Starting /dee2/code/volunteer_pipeline.sh SRR13695443 current disk space = 3048953217024 free memory = 736400344 SRR13695443 SRAfilesize 37e363696df7e3fcf4973eacf2b2acc6 SRR13695443.sra SRR13695443.sra file validated SRR13695443 is paired end SRR13695443 is conventional basespace SRR13695443 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695443_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5135 37.0 37.0 37.0 37.0 37.0 2 36.326 37.0 37.0 37.0 37.0 37.0 3 36.581 37.0 37.0 37.0 37.0 37.0 4 36.641 37.0 37.0 37.0 37.0 37.0 5 36.5875 37.0 37.0 37.0 37.0 37.0 6 36.4975 37.0 37.0 37.0 37.0 37.0 7 36.5405 37.0 37.0 37.0 37.0 37.0 8 36.5745 37.0 37.0 37.0 37.0 37.0 9 36.51 37.0 37.0 37.0 37.0 37.0 10-14 36.5635 37.0 37.0 37.0 37.0 37.0 15-19 36.5618 37.0 37.0 37.0 37.0 37.0 20-24 36.525999999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.4918 37.0 37.0 37.0 37.0 37.0 30-34 36.458600000000004 37.0 37.0 37.0 37.0 37.0 35-39 36.456599999999995 37.0 37.0 37.0 37.0 37.0 40-44 36.422200000000004 37.0 37.0 37.0 37.0 37.0 45-49 36.4119 37.0 37.0 37.0 37.0 37.0 50-54 36.3809 37.0 37.0 37.0 37.0 37.0 55-59 36.38870000000001 37.0 37.0 37.0 37.0 37.0 60-64 36.339000000000006 37.0 37.0 37.0 37.0 37.0 65-69 36.312200000000004 37.0 37.0 37.0 37.0 37.0 70-74 36.3159 37.0 37.0 37.0 37.0 37.0 75-79 36.301 37.0 37.0 37.0 37.0 37.0 80-84 36.21 37.0 37.0 37.0 37.0 37.0 85-89 36.2779 37.0 37.0 37.0 37.0 37.0 90-94 36.1623 37.0 37.0 37.0 37.0 37.0 95-99 36.14379999999999 37.0 37.0 37.0 37.0 37.0 100-104 36.0937 37.0 37.0 37.0 37.0 37.0 105-109 36.1137 37.0 37.0 37.0 37.0 37.0 110-114 36.0706 37.0 37.0 37.0 37.0 37.0 115-119 36.152100000000004 37.0 37.0 37.0 37.0 37.0 120-124 36.006600000000006 37.0 37.0 37.0 37.0 37.0 125-129 35.962199999999996 37.0 37.0 37.0 37.0 37.0 130-134 35.9294 37.0 37.0 37.0 37.0 37.0 135-139 35.88779999999999 37.0 37.0 37.0 37.0 37.0 140-144 35.8235 37.0 37.0 37.0 37.0 37.0 145-149 35.625099999999996 37.0 37.0 37.0 37.0 37.0 150-151 35.585499999999996 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 0.0 22 0.0 23 0.0 24 1.0 25 0.0 26 1.0 27 12.0 28 10.0 29 21.0 30 31.0 31 26.0 32 44.0 33 82.0 34 147.0 35 317.0 36 2936.0 37 371.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.5 11.275 9.975000000000001 44.25 2 20.90452261306533 14.020100502512564 35.85427135678392 29.22110552763819 3 17.724999999999998 17.549999999999997 27.1 37.625 4 22.325 23.200000000000003 23.95 30.525000000000002 5 23.75 31.05 24.2 21.0 6 20.325 35.275 22.85 21.55 7 14.549999999999999 27.6 41.925000000000004 15.925 8 17.375 26.325 32.725 23.575 9 19.900000000000002 22.575 34.4 23.125 10-14 18.88 30.354999999999997 28.095 22.67 15-19 19.650000000000002 27.985 28.23 24.135 20-24 20.45 28.13 28.585 22.835 25-29 19.64 28.810000000000002 27.665 23.885 30-34 19.384999999999998 28.599999999999998 28.275 23.74 35-39 19.72 27.73 28.105000000000004 24.445 40-44 19.855 27.694999999999997 28.38 24.07 45-49 20.205000000000002 27.575 27.985 24.235 50-54 20.18 28.125 27.625 24.07 55-59 20.115 28.285 27.865000000000002 23.735 60-64 20.925 28.694999999999997 26.965 23.415 65-69 20.849999999999998 28.48 27.529999999999998 23.14 70-74 20.26 28.22 27.425 24.095 75-79 20.474999999999998 27.985 28.7 22.84 80-84 20.674999999999997 28.299999999999997 27.92 23.105 85-89 20.585 28.07 27.08 24.265 90-94 19.975 29.65 27.85 22.525000000000002 95-99 20.544999999999998 27.975 27.900000000000002 23.580000000000002 100-104 20.665 28.345 27.084999999999997 23.905 105-109 20.810000000000002 27.565 27.79 23.835 110-114 20.06 28.225 27.72 23.995 115-119 20.745 27.705000000000002 28.32 23.23 120-124 20.645 27.894999999999996 26.939999999999998 24.52 125-129 21.645 28.185 26.87 23.3 130-134 21.245 28.63 27.045 23.080000000000002 135-139 20.945 27.42 27.51 24.125 140-144 21.584999999999997 27.55 27.26 23.605 145-149 21.45 27.87 27.084999999999997 23.595 150-151 22.7375 27.0625 27.150000000000002 23.05 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.5 23 1.0 24 2.5 25 6.5 26 6.0 27 3.5 28 12.5 29 14.5 30 17.0 31 32.0 32 32.5 33 38.0 34 61.0 35 79.0 36 100.0 37 120.0 38 132.0 39 155.0 40 201.5 41 216.5 42 209.0 43 241.0 44 263.0 45 237.5 46 246.0 47 275.0 48 239.5 49 179.0 50 148.0 51 140.0 52 126.5 53 111.0 54 93.5 55 68.0 56 51.0 57 49.0 58 32.0 59 16.0 60 16.5 61 12.0 62 4.0 63 1.5 64 2.5 65 1.5 66 0.5 67 0.0 68 2.0 69 2.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.4 #Duplication Level Percentage of deduplicated Percentage of total 1 67.3524844720497 43.375 2 19.72049689440994 25.4 3 7.608695652173914 14.7 4 2.8338509316770186 7.3 5 1.3198757763975155 4.25 6 0.6987577639751553 2.7 7 0.3105590062111801 1.4000000000000001 8 0.07763975155279502 0.4 9 0.03881987577639751 0.22499999999999998 >10 0.03881987577639751 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGGGCTTGATGTGATGGGAGTCTCAAGGCTTCCAATGAAGGGATCGCCA 10 0.25 No Hit TTCTAATTCATTTCCATTGAGGTTGAGATATTCCAAGCTATTATCCTTTG 9 0.22499999999999998 No Hit GTTGGGATTTGCTACTGCTGGAGGGGGCCAGATTTATGGATGTAAGCTTT 8 0.2 No Hit CTTCAATATATACAGGAATATCTTCACAAATCTCTCAAAGTGATTAATTT 8 0.2 No Hit ATCGGTACTCCTCCCATGTGAGGCGGGAGTTTGGATTTTCCCTTTTCTTC 7 0.17500000000000002 No Hit CCGGTTTTGAACATCTGTAGACGAAGGTGATGTGGGTAGTCCAAGGCGGT 7 0.17500000000000002 No Hit GGCGTATCGGTCTTGATCTTCTTGACACCACTGGCTTCAATCTTGAAAGT 7 0.17500000000000002 No Hit CGAAACCAAAGTCTCCAGGGAGGCTTCCATCAAGCCATGGAGGAGGGGTG 7 0.17500000000000002 No Hit CGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTT 7 0.17500000000000002 No Hit CCTTGGTATAAAATATCTGAGCCAAAGTATTCAGCAATATCATCAAGCAG 7 0.17500000000000002 No Hit AGATACTCTTTAGGACACCTGAAGCTGCATTGACTCGCTCCTTGTTCCAC 7 0.17500000000000002 No Hit GACCGATTTCCGAGGTAAAACGGATGGTTGCCTGACCACACATCCACCAC 7 0.17500000000000002 No Hit AGCTCGTCTGCGTTTTTTAAAGGCTGAGCTGCTGCTGATGCTTTGGTCGT 6 0.15 No Hit ACAACGTGTAACTAAGAAGCATAGCATCATCACATATTGCAATTGTGGCA 6 0.15 No Hit CTCACCTCATCGTCATAAACTCGGGCCCTGAGAGCACCAAAGAAATCGAT 6 0.15 No Hit CGTAGGAGGAGGAAAAAAGTTACAAAATCTGCAATCGATGGCCTCACAAA 6 0.15 No Hit CTCCCTTCCTTGCACATCCCATTGACAAGAACATTATATGTAACCACATC 6 0.15 No Hit CCCCACAGTTCACCCTAACAGGGTGAGAGACACCAATTGCACTACAGAGA 6 0.15 No Hit GGAGATTTGAGCAAGAAAATGCTCCGCTACAAGCCTACGCTGAATCTTTC 6 0.15 No Hit GTGAATTTGATTGAACGTCTCTGGCAGCCAGAAATTGGAATCTGAAAGTG 6 0.15 No Hit CTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACAC 6 0.15 No Hit GTGGTTGGCTAGGTACGCAATATCGCTTACCCCTACCGCAATTGAATGGA 6 0.15 No Hit CCTGTAATAGCCCCTTGGATGAGGAGATCCTTTAATAGGCTTTTGCCCAT 6 0.15 No Hit CCTTGGAAGTGGAGAGGCTGAAACCTTGGCACTGGCACTGACTTTGGCAT 6 0.15 No Hit CTCCAACCTCAATCTTTCCTTTTCCTGGTTCAGGCTCTGCATGTAGATCA 6 0.15 No Hit CATCCAAAGCATTTTAAACAATTGTTCCCTATCAAGTCATCCTCTACCAA 6 0.15 No Hit TGATCAGCAGATGGTTTAATGCCATAACATTTTTCCATCAACTCAAATAT 6 0.15 No Hit ATTTTGACACAGTTTGCGTAGTCTGGTTTGGCTCTACCTTCCATAAGTCC 6 0.15 No Hit CTGACATGCAATTTGTCACCTCTTCTGTTAAATACACTAGACCTTTTCCA 6 0.15 No Hit GGAGCTGACATGAATGCCAAATAATGTAGCAGGGTTGTAAACACTCATTC 6 0.15 No Hit AGCTGCAAGGCAGGCCTGGACATTGAGGCCGAAGAAGAGAACGGCGGAGG 5 0.125 No Hit TGTCTATCGGCCTCACCGAACAAGCCCGCAGGTCGAATGGCGCACGTCTT 5 0.125 No Hit GTCAAACCAATCTCGTTTTTTAAATCATCTCCATCTCCTCTGCGGGGCTT 5 0.125 No Hit TGGCAAAGGTCTCTGGGTCAGCAGAGAGGCCAGCAGTGTCCCAGCCGTAG 5 0.125 No Hit CGGCAGAACAATAAATCTAAAAGCAACATGGGAAGCTGGGATCCCAGTCA 5 0.125 No Hit CTTTGATTTGCTTCTTTGGAGGAGTAGTGTAAACATAAAAGGGTACTTCT 5 0.125 No Hit TCCCGGTGTGCTCTTTCTGAGTAAAAATGTTCTTTAGCCTTGAGAGGAAC 5 0.125 No Hit GGCAAGGTTCCAATCCAGAGAACAAGAAAGAATCATGAAGCGGATTCTCA 5 0.125 No Hit GTCAGCAAGAGTCCTCCCATCTTCAAGCTGCTTCCCAGCAAAAATCAACC 5 0.125 No Hit GGGCTCCGAGCGCCAATGCCTTGAAGACATCAGTTCCACGCCTTACACCA 5 0.125 No Hit CCCCAGGTGTTCAATGCATCGTCATCATTCCTAAGAAAGACCTCTAAAAC 5 0.125 No Hit CGTGAAATGATGCAATCCTATAAAGATCCAACCCCGCCGATTCTTTACCC 5 0.125 No Hit TCCATGTGTATCGGTCAGCAGGAGCAACACTGCCATGAACCATATAATTG 5 0.125 No Hit GACGTCATGTGCAAATAAAGCTAAAACCCTAGCCCTAACCCTAACTCTAA 5 0.125 No Hit AGACACGATGAGCCGTCCATACTTTTTAAGCACACAACCTTGCTTGCTTC 5 0.125 No Hit CCCTTGTCGGTCTGCTTCTTCTCATCGTACTCTGCAACAACCTTGAAGCT 5 0.125 No Hit CAACACACTACAAGGCTCTCTTAAAATCTAGAAAACATCCTTCTTAAGCT 5 0.125 No Hit GCACCCCCAAAGAATGATAAGCTCTCATTATTTTTGAATAACATGGGGTT 5 0.125 No Hit GGCTGATTTTTGCATTCAAGGTCTGCCTAGTCTTCTCTAATCCTTCATCA 5 0.125 No Hit ACCTACGACAATATATATGGAGCTTTTAACCAATCCCCACAAGTTCTTTC 5 0.125 No Hit GCTTCATGGTCAGGATCAATTGTCTGGATGAAAAGTTTCCACTCAGGATA 5 0.125 No Hit CTCCAATGCCTTCTCTACGCCTACGTCTCCTACCTGAACGCCCCAAGCAT 5 0.125 No Hit GTCCCATCTCAGCAATTCTCTGCTGAATAACCTCATAGCGCTCCTCCCTT 5 0.125 No Hit GCGGCAGATGCGAGCAGTTGGCATTGTGACACAAATAGGAGCATACTTCT 5 0.125 No Hit TTACTAAAAACAACTTTTAATTTCATAAATATATTACAATCATACATGGA 5 0.125 No Hit CACCATCATTTTCATCAATTTATTCAGATTTAGGATTATATCTTACCTAA 5 0.125 No Hit CCGGGACATCAACAGTCACCTGTCTATCAAACCGTCCTGGCCTCAACAAG 5 0.125 No Hit GGCCATAAAAATGGGGTTGTATCCGATGCTGAAGTTGAGGTCTTCCAATC 5 0.125 No Hit TGTGCACTGTGAGGGAGATCAGGACGCAGCCCCTGTCTCACTCCCAGGGC 5 0.125 No Hit GTGCGAACTTTAATTCCATCCTCTGTTTTAGTCAACTCTGTCAAATTTGT 5 0.125 No Hit GGTGATTGTTCGGTCCCATTGGTGTCACTAATATGGCCTGCTTCACTACA 5 0.125 No Hit CACGTTGACTGGAACCGAAGCTTCTTGGATATTTTTCAGAAGTTGCTCCT 5 0.125 No Hit TTCGGTCGATCTAGAAACCACCTGCTTCTGGAACAATATATTCTAGTATA 5 0.125 No Hit CTCCAGGAGACAATGTGTTGGTAAGTGCACTCTCCCATGCACCAGTGCCT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0125 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.037500000000000006 0.0 0.0 0.0 0.0 74-75 0.0625 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.15 0.0 0.0 0.0 0.0 80-81 0.15 0.0 0.0 0.0 0.0 82-83 0.22499999999999998 0.0 0.0 0.0 0.0 84-85 0.3375 0.0 0.0 0.0 0.0 86-87 0.425 0.0 0.0 0.0 0.0 88-89 0.44999999999999996 0.0 0.0 0.0 0.0 90-91 0.6 0.0 0.0 0.0 0.0 92-93 0.725 0.0 0.0 0.0 0.0 94-95 0.925 0.0 0.0 0.0 0.0 96-97 1.0625 0.0 0.0 0.0 0.0 98-99 1.3625 0.0 0.0 0.0 0.0 100-101 1.6125 0.0 0.0 0.0 0.0 102-103 1.7125 0.0 0.0 0.0 0.0 104-105 1.85 0.0 0.0 0.0 0.0 106-107 2.1125 0.0 0.0 0.0 0.0 108-109 2.325 0.0 0.0 0.0 0.0 110-111 2.5999999999999996 0.0 0.0 0.0 0.0 112-113 2.75 0.0 0.0 0.0 0.0 114-115 3.0 0.0 0.0 0.0 0.0 116-117 3.175 0.0 0.0 0.0 0.0 118-119 3.55 0.0 0.0 0.0 0.0 120-121 3.9250000000000003 0.0 0.0 0.0 0.0 122-123 4.375 0.0 0.0 0.0 0.0 124-125 5.15 0.0 0.0 0.0 0.0 126-127 5.675 0.0 0.0 0.0 0.0 128-129 6.2875 0.0 0.0 0.0 0.0 130-131 6.5375 0.0 0.0 0.0 0.0 132-133 6.925000000000001 0.0 0.0 0.0 0.0 134-135 7.5625 0.0 0.0 0.0 0.0 136-137 7.875 0.0 0.0 0.0 0.0 138-139 8.55 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGATGCA 10 0.006830828 145.0 4 GATGCAA 10 0.006830828 145.0 5 ATGAAAC 10 0.006830828 145.0 7 GATGAAA 10 0.006830828 145.0 6 GCACCAC 10 0.006830828 145.0 145 ACTGGAT 10 0.006830828 145.0 1 >>END_MODULE SRR13695443 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695443_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.1375 37.0 37.0 37.0 37.0 37.0 2 36.214 37.0 37.0 37.0 37.0 37.0 3 36.198 37.0 37.0 37.0 37.0 37.0 4 36.184 37.0 37.0 37.0 37.0 37.0 5 36.3115 37.0 37.0 37.0 37.0 37.0 6 36.338 37.0 37.0 37.0 37.0 37.0 7 36.2785 37.0 37.0 37.0 37.0 37.0 8 36.2595 37.0 37.0 37.0 37.0 37.0 9 36.1 37.0 37.0 37.0 37.0 37.0 10-14 36.282799999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.2423 37.0 37.0 37.0 37.0 37.0 20-24 36.20465 37.0 37.0 37.0 37.0 37.0 25-29 36.12874999999999 37.0 37.0 37.0 37.0 37.0 30-34 36.11725 37.0 37.0 37.0 37.0 37.0 35-39 36.11265 37.0 37.0 37.0 37.0 37.0 40-44 36.08505 37.0 37.0 37.0 37.0 37.0 45-49 36.126599999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.01955 37.0 37.0 37.0 37.0 37.0 55-59 36.013549999999995 37.0 37.0 37.0 37.0 37.0 60-64 35.97805 37.0 37.0 37.0 37.0 37.0 65-69 35.948699999999995 37.0 37.0 37.0 37.0 37.0 70-74 35.88415 37.0 37.0 37.0 37.0 37.0 75-79 35.90115 37.0 37.0 37.0 37.0 37.0 80-84 35.91435 37.0 37.0 37.0 37.0 37.0 85-89 35.757549999999995 37.0 37.0 37.0 37.0 37.0 90-94 35.775850000000005 37.0 37.0 37.0 37.0 37.0 95-99 35.76035 37.0 37.0 37.0 37.0 37.0 100-104 35.82045000000001 37.0 37.0 37.0 37.0 37.0 105-109 35.75595 37.0 37.0 37.0 37.0 37.0 110-114 35.72605 37.0 37.0 37.0 37.0 37.0 115-119 35.627449999999996 37.0 37.0 37.0 37.0 37.0 120-124 35.56855 37.0 37.0 37.0 37.0 37.0 125-129 35.590250000000005 37.0 37.0 37.0 37.0 37.0 130-134 35.39695 37.0 37.0 37.0 37.0 37.0 135-139 35.43185 37.0 37.0 37.0 34.6 37.0 140-144 35.25825 37.0 37.0 37.0 32.2 37.0 145-149 35.114850000000004 37.0 37.0 37.0 27.4 37.0 150-151 34.89875 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 1.0 13 4.0 14 2.0 15 3.0 16 0.0 17 2.0 18 0.0 19 0.0 20 3.0 21 0.0 22 1.0 23 1.0 24 8.0 25 8.0 26 6.0 27 9.0 28 16.0 29 18.0 30 21.0 31 43.0 32 64.0 33 109.0 34 238.0 35 581.0 36 2682.0 37 179.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.76004016064257 21.285140562248998 14.38253012048193 30.57228915662651 2 28.199999999999996 26.325 28.775000000000002 16.7 3 20.25 28.15 32.125 19.475 4 24.474999999999998 33.375 23.150000000000002 19.0 5 26.125 33.2 22.825 17.849999999999998 6 20.8 40.0 20.849999999999998 18.35 7 20.9 22.45 38.65 18.0 8 21.525 25.575 28.275 24.625 9 20.9 25.25 30.425 23.425 10-14 23.044999999999998 30.080000000000002 26.515 20.36 15-19 23.22 27.58 28.07 21.13 20-24 23.093082578902617 28.364927724703648 27.72970539688891 20.812284299504828 25-29 23.212409306980238 28.576432324243186 27.51563672754566 20.695521641230926 30-34 22.579676789913446 28.088257367288737 29.04888177315255 20.28318406964527 35-39 22.80298104336518 27.90976841894663 27.62466863402191 21.662581903666283 40-44 23.237780779428686 29.306118365100804 26.724698584221322 20.731402271249184 45-49 23.721860930465233 28.189094547273637 27.098549274637318 20.990495247623812 50-54 22.657930275596456 28.449957485119793 27.744710648727057 21.147401590556694 55-59 23.157368026019515 28.211158368776584 27.500625469101823 21.130848136102077 60-64 21.982693942880008 28.214875206322215 28.014805181813635 21.787625668984145 65-69 23.399679935987198 27.28045609121824 27.790558111622328 21.529305861172237 70-74 22.987240430322743 28.261195896922693 27.025268951713787 21.72629472104078 75-79 23.377533149862398 28.32124093069802 27.905929447085313 20.395296472354264 80-84 22.870717679419855 28.127031757939484 27.431857964491122 21.57039259814954 85-89 23.432574430823117 27.960970728046036 27.270452839629723 21.336002001501125 90-94 24.090840878395277 28.162673202941324 26.972137461857837 20.774348456805562 95-99 23.798329415295353 26.86440254088931 27.799729905466915 21.537538138348424 100-104 23.332499374530897 28.261195896922693 27.52564423317488 20.88066049537153 105-109 23.152364273204903 27.710783087315487 28.036027020265198 21.10082561921441 110-114 24.918721552543392 27.314560096033613 27.14450057520132 20.622217776221678 115-119 24.778583937953467 28.06104578433825 26.75006254691018 20.4103077307981 120-124 24.3382536902677 28.04103077307981 27.425569176882664 20.19514635976983 125-129 24.33081502976935 27.91814679541702 27.47786060939611 20.273177565417523 130-134 24.43832874655992 28.506379784838632 27.35551663747811 19.699774831123342 135-139 25.889417062797097 27.440580435326495 26.870152614460846 19.79984988741556 140-144 26.014104936727854 27.949782423848347 27.199519831941178 18.836592807482617 145-149 27.185389041781338 27.78583937953465 26.184638478859146 18.84413309982487 150-151 26.857643232424316 27.47060295221416 25.369026770077557 20.302727045283962 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 0.5 8 0.5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.5 20 0.5 21 0.0 22 0.5 23 0.5 24 0.0 25 4.0 26 5.0 27 2.5 28 9.0 29 15.0 30 16.0 31 19.0 32 21.5 33 28.0 34 42.0 35 58.5 36 78.5 37 88.5 38 124.5 39 169.5 40 193.5 41 238.0 42 274.0 43 272.5 44 266.0 45 278.5 46 287.5 47 292.0 48 250.0 49 190.0 50 162.0 51 137.5 52 109.5 53 82.0 54 62.0 55 53.5 56 50.0 57 35.5 58 24.5 59 19.0 60 11.5 61 4.5 62 2.5 63 2.0 64 1.5 65 1.5 66 1.0 67 0.5 68 1.0 69 2.0 70 2.0 71 1.0 72 0.5 73 0.5 74 0.0 75 0.0 76 0.0 77 0.5 78 0.5 79 0.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.034999999999999996 25-29 0.075 30-34 0.065 35-39 0.034999999999999996 40-44 0.055 45-49 0.05 50-54 0.034999999999999996 55-59 0.075 60-64 0.034999999999999996 65-69 0.02 70-74 0.075 75-79 0.075 80-84 0.025 85-89 0.075 90-94 0.045 95-99 0.034999999999999996 100-104 0.075 105-109 0.075 110-114 0.034999999999999996 115-119 0.075 120-124 0.075 125-129 0.065 130-134 0.075 135-139 0.075 140-144 0.034999999999999996 145-149 0.075 150-151 0.075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.125 #Duplication Level Percentage of deduplicated Percentage of total 1 68.56046065259117 44.65 2 18.963531669865645 24.7 3 7.370441458733206 14.399999999999999 4 2.91746641074856 7.6 5 0.998080614203455 3.25 6 0.6142034548944338 2.4 7 0.345489443378119 1.575 8 0.07677543186180423 0.4 9 0.038387715930902115 0.22499999999999998 >10 0.11516314779270634 0.8 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAAT 11 0.27499999999999997 No Hit AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 11 0.27499999999999997 No Hit ACGCTCTATTCTCCAAGCTCTTTTAACACAGGAAGAAGAAGGGGCCATCA 10 0.25 No Hit CTTCGGGTCATGGACTTGTCCAACAGCAGCTTTAGTGGTTCTATGCCACT 9 0.22499999999999998 No Hit TAACTCTGGTGGTACAGGTGATAGCTCTAAACAGTTCTCAAGCAGCTGAA 8 0.2 No Hit GTTAAAATCAGGAAATTTTCTGGAGATGGGAAGAAATGGCGGATGTGAAC 8 0.2 No Hit CGCTTCGGAAGAAAATCTTTTGGCCACAAAAATGGCTTCTGTTTGTGCTT 7 0.17500000000000002 No Hit GGAACTAATTAAAGGACGTGATGTAGCTGTTGAACTCAAAGTTGAAGATG 7 0.17500000000000002 No Hit TGAAGACTGAGAAGTTCTTGACGGAGGATTTCATCCCCCAACTCATGTTC 7 0.17500000000000002 No Hit CTTTCCTTGACGAGCTATCTGATTTGACTAGGTTCAGGACACTAGAGCTG 7 0.17500000000000002 No Hit GCAGAAGCTAAGTCAGCAATGGCAGCCTCAGTAATGGCTTCATTGAGCCT 7 0.17500000000000002 No Hit CAAAAAAAATCTTAACTGCACCCGCTCATCCAGCAATGGCAGCAGCAACA 7 0.17500000000000002 No Hit CTTACAATTTATTGCAATTGTTGGCCTCGGTCAGACTATTTATCGGCGGG 7 0.17500000000000002 No Hit CAGACACAAGAAATGGCACTTAGTCTGACTAACACTTTCCTCCAAACCAA 7 0.17500000000000002 No Hit AGCAGGACAGTTGTTTCAGTGTCTGCTGTTTCTTCTTCCTCTTCAACATC 7 0.17500000000000002 No Hit AGAAGATATCACCAATTGAAGTGGATGCAGTACTTTTGTCTCATCCTGAC 6 0.15 No Hit AGAAATTCAGACATCCAAAAGAAGGAAGGTGGTTGAGAAGACTGTTGTTA 6 0.15 No Hit TGGGAGGAAACAAAGATATGTGTACGGGACAATTCTGGACAGCATTGCAA 6 0.15 No Hit CAGATGCTCTAGATGCTGCTGGCAACACCACTGCTGCAATTGGCAAGGGT 6 0.15 No Hit GTAAAGAACTTTTGGAATGGGGTTCTAGGGTCTCTGCTGCCAATAGCCGT 6 0.15 No Hit CCACTGTTGTGGTATTATTACTTGCCTCAACATTTACTTTCATATAAAAT 6 0.15 No Hit GTTGATGAGGTTGTTCGTGGGAGAGCCAGTGTGGACAGCTTATAATCGCC 6 0.15 No Hit GGTTGCTTGAGAGGCCATCGCTGTTTGGGGCATTGGTTCAGGAGTATGCC 6 0.15 No Hit CCCAGATGTTGTTACATATAATACGATCTTGCGTACCTTGTGTGATAGTG 6 0.15 No Hit CTTTGATTGATATGTATTCAAAGTGTGGGAGTGTTGGAGATGCGTATAGA 6 0.15 No Hit ATAAGCAAAGTTTCTCTTTCCTGGCCACTAATAAAGTTGAATAGGATCCA 6 0.15 No Hit AAAGTTGCTCTCTTCCAATGAGATGGACTCGGAAGCCATGGGTGTAAACA 6 0.15 No Hit GTCAACAACCAGATGGTTAATGCTACCCTCATGAACATTGCTGACAACCC 6 0.15 No Hit AGCAGAGATTATGGTCAAGAGCATGGCAGTCAATAACTTTTATGTTGGGG 6 0.15 No Hit ATAGGAGGAGAGCATAACCATTTTAGTCACATATATTTCCAAGATGAAGG 6 0.15 No Hit TAAAACCAGCTAGATTCCCTACTCCTTCCGATTAATCGCCTTCAGCACAC 6 0.15 No Hit TGCAGATCTTTGTGAAGACCCTCACAGGAAAGACCATCACCTTGGAGGTG 5 0.125 No Hit AGTAACTAAGGTTGATTACATGACATTGAGACATGGATTTATCACGGCAC 5 0.125 No Hit TATATTTCCAAGATGAAGGCCTTTCTTATCGTATGCTTTCTCTTAGCTAC 5 0.125 No Hit CATGGATCCCAGGCAAGGTTCTTATTGGCAAAATTGAACCCATCAGCTAC 5 0.125 No Hit CTGGAGTTCTTTCATCTTCCTTGAATAATGAATGCCAAGGTTTAACGTGT 5 0.125 No Hit AGGAGATAAGCGTGCCAAGGAATATGTAAGCCTCCTGAAATCAAATGATC 5 0.125 No Hit ATATGGTGTCTATTCATGCATCTGGAATTGCTGGAAGTGCTATGCATGAG 5 0.125 No Hit AAGAGAGGGTACATAGAGGGTTCTGCTTATAAGGCTGATCCTAACAGTGA 5 0.125 No Hit TTCATCACCGACTCAAGTGTCCAATGATGATGAGAGGGATGATTTTTATG 5 0.125 No Hit CCCTAGTCACTCTACCCTTCGCATTACTTCCTCAATCAACCACTGCTGTT 5 0.125 No Hit TGTCAGGGTTGCTTCAACATAACAACTGTTTTTAGTCACTCGCAAACTGT 5 0.125 No Hit ATTTAATGTAAGGTCAGTCCAAAATAAAATTGTCTACCAAATTTTGGGTG 5 0.125 No Hit AACAAGAAAGCCATGTCGATCCAGTGCAAGGTGTGTATGCAGACATTCAT 5 0.125 No Hit CCTGAAAGTTTGCACATGTTCTCCTTCCTATTTGATGATTTGGGTGTTCC 5 0.125 No Hit GTTACTATCTAATGCTGGGGTTAAACTGTATGAAGGAGAGGGAAAGGTTG 5 0.125 No Hit GTGGGAAGGCAAAGAGGAACTGGAATTGGCGGAGGAAATGATGAAAGAGA 5 0.125 No Hit TTTGCAACTAATTCTTTCTCATTTCATATTCTCGAAGGAGAGAATTTTTC 5 0.125 No Hit CCAGCCTGGAGCCACAGCTCCTCCAAGAGATAAAGTTGTCATCTTGGGTG 5 0.125 No Hit CGTATATTTTTGCTCCTCAGATAAGAAGGTCCCTGCTAAATGAAGATGAA 5 0.125 No Hit TCAAGTCCTAAAACCAACAGCAACTGTAAGATACAAACACACAACAGAGT 5 0.125 No Hit AGCAGCCGCAACCACATGGGGCACCAGCAACCTCTTCTACAGGGATGTTG 5 0.125 No Hit CGCTGATGATCCTGATGCATTTGCAGAGTTGAAGGTGAAGGAACTCAAGA 5 0.125 No Hit TGATCGTACACTGAGCTGGAAGGACGTGGAGTGGCTTCAGACAATCACCA 5 0.125 No Hit AGAGTCTCTTACTTTGCGGTATAACAATCTATGTCGGGAAGCCATAAAAT 5 0.125 No Hit GGATAATGCTGAGGAATCTGATGCAAATGCCAGTGAGACCTCAGAGCCAC 5 0.125 No Hit TTTATGGATGTATCAACCCAACCTCAATGTGTTGAGAGAAAAGATACAAC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0125 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.037500000000000006 0.0 0.0 0.0 0.0 74-75 0.0625 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.15 0.0 0.0 0.0 0.0 80-81 0.15 0.0 0.0 0.0 0.0 82-83 0.22499999999999998 0.0 0.0 0.0 0.0 84-85 0.3375 0.0 0.0 0.0 0.0 86-87 0.45 0.0 0.0 0.0 0.0 88-89 0.475 0.0 0.0 0.0 0.0 90-91 0.6375 0.0 0.0 0.0 0.0 92-93 0.775 0.0 0.0 0.0 0.0 94-95 0.975 0.0 0.0 0.0 0.0 96-97 1.1125 0.0 0.0 0.0 0.0 98-99 1.4125 0.0 0.0 0.0 0.0 100-101 1.6625 0.0 0.0 0.0 0.0 102-103 1.7625000000000002 0.0 0.0 0.0 0.0 104-105 1.9 0.0 0.0 0.0 0.0 106-107 2.1624999999999996 0.0 0.0 0.0 0.0 108-109 2.375 0.0 0.0 0.0 0.0 110-111 2.6625 0.0 0.0 0.0 0.0 112-113 2.825 0.0 0.0 0.0 0.0 114-115 3.075 0.0 0.0 0.0 0.0 116-117 3.25 0.0 0.0 0.0 0.0 118-119 3.625 0.0 0.0 0.0 0.0 120-121 4.0 0.0 0.0 0.0 0.0 122-123 4.4375 0.0 0.0 0.0 0.0 124-125 5.225 0.0 0.0 0.0 0.0 126-127 5.762499999999999 0.0 0.0 0.0 0.0 128-129 6.3875 0.0 0.0 0.0 0.0 130-131 6.637499999999999 0.0 0.0 0.0 0.0 132-133 7.0125 0.0 0.0 0.0 0.0 134-135 7.65 0.0 0.0 0.0 0.0 136-137 7.975 0.0 0.0 0.0 0.0 138-139 8.65 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGGTC 10 0.006830828 145.0 7 GCGACCG 10 0.006830828 145.0 4 GGCGACC 10 0.006830828 145.0 3 GACCGGT 10 0.006830828 145.0 6 GCTTGGT 10 0.006830828 145.0 6 CATGGGA 10 0.006830828 145.0 145 CGGTCTT 10 0.006830828 145.0 9 GGGCGAC 10 0.006830828 145.0 2 CGACCGG 10 0.006830828 145.0 5 CCGGTCT 10 0.006830828 145.0 8 >>END_MODULE Read 1016080 spots for SRR13695443.sra Written 1016080 spots for SRR13695443.sra Read 1016080 spots for SRR13695443.sra Written 1016080 spots for SRR13695443.sra Read 1016080 spots for SRR13695443.sra Written 1016080 spots for SRR13695443.sra Read 1016080 spots for SRR13695443.sra Written 1016080 spots for SRR13695443.sra Read 1016080 spots for SRR13695443.sra Written 1016080 spots for SRR13695443.sra Read 1016080 spots for SRR13695443.sra Written 1016080 spots for SRR13695443.sra Read 1016080 spots for SRR13695443.sra Written 1016080 spots for SRR13695443.sra Read 1016080 spots for SRR13695443.sra Written 1016080 spots for SRR13695443.sra Read 1016080 spots for SRR13695443.sra Written 1016080 spots for SRR13695443.sra Read 1016080 spots for SRR13695443.sra Written 1016080 spots for SRR13695443.sra Read 1016080 spots for SRR13695443.sra Written 1016080 spots for SRR13695443.sra Read 1016080 spots for SRR13695443.sra Written 1016080 spots for SRR13695443.sra Read 1016080 spots for SRR13695443.sra Written 1016080 spots for SRR13695443.sra Read 1016080 spots for SRR13695443.sra Written 1016080 spots for SRR13695443.sra Read 1016080 spots for SRR13695443.sra Written 1016080 spots for SRR13695443.sra Read 1016080 spots for SRR13695443.sra Written 1016080 spots for SRR13695443.sra Read 1016080 spots for SRR13695443.sra Written 1016080 spots for SRR13695443.sra Read 1016080 spots for SRR13695443.sra Written 1016080 spots for SRR13695443.sra Read 1016090 spots for SRR13695443.sra Written 1016090 spots for SRR13695443.sra Read 1016080 spots for SRR13695443.sra Written 1016080 spots for SRR13695443.sra SRR ids: ['SRR13695443.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_ui8hpaua SRR13695443.sra spots: 20321610 blocks: [[1, 1016080], [1016081, 2032160], [2032161, 3048240], [3048241, 4064320], [4064321, 5080400], [5080401, 6096480], [6096481, 7112560], [7112561, 8128640], [8128641, 9144720], [9144721, 10160800], [10160801, 11176880], [11176881, 12192960], [12192961, 13209040], [13209041, 14225120], [14225121, 15241200], [15241201, 16257280], [16257281, 17273360], [17273361, 18289440], [18289441, 19305520], [19305521, 20321610]] SRR13695443 file size 6884471 SRR13695443 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695443 SRR13695443_1.fastq SRR13695443_2.fastq Input file: SRR13695443_1.fastq Paired file: SRR13695443_2.fastq trimmed: SRR13695443-trimmed-pair1.fastq, SRR13695443-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 02:52:43 2025 >> started Wed Feb 12 02:53:06 2025 >> done (22.801s) 20321610 read pairs processed; of these: 149 ( 0.00%) short read pairs filtered out after trimming by size control 10357 ( 0.05%) empty read pairs filtered out after trimming by size control 20311104 (99.95%) read pairs available; of these: 2453003 (12.08%) trimmed read pairs available after processing 17858101 (87.92%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 2 0.00% 20 2 0.00% 21 1 0.00% 22 2 0.00% 23 3 0.00% 24 5 0.00% 25 2 0.00% 26 2 0.00% 27 4 0.00% 28 1 0.00% 29 3 0.00% 30 5 0.00% 31 9 0.00% 32 8 0.00% 33 6 0.00% 34 8 0.00% 35 5 0.00% 36 12 0.00% 37 12 0.00% 38 7 0.00% 39 12 0.00% 40 17 0.00% 41 22 0.00% 42 30 0.00% 43 23 0.00% 44 26 0.00% 45 19 0.00% 46 27 0.00% 47 37 0.00% 48 58 0.00% 49 58 0.00% 50 96 0.00% 51 74 0.00% 52 99 0.00% 53 105 0.00% 54 95 0.00% 55 100 0.00% 56 133 0.00% 57 163 0.00% 58 196 0.00% 59 262 0.00% 60 337 0.00% 61 342 0.00% 62 447 0.00% 63 398 0.00% 64 475 0.00% 65 565 0.00% 66 613 0.00% 67 678 0.00% 68 706 0.00% 69 811 0.00% 70 1031 0.01% 71 1150 0.01% 72 1403 0.01% 73 1643 0.01% 74 1874 0.01% 75 2074 0.01% 76 2334 0.01% 77 2511 0.01% 78 2851 0.01% 79 3159 0.02% 80 3509 0.02% 81 3942 0.02% 82 4460 0.02% 83 4888 0.02% 84 5573 0.03% 85 6110 0.03% 86 6645 0.03% 87 7125 0.04% 88 7941 0.04% 89 8537 0.04% 90 8872 0.04% 91 9801 0.05% 92 10685 0.05% 93 11743 0.06% 94 12488 0.06% 95 13640 0.07% 96 14601 0.07% 97 15218 0.07% 98 15921 0.08% 99 16843 0.08% 100 17492 0.09% 101 18631 0.09% 102 19343 0.10% 103 20403 0.10% 104 21726 0.11% 105 23339 0.11% 106 24139 0.12% 107 25195 0.12% 108 25649 0.13% 109 26967 0.13% 110 27639 0.14% 111 28511 0.14% 112 29672 0.15% 113 30397 0.15% 114 32017 0.16% 115 33418 0.16% 116 34705 0.17% 117 36179 0.18% 118 36930 0.18% 119 38091 0.19% 120 39580 0.19% 121 39917 0.20% 122 40837 0.20% 123 42103 0.21% 124 42941 0.21% 125 44213 0.22% 126 45502 0.22% 127 46650 0.23% 128 48391 0.24% 129 48068 0.24% 130 49568 0.24% 131 50392 0.25% 132 51642 0.25% 133 52258 0.26% 134 53355 0.26% 135 54383 0.27% 136 56112 0.28% 137 57159 0.28% 138 57606 0.28% 139 59759 0.29% 140 60117 0.30% 141 60899 0.30% 142 61382 0.30% 143 61594 0.30% 144 63090 0.31% 145 63800 0.31% 146 65129 0.32% 147 65390 0.32% 148 67607 0.33% 149 68174 0.34% 150 69246 0.34% 151 17858101 87.92% 20311104 reads passed initial QC criterion=sequence-density sequence-density=0.46 sequence-density-rank=1 fanout-score=1.98 fanout-score-rank=22 prefix-density=0.46 prefix-fanout=2.0 sequence=GTGTTGTCGAATCC criterion=fanout-score sequence-density=0.01 sequence-density-rank=25 fanout-score=317.40 fanout-score-rank=1 prefix-density=0.28 prefix-fanout=15.2 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGTTGTCGCAGGTG criterion=sequence-density sequence-density=0.98 sequence-density-rank=1 fanout-score=1.95 fanout-score-rank=30 prefix-density=0.98 prefix-fanout=2.0 sequence=CTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAGTGAACAAACAAAAGTGTCCGTGAGTGTCTTTATCTTTGTGTCTTCTTCA criterion=fanout-score sequence-density=0.14 sequence-density-rank=26 fanout-score=27.42 fanout-score-rank=1 prefix-density=0.36 prefix-fanout=11.0 sequence=AAAGAAAAGAAAA SRR13695443 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 02:53:49 Started mapping on | Feb 12 02:53:50 Finished on | Feb 12 02:55:54 Mapping speed, Million of reads per hour | 589.68 Number of input reads | 20311104 Average input read length | 296 UNIQUE READS: Uniquely mapped reads number | 19153895 Uniquely mapped reads % | 94.30% Average mapped length | 294.63 Number of splices: Total | 18998134 Number of splices: Annotated (sjdb) | 18603914 Number of splices: GT/AG | 18609073 Number of splices: GC/AG | 311273 Number of splices: AT/AC | 12077 Number of splices: Non-canonical | 65711 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.03% Deletion average length | 2.87 Insertion rate per base | 0.02% Insertion average length | 2.42 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 459929 % of reads mapped to multiple loci | 2.26% Number of reads mapped to too many loci | 37550 % of reads mapped to too many loci | 0.18% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.15% % of reads unmapped: other | 0.10% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 697523 697523 697523 N_multimapping 459929 459929 459929 N_noFeature 612905 18667627 888835 N_ambiguous 329685 2134 117966 UnstrandedReadsAssigned:18211305 PositiveStrandReadsAssigned:484134 NegativeStrandReadsAssigned:18147094 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695443 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695443-trimmed-pair1.fastq SRR13695443-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 20,311,104 reads, 18,128,619 reads pseudoaligned [quant] estimated average fragment length: 247.755 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,065 rounds 52401 SRR13695443.ke.tsv 34699 SRR13695443.se.tsv 87100 total ==> SRR13695443.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1771.25 541 14.0488 Potri.005G024800.1.v4.1 1035 788.245 340 19.8398 Potri.004G059700.1.v4.1 961 714.349 8 0.515109 Potri.007G009000.2.v4.1 1416 1169.25 0 0 Potri.003G141000.2.v4.1 2943 2696.25 784.301 13.3796 Potri.016G087400.1.v4.1 270 86.4197 1128 600.366 Potri.015G069301.1.v4.1 564 326.417 0 0 Potri.010G195200.1.v4.1 1773 1526.25 55 1.65752 Potri.012G127500.1.v4.1 977 730.295 60 3.77896 ==> SRR13695443.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 265 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 258 Potri.001G212900.v4.1 12 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 7 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 12 SRR13695443 completed mapping pipeline successfully