Starting /dee2/code/volunteer_pipeline.sh SRR13695441 current disk space = 3049004150784 free memory = 1367645544 SRR13695441 SRAfilesize 77e390a80d7c0ed0359fd0f65ee3abc5 SRR13695441.sra SRR13695441.sra file validated SRR13695441 is paired end SRR13695441 is conventional basespace SRR13695441 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695441_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5465 37.0 37.0 37.0 37.0 37.0 2 36.28125 37.0 37.0 37.0 37.0 37.0 3 36.606 37.0 37.0 37.0 37.0 37.0 4 36.5745 37.0 37.0 37.0 37.0 37.0 5 36.5445 37.0 37.0 37.0 37.0 37.0 6 36.614 37.0 37.0 37.0 37.0 37.0 7 36.5665 37.0 37.0 37.0 37.0 37.0 8 36.576 37.0 37.0 37.0 37.0 37.0 9 36.5165 37.0 37.0 37.0 37.0 37.0 10-14 36.611000000000004 37.0 37.0 37.0 37.0 37.0 15-19 36.6128 37.0 37.0 37.0 37.0 37.0 20-24 36.53789999999999 37.0 37.0 37.0 37.0 37.0 25-29 36.4816 37.0 37.0 37.0 37.0 37.0 30-34 36.4778 37.0 37.0 37.0 37.0 37.0 35-39 36.4749 37.0 37.0 37.0 37.0 37.0 40-44 36.45440000000001 37.0 37.0 37.0 37.0 37.0 45-49 36.408500000000004 37.0 37.0 37.0 37.0 37.0 50-54 36.3856 37.0 37.0 37.0 37.0 37.0 55-59 36.3914 37.0 37.0 37.0 37.0 37.0 60-64 36.3981 37.0 37.0 37.0 37.0 37.0 65-69 36.3113 37.0 37.0 37.0 37.0 37.0 70-74 36.3202 37.0 37.0 37.0 37.0 37.0 75-79 36.3227 37.0 37.0 37.0 37.0 37.0 80-84 36.2457 37.0 37.0 37.0 37.0 37.0 85-89 36.2892 37.0 37.0 37.0 37.0 37.0 90-94 36.1811 37.0 37.0 37.0 37.0 37.0 95-99 36.13080000000001 37.0 37.0 37.0 37.0 37.0 100-104 36.095099999999995 37.0 37.0 37.0 37.0 37.0 105-109 36.140100000000004 37.0 37.0 37.0 37.0 37.0 110-114 36.1052 37.0 37.0 37.0 37.0 37.0 115-119 36.0591 37.0 37.0 37.0 37.0 37.0 120-124 35.9627 37.0 37.0 37.0 37.0 37.0 125-129 35.9671 37.0 37.0 37.0 37.0 37.0 130-134 35.972699999999996 37.0 37.0 37.0 37.0 37.0 135-139 35.917500000000004 37.0 37.0 37.0 37.0 37.0 140-144 35.8131 37.0 37.0 37.0 37.0 37.0 145-149 35.6846 37.0 37.0 37.0 37.0 37.0 150-151 35.48350000000001 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 1.0 25 2.0 26 6.0 27 4.0 28 15.0 29 14.0 30 29.0 31 38.0 32 43.0 33 83.0 34 119.0 35 329.0 36 2923.0 37 393.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.075 11.5 9.475 41.949999999999996 2 20.23121387283237 13.99849208343805 38.376476501633576 27.393817542096006 3 19.45 18.6 25.05 36.9 4 20.75 26.950000000000003 23.175 29.125 5 22.75 33.875 22.5 20.875 6 19.650000000000002 34.150000000000006 23.9 22.3 7 14.95 27.1 41.575 16.375 8 18.625 26.450000000000003 28.849999999999998 26.075 9 16.825000000000003 24.349999999999998 34.575 24.25 10-14 19.09 30.44 27.495000000000005 22.975 15-19 19.900000000000002 29.845 26.450000000000003 23.805 20-24 19.99 29.395 27.029999999999998 23.585 25-29 20.315 28.749999999999996 27.615000000000002 23.32 30-34 19.545 28.95 27.93 23.575 35-39 20.54 28.595 27.49 23.375 40-44 19.425 29.065 28.035 23.474999999999998 45-49 20.1 29.020000000000003 27.045 23.835 50-54 20.05 28.63 26.88 24.44 55-59 20.365 29.020000000000003 27.815 22.8 60-64 20.330000000000002 28.71 27.215 23.745 65-69 19.56 28.555000000000003 28.15 23.735 70-74 20.294999999999998 29.220000000000002 27.310000000000002 23.175 75-79 19.405 28.249999999999996 28.555000000000003 23.79 80-84 20.549999999999997 28.125 27.62 23.705000000000002 85-89 20.13 29.255 27.705000000000002 22.91 90-94 20.095 28.754999999999995 27.92 23.23 95-99 20.315 27.87 27.67 24.145 100-104 20.27 28.860000000000003 28.075 22.795 105-109 21.235 27.284999999999997 27.42 24.060000000000002 110-114 20.265 28.825 27.74 23.169999999999998 115-119 20.560000000000002 28.43 27.395000000000003 23.615 120-124 20.330000000000002 28.525 27.83 23.315 125-129 20.46 28.134999999999998 27.3 24.104999999999997 130-134 20.44 28.660000000000004 27.425 23.474999999999998 135-139 21.165 28.29 26.865 23.68 140-144 20.87 28.33 26.974999999999998 23.825 145-149 20.235 27.68 27.474999999999998 24.610000000000003 150-151 21.224999999999998 27.212500000000002 27.975 23.5875 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 3.0 20 4.0 21 1.5 22 2.5 23 2.5 24 2.5 25 2.0 26 3.5 27 6.5 28 13.0 29 19.5 30 31.0 31 34.0 32 26.0 33 48.5 34 65.0 35 65.5 36 101.0 37 115.0 38 138.0 39 187.5 40 181.0 41 185.5 42 257.0 43 284.0 44 239.0 45 236.5 46 256.5 47 235.0 48 222.5 49 207.0 50 159.0 51 126.0 52 115.0 53 93.5 54 69.5 55 62.0 56 45.5 57 42.0 58 32.5 59 17.0 60 14.5 61 11.0 62 11.0 63 5.5 64 2.0 65 3.0 66 3.0 67 1.5 68 2.5 69 2.5 70 0.5 71 0.0 72 0.0 73 2.0 74 2.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.525 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.175 #Duplication Level Percentage of deduplicated Percentage of total 1 68.94597657725727 45.625 2 19.15375897242161 25.35 3 7.442387608613525 14.774999999999999 4 2.4933887419720437 6.6000000000000005 5 1.0200226671703816 3.375 6 0.4911220249338874 1.95 7 0.26445032111824707 1.225 8 0.07555723460521345 0.4 9 0.07555723460521345 0.44999999999999996 >10 0.037778617302606725 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTGCATTAATTCAAACGATAATGTCTTTATTAAAAAATGGTAATGCGGTC 10 0.25 No Hit GCCTCCTTCACTGCCAAGGCAGATGGGCCGTTGGGAATGCTCACAACAGT 9 0.22499999999999998 No Hit ATCCCTTCGCTTGGATACAAAAAGTCAAGCTCCACAACCTGATCAGAATA 9 0.22499999999999998 No Hit CTGCGCTTTAGTGTGATTGCACGTGTTGTTCCTATTGTTCAACTCAGTTG 8 0.2 No Hit CTAGCCAAAAACTACAAACCATGGATATTACCTATCTACAATAATGCAGT 8 0.2 No Hit CCCAGCAACAGCCTTTGCTAGAAGAGTCTTACCTGTCCCAGGAAGGCCCA 7 0.17500000000000002 No Hit GTGACAGCAACATCATAAGGCAGGAAAATGCAGGATGAACATGTATCGTT 7 0.17500000000000002 No Hit CTCGCCAACAAGGATGATAGCCTGATTTTTCTTCAAAACTTGTAAGAAAT 7 0.17500000000000002 No Hit GGAAGTGGAAAAACTACCCTGATAAGTGCTTTGTTTCGGTTAGTAGAGCC 7 0.17500000000000002 No Hit TTTCGATATTGAAAGCGTAGAAAGTGACATGCGCTGCTGTGATTAGAATC 7 0.17500000000000002 No Hit CGCACACAGTTCTACGCAAAGCACGAAAGCATAATGTAGAAGGAAGAGGG 7 0.17500000000000002 No Hit TAGCACTAAATAAAAGACATTCAGACACAAAATTTAAAGTTCTGGCTCGC 7 0.17500000000000002 No Hit CCAGCTTCAGTACATATTGCCTTTATATCTGCTCCCGAGAACTCATCCTT 6 0.15 No Hit CTCCTCTTCAACCTGCCCTTTAAATAGTGTTGTAAGAGGCCTTCCACCAA 6 0.15 No Hit CTCCATCCAAGGTATTCAGCATTGTTTGATAAGTTGGAAGAGCTTCTTCT 6 0.15 No Hit CTTAGATAAAACATGAAAGGAAAGGGAAAAAACAATTTACGGGTCAACTT 6 0.15 No Hit TCCTCATCCATGACCTTCACAAAGGGAGCCAGACAATTTGTGGTGCAAGA 6 0.15 No Hit GCAAGAAGCAAAGTATCTTATTGATTCCACATGGTATATATAATGATCGA 6 0.15 No Hit GTGGAAGGGTACCGTTCAACCCCGTGTTGTACGATAGATCCAGAACCTCC 6 0.15 No Hit GCTTCCCTTCTCGTGGCATCGTGAAGCAGATCAACATCCACACCCTCTGG 6 0.15 No Hit ACGGAGTTTTGAAATCTCAGATTGAAAGTGAGCAGACTGCATTGTAACCA 6 0.15 No Hit CTCCTTTGAGACAGGCTTCCTCTTCCTGTCGAGCTTCTCCTTCTTAGACT 6 0.15 No Hit CGTATCTTTTGGATTCCTACCCAGCGTGCATAGTCCACCCACATCAATGA 6 0.15 No Hit CATATCTCTCCACCGATTCCGGATGACTCCGCCGAGTCAACTCGGACCAA 6 0.15 No Hit TATATAAAGCAGACAAAAATTCATAAACTTGCCAATGCGCATTGCCCTAT 6 0.15 No Hit AGAGAAAGAGAGCAAAACGAGGAGAAACGATGATGATGAAGACAAGGATA 5 0.125 No Hit GTTGGTTTTGCCAGCATAGGTCCAACTGGAACACCAGGTGTGAAGCTGCA 5 0.125 No Hit CACTCATCTTGGGGTGGGCTTACTACTTAGATGCTTTCAGCAGTTATCCG 5 0.125 No Hit GTACTGACTGGGAAAGCTGCGGCAGACTTGAGACCATTGAATGGTGCCAC 5 0.125 No Hit AGGCTTTGCAGCAGCGGTTTTAGCCTTAGCTGGAGCAGCCTTTGGTTTTG 5 0.125 No Hit AGCAATTTTCAGTCTTGTAATCCAATCCAGGGACTGCAAACCATCGTCTT 5 0.125 No Hit GCCCACCAGACAAAAACATGATTCCAGGGACGGATGGGGGGATTCTCCTC 5 0.125 No Hit CTCAACAAAATCCACTGGCGGCATTTCCACTCTGTCAAACTCCCCACCAC 5 0.125 No Hit GTTGATCTTAATTAGAAGCAAAAGTAGTGAGAATGGCAATAAAGCAACAG 5 0.125 No Hit GCAAAGGAAAAGAACCTCAGTAGTAGGCTCTGGTTCTGGCTCTGGTGCTG 5 0.125 No Hit GTAGTTATATTTATGGTAATAATTAATGGATCTGTTTTTGATGCATCGTG 5 0.125 No Hit TGTGGATCTTTTTCTGCAGCTGGAGTAATCCATAAAGTAGGGCCTCAGCA 5 0.125 No Hit GGCCACACCTGCATGCATTGAACTCGTCCACCATTGCTTGCAATGGAAGT 5 0.125 No Hit CACGAACCTATCTATCTACTACCTAAACAACTTGGCTTCTGCTGATCAAA 5 0.125 No Hit CCTCCTGCTATCTCATCATGAACATATGACACCACGTACCCATCATCTTC 5 0.125 No Hit GGAAAACATAAAATGCCCTTGCCAAGCTGCAACGTAAAGATCGTTGACAA 5 0.125 No Hit CAGCAAATCAAGGATGTGACATAAAATGACCAAAAGACCATTTCAAGATT 5 0.125 No Hit CTTGATTCTTAACGGGACTGACTATGCCTTCTTCCCTCCAGTCTTTCGTT 5 0.125 No Hit ATCCTTTCTCAGTCCCAAGATTTCTTGCCCTCATCTTCTTCTTTCCACAT 5 0.125 No Hit GCAAGAAGAGCAGCAGAGACATTGAGATTTGGGACTAGACCAGTGGTGAG 5 0.125 No Hit CCATAATCCCAAGCGGAATCCATGGATCTGGCCTCGTGCAGGCTGCCTGG 5 0.125 No Hit CTCACTAACTTCAGATATCTGAGAAAGAGACTCTTGCCTAGTAAAGCTCA 5 0.125 No Hit GTTTAACGATACCCCTTCAAAGGGTCAGCTGCATTCCCAGATCTTGCATC 5 0.125 No Hit GGCTGGACAAATATCCTCTAAACTTCTTCCCAAGCACTCAATGATATCAG 5 0.125 No Hit GCAGAAGGGGCGGTGGAGCGGCACCGGACAATGGAGGCAGAAGGCAAGCG 5 0.125 No Hit TCCTCTTCATCGTCTTCGTCGGTCTCTACTTCAAAATCAACTCCATATCT 5 0.125 No Hit ATTTGGAGTTATGAAGGTGTGGCTGGGGCGCATATTGTGTTTTCTGGCTT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.0625 0.0 0.0 0.0 0.0 80-81 0.1 0.0 0.0 0.0 0.0 82-83 0.15 0.0 0.0 0.0125 0.0 84-85 0.225 0.0 0.0 0.025 0.0 86-87 0.35 0.0 0.0 0.025 0.0 88-89 0.48750000000000004 0.0 0.0 0.025 0.0 90-91 0.6125 0.0 0.0 0.025 0.0 92-93 0.75 0.0 0.0 0.025 0.0 94-95 0.8625 0.0 0.0 0.025 0.0 96-97 1.025 0.0 0.0 0.025 0.0 98-99 1.1 0.0 0.0 0.025 0.0 100-101 1.275 0.0 0.0 0.025 0.0 102-103 1.55 0.0 0.0 0.025 0.0 104-105 1.65 0.0 0.0 0.025 0.0 106-107 1.825 0.0 0.0 0.025 0.0 108-109 2.0 0.0 0.0 0.025 0.0 110-111 2.1875 0.0 0.0 0.025 0.0 112-113 2.4625 0.0 0.0 0.025 0.0 114-115 2.75 0.0 0.0 0.025 0.0 116-117 3.1375 0.0 0.0 0.025 0.0 118-119 3.575 0.0 0.0 0.025 0.0 120-121 3.95 0.0 0.0 0.025 0.0 122-123 4.4 0.0 0.0 0.025 0.0 124-125 4.9125 0.0 0.0 0.025 0.0 126-127 5.2625 0.0 0.0 0.025 0.0 128-129 5.949999999999999 0.0 0.0 0.025 0.0 130-131 6.4625 0.0 0.0 0.025 0.0 132-133 6.975 0.0 0.0 0.025 0.0 134-135 7.35 0.0 0.0 0.025 0.0 136-137 7.875 0.0 0.0 0.025 0.0 138-139 8.662500000000001 0.0 0.0 0.025 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAATCC 10 0.006830828 145.0 3 >>END_MODULE SRR13695441 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695441_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.0925 37.0 37.0 37.0 37.0 37.0 2 36.0285 37.0 37.0 37.0 37.0 37.0 3 36.083 37.0 37.0 37.0 37.0 37.0 4 36.11 37.0 37.0 37.0 37.0 37.0 5 36.1925 37.0 37.0 37.0 37.0 37.0 6 36.1605 37.0 37.0 37.0 37.0 37.0 7 36.0685 37.0 37.0 37.0 37.0 37.0 8 36.1725 37.0 37.0 37.0 37.0 37.0 9 36.2155 37.0 37.0 37.0 37.0 37.0 10-14 36.147400000000005 37.0 37.0 37.0 37.0 37.0 15-19 36.096500000000006 37.0 37.0 37.0 37.0 37.0 20-24 36.09485 37.0 37.0 37.0 37.0 37.0 25-29 35.91655 37.0 37.0 37.0 37.0 37.0 30-34 36.00975 37.0 37.0 37.0 37.0 37.0 35-39 35.95175 37.0 37.0 37.0 37.0 37.0 40-44 35.935649999999995 37.0 37.0 37.0 37.0 37.0 45-49 35.9366 37.0 37.0 37.0 37.0 37.0 50-54 35.861850000000004 37.0 37.0 37.0 37.0 37.0 55-59 35.865750000000006 37.0 37.0 37.0 37.0 37.0 60-64 35.831450000000004 37.0 37.0 37.0 37.0 37.0 65-69 35.8329 37.0 37.0 37.0 37.0 37.0 70-74 35.734750000000005 37.0 37.0 37.0 37.0 37.0 75-79 35.725350000000006 37.0 37.0 37.0 37.0 37.0 80-84 35.827299999999994 37.0 37.0 37.0 37.0 37.0 85-89 35.6514 37.0 37.0 37.0 37.0 37.0 90-94 35.613749999999996 37.0 37.0 37.0 37.0 37.0 95-99 35.637899999999995 37.0 37.0 37.0 37.0 37.0 100-104 35.62435 37.0 37.0 37.0 37.0 37.0 105-109 35.60105 37.0 37.0 37.0 37.0 37.0 110-114 35.506150000000005 37.0 37.0 37.0 37.0 37.0 115-119 35.49835 37.0 37.0 37.0 37.0 37.0 120-124 35.502449999999996 37.0 37.0 37.0 37.0 37.0 125-129 35.424 37.0 37.0 37.0 37.0 37.0 130-134 35.3396 37.0 37.0 37.0 34.6 37.0 135-139 35.30505 37.0 37.0 37.0 34.6 37.0 140-144 35.19155 37.0 37.0 37.0 27.4 37.0 145-149 34.96045 37.0 37.0 37.0 25.0 37.0 150-151 34.734375 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 2.0 14 2.0 15 3.0 16 3.0 17 2.0 18 0.0 19 1.0 20 2.0 21 0.0 22 5.0 23 7.0 24 7.0 25 10.0 26 13.0 27 11.0 28 16.0 29 14.0 30 30.0 31 49.0 32 77.0 33 131.0 34 232.0 35 667.0 36 2538.0 37 176.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.008028098344205 19.41796287004516 14.57601605619669 27.99799297541395 2 27.325 24.075 32.5 16.1 3 22.075 27.0 31.35 19.575 4 24.625 32.05 22.95 20.375 5 24.55 37.75 20.925 16.775000000000002 6 17.875 41.375 22.725 18.025 7 21.224999999999998 21.55 38.25 18.975 8 21.6 23.674999999999997 30.125 24.6 9 22.325 24.625 30.3 22.75 10-14 23.599999999999998 29.904999999999998 25.71 20.785 15-19 23.145 28.035 28.475 20.345 20-24 23.691184559227963 28.21141057052853 28.371418570928547 19.725986299314965 25-29 22.390597649412353 27.696924231057764 29.227306826706677 20.685171292823206 30-34 23.123468520278042 28.179226884032605 27.924188628294246 20.77311596739511 35-39 23.021151057552878 27.701385069253465 28.88144407220361 20.39601980099005 40-44 22.673401010151522 27.804170625593837 28.57428614292144 20.9481422213332 45-49 23.08230823082308 27.807780778077806 28.297829782978294 20.812081208120812 50-54 22.62113105655283 28.601430071503575 27.806390319515977 20.97104855242762 55-59 23.470867716929234 27.516879219804952 27.956989247311824 21.05526381595399 60-64 23.316165808290414 27.74638731936597 28.22641132056603 20.71103555177759 65-69 23.135 28.1 27.98 20.785 70-74 23.31082770692673 29.382345586396596 27.031757939484873 20.275068767191797 75-79 22.633395009251387 28.09921488223234 28.289243386507977 20.9781467220083 80-84 22.81 28.485 28.42 20.285 85-89 22.534506901380276 28.800760152030406 27.945589117823566 20.719143828765755 90-94 23.226161308065404 28.366418320916047 27.931396569828493 20.47602380119006 95-99 23.23 28.875 27.51 20.385 100-104 23.465866466616657 27.776944236059016 27.851962990747687 20.905226306576644 105-109 24.751187796949235 27.486871717929485 28.30207551887972 19.45986496624156 110-114 23.27116355817791 27.951397569878495 28.286414320716034 20.49102455122756 115-119 23.16079019754939 28.67716929232308 28.067016754188543 20.095023755938985 120-124 25.046261565391347 28.802200550137535 26.93173293323331 19.21980495123781 125-129 25.160032006401277 27.925585117023406 27.190438087617526 19.72394478895779 130-134 24.782434730419126 28.008402520756228 27.418225467640294 19.790937281184355 135-139 25.896474118529632 27.936984246061513 26.771692923230805 19.394848712178046 140-144 25.6262813140657 27.556377818890944 26.79133956697835 20.026001300065 145-149 26.376594148537137 27.35183795948987 26.786696674168542 19.48487121780445 150-151 26.81005377016381 26.997624109040892 27.147680380142553 19.044641740652747 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.5 13 1.0 14 0.5 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 2.0 22 3.0 23 2.0 24 2.5 25 3.5 26 10.0 27 13.0 28 8.0 29 6.5 30 17.0 31 27.5 32 36.0 33 53.0 34 62.5 35 86.0 36 103.0 37 102.5 38 139.5 39 173.0 40 192.0 41 231.5 42 256.0 43 258.5 44 259.5 45 262.0 46 259.5 47 241.0 48 237.5 49 225.0 50 174.5 51 134.0 52 101.0 53 76.5 54 58.5 55 33.5 56 27.0 57 28.5 58 22.5 59 18.5 60 12.0 61 7.0 62 6.0 63 2.5 64 0.0 65 1.0 66 1.0 67 0.0 68 0.0 69 0.5 70 1.0 71 2.5 72 2.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.5 78 0.5 79 0.0 80 0.5 81 0.5 82 0.5 83 0.5 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.5 91 1.0 92 0.5 93 0.5 94 1.0 95 1.0 96 1.0 97 1.0 98 1.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35000000000000003 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.005 25-29 0.025 30-34 0.015 35-39 0.005 40-44 0.015 45-49 0.01 50-54 0.005 55-59 0.025 60-64 0.005 65-69 0.0 70-74 0.025 75-79 0.015 80-84 0.0 85-89 0.02 90-94 0.005 95-99 0.0 100-104 0.025 105-109 0.025 110-114 0.005 115-119 0.025 120-124 0.025 125-129 0.02 130-134 0.03 135-139 0.025 140-144 0.005 145-149 0.025 150-151 0.0375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.225 #Duplication Level Percentage of deduplicated Percentage of total 1 70.02603198214949 47.075 2 18.74302714763853 25.2 3 6.917069542580885 13.950000000000001 4 2.4172554853105246 6.5 5 1.1156563778356265 3.75 6 0.4090740052063964 1.6500000000000001 7 0.2231312755671253 1.05 8 0.11156563778356265 0.6 9 0.03718854592785422 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCAGATTATGAGCACTGCAAGTTTTGCTTATGTAACTCTAGCGTATTGTT 9 0.22499999999999998 No Hit CAAGAGATTAAAGTTGACAAAGAGACCATTGACATGCTTGCTGCCCTTGG 8 0.2 No Hit GAATATTCCTATAGAAGGCATGGTTTCACTGCCAGCAAGTAGAGATGGAG 8 0.2 No Hit TACTAAAGAAAGACAACACTTATGTCTATATGTGTGGGTTGAAGGGAATG 8 0.2 No Hit GGAAGAGGAAGGTGAGTTTATTCGACGTGGTTGACGAGACGTCGCTTTCT 7 0.17500000000000002 No Hit GTTCAATAGCTGGTGCAAGTAATGGAAGTTCGTTGGTTGTTATGTCCAAA 7 0.17500000000000002 No Hit TTCAAGAGGAGATGTCAAAGTGATTCTGGCGACCAACCGCATTGAAACCC 7 0.17500000000000002 No Hit CTGAAAAGAGTTGGCTCTTGAGGAATGATGCTGAGCTTCATTCTTAGATC 7 0.17500000000000002 No Hit CCGTAAGTCTGGGGGTTCTGGAGGTTCAAAAGTGTCTGAGCAAGGTGAAA 7 0.17500000000000002 No Hit CGTTTCAATTAGGGTTTTGAAGGAAATGTCACAGACAGGGAAATTGATGC 7 0.17500000000000002 No Hit GTTAAAGTCAAAGGATCCTTCAAGCTTCCATCGGCTAAATCATCCGCTCC 6 0.15 No Hit GCATGTCCCAACAACCACAGGCGATGATCTTGAGACTCCAAGACAGTGGT 6 0.15 No Hit GTTATCATCACTGCTCCAGCCAAAGGCGCCGATATCCCAACCTATGTTGT 6 0.15 No Hit CGGTGAATGGAACTCCTTACCACAAAAGCTGCTTCAAATGCATCCACGGA 6 0.15 No Hit GAGAAATGTGTCCTAAAGTTCTCACGTTTTTGCTGGTTGCTTCCTTTCAG 6 0.15 No Hit CTTTGAAGAAGAACCCATTGAAGAACTTGAATGTGATGTTGAAATTGAAC 6 0.15 No Hit GGAGGTTGAGAGTGTCAAGAAGCCTTTTATGCCCCCTAGGGAGGTGCATG 6 0.15 No Hit GCATGAAGATGTGCATCTTTAGGTGTTGACATATACACAAACACACCCAA 6 0.15 No Hit ACCTAAGGTACCAGATCCTTGCTGGAGTTATTGAACAGCGACTTTTGGAG 6 0.15 No Hit AGCAAACGCAAAAGGTGATAACAATGGCGATCGATTTGTGTAAATTCCGA 6 0.15 No Hit CTCCTCTCTCGCATATTCGTTGCAAATCCAATCCGGAGGATCACAATTAA 6 0.15 No Hit CTGTACGTTCATCACAAAAGATTTCTAGATCCCAATACACCCAATGCCAG 5 0.125 No Hit GTCGAAGCTCTATCGTCCATGCATACTCCATATCTTCTCTCTATCTCCTA 5 0.125 No Hit AAAGCACCCAAAAGTGGTGGCAGTAGCGGCAGTTTGCAGAGGTCGTACGG 5 0.125 No Hit GGATTGGACCCGAAAGAGAAAGTTATGTTTTTGATGATGGATGTTAGTGA 5 0.125 No Hit CGTTTCCAGAGCCATGCTGTGCTGGCGCTGCAAGAGGCAGCTGAGGCATA 5 0.125 No Hit ATTGTTTAAAGCTTCAAAGGGGAACTGGTTAGACATACATTTCAAGGACG 5 0.125 No Hit AGAGAATTACCATTCCAGAAATAAAAAATCATCCATGGTTCTTAAAGAAC 5 0.125 No Hit CAGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 5 0.125 No Hit TTCTCCTCTCTGAACCCTGATGCTCCCATAATTGATCCTCTTGATTCCAT 5 0.125 No Hit GTGAGCATTCCCAACGGCCCATCTGCCTTGGCAGTGAAGGAGGCTGCCTG 5 0.125 No Hit AAAAGGGTCGGGCATCCATCCCTGTTCAAATTCAGCACGAGAGGTCTCGA 5 0.125 No Hit GGCGTTGTGGGGGCCAGGGTGATATCCTCTCTGGAAGTGTTGCTGTGTTC 5 0.125 No Hit ATTGTTCCCGGTATCAAAGTTGACAAGGGTTTGGTGCCTCTAGCTGGTTC 5 0.125 No Hit ATTGCTGCAAACAAAAACACTGTTCCTGGTGATGTGTCTGCCATGGTTCC 5 0.125 No Hit GCAATGGGTTCGGGGAAGTTTTGTTCAAAAGTTCTTGCATCTAAGAGAAT 5 0.125 No Hit CCCACCCGTCGAGCAGGGACGAAAGTCGGCCTTAGTGATCCGACGGTGCC 5 0.125 No Hit ATTTAGGGAAAAAGAACAGATTCGTGTACGCAGCAATTGGTGACCCTATG 5 0.125 No Hit GTCTCATCGATCTTGTTCCTGCTATGCTGCGTTGCAGCGGGGTCAAGTTT 5 0.125 No Hit AAGCAATTAAAGGAAAGAAGAATGGAAAGGCTGTAATTGTTGGAGGAGGA 5 0.125 No Hit CTCTACATGGGTTTGAATTTGCGGCCTAAGGAACCTCGCTGCAGGTGGGT 5 0.125 No Hit CCCCACCTTCCATACAAGAAAGTAGCATTAAGAAAACCTCCAAAAACACC 5 0.125 No Hit GCCAGGCGCTTCATGATTTATGTTCATGCAAAGATGATGATTGTTGATGA 5 0.125 No Hit CACAAACCATGGCAATGATCACCAGAAACACCGCCACACGCCTCCCACAC 5 0.125 No Hit CTGCGAGCCTCTGTATCTAATTCGTCTGCTTCAGACAAAGTTGAGAATTG 5 0.125 No Hit CTCTGTCTCACTGCTTCTGTGTTTCCAAATCTATCTTTCCTTGTTTAGCT 5 0.125 No Hit TTCAATTTCAGGCTTGTCTTTCTCATTGGTCTCTTCCCATTCGGTTTGAT 5 0.125 No Hit CCGGCCGGATCTAGGGTACCTTCTAGAAGCTTCTGATGATGAGCTCGGCT 5 0.125 No Hit TTTCTTGATGTGTGTTTAGCTTCTATAAATCATTCTATTGGATTCAAGAT 5 0.125 No Hit GGAATTCCTATCGTTGTCAAAAGGATCAATTTGCATAGCTTGAAACAGGA 5 0.125 No Hit GATGGCTTCCTCCTCTATGATCTCATCGGCAGCCGTTGCCACCGTCAACC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.0625 0.0 0.0 0.0 0.0 80-81 0.1 0.0 0.0 0.0 0.0 82-83 0.15 0.0 0.0 0.0 0.0 84-85 0.225 0.0 0.0 0.0 0.0 86-87 0.35 0.0 0.0 0.0 0.0 88-89 0.48750000000000004 0.0 0.0 0.0 0.0 90-91 0.6125 0.0 0.0 0.0 0.0 92-93 0.75 0.0 0.0 0.0 0.0 94-95 0.875 0.0 0.0 0.0 0.0 96-97 1.0499999999999998 0.0 0.0 0.0 0.0 98-99 1.125 0.0 0.0 0.0 0.0 100-101 1.3 0.0 0.0 0.0 0.0 102-103 1.575 0.0 0.0 0.0 0.0 104-105 1.6749999999999998 0.0 0.0 0.0 0.0 106-107 1.85 0.0 0.0 0.0 0.0 108-109 2.025 0.0 0.0 0.0 0.0 110-111 2.2375 0.0 0.0 0.0 0.0 112-113 2.475 0.0 0.0 0.0 0.0 114-115 2.75 0.0 0.0 0.0 0.0 116-117 3.125 0.0 0.0 0.0 0.0 118-119 3.55 0.0 0.0 0.0 0.0 120-121 3.925 0.0 0.0 0.0 0.0 122-123 4.35 0.0 0.0 0.0 0.0 124-125 4.862500000000001 0.0 0.0 0.0 0.0 126-127 5.2125 0.0 0.0 0.0 0.0 128-129 5.9375 0.0 0.0 0.0 0.0 130-131 6.475 0.0 0.0 0.0 0.0 132-133 7.0125 0.0 0.0 0.0 0.0 134-135 7.4 0.0 0.0 0.0 0.0 136-137 7.925000000000001 0.0 0.0 0.0 0.0 138-139 8.7375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTAGGG 10 0.006830828 145.0 8 TTATACA 10 0.006830828 145.0 8 >>END_MODULE Read 969347 spots for SRR13695441.sra Written 969347 spots for SRR13695441.sra Read 969347 spots for SRR13695441.sra Written 969347 spots for SRR13695441.sra Read 969347 spots for SRR13695441.sra Written 969347 spots for SRR13695441.sra Read 969347 spots for SRR13695441.sra Written 969347 spots for SRR13695441.sra Read 969347 spots for SRR13695441.sra Written 969347 spots for SRR13695441.sra Read 969347 spots for SRR13695441.sra Written 969347 spots for SRR13695441.sra Read 969347 spots for SRR13695441.sra Written 969347 spots for SRR13695441.sra Read 969347 spots for SRR13695441.sra Written 969347 spots for SRR13695441.sra Read 969347 spots for SRR13695441.sra Written 969347 spots for SRR13695441.sra Read 969347 spots for SRR13695441.sra Written 969347 spots for SRR13695441.sra Read 969347 spots for SRR13695441.sra Written 969347 spots for SRR13695441.sra Read 969347 spots for SRR13695441.sra Written 969347 spots for SRR13695441.sra Read 969347 spots for SRR13695441.sra Written 969347 spots for SRR13695441.sra Read 969347 spots for SRR13695441.sra Written 969347 spots for SRR13695441.sra Read 969347 spots for SRR13695441.sra Written 969347 spots for SRR13695441.sra Read 969359 spots for SRR13695441.sra Written 969359 spots for SRR13695441.sra Read 969347 spots for SRR13695441.sra Written 969347 spots for SRR13695441.sra Read 969347 spots for SRR13695441.sra Written 969347 spots for SRR13695441.sra Read 969347 spots for SRR13695441.sra Written 969347 spots for SRR13695441.sra Read 969347 spots for SRR13695441.sra Written 969347 spots for SRR13695441.sra SRR ids: ['SRR13695441.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_of_icvwu SRR13695441.sra spots: 19386952 blocks: [[1, 969347], [969348, 1938694], [1938695, 2908041], [2908042, 3877388], [3877389, 4846735], [4846736, 5816082], [5816083, 6785429], [6785430, 7754776], [7754777, 8724123], [8724124, 9693470], [9693471, 10662817], [10662818, 11632164], [11632165, 12601511], [12601512, 13570858], [13570859, 14540205], [14540206, 15509552], [15509553, 16478899], [16478900, 17448246], [17448247, 18417593], [18417594, 19386952]] SRR13695441 file size 6566834 SRR13695441 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695441 SRR13695441_1.fastq SRR13695441_2.fastq Input file: SRR13695441_1.fastq Paired file: SRR13695441_2.fastq trimmed: SRR13695441-trimmed-pair1.fastq, SRR13695441-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 03:26:29 2025 >> started Wed Feb 12 03:26:52 2025 >> done (23.758s) 19386952 read pairs processed; of these: 141 ( 0.00%) short read pairs filtered out after trimming by size control 3667 ( 0.02%) empty read pairs filtered out after trimming by size control 19383144 (99.98%) read pairs available; of these: 2130838 (10.99%) trimmed read pairs available after processing 17252306 (89.01%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 1 0.00% 20 3 0.00% 21 0 0.00% 22 7 0.00% 23 1 0.00% 24 0 0.00% 25 1 0.00% 26 2 0.00% 27 2 0.00% 28 4 0.00% 29 8 0.00% 30 2 0.00% 31 6 0.00% 32 2 0.00% 33 8 0.00% 34 7 0.00% 35 8 0.00% 36 6 0.00% 37 17 0.00% 38 23 0.00% 39 16 0.00% 40 15 0.00% 41 28 0.00% 42 27 0.00% 43 17 0.00% 44 26 0.00% 45 38 0.00% 46 31 0.00% 47 62 0.00% 48 31 0.00% 49 70 0.00% 50 84 0.00% 51 97 0.00% 52 125 0.00% 53 125 0.00% 54 119 0.00% 55 132 0.00% 56 150 0.00% 57 193 0.00% 58 246 0.00% 59 276 0.00% 60 272 0.00% 61 356 0.00% 62 445 0.00% 63 430 0.00% 64 520 0.00% 65 522 0.00% 66 614 0.00% 67 741 0.00% 68 813 0.00% 69 908 0.00% 70 1045 0.01% 71 1070 0.01% 72 1445 0.01% 73 1716 0.01% 74 1879 0.01% 75 1944 0.01% 76 2199 0.01% 77 2523 0.01% 78 2790 0.01% 79 3103 0.02% 80 3376 0.02% 81 3746 0.02% 82 4311 0.02% 83 4715 0.02% 84 5524 0.03% 85 6040 0.03% 86 6116 0.03% 87 6821 0.04% 88 7488 0.04% 89 7747 0.04% 90 8369 0.04% 91 9126 0.05% 92 9785 0.05% 93 10623 0.05% 94 11315 0.06% 95 12058 0.06% 96 12719 0.07% 97 13526 0.07% 98 13831 0.07% 99 14462 0.07% 100 15628 0.08% 101 16017 0.08% 102 16936 0.09% 103 18045 0.09% 104 18938 0.10% 105 19784 0.10% 106 20264 0.10% 107 21028 0.11% 108 21813 0.11% 109 22966 0.12% 110 22944 0.12% 111 24881 0.13% 112 25513 0.13% 113 26115 0.13% 114 27128 0.14% 115 28042 0.14% 116 29846 0.15% 117 30687 0.16% 118 31834 0.16% 119 31752 0.16% 120 33177 0.17% 121 33744 0.17% 122 34650 0.18% 123 35562 0.18% 124 37202 0.19% 125 38006 0.20% 126 39595 0.20% 127 39771 0.21% 128 41280 0.21% 129 41469 0.21% 130 42885 0.22% 131 43252 0.22% 132 43834 0.23% 133 45667 0.24% 134 45718 0.24% 135 47349 0.24% 136 48000 0.25% 137 49164 0.25% 138 49086 0.25% 139 51472 0.27% 140 51815 0.27% 141 52701 0.27% 142 53432 0.28% 143 54299 0.28% 144 55481 0.29% 145 56671 0.29% 146 57062 0.29% 147 58207 0.30% 148 60269 0.31% 149 60068 0.31% 150 60740 0.31% 151 17252306 89.01% 19383144 reads passed initial QC criterion=sequence-density sequence-density=0.36 sequence-density-rank=1 fanout-score=2.14 fanout-score-rank=30 prefix-density=0.38 prefix-fanout=2.0 sequence=TGAGATGCCTCAGTGCATCCAAACATGGGTAGCTTCCACATAGTCCAGTAGCGTCCATCATAGTACCCTGGTGAGCTGTGGTGCTCACGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTTCGAGCGAAGAAGGTAATCAATTTCCTTGGCCAATTCCTCCTCTGTGAGATCTGGAAGGTAAGAAAGAGTCTCGAACTTCTTCAATCCAGTTGGTGGCCACACCTGCATGCATTGAACTCGTCCACCATTGCTTGCAATGGAAGTAATGTCATTAGCCTTTCTGGTACTGACTGGGAAAGCTGCGGCAGACTTGAGACCATTGAATGGTGCCACCAT criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=495.82 fanout-score-rank=1 prefix-density=0.25 prefix-fanout=17.9 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAG criterion=sequence-density sequence-density=0.68 sequence-density-rank=1 fanout-score=2.06 fanout-score-rank=34 prefix-density=0.68 prefix-fanout=2.1 sequence=CTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAGTGAACAAACAAAAGTGTCCGTGAGTGTCTTTATCTTTGTGTCTTCTTCA criterion=fanout-score sequence-density=0.12 sequence-density-rank=16 fanout-score=35.97 fanout-score-rank=1 prefix-density=0.32 prefix-fanout=13.2 sequence=AAAGAAAAGAAAA SRR13695441 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 03:27:30 Started mapping on | Feb 12 03:27:30 Finished on | Feb 12 03:29:35 Mapping speed, Million of reads per hour | 558.23 Number of input reads | 19383144 Average input read length | 296 UNIQUE READS: Uniquely mapped reads number | 18049326 Uniquely mapped reads % | 93.12% Average mapped length | 294.96 Number of splices: Total | 17401201 Number of splices: Annotated (sjdb) | 17001537 Number of splices: GT/AG | 17045980 Number of splices: GC/AG | 276968 Number of splices: AT/AC | 11785 Number of splices: Non-canonical | 66468 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 2.85 Insertion rate per base | 0.02% Insertion average length | 2.40 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 470132 % of reads mapped to multiple loci | 2.43% Number of reads mapped to too many loci | 88351 % of reads mapped to too many loci | 0.46% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.85% % of reads unmapped: other | 0.15% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 863946 863946 863946 N_multimapping 470132 470132 470132 N_noFeature 777821 17656832 1011915 N_ambiguous 268223 1627 108657 UnstrandedReadsAssigned:17003282 PositiveStrandReadsAssigned:390867 NegativeStrandReadsAssigned:16928754 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695441 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695441-trimmed-pair1.fastq SRR13695441-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 19,383,144 reads, 17,014,290 reads pseudoaligned [quant] estimated average fragment length: 251.294 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,054 rounds 52401 SRR13695441.ke.tsv 34699 SRR13695441.se.tsv 87100 total ==> SRR13695441.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1767.71 700 22.0433 Potri.005G024800.1.v4.1 1035 784.706 260 18.444 Potri.004G059700.1.v4.1 961 710.873 13 1.01798 Potri.007G009000.2.v4.1 1416 1165.71 0 0 Potri.003G141000.2.v4.1 2943 2692.71 733.049 15.1542 Potri.016G087400.1.v4.1 270 84.9011 1005 658.934 Potri.015G069301.1.v4.1 564 324.039 0 0 Potri.010G195200.1.v4.1 1773 1522.71 20 0.731144 Potri.012G127500.1.v4.1 977 726.772 123 9.42098 ==> SRR13695441.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 314 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 326 Potri.001G212900.v4.1 6 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 3 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 8 SRR13695441 completed mapping pipeline successfully