Starting /dee2/code/volunteer_pipeline.sh SRR13695439 current disk space = 3048881823744 free memory = 1443881976 SRR13695439 SRAfilesize e0e8ccc63b1b6e6ce8ec0f86cd191da8 SRR13695439.sra SRR13695439.sra file validated SRR13695439 is paired end SRR13695439 is conventional basespace SRR13695439 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695439_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.606 37.0 37.0 37.0 37.0 37.0 2 36.34325 37.0 37.0 37.0 37.0 37.0 3 36.6075 37.0 37.0 37.0 37.0 37.0 4 36.5975 37.0 37.0 37.0 37.0 37.0 5 36.6905 37.0 37.0 37.0 37.0 37.0 6 36.6105 37.0 37.0 37.0 37.0 37.0 7 36.5015 37.0 37.0 37.0 37.0 37.0 8 36.5915 37.0 37.0 37.0 37.0 37.0 9 36.483 37.0 37.0 37.0 37.0 37.0 10-14 36.5801 37.0 37.0 37.0 37.0 37.0 15-19 36.5863 37.0 37.0 37.0 37.0 37.0 20-24 36.5249 37.0 37.0 37.0 37.0 37.0 25-29 36.5319 37.0 37.0 37.0 37.0 37.0 30-34 36.4696 37.0 37.0 37.0 37.0 37.0 35-39 36.4338 37.0 37.0 37.0 37.0 37.0 40-44 36.4762 37.0 37.0 37.0 37.0 37.0 45-49 36.440000000000005 37.0 37.0 37.0 37.0 37.0 50-54 36.4066 37.0 37.0 37.0 37.0 37.0 55-59 36.3791 37.0 37.0 37.0 37.0 37.0 60-64 36.4212 37.0 37.0 37.0 37.0 37.0 65-69 36.3448 37.0 37.0 37.0 37.0 37.0 70-74 36.3241 37.0 37.0 37.0 37.0 37.0 75-79 36.347 37.0 37.0 37.0 37.0 37.0 80-84 36.2764 37.0 37.0 37.0 37.0 37.0 85-89 36.2818 37.0 37.0 37.0 37.0 37.0 90-94 36.126799999999996 37.0 37.0 37.0 37.0 37.0 95-99 36.2047 37.0 37.0 37.0 37.0 37.0 100-104 36.138600000000004 37.0 37.0 37.0 37.0 37.0 105-109 36.1471 37.0 37.0 37.0 37.0 37.0 110-114 36.106700000000004 37.0 37.0 37.0 37.0 37.0 115-119 36.0498 37.0 37.0 37.0 37.0 37.0 120-124 36.0099 37.0 37.0 37.0 37.0 37.0 125-129 35.9763 37.0 37.0 37.0 37.0 37.0 130-134 35.941500000000005 37.0 37.0 37.0 37.0 37.0 135-139 35.9066 37.0 37.0 37.0 37.0 37.0 140-144 35.7493 37.0 37.0 37.0 37.0 37.0 145-149 35.5301 37.0 37.0 37.0 37.0 37.0 150-151 35.32625 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 0.0 21 0.0 22 1.0 23 0.0 24 2.0 25 2.0 26 3.0 27 12.0 28 17.0 29 13.0 30 23.0 31 36.0 32 33.0 33 72.0 34 124.0 35 321.0 36 2988.0 37 352.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.324999999999996 12.0 8.625 40.050000000000004 2 19.56248428463666 12.84888106613025 36.35906462157405 31.229570027659037 3 17.724999999999998 18.075 26.6 37.6 4 23.35 24.8 23.525 28.325 5 24.675 29.625 25.124999999999996 20.575 6 19.225 36.825 24.75 19.2 7 15.15 26.8 40.625 17.424999999999997 8 16.5 27.35 32.425 23.724999999999998 9 17.549999999999997 25.724999999999998 32.425 24.3 10-14 19.355 30.39 27.29 22.965 15-19 19.900000000000002 27.955000000000002 28.439999999999998 23.705000000000002 20-24 19.34 28.48 28.4 23.78 25-29 19.755 28.294999999999998 28.645 23.305 30-34 20.145 28.965000000000003 26.840000000000003 24.05 35-39 19.220000000000002 29.37 27.634999999999998 23.775 40-44 19.715 29.575000000000003 27.32 23.39 45-49 19.185 29.17 27.07 24.575 50-54 20.78 28.53 27.77 22.919999999999998 55-59 20.485 28.165000000000003 28.025 23.325000000000003 60-64 19.84 28.15 28.849999999999998 23.16 65-69 21.15 28.439999999999998 27.62 22.79 70-74 19.56 28.425 27.825 24.19 75-79 20.225 28.625 26.455000000000002 24.695 80-84 19.77 29.409999999999997 27.465 23.355 85-89 20.115 27.88 27.82 24.185000000000002 90-94 20.150000000000002 29.26 26.995 23.595 95-99 20.03 28.904999999999998 27.405 23.66 100-104 20.615 28.939999999999998 26.91 23.535 105-109 20.150000000000002 28.825 27.375 23.65 110-114 20.0 29.165000000000003 27.339999999999996 23.494999999999997 115-119 21.47 27.77 27.415 23.345 120-124 21.475 28.325 27.265 22.935 125-129 20.599999999999998 28.335 27.515 23.549999999999997 130-134 20.45 28.044999999999998 28.000000000000004 23.505000000000003 135-139 21.0 28.42 26.51 24.07 140-144 21.48 28.185 26.56 23.775 145-149 21.595 28.1 26.979999999999997 23.325000000000003 150-151 21.637500000000003 27.55 26.5375 24.275 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 0.5 19 2.5 20 2.5 21 0.5 22 0.5 23 2.0 24 2.0 25 0.5 26 1.5 27 3.5 28 13.5 29 18.0 30 20.5 31 34.0 32 37.0 33 40.0 34 59.0 35 78.5 36 95.0 37 114.0 38 141.5 39 163.0 40 180.5 41 209.5 42 234.0 43 243.0 44 261.0 45 262.5 46 268.0 47 265.5 48 225.5 49 191.5 50 157.5 51 146.0 52 124.5 53 95.5 54 81.5 55 63.5 56 51.0 57 36.0 58 24.0 59 18.5 60 13.0 61 8.0 62 3.5 63 1.0 64 0.5 65 0.5 66 1.0 67 1.5 68 0.5 69 0.0 70 0.0 71 0.0 72 0.5 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.575 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.9 #Duplication Level Percentage of deduplicated Percentage of total 1 70.25036818851251 47.699999999999996 2 19.40353460972018 26.35 3 5.8541973490427095 11.924999999999999 4 3.12960235640648 8.5 5 0.8100147275405009 2.75 6 0.14727540500736377 0.6 7 0.22091310751104565 1.05 8 0.11045655375552282 0.6 9 0.036818851251840944 0.22499999999999998 >10 0.036818851251840944 0.3 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAAC 12 0.3 No Hit GGATGCAAAGGGAAGAAGGGCTTCTCATCAAGTAATGCCGAGCTCAACAG 9 0.22499999999999998 No Hit CCGGCATTACATCAAAGCAAAAACCTATATAACCCCTTTCTAGGCCCTAT 8 0.2 No Hit GCACTTCTGTAGGAAAGCCTTCTCAGGGTTCTTTCCATCAATTTCTGAGT 8 0.2 No Hit CTCCTAACCAACTTTGTAACCATCCTCTGCCTCCAGAAAACCCACCAGAA 8 0.2 No Hit GCTTGAACTCAGACTTGGTGATTTTCCAAGAGATCTCCCAGAACCCGAAA 7 0.17500000000000002 No Hit CTCTGTTGCTTCTGCAAGCTCTTGGTAAGTAAAACACCGCAAATTTGTTT 7 0.17500000000000002 No Hit TATGCATGTGCCAGTTCGCCTTGAGTTGAGGTCATGGCTCGATCTCCCTT 7 0.17500000000000002 No Hit CTGGTGACCATCCGTTGCTTTGCCACCAGCTTGTTCCACGAGATAACTCA 7 0.17500000000000002 No Hit CTCATTTTTACTTTCTTCCATGGAGAAATGATCTGTAACTTCAACACACC 7 0.17500000000000002 No Hit TGACATTCTTCAAAGAAAGGTCTTTATCTCTAGCCAGTGCCAGGATTCTT 7 0.17500000000000002 No Hit AGCTCTTCTTTCTTTTCAGGCTCTTTTGCTGGTCCTACTGAGATTATTTC 6 0.15 No Hit CGAATTGATAGAGCATTAAGTGAACATGCCAACAGTTGAACAAAACAAAT 6 0.15 No Hit CCCATATATCACACACCGCCCTTCTACAAGGTACAGGACAATATGGAGGT 6 0.15 No Hit CTTTAACATAATCCCCGTTGCCGGCTAAAAATGTCACGTAGGCCCTCTTA 6 0.15 No Hit ACGAGAAACTATAATTTAAAAAGAGATTTTTATACGGTGCAGAGTTTTTC 5 0.125 No Hit TTTCGATTAATTAATTACCGTAGAGAGCATGTATGCCATCAATATCATCC 5 0.125 No Hit ATTCAAGTAAGAGATCTGTCCAATATTATTTGAAATCATTCTTAGAAGAG 5 0.125 No Hit GCCAAAGTTAAGAACTTGTCGACGCAGTCGGAAGGATAAGAACCCATTCG 5 0.125 No Hit CAAGACCAAACGATAAACCAATCACGATCGCCACTAGTATTACACAAGTG 5 0.125 No Hit ATAAGCTTTGCGGGTTCACCAGCGTTCCCACTTTCCAATTCTCCAGCACT 5 0.125 No Hit TGTGCTTTACCCCTAGATGTCCAGTCAACTGCTGCGCCTCAACGCATTTC 5 0.125 No Hit CATTGGTGTTGAAACCACATGCCACTTCCCCGTCTCTAGACACTGCTATC 5 0.125 No Hit GTAAATGTTGGGTAATTCTGATTCTTCATCCAAACCTTCGCTTCTCCCAT 5 0.125 No Hit ATCACCATAAACCTCGCTGGTACTGGTAAGCAAAAACCTAGCACCAACTC 5 0.125 No Hit AGTAAATATAAATAAGAAGCTATATGGTCTGGACTGCTTTTCTAGAAAGG 5 0.125 No Hit TTCTGATATTGAACAAACCTTATTTGCAACTTTATCAAGAAACCCATCAC 5 0.125 No Hit GTTGTTCGGCACTAAGAGTGGGATCCTTCCTTAGTTTGGATGCTAGAGTT 5 0.125 No Hit CTTGAAGACAACATTTCTTTGCTTGATTAATCAAGATCAGTACATGGATC 5 0.125 No Hit TACGCTTCTAGCAACATCTCCAGCTCTTCTATATTCTCAGTAGCACTTTC 5 0.125 No Hit CGCTAACGCACTTCGAAATTTTATCCAAATTACATTTTTTAACCATTCCT 5 0.125 No Hit ATCCATATCTTAGCAGCTTCACTGCAAGCTCGCATGCCTTCCTCATAATC 5 0.125 No Hit ATCCTTCGATAAATTCAGATCACAGATTGCACGCGCATAGAGGTTCTACT 5 0.125 No Hit CTTCATTAAAACCACACCAGAGGCCACAGACATGGCCAATACATAACAAT 5 0.125 No Hit GCAAGATGTACCCTGACATGAGCTCCTCAGAGACGATCACCAACGAAACT 5 0.125 No Hit CTTTCATTGATTGTGTGGTGGACTTTGATTTCTTCTTTTTAGAGCTTGTT 5 0.125 No Hit TGGTAAACTAATAGAATGCATGTCAGTGGTACATGAGGTAGGAGAAGAGC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0125 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.037500000000000006 0.0 0.0 0.0 0.0 82-83 0.1 0.0 0.0 0.0 0.0 84-85 0.125 0.025 0.0 0.0 0.0 86-87 0.2375 0.025 0.0 0.0 0.0 88-89 0.375 0.025 0.0 0.0 0.0 90-91 0.48750000000000004 0.025 0.0 0.0 0.0 92-93 0.575 0.025 0.0 0.0 0.0 94-95 0.6375 0.025 0.0 0.0 0.0 96-97 0.8125 0.025 0.0 0.0 0.0 98-99 0.9624999999999999 0.025 0.0 0.0 0.0 100-101 1.1 0.025 0.0 0.0 0.0 102-103 1.2875 0.025 0.0 0.0 0.0 104-105 1.425 0.025 0.0 0.0 0.0 106-107 1.625 0.025 0.0 0.0 0.0 108-109 1.7875 0.025 0.0 0.0 0.0 110-111 1.9749999999999999 0.025 0.0 0.0 0.0 112-113 2.2874999999999996 0.025 0.0 0.0 0.0 114-115 2.7 0.025 0.0 0.0 0.0 116-117 2.95 0.025 0.0 0.0 0.0 118-119 3.4375 0.05 0.0 0.0 0.0 120-121 3.875 0.05 0.0 0.0 0.0 122-123 4.225 0.05 0.0 0.0 0.0 124-125 4.512499999999999 0.05 0.0 0.0 0.0 126-127 4.949999999999999 0.05 0.0 0.0 0.0 128-129 5.3375 0.05 0.0 0.0 0.0 130-131 5.85 0.05 0.0 0.0 0.0 132-133 6.5125 0.05 0.0 0.0 0.0 134-135 6.862500000000001 0.05 0.0 0.0 0.0 136-137 7.5875 0.05 0.0 0.0 0.0 138-139 8.399999999999999 0.05 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAAAAAA 20 0.00593511 29.0 100-104 >>END_MODULE SRR13695439 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695439_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.9935 37.0 37.0 37.0 37.0 37.0 2 35.9535 37.0 37.0 37.0 37.0 37.0 3 35.97 37.0 37.0 37.0 37.0 37.0 4 36.112 37.0 37.0 37.0 37.0 37.0 5 36.072 37.0 37.0 37.0 37.0 37.0 6 36.2785 37.0 37.0 37.0 37.0 37.0 7 36.1085 37.0 37.0 37.0 37.0 37.0 8 36.1495 37.0 37.0 37.0 37.0 37.0 9 36.08 37.0 37.0 37.0 37.0 37.0 10-14 36.091 37.0 37.0 37.0 37.0 37.0 15-19 36.0838 37.0 37.0 37.0 37.0 37.0 20-24 36.09725 37.0 37.0 37.0 37.0 37.0 25-29 36.0278 37.0 37.0 37.0 37.0 37.0 30-34 36.023199999999996 37.0 37.0 37.0 37.0 37.0 35-39 35.981100000000005 37.0 37.0 37.0 37.0 37.0 40-44 35.9879 37.0 37.0 37.0 37.0 37.0 45-49 35.96374999999999 37.0 37.0 37.0 37.0 37.0 50-54 35.961400000000005 37.0 37.0 37.0 37.0 37.0 55-59 35.837399999999995 37.0 37.0 37.0 37.0 37.0 60-64 35.8193 37.0 37.0 37.0 37.0 37.0 65-69 35.798950000000005 37.0 37.0 37.0 37.0 37.0 70-74 35.6788 37.0 37.0 37.0 37.0 37.0 75-79 35.7526 37.0 37.0 37.0 37.0 37.0 80-84 35.76735 37.0 37.0 37.0 37.0 37.0 85-89 35.60485 37.0 37.0 37.0 37.0 37.0 90-94 35.6238 37.0 37.0 37.0 37.0 37.0 95-99 35.61795 37.0 37.0 37.0 37.0 37.0 100-104 35.63609999999999 37.0 37.0 37.0 37.0 37.0 105-109 35.510600000000004 37.0 37.0 37.0 37.0 37.0 110-114 35.5008 37.0 37.0 37.0 37.0 37.0 115-119 35.4815 37.0 37.0 37.0 37.0 37.0 120-124 35.37859999999999 37.0 37.0 37.0 37.0 37.0 125-129 35.509449999999994 37.0 37.0 37.0 37.0 37.0 130-134 35.2565 37.0 37.0 37.0 32.2 37.0 135-139 35.222899999999996 37.0 37.0 37.0 32.2 37.0 140-144 35.1072 37.0 37.0 37.0 27.4 37.0 145-149 34.972500000000004 37.0 37.0 37.0 25.0 37.0 150-151 34.70425 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 2.0 14 1.0 15 0.0 16 3.0 17 1.0 18 1.0 19 4.0 20 2.0 21 5.0 22 2.0 23 6.0 24 8.0 25 14.0 26 8.0 27 18.0 28 13.0 29 24.0 30 25.0 31 43.0 32 79.0 33 110.0 34 250.0 35 710.0 36 2530.0 37 140.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.5529352734571 22.428499749121926 12.067235323632715 28.95132965378826 2 27.1 25.624999999999996 30.425 16.85 3 20.025000000000002 28.425 31.474999999999998 20.075000000000003 4 24.325 33.5 22.725 19.45 5 25.974999999999998 35.375 23.5 15.15 6 22.2 38.1 22.8 16.900000000000002 7 20.200000000000003 21.475 36.675000000000004 21.65 8 20.4 28.175 28.749999999999996 22.675 9 22.375 25.474999999999998 29.325000000000003 22.825 10-14 23.085 29.695 26.6 20.62 15-19 22.775000000000002 27.834999999999997 28.139999999999997 21.25 20-24 23.20080020005001 28.997249312328083 27.506876719179797 20.29507376844211 25-29 23.53176588294147 27.943971985993 28.194097048524263 20.33016508254127 30-34 23.289315726290518 28.61144457783113 27.631052420968388 20.468187274909962 35-39 22.616785035510652 28.46854056216865 28.22846854056217 20.686205861758527 40-44 22.769107643057225 27.490996398559425 28.626450580232092 21.113445378151262 45-49 22.72795478417446 28.85009753413695 27.91977192017206 20.50217576151653 50-54 21.991597479243772 28.808642592777833 28.13844153245974 21.061318395518654 55-59 22.81640820410205 28.194097048524263 28.059029514757377 20.930465232616307 60-64 23.131939581874562 27.47324197259178 28.413524057217167 20.981294388316496 65-69 22.64339650947642 26.734010101515228 29.169375406310948 21.453217982697403 70-74 22.89144572286143 27.2736368184092 28.3591795897949 21.475737868934466 75-79 22.699079631852744 28.171268507402964 27.09083633453381 22.038815526210485 80-84 23.50587646911728 28.11702925731433 27.726931732933235 20.650162540635158 85-89 23.670651793306988 28.112650692811762 26.842078935520984 21.374618578360263 90-94 23.10193057917375 28.44853456036811 27.63829148744623 20.811243373011905 95-99 23.415853963490875 27.826956739184794 28.347086771692926 20.41010252563141 100-104 23.66183091545773 28.114057028514257 26.768384192096047 21.455727863931966 105-109 22.756378189094548 28.149074537268636 28.224112056028016 20.870435217608804 110-114 23.221966589976994 28.39351805541662 27.858357507252173 20.526157847354206 115-119 24.307153576788394 28.554277138569283 27.173586793396698 19.96498249124562 120-124 23.871935967983994 28.52426213106553 28.319159579789893 19.28464232116058 125-129 23.89575308889 28.687909559301684 27.217247761492676 20.19908959031564 130-134 25.27263631815908 27.48874437218609 27.368684342171086 19.86993496748374 135-139 24.267133566783393 28.22911455727864 27.61880940470235 19.884942471235618 140-144 25.35260578173452 28.983695108532558 26.182854856456938 19.480844253275983 145-149 26.013006503251624 28.07903951975988 26.678339169584792 19.229614807403703 150-151 27.126063031515756 26.18809404702351 26.80090045022511 19.884942471235618 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.0 5 1.0 6 1.0 7 0.0 8 0.5 9 0.5 10 0.5 11 0.5 12 0.5 13 0.5 14 1.0 15 1.5 16 0.5 17 0.0 18 1.0 19 1.0 20 0.5 21 0.5 22 1.0 23 3.0 24 2.5 25 6.0 26 6.0 27 2.5 28 5.0 29 7.5 30 15.5 31 20.5 32 32.5 33 51.5 34 52.5 35 70.0 36 113.5 37 135.5 38 139.0 39 159.0 40 196.0 41 224.0 42 232.5 43 251.0 44 260.5 45 279.0 46 276.5 47 234.5 48 227.5 49 188.5 50 134.0 51 129.0 52 123.5 53 90.0 54 69.0 55 65.5 56 49.5 57 38.5 58 29.5 59 19.0 60 14.5 61 9.5 62 8.0 63 5.0 64 0.5 65 0.0 66 0.0 67 0.0 68 0.5 69 1.0 70 0.5 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.5 77 1.5 78 1.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 1.0 98 0.5 99 0.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35000000000000003 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.025 25-29 0.05 30-34 0.04 35-39 0.03 40-44 0.04 45-49 0.034999999999999996 50-54 0.03 55-59 0.05 60-64 0.03 65-69 0.015 70-74 0.05 75-79 0.04 80-84 0.025 85-89 0.045 90-94 0.03 95-99 0.025 100-104 0.05 105-109 0.05 110-114 0.03 115-119 0.05 120-124 0.05 125-129 0.045 130-134 0.05 135-139 0.05 140-144 0.03 145-149 0.05 150-151 0.05 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.77499999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 71.68302435478007 49.3 2 18.647764449291166 25.650000000000002 3 5.634314794620138 11.625 4 2.6172300981461287 7.199999999999999 5 0.7270083605961468 2.5 6 0.21810250817884408 0.8999999999999999 7 0.21810250817884408 1.05 8 0.14540167211922936 0.8 9 0.03635041802980734 0.22499999999999998 >10 0.07270083605961468 0.75 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGAACACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATC 16 0.4 No Hit CACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATC 14 0.35000000000000003 No Hit GAGGAGGGTCATAGTAATGTCACTCTACTGGCTTCTCCGGCCTCAGCAGA 9 0.22499999999999998 No Hit CCTCAACAAGCCCCTCTTAGACAGAATAGTGTCGGTGGTATTCTTTGCAA 8 0.2 No Hit CTTGCAGCCTTTCACTGAAGAAAAGTGCAGACAGTCTTGCTTGAATGATT 8 0.2 No Hit CCTAACCCCAGAAGGTAATGCAGTGGTCCATGAAGAAGTAGTAAGAGTGT 8 0.2 No Hit AAGAACCAGATGGTTTCGGTCCCTTTGGAAGAACTGCCAAGCAAATTGAA 8 0.2 No Hit GTTGCTTTCTTTGGAGGTGGAAAACGAGAAAAAGCTATGATTGAGTCAAG 7 0.17500000000000002 No Hit GTTAGTTTCAAGTTCACTCCCAACTCAGAAGGGTTTCTGGTTGGGAGTGA 7 0.17500000000000002 No Hit AAAAACTCTAACTGCAATAATGTGTCTCCAAATGAGATATCAGGAACTAC 7 0.17500000000000002 No Hit GAAAGACTGCTGTTTTTGTTCTGTCTACTCTTCAGCAAATTGAGCCTACC 7 0.17500000000000002 No Hit CAACGGGAAGCCTTACTCTGGACGTTATATAGGCTGCCTTGTTGGGGAAA 7 0.17500000000000002 No Hit AGAGTAGCATCCTTAGCGTTGCTGGTTTACTTTCCTAACAATCCTCAAAA 7 0.17500000000000002 No Hit CTTTCACACCACATGTTCGAAAACTTCCAGTAGTTCTCAAAAATGTCCAT 6 0.15 No Hit AGGGAGCACTGCATAGCTTATAAGCTTGTAAGAGATGGCTTCCTCCTCTA 6 0.15 No Hit AGACAATATTGATTTGCTTAAGGGCGCTTGTGGCAAGTCACTTGCTTCTT 6 0.15 No Hit TGTTGATCTTGCATCCCTTCGTGACCCGCAGGAGGATGCTGCCAGGGTCA 6 0.15 No Hit CAAACAGAACCAGAAACAAAACAAGAACCAAAACCATGCACATGTTGTGA 6 0.15 No Hit GAAGAAAGCTGTGTTGAAATTGGGTTTGCACGATGAGAAAAGCAAGAAAA 6 0.15 No Hit GCATCGCCTGTTCATTACAAGCACAACCCGGTCAAGACAATAAAGACTAA 5 0.125 No Hit AGAGAACACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 5 0.125 No Hit CTGCATAGCTTATAAGCTTGTAAGAGATGGCTTCCTCCTCTATGATCTCA 5 0.125 No Hit GCTGAACTCATTTGGGAGTGACAATATTCCCAAGGTGAGGAAGCCTTACA 5 0.125 No Hit CTTTATTTAGGCTGAATAGTCAACGTGATTTATTTTTAACTTTCTTTTTT 5 0.125 No Hit CTCTTCTCTATCTCCCCTTCTCCTTGTCTTCCATTTGGGCGACGTTTGTT 5 0.125 No Hit GCCGAACTCGAATCAATCGCCTCTGCAGGTGCTGTAAAGGGTGACAGATA 5 0.125 No Hit TGGAACTCACAACCCCGTTTTGAATTCTTATGAGTATCTCAGTCAAGGTC 5 0.125 No Hit CAATTTGGAAGCTGAAGGTGATGATAGCTTGGTGGTGACTTTGGTGCCTA 5 0.125 No Hit CAAATGATGGTGGGACATCTATTTCTGCTCCGGTTTCTCCTGTTTCATCA 5 0.125 No Hit TGTATGTAGTCTTGCATTTGTCTTGCCATCCTTTTTCTCCTCAAGGAAAA 5 0.125 No Hit AGATGAAACGATACAAATTTTGAAAGGACTTCGTGAGCGATATGAGATCC 5 0.125 No Hit CCTAAGCAAAGAACAACTTCGTATTTAGTTCATCCATTTGCTTCATCAAT 5 0.125 No Hit GGGATGGTTATCCTTTTGATGGACCTGGGAAAATACTAGCCCATGCTTTT 5 0.125 No Hit CTTCCGCGGGTGATCTCTTGACTCTAGATTTTGATGCAGAAAGTGAGTAT 5 0.125 No Hit ACCAATCTCGCACGGCAAAAACATTGTTAGAGAGGTTAATATCGCATATC 5 0.125 No Hit GAAATAACAGAGTTCTAGACATGCTTGTTGGGGATATCTATGGTGGGATG 5 0.125 No Hit CTCATAGGCAGTGGCTTGGTTAAGGGAACCCACCGGAGCCGTAGCGAAAG 5 0.125 No Hit TGGAACCTGAGGAATTATTTGAAGTTGTGTCCCAAGCATTACTAGCATCA 5 0.125 No Hit GTAATTGTAGTCTCTGCTCTCTCCTCACTCCTCTTCTTTCTCTCTTCTGT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0125 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.037500000000000006 0.0 0.0 0.0 0.0 82-83 0.1 0.0 0.0 0.0 0.0 84-85 0.125 0.0 0.0 0.0 0.0 86-87 0.21250000000000002 0.0 0.0 0.0 0.0 88-89 0.35 0.0 0.0 0.0 0.0 90-91 0.48750000000000004 0.0 0.0 0.0 0.0 92-93 0.575 0.0 0.0 0.0 0.0 94-95 0.6375 0.0 0.0 0.0 0.0 96-97 0.8125 0.0 0.0 0.0 0.0 98-99 0.9624999999999999 0.0 0.0 0.0 0.0 100-101 1.1 0.0 0.0 0.0 0.0 102-103 1.25 0.0 0.0 0.0 0.0 104-105 1.375 0.0 0.0 0.0 0.0 106-107 1.5625 0.0 0.0 0.0 0.0 108-109 1.7125 0.0 0.0 0.0 0.0 110-111 1.9 0.0 0.0 0.0 0.0 112-113 2.2375 0.0 0.0 0.0 0.0 114-115 2.675 0.0 0.0 0.0 0.0 116-117 2.925 0.0 0.0 0.0 0.0 118-119 3.4124999999999996 0.0 0.0 0.0 0.0 120-121 3.8375 0.0 0.0 0.0 0.0 122-123 4.175 0.0 0.0 0.0 0.0 124-125 4.4625 0.0 0.0 0.0 0.0 126-127 4.9 0.0 0.0 0.0 0.0 128-129 5.2875 0.0 0.0 0.0 0.0 130-131 5.8 0.0 0.0 0.0 0.0 132-133 6.4375 0.0 0.0 0.0 0.0 134-135 6.8 0.0 0.0 0.0 0.0 136-137 7.525 0.0 0.0 0.0 0.0 138-139 8.325 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTGTGT 10 0.0070686177 143.35 8 CTGTGTT 10 0.0070686177 143.35 9 AAAGCTG 10 0.0070686177 143.35 5 AGCTGTG 10 0.0070686177 143.35 7 >>END_MODULE Read 932501 spots for SRR13695439.sra Written 932501 spots for SRR13695439.sra Read 932501 spots for SRR13695439.sra Written 932501 spots for SRR13695439.sra Read 932501 spots for SRR13695439.sra Written 932501 spots for SRR13695439.sra Read 932501 spots for SRR13695439.sra Written 932501 spots for SRR13695439.sra Read 932501 spots for SRR13695439.sra Written 932501 spots for SRR13695439.sra Read 932501 spots for SRR13695439.sra Written 932501 spots for SRR13695439.sra Read 932501 spots for SRR13695439.sra Written 932501 spots for SRR13695439.sra Read 932501 spots for SRR13695439.sra Written 932501 spots for SRR13695439.sra Read 932501 spots for SRR13695439.sra Written 932501 spots for SRR13695439.sra Read 932501 spots for SRR13695439.sra Written 932501 spots for SRR13695439.sra Read 932501 spots for SRR13695439.sra Written 932501 spots for SRR13695439.sra Read 932501 spots for SRR13695439.sra Written 932501 spots for SRR13695439.sra Read 932501 spots for SRR13695439.sra Written 932501 spots for SRR13695439.sra Read 932501 spots for SRR13695439.sra Written 932501 spots for SRR13695439.sra Read 932501 spots for SRR13695439.sra Written 932501 spots for SRR13695439.sra Read 932501 spots for SRR13695439.sra Written 932501 spots for SRR13695439.sra Read 932501 spots for SRR13695439.sra Written 932501 spots for SRR13695439.sra Read 932507 spots for SRR13695439.sra Written 932507 spots for SRR13695439.sra Read 932501 spots for SRR13695439.sra Written 932501 spots for SRR13695439.sra Read 932501 spots for SRR13695439.sra Written 932501 spots for SRR13695439.sra SRR ids: ['SRR13695439.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_nu4n8f_5 SRR13695439.sra spots: 18650026 blocks: [[1, 932501], [932502, 1865002], [1865003, 2797503], [2797504, 3730004], [3730005, 4662505], [4662506, 5595006], [5595007, 6527507], [6527508, 7460008], [7460009, 8392509], [8392510, 9325010], [9325011, 10257511], [10257512, 11190012], [11190013, 12122513], [12122514, 13055014], [13055015, 13987515], [13987516, 14920016], [14920017, 15852517], [15852518, 16785018], [16785019, 17717519], [17717520, 18650026]] SRR13695439 file size 6316394 SRR13695439 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695439 SRR13695439_1.fastq SRR13695439_2.fastq Input file: SRR13695439_1.fastq Paired file: SRR13695439_2.fastq trimmed: SRR13695439-trimmed-pair1.fastq, SRR13695439-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 02:58:00 2025 >> started Wed Feb 12 02:58:22 2025 >> done (22.848s) 18650026 read pairs processed; of these: 116 ( 0.00%) short read pairs filtered out after trimming by size control 2102 ( 0.01%) empty read pairs filtered out after trimming by size control 18647808 (99.99%) read pairs available; of these: 1977165 (10.60%) trimmed read pairs available after processing 16670643 (89.40%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 1 0.00% 20 3 0.00% 21 3 0.00% 22 0 0.00% 23 2 0.00% 24 2 0.00% 25 3 0.00% 26 2 0.00% 27 2 0.00% 28 1 0.00% 29 3 0.00% 30 3 0.00% 31 4 0.00% 32 1 0.00% 33 1 0.00% 34 0 0.00% 35 6 0.00% 36 2 0.00% 37 18 0.00% 38 8 0.00% 39 12 0.00% 40 11 0.00% 41 15 0.00% 42 8 0.00% 43 9 0.00% 44 6 0.00% 45 22 0.00% 46 27 0.00% 47 20 0.00% 48 31 0.00% 49 45 0.00% 50 28 0.00% 51 62 0.00% 52 55 0.00% 53 64 0.00% 54 61 0.00% 55 73 0.00% 56 74 0.00% 57 93 0.00% 58 151 0.00% 59 125 0.00% 60 178 0.00% 61 213 0.00% 62 225 0.00% 63 260 0.00% 64 268 0.00% 65 300 0.00% 66 318 0.00% 67 411 0.00% 68 542 0.00% 69 574 0.00% 70 646 0.00% 71 762 0.00% 72 859 0.00% 73 1089 0.01% 74 1188 0.01% 75 1325 0.01% 76 1447 0.01% 77 1711 0.01% 78 1878 0.01% 79 2088 0.01% 80 2264 0.01% 81 2705 0.01% 82 3129 0.02% 83 3433 0.02% 84 4028 0.02% 85 4545 0.02% 86 4679 0.03% 87 5076 0.03% 88 5586 0.03% 89 6080 0.03% 90 6513 0.03% 91 7184 0.04% 92 7698 0.04% 93 8559 0.05% 94 9416 0.05% 95 10037 0.05% 96 10634 0.06% 97 11478 0.06% 98 11955 0.06% 99 12495 0.07% 100 13446 0.07% 101 13811 0.07% 102 14831 0.08% 103 15428 0.08% 104 16429 0.09% 105 17370 0.09% 106 18111 0.10% 107 19289 0.10% 108 19964 0.11% 109 21143 0.11% 110 21098 0.11% 111 22223 0.12% 112 22911 0.12% 113 23782 0.13% 114 25115 0.13% 115 25857 0.14% 116 26993 0.14% 117 27880 0.15% 118 29263 0.16% 119 29941 0.16% 120 31003 0.17% 121 31770 0.17% 122 32018 0.17% 123 33643 0.18% 124 34968 0.19% 125 35680 0.19% 126 36907 0.20% 127 38395 0.21% 128 39163 0.21% 129 39405 0.21% 130 40496 0.22% 131 40668 0.22% 132 42109 0.23% 133 43503 0.23% 134 43662 0.23% 135 44658 0.24% 136 45713 0.25% 137 46985 0.25% 138 48288 0.26% 139 49565 0.27% 140 49934 0.27% 141 50456 0.27% 142 51424 0.28% 143 51670 0.28% 144 53681 0.29% 145 54844 0.29% 146 54449 0.29% 147 55979 0.30% 148 57848 0.31% 149 58503 0.31% 150 60059 0.32% 151 16670643 89.40% 18647808 reads passed initial QC criterion=sequence-density sequence-density=0.39 sequence-density-rank=1 fanout-score=2.01 fanout-score-rank=21 prefix-density=0.40 prefix-fanout=2.0 sequence=CGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTT criterion=fanout-score sequence-density=0.02 sequence-density-rank=27 fanout-score=286.91 fanout-score-rank=1 prefix-density=0.28 prefix-fanout=16.4 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGGGTATGAATGTGTTCTCTGTG criterion=sequence-density sequence-density=0.46 sequence-density-rank=1 fanout-score=2.16 fanout-score-rank=23 prefix-density=0.47 prefix-fanout=2.1 sequence=TGTAAGAGATGGCTTCCTC criterion=fanout-score sequence-density=0.11 sequence-density-rank=26 fanout-score=41.55 fanout-score-rank=1 prefix-density=0.32 prefix-fanout=13.6 sequence=AAAGAAAAGAAAA SRR13695439 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 02:59:04 Started mapping on | Feb 12 02:59:05 Finished on | Feb 12 03:00:59 Mapping speed, Million of reads per hour | 588.88 Number of input reads | 18647808 Average input read length | 297 UNIQUE READS: Uniquely mapped reads number | 17545484 Uniquely mapped reads % | 94.09% Average mapped length | 295.53 Number of splices: Total | 17087011 Number of splices: Annotated (sjdb) | 16713322 Number of splices: GT/AG | 16741709 Number of splices: GC/AG | 270518 Number of splices: AT/AC | 10255 Number of splices: Non-canonical | 64529 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 2.83 Insertion rate per base | 0.02% Insertion average length | 2.36 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 418831 % of reads mapped to multiple loci | 2.25% Number of reads mapped to too many loci | 33481 % of reads mapped to too many loci | 0.18% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.38% % of reads unmapped: other | 0.11% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 683733 683733 683733 N_multimapping 418831 418831 418831 N_noFeature 704864 17067824 976775 N_ambiguous 308156 1758 101257 UnstrandedReadsAssigned:16532464 PositiveStrandReadsAssigned:475902 NegativeStrandReadsAssigned:16467452 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695439 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695439-trimmed-pair1.fastq SRR13695439-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 18,647,808 reads, 16,486,438 reads pseudoaligned [quant] estimated average fragment length: 249.774 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,098 rounds 52401 SRR13695439.ke.tsv 34699 SRR13695439.se.tsv 87100 total ==> SRR13695439.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1769.23 636 17.9245 Potri.005G024800.1.v4.1 1035 786.226 401 25.4315 Potri.004G059700.1.v4.1 961 712.322 7 0.49 Potri.007G009000.2.v4.1 1416 1167.23 0 0 Potri.003G141000.2.v4.1 2943 2694.23 1157.03 21.4133 Potri.016G087400.1.v4.1 270 82.6929 873 526.405 Potri.015G069301.1.v4.1 564 323.568 0 0 Potri.010G195200.1.v4.1 1773 1524.23 164 5.36499 Potri.012G127500.1.v4.1 977 728.272 401 27.4552 ==> SRR13695439.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 429 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 282 Potri.001G212900.v4.1 9 Potri.001G182400.v4.1 3 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 6 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 8 SRR13695439 completed mapping pipeline successfully