695438.sra Written 1060572 spots for SRR13695438.sra Read 1060562 spots for SRR13695438.sra Written 1060562 spots for SRR13695438.sra Read 1060562 spots for SRR13695438.sra Written 1060562 spots for SRR13695438.sra Read 1060562 spots for SRR13695438.sra Written 1060562 spots for SRR13695438.sra Read 1060562 spots for SRR13695438.sra Written 1060562 spots for SRR13695438.sra Read 1060562 spots for SRR13695438.sra Written 1060562 spots for SRR13695438.sra Read 1060562 spots for SRR13695438.sra Written 1060562 spots for SRR13695438.sra Read 1060562 spots for SRR13695438.sra Written 1060562 spots for SRR13695438.sra Read 1060562 spots for SRR13695438.sra Written 1060562 spots for SRR13695438.sra SRR ids: ['SRR13695438.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_jdemn3b0 SRR13695438.sra spots: 21211250 blocks: [[1, 1060562], [1060563, 2121124], [2121125, 3181686], [3181687, 4242248], [4242249, 5302810], [5302811, 6363372], [6363373, 7423934], [7423935, 8484496], [8484497, 9545058], [9545059, 10605620], [10605621, 11666182], [11666183, 12726744], [12726745, 13787306], [13787307, 14847868], [14847869, 15908430], [15908431, 16968992], [16968993, 18029554], [18029555, 19090116], [19090117, 20150678], [20150679, 21211250]] SRR13695438 file size 7186810 SRR13695438 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695438 SRR13695438_1.fastq SRR13695438_2.fastq Input file: SRR13695438_1.fastq Paired file: SRR13695438_2.fastq trimmed: SRR13695438-trimmed-pair1.fastq, SRR13695438-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 02:35:06 2025 >> started Wed Feb 12 02:35:32 2025 >> done (26.031s) 21211250 read pairs processed; of these: 138 ( 0.00%) short read pairs filtered out after trimming by size control 3405 ( 0.02%) empty read pairs filtered out after trimming by size control 21207707 (99.98%) read pairs available; of these: 2523432 (11.90%) trimmed read pairs available after processing 18684275 (88.10%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 7 0.00% 20 1 0.00% 21 8 0.00% 22 1 0.00% 23 2 0.00% 24 3 0.00% 25 3 0.00% 26 3 0.00% 27 6 0.00% 28 3 0.00% 29 2 0.00% 30 3 0.00% 31 8 0.00% 32 7 0.00% 33 4 0.00% 34 8 0.00% 35 7 0.00% 36 15 0.00% 37 6 0.00% 38 15 0.00% 39 12 0.00% 40 16 0.00% 41 25 0.00% 42 28 0.00% 43 31 0.00% 44 32 0.00% 45 31 0.00% 46 38 0.00% 47 45 0.00% 48 69 0.00% 49 97 0.00% 50 82 0.00% 51 120 0.00% 52 114 0.00% 53 135 0.00% 54 132 0.00% 55 154 0.00% 56 135 0.00% 57 224 0.00% 58 243 0.00% 59 278 0.00% 60 409 0.00% 61 377 0.00% 62 471 0.00% 63 505 0.00% 64 600 0.00% 65 619 0.00% 66 628 0.00% 67 749 0.00% 68 975 0.00% 69 1134 0.01% 70 1284 0.01% 71 1461 0.01% 72 1724 0.01% 73 1935 0.01% 74 2053 0.01% 75 2334 0.01% 76 2541 0.01% 77 2976 0.01% 78 3291 0.02% 79 3449 0.02% 80 4135 0.02% 81 4427 0.02% 82 4987 0.02% 83 5406 0.03% 84 6234 0.03% 85 6978 0.03% 86 7415 0.03% 87 7911 0.04% 88 8346 0.04% 89 8824 0.04% 90 9548 0.05% 91 10842 0.05% 92 11145 0.05% 93 12217 0.06% 94 13112 0.06% 95 14001 0.07% 96 15124 0.07% 97 15385 0.07% 98 16484 0.08% 99 17207 0.08% 100 18751 0.09% 101 19207 0.09% 102 19969 0.09% 103 20688 0.10% 104 22109 0.10% 105 23052 0.11% 106 24349 0.11% 107 24931 0.12% 108 26026 0.12% 109 27478 0.13% 110 28079 0.13% 111 28895 0.14% 112 29936 0.14% 113 30930 0.15% 114 32708 0.15% 115 33919 0.16% 116 34856 0.16% 117 36405 0.17% 118 37511 0.18% 119 37883 0.18% 120 38751 0.18% 121 40447 0.19% 122 42011 0.20% 123 42854 0.20% 124 43815 0.21% 125 45287 0.21% 126 46814 0.22% 127 47490 0.22% 128 48650 0.23% 129 49211 0.23% 130 50689 0.24% 131 51199 0.24% 132 52448 0.25% 133 53963 0.25% 134 54128 0.26% 135 56046 0.26% 136 57305 0.27% 137 58311 0.27% 138 59476 0.28% 139 61323 0.29% 140 61259 0.29% 141 62895 0.30% 142 63819 0.30% 143 64671 0.30% 144 66134 0.31% 145 67211 0.32% 146 67262 0.32% 147 67663 0.32% 148 70185 0.33% 149 71260 0.34% 150 71776 0.34% 151 18684275 88.10% 21207707 reads passed initial QC criterion=sequence-density sequence-density=0.40 sequence-density-rank=1 fanout-score=2.32 fanout-score-rank=25 prefix-density=0.43 prefix-fanout=2.2 sequence=TGAGATGCCTCAGTGCATCCAAACATGGGTAG criterion=fanout-score sequence-density=0.01 sequence-density-rank=29 fanout-score=416.82 fanout-score-rank=1 prefix-density=0.26 prefix-fanout=17.8 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAG criterion=sequence-density sequence-density=0.77 sequence-density-rank=1 fanout-score=2.07 fanout-score-rank=35 prefix-density=0.76 prefix-fanout=2.1 sequence=CTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAGTGAACAAACAAAAGTGTCCGTGAGTGTCTTTATCTTTGTGTCTTCTTCA criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=55.39 fanout-score-rank=1 prefix-density=0.10 prefix-fanout=6.2 sequence=AAACAAGAGAGGTGGAGATATAGGAGAGCATAACCATGTTAGTCCCATATATTTCCAAGATGAAGGCCTTTCTTATCGCATGCATTCTCTTAGCTACCATCGTCTTCTCTCCCCTGTCCACTTGCACTGCTCGAGAATT SRR13695438 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 02:36:14 Started mapping on | Feb 12 02:36:14 Finished on | Feb 12 02:38:37 Mapping speed, Million of reads per hour | 533.90 Number of input reads | 21207707 Average input read length | 296 UNIQUE READS: Uniquely mapped reads number | 19797776 Uniquely mapped reads % | 93.35% Average mapped length | 294.74 Number of splices: Total | 19431251 Number of splices: Annotated (sjdb) | 19001261 Number of splices: GT/AG | 19038903 Number of splices: GC/AG | 308494 Number of splices: AT/AC | 11705 Number of splices: Non-canonical | 72149 Mismatch rate per base, % | 0.37% Deletion rate per base | 0.03% Deletion average length | 2.78 Insertion rate per base | 0.02% Insertion average length | 2.45 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 478749 % of reads mapped to multiple loci | 2.26% Number of reads mapped to too many loci | 67493 % of reads mapped to too many loci | 0.32% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.95% % of reads unmapped: other | 0.12% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 931454 931454 931454 N_multimapping 478749 478749 478749 N_noFeature 795018 19316622 1087750 N_ambiguous 303249 2019 113628 UnstrandedReadsAssigned:18699509 PositiveStrandReadsAssigned:479135 NegativeStrandReadsAssigned:18596398 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695438 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695438-trimmed-pair1.fastq SRR13695438-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 21,207,707 reads, 18,672,339 reads pseudoaligned [quant] estimated average fragment length: 245.3 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,020 rounds 52401 SRR13695438.ke.tsv 34699 SRR13695438.se.tsv 87100 total ==> SRR13695438.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1773.7 651 17.544 Potri.005G024800.1.v4.1 1035 790.7 512 30.9518 Potri.004G059700.1.v4.1 961 716.807 0 0 Potri.007G009000.2.v4.1 1416 1171.7 0 0 Potri.003G141000.2.v4.1 2943 2698.7 1007.44 17.844 Potri.016G087400.1.v4.1 270 85.5453 1082 604.587 Potri.015G069301.1.v4.1 564 327.704 0 0 Potri.010G195200.1.v4.1 1773 1528.7 123 3.84601 Potri.012G127500.1.v4.1 977 732.752 109 7.11046 ==> SRR13695438.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 214 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 335 Potri.001G212900.v4.1 7 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 16 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 6 SRR13695438 completed mapping pipeline successfully