Starting /dee2/code/volunteer_pipeline.sh SRR13695445 current disk space = 3048992735232 free memory = 988648300 SRR13695445 SRAfilesize bfcbf299b0a2665f44bd797b59636b2a SRR13695445.sra SRR13695445.sra file validated SRR13695445 is paired end SRR13695445 is conventional basespace SRR13695445 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695445_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5595 37.0 37.0 37.0 37.0 37.0 2 36.1745 37.0 37.0 37.0 37.0 37.0 3 36.485 37.0 37.0 37.0 37.0 37.0 4 36.5235 37.0 37.0 37.0 37.0 37.0 5 36.5445 37.0 37.0 37.0 37.0 37.0 6 36.548 37.0 37.0 37.0 37.0 37.0 7 36.411 37.0 37.0 37.0 37.0 37.0 8 36.4495 37.0 37.0 37.0 37.0 37.0 9 36.541 37.0 37.0 37.0 37.0 37.0 10-14 36.5392 37.0 37.0 37.0 37.0 37.0 15-19 36.464800000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.3737 37.0 37.0 37.0 37.0 37.0 25-29 36.407 37.0 37.0 37.0 37.0 37.0 30-34 36.3913 37.0 37.0 37.0 37.0 37.0 35-39 36.3745 37.0 37.0 37.0 37.0 37.0 40-44 36.3364 37.0 37.0 37.0 37.0 37.0 45-49 36.3745 37.0 37.0 37.0 37.0 37.0 50-54 36.3006 37.0 37.0 37.0 37.0 37.0 55-59 36.302099999999996 37.0 37.0 37.0 37.0 37.0 60-64 36.3237 37.0 37.0 37.0 37.0 37.0 65-69 36.2364 37.0 37.0 37.0 37.0 37.0 70-74 36.2452 37.0 37.0 37.0 37.0 37.0 75-79 36.204100000000004 37.0 37.0 37.0 37.0 37.0 80-84 36.137100000000004 37.0 37.0 37.0 37.0 37.0 85-89 36.1982 37.0 37.0 37.0 37.0 37.0 90-94 36.1274 37.0 37.0 37.0 37.0 37.0 95-99 36.0658 37.0 37.0 37.0 37.0 37.0 100-104 36.011199999999995 37.0 37.0 37.0 37.0 37.0 105-109 36.0437 37.0 37.0 37.0 37.0 37.0 110-114 36.0118 37.0 37.0 37.0 37.0 37.0 115-119 36.0101 37.0 37.0 37.0 37.0 37.0 120-124 35.8777 37.0 37.0 37.0 37.0 37.0 125-129 35.87519999999999 37.0 37.0 37.0 37.0 37.0 130-134 35.8212 37.0 37.0 37.0 37.0 37.0 135-139 35.8355 37.0 37.0 37.0 37.0 37.0 140-144 35.7165 37.0 37.0 37.0 37.0 37.0 145-149 35.468 37.0 37.0 37.0 37.0 37.0 150-151 35.25375 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 0.0 21 1.0 22 2.0 23 0.0 24 2.0 25 3.0 26 10.0 27 10.0 28 19.0 29 28.0 30 30.0 31 40.0 32 37.0 33 80.0 34 135.0 35 357.0 36 2934.0 37 311.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 54.074999999999996 12.925 4.475 28.525 2 23.086606243705944 12.588116817724067 36.05236656596173 28.272910372608255 3 15.9 21.7 30.825000000000003 31.574999999999996 4 21.15 27.55 25.55 25.75 5 22.325 36.35 23.1 18.224999999999998 6 18.15 36.05 24.525 21.275 7 14.05 26.075 43.65 16.225 8 16.075 24.525 35.025 24.375 9 16.650000000000002 22.375 35.699999999999996 25.275 10-14 20.005 29.48 27.57 22.945 15-19 20.09 28.415000000000003 27.779999999999998 23.715 20-24 19.725 28.810000000000002 27.889999999999997 23.575 25-29 20.035 28.775000000000002 28.605000000000004 22.585 30-34 19.805 28.43 28.189999999999998 23.575 35-39 20.41 28.439999999999998 28.244999999999997 22.905 40-44 20.015 28.215 28.32 23.45 45-49 20.25 28.13 28.03 23.59 50-54 19.71 28.28 28.139999999999997 23.87 55-59 20.655 28.515 27.445000000000004 23.385 60-64 20.72 28.365000000000002 27.334999999999997 23.580000000000002 65-69 19.63 28.299999999999997 29.025000000000002 23.044999999999998 70-74 20.46 28.315 27.915 23.31 75-79 20.064999999999998 28.499999999999996 27.800000000000004 23.635 80-84 19.965 28.794999999999998 27.544999999999998 23.695 85-89 20.39 28.4 27.74 23.47 90-94 20.195 28.485 27.735 23.585 95-99 19.67 28.845 28.09 23.395 100-104 20.66 28.105000000000004 27.694999999999997 23.54 105-109 20.96 28.794999999999998 26.365 23.880000000000003 110-114 19.6 29.115000000000002 28.12 23.165 115-119 21.375 28.194999999999997 27.315 23.115 120-124 20.64 29.145 26.465 23.75 125-129 21.105 27.415 27.279999999999998 24.2 130-134 21.52 28.084999999999997 27.015 23.380000000000003 135-139 21.959999999999997 28.58 26.21 23.25 140-144 21.005 27.865000000000002 26.755000000000003 24.375 145-149 21.475 27.145000000000003 26.645000000000003 24.735 150-151 21.55 27.675 26.7125 24.0625 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 1.0 18 0.5 19 0.0 20 0.0 21 1.0 22 2.5 23 2.5 24 2.5 25 5.5 26 8.0 27 11.0 28 13.0 29 14.0 30 20.0 31 31.0 32 42.0 33 52.0 34 55.5 35 76.0 36 104.0 37 118.0 38 145.0 39 180.5 40 205.5 41 223.0 42 222.5 43 233.0 44 267.0 45 260.0 46 240.0 47 221.5 48 200.5 49 194.0 50 175.0 51 139.5 52 127.0 53 110.5 54 71.5 55 55.0 56 45.0 57 34.5 58 23.5 59 14.5 60 13.0 61 12.0 62 11.5 63 7.0 64 3.0 65 2.0 66 0.5 67 0.0 68 0.5 69 0.5 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.7000000000000001 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.64999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 67.51713632901752 44.324999999999996 2 20.67783701447068 27.150000000000002 3 7.044935262757044 13.875000000000002 4 2.77989337395278 7.3 5 1.2185833968012185 4.0 6 0.456968773800457 1.7999999999999998 7 0.15232292460015232 0.7000000000000001 8 0.07616146230007616 0.4 9 0.07616146230007616 0.44999999999999996 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCCGATTCAGCATCCGAATCCAGAAGCTAAAAACAAAAACAAAGTAGAA 9 0.22499999999999998 No Hit GCTCAACATGCTCGTTGACAAAATGTACAATTAAAGAGAAAAAAGATGTA 9 0.22499999999999998 No Hit AATAATTCTGATAATTTACCGTTTTTGAGCATAATCGTACCGGTATATCC 8 0.2 No Hit GTGCAAGAATGCCCTATAAAATTACATAAATCGGAAAAATCAGCTTCTGT 8 0.2 No Hit GGGTAGCTTCCACATAGTCCAGTAGCGTCCATCATAGTACCCTGGTGAGC 7 0.17500000000000002 No Hit CCTTGATTCCCCGATGAACCTTGGCAAAAGACAGGCAAGACCAATTCCAA 7 0.17500000000000002 No Hit CCGAATAAGAAATTCTTAGAAATATCAAGTCTTGTCAAATTCTGAAGCGA 7 0.17500000000000002 No Hit AATATCAATCACAAAACGGTACTTCACGTCGTTCTTTACCATCCTCTCAA 7 0.17500000000000002 No Hit GCATTGCAAGAGGTTGCCCAATCCCTCCAGCAGCACCCAAGATAGCCACT 6 0.15 No Hit GCCCTTTATGCATTTCCAATAAGAAAAGTCAACCAATTGGCCATCTTTTC 6 0.15 No Hit CAAGCATTCAACATTCATCCTCACAAGAAATTAGACAATCAGTCTCGTCA 6 0.15 No Hit ATGCGCTTCAAGGTACAAAACCTGGCCAAGAACTCCAGTCTCCCAAAACA 6 0.15 No Hit GTTTGCCCGGCGAGAGGGTCGAGCTTTGTGGTGCAAGTGTCCCCTGCCAC 6 0.15 No Hit GTCGAATAATCTTTCTTAGGGTCTGTGGAAGGAGAGGGCTGACTAGAGCT 6 0.15 No Hit ATATAACAAAATCTTATTGGTCAATCTGAGGGCACAGCATTCACACATGA 6 0.15 No Hit ATCACACTTCATATAACACAAAAGCAGCACCGTCTAAAAATACAGTACTA 6 0.15 No Hit TTCACACCAAGACCAAGGTGATCGCACACAACCTTAACACACATTCCACT 6 0.15 No Hit CCTCATGATCTCAGACTCCGCACTTTCTTCACCAAATGCTTTTATAAGCA 6 0.15 No Hit GTGACAGTAACATAAAACAGAACAGTGAACAGTAACAAATAGTAACACAA 6 0.15 No Hit CCCACCACCTGCATTCGTAGACTTGTGATGTCGATATTCATTCTACCAAC 6 0.15 No Hit GGAGGCATGTTGTTTGCTTCCCCCACACATGTCATTTGTGCCAGCACTTG 5 0.125 No Hit AGCGATGGCGCATCTGACATTACAATGGTGTCAGGCAACTCGGGTGCATT 5 0.125 No Hit GACAATTAAAAAACACACTTTGGCATGAAACATGAAAGACAAATGACTTA 5 0.125 No Hit CCATCTCCAAACTCCTCCACAAATTTCTGCTTCCATAAATCATTGTTCGA 5 0.125 No Hit GCATGTTTTTCTTTTAGCTTTGCAGATAGTTGAGAGACCTCTTCCTCGAC 5 0.125 No Hit GACAGAACCCACGGCTTGCATCACTGATACGGAGGTGAAGTTCTCTGAGG 5 0.125 No Hit TGTGATCTCATATTTGGTGCTTAGGTATTTATCTGTTGCAGGATTCCATT 5 0.125 No Hit TTTTTTTTTTGTGTTGCATCCAGACAGGAAAAGTAATATTTAGATTTCAC 5 0.125 No Hit GGATGCTTTAGAGAAAAATCACCAATTTCATGCCTCCACCATCTCCTCTA 5 0.125 No Hit CTCAGCCTTTATCATGTAATCTGGACAAGAATCCTCTCGAATCCAGTTTG 5 0.125 No Hit GTACGGATATCCTGATGGAAAAAACTTGTTCAAGAAAGATTCAACCACTC 5 0.125 No Hit GGGTCCAATAGGTGGTGGCTTAGGTTTTGGAGCTTCAGCTACAGCTGGTT 5 0.125 No Hit CTCTTCTCGCTAGGTTCATACTTCCCCCTTCGAAGCTGGTATCGATGAGT 5 0.125 No Hit GCCATCCTCAGCAGGAGACTCAGGGAGCTGAATGGAAGAGCGGCTGGAAG 5 0.125 No Hit GTTGCTGGTAGCCTTGTCCCTGCGAATATGCTGCTCCCTAGTTTGCATCG 5 0.125 No Hit CAGCAAATGTCTTCTGTCCGCAAAAATCTCAAACAAAAGCAGCAATTATC 5 0.125 No Hit AGCATTCGAAACTAAAACAGACAATACTTGATAATTGTTCTAGCGATAGC 5 0.125 No Hit TTTTAATTCCATTCAGAGCAGCACAGGCTATGTCTGTAACTGAAAGATCT 5 0.125 No Hit CATTGCTCATCAATCTCTTGTCTGGTACTTGTGATTACAAGTTCTGCAGC 5 0.125 No Hit CGGAGAATGGCTTGGATTACTGGCTTGTTAGGAACTCATGGGGCACAGAA 5 0.125 No Hit ATCGAAGGTGGAGATTTGCGGTGGCGTGAGAGACTGAGGAGTGGTTGCGG 5 0.125 No Hit GCACTCAACTTTGCTTGCTTCTAATCTTAAAGGCGCCGCCCACAATCACG 5 0.125 No Hit GTCTCCAAATGGCTCTTCCTTTGTTACCCAGAAGGTTGAATAACCAAAAA 5 0.125 No Hit GCACTGCCTACTTGAAAGTCAAGAAGCCTTTTTTGTTTCTGGTAATAATC 5 0.125 No Hit GTCACATTTGGCAGATCAACTAGTCCATGTTCTTGGGCAGTTTTGAGCCT 5 0.125 No Hit AGTAAACTTAAAAAGAAACCACTTCTGTGCTTGACCTTTGTAATTGGTAC 5 0.125 No Hit CATTATAACTGGTATCACAATCCACCAACTCCTGCTCAGACAAAGCGATC 5 0.125 No Hit GTTCTTCCATTTTCCTCTCCATCCAAAACCTGCAAGCGTGTCTTAATTGT 5 0.125 No Hit CTCCTTTCTTAGCAAGCTGCTCAATGTTATATCCAGGAGCAGGGTCATTA 5 0.125 No Hit CTACCATCTTTCGGAGAAGCCCCATTTTTGCTTTTGCTTTTGCTTTTTTT 5 0.125 No Hit GTGCAGTTTGCCACCCTCCGGTAGTTTCATGAGAGGTTTGGCCCAAGAAA 5 0.125 No Hit GGGAAACTCATATGAAAATTTACTACTAGTTGTACTCCATTGAAATTCAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0875 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.1625 0.0 0.0 0.0 0.0 74-75 0.23750000000000002 0.0 0.0 0.0 0.0 76-77 0.2875 0.0 0.0 0.0 0.0 78-79 0.5 0.0 0.0 0.0 0.0 80-81 0.625 0.0 0.0 0.0 0.0 82-83 0.875 0.0 0.0 0.0 0.0 84-85 1.1375 0.0 0.0 0.0 0.0 86-87 1.275 0.0 0.0 0.0 0.0 88-89 1.4500000000000002 0.0 0.0 0.0 0.0 90-91 1.7 0.0 0.0 0.0 0.0 92-93 1.8250000000000002 0.0 0.0 0.0 0.0 94-95 1.9625 0.0 0.0 0.0 0.0 96-97 2.05 0.0125 0.0 0.0 0.0 98-99 2.225 0.025 0.0 0.0 0.0 100-101 2.575 0.025 0.0 0.0 0.0 102-103 2.9375 0.025 0.0 0.0 0.0 104-105 3.2375 0.025 0.0 0.0 0.0 106-107 3.4875 0.025 0.0 0.0 0.0 108-109 3.9 0.025 0.0 0.0 0.0 110-111 4.5875 0.025 0.0 0.0 0.0 112-113 4.9375 0.025 0.0 0.0 0.0 114-115 5.475 0.025 0.0 0.0 0.0 116-117 5.9625 0.025 0.0 0.0 0.0 118-119 6.387499999999999 0.025 0.0 0.0 0.0 120-121 6.6875 0.025 0.0 0.0 0.0 122-123 7.125 0.025 0.0 0.0 0.0 124-125 7.6875 0.025 0.0 0.0 0.0 126-127 8.337499999999999 0.025 0.0 0.0 0.0 128-129 8.9875 0.025 0.0 0.0 0.0 130-131 9.825 0.025 0.0 0.0 0.0 132-133 10.3125 0.025 0.0 0.0 0.0 134-135 11.05 0.025 0.0 0.0 0.0 136-137 11.6 0.025 0.0 0.0 0.0 138-139 12.2 0.025 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTGCT 10 0.006830828 145.0 1 TATGTGG 10 0.006830828 145.0 6 AATTATG 10 0.006830828 145.0 3 TCCAGCT 10 0.006830828 145.0 145 ATGTGGA 10 0.006830828 145.0 7 ATTATGT 10 0.006830828 145.0 4 ATTGCTC 10 0.006830828 145.0 2 TGTGGAG 10 0.006830828 145.0 8 GCTCATC 10 0.006830828 145.0 5 TTATGTG 10 0.006830828 145.0 5 >>END_MODULE SRR13695445 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695445_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.87625 37.0 37.0 37.0 37.0 37.0 2 35.893 37.0 37.0 37.0 37.0 37.0 3 35.9705 37.0 37.0 37.0 37.0 37.0 4 36.1285 37.0 37.0 37.0 37.0 37.0 5 36.212 37.0 37.0 37.0 37.0 37.0 6 36.13 37.0 37.0 37.0 37.0 37.0 7 36.137 37.0 37.0 37.0 37.0 37.0 8 36.2675 37.0 37.0 37.0 37.0 37.0 9 36.205 37.0 37.0 37.0 37.0 37.0 10-14 36.174800000000005 37.0 37.0 37.0 37.0 37.0 15-19 36.1097 37.0 37.0 37.0 37.0 37.0 20-24 36.06905 37.0 37.0 37.0 37.0 37.0 25-29 35.99204999999999 37.0 37.0 37.0 37.0 37.0 30-34 35.984249999999996 37.0 37.0 37.0 37.0 37.0 35-39 35.96424999999999 37.0 37.0 37.0 37.0 37.0 40-44 35.93405 37.0 37.0 37.0 37.0 37.0 45-49 35.897949999999994 37.0 37.0 37.0 37.0 37.0 50-54 35.87195 37.0 37.0 37.0 37.0 37.0 55-59 35.85535 37.0 37.0 37.0 37.0 37.0 60-64 35.79515 37.0 37.0 37.0 37.0 37.0 65-69 35.7153 37.0 37.0 37.0 37.0 37.0 70-74 35.76715 37.0 37.0 37.0 37.0 37.0 75-79 35.73395000000001 37.0 37.0 37.0 37.0 37.0 80-84 35.67575000000001 37.0 37.0 37.0 37.0 37.0 85-89 35.620349999999995 37.0 37.0 37.0 37.0 37.0 90-94 35.57335 37.0 37.0 37.0 37.0 37.0 95-99 35.53315 37.0 37.0 37.0 37.0 37.0 100-104 35.56224999999999 37.0 37.0 37.0 37.0 37.0 105-109 35.546949999999995 37.0 37.0 37.0 37.0 37.0 110-114 35.391949999999994 37.0 37.0 37.0 37.0 37.0 115-119 35.41955 37.0 37.0 37.0 37.0 37.0 120-124 35.242850000000004 37.0 37.0 37.0 34.6 37.0 125-129 35.20445 37.0 37.0 37.0 32.2 37.0 130-134 35.05945 37.0 37.0 37.0 29.8 37.0 135-139 35.018100000000004 37.0 37.0 37.0 25.0 37.0 140-144 34.84974999999999 37.0 37.0 37.0 25.0 37.0 145-149 34.6591 37.0 37.0 37.0 25.0 37.0 150-151 34.179500000000004 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 3.0 14 3.0 15 3.0 16 3.0 17 0.0 18 0.0 19 1.0 20 2.0 21 4.0 22 2.0 23 7.0 24 7.0 25 8.0 26 9.0 27 12.0 28 18.0 29 33.0 30 43.0 31 69.0 32 87.0 33 143.0 34 234.0 35 683.0 36 2484.0 37 141.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 57.11417816813049 18.74529485570891 5.872020075282309 18.268506900878293 2 29.549999999999997 19.2 31.65 19.6 3 22.275 25.174999999999997 33.35 19.2 4 24.925 34.375 22.85 17.849999999999998 5 24.8 36.725 20.150000000000002 18.325 6 21.15 38.85 21.575 18.425 7 21.675 23.799999999999997 35.125 19.400000000000002 8 17.825 27.400000000000002 30.075000000000003 24.7 9 22.275 23.674999999999997 30.525000000000002 23.525 10-14 23.425 28.265 27.794999999999998 20.515 15-19 23.345 28.03 28.12 20.505000000000003 20-24 23.095773943485874 28.35708927231808 27.376844211052763 21.170292573143286 25-29 23.34583645911478 26.966741685421354 28.95223805951488 20.735183795948984 30-34 22.85571392848212 27.366841710427607 28.962240560140035 20.81520380095024 35-39 22.61065266316579 28.292073018254566 28.60715178794699 20.49012253063266 40-44 23.10077519379845 28.127031757939484 27.76694173543386 21.005251312828207 45-49 22.440610152538135 27.53188297074269 28.927231807951987 21.10027506876719 50-54 22.245561390347586 28.37209302325581 28.70217554388597 20.68017004251063 55-59 23.360840210052515 27.961990497624406 27.906976744186046 20.770192548137032 60-64 23.245811452863215 27.826956739184794 27.936984246061513 20.990247561890474 65-69 23.664732946589318 27.99059811962393 27.485497099419888 20.859171834366876 70-74 23.80595148787197 27.691922980745186 27.651912978244564 20.850212553138284 75-79 23.58089522380595 27.426856714178545 27.866966741685424 21.12528132033008 80-84 22.660665166291576 28.3520880220055 27.921980495123783 21.065266316579145 85-89 23.625906476619154 28.012003000750184 27.286821705426355 21.0752688172043 90-94 23.20580145036259 27.47186796699175 28.382095523880967 20.940235058764692 95-99 23.670917729432357 27.67691922980745 28.092023005751436 20.560140035008754 100-104 24.461115278819705 27.561890472618156 27.56689172293073 20.41010252563141 105-109 23.485871467866968 27.89697424356089 27.80195048762191 20.81520380095024 110-114 24.20105026256564 27.431857964491122 27.991997999499873 20.37509377344336 115-119 24.79619904976244 28.35708927231808 26.93173293323331 19.91497874468617 120-124 25.311327831957993 28.052013003250813 27.22180545136284 19.414853713428357 125-129 25.626406601650416 27.671917979494875 26.971742935733932 19.72993248312078 130-134 25.698994648126845 27.58465462912019 27.194518081328468 19.5218326414245 135-139 26.577973392017608 27.573271981594477 27.123136941082326 18.725617685305593 140-144 26.716679169792446 26.731682920730183 27.62190547636909 18.929732433108278 145-149 27.89336801040312 26.90807242172652 26.377913374012202 18.820646193858156 150-151 27.43871935967984 27.66383191595798 25.962981490745374 18.934467233616807 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.0 12 0.5 13 0.5 14 0.5 15 1.0 16 1.0 17 2.0 18 2.5 19 1.0 20 0.0 21 0.5 22 0.5 23 3.5 24 4.0 25 2.0 26 3.5 27 6.0 28 10.5 29 13.5 30 16.0 31 27.0 32 40.5 33 49.5 34 63.5 35 64.5 36 80.5 37 114.5 38 129.0 39 144.0 40 190.5 41 220.0 42 235.0 43 272.0 44 272.0 45 253.5 46 247.5 47 239.5 48 235.5 49 204.0 50 167.5 51 144.0 52 115.0 53 107.0 54 83.5 55 54.5 56 38.5 57 29.0 58 28.5 59 17.0 60 11.0 61 10.0 62 6.5 63 6.0 64 2.5 65 0.0 66 1.0 67 2.0 68 1.0 69 0.0 70 0.5 71 2.0 72 2.0 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 0.0 84 0.5 85 0.5 86 0.0 87 0.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.5 97 0.5 98 0.5 99 0.5 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.375 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.025 25-29 0.025 30-34 0.025 35-39 0.025 40-44 0.025 45-49 0.025 50-54 0.025 55-59 0.025 60-64 0.025 65-69 0.02 70-74 0.025 75-79 0.025 80-84 0.025 85-89 0.025 90-94 0.025 95-99 0.025 100-104 0.025 105-109 0.025 110-114 0.025 115-119 0.025 120-124 0.025 125-129 0.025 130-134 0.034999999999999996 135-139 0.03 140-144 0.025 145-149 0.03 150-151 0.05 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.4 #Duplication Level Percentage of deduplicated Percentage of total 1 68.82530120481928 45.7 2 19.992469879518072 26.55 3 6.325301204819277 12.6 4 2.9367469879518073 7.8 5 1.1295180722891567 3.75 6 0.5271084337349398 2.1 7 0.11295180722891565 0.525 8 0.07530120481927711 0.4 9 0.0 0.0 >10 0.07530120481927711 0.575 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 13 0.325 No Hit AGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATC 10 0.25 No Hit AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 8 0.2 No Hit GGTTGGAGTTTCTTTGGGAAATGTTTCAAGAGTTTGAGATTTTTGAAAAA 8 0.2 No Hit CCCAAACAAAAAGTTTCTCTCCGTCTAGGGTTCCTGATTTACCGGACGTA 7 0.17500000000000002 No Hit GGGAGTTTGGGGAATTTGACCAGTTTAACTGGTCTTTATTTGCATGATAA 7 0.17500000000000002 No Hit AAGACTGCTGGTGTTTTTGTTCTTGTTGGGTTCCCAAGTGAAGTCAAATT 7 0.17500000000000002 No Hit GCGACACCAAAACAATTTTCTCACCTGCCAGTTTTGCAAAACACCTAGCT 6 0.15 No Hit TGTTAAACCAGGGCAAACCAGTGATCCTAGAAGTGGCTTCGCAACTGTTG 6 0.15 No Hit GTTACGTGTGCTCTGTCATGTGGACTCTGGCTTAATTTAGCTGACTTAGA 6 0.15 No Hit GTTATTATTGCAGAAGATTGTTTCCAGCTTTCAAACATTCCAAATGATCG 6 0.15 No Hit GGGGAGAGAATTGGGGAGAGAATGGGTATTACAAGATCTGCAGGGCTCGC 6 0.15 No Hit GTGGGTTCCTTGCTTGGAATTCGAGTTGGAGAAAGGTTGGGTCTACCGTG 6 0.15 No Hit GGGAGGCTGGATTTGATGAAATTGCAAGATTCATTTTGTACAAAGATTAA 6 0.15 No Hit GCAGTTTTGTTTGTGCATAGAGTGGTTGGGTTGCCTAAAGGGTTGTATTT 6 0.15 No Hit GTTTGTTAGAATGATTCTTCATTCTGGAAAATATGAAAGTGGAGATAGAT 6 0.15 No Hit GCCTGTTCCGAAGGGTATTGTATATGGTAAGCCCACAAACCAGGGTGTTA 6 0.15 No Hit GGATTTCTGGTTTCTGAAAGAGGCTGGCCTGGAGGAGAAACTTAATCTCT 6 0.15 No Hit GCTAGAAAGTGGAAAGAAGTCTGCTCCTTGAGATTGCTCAATACTTGTAG 6 0.15 No Hit GGTCCCTGTTTTCCATATCCAAGATAGACTCCACCTCCTGACTGTTGTCA 6 0.15 No Hit GGAAAATCAATTGGATGAAGGCTGGTATATTGGAAGCACACAGAGTTTTG 6 0.15 No Hit GCCAAAAACGTTCTGTAAGACCACGGTTGTATCTTTCTATTGACTCAGAC 5 0.125 No Hit AGATCTTTTTACTCGCCAGATCTCCTCCCCTGAAGTTGATTGGAGCTACG 5 0.125 No Hit GTTCAATTCTATATCTGGGATGCGAGTTGATGGATTTCACCTTCCAGAAG 5 0.125 No Hit CTTGCTCAGCATTGTGGCTGGTGGAGTTGTGCTTGTTGCCATTATTGGTG 5 0.125 No Hit GAGACAGAGATCGTTCCTACCCTTATAAAGTCATTGAAATTACACCTCCT 5 0.125 No Hit GAAGAATTTGATGTATTTGGCTGCAATTGCTGATGCACAACCACAGACAC 5 0.125 No Hit CTTGGGTACTCGAACTGGTCATAAGAAGAGGTTGCCTAAGACAAGTGATC 5 0.125 No Hit CTCGAGGGTCCAGACCGCATTTTTGTGGGTGGACTCCCTTATTACTTCAC 5 0.125 No Hit CGTCTCTTTCTCGAAAACAGCTAGTCTCTGCCCTCTTCACGGTGTACGTT 5 0.125 No Hit GGCGCACAGACAACATGGCTAGCTGCAACATGGCATCAGCTGCATCAGGA 5 0.125 No Hit AGGAGACCTTGGCAGTATCATCTCAAGTGCGAAGTTTGATGAGATGCTTA 5 0.125 No Hit GTGGTCGTTCTGTAGATCATGATAATGATTTTAATTCGTGTTGTTCTTCG 5 0.125 No Hit CTTCAATCACCTTCCCTTCTTGTTTTGCTTCAGCCAAGTTAGGTTTAGCA 5 0.125 No Hit GCAAGAGTTCCAAAAGTACAGACTGGGAGCTGCCCAAAATTGCTCTGCAA 5 0.125 No Hit GCCGACAAGGAGAAAACAGAAGCCTATATTCTTGAACAGGTATTGTGGCT 5 0.125 No Hit AATGGAGAGATAGATATGCTTCATAACCCTGGCTTTAGATATGGCTATCA 5 0.125 No Hit GGCCACTATTAGCAGCTCTTCTTTAAATTGAAGAAAAAACGACGTCGGTG 5 0.125 No Hit TCTTCCTTCGACAAGCCCCTTCGCCCCGCTCTCTTCAAAGCCACCTCCCT 5 0.125 No Hit GAGGGAACTGAGGGAAGAAACAGGAGTTACTTCAGCTGAATTTGTTGCAG 5 0.125 No Hit GTTTTTTTCCAGATAGCCAATTGGGTCGTTCGGTCTTTTATCTCTCGTGA 5 0.125 No Hit GAAGTCTCTGGTTGCTTCTTTGGCTTCTGGAATCGATTGTTCTCGAACCT 5 0.125 No Hit GGGCGTCTTATTCTGTGGCTCATAGGCTTGTTTGGGGTTGTATCGGTTGC 5 0.125 No Hit AAACAAGAGCGCGGTGGATAGGAGGAGAGCATAACCATTTTAGTCACATA 5 0.125 No Hit AGAAACCAATTGTTGCTGTTAATCATTGTGTTGCTCATATTGAGATGGGT 5 0.125 No Hit GACCCCCGAAGCTGTAACAGCCGCCTTTCCTAGTATAGCTGTCAGCGAGT 5 0.125 No Hit GAATTTTAAAGAGAGAGGCTTGTAATTTTAGTAAAATTAGGTGTTTTAGT 5 0.125 No Hit ACGTTCCCAAAGCAAAACCAAAAATGGCTACATGGTCTCTGCTCTCTCCT 5 0.125 No Hit GTTTGGTGTTCCAATGGGGTATGGTGGTCCTCATGCTGCGTTTTTGGCAA 5 0.125 No Hit CAAAAGACCACTACTCTTCCTCTCTATAATTTTCTAGGGTTTAGCAATGT 5 0.125 No Hit GTTTTATTCTGGATGGAATTCCTCGTTCACGGTTACAAGCTGAGATTCTT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0875 0.0 0.0 0.0 0.0 68-69 0.1 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.1625 0.0 0.0 0.0 0.0 74-75 0.23750000000000002 0.0 0.0 0.0 0.0 76-77 0.2875 0.0 0.0 0.0 0.0 78-79 0.5 0.0 0.0 0.0 0.0 80-81 0.625 0.0 0.0 0.0 0.0 82-83 0.875 0.0 0.0 0.0 0.0 84-85 1.1375 0.0 0.0 0.0 0.0 86-87 1.25 0.0 0.0 0.0 0.0 88-89 1.4249999999999998 0.0 0.0 0.0 0.0 90-91 1.675 0.0 0.0 0.0 0.0 92-93 1.7999999999999998 0.0 0.0 0.0 0.0 94-95 1.9375 0.0 0.0 0.0 0.0 96-97 2.025 0.0 0.0 0.0 0.0 98-99 2.2 0.0 0.0 0.0 0.0 100-101 2.55 0.0 0.0 0.0 0.0 102-103 2.9124999999999996 0.0 0.0 0.0 0.0 104-105 3.2125 0.0 0.0 0.0 0.0 106-107 3.4625 0.0 0.0 0.0 0.0 108-109 3.9124999999999996 0.0 0.0 0.0 0.0 110-111 4.725 0.0 0.0 0.0 0.0 112-113 5.125 0.0 0.0 0.0 0.0 114-115 5.725 0.0 0.0 0.0 0.0 116-117 6.2125 0.0 0.0 0.0 0.0 118-119 6.637499999999999 0.0 0.0 0.0 0.0 120-121 6.9375 0.0 0.0 0.0 0.0 122-123 7.375 0.0 0.0 0.0 0.0 124-125 7.975 0.0 0.0 0.0 0.0 126-127 8.6125 0.0 0.0 0.0 0.0 128-129 9.2625 0.0 0.0 0.0 0.0 130-131 10.125 0.0 0.0 0.0 0.0 132-133 10.6625 0.0 0.0 0.0 0.0 134-135 11.4125 0.0 0.0 0.0 0.0 136-137 11.962499999999999 0.0 0.0 0.0 0.0 138-139 12.55 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATCCCCT 10 0.006830828 145.0 6 TTACTCG 10 0.006830828 145.0 9 CATCCCC 10 0.006830828 145.0 5 ACTTCTA 10 0.006830828 145.0 145 CTGGTGT 10 0.006830828 145.0 8 GCTAATA 10 0.006830828 145.0 145 TTCCATC 10 0.006830828 145.0 2 CCATCCC 10 0.006830828 145.0 4 TCCATCC 10 0.006830828 145.0 3 GACTGCT 10 0.006830828 145.0 3 CCCCTCT 10 0.006830828 145.0 8 CCCTCTT 10 0.006830828 145.0 9 TCCCCTC 10 0.006830828 145.0 7 AAAAAAA 35 0.0035366106 20.714287 130-134 >>END_MODULE Read 1139191 spots for SRR13695445.sra Written 1139191 spots for SRR13695445.sra Read 1139191 spots for SRR13695445.sra Written 1139191 spots for SRR13695445.sra Read 1139191 spots for SRR13695445.sra Written 1139191 spots for SRR13695445.sra Read 1139191 spots for SRR13695445.sra Written 1139191 spots for SRR13695445.sra Read 1139191 spots for SRR13695445.sra Written 1139191 spots for SRR13695445.sra Read 1139191 spots for SRR13695445.sra Written 1139191 spots for SRR13695445.sra Read 1139191 spots for SRR13695445.sra Written 1139191 spots for SRR13695445.sra Read 1139191 spots for SRR13695445.sra Written 1139191 spots for SRR13695445.sra Read 1139191 spots for SRR13695445.sra Written 1139191 spots for SRR13695445.sra Read 1139205 spots for SRR13695445.sra Written 1139205 spots for SRR13695445.sra Read 1139191 spots for SRR13695445.sra Written 1139191 spots for SRR13695445.sra Read 1139191 spots for SRR13695445.sra Written 1139191 spots for SRR13695445.sra Read 1139191 spots for SRR13695445.sra Written 1139191 spots for SRR13695445.sra Read 1139191 spots for SRR13695445.sra Written 1139191 spots for SRR13695445.sra Read 1139191 spots for SRR13695445.sra Written 1139191 spots for SRR13695445.sra Read 1139191 spots for SRR13695445.sra Written 1139191 spots for SRR13695445.sra Read 1139191 spots for SRR13695445.sra Written 1139191 spots for SRR13695445.sra Read 1139191 spots for SRR13695445.sra Written 1139191 spots for SRR13695445.sra Read 1139191 spots for SRR13695445.sra Written 1139191 spots for SRR13695445.sra Read 1139191 spots for SRR13695445.sra Written 1139191 spots for SRR13695445.sra SRR ids: ['SRR13695445.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_jed9fsm8 SRR13695445.sra spots: 22783834 blocks: [[1, 1139191], [1139192, 2278382], [2278383, 3417573], [3417574, 4556764], [4556765, 5695955], [5695956, 6835146], [6835147, 7974337], [7974338, 9113528], [9113529, 10252719], [10252720, 11391910], [11391911, 12531101], [12531102, 13670292], [13670293, 14809483], [14809484, 15948674], [15948675, 17087865], [17087866, 18227056], [18227057, 19366247], [19366248, 20505438], [20505439, 21644629], [21644630, 22783834]] SRR13695445 file size 7721243 SRR13695445 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695445 SRR13695445_1.fastq SRR13695445_2.fastq Input file: SRR13695445_1.fastq Paired file: SRR13695445_2.fastq trimmed: SRR13695445-trimmed-pair1.fastq, SRR13695445-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 03:09:30 2025 >> started Wed Feb 12 03:09:56 2025 >> done (25.705s) 22783834 read pairs processed; of these: 247 ( 0.00%) short read pairs filtered out after trimming by size control 2111 ( 0.01%) empty read pairs filtered out after trimming by size control 22781476 (99.99%) read pairs available; of these: 3489345 (15.32%) trimmed read pairs available after processing 19292131 (84.68%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 6 0.00% 19 16 0.00% 20 18 0.00% 21 21 0.00% 22 17 0.00% 23 12 0.00% 24 19 0.00% 25 14 0.00% 26 17 0.00% 27 12 0.00% 28 27 0.00% 29 21 0.00% 30 22 0.00% 31 20 0.00% 32 20 0.00% 33 10 0.00% 34 31 0.00% 35 20 0.00% 36 27 0.00% 37 22 0.00% 38 36 0.00% 39 42 0.00% 40 31 0.00% 41 50 0.00% 42 33 0.00% 43 33 0.00% 44 60 0.00% 45 77 0.00% 46 74 0.00% 47 102 0.00% 48 122 0.00% 49 154 0.00% 50 141 0.00% 51 203 0.00% 52 224 0.00% 53 217 0.00% 54 278 0.00% 55 328 0.00% 56 398 0.00% 57 511 0.00% 58 520 0.00% 59 650 0.00% 60 853 0.00% 61 968 0.00% 62 1115 0.00% 63 1286 0.01% 64 1453 0.01% 65 1628 0.01% 66 2020 0.01% 67 2252 0.01% 68 2499 0.01% 69 2947 0.01% 70 3496 0.02% 71 4121 0.02% 72 4943 0.02% 73 5353 0.02% 74 5835 0.03% 75 6210 0.03% 76 6797 0.03% 77 7449 0.03% 78 7996 0.04% 79 9030 0.04% 80 9944 0.04% 81 10989 0.05% 82 12238 0.05% 83 13327 0.06% 84 14938 0.07% 85 15257 0.07% 86 15961 0.07% 87 16634 0.07% 88 17313 0.08% 89 18925 0.08% 90 19747 0.09% 91 21103 0.09% 92 22817 0.10% 93 24468 0.11% 94 25389 0.11% 95 26514 0.12% 96 27601 0.12% 97 28366 0.12% 98 28481 0.13% 99 30299 0.13% 100 31819 0.14% 101 32857 0.14% 102 35312 0.16% 103 35909 0.16% 104 37863 0.17% 105 38589 0.17% 106 39561 0.17% 107 40238 0.18% 108 40266 0.18% 109 41877 0.18% 110 41824 0.18% 111 44213 0.19% 112 46648 0.20% 113 47238 0.21% 114 49027 0.22% 115 50145 0.22% 116 50885 0.22% 117 52819 0.23% 118 52736 0.23% 119 52521 0.23% 120 53867 0.24% 121 55563 0.24% 122 56516 0.25% 123 59356 0.26% 124 61508 0.27% 125 60869 0.27% 126 63445 0.28% 127 62756 0.28% 128 63868 0.28% 129 63753 0.28% 130 64233 0.28% 131 64880 0.28% 132 66750 0.29% 133 68689 0.30% 134 70045 0.31% 135 71638 0.31% 136 71602 0.31% 137 71175 0.31% 138 71539 0.31% 139 73317 0.32% 140 72072 0.32% 141 72987 0.32% 142 73886 0.32% 143 75836 0.33% 144 77244 0.34% 145 78328 0.34% 146 78168 0.34% 147 80308 0.35% 148 79140 0.35% 149 79922 0.35% 150 80520 0.35% 151 19292131 84.68% 22781476 reads passed initial QC criterion=sequence-density sequence-density=0.54 sequence-density-rank=1 fanout-score=2.13 fanout-score-rank=28 prefix-density=0.57 prefix-fanout=2.0 sequence=TTCCACATAGTCCAGTAGCGTCCATCATAGTACCCTGGTGAGCTGTGGTGCTCACGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTTCGAGCGAAGAAGGTAATCAATTTCCTTGGCCAATTCCTCCTCTGTGAGATCTGGAAGGTAAGAAAGAGTCTCGAACTTCTTCAATCCAGTTGGTGGCCACACCTGCATGCATTGAACTCGTCCACCATTGCTTGCAATGGAAGTAATGTCATTAGCCTTTCTGGTACTGACTGGGAAAGCTGCGGCAGACTTGAGACCATTGAATGGTGCCACCAT criterion=fanout-score sequence-density=0.02 sequence-density-rank=32 fanout-score=270.60 fanout-score-rank=1 prefix-density=0.27 prefix-fanout=16.7 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTC criterion=sequence-density sequence-density=0.52 sequence-density-rank=1 fanout-score=2.99 fanout-score-rank=22 prefix-density=0.59 prefix-fanout=2.6 sequence=ATGGTGGCACCATTCAATGGTCTCAAGTCTGCCGCAGCTTTCCCAGTCAGTACCAGAAAGGC criterion=fanout-score sequence-density=0.12 sequence-density-rank=28 fanout-score=37.93 fanout-score-rank=1 prefix-density=0.34 prefix-fanout=12.9 sequence=AAAGAAAAGAAAA SRR13695445 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 03:10:37 Started mapping on | Feb 12 03:10:37 Finished on | Feb 12 03:12:57 Mapping speed, Million of reads per hour | 585.81 Number of input reads | 22781476 Average input read length | 293 UNIQUE READS: Uniquely mapped reads number | 20915958 Uniquely mapped reads % | 91.81% Average mapped length | 291.22 Number of splices: Total | 19635009 Number of splices: Annotated (sjdb) | 19211902 Number of splices: GT/AG | 19229302 Number of splices: GC/AG | 318738 Number of splices: AT/AC | 10967 Number of splices: Non-canonical | 76002 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.03% Deletion average length | 2.92 Insertion rate per base | 0.02% Insertion average length | 2.32 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 538503 % of reads mapped to multiple loci | 2.36% Number of reads mapped to too many loci | 105270 % of reads mapped to too many loci | 0.46% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 5.18% % of reads unmapped: other | 0.19% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1327305 1327305 1327305 N_multimapping 538503 538503 538503 N_noFeature 861356 20366018 1217573 N_ambiguous 316566 2482 120999 UnstrandedReadsAssigned:19738036 PositiveStrandReadsAssigned:547458 NegativeStrandReadsAssigned:19577386 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695445 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695445-trimmed-pair1.fastq SRR13695445-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 22,781,476 reads, 19,707,167 reads pseudoaligned [quant] estimated average fragment length: 239.513 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,177 rounds 52401 SRR13695445.ke.tsv 34699 SRR13695445.se.tsv 87100 total ==> SRR13695445.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1779.49 733 20.4003 Potri.005G024800.1.v4.1 1035 796.487 315 19.5866 Potri.004G059700.1.v4.1 961 722.625 0 0 Potri.007G009000.2.v4.1 1416 1177.49 0 0 Potri.003G141000.2.v4.1 2943 2704.49 1213.49 22.2217 Potri.016G087400.1.v4.1 270 94.0838 737.58 388.26 Potri.015G069301.1.v4.1 564 336.074 0 0 Potri.010G195200.1.v4.1 1773 1534.49 241 7.77825 Potri.012G127500.1.v4.1 977 738.562 110 7.37622 ==> SRR13695445.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 332 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 269 Potri.001G212900.v4.1 1 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 21 Potri.001G416900.v4.1 1 Potri.001G452600.v4.1 20 SRR13695445 completed mapping pipeline successfully