Starting /dee2/code/volunteer_pipeline.sh SRR13695440 current disk space = 3048862240768 free memory = 1582279080 SRR13695440 SRAfilesize c3ebc4eb7dbf303b3644467caca7bd92 SRR13695440.sra SRR13695440.sra file validated SRR13695440 is paired end SRR13695440 is conventional basespace SRR13695440 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695440_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5175 37.0 37.0 37.0 37.0 37.0 2 36.2135 37.0 37.0 37.0 37.0 37.0 3 36.505 37.0 37.0 37.0 37.0 37.0 4 36.4895 37.0 37.0 37.0 37.0 37.0 5 36.5845 37.0 37.0 37.0 37.0 37.0 6 36.575 37.0 37.0 37.0 37.0 37.0 7 36.3995 37.0 37.0 37.0 37.0 37.0 8 36.5875 37.0 37.0 37.0 37.0 37.0 9 36.584 37.0 37.0 37.0 37.0 37.0 10-14 36.5862 37.0 37.0 37.0 37.0 37.0 15-19 36.560900000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.5227 37.0 37.0 37.0 37.0 37.0 25-29 36.465900000000005 37.0 37.0 37.0 37.0 37.0 30-34 36.4619 37.0 37.0 37.0 37.0 37.0 35-39 36.442 37.0 37.0 37.0 37.0 37.0 40-44 36.4364 37.0 37.0 37.0 37.0 37.0 45-49 36.40939999999999 37.0 37.0 37.0 37.0 37.0 50-54 36.3613 37.0 37.0 37.0 37.0 37.0 55-59 36.3909 37.0 37.0 37.0 37.0 37.0 60-64 36.3939 37.0 37.0 37.0 37.0 37.0 65-69 36.319 37.0 37.0 37.0 37.0 37.0 70-74 36.3052 37.0 37.0 37.0 37.0 37.0 75-79 36.2898 37.0 37.0 37.0 37.0 37.0 80-84 36.2384 37.0 37.0 37.0 37.0 37.0 85-89 36.2592 37.0 37.0 37.0 37.0 37.0 90-94 36.2015 37.0 37.0 37.0 37.0 37.0 95-99 36.1349 37.0 37.0 37.0 37.0 37.0 100-104 36.1165 37.0 37.0 37.0 37.0 37.0 105-109 36.134299999999996 37.0 37.0 37.0 37.0 37.0 110-114 36.0572 37.0 37.0 37.0 37.0 37.0 115-119 36.0865 37.0 37.0 37.0 37.0 37.0 120-124 35.974399999999996 37.0 37.0 37.0 37.0 37.0 125-129 35.9836 37.0 37.0 37.0 37.0 37.0 130-134 35.9543 37.0 37.0 37.0 37.0 37.0 135-139 35.90069999999999 37.0 37.0 37.0 37.0 37.0 140-144 35.7542 37.0 37.0 37.0 37.0 37.0 145-149 35.622499999999995 37.0 37.0 37.0 37.0 37.0 150-151 35.4825 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 0.0 22 1.0 23 0.0 24 1.0 25 2.0 26 5.0 27 7.0 28 12.0 29 11.0 30 18.0 31 35.0 32 56.0 33 67.0 34 144.0 35 356.0 36 2988.0 37 296.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.05 10.65 8.075000000000001 46.225 2 18.024132730015083 13.700351935646054 37.531422825540474 30.744092508798392 3 17.7 15.9 27.900000000000002 38.5 4 21.825 25.074999999999996 23.125 29.975 5 25.374999999999996 30.475 23.674999999999997 20.474999999999998 6 18.9 34.975 24.0 22.125 7 16.2 26.674999999999997 41.075 16.05 8 16.675 25.2 33.75 24.375 9 17.625 23.225 35.925000000000004 23.225 10-14 19.91 29.134999999999998 28.07 22.884999999999998 15-19 20.455000000000002 27.72 28.249999999999996 23.575 20-24 19.52 28.275 28.244999999999997 23.96 25-29 19.24 28.970000000000002 28.125 23.665 30-34 19.93 27.785 28.71 23.575 35-39 19.8 27.775 28.43 23.995 40-44 20.5 28.18 27.88 23.44 45-49 19.439999999999998 29.215000000000003 27.915 23.43 50-54 20.075000000000003 28.03 27.97 23.925 55-59 20.13 28.815 27.705000000000002 23.35 60-64 19.765 28.32 28.77 23.145 65-69 20.015 28.549999999999997 27.810000000000002 23.625 70-74 20.46 28.075 28.205000000000002 23.26 75-79 20.335 27.72 28.349999999999998 23.595 80-84 20.0 28.67 28.299999999999997 23.03 85-89 20.335 28.615000000000002 27.74 23.31 90-94 20.21 28.144999999999996 27.925 23.72 95-99 20.47 27.474999999999998 28.305000000000003 23.75 100-104 20.465 27.63 28.64 23.265 105-109 19.765 28.12 28.854999999999997 23.26 110-114 20.169999999999998 29.005 27.74 23.085 115-119 19.84 28.71 27.96 23.49 120-124 20.285 28.48 27.775 23.46 125-129 20.305 29.185 26.674999999999997 23.835 130-134 20.13 28.985 27.12 23.765 135-139 20.560000000000002 29.015 27.175 23.25 140-144 20.71 28.599999999999998 27.27 23.419999999999998 145-149 20.59 28.645 28.21 22.555 150-151 20.65 28.1875 27.437499999999996 23.724999999999998 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 2.0 18 2.5 19 0.5 20 0.0 21 0.5 22 1.0 23 3.5 24 4.0 25 2.0 26 2.0 27 5.0 28 10.0 29 12.5 30 10.5 31 17.0 32 31.0 33 42.5 34 67.5 35 77.5 36 72.5 37 99.0 38 133.5 39 158.0 40 190.5 41 237.5 42 261.0 43 262.0 44 272.5 45 283.5 46 284.0 47 261.5 48 231.0 49 187.5 50 157.5 51 135.0 52 104.0 53 84.0 54 74.0 55 65.5 56 50.0 57 30.5 58 18.5 59 18.0 60 11.0 61 4.5 62 3.0 63 6.0 64 4.5 65 0.5 66 0.5 67 2.5 68 2.0 69 0.0 70 0.5 71 0.5 72 0.0 73 0.0 74 0.5 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.5499999999999999 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.57499999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 69.69583176868194 46.400000000000006 2 18.13743897859557 24.15 3 7.360120165227188 14.7 4 2.891475779196395 7.7 5 1.2016522718738265 4.0 6 0.5632745024408562 2.25 7 0.11265490048817123 0.525 8 0.0 0.0 9 0.0 0.0 >10 0.03755163349605708 0.27499999999999997 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CAATGATTGTCTGACTTGTGGTGGTCTCGGAGAAACTCAAGTCTGGGTAC 11 0.27499999999999997 No Hit CCCAGTGCAGTTATGCGTGACTCAACTATTTTCTTCGCTTCAGCCAAAAC 7 0.17500000000000002 No Hit CTGACAACATCAAGAACAGAGTCAATCAATTCAGCTCCTTCAGTATAATG 7 0.17500000000000002 No Hit CTTTTCTTTCCTTCCAGTCCTATCAAGTGAGTGATAATCATCTAAACATA 7 0.17500000000000002 No Hit TCTGCTTTGAGGCTATATCATCAAAGATATTACTTTCCAGCTCCTCAGAA 6 0.15 No Hit CCCGTGATCATGGTTTGGTAATTGAGCAAAGACTCCAATCTACCCTGAGA 6 0.15 No Hit CCTTGGCTCACCACCACACATGATGGGCTGATTAAAACCTCCATTAAAAG 6 0.15 No Hit CCCCAATTCAACAACTTCATGCTTTCTTTAGTAAGTTTGCCTCCAACAGG 6 0.15 No Hit CCCGATCCCGCCTTCATAAAACGAGCCCGCGAAGTCTACAGTTTGGGCCT 6 0.15 No Hit GTCCCAACCTTTTCATATTTGCTCATGAACTTGTATTCCCAGTCCTGCAA 6 0.15 No Hit CACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGA 6 0.15 No Hit CTTCGATTTGTTTCCGAGTACTGGCTAGGAGAGATGTTTCAGTAAGATGA 6 0.15 No Hit CTCTCATCACATCACCATTATCAGAAACGTCATTACCCGACATCAATCCA 6 0.15 No Hit AGCTCTTTCTCCTTCTCCCCAGCAATCTGCAGAGCAACTTCAAGGCTTGA 6 0.15 No Hit CACCTCTTCTGATCCACGATCATTTTTTACTCGGCATGCCGCAAATCGAG 6 0.15 No Hit CGGGACTCTGTTCTCAATCTCATTATCCACTACCCCACCAGAAGAGGTCA 6 0.15 No Hit GTTCTGTCCCGTGGGTTAAGTAAGGATACATGCACTGCCAAGTCCCTCCG 6 0.15 No Hit GTGCGTAGAACAAAGCGTATACATAGAAAGTTTGTATGTGCAGCTCTAAG 6 0.15 No Hit CTCGTTGTAATCACGATTCCTTAAAATCTCTCTAATCTTATCATCAGAGC 6 0.15 No Hit ACTCTTTCTGGTTGGGGCTCGTTATAGTCAACAAACATTTTGTTTGCACG 5 0.125 No Hit GAAAGTGAGTTGGTCTTCTTTGAGACCCATTGAAATTGCAGGGTGTGCTT 5 0.125 No Hit ACCTGTGCTGCCATGGTATCCAACCTGTAGAGTTGTAGCCAGTGGTTTCC 5 0.125 No Hit CCACACTTGCAGCCATTCTCGGCTCCCACGACCGTCTCAGCAGCTCCCGC 5 0.125 No Hit ACGAGCTTGAACTGTCTGCAAATTGATCAGCGAGGATCATCCCAACACCA 5 0.125 No Hit TGGGCACTTCCGAGCTTGGTGCGAAGATGCTTGGCAGCTGCGACCATGTT 5 0.125 No Hit GTTGAGCTTAGTTCATGCCCACCCTTTCCAAGGTCATCTTCTAGCTCATG 5 0.125 No Hit ATTGGCTTTTCCCTTGCTTCTCTCTCTTCTGCTTTGACCCTGAATTCGAC 5 0.125 No Hit TTTGAGGCTCTCTTCATTTGTAGCATAATTAACTATTCTGAGAGATCAGG 5 0.125 No Hit GCCTGATATTTTTCGAAGTCGTCTGGCTTCTTGCTGAGACCAAAAGGATC 5 0.125 No Hit CTCTCTTCCCATGCTTTGACAAGTGATATCCTCTTCTCTGTTGCAACCCT 5 0.125 No Hit GTGAGGAGTGGGCTTTGCATATTGCCAGGCATGAGGCAACTTAGCCTTAG 5 0.125 No Hit AGGTCTAAGGCTTGACCCACTAGTTGATATTGAAAACTGTGGGTAAAGGG 5 0.125 No Hit GTGCAGATGAAGGTTTTTCAGAACCTGATAACCTGAGCTTCCCAAAATCA 5 0.125 No Hit CGTGCGATCTCCCCTCCAAACACGAAAATGATAATTTTATTAAGATAGTG 5 0.125 No Hit CATAAAAGTAAGATTGATTAGAAAAGATATCAGTGCTGGAGGAATTAGAC 5 0.125 No Hit CCCAAACCCACTCACCTGAGGAGCCAACTCGGTCCCTTCTCTCTCTACTT 5 0.125 No Hit GCAGGAAATGAACAGTAATTCATGAAAACATGCATGTCCATCCTACCACT 5 0.125 No Hit ATAATCTCCAGCCTCACCAAACATCTTTGCCAATACAGAGAATGTTATAG 5 0.125 No Hit CTGGTTTATCGTTTTCCAGGTCTATTTTGCTAATGAACTATCATGTTACA 5 0.125 No Hit CTCAGCAAGCCCGAGTGGGTCAAACCCATTATCACCTGGAAGGCTGCCAT 5 0.125 No Hit AATTGAGAAGCATAGAGAGCAGAGAACTCCCACTAGAAAAGATGTTTCTG 5 0.125 No Hit CCCATCGATTAATCATGAAAAAGGAAACAAATTCAACACGAAACAATCAT 5 0.125 No Hit CTCAACTGGTAACAAACTACGAACATCATGCTGCTCAAAGCGAGAGGCTA 5 0.125 No Hit TCATTCTTGAATGCCAGGAAGTCAAACACTGAATGAAGCATTGAAACGAC 5 0.125 No Hit GACGTTTAGATGGTCTCATATACTGGCCAAACGTCATCACATCAATACCT 5 0.125 No Hit CTCCTCTATAAAACCGGAAGAATGGTAACACATGGACATTGAGGCTATAA 5 0.125 No Hit GTTGTCGGTGTATCCAAACAGGGAAGACGAGAGAATCGGAGACTTTTTGA 5 0.125 No Hit GCTTTATATGAGATTTTACATCGTCTCTCTGCAAGTTTATCACTTAATGC 5 0.125 No Hit GCAGAAGCTAAGAGAGGGAGCTAGAATGTAGGCAACAACTACATAGTACC 5 0.125 No Hit GCCTTCCTTGTCTTGGATCTTGGCTTTCACATTGTCAATGGTATCAGAGC 5 0.125 No Hit CTTAACTTTATTTTACTCCCTCCTCCTCTCTCTCTCTCTCTCTCCACCCT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0125 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.05 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.21250000000000002 0.0 0.0 0.0 0.0 84-85 0.2375 0.0 0.0 0.0 0.0 86-87 0.275 0.0 0.0 0.0 0.0 88-89 0.3125 0.0 0.0 0.0 0.0 90-91 0.45 0.0 0.0 0.0 0.0 92-93 0.55 0.0 0.0 0.0 0.0 94-95 0.7375 0.0 0.0 0.0 0.0 96-97 0.9125000000000001 0.0 0.0 0.0 0.0 98-99 0.95 0.0 0.0 0.0 0.0 100-101 0.975 0.0 0.0 0.0 0.0 102-103 1.1 0.0 0.0 0.0 0.0 104-105 1.15 0.0 0.0 0.0 0.0 106-107 1.3 0.0 0.0 0.0 0.0 108-109 1.5 0.0 0.0 0.0 0.0 110-111 1.7000000000000002 0.0 0.0 0.0 0.0 112-113 1.9375 0.0 0.0 0.0 0.0 114-115 2.1375 0.0 0.0 0.0 0.0 116-117 2.325 0.0 0.0 0.0 0.0 118-119 2.55 0.0 0.0 0.0 0.0 120-121 2.8875 0.0 0.0 0.0 0.0 122-123 3.1625 0.0 0.0 0.0 0.0 124-125 3.4 0.0 0.0 0.0 0.0 126-127 3.7874999999999996 0.0 0.0 0.0 0.0 128-129 3.95 0.0 0.0 0.0 0.0 130-131 4.1375 0.0 0.0 0.0 0.0 132-133 4.55 0.0 0.0 0.0 0.0 134-135 4.9125 0.0 0.0 0.0 0.0 136-137 5.3125 0.0 0.0 0.0 0.0 138-139 5.775 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCTCC 10 0.006830828 145.0 3 TCCGCTC 10 0.006830828 145.0 2 >>END_MODULE SRR13695440 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695440_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.1505 37.0 37.0 37.0 37.0 37.0 2 36.2485 37.0 37.0 37.0 37.0 37.0 3 36.092 37.0 37.0 37.0 37.0 37.0 4 36.115 37.0 37.0 37.0 37.0 37.0 5 36.1975 37.0 37.0 37.0 37.0 37.0 6 36.2085 37.0 37.0 37.0 37.0 37.0 7 36.225 37.0 37.0 37.0 37.0 37.0 8 36.3285 37.0 37.0 37.0 37.0 37.0 9 36.232 37.0 37.0 37.0 37.0 37.0 10-14 36.2929 37.0 37.0 37.0 37.0 37.0 15-19 36.2691 37.0 37.0 37.0 37.0 37.0 20-24 36.2207 37.0 37.0 37.0 37.0 37.0 25-29 36.1688 37.0 37.0 37.0 37.0 37.0 30-34 36.12820000000001 37.0 37.0 37.0 37.0 37.0 35-39 36.11 37.0 37.0 37.0 37.0 37.0 40-44 36.15069999999999 37.0 37.0 37.0 37.0 37.0 45-49 36.148199999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.07675 37.0 37.0 37.0 37.0 37.0 55-59 36.0867 37.0 37.0 37.0 37.0 37.0 60-64 35.9623 37.0 37.0 37.0 37.0 37.0 65-69 35.985699999999994 37.0 37.0 37.0 37.0 37.0 70-74 35.9218 37.0 37.0 37.0 37.0 37.0 75-79 35.958600000000004 37.0 37.0 37.0 37.0 37.0 80-84 35.8767 37.0 37.0 37.0 37.0 37.0 85-89 35.8026 37.0 37.0 37.0 37.0 37.0 90-94 35.7906 37.0 37.0 37.0 37.0 37.0 95-99 35.835 37.0 37.0 37.0 37.0 37.0 100-104 35.7333 37.0 37.0 37.0 37.0 37.0 105-109 35.760000000000005 37.0 37.0 37.0 37.0 37.0 110-114 35.74249999999999 37.0 37.0 37.0 37.0 37.0 115-119 35.674 37.0 37.0 37.0 37.0 37.0 120-124 35.6203 37.0 37.0 37.0 37.0 37.0 125-129 35.6409 37.0 37.0 37.0 37.0 37.0 130-134 35.4451 37.0 37.0 37.0 37.0 37.0 135-139 35.5698 37.0 37.0 37.0 37.0 37.0 140-144 35.425 37.0 37.0 37.0 37.0 37.0 145-149 35.361900000000006 37.0 37.0 37.0 34.6 37.0 150-151 35.093500000000006 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 2.0 18 1.0 19 0.0 20 1.0 21 1.0 22 0.0 23 2.0 24 4.0 25 6.0 26 6.0 27 9.0 28 23.0 29 11.0 30 24.0 31 43.0 32 67.0 33 142.0 34 218.0 35 632.0 36 2621.0 37 186.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.99298597194389 19.03807615230461 13.902805611222444 33.06613226452906 2 25.974999999999998 25.624999999999996 33.025 15.375 3 19.85 29.225 31.025000000000002 19.900000000000002 4 23.45 34.525 24.15 17.875 5 25.0 34.849999999999994 23.325000000000003 16.825000000000003 6 19.0 38.425 24.15 18.425 7 18.45 20.349999999999998 40.625 20.575 8 19.85 26.35 29.425 24.375 9 22.15 24.375 31.025000000000002 22.45 10-14 22.78 29.659999999999997 26.534999999999997 21.025 15-19 22.63 27.92 28.999999999999996 20.45 20-24 21.057105710571054 28.972897289728973 28.587858785878588 21.382138213821385 25-29 21.750875437718857 28.82441220610305 28.37418709354677 21.050525262631314 30-34 22.269453890778156 27.655531106221243 29.260852170434088 20.814162832566513 35-39 22.182218221822183 28.762876287628764 28.237823782378236 20.817081708170818 40-44 22.15664699409823 27.9333800140042 29.113734120236074 20.796238871661497 45-49 22.244448889777956 28.62572514502901 28.585717143428685 20.544108821764354 50-54 22.78113905695285 28.761438071903594 28.596429821491075 19.860993049652485 55-59 22.18609304652326 28.74937468734367 27.61880940470235 21.445722861430717 60-64 22.742274227422744 28.06780678067807 28.552855285528555 20.637063706370636 65-69 22.585 27.515 28.375 21.525 70-74 22.34617308654327 29.03951975987994 27.878939469734863 20.735367683841922 75-79 22.471741522456735 28.443533059917975 28.678603581074324 20.406121836550966 80-84 22.5 27.765 28.055000000000003 21.68 85-89 23.539415766306522 27.450980392156865 28.38635454181673 20.623249299719888 90-94 22.882288228822883 27.652765276527653 28.517851785178514 20.94709470947095 95-99 23.115 28.12 28.095 20.669999999999998 100-104 23.676838419209606 27.688844422211105 28.30415207603802 20.33016508254127 105-109 23.364345738295317 28.141256502601042 27.931172468987597 20.563225290116048 110-114 22.772277227722775 28.782878287828783 28.377837783778375 20.067006700670067 115-119 22.87186155846754 28.258477543262977 27.853356006802038 21.016304891467442 120-124 23.38169084542271 28.289144572286144 27.888944472236116 20.440220110055026 125-129 23.699479791916765 28.156262505002 27.831132452981194 20.31312525010004 130-134 24.007003501750876 27.938969484742373 27.188594297148573 20.86543271635818 135-139 23.976988494247124 28.104052026013004 28.14407203601801 19.774887443721862 140-144 24.102410241024103 27.53775377537754 27.467746774677465 20.89208920892089 145-149 25.27263631815908 27.70385192596298 27.40370185092546 19.619809904952476 150-151 24.61230615307654 29.152076038019008 27.063531765882942 19.172086043021512 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.5 17 2.0 18 1.0 19 0.5 20 0.0 21 0.5 22 1.5 23 5.0 24 6.0 25 6.5 26 7.5 27 10.0 28 15.5 29 19.5 30 18.0 31 25.5 32 38.0 33 51.5 34 67.5 35 79.5 36 93.5 37 110.0 38 141.5 39 181.5 40 213.0 41 224.5 42 249.5 43 267.0 44 283.0 45 277.0 46 267.5 47 259.5 48 207.0 49 180.5 50 162.5 51 132.5 52 100.5 53 75.5 54 56.5 55 40.0 56 32.5 57 26.5 58 17.0 59 10.0 60 9.5 61 6.5 62 3.0 63 4.5 64 4.0 65 2.5 66 1.5 67 0.0 68 0.5 69 1.0 70 0.5 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.01 25-29 0.05 30-34 0.02 35-39 0.01 40-44 0.03 45-49 0.02 50-54 0.005 55-59 0.05 60-64 0.01 65-69 0.0 70-74 0.05 75-79 0.03 80-84 0.0 85-89 0.04 90-94 0.01 95-99 0.0 100-104 0.05 105-109 0.04 110-114 0.01 115-119 0.03 120-124 0.05 125-129 0.04 130-134 0.05 135-139 0.05 140-144 0.01 145-149 0.05 150-151 0.05 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.675 #Duplication Level Percentage of deduplicated Percentage of total 1 70.26621672290963 46.85 2 17.810273715785527 23.75 3 6.899137607799025 13.8 4 2.8496437945256843 7.6 5 1.4623172103487065 4.875 6 0.5249343832020997 2.1 7 0.11248593925759282 0.525 8 0.0 0.0 9 0.0 0.0 >10 0.07499062617172854 0.5 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CAGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 10 0.25 No Hit TGAGCTTGAGTGCATTCTTCTAAGTAAAAGAAATCCCTTAATTTCATCAC 10 0.25 No Hit AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 7 0.17500000000000002 No Hit CAATGTGGGAACCAAATTGGGGCCAAGTTTTGGGAGGTGATCTGTGATGA 7 0.17500000000000002 No Hit TGATAGCAGACCGATTCACGTCATCTCGTAGTGTTGAGGAATTGAAAGAT 7 0.17500000000000002 No Hit GGGAGCAGTCAAGCTGAATCTGGTTCCTGTTTGATGAATTCACATGGTTT 6 0.15 No Hit TGAGGACCGAGCAAATATGCACCACCAGCGCAGCCTTGAACTTGAAGATT 6 0.15 No Hit CTCTTTTCAAGTACTCACCTTTCTTTGCTCTCCTCCTCTTGTCTGAACCA 6 0.15 No Hit CAGGGATAAACTATCTACTAAACAACCTGAAGCTTATGAATGGTTTTGGT 6 0.15 No Hit CCCGTCTTCTGGAAAACCATGAACGGCCTTCCTCCATCTTCTGGGGAAAA 6 0.15 No Hit TGTTTCGATTTCTTCAAAAGCCCTTGGCCCAATTCATATCTGTTGCGAGG 6 0.15 No Hit GTTTGTTGATTCAAGATTCAATTGTCAAAACAGGGCCTTCAGTGTGGACA 6 0.15 No Hit GGCCAATGCTGCCAGCTTGAAAGAAGAGATCACTCGGTTGAACTTTCATG 6 0.15 No Hit CTTCTACTTCTTCTTCGCCTACATCAAATTTAGTTTCATCCCCATTTTAC 6 0.15 No Hit AAGGACTACAGCAGAGCATTGACAATTTTCATTCTGGCTTCTGTCTTTGG 6 0.15 No Hit TTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAATC 6 0.15 No Hit AGGGGCACGACATAAAATGGCTGACAAAGTAATATGTTTTTGGTAATGGT 6 0.15 No Hit GATCTATGATGTTTCTCAAAGCAGGATGTTTTATGGACCTGGCGGACCTT 6 0.15 No Hit TTTTCAGGCATGCCTAATTATTACCATTATTTGTATTGGTGTTTCATTTA 6 0.15 No Hit AGTAGGTTCAGTAAACTCATCCCAGTACTGTCATAAATCTTCGTGTGATT 5 0.125 No Hit CATCATTTGACCCTCTGTCTCTCTATAAACTGACCTTCTTAAATTAAATT 5 0.125 No Hit GTATCTTTTTTATTCTGCTTAGACTATGATTTAAAGAATCTTTTAGAATC 5 0.125 No Hit CTTCAGTTCCCATACTTTATCATGACCTCCCGGAATGACCTGTATCAGAT 5 0.125 No Hit CATGGCTGAAGATTGGTTATGTGAGCTGTGGTGTAGCATGTTATGTGTAA 5 0.125 No Hit AAGACGGGCACTCATCTTGTATCTCTCTCCGGGGGGTTTGCCATTCAAAG 5 0.125 No Hit GTGGGTTCGCCAGTCTTACAGAAACCCCCAAAGATTCAACTGCGTTGATG 5 0.125 No Hit CGGGTTGCTATGATGCTGATATATTGGCTGCCTTCGACGCTGCTATTCAA 5 0.125 No Hit CCTGATGAGGTCACATATTCTGCTATTTTAGATGTTTATGCTAAATTAGG 5 0.125 No Hit GCAAGATTCCTCAGGCGTGATAAACCAATCCCTTCTTGGTCTGATTCTGA 5 0.125 No Hit CAGATATCGCCCCATATCGCGGGCTTCTATTGGGTCTTTTTTTCATGACG 5 0.125 No Hit CCGCATTTTAATCCTAAAAAATTGACACATGGTGCTCCTGAGGATGAAAT 5 0.125 No Hit TGAACATATGAATTATCTGGCATCAAAGAATAAGGTTTTTAAGTCCTATA 5 0.125 No Hit AGATGGCCTCTACAATTTTCTCAAGATACTCTATTTCACTGCCAGGAGTA 5 0.125 No Hit CCCAACCACAACACAAAACCAGTAGAGAGAGACTCAAGGTAGGTCTCTTA 5 0.125 No Hit TCCTGATACTGTTGGCAATGGAAGTTCGATGAATGATGATCAGACCAGTG 5 0.125 No Hit CTGACACACAGACTGTAGTAATTGGTCTAGCTGCAGACTCTGGGTGTGGT 5 0.125 No Hit CGTAGACAACAAACGCGTGAATGCATACACAAGACCTTTACTAAGGCTAA 5 0.125 No Hit CACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATC 5 0.125 No Hit TGATAACTCGATCAACTCAACTCAGTTCCTCCCAACCTCAACTCGGTAGC 5 0.125 No Hit CAAATACGGGAAAAAAAAAAAAAACGATCTCTCTCTCTAACTATTTCCAT 5 0.125 No Hit CGCACCACTCCCTTTGAACTACAACCAACGGTTCTAACTTCTAACCAGGT 5 0.125 No Hit AATGGTTTAGTTGTTTTTTTATATTTTTTTGATGGATTTAGTTTTCTGGA 5 0.125 No Hit GCCATTTGAGTGGCTGGTGCAGTTGGTTCCTCCATTGAAAACAAGGGCTT 5 0.125 No Hit TCATTCTTGACTTCAGATATGCAGACTTGAATGTTGAGCCTACTTCAGGG 5 0.125 No Hit TTATCCCGGGGGCTTGCTAGCTTCTCGTCTTCTCTTATTTTATTAAGTGG 5 0.125 No Hit CAAGACAAGGAGGGTATCCCACCAGACCAGCAGAGACTCATCTTTGCCGG 5 0.125 No Hit GTCATCTCCTAGTTTCAAGGTTGAAGCCAAGAAAGGAGAGTGGTTACCTG 5 0.125 No Hit CTTGTTCATCGAGCTCAATTGACTTCTGGTCAGGTGTTGTTGGTTCTTGG 5 0.125 No Hit AGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATC 5 0.125 No Hit CCCTGACATGAGCTCCTCAGAGACGATCACCAACGAAACTCTGGTTCTTG 5 0.125 No Hit CCTAATGCTCCTCTCGGGAGTCCTGGACCTATTAGACCGCCCTACTTCTC 5 0.125 No Hit CGAAGCCTTAGTTCCTGATTTTCGAGGAGACCGTGGTTGTGTAGAGAAAG 5 0.125 No Hit ATTTAATTTGAAAAATTACATTATGCTGGCCAAATCAATATCTACCAGTA 5 0.125 No Hit GTAAATTGTTGGACTGGTTTACTTTTCTTGCTTGATTAAGGAACTATTGG 5 0.125 No Hit CTTCTGTCAGTCTTCCGTGAGGGCGTGAAATATGGTGCAGGCATTGGGCC 5 0.125 No Hit TATAGCTAACATGTCTCCTGAGTATGGTGCAACTATGGGGTTCTTCCCAG 5 0.125 No Hit GTCTGCTTCTCCGGCTGCATCAACTCCTGCCACGTTCAAGACTGTTGCTC 5 0.125 No Hit CTAAGACCAATAACATACATTTCTGCTACTTCTTCTACAGTTAAATTTCA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.05 0.0 0.0 0.0 8 0.0 0.05 0.0 0.0 0.0 9 0.0 0.05 0.0 0.0 0.0 10-11 0.0 0.05 0.0 0.0 0.0 12-13 0.0 0.05 0.0 0.0 0.0 14-15 0.0 0.05 0.0 0.0 0.0 16-17 0.0 0.05 0.0 0.0 0.0 18-19 0.0 0.05 0.0 0.0 0.0 20-21 0.0 0.05 0.0 0.0 0.0 22-23 0.0 0.05 0.0 0.0 0.0 24-25 0.0 0.05 0.0 0.0 0.0 26-27 0.0 0.05 0.0 0.0 0.0 28-29 0.0 0.05 0.0 0.0 0.0 30-31 0.0 0.05 0.0 0.0 0.0 32-33 0.0 0.05 0.0 0.0 0.0 34-35 0.0 0.05 0.0 0.0 0.0 36-37 0.0 0.05 0.0 0.0 0.0 38-39 0.0 0.05 0.0 0.0 0.0 40-41 0.0 0.05 0.0 0.0 0.0 42-43 0.0 0.05 0.0 0.0 0.0 44-45 0.0 0.05 0.0 0.0 0.0 46-47 0.0 0.05 0.0 0.0 0.0 48-49 0.0 0.05 0.0 0.0 0.0 50-51 0.0 0.05 0.0 0.0 0.0 52-53 0.0 0.05 0.0 0.0 0.0 54-55 0.0 0.05 0.0 0.0 0.0 56-57 0.0 0.05 0.0 0.0 0.0 58-59 0.0125 0.05 0.0 0.0 0.0 60-61 0.025 0.05 0.0 0.0 0.0 62-63 0.025 0.05 0.0 0.0 0.0 64-65 0.025 0.05 0.0 0.0 0.0 66-67 0.025 0.05 0.0 0.0 0.0 68-69 0.025 0.05 0.0 0.0 0.0 70-71 0.05 0.05 0.0 0.0 0.0 72-73 0.05 0.05 0.0 0.0 0.0 74-75 0.05 0.05 0.0 0.0 0.0 76-77 0.05 0.05 0.0 0.0 0.0 78-79 0.05 0.05 0.0 0.0 0.0 80-81 0.075 0.05 0.0 0.0 0.0 82-83 0.21250000000000002 0.05 0.0 0.0 0.0 84-85 0.2375 0.05 0.0 0.0 0.0 86-87 0.275 0.05 0.0 0.0 0.0 88-89 0.3125 0.05 0.0 0.0 0.0 90-91 0.45 0.05 0.0 0.0 0.0 92-93 0.55 0.05 0.0 0.0 0.0 94-95 0.7124999999999999 0.05 0.0 0.0 0.0 96-97 0.8875 0.05 0.0 0.0 0.0 98-99 0.925 0.05 0.0 0.0 0.0 100-101 0.95 0.05 0.0 0.0125 0.0 102-103 1.0875 0.05 0.0 0.025 0.0 104-105 1.15 0.05 0.0 0.025 0.0 106-107 1.2875 0.05 0.0 0.025 0.0 108-109 1.475 0.05 0.0 0.025 0.0 110-111 1.6749999999999998 0.05 0.0 0.025 0.0 112-113 1.9125 0.05 0.0 0.025 0.0 114-115 2.1125 0.05 0.0 0.025 0.0 116-117 2.3 0.05 0.0 0.025 0.0 118-119 2.5 0.05 0.0 0.025 0.0 120-121 2.8375 0.05 0.0 0.025 0.0 122-123 3.1125 0.05 0.0 0.025 0.0 124-125 3.3625 0.05 0.0 0.025 0.0 126-127 3.7625 0.05 0.0 0.025 0.0 128-129 3.925 0.05 0.0 0.025 0.0 130-131 4.1125 0.05 0.0 0.025 0.0 132-133 4.525 0.05 0.0 0.025 0.0 134-135 4.8875 0.05 0.0 0.025 0.0 136-137 5.2875 0.05 0.0 0.025 0.0 138-139 5.75 0.05 0.0 0.025 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 904385 spots for SRR13695440.sra Written 904385 spots for SRR13695440.sra Read 904385 spots for SRR13695440.sra Written 904385 spots for SRR13695440.sra Read 904385 spots for SRR13695440.sra Written 904385 spots for SRR13695440.sra Read 904385 spots for SRR13695440.sra Written 904385 spots for SRR13695440.sra Read 904385 spots for SRR13695440.sra Written 904385 spots for SRR13695440.sra Read 904385 spots for SRR13695440.sra Written 904385 spots for SRR13695440.sra Read 904385 spots for SRR13695440.sra Written 904385 spots for SRR13695440.sra Read 904385 spots for SRR13695440.sra Written 904385 spots for SRR13695440.sra Read 904385 spots for SRR13695440.sra Written 904385 spots for SRR13695440.sra Read 904385 spots for SRR13695440.sra Written 904385 spots for SRR13695440.sra Read 904385 spots for SRR13695440.sra Written 904385 spots for SRR13695440.sra Read 904385 spots for SRR13695440.sra Written 904385 spots for SRR13695440.sra Read 904385 spots for SRR13695440.sra Written 904385 spots for SRR13695440.sra Read 904387 spots for SRR13695440.sra Written 904387 spots for SRR13695440.sra Read 904385 spots for SRR13695440.sra Written 904385 spots for SRR13695440.sra Read 904385 spots for SRR13695440.sra Written 904385 spots for SRR13695440.sra Read 904385 spots for SRR13695440.sra Written 904385 spots for SRR13695440.sra Read 904385 spots for SRR13695440.sra Written 904385 spots for SRR13695440.sra Read 904385 spots for SRR13695440.sra Written 904385 spots for SRR13695440.sra Read 904385 spots for SRR13695440.sra Written 904385 spots for SRR13695440.sra SRR ids: ['SRR13695440.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_kywazptf SRR13695440.sra spots: 18087702 blocks: [[1, 904385], [904386, 1808770], [1808771, 2713155], [2713156, 3617540], [3617541, 4521925], [4521926, 5426310], [5426311, 6330695], [6330696, 7235080], [7235081, 8139465], [8139466, 9043850], [9043851, 9948235], [9948236, 10852620], [10852621, 11757005], [11757006, 12661390], [12661391, 13565775], [13565776, 14470160], [14470161, 15374545], [15374546, 16278930], [16278931, 17183315], [17183316, 18087702]] SRR13695440 file size 6125292 SRR13695440 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695440 SRR13695440_1.fastq SRR13695440_2.fastq Input file: SRR13695440_1.fastq Paired file: SRR13695440_2.fastq trimmed: SRR13695440-trimmed-pair1.fastq, SRR13695440-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 03:31:05 2025 >> started Wed Feb 12 03:31:24 2025 >> done (19.094s) 18087702 read pairs processed; of these: 111 ( 0.00%) short read pairs filtered out after trimming by size control 1645 ( 0.01%) empty read pairs filtered out after trimming by size control 18085946 (99.99%) read pairs available; of these: 1572702 ( 8.70%) trimmed read pairs available after processing 16513244 (91.30%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 3 0.00% 19 3 0.00% 20 0 0.00% 21 2 0.00% 22 1 0.00% 23 3 0.00% 24 2 0.00% 25 6 0.00% 26 5 0.00% 27 5 0.00% 28 6 0.00% 29 5 0.00% 30 3 0.00% 31 6 0.00% 32 2 0.00% 33 3 0.00% 34 9 0.00% 35 8 0.00% 36 10 0.00% 37 9 0.00% 38 16 0.00% 39 18 0.00% 40 12 0.00% 41 9 0.00% 42 23 0.00% 43 13 0.00% 44 24 0.00% 45 17 0.00% 46 20 0.00% 47 29 0.00% 48 42 0.00% 49 53 0.00% 50 41 0.00% 51 56 0.00% 52 75 0.00% 53 75 0.00% 54 69 0.00% 55 113 0.00% 56 86 0.00% 57 113 0.00% 58 139 0.00% 59 164 0.00% 60 171 0.00% 61 252 0.00% 62 236 0.00% 63 257 0.00% 64 323 0.00% 65 350 0.00% 66 400 0.00% 67 452 0.00% 68 504 0.00% 69 534 0.00% 70 725 0.00% 71 764 0.00% 72 881 0.00% 73 1063 0.01% 74 1136 0.01% 75 1165 0.01% 76 1315 0.01% 77 1491 0.01% 78 1720 0.01% 79 1907 0.01% 80 2044 0.01% 81 2403 0.01% 82 2630 0.01% 83 2896 0.02% 84 3309 0.02% 85 3521 0.02% 86 3840 0.02% 87 4160 0.02% 88 4531 0.03% 89 4910 0.03% 90 5430 0.03% 91 5850 0.03% 92 6120 0.03% 93 6725 0.04% 94 7017 0.04% 95 7827 0.04% 96 8366 0.05% 97 9025 0.05% 98 9339 0.05% 99 9758 0.05% 100 10297 0.06% 101 10843 0.06% 102 11111 0.06% 103 11936 0.07% 104 12601 0.07% 105 13234 0.07% 106 13997 0.08% 107 14550 0.08% 108 15067 0.08% 109 16169 0.09% 110 16035 0.09% 111 16947 0.09% 112 17332 0.10% 113 18025 0.10% 114 19199 0.11% 115 20387 0.11% 116 20528 0.11% 117 21457 0.12% 118 22180 0.12% 119 22797 0.13% 120 23594 0.13% 121 24501 0.14% 122 25366 0.14% 123 26258 0.15% 124 27055 0.15% 125 27786 0.15% 126 28728 0.16% 127 29476 0.16% 128 30112 0.17% 129 31160 0.17% 130 31532 0.17% 131 32269 0.18% 132 33478 0.19% 133 34315 0.19% 134 35013 0.19% 135 35217 0.19% 136 37525 0.21% 137 37674 0.21% 138 38508 0.21% 139 39393 0.22% 140 40598 0.22% 141 41625 0.23% 142 41617 0.23% 143 42524 0.24% 144 43882 0.24% 145 44590 0.25% 146 45518 0.25% 147 46289 0.26% 148 48229 0.27% 149 48412 0.27% 150 49121 0.27% 151 16513244 91.30% 18085946 reads passed initial QC criterion=sequence-density sequence-density=0.33 sequence-density-rank=1 fanout-score=1.95 fanout-score-rank=27 prefix-density=0.34 prefix-fanout=1.9 sequence=CGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTTCGAGCGAAGAAGGTAATCAATTTCCTTGGCCAATTCCTCCTCTGTGAGATCTGGAAGGTAAGAAAGAGTCTCGAACTTCTTCAATCCAGTTGGTGGCCACACCTGCATGCATTGAACTCGTCCACCATTGCTTGCAATGGAAGTAATGTCATTAGCCTTTCTGGTACTGACTGGGAAAGCTGCGGCAGACTTGAGACCATTGAATGGTGCCACCAT criterion=fanout-score sequence-density=0.07 sequence-density-rank=22 fanout-score=14.57 fanout-score-rank=1 prefix-density=0.20 prefix-fanout=4.8 sequence=ACAGCCTTCAGCGACAGGCACCTTTACTTGCTGTGCCGCTTGGCC criterion=sequence-density sequence-density=0.49 sequence-density-rank=1 fanout-score=2.21 fanout-score-rank=30 prefix-density=0.50 prefix-fanout=2.1 sequence=TGTAAGAGATGGCTTCCTC criterion=fanout-score sequence-density=0.08 sequence-density-rank=28 fanout-score=58.78 fanout-score-rank=1 prefix-density=0.34 prefix-fanout=14.5 sequence=AAGAAAACAGATTATCAAGCTTACTAGAATTATGGAAGGAATGAGTGTGGAGAACATGCACAAGATAGTGGTGGCAGTGGATGAGAGTGAGGAGAGCATGCATGCTCTTTCATGGTGTCTCAGCAACCTTATTTCTCA SRR13695440 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 03:32:06 Started mapping on | Feb 12 03:32:10 Finished on | Feb 12 03:33:57 Mapping speed, Million of reads per hour | 608.50 Number of input reads | 18085946 Average input read length | 297 UNIQUE READS: Uniquely mapped reads number | 17113226 Uniquely mapped reads % | 94.62% Average mapped length | 296.50 Number of splices: Total | 17011272 Number of splices: Annotated (sjdb) | 16622822 Number of splices: GT/AG | 16671949 Number of splices: GC/AG | 267422 Number of splices: AT/AC | 9007 Number of splices: Non-canonical | 62894 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.03% Deletion average length | 2.99 Insertion rate per base | 0.02% Insertion average length | 2.40 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 395977 % of reads mapped to multiple loci | 2.19% Number of reads mapped to too many loci | 86349 % of reads mapped to too many loci | 0.48% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.60% % of reads unmapped: other | 0.11% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 576950 576950 576950 N_multimapping 395977 395977 395977 N_noFeature 774923 16739049 1002697 N_ambiguous 252635 1686 105103 UnstrandedReadsAssigned:16085668 PositiveStrandReadsAssigned:372491 NegativeStrandReadsAssigned:16005426 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695440 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695440-trimmed-pair1.fastq SRR13695440-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 18,085,946 reads, 16,003,478 reads pseudoaligned [quant] estimated average fragment length: 262.963 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,090 rounds 52401 SRR13695440.ke.tsv 34699 SRR13695440.se.tsv 87100 total ==> SRR13695440.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1756.04 563 18.7458 Potri.005G024800.1.v4.1 1035 773.037 447 33.8094 Potri.004G059700.1.v4.1 961 699.244 11 0.919803 Potri.007G009000.2.v4.1 1416 1154.04 0 0 Potri.003G141000.2.v4.1 2943 2681.04 1081.52 23.5865 Potri.016G087400.1.v4.1 270 79.6158 819 601.471 Potri.015G069301.1.v4.1 564 313.938 0 0 Potri.010G195200.1.v4.1 1773 1511.04 179 6.92641 Potri.012G127500.1.v4.1 977 715.166 171 13.9804 ==> SRR13695440.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 178 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 266 Potri.001G212900.v4.1 1 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 1 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 5 SRR13695440 completed mapping pipeline successfully