Starting /dee2/code/volunteer_pipeline.sh SRR13695442 current disk space = 3048986464256 free memory = 1579579364 SRR13695442 SRAfilesize f3a72e531583168a87e39cfd602765a0 SRR13695442.sra SRR13695442.sra file validated SRR13695442 is paired end SRR13695442 is conventional basespace SRR13695442 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695442_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5655 37.0 37.0 37.0 37.0 37.0 2 36.181 37.0 37.0 37.0 37.0 37.0 3 36.5775 37.0 37.0 37.0 37.0 37.0 4 36.615 37.0 37.0 37.0 37.0 37.0 5 36.6365 37.0 37.0 37.0 37.0 37.0 6 36.6205 37.0 37.0 37.0 37.0 37.0 7 36.453 37.0 37.0 37.0 37.0 37.0 8 36.5565 37.0 37.0 37.0 37.0 37.0 9 36.535 37.0 37.0 37.0 37.0 37.0 10-14 36.556799999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.5158 37.0 37.0 37.0 37.0 37.0 20-24 36.5037 37.0 37.0 37.0 37.0 37.0 25-29 36.4428 37.0 37.0 37.0 37.0 37.0 30-34 36.3827 37.0 37.0 37.0 37.0 37.0 35-39 36.3428 37.0 37.0 37.0 37.0 37.0 40-44 36.3929 37.0 37.0 37.0 37.0 37.0 45-49 36.25279999999999 37.0 37.0 37.0 37.0 37.0 50-54 36.196600000000004 37.0 37.0 37.0 37.0 37.0 55-59 36.281 37.0 37.0 37.0 37.0 37.0 60-64 36.227599999999995 37.0 37.0 37.0 37.0 37.0 65-69 36.1457 37.0 37.0 37.0 37.0 37.0 70-74 36.2347 37.0 37.0 37.0 37.0 37.0 75-79 36.2494 37.0 37.0 37.0 37.0 37.0 80-84 36.13719999999999 37.0 37.0 37.0 37.0 37.0 85-89 36.164 37.0 37.0 37.0 37.0 37.0 90-94 36.107499999999995 37.0 37.0 37.0 37.0 37.0 95-99 36.0903 37.0 37.0 37.0 37.0 37.0 100-104 36.112 37.0 37.0 37.0 37.0 37.0 105-109 36.057900000000004 37.0 37.0 37.0 37.0 37.0 110-114 35.980599999999995 37.0 37.0 37.0 37.0 37.0 115-119 36.041 37.0 37.0 37.0 37.0 37.0 120-124 35.9718 37.0 37.0 37.0 37.0 37.0 125-129 35.9054 37.0 37.0 37.0 37.0 37.0 130-134 35.8945 37.0 37.0 37.0 37.0 37.0 135-139 35.8808 37.0 37.0 37.0 37.0 37.0 140-144 35.7696 37.0 37.0 37.0 37.0 37.0 145-149 35.5792 37.0 37.0 37.0 37.0 37.0 150-151 35.41075 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 2.0 24 1.0 25 5.0 26 11.0 27 10.0 28 8.0 29 22.0 30 17.0 31 46.0 32 62.0 33 93.0 34 145.0 35 324.0 36 2918.0 37 336.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 26.174999999999997 10.674999999999999 15.075 48.075 2 19.919517102615693 14.66297786720322 35.7897384305835 29.62776659959759 3 19.7 16.900000000000002 23.75 39.65 4 22.900000000000002 23.799999999999997 21.175 32.125 5 23.3 31.624999999999996 24.775 20.3 6 21.4 33.75 23.474999999999998 21.375 7 16.025 27.400000000000002 39.6 16.975 8 18.575 26.075 32.6 22.75 9 19.8 23.35 35.65 21.2 10-14 20.015 29.635 27.36 22.99 15-19 19.675 28.095 28.715000000000003 23.515 20-24 19.865 28.38 27.884999999999998 23.87 25-29 20.565 29.509999999999998 26.565 23.36 30-34 19.905 28.694999999999997 26.974999999999998 24.425 35-39 20.07 28.405 27.675 23.849999999999998 40-44 19.61 29.675 27.62 23.095 45-49 20.31 28.585 27.33 23.775 50-54 19.975 28.665000000000003 27.51 23.849999999999998 55-59 20.74 28.1 27.41 23.75 60-64 20.09 28.660000000000004 27.955000000000002 23.294999999999998 65-69 20.22 28.565 28.549999999999997 22.665 70-74 20.645 28.139999999999997 28.15 23.064999999999998 75-79 20.315 27.99 28.175 23.52 80-84 20.855 29.075 26.705000000000002 23.365 85-89 21.560000000000002 28.515 27.32 22.605 90-94 21.13 28.52 27.51 22.84 95-99 21.029999999999998 28.485 27.134999999999998 23.35 100-104 20.105 29.104999999999997 27.26 23.53 105-109 20.935000000000002 28.349999999999998 27.465 23.25 110-114 20.815 28.794999999999998 26.825 23.565 115-119 20.849999999999998 27.295 28.499999999999996 23.355 120-124 20.61 26.6 28.82 23.97 125-129 21.7 27.639999999999997 27.22 23.44 130-134 21.005 28.005000000000003 27.125 23.865 135-139 21.265 28.63 27.185 22.919999999999998 140-144 21.72 27.42 27.58 23.28 145-149 21.97 27.76 26.729999999999997 23.54 150-151 22.475 27.787499999999998 25.9875 23.75 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.0 16 0.0 17 0.5 18 0.5 19 0.5 20 0.5 21 0.0 22 0.5 23 1.5 24 2.5 25 4.5 26 5.0 27 3.5 28 7.0 29 19.0 30 25.5 31 26.5 32 31.5 33 40.5 34 46.5 35 63.5 36 97.0 37 109.0 38 128.5 39 161.5 40 202.5 41 222.5 42 221.0 43 235.5 44 243.0 45 268.5 46 279.0 47 264.0 48 231.0 49 191.5 50 193.0 51 155.5 52 110.5 53 104.0 54 69.5 55 48.5 56 47.0 57 33.5 58 27.0 59 24.0 60 14.0 61 9.5 62 6.5 63 2.5 64 2.5 65 2.0 66 3.0 67 3.5 68 2.0 69 2.5 70 1.5 71 0.5 72 1.5 73 1.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.6 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.15 #Duplication Level Percentage of deduplicated Percentage of total 1 71.38664710198093 48.65 2 17.24137931034483 23.5 3 7.079970652971386 14.475 4 2.93470286133529 8.0 5 0.6969919295671314 2.375 6 0.36683785766691124 1.5 7 0.22010271460014674 1.05 8 0.036683785766691124 0.2 9 0.0 0.0 >10 0.036683785766691124 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CACTGTTTTTTGGGAGTAATCCAAAACCTGCTATATTACATCTAACCATA 10 0.25 No Hit TGACACTCATTATCACCTTTTTTTTCCCTTCCACAAGTACTATAGCAGAA 8 0.2 No Hit CAGGTGGTAAAAAGGCAGTGGTAATGCCATTCTTGGTTACAGCTGAGGAT 7 0.17500000000000002 No Hit CCCTTGTCAGTTGAATTCACCAAGTAGTCCCCATTAGGCAGGTGTGAAAG 7 0.17500000000000002 No Hit GTCTACATAACCAGCTCCATAGTATACAGCAGCTTGGCCACTGCTATAAT 7 0.17500000000000002 No Hit CAATGTCCTTAAGATCTGCGGTAACTACCTTAAGATCAAAACCTCCGGCT 7 0.17500000000000002 No Hit CCCGGACCATTCAATCGGTAGGAGCGACGGGCGGTGTGTACAAAGGGCAG 7 0.17500000000000002 No Hit AGCCAATGACACCATTATATCATCAATCCCCTTCTCCATTCCTTTCAATC 7 0.17500000000000002 No Hit GTTCTCAATTGGACGGTCCCTCTCAGACCACACTGCAAGAGTGTCTTCAC 6 0.15 No Hit TTTCTTTCTAGCCTTGTCTCTATCTGTTTGTCTGGAACCATATGAAGAAG 6 0.15 No Hit CACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACAC 6 0.15 No Hit CTCCTCCACCTGGGCTTTCCTCAACAGCCCACCAGTGCTTCCACCAAAAA 6 0.15 No Hit CTCCAAATTACAGCCACACCCACCACACCTTTACAGATCAAGATTGACTT 6 0.15 No Hit GCCATCAGAGACTGCCATTGAATCATCAGTTACAAGTTGTCCATTCACTA 6 0.15 No Hit CACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTA 6 0.15 No Hit GCACAAAGTAACCCAAGATGGCCAACATAGCCAACCTGCCATTCTTCACC 6 0.15 No Hit CCTCAACCATCTCAACATTTCCAGCAGACTTAAAGAGATCAGCAAGCTGG 6 0.15 No Hit GATAAAACTGATGCACTGCACTTGACGAGTGTTGTCGAATCCAATGATAC 6 0.15 No Hit ACCAGTCAGAGCGGCTTGAAGTCATTGCAGTTATTCTGTTTTCTCCCTGA 5 0.125 No Hit ATTTGTTTGTGCAACCAAGATTTTTAGACACGGTTCTGGTGTTCTCTGCT 5 0.125 No Hit GAGCTTCAGCTACAGCTGGTTCTGTGGTGGCAGCAGGTGGTGATGCACCC 5 0.125 No Hit TCTCCATCAGAATTCAAATTGCCAGGAGGTTTGCCCCAGTTTTCTTCCAA 5 0.125 No Hit CTGCTGGTTTTGAGATGGTTAGGCCTTTTGGGAGGTTTTGCATGGAGAGA 5 0.125 No Hit CACCAGTCAAGTAGGATGGGGGCTCACCAGAGAACGGGCCCAAGTATTTA 5 0.125 No Hit GTTCTTGCACTTGGGTAGCAGTTGCCAGTTCCTCAGGAGCAGAATCACCA 5 0.125 No Hit CTGTTGATGCATACTCATTTCCTGACATGACTGCTCCTTTTCCTCCATCA 5 0.125 No Hit CCGTAGTATCTTGCACCTGGATATCCTTCACTGTACTTGTTGGTCATGAC 5 0.125 No Hit CCCATATTTTCGCCAGCGATATCCATCCTCTGACACTTTTTCCCGCATGA 5 0.125 No Hit CAGGAAATGAATTCTTCCTCTTCTCAAAAGCTTTAAGACTCAAGTACCCA 5 0.125 No Hit GTTCTGATCATTTTACATATTGATAAAGACAGCAGAAGCTCTCCATACTT 5 0.125 No Hit CAGTAGCGTCCATCATAGTACCCTGGTGAGCTGTGGTGCTCACGGTAGAC 5 0.125 No Hit ACCAAGCCCAGCTATGCTGTACAAGAGTGTCAGGACAATTATGTCAGGTG 5 0.125 No Hit CCGGGCAATAACTGATCGGATATCAAAGGCTTGCTTGTGGTCTATTGGTG 5 0.125 No Hit CCCCACTTGGCCCATGCATCCAGGTCTGGGAGTCACCACTACCATGAGAT 5 0.125 No Hit CCCGGAGCCAGCTAAAAAAACCACCAGGACCCTTCCCCATAAAATCATCA 5 0.125 No Hit TGTCTTTCCACGCCGACCAGCTAGAATAGCTGCCTCATTCAAGAGATTTG 5 0.125 No Hit TGCAAGCTGTTGGGTTCCCCATAGCCCGCCAGTTGCATCCAAAAATGCTT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0125 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.0625 0.0 0.0 0.0 0.0 80-81 0.1 0.0 0.0 0.0 0.0 82-83 0.1625 0.0 0.0 0.0 0.0 84-85 0.21250000000000002 0.0 0.0 0.0 0.0 86-87 0.2625 0.0 0.0 0.0 0.0 88-89 0.3 0.0 0.0 0.0 0.0 90-91 0.3 0.0 0.0 0.0 0.0 92-93 0.375 0.0 0.0 0.0 0.0 94-95 0.4375 0.0 0.0 0.0 0.0 96-97 0.55 0.0 0.0 0.0 0.0 98-99 0.6 0.0 0.0 0.0 0.0 100-101 0.8 0.0 0.0 0.0 0.0 102-103 0.925 0.0 0.0 0.0 0.0 104-105 1.1875 0.0 0.0 0.0 0.0 106-107 1.5750000000000002 0.0 0.0 0.0 0.0 108-109 1.7999999999999998 0.0 0.0 0.0 0.0 110-111 2.1125 0.0 0.0 0.0 0.0 112-113 2.375 0.0 0.0 0.0 0.0 114-115 2.5875 0.0 0.0 0.0 0.0 116-117 2.8125 0.0 0.0 0.0 0.0 118-119 3.1125 0.0 0.0 0.0 0.0 120-121 3.4000000000000004 0.0 0.0 0.0 0.0 122-123 3.9375 0.0 0.0 0.0 0.0 124-125 4.475 0.0 0.0 0.0 0.0 126-127 4.949999999999999 0.0 0.0 0.0 0.0 128-129 5.3 0.0 0.0 0.0 0.0 130-131 5.8875 0.0 0.0 0.0 0.0 132-133 6.1125 0.0 0.0 0.0 0.0 134-135 6.4875 0.0 0.0 0.0 0.0 136-137 7.1 0.0 0.0 0.0 0.0 138-139 7.625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGATGAT 10 0.006830828 145.0 7 ATCCAAT 10 0.006830828 145.0 6 >>END_MODULE SRR13695442 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695442_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.20825 37.0 37.0 37.0 37.0 37.0 2 36.2515 37.0 37.0 37.0 37.0 37.0 3 36.231 37.0 37.0 37.0 37.0 37.0 4 36.159 37.0 37.0 37.0 37.0 37.0 5 36.267 37.0 37.0 37.0 37.0 37.0 6 36.218 37.0 37.0 37.0 37.0 37.0 7 36.2815 37.0 37.0 37.0 37.0 37.0 8 36.3125 37.0 37.0 37.0 37.0 37.0 9 36.2235 37.0 37.0 37.0 37.0 37.0 10-14 36.2407 37.0 37.0 37.0 37.0 37.0 15-19 36.2064 37.0 37.0 37.0 37.0 37.0 20-24 36.20835 37.0 37.0 37.0 37.0 37.0 25-29 36.086349999999996 37.0 37.0 37.0 37.0 37.0 30-34 36.05669999999999 37.0 37.0 37.0 37.0 37.0 35-39 36.06395 37.0 37.0 37.0 37.0 37.0 40-44 36.04245 37.0 37.0 37.0 37.0 37.0 45-49 36.05265 37.0 37.0 37.0 37.0 37.0 50-54 35.982150000000004 37.0 37.0 37.0 37.0 37.0 55-59 35.99575 37.0 37.0 37.0 37.0 37.0 60-64 35.931650000000005 37.0 37.0 37.0 37.0 37.0 65-69 35.89405 37.0 37.0 37.0 37.0 37.0 70-74 35.88915000000001 37.0 37.0 37.0 37.0 37.0 75-79 35.87025 37.0 37.0 37.0 37.0 37.0 80-84 35.861149999999995 37.0 37.0 37.0 37.0 37.0 85-89 35.77445 37.0 37.0 37.0 37.0 37.0 90-94 35.83365 37.0 37.0 37.0 37.0 37.0 95-99 35.8309 37.0 37.0 37.0 37.0 37.0 100-104 35.81225 37.0 37.0 37.0 37.0 37.0 105-109 35.777 37.0 37.0 37.0 37.0 37.0 110-114 35.72665 37.0 37.0 37.0 37.0 37.0 115-119 35.70095 37.0 37.0 37.0 37.0 37.0 120-124 35.60295 37.0 37.0 37.0 37.0 37.0 125-129 35.58665 37.0 37.0 37.0 37.0 37.0 130-134 35.53845 37.0 37.0 37.0 37.0 37.0 135-139 35.532349999999994 37.0 37.0 37.0 37.0 37.0 140-144 35.37625 37.0 37.0 37.0 37.0 37.0 145-149 35.328250000000004 37.0 37.0 37.0 34.6 37.0 150-151 34.96625 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 1.0 13 0.0 14 0.0 15 3.0 16 2.0 17 4.0 18 1.0 19 1.0 20 3.0 21 1.0 22 1.0 23 2.0 24 2.0 25 9.0 26 11.0 27 10.0 28 17.0 29 19.0 30 32.0 31 30.0 32 58.0 33 111.0 34 221.0 35 603.0 36 2669.0 37 188.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 25.633944263118252 18.177253326638212 20.989204117499373 35.19959829274416 2 27.224999999999998 23.75 29.725 19.3 3 21.425 27.0 29.575000000000003 22.0 4 21.825 33.300000000000004 24.5 20.375 5 25.074999999999996 34.949999999999996 25.05 14.924999999999999 6 22.400000000000002 35.25 24.025 18.325 7 20.375 20.25 38.9 20.474999999999998 8 23.075000000000003 25.974999999999998 26.05 24.9 9 21.6 23.7 32.35 22.35 10-14 22.495 29.39 26.575 21.54 15-19 22.634999999999998 27.29 28.035 22.040000000000003 20-24 21.992697444105435 28.765067773720805 27.36957935277347 21.87265542940029 25-29 22.39679759819865 28.32124093069802 27.775831873905428 21.506129597197898 30-34 22.021010505252626 29.41970985492746 27.20360180090045 21.35567783891946 35-39 22.627919771920173 26.924423548241883 28.48997149002151 21.957685189816438 40-44 22.61243684026214 28.075441492821053 28.065435989794384 21.246685677122418 45-49 22.405082287029163 27.572407583412534 28.04762142964334 21.974888699914963 50-54 22.597909268243885 27.659680888310913 27.89476316710849 21.84764667633672 55-59 22.78208656492369 27.89592194145609 28.101075806855143 21.220915686765075 60-64 22.41284449557345 27.72970539688891 28.630020507177512 21.227429600360125 65-69 23.108466269940493 27.01905285792869 28.3842576386458 21.488223233485023 70-74 23.252439329497125 26.93520140105079 27.685764323242434 22.126594946209657 75-79 21.837010355695632 28.735804692580917 26.944819650807943 22.482365300915504 80-84 23.29082270567642 28.30207551887972 27.286821705426355 21.120280070017504 85-89 22.95992395056787 28.063241106719367 27.27272727272727 21.70410766998549 90-94 23.51322963037063 28.890111539038664 26.879407792727456 20.717251037863253 95-99 23.4370311093328 28.473542062618783 27.463238971691506 20.626187856356907 100-104 23.39254440830623 28.15111333500125 27.72079059294471 20.735551663747813 105-109 23.691584108876214 28.615030521364954 26.498548984289 21.194836385469827 110-114 23.258140349122193 29.0401640574201 27.049467313559745 20.65222827989796 115-119 24.550958122779807 27.417821584029618 27.502876869965476 20.528343423225095 120-124 24.043032274205654 28.326244683512634 26.965223917938452 20.665499124343256 125-129 23.98058738179817 27.758042727773052 27.417821584029618 20.84354830639916 130-134 23.827870903177384 28.631473605203904 27.06529897423067 20.47535651738804 135-139 24.983737803352515 28.101075806855143 26.659994996247182 20.255191393545157 140-144 25.313859850947836 28.645025759015656 26.229180213074578 19.811934176961937 145-149 25.769326995246434 27.270452839629723 26.38478859144358 20.575431573680262 150-151 25.83187390542907 28.008506379784837 26.46985238929197 19.68976732549412 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.0 12 1.0 13 0.0 14 0.5 15 1.0 16 0.5 17 0.0 18 0.0 19 0.5 20 0.5 21 1.0 22 1.0 23 3.0 24 4.0 25 5.5 26 7.0 27 6.5 28 10.0 29 14.0 30 14.5 31 19.5 32 25.0 33 38.0 34 53.0 35 68.0 36 90.5 37 107.5 38 139.0 39 168.0 40 197.5 41 216.5 42 238.0 43 259.5 44 258.0 45 277.5 46 251.5 47 210.5 48 221.0 49 213.0 50 188.0 51 150.5 52 109.0 53 90.5 54 74.5 55 68.0 56 65.0 57 35.0 58 15.0 59 14.5 60 12.5 61 13.0 62 11.0 63 8.0 64 4.0 65 3.0 66 1.5 67 0.0 68 0.0 69 0.5 70 0.5 71 1.0 72 1.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.5 81 0.5 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 1.0 97 1.0 98 1.0 99 2.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42500000000000004 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.034999999999999996 25-29 0.075 30-34 0.05 35-39 0.034999999999999996 40-44 0.055 45-49 0.045 50-54 0.034999999999999996 55-59 0.075 60-64 0.034999999999999996 65-69 0.015 70-74 0.075 75-79 0.055 80-84 0.025 85-89 0.065 90-94 0.034999999999999996 95-99 0.03 100-104 0.075 105-109 0.06999999999999999 110-114 0.034999999999999996 115-119 0.065 120-124 0.075 125-129 0.065 130-134 0.075 135-139 0.075 140-144 0.034999999999999996 145-149 0.075 150-151 0.075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.72500000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 72.35358312113496 49.725 2 16.769734448890507 23.05 3 6.693343033830485 13.8 4 2.728264823572208 7.5 5 0.8002910149145145 2.75 6 0.29101491451436884 1.2 7 0.18188432157148052 0.8750000000000001 8 0.10913059294288831 0.6 9 0.0 0.0 >10 0.07275372862859221 0.5 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGAAATTCAAATCATCTCCCATTCTCAAGGAGCTACTTGAGCGATCCAA 10 0.25 No Hit AGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATC 10 0.25 No Hit AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 8 0.2 No Hit CACTCATGTTCCACCTGTGATTTTGAGGAATATAATGGAGAATCCGGCTT 8 0.2 No Hit ACCTAAGCAAAGAACAACTTCGTATTTAGTTCATCCATTTGCTTCATCAA 8 0.2 No Hit CTGATGGGCAGACTTTCGGTCCACCAAGCGGGGACTTGGTGCATGACCTT 7 0.17500000000000002 No Hit GGAAAGAAATGCTCATGCCTAAAGATCCCAATGCCACTGTCATTATGCTT 7 0.17500000000000002 No Hit TTCAGGCATCTTATTTCCTGATCCACTACCTATTTGCCAGTCAAACTGGT 7 0.17500000000000002 No Hit AGCGATTTGTCTGGTTAATTCCGTTAACGAACGAGACCTCAGCCTGCTAA 7 0.17500000000000002 No Hit TCTCTCTCTCCCTCTCTCTCTTCCAAAAAATATATCACCAAAACATTCCT 7 0.17500000000000002 No Hit TGTCATCCTCCTCTTCTAGATCTGTCAGAAAATCAGATCCTAAGGATTCT 6 0.15 No Hit GCCAACTGCCAGGCGCCAACTCAGCTTCTATCGGCACCTCTCCCTCACTC 6 0.15 No Hit GAGAGAGATATGGTTTTCAATTCATTTGGGGTCCGTGGACTTTCCACTGA 6 0.15 No Hit TCTGGAACTCTACAACTCGGGCAGAGCTGCTGAAATTTGTGGATCAGCAA 6 0.15 No Hit CGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCG 6 0.15 No Hit CCTACCATCGCTTAACCACCCAAACACCATAAAATCTTTCTACAGCAAAC 6 0.15 No Hit CTCGAATCTCCAATTCCTCGTGGCCATGGCAGCTCAAGCCTCTCTCTTTA 6 0.15 No Hit GACTCGGGTTGGAAACCCCGTTGGGTACAAGTTGGTTCCCGGTGGCACGG 6 0.15 No Hit TGGGTATTTGAAGAAAGGGAGTATGGCATCATTGGGAGGTGGTGCTGGCA 5 0.125 No Hit GTTTTCTCTCGAAGAACTCAAGGCTGCTGGTATTCCAAAGAAACTAGCTC 5 0.125 No Hit TGCAAAGTCCATTGTTGATTGGATAGCCTGGGCAGAACCACAATATAACT 5 0.125 No Hit CTCCGCTCCTCCTTTGAAGCTAAAACAAAATGGATGGATTGGGAATTTCG 5 0.125 No Hit GTTTAGTGGACTTGATGTACTTGGGGATGGAAATATTGCTGTCATGAAAT 5 0.125 No Hit TAGCCGATTCCTGTATAATTTGTTCTCCATTGAAGAACAAAACACCCGTT 5 0.125 No Hit GGTGGACGAGTTCAATGCATGCAGGTGTGGCCACCAACTGGATTGAAGAA 5 0.125 No Hit GCGGCAACTAACAGGGCGGACATTCTCGACTCTGCCTTGTTGAGGCCAGG 5 0.125 No Hit TCACAACTGATATAAAAGTGGACACAAACTCCAATCTTCTCACAACTATT 5 0.125 No Hit TGTCTTCTCTCCCTTATCCATTTGCACTGCTCGAGAATTGGCCGAGCGAG 5 0.125 No Hit CACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATC 5 0.125 No Hit TCTCGTCAGAGGAAGTTTCTCCCAAGGAATTGCAGAAAAGACAGAGCCTA 5 0.125 No Hit CAGCAACTTTCATTTCTCTCCAAGGAATTCATCTTCTTGCTCAGCTTGAT 5 0.125 No Hit GGGAGCACTGCATAGCTTATAAGCTTGTAAGAGATGGCTTCCTCCTCTAT 5 0.125 No Hit TTAGAGGTTGTTGACCCACAAATTGCTGATATTATTGAGCTCGAGAAAGC 5 0.125 No Hit GCGTTGGCACCAACTGTTGCAACCAATACAAACTCAACTTCTAAGCCCAC 5 0.125 No Hit AAGACATCAGGGGTTTCGGATTATTTTACTCAAGATGAATTGCATGGACT 5 0.125 No Hit CTTCTGGATTCAAGGTTCGTGATCTTGGAAAGATCCTCCATCTGGCTGGG 5 0.125 No Hit CGGTTCTGAAAGAACTAGAAAGAGATGGCTACAATGTTGAGGGCCTTCCA 5 0.125 No Hit ATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAAT 5 0.125 No Hit TACTGTGGAAGGAGTGGAAATTAAGCTATGGCAGGTACCTGAAACCCTGC 5 0.125 No Hit CAGCAAAGAAAGTTAAGGAGAAAGCAATCAGACTGAAAAGGTCTTTCAAG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0125 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.0625 0.0 0.0 0.0 0.0 80-81 0.1 0.0 0.0 0.0 0.0 82-83 0.1625 0.0 0.0 0.0 0.0 84-85 0.21250000000000002 0.0 0.0 0.0 0.0 86-87 0.2625 0.0 0.0 0.0 0.0 88-89 0.3 0.0 0.0 0.0 0.0 90-91 0.3 0.0 0.0 0.0 0.0 92-93 0.3875 0.0 0.0 0.0 0.0 94-95 0.4625 0.0 0.0 0.0 0.0 96-97 0.575 0.0 0.0 0.0 0.0 98-99 0.625 0.0 0.0 0.0 0.0 100-101 0.825 0.0 0.0 0.0 0.0 102-103 0.95 0.0 0.0 0.0 0.0 104-105 1.2125 0.0 0.0 0.0 0.0 106-107 1.6 0.0 0.0 0.0 0.0 108-109 1.8250000000000002 0.0 0.0 0.0 0.0 110-111 2.1375 0.0 0.0 0.0 0.0 112-113 2.4 0.0 0.0 0.0 0.0 114-115 2.6125 0.0 0.0 0.0 0.0 116-117 2.8375 0.0 0.0 0.0 0.0 118-119 3.15 0.0 0.0 0.0 0.0 120-121 3.45 0.0 0.0 0.0 0.0 122-123 3.9875 0.0 0.0 0.0 0.0 124-125 4.525 0.0 0.0 0.0 0.0 126-127 5.0 0.0 0.0 0.0 0.0 128-129 5.3375 0.0 0.0 0.0 0.0 130-131 5.9125 0.0 0.0 0.0 0.0 132-133 6.1375 0.0 0.0 0.0 0.0 134-135 6.475 0.0 0.0 0.0 0.0 136-137 7.074999999999999 0.0 0.0 0.0 0.0 138-139 7.6 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 1042178 spots for SRR13695442.sra Written 1042178 spots for SRR13695442.sra Read 1042178 spots for SRR13695442.sra Written 1042178 spots for SRR13695442.sra Read 1042178 spots for SRR13695442.sra Written 1042178 spots for SRR13695442.sra Read 1042178 spots for SRR13695442.sra Written 1042178 spots for SRR13695442.sra Read 1042178 spots for SRR13695442.sra Written 1042178 spots for SRR13695442.sra Read 1042178 spots for SRR13695442.sra Written 1042178 spots for SRR13695442.sra Read 1042178 spots for SRR13695442.sra Written 1042178 spots for SRR13695442.sra Read 1042178 spots for SRR13695442.sra Written 1042178 spots for SRR13695442.sra Read 1042178 spots for SRR13695442.sra Written 1042178 spots for SRR13695442.sra Read 1042178 spots for SRR13695442.sra Written 1042178 spots for SRR13695442.sra Read 1042178 spots for SRR13695442.sra Written 1042178 spots for SRR13695442.sra Read 1042178 spots for SRR13695442.sra Written 1042178 spots for SRR13695442.sra Read 1042178 spots for SRR13695442.sra Written 1042178 spots for SRR13695442.sra Read 1042178 spots for SRR13695442.sra Written 1042178 spots for SRR13695442.sra Read 1042178 spots for SRR13695442.sra Written 1042178 spots for SRR13695442.sra Read 1042178 spots for SRR13695442.sra Written 1042178 spots for SRR13695442.sra Read 1042178 spots for SRR13695442.sra Written 1042178 spots for SRR13695442.sra Read 1042186 spots for SRR13695442.sra Written 1042186 spots for SRR13695442.sra Read 1042178 spots for SRR13695442.sra Written 1042178 spots for SRR13695442.sra Read 1042178 spots for SRR13695442.sra Written 1042178 spots for SRR13695442.sra SRR ids: ['SRR13695442.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_hz3y423m SRR13695442.sra spots: 20843568 blocks: [[1, 1042178], [1042179, 2084356], [2084357, 3126534], [3126535, 4168712], [4168713, 5210890], [5210891, 6253068], [6253069, 7295246], [7295247, 8337424], [8337425, 9379602], [9379603, 10421780], [10421781, 11463958], [11463959, 12506136], [12506137, 13548314], [13548315, 14590492], [14590493, 15632670], [15632671, 16674848], [16674849, 17717026], [17717027, 18759204], [18759205, 19801382], [19801383, 20843568]] SRR13695442 file size 7061855 SRR13695442 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695442 SRR13695442_1.fastq SRR13695442_2.fastq Input file: SRR13695442_1.fastq Paired file: SRR13695442_2.fastq trimmed: SRR13695442-trimmed-pair1.fastq, SRR13695442-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 03:46:55 2025 >> started Wed Feb 12 03:47:17 2025 >> done (22.370s) 20843568 read pairs processed; of these: 144 ( 0.00%) short read pairs filtered out after trimming by size control 45928 ( 0.22%) empty read pairs filtered out after trimming by size control 20797496 (99.78%) read pairs available; of these: 2056242 ( 9.89%) trimmed read pairs available after processing 18741254 (90.11%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 1 0.00% 20 4 0.00% 21 3 0.00% 22 4 0.00% 23 5 0.00% 24 1 0.00% 25 3 0.00% 26 5 0.00% 27 3 0.00% 28 5 0.00% 29 6 0.00% 30 3 0.00% 31 2 0.00% 32 10 0.00% 33 11 0.00% 34 13 0.00% 35 8 0.00% 36 13 0.00% 37 12 0.00% 38 12 0.00% 39 15 0.00% 40 14 0.00% 41 31 0.00% 42 27 0.00% 43 34 0.00% 44 26 0.00% 45 27 0.00% 46 31 0.00% 47 41 0.00% 48 69 0.00% 49 51 0.00% 50 70 0.00% 51 67 0.00% 52 102 0.00% 53 80 0.00% 54 123 0.00% 55 123 0.00% 56 114 0.00% 57 129 0.00% 58 149 0.00% 59 187 0.00% 60 238 0.00% 61 282 0.00% 62 298 0.00% 63 370 0.00% 64 365 0.00% 65 448 0.00% 66 445 0.00% 67 487 0.00% 68 519 0.00% 69 605 0.00% 70 794 0.00% 71 830 0.00% 72 967 0.00% 73 1140 0.01% 74 1251 0.01% 75 1444 0.01% 76 1530 0.01% 77 1680 0.01% 78 1897 0.01% 79 2184 0.01% 80 2346 0.01% 81 2656 0.01% 82 3044 0.01% 83 3420 0.02% 84 4081 0.02% 85 4359 0.02% 86 4730 0.02% 87 5125 0.02% 88 5664 0.03% 89 6093 0.03% 90 6274 0.03% 91 7165 0.03% 92 7484 0.04% 93 8209 0.04% 94 9062 0.04% 95 9937 0.05% 96 10266 0.05% 97 11080 0.05% 98 11753 0.06% 99 11974 0.06% 100 13192 0.06% 101 13289 0.06% 102 14305 0.07% 103 15222 0.07% 104 16407 0.08% 105 17271 0.08% 106 18377 0.09% 107 19206 0.09% 108 19729 0.09% 109 20687 0.10% 110 21288 0.10% 111 22431 0.11% 112 23040 0.11% 113 24003 0.12% 114 25267 0.12% 115 27076 0.13% 116 27783 0.13% 117 28719 0.14% 118 30169 0.15% 119 30422 0.15% 120 31192 0.15% 121 32770 0.16% 122 33219 0.16% 123 34286 0.16% 124 35353 0.17% 125 36950 0.18% 126 37723 0.18% 127 39392 0.19% 128 40687 0.20% 129 40858 0.20% 130 42452 0.20% 131 42734 0.21% 132 43444 0.21% 133 44923 0.22% 134 45561 0.22% 135 46487 0.22% 136 48139 0.23% 137 50193 0.24% 138 50737 0.24% 139 52243 0.25% 140 53777 0.26% 141 54026 0.26% 142 55227 0.27% 143 55783 0.27% 144 56984 0.27% 145 57844 0.28% 146 59791 0.29% 147 60121 0.29% 148 62704 0.30% 149 63995 0.31% 150 64634 0.31% 151 18741254 90.11% 20797496 reads passed initial QC criterion=sequence-density sequence-density=0.39 sequence-density-rank=1 fanout-score=1.94 fanout-score-rank=28 prefix-density=0.40 prefix-fanout=1.9 sequence=CGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTTCGAGCGAAGAAGGT criterion=fanout-score sequence-density=0.01 sequence-density-rank=28 fanout-score=479.32 fanout-score-rank=1 prefix-density=0.25 prefix-fanout=16.4 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGAGTATGAATGTGTTCTCTGTG criterion=sequence-density sequence-density=0.54 sequence-density-rank=1 fanout-score=2.12 fanout-score-rank=26 prefix-density=0.55 prefix-fanout=2.1 sequence=TGTAAGAGATGGCTTCCTC criterion=fanout-score sequence-density=0.23 sequence-density-rank=11 fanout-score=13.10 fanout-score-rank=1 prefix-density=0.47 prefix-fanout=6.4 sequence=AAGAAAGCTTACCCTAAC SRR13695442 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 03:48:03 Started mapping on | Feb 12 03:48:04 Finished on | Feb 12 03:50:49 Mapping speed, Million of reads per hour | 453.76 Number of input reads | 20797496 Average input read length | 297 UNIQUE READS: Uniquely mapped reads number | 19329110 Uniquely mapped reads % | 92.94% Average mapped length | 296.16 Number of splices: Total | 19712261 Number of splices: Annotated (sjdb) | 19309408 Number of splices: GT/AG | 19313051 Number of splices: GC/AG | 316599 Number of splices: AT/AC | 10941 Number of splices: Non-canonical | 71670 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.03% Deletion average length | 2.98 Insertion rate per base | 0.02% Insertion average length | 2.39 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 501469 % of reads mapped to multiple loci | 2.41% Number of reads mapped to too many loci | 162039 % of reads mapped to too many loci | 0.78% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.70% % of reads unmapped: other | 0.17% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 967141 967141 967141 N_multimapping 501469 501469 501469 N_noFeature 732189 18842026 1003343 N_ambiguous 330862 1846 113656 UnstrandedReadsAssigned:18266059 PositiveStrandReadsAssigned:485238 NegativeStrandReadsAssigned:18212111 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695442 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695442-trimmed-pair1.fastq SRR13695442-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 20,797,496 reads, 18,277,816 reads pseudoaligned [quant] estimated average fragment length: 254.582 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,070 rounds 52401 SRR13695442.ke.tsv 34699 SRR13695442.se.tsv 87100 total ==> SRR13695442.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1764.42 644 16.6098 Potri.005G024800.1.v4.1 1035 781.418 380 22.1299 Potri.004G059700.1.v4.1 961 707.562 2 0.128631 Potri.007G009000.2.v4.1 1416 1162.42 0 0 Potri.003G141000.2.v4.1 2943 2689.42 884 14.958 Potri.016G087400.1.v4.1 270 80.7941 994 559.87 Potri.015G069301.1.v4.1 564 319.484 0 0 Potri.010G195200.1.v4.1 1773 1519.42 137 4.10321 Potri.012G127500.1.v4.1 977 723.491 140 8.80593 ==> SRR13695442.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 95 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 283 Potri.001G212900.v4.1 923 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 1 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR13695442 completed mapping pipeline successfully