Starting /dee2/code/volunteer_pipeline.sh SRR13695444 current disk space = 3048840421376 free memory = 1509507772 SRR13695444 SRAfilesize 9ed6f0846823b364955aa3cc360416bb SRR13695444.sra SRR13695444.sra file validated SRR13695444 is paired end SRR13695444 is conventional basespace SRR13695444 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695444_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5105 37.0 37.0 37.0 37.0 37.0 2 36.2965 37.0 37.0 37.0 37.0 37.0 3 36.4985 37.0 37.0 37.0 37.0 37.0 4 36.5015 37.0 37.0 37.0 37.0 37.0 5 36.5965 37.0 37.0 37.0 37.0 37.0 6 36.58 37.0 37.0 37.0 37.0 37.0 7 36.5155 37.0 37.0 37.0 37.0 37.0 8 36.5955 37.0 37.0 37.0 37.0 37.0 9 36.535 37.0 37.0 37.0 37.0 37.0 10-14 36.576299999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.522800000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.521300000000004 37.0 37.0 37.0 37.0 37.0 25-29 36.4749 37.0 37.0 37.0 37.0 37.0 30-34 36.4415 37.0 37.0 37.0 37.0 37.0 35-39 36.4128 37.0 37.0 37.0 37.0 37.0 40-44 36.4485 37.0 37.0 37.0 37.0 37.0 45-49 36.3663 37.0 37.0 37.0 37.0 37.0 50-54 36.297700000000006 37.0 37.0 37.0 37.0 37.0 55-59 36.3104 37.0 37.0 37.0 37.0 37.0 60-64 36.3264 37.0 37.0 37.0 37.0 37.0 65-69 36.215199999999996 37.0 37.0 37.0 37.0 37.0 70-74 36.25450000000001 37.0 37.0 37.0 37.0 37.0 75-79 36.2255 37.0 37.0 37.0 37.0 37.0 80-84 36.1542 37.0 37.0 37.0 37.0 37.0 85-89 36.1717 37.0 37.0 37.0 37.0 37.0 90-94 36.142399999999995 37.0 37.0 37.0 37.0 37.0 95-99 36.0803 37.0 37.0 37.0 37.0 37.0 100-104 36.120999999999995 37.0 37.0 37.0 37.0 37.0 105-109 36.0649 37.0 37.0 37.0 37.0 37.0 110-114 36.0101 37.0 37.0 37.0 37.0 37.0 115-119 36.0188 37.0 37.0 37.0 37.0 37.0 120-124 35.9522 37.0 37.0 37.0 37.0 37.0 125-129 35.9476 37.0 37.0 37.0 37.0 37.0 130-134 35.8923 37.0 37.0 37.0 37.0 37.0 135-139 35.8562 37.0 37.0 37.0 37.0 37.0 140-144 35.7084 37.0 37.0 37.0 37.0 37.0 145-149 35.5122 37.0 37.0 37.0 37.0 37.0 150-151 35.483999999999995 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 19 1.0 20 0.0 21 0.0 22 2.0 23 3.0 24 3.0 25 2.0 26 5.0 27 8.0 28 9.0 29 19.0 30 25.0 31 39.0 32 53.0 33 80.0 34 135.0 35 354.0 36 2933.0 37 329.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.875 11.4 7.35 43.375 2 18.790767686904164 14.174611138986453 40.466633216256895 26.567987957852484 3 16.975 18.925 25.5 38.6 4 21.8 25.45 23.45 29.299999999999997 5 22.625 30.25 24.65 22.475 6 20.8 36.125 23.75 19.325 7 16.525000000000002 26.674999999999997 40.625 16.175 8 16.975 27.525 32.525 22.975 9 17.95 24.625 35.5 21.925 10-14 19.49 30.255 27.665 22.59 15-19 20.31 28.410000000000004 28.055000000000003 23.225 20-24 19.985 27.965 27.465 24.585 25-29 19.485 28.849999999999998 28.025 23.64 30-34 20.695 28.22 27.825 23.26 35-39 19.845 28.23 28.444999999999997 23.48 40-44 20.03 28.754999999999995 28.050000000000004 23.165 45-49 19.99 28.7 27.42 23.89 50-54 20.424999999999997 28.685 28.07 22.82 55-59 20.0 28.43 28.105000000000004 23.465 60-64 20.330000000000002 28.595 27.250000000000004 23.825 65-69 20.29 28.54 27.955000000000002 23.215 70-74 20.225 28.244999999999997 28.535 22.994999999999997 75-79 19.79 28.92 27.665 23.625 80-84 20.830000000000002 28.299999999999997 27.735 23.135 85-89 20.880000000000003 28.605000000000004 27.515 23.0 90-94 20.5 28.360000000000003 27.57 23.57 95-99 20.01 28.299999999999997 27.76 23.93 100-104 20.805 28.48 27.345000000000002 23.369999999999997 105-109 20.474999999999998 28.975 26.955000000000002 23.595 110-114 20.105 28.125 28.03 23.74 115-119 20.935000000000002 29.445 26.555 23.064999999999998 120-124 20.135 28.585 27.355 23.925 125-129 20.52 28.449999999999996 27.584999999999997 23.445 130-134 21.58 29.205 26.21 23.005 135-139 20.61 28.99 26.545 23.855 140-144 20.830000000000002 28.405 26.900000000000002 23.865 145-149 21.52 29.115000000000002 25.645 23.72 150-151 19.35 28.925 27.35 24.375 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 2.0 16 1.5 17 0.0 18 1.0 19 1.5 20 0.5 21 0.5 22 1.5 23 2.5 24 4.5 25 3.0 26 5.5 27 10.5 28 10.0 29 12.0 30 25.0 31 34.5 32 36.5 33 42.5 34 59.0 35 74.0 36 90.0 37 111.0 38 132.0 39 158.0 40 185.0 41 207.0 42 233.0 43 241.0 44 254.5 45 278.0 46 262.0 47 254.0 48 236.0 49 211.5 50 181.0 51 142.0 52 106.0 53 94.5 54 86.5 55 57.5 56 43.0 57 25.5 58 20.5 59 18.5 60 10.0 61 8.5 62 7.0 63 4.5 64 4.0 65 1.5 66 3.5 67 3.5 68 0.5 69 0.5 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.35000000000000003 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.475 #Duplication Level Percentage of deduplicated Percentage of total 1 70.32234160800297 47.449999999999996 2 18.45127825120415 24.9 3 7.00259355316784 14.174999999999999 4 2.5194516487587992 6.800000000000001 5 0.9633197480548351 3.25 6 0.444609114486847 1.7999999999999998 7 0.1852537977028529 0.8750000000000001 8 0.037050759540570584 0.2 9 0.0 0.0 >10 0.07410151908114117 0.5499999999999999 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGCCATGGGTTTGCAGACTGGTTCATTGCGTTGAGGTTCAGGGTTGCTT 11 0.27499999999999997 No Hit GGGTTCAATCATAAGTGTTCCAGGAACTGGCCATGACATTGTAGGTGCAT 11 0.27499999999999997 No Hit CTGATCTCAGCGAAGTGTAGACTCACAGTGTAATTCCCATTTTCCAAACA 8 0.2 No Hit GTCTTCTCTAACGAATTGTGCCATCCTCACCACTTTATAATCATCACCAA 7 0.17500000000000002 No Hit ATCGATATCCATTGGTTCAGTCATATTTTCATCATCAATTGTTTTATTTT 7 0.17500000000000002 No Hit CTCCAAGTCAGAAATATCACCTTGATAACGCTTCAGGAAAATAAGGTGGC 7 0.17500000000000002 No Hit GTCATCCTTTTTCTGCTCCACCAACCCGTTTTCATCACCGGGTCTAATCG 7 0.17500000000000002 No Hit CAGTGACCAACTTCTCCTTCAACGAAGCCTTGACTTGAAACCTACCACTG 7 0.17500000000000002 No Hit CTCCTTGTTCCTCACAAAGTGAGTCTGGTTGTAGTCAGTCTTGAATTCTT 6 0.15 No Hit GTTTTAACAACGCGGCATTTAAGAGAAGTGGAGTGTTGAGTCGTGGAGTT 6 0.15 No Hit GCCACTTGTTCCCCTGTACATTGAAGTTTACTTGTTTACATCGATACATA 6 0.15 No Hit CATCCTTTAGCCTGTTCTTCTGCTTTAAATAGGGAATTTTGTCCTGCATC 6 0.15 No Hit AGGAGTGCTCTTAATCTTTGCTGCATGGTCGAGCTGAAAAGGGAAAAGGG 6 0.15 No Hit GTTCGCGTTGATCACAACATGAGTTGCATCGGAGATCATTCTGATAAAGA 6 0.15 No Hit CCAGTAGAAATCTCATCCATAAACAATGCCCTTGATGGTCCAACCAGCAT 6 0.15 No Hit TTTCAGTAACCTCACACTTCTAGTTCTTTTTATTTCAAAGAAATCGAAAT 6 0.15 No Hit TTCCAAAACAAACTGAGGAATCTGAGTGGTTTTACCACTACCAGTCTCGC 6 0.15 No Hit CCGGGCTTGTGAGACCAACCTGAGCATTCATGTTAGCCCCATCCATGTAT 6 0.15 No Hit AGAGGGACAGGATTCCCAGTATCATCAATCACTTCAGCATTGAAACTCTT 6 0.15 No Hit AACGCTACCAATTGTCAACATGGGTTCGATAGTGAAGGTCTGATTCAACA 6 0.15 No Hit AGGAAGTGTTTGGTGGGGGAATGTGTGGTGGAAGACGATGGAGAGTGGAG 5 0.125 No Hit ATCAGATATAGTAGTGGAAGCAGCGGAATCGACAGCAACGGCGGCATCGG 5 0.125 No Hit CACTGAGCACTAAATTGGGACTTCAGCTTGGCTTTTCTGAACCCTCTCCC 5 0.125 No Hit GATATCTCTTATCTTTAACCTCCATTTCCGACGATATCATCACCATTATC 5 0.125 No Hit CTCACCTTCCTGACACGGCAGCACTCCTGATGCCCATCCTCGTAAAAGGA 5 0.125 No Hit GACCTACTTTCATGTGCAAGAATTTTATTCCAGCAATTCCTCGCCCGCCC 5 0.125 No Hit GTAGAGATTGTTCAAGTTCCCAGCAAGCCATTACTGCCTCCATAGCATGA 5 0.125 No Hit CTCCAATGGCAATGCCTCATTTTTCTTCTTTTCGGTTTTAGGAGTTTCAG 5 0.125 No Hit CACCCATCCACCGTAATTAACATCATGTATCTTGTTCCATCAGCCTTCCA 5 0.125 No Hit GTTATAGTGAATCAGCAGTGCATATTTGCCCCAAAAGTACCATCGACAGG 5 0.125 No Hit GTGATGAAACTTTTTGCAGATAGAAGCTTTTCTAAAACTCATCATCTTGA 5 0.125 No Hit GACAGTGTATTGGGTAGTTCCACCAAGCCTCCCAGCTCCGGCATCAAGAT 5 0.125 No Hit CCCGAGTGGGTCAAACCCATTATCACCTGGAAGGCTGCCATTTAGATAAG 5 0.125 No Hit TCCTCTCTTGTCTATGCTACAGTATTGCAAGATTTTACCATCAACCATCT 5 0.125 No Hit CTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCG 5 0.125 No Hit CTTTGCTTCTGGTGCTGCCTCAGTGATTGCTGGAGTTGTAGTAGCTGTTT 5 0.125 No Hit CGCTCTTCGTCTCTCCACTGTACATCCAGTCATAGACAGTGGGTGTGTTA 5 0.125 No Hit GGCAGATATGTCTTTGGAAAAGCTAACAGTTTCCTTTAACTTGTAAAAAC 5 0.125 No Hit CCACGGTGTAAGAGAATATAACCATCTAACTTGTCACACAAGCTGGCTAA 5 0.125 No Hit CTCCAGGAGCCCGGAGAAAATCAGCACATGTGAGGCCAGAGATATCATGG 5 0.125 No Hit CTTTATTGAAGTCTTCGCTGCAGCGTCTACGCGATCAGCATACATAGTTA 5 0.125 No Hit ATATTGCTGCCTTAGGCTACCCAAGTTGTCTAGAGCTCCCTCAAACAAGC 5 0.125 No Hit GGACAAGAGACAAATAAAAGGACTGGACAAGATAAGCAAAGGTCCGAAAA 5 0.125 No Hit GCTAACAGCTCCATCACCATCTGCATCTATGGAAGCAATAATGCCTTGGC 5 0.125 No Hit CTCTTCTTGACACCAGACTTGGCTACCTTAAGACTCCTGTGCACAGCACT 5 0.125 No Hit TTCGCAAGTAATACAGCCGCCTTGTCAAAACAAGCCACATATAGTGACAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0125 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.1125 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.275 0.0 0.0 0.0 0.0 84-85 0.4375 0.0 0.0 0.0 0.0 86-87 0.5 0.0 0.0 0.0 0.0 88-89 0.5625 0.0 0.0 0.0 0.0 90-91 0.6625 0.0 0.0 0.0 0.0 92-93 0.8875 0.0 0.0 0.0 0.0 94-95 1.0375 0.0 0.0 0.0 0.0 96-97 1.1875 0.0 0.0 0.0 0.0 98-99 1.25 0.0 0.0 0.0 0.0 100-101 1.5750000000000002 0.0 0.0 0.0 0.0 102-103 1.8875 0.0 0.0 0.0 0.0 104-105 2.45 0.0 0.0 0.0 0.0 106-107 2.7125 0.0 0.0 0.0 0.0 108-109 2.9749999999999996 0.0 0.0 0.0 0.0 110-111 3.3 0.0 0.0 0.0 0.0 112-113 3.5625 0.0 0.0 0.0 0.0 114-115 3.8499999999999996 0.0 0.0 0.0 0.0 116-117 4.325 0.0 0.0 0.0 0.0 118-119 4.7625 0.0 0.0 0.0 0.0 120-121 5.3 0.0 0.0 0.0 0.0 122-123 5.6 0.0 0.0 0.0 0.0 124-125 6.074999999999999 0.0 0.0 0.0 0.0 126-127 6.8125 0.0 0.0 0.0 0.0 128-129 7.3 0.0 0.0 0.0 0.0 130-131 7.800000000000001 0.0 0.0 0.0 0.0 132-133 8.2625 0.0 0.0 0.0 0.0 134-135 8.8625 0.0 0.0 0.0 0.0 136-137 9.5375 0.0 0.0 0.0 0.0 138-139 10.2 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCGCAT 10 0.006830828 145.0 1 CAATTCT 10 0.006830828 145.0 9 CCGTACA 10 0.006830828 145.0 145 ACGAGGC 10 0.006830828 145.0 9 TCGCATG 10 0.006830828 145.0 2 GCATGAC 10 0.006830828 145.0 4 CGCATGA 10 0.006830828 145.0 3 >>END_MODULE SRR13695444 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695444_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.10475 37.0 37.0 37.0 37.0 37.0 2 36.082 37.0 37.0 37.0 37.0 37.0 3 36.062 37.0 37.0 37.0 37.0 37.0 4 36.1365 37.0 37.0 37.0 37.0 37.0 5 36.152 37.0 37.0 37.0 37.0 37.0 6 36.1025 37.0 37.0 37.0 37.0 37.0 7 36.2015 37.0 37.0 37.0 37.0 37.0 8 36.327 37.0 37.0 37.0 37.0 37.0 9 36.246 37.0 37.0 37.0 37.0 37.0 10-14 36.2217 37.0 37.0 37.0 37.0 37.0 15-19 36.146899999999995 37.0 37.0 37.0 37.0 37.0 20-24 36.18345000000001 37.0 37.0 37.0 37.0 37.0 25-29 36.113550000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.07185 37.0 37.0 37.0 37.0 37.0 35-39 36.05715 37.0 37.0 37.0 37.0 37.0 40-44 36.02635 37.0 37.0 37.0 37.0 37.0 45-49 36.045550000000006 37.0 37.0 37.0 37.0 37.0 50-54 35.98805 37.0 37.0 37.0 37.0 37.0 55-59 35.99205 37.0 37.0 37.0 37.0 37.0 60-64 35.915049999999994 37.0 37.0 37.0 37.0 37.0 65-69 35.93055 37.0 37.0 37.0 37.0 37.0 70-74 35.85315 37.0 37.0 37.0 37.0 37.0 75-79 35.84815 37.0 37.0 37.0 37.0 37.0 80-84 35.83845 37.0 37.0 37.0 37.0 37.0 85-89 35.762649999999994 37.0 37.0 37.0 37.0 37.0 90-94 35.79745 37.0 37.0 37.0 37.0 37.0 95-99 35.76005 37.0 37.0 37.0 37.0 37.0 100-104 35.70435 37.0 37.0 37.0 37.0 37.0 105-109 35.69385 37.0 37.0 37.0 37.0 37.0 110-114 35.54925 37.0 37.0 37.0 37.0 37.0 115-119 35.521750000000004 37.0 37.0 37.0 37.0 37.0 120-124 35.46294999999999 37.0 37.0 37.0 37.0 37.0 125-129 35.49175 37.0 37.0 37.0 37.0 37.0 130-134 35.26475000000001 37.0 37.0 37.0 29.8 37.0 135-139 35.28215 37.0 37.0 37.0 32.2 37.0 140-144 35.147149999999996 37.0 37.0 37.0 29.8 37.0 145-149 34.97765 37.0 37.0 37.0 25.0 37.0 150-151 34.731750000000005 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 2.0 14 2.0 15 1.0 16 0.0 17 0.0 18 1.0 19 2.0 20 2.0 21 1.0 22 2.0 23 1.0 24 6.0 25 7.0 26 6.0 27 13.0 28 13.0 29 21.0 30 36.0 31 49.0 32 81.0 33 124.0 34 254.0 35 661.0 36 2524.0 37 190.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.49060856498873 21.21212121212121 11.119459053343352 30.177811169546708 2 27.474999999999998 25.55 32.65 14.325 3 21.349999999999998 27.725 30.375000000000004 20.549999999999997 4 21.775 34.675 23.875 19.675 5 25.95 36.925000000000004 21.05 16.075 6 20.575 41.375 21.95 16.1 7 19.125 21.55 40.075 19.25 8 20.175 26.1 29.5 24.224999999999998 9 21.75 24.125 31.525 22.6 10-14 21.765 30.345 26.68 21.21 15-19 22.555 28.485 27.894999999999996 21.065 20-24 22.950737684421103 28.08202050512628 28.937234308577143 20.030007501875467 25-29 22.420605151287823 29.287321830457614 27.76194048512128 20.530132533133283 30-34 22.535633908477116 28.477119279819956 28.362090522630655 20.62515628907227 35-39 22.59064766191548 28.267066766691674 27.9869967491873 21.155288822205552 40-44 22.230557639409852 27.701925481370342 29.152288072018006 20.9152288072018 45-49 22.745686421605402 27.95198799699925 27.941985496374095 21.360340085021257 50-54 22.73068267066767 28.217054263565895 28.13703425856464 20.9152288072018 55-59 22.870717679419855 28.367091772943237 28.452113028257063 20.310077519379846 60-64 22.43060765191298 27.731932983245812 28.162040510127532 21.67541885471368 65-69 21.783267490123517 28.569285392808926 28.624293644046606 21.023153473020955 70-74 22.625656414103528 27.49687421855464 28.232058014503625 21.64541135283821 75-79 22.52563140785196 28.962240560140035 27.49687421855464 21.015253813453363 80-84 23.110777694423607 28.177044261065266 27.231807951987996 21.48037009252313 85-89 23.25581395348837 27.84196049012253 28.73718429607402 20.16504126031508 90-94 23.165791447861967 27.85696424106027 27.9869967491873 20.990247561890474 95-99 23.385846461615404 27.80195048762191 27.54688672168042 21.26531632908227 100-104 23.220805201300326 27.71692923230808 27.591897974493623 21.470367591897972 105-109 22.325581395348838 27.80195048762191 29.147286821705425 20.72518129532383 110-114 23.840960240060017 28.387096774193548 27.80195048762191 19.96999249812453 115-119 24.431107776944234 27.966991747936987 27.291822955738937 20.310077519379846 120-124 24.68617154288572 28.42710677669417 27.47186796699175 19.414853713428357 125-129 23.96599149787447 29.18729682420605 26.326581645411352 20.520130032508128 130-134 24.59114778694674 28.047011752938232 27.22680670167542 20.13503375843961 135-139 25.131282820705174 27.646911727931982 27.80195048762191 19.419854963740935 140-144 25.276319079769944 26.80170042510628 27.14678669667417 20.775193798449614 145-149 25.731432858214554 28.092023005751436 26.80170042510628 19.374843710927735 150-151 25.693923480870218 28.319579894973746 26.894223555888974 19.092273068267065 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 0.5 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.0 21 0.5 22 1.0 23 2.5 24 5.0 25 4.0 26 6.0 27 10.5 28 11.0 29 7.5 30 16.0 31 36.0 32 35.5 33 31.0 34 62.5 35 92.5 36 102.5 37 117.0 38 144.5 39 191.5 40 223.5 41 232.0 42 229.0 43 256.0 44 268.0 45 254.0 46 253.0 47 228.5 48 219.0 49 205.0 50 156.0 51 120.0 52 103.5 53 90.0 54 66.5 55 59.5 56 48.5 57 29.5 58 28.5 59 17.0 60 10.5 61 6.5 62 3.0 63 3.5 64 2.0 65 0.5 66 2.5 67 2.5 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17500000000000002 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.025 25-29 0.025 30-34 0.025 35-39 0.025 40-44 0.025 45-49 0.025 50-54 0.025 55-59 0.025 60-64 0.025 65-69 0.015 70-74 0.025 75-79 0.025 80-84 0.025 85-89 0.025 90-94 0.025 95-99 0.025 100-104 0.025 105-109 0.025 110-114 0.025 115-119 0.025 120-124 0.025 125-129 0.025 130-134 0.025 135-139 0.025 140-144 0.025 145-149 0.025 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.875 #Duplication Level Percentage of deduplicated Percentage of total 1 71.01289134438305 48.199999999999996 2 17.974217311233886 24.4 3 6.777163904235728 13.8 4 2.4677716390423576 6.7 5 0.9944751381215469 3.375 6 0.5156537753222836 2.1 7 0.1841620626151013 0.8750000000000001 8 0.0 0.0 9 0.0 0.0 >10 0.07366482504604051 0.5499999999999999 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATGGTCACTCCTGGTGCTGAATGCAAGGACAGGGCCACCCCTGAACAA 11 0.27499999999999997 No Hit AATTACTATCCCATTCTTTTCCGTGGTGTCAACGGAACAGTTGCCCATGA 11 0.27499999999999997 No Hit ATTCAAGATATCTGGTTTTGATTTGAGAGACGCACTCGTGGGGCATTCGG 7 0.17500000000000002 No Hit GGAGGATACCTTAACTGAAAATGTGGCTAAGTTTTACATTGCTCAAAGTA 7 0.17500000000000002 No Hit GTGGAAACTTGTAAGATCAAAATGTGTTTTAAGGGACACTTGGACTACTT 7 0.17500000000000002 No Hit ATTGGTTCGGTTAATGGGTTGGTGTTTTTGAGACATTCGGAGAGGAATCT 7 0.17500000000000002 No Hit CTCGAAGCGAGAGCCTTTCTCCGAGAAAAAATGGCTGTAGAGATGGCTTT 7 0.17500000000000002 No Hit CAAGCATTACATGGCAGAAGCTATGAAGCCATGTTGAAGTTGATTATTTC 6 0.15 No Hit GAACATGATTACGGATTCTGATCTGAGAGAAATTGATCTTTATTCATTTC 6 0.15 No Hit CTGTTATCTCTGCTAAGTCCAAGGACGAGAAGAAATCCCTTAAGGAACTT 6 0.15 No Hit AAAAGGGAAAAAAAAGGAGAGAGAAAAATAATCAGAGATGGGGTTGTTTA 6 0.15 No Hit CAGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 6 0.15 No Hit AACAAGAGGTAATTCAATTAGGGAGAATATGTTGTTAAGGGCATTGAACT 6 0.15 No Hit TCTCTTCATCAACAGCGCTCCTCCACCGTTGATCACCGTGTCTCTTCCAA 6 0.15 No Hit CTGGATCAGGAATGACACATGAACAACATCTCCAGTTTTTTCATTTCCTT 6 0.15 No Hit TCCCTCTCCCTGTCTCTCTTGTCTTCGTGTTGAAATGAAAACCCACTTCT 6 0.15 No Hit GTCATTCTGGTGTATAAATTCAAAAGGCAAGGGACTGATTAAAATTCAAA 6 0.15 No Hit TGCAATATTCTATCGGAGTGAGGATGAATGGGGAATTAGCAGCACCGGAG 6 0.15 No Hit AGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATC 6 0.15 No Hit AGTAGTTTGATAAATCGGTGGAATGGGAAGCCCTACTCACAGAGGTACTA 6 0.15 No Hit GAGAAAGAAGCCAACATTAAGCCTGATCCTGATGTTGATGTCTTCATGAA 6 0.15 No Hit TAATACTGTTATGGCTGGCAAGTTAGCATTTACGTTGACATCTCCTCGAG 5 0.125 No Hit CATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAA 5 0.125 No Hit GGTTTTACTAGACAAGATGTGGGATGATGTTGTTGCTGGACCTCAGCCAG 5 0.125 No Hit CTTAGCCTTCTAAGAACAGTGAAACTGATCCACTCGTCACAAAATATAAC 5 0.125 No Hit GGGAGATGGTTACAAGTTTTATCCCTTTTCATTTGCATCAGTAAATGATG 5 0.125 No Hit TATGCAGTTTTGCATCTGAAGGTGGAATACATTGTGGTCATTGGACACAG 5 0.125 No Hit CACCTATCCATCTGTTTCTCTCTATTTCATCTCCATAGAAAAGAAGAGAA 5 0.125 No Hit CTTCAACCCCCTCAACTTTGCACCCACAATTGAGGCCAAAGAGAAAGAGC 5 0.125 No Hit TATATGATAGTTTTCCTTGTCCTTATTTATGTTTTCCTTGTCCTTATTTA 5 0.125 No Hit GTTCATCCATTTAAGCTTAATCAATCAATCATCATGTCTAGCACCTGCGA 5 0.125 No Hit CCCCTACAACGACACTTCTCTCCCCTTCCATGGCGAGAACTCTCCTTTCA 5 0.125 No Hit GTCTTTGATTGATATGTATAGTAAATGTGGGAGTTTGGAGGATGCGCGGT 5 0.125 No Hit CGTACGTACAACCTGGACAACATGATGGATGGTTTCTACATTGCTCCTGC 5 0.125 No Hit TGGCTGTGCGTTTCTCTTCAATAGGAGAAGAATGGCTATTCTCTCTCTCT 5 0.125 No Hit GAAACCGCCTGCTGTTGAGCAGGGTTTGCTAAAGATGAAATCTGAAGAGA 5 0.125 No Hit CTAATGACCAGCTCGATGCACTGCGGCAGTTCTGCTGCCAAACACTTAAG 5 0.125 No Hit CACTTTCTTTTAAAATTTATTGGTCCTCTCTCTCTCTCTCTAATCTTTAT 5 0.125 No Hit GAGAGATTAAGATGATGCCTTTTGGAGCTGGAAGGAGAATTTGCCCTGGT 5 0.125 No Hit GTTCAGCCTGGACACAGGGAGATTGAACCCAGAAACATACCATTTCTTTG 5 0.125 No Hit CTTGGTTGGAAGATTTTTCTGATCAAGTGGATGAAAAGGTGGTATTCAAG 5 0.125 No Hit CACTACTCTCTCCCCTCAACCACCAGTAGAGTTGTTGTTTTTCTCTTTCC 5 0.125 No Hit GCAAGGAGACACTAACAAGGATGGTTTGCTAAGCATGCAAGAGTTTTTGG 5 0.125 No Hit AGTAAACTGAACAGAGAATTGTCCATCAAACCAATGGCATCGTCATCTCC 5 0.125 No Hit GTTTCCGCAGCAATTGACGAGCTCGAGAAGCTCCGCCAGCCTCTCTCCAT 5 0.125 No Hit AGTCTTGGCAACAGTATTGCTTCAGATGATTCAAGGTTATCCGAATTTCA 5 0.125 No Hit CTGCTCCCGAGATCATTTGCAGTCAAGGATGAGATCTACTGCTTGTTTGA 5 0.125 No Hit ATTCAAAATCCCACCTTCCCCGATAACAAAGAAGCGACTGCTTTTTGATA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0125 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.1125 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.275 0.0 0.0 0.0 0.0 84-85 0.4375 0.0 0.0 0.0 0.0 86-87 0.5 0.0 0.0 0.0 0.0 88-89 0.5625 0.0 0.0 0.0 0.0 90-91 0.675 0.0 0.0 0.0 0.0 92-93 0.9125 0.0 0.0 0.0 0.0 94-95 1.0625 0.0 0.0 0.0 0.0 96-97 1.2125 0.0 0.0 0.0 0.0 98-99 1.275 0.0 0.0 0.0 0.0 100-101 1.6 0.0 0.0 0.0 0.0 102-103 1.9125 0.0 0.0 0.0 0.0 104-105 2.4749999999999996 0.0 0.0 0.0 0.0 106-107 2.7375 0.0 0.0 0.0 0.0 108-109 2.9749999999999996 0.0 0.0 0.0 0.0 110-111 3.3 0.0 0.0 0.0 0.0 112-113 3.5625 0.0 0.0 0.0 0.0 114-115 3.8499999999999996 0.0 0.0 0.0 0.0 116-117 4.35 0.0 0.0 0.0 0.0 118-119 4.8125 0.0 0.0 0.0 0.0 120-121 5.375 0.0 0.0 0.0 0.0 122-123 5.675 0.0 0.0 0.0 0.0 124-125 6.15 0.0 0.0 0.0 0.0 126-127 6.887499999999999 0.0 0.0 0.0 0.0 128-129 7.387499999999999 0.0 0.0 0.0 0.0 130-131 7.9 0.0 0.0 0.0 0.0 132-133 8.3875 0.0 0.0 0.0 0.0 134-135 9.0125 0.0 0.0 0.0 0.0 136-137 9.6875 0.0 0.0 0.0 0.0 138-139 10.35 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAACCAC 10 0.006830828 145.0 3 ACGTTTC 10 0.006830828 145.0 145 AACGAAC 10 0.006830828 145.0 1 AACCTTT 10 0.006830828 145.0 5 AACCACA 10 0.006830828 145.0 4 GAACCTT 10 0.006830828 145.0 4 CGAACCT 10 0.006830828 145.0 3 CCTTTGG 10 0.006830828 145.0 7 GAGAGGG 10 0.006830828 145.0 3 CTTTGGG 10 0.006830828 145.0 8 ACGAACC 10 0.006830828 145.0 2 AAGTTCT 10 0.006830828 145.0 5 >>END_MODULE Read 514008 spots for SRR13695444.sra Written 514008 spots for SRR13695444.sra Read 514008 spots for SRR13695444.sra Written 514008 spots for SRR13695444.sra Read 514008 spots for SRR13695444.sra Written 514008 spots for SRR13695444.sra Read 514008 spots for SRR13695444.sra Written 514008 spots for SRR13695444.sra Read 514008 spots for SRR13695444.sra Written 514008 spots for SRR13695444.sra Read 514008 spots for SRR13695444.sra Written 514008 spots for SRR13695444.sra Read 514008 spots for SRR13695444.sra Written 514008 spots for SRR13695444.sra Read 514008 spots for SRR13695444.sra Written 514008 spots for SRR13695444.sra Read 514008 spots for SRR13695444.sra Written 514008 spots for SRR13695444.sra Read 514008 spots for SRR13695444.sra Written 514008 spots for SRR13695444.sra Read 514008 spots for SRR13695444.sra Written 514008 spots for SRR13695444.sra Read 514008 spots for SRR13695444.sra Written 514008 spots for SRR13695444.sra Read 514008 spots for SRR13695444.sra Written 514008 spots for SRR13695444.sra Read 514024 spots for SRR13695444.sra Written 514024 spots for SRR13695444.sra Read 514008 spots for SRR13695444.sra Written 514008 spots for SRR13695444.sra Read 514008 spots for SRR13695444.sra Written 514008 spots for SRR13695444.sra Read 514008 spots for SRR13695444.sra Written 514008 spots for SRR13695444.sra Read 514008 spots for SRR13695444.sra Written 514008 spots for SRR13695444.sra Read 514008 spots for SRR13695444.sra Written 514008 spots for SRR13695444.sra Read 514008 spots for SRR13695444.sra Written 514008 spots for SRR13695444.sra SRR ids: ['SRR13695444.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_xutg0kgd SRR13695444.sra spots: 10280176 blocks: [[1, 514008], [514009, 1028016], [1028017, 1542024], [1542025, 2056032], [2056033, 2570040], [2570041, 3084048], [3084049, 3598056], [3598057, 4112064], [4112065, 4626072], [4626073, 5140080], [5140081, 5654088], [5654089, 6168096], [6168097, 6682104], [6682105, 7196112], [7196113, 7710120], [7710121, 8224128], [8224129, 8738136], [8738137, 9252144], [9252145, 9766152], [9766153, 10280176]] SRR13695444 file size 3471953 SRR13695444 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695444 SRR13695444_1.fastq SRR13695444_2.fastq Input file: SRR13695444_1.fastq Paired file: SRR13695444_2.fastq trimmed: SRR13695444-trimmed-pair1.fastq, SRR13695444-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 03:34:37 2025 >> started Wed Feb 12 03:34:48 2025 >> done (10.911s) 10280176 read pairs processed; of these: 84 ( 0.00%) short read pairs filtered out after trimming by size control 379 ( 0.00%) empty read pairs filtered out after trimming by size control 10279713 (100.00%) read pairs available; of these: 1376057 (13.39%) trimmed read pairs available after processing 8903656 (86.61%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 3 0.00% 19 3 0.00% 20 2 0.00% 21 1 0.00% 22 3 0.00% 23 5 0.00% 24 5 0.00% 25 0 0.00% 26 4 0.00% 27 0 0.00% 28 4 0.00% 29 2 0.00% 30 1 0.00% 31 1 0.00% 32 9 0.00% 33 1 0.00% 34 6 0.00% 35 4 0.00% 36 4 0.00% 37 6 0.00% 38 9 0.00% 39 5 0.00% 40 14 0.00% 41 6 0.00% 42 15 0.00% 43 18 0.00% 44 16 0.00% 45 19 0.00% 46 28 0.00% 47 36 0.00% 48 39 0.00% 49 61 0.00% 50 50 0.00% 51 79 0.00% 52 64 0.00% 53 102 0.00% 54 137 0.00% 55 142 0.00% 56 151 0.00% 57 205 0.00% 58 189 0.00% 59 244 0.00% 60 310 0.00% 61 317 0.00% 62 421 0.00% 63 472 0.00% 64 526 0.01% 65 629 0.01% 66 767 0.01% 67 815 0.01% 68 885 0.01% 69 1040 0.01% 70 1220 0.01% 71 1410 0.01% 72 1588 0.02% 73 1854 0.02% 74 2106 0.02% 75 2236 0.02% 76 2479 0.02% 77 2675 0.03% 78 2860 0.03% 79 3243 0.03% 80 3573 0.03% 81 3919 0.04% 82 4269 0.04% 83 4766 0.05% 84 5300 0.05% 85 5381 0.05% 86 5616 0.05% 87 5957 0.06% 88 6427 0.06% 89 6593 0.06% 90 6957 0.07% 91 7646 0.07% 92 7928 0.08% 93 8672 0.08% 94 9203 0.09% 95 9588 0.09% 96 9887 0.10% 97 10009 0.10% 98 10656 0.10% 99 11078 0.11% 100 11596 0.11% 101 11844 0.12% 102 12716 0.12% 103 13017 0.13% 104 13812 0.13% 105 14041 0.14% 106 14519 0.14% 107 14724 0.14% 108 15080 0.15% 109 15722 0.15% 110 15943 0.16% 111 16473 0.16% 112 17498 0.17% 113 17910 0.17% 114 18208 0.18% 115 19332 0.19% 116 19415 0.19% 117 19645 0.19% 118 20866 0.20% 119 20683 0.20% 120 21606 0.21% 121 22099 0.21% 122 22820 0.22% 123 23354 0.23% 124 23845 0.23% 125 23777 0.23% 126 24689 0.24% 127 24465 0.24% 128 24915 0.24% 129 25043 0.24% 130 26636 0.26% 131 26558 0.26% 132 26861 0.26% 133 28081 0.27% 134 28066 0.27% 135 28716 0.28% 136 29083 0.28% 137 29449 0.29% 138 29740 0.29% 139 30345 0.30% 140 30024 0.29% 141 30371 0.30% 142 31577 0.31% 143 31393 0.31% 144 32502 0.32% 145 32900 0.32% 146 33219 0.32% 147 33921 0.33% 148 34509 0.34% 149 34278 0.33% 150 35200 0.34% 151 8903656 86.61% 10279713 reads passed initial QC criterion=sequence-density sequence-density=0.72 sequence-density-rank=1 fanout-score=2.10 fanout-score-rank=24 prefix-density=0.76 prefix-fanout=2.0 sequence=TTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGG criterion=fanout-score sequence-density=0.02 sequence-density-rank=31 fanout-score=244.07 fanout-score-rank=1 prefix-density=0.25 prefix-fanout=16.7 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTG criterion=sequence-density sequence-density=1.09 sequence-density-rank=1 fanout-score=2.12 fanout-score-rank=28 prefix-density=1.10 prefix-fanout=2.1 sequence=CCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAATCATCATGTCTAGCACCTGCGACACCTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAA criterion=fanout-score sequence-density=0.08 sequence-density-rank=28 fanout-score=74.49 fanout-score-rank=1 prefix-density=0.58 prefix-fanout=10.0 sequence=AAAAGAAAAGAAAA SRR13695444 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 03:35:30 Started mapping on | Feb 12 03:35:30 Finished on | Feb 12 03:36:52 Mapping speed, Million of reads per hour | 451.30 Number of input reads | 10279713 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 9508733 Uniquely mapped reads % | 92.50% Average mapped length | 293.10 Number of splices: Total | 9230215 Number of splices: Annotated (sjdb) | 9014679 Number of splices: GT/AG | 9034015 Number of splices: GC/AG | 145662 Number of splices: AT/AC | 5384 Number of splices: Non-canonical | 45154 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.03% Deletion average length | 2.96 Insertion rate per base | 0.02% Insertion average length | 2.35 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 227149 % of reads mapped to multiple loci | 2.21% Number of reads mapped to too many loci | 28221 % of reads mapped to too many loci | 0.27% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.92% % of reads unmapped: other | 0.10% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 543950 543950 543950 N_multimapping 227149 227149 227149 N_noFeature 427859 9244662 588892 N_ambiguous 161270 1139 57343 UnstrandedReadsAssigned:8919604 PositiveStrandReadsAssigned:262932 NegativeStrandReadsAssigned:8862498 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695444 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695444-trimmed-pair1.fastq SRR13695444-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 10,279,713 reads, 8,832,865 reads pseudoaligned [quant] estimated average fragment length: 253.804 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,232 rounds 52401 SRR13695444.ke.tsv 34699 SRR13695444.se.tsv 87100 total ==> SRR13695444.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1765.2 568 32.2176 Potri.005G024800.1.v4.1 1035 782.196 149 19.0725 Potri.004G059700.1.v4.1 961 708.312 1 0.141356 Potri.007G009000.2.v4.1 1416 1163.2 0 0 Potri.003G141000.2.v4.1 2943 2690.2 599.028 22.2947 Potri.016G087400.1.v4.1 270 89.2002 533 598.273 Potri.015G069301.1.v4.1 564 324.461 0 0 Potri.010G195200.1.v4.1 1773 1520.2 185 12.1846 Potri.012G127500.1.v4.1 977 724.244 199 27.511 ==> SRR13695444.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 68 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 103 Potri.001G212900.v4.1 16 Potri.001G182400.v4.1 1 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 2 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 4 SRR13695444 completed mapping pipeline successfully