Starting /dee2/code/volunteer_pipeline.sh SRR13695446 current disk space = 3048989224960 free memory = 1559231292 SRR13695446 SRAfilesize 2dde37a389f7a9f81b106fc32692e659 SRR13695446.sra SRR13695446.sra file validated SRR13695446 is paired end SRR13695446 is conventional basespace SRR13695446 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695446_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.629 37.0 37.0 37.0 37.0 37.0 2 36.33025 37.0 37.0 37.0 37.0 37.0 3 36.5975 37.0 37.0 37.0 37.0 37.0 4 36.623 37.0 37.0 37.0 37.0 37.0 5 36.572 37.0 37.0 37.0 37.0 37.0 6 36.562 37.0 37.0 37.0 37.0 37.0 7 36.461 37.0 37.0 37.0 37.0 37.0 8 36.571 37.0 37.0 37.0 37.0 37.0 9 36.5505 37.0 37.0 37.0 37.0 37.0 10-14 36.579499999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.5381 37.0 37.0 37.0 37.0 37.0 20-24 36.5291 37.0 37.0 37.0 37.0 37.0 25-29 36.461499999999994 37.0 37.0 37.0 37.0 37.0 30-34 36.4528 37.0 37.0 37.0 37.0 37.0 35-39 36.424400000000006 37.0 37.0 37.0 37.0 37.0 40-44 36.419799999999995 37.0 37.0 37.0 37.0 37.0 45-49 36.367599999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.4037 37.0 37.0 37.0 37.0 37.0 55-59 36.41850000000001 37.0 37.0 37.0 37.0 37.0 60-64 36.365899999999996 37.0 37.0 37.0 37.0 37.0 65-69 36.3551 37.0 37.0 37.0 37.0 37.0 70-74 36.3783 37.0 37.0 37.0 37.0 37.0 75-79 36.3072 37.0 37.0 37.0 37.0 37.0 80-84 36.253 37.0 37.0 37.0 37.0 37.0 85-89 36.2301 37.0 37.0 37.0 37.0 37.0 90-94 36.1545 37.0 37.0 37.0 37.0 37.0 95-99 36.17530000000001 37.0 37.0 37.0 37.0 37.0 100-104 36.1588 37.0 37.0 37.0 37.0 37.0 105-109 36.1366 37.0 37.0 37.0 37.0 37.0 110-114 36.1661 37.0 37.0 37.0 37.0 37.0 115-119 36.0833 37.0 37.0 37.0 37.0 37.0 120-124 36.0043 37.0 37.0 37.0 37.0 37.0 125-129 36.0054 37.0 37.0 37.0 37.0 37.0 130-134 35.9391 37.0 37.0 37.0 37.0 37.0 135-139 35.8335 37.0 37.0 37.0 37.0 37.0 140-144 35.7417 37.0 37.0 37.0 37.0 37.0 145-149 35.5285 37.0 37.0 37.0 37.0 37.0 150-151 35.366 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 1.0 23 1.0 24 2.0 25 3.0 26 2.0 27 10.0 28 12.0 29 16.0 30 31.0 31 29.0 32 41.0 33 69.0 34 136.0 35 341.0 36 2962.0 37 344.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.674999999999997 11.675 9.425 47.225 2 20.22104998744034 13.765385581512183 35.543833207736746 30.469731223310724 3 20.025000000000002 17.424999999999997 23.3 39.25 4 23.400000000000002 23.375 25.174999999999997 28.050000000000004 5 24.9 31.2 22.85 21.05 6 20.0 35.3 24.725 19.975 7 15.8 26.775 39.574999999999996 17.849999999999998 8 19.35 25.775 30.4 24.474999999999998 9 16.55 23.925 35.35 24.175 10-14 19.405 29.265 27.275 24.055 15-19 20.169999999999998 27.544999999999998 28.285 24.0 20-24 20.36 28.87 28.144999999999996 22.625 25-29 20.285 28.12 28.189999999999998 23.405 30-34 20.125 28.53 27.529999999999998 23.815 35-39 20.18 27.305 28.04 24.474999999999998 40-44 19.99 27.97 28.360000000000003 23.68 45-49 20.41 28.000000000000004 27.634999999999998 23.955000000000002 50-54 19.685 28.985 27.66 23.669999999999998 55-59 20.54 28.87 27.505000000000003 23.085 60-64 20.165 28.76 27.105 23.97 65-69 20.205000000000002 28.82 27.37 23.605 70-74 20.395 29.14 27.029999999999998 23.435 75-79 20.435 28.88 27.3 23.385 80-84 20.95 28.335 27.134999999999998 23.580000000000002 85-89 20.3 28.24 27.589999999999996 23.87 90-94 20.13 28.57 27.694999999999997 23.605 95-99 20.169999999999998 28.355000000000004 28.005000000000003 23.47 100-104 20.745 28.249999999999996 27.42 23.585 105-109 19.43 29.110000000000003 28.244999999999997 23.215 110-114 20.46 28.34 27.529999999999998 23.669999999999998 115-119 20.585 28.939999999999998 27.04 23.435 120-124 20.424999999999997 28.470000000000002 26.88 24.224999999999998 125-129 20.674999999999997 28.275 26.915 24.135 130-134 21.375 28.144999999999996 27.095000000000002 23.385 135-139 20.8 28.76 26.55 23.89 140-144 20.68 28.599999999999998 26.919999999999998 23.799999999999997 145-149 21.52 28.199999999999996 25.945 24.335 150-151 21.075 26.900000000000002 27.925 24.099999999999998 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 0.5 22 0.5 23 1.0 24 1.0 25 2.0 26 4.5 27 5.5 28 8.0 29 13.5 30 19.5 31 20.5 32 25.5 33 38.0 34 51.0 35 67.0 36 80.0 37 106.0 38 138.5 39 167.0 40 201.5 41 210.0 42 194.5 43 241.0 44 283.5 45 276.0 46 261.0 47 270.0 48 265.5 49 209.5 50 165.0 51 149.0 52 129.5 53 86.5 54 68.0 55 63.0 56 46.5 57 33.0 58 23.5 59 20.0 60 20.0 61 12.0 62 7.5 63 5.5 64 2.0 65 2.0 66 2.0 67 0.5 68 0.0 69 1.0 70 1.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.475 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.075 #Duplication Level Percentage of deduplicated Percentage of total 1 71.88726364609347 50.375 2 18.65858009275776 26.150000000000002 3 6.386014983945773 13.425 4 1.6767748840528007 4.7 5 1.0346057795219408 3.6249999999999996 6 0.17838030681412773 0.75 7 0.10702818408847663 0.525 8 0.0 0.0 9 0.07135212272565108 0.44999999999999996 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCTCCCCAAACACTCTGTTGCTGTTGCAGTTGCTGCTGCATCATTTGCT 9 0.22499999999999998 No Hit ATCCAGGTCGATCTGCTGTCTCGATGCAGAGCAAGCTCCTCTTAGGTCCG 9 0.22499999999999998 No Hit CCATTTCATGGCTGCTTTAAGCGAATACATGGCATGTGCTGTCTTGTGTA 7 0.17500000000000002 No Hit CCCTTGCTTCCCATCCTCCAAATACTTCTTCCTATCAGCCTCATTCTCAT 7 0.17500000000000002 No Hit TCGTACTAGGGAAAGGTCCTCTCAATGCTCTAACGCCCACACCGGATATG 7 0.17500000000000002 No Hit GTAGAGAGTCATCATCACATTATAGGGAAGTGCATGCGTGACATAATCTT 6 0.15 No Hit CCGTTAACCTCTTCTTCTTGAGATACCTTGTTGTTGAAGGCCTTGTGAAC 6 0.15 No Hit CGCTGGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAG 6 0.15 No Hit CTCTTCTCCAACTCAGTCTCAACCATTTGAATAAGCATTTTCTCTGTCTC 6 0.15 No Hit GAGCAATCATAATAGGCATTGATATTTTGAATCCCAAGACAGTGGTGGCC 6 0.15 No Hit CGGTCCCCACTGCCAAATCTCTTGAACTTCCCATCATTGCATAAACCAAT 5 0.125 No Hit CATGGAAATTCAATATGGGGGTGATGCACCCCACAAGATCTTTTCGAAAA 5 0.125 No Hit AGTCCAAGATCGAACAAATACAAAAGCAAGAAGTGCAGCAGAGACATTAA 5 0.125 No Hit CCTTGTAACATCCAGCATCACCGCCATTCCTCTTAGCCTCCACAACAAAC 5 0.125 No Hit ACAGCCATGAATTTTGTTGCTTGCAATAAACTTGGCAGTGCCAATAGGTA 5 0.125 No Hit ACGTAGGATAAGCAACACAGGTGAGAACCCAGCCTTCCTCTATCTGGTCA 5 0.125 No Hit CTGGTTCTCTCTCCGAACCTCCTCCTCTTCCTCTGGTGTAAAGTCATTCT 5 0.125 No Hit TGTCGGTATTATACACTATCTACAGCTACTACAGATCCAATTCCAAAATG 5 0.125 No Hit CGCTGCTTTCATTTGAAATTATTTTCTGAGGATCAGTTTCGAGACGTTGA 5 0.125 No Hit ACTACTTGCTGCTGCTGTAGAGGAAGATGAAGATGTAGATGCTACTGTGG 5 0.125 No Hit GTTCGGAGTATGTTCATTGTTAGTCTGAATTCCTTCTCAAGACACTCAGC 5 0.125 No Hit CTTGGAACCTAGACACCCTTCGGCTTGGAGGCGATAAAACTGATGCACTG 5 0.125 No Hit AGCGATAGTTTTTTCAAATTGTTGTTCATCTGCAGGACTTCCAGGCTTGA 5 0.125 No Hit CTCAGGATCAGCTTCAATCATCTTCTGATAATAAACATCAGTACTCTCAA 5 0.125 No Hit CTTTGACATGGATTGAGTGTGTTGCCTCTTTGACCATTACAATACTCATA 5 0.125 No Hit TATCGTGCTTTGGGGGTAGGACCCTTGAGTAGACGAGGAACGGTTTCGAG 5 0.125 No Hit TGGAAATAGTGGGAGTTCAACGACGTCGTCTGATTTTGGATGGTTTGTGT 5 0.125 No Hit CCAGGAAAATGTGTCAATGCTTGCTGTTTCATGCTTTCCTGTCAACCTTC 5 0.125 No Hit CTCCAGTTTCATGGTTAACACAAGTCATATTGATCCGAGAATGAAAGCGA 5 0.125 No Hit GTCGCGCGCTTCAGCGCCATCCATTTTCGGGGCTAGTTGATTCGGCAGGT 5 0.125 No Hit CAGCACTAGCCTTGACAGGGAAAGAAGAGGCAAAGAGGCGTCTTGAACAT 5 0.125 No Hit AGAAGGGTGCAGGTGAGGTTGCGGTCACCATAGACATTGTCGACACGACC 5 0.125 No Hit CCACAGTGTCCCCTTCAAACGAAAGTTTCCAGTCTTGTCGATACCCTTCC 5 0.125 No Hit GGAGAAATGGGAGAAAAAGAAGAGCTGAGAGGGGATCATTTCAAAGTAAT 5 0.125 No Hit CTCCAATTTAGCCAGTGCTTCTTGCAAGTCAGCCTCAATTTTCTTCCTTC 5 0.125 No Hit CAACAAAATTCAAAGACACAAGCACAATAAACTAGGATAGATTTTATTTT 5 0.125 No Hit TATCGAAACAGAAATTCTGCACAAAATCGCAGTAATGGATTGACAAGCTA 5 0.125 No Hit GGTGTATCTGACATTGTTGTCCATTCATTTTCCATTTATGCTGGCCATCC 5 0.125 No Hit GCCAATGTATTGGGGTATGGGACCATTAATATTGCAATTTCTCAGAACCA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.15 0.0 0.0 0.0 0.0 80-81 0.1875 0.0 0.0 0.0 0.0 82-83 0.3125 0.0 0.0 0.0 0.0 84-85 0.4375 0.0 0.0 0.0 0.0 86-87 0.5125 0.0 0.0 0.0 0.0 88-89 0.575 0.0 0.0 0.0 0.0 90-91 0.7375 0.0 0.0 0.0 0.0 92-93 0.875 0.0 0.0 0.0 0.0 94-95 1.0375 0.0 0.0 0.0 0.0 96-97 1.15 0.0 0.0 0.0 0.0 98-99 1.3125 0.0 0.0 0.0 0.0 100-101 1.4874999999999998 0.0 0.0 0.0 0.0 102-103 1.8250000000000002 0.0 0.0 0.0 0.0 104-105 2.025 0.0 0.0 0.0 0.0 106-107 2.3375 0.0 0.0 0.0 0.0 108-109 2.6 0.0 0.0 0.0 0.0 110-111 2.8625 0.0 0.0 0.0 0.0 112-113 3.2125000000000004 0.0 0.0 0.0 0.0 114-115 3.7125 0.0 0.0 0.0 0.0 116-117 4.05 0.0 0.0 0.0 0.0 118-119 4.575 0.0 0.0 0.0 0.0 120-121 5.125 0.0 0.0 0.0 0.0 122-123 5.5875 0.0 0.0 0.0 0.0 124-125 6.1 0.0 0.0 0.0 0.0 126-127 6.5375 0.0 0.0 0.0 0.0 128-129 7.0375 0.0 0.0 0.0 0.0 130-131 7.5375 0.0 0.0 0.0 0.0 132-133 8.274999999999999 0.0 0.0 0.0 0.0 134-135 8.787500000000001 0.0 0.0 0.0 0.0 136-137 9.575 0.0 0.0 0.0 0.0 138-139 10.3 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCCACA 10 0.006830828 145.0 8 CTCCACG 10 0.006830828 145.0 2 CCCACAT 10 0.006830828 145.0 9 CACTTCC 10 0.006830828 145.0 3 >>END_MODULE SRR13695446 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695446_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.16425 37.0 37.0 37.0 37.0 37.0 2 36.2305 37.0 37.0 37.0 37.0 37.0 3 36.2475 37.0 37.0 37.0 37.0 37.0 4 36.3245 37.0 37.0 37.0 37.0 37.0 5 36.401 37.0 37.0 37.0 37.0 37.0 6 36.246 37.0 37.0 37.0 37.0 37.0 7 36.2545 37.0 37.0 37.0 37.0 37.0 8 36.423 37.0 37.0 37.0 37.0 37.0 9 36.387 37.0 37.0 37.0 37.0 37.0 10-14 36.363600000000005 37.0 37.0 37.0 37.0 37.0 15-19 36.3125 37.0 37.0 37.0 37.0 37.0 20-24 36.242599999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.2614 37.0 37.0 37.0 37.0 37.0 30-34 36.239549999999994 37.0 37.0 37.0 37.0 37.0 35-39 36.17960000000001 37.0 37.0 37.0 37.0 37.0 40-44 36.192099999999996 37.0 37.0 37.0 37.0 37.0 45-49 36.20585 37.0 37.0 37.0 37.0 37.0 50-54 36.13075 37.0 37.0 37.0 37.0 37.0 55-59 36.092 37.0 37.0 37.0 37.0 37.0 60-64 36.1087 37.0 37.0 37.0 37.0 37.0 65-69 36.018600000000006 37.0 37.0 37.0 37.0 37.0 70-74 36.0161 37.0 37.0 37.0 37.0 37.0 75-79 35.97760000000001 37.0 37.0 37.0 37.0 37.0 80-84 36.03745 37.0 37.0 37.0 37.0 37.0 85-89 35.86505 37.0 37.0 37.0 37.0 37.0 90-94 35.853699999999996 37.0 37.0 37.0 37.0 37.0 95-99 35.86495 37.0 37.0 37.0 37.0 37.0 100-104 35.8537 37.0 37.0 37.0 37.0 37.0 105-109 35.846849999999996 37.0 37.0 37.0 37.0 37.0 110-114 35.823499999999996 37.0 37.0 37.0 37.0 37.0 115-119 35.727199999999996 37.0 37.0 37.0 37.0 37.0 120-124 35.6488 37.0 37.0 37.0 37.0 37.0 125-129 35.61255 37.0 37.0 37.0 37.0 37.0 130-134 35.43645 37.0 37.0 37.0 34.6 37.0 135-139 35.460150000000006 37.0 37.0 37.0 37.0 37.0 140-144 35.2374 37.0 37.0 37.0 32.2 37.0 145-149 35.221050000000005 37.0 37.0 37.0 32.2 37.0 150-151 34.834875 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.0 15 2.0 16 0.0 17 1.0 18 1.0 19 0.0 20 1.0 21 0.0 22 3.0 23 2.0 24 2.0 25 5.0 26 9.0 27 14.0 28 13.0 29 18.0 30 25.0 31 35.0 32 58.0 33 113.0 34 208.0 35 582.0 36 2711.0 37 195.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.69761606022585 18.619824341279802 15.48306148055207 33.199498117942284 2 26.5 24.325 32.1 17.075000000000003 3 21.125 28.025 31.45 19.400000000000002 4 22.650000000000002 33.775 24.05 19.525000000000002 5 24.0 37.075 22.55 16.375 6 21.05 37.9 22.45 18.6 7 20.150000000000002 22.075 39.0 18.775 8 21.75 24.875 29.275000000000002 24.099999999999998 9 21.65 24.125 31.724999999999998 22.5 10-14 22.68 29.515 27.315 20.49 15-19 22.545 28.62 27.584999999999997 21.25 20-24 21.9665899769931 28.958687606281885 27.908372511753527 21.16634990497149 25-29 22.88144072036018 28.54927463731866 27.378689344672335 21.190595297648823 30-34 23.318161356474768 27.099484819686893 28.379932976541788 21.202420847296555 35-39 22.361708512553765 27.87836350905272 28.698609582874862 21.061318395518654 40-44 22.26390556222489 28.216286514605844 29.096638655462186 20.423169267707085 45-49 22.067723703296156 28.16985945080778 29.0401640574201 20.722252788475966 50-54 22.520630157539383 28.617154288572145 27.576894223555886 21.285321330332582 55-59 22.991495747873934 28.199099549774886 27.973986993496748 20.83541770885443 60-64 23.592077623286986 27.363208962688805 27.81834550365109 21.226367910373114 65-69 23.16963392678536 27.065413082616523 29.010802160432085 20.754150830166033 70-74 22.726363181590795 28.244122061030513 28.139069534767387 20.890445222611305 75-79 22.894157663065226 27.901160464185676 28.456382553021207 20.74829931972789 80-84 23.270817704426104 28.657164291072768 27.38184546136534 20.690172543135784 85-89 23.94577559901956 28.127657445850634 26.792056425391426 21.13451052973838 90-94 23.1519455836751 29.018705611683504 27.773331999599883 20.056016805041512 95-99 23.945986496624155 27.991997999499873 27.73693423355839 20.32508127031758 100-104 24.45722861430715 28.419209604802404 27.01350675337669 20.110055027513756 105-109 23.650642789255162 27.582412085438445 27.30228602871292 21.464659096593465 110-114 23.957187156146844 28.573572071621488 27.088126437931383 20.38111433430029 115-119 23.504401760704283 28.091236494597837 27.66106442577031 20.74329731892757 120-124 24.912456228114056 28.194097048524263 26.74837418709355 20.145072536268135 125-129 25.06627982592167 27.982592166474912 26.77704967235256 20.174078335250865 130-134 25.281432931405412 27.93815980387252 27.037574423375194 19.742832841346875 135-139 25.78418129971484 28.330581820001 26.709690329681322 19.17554655060283 140-144 25.777733319996 27.813344003200964 26.89806942082625 19.510853255976794 145-149 25.899244584521487 28.400620341187654 26.664665566061334 19.03546950822953 150-151 27.423995996496934 27.12373326660828 27.223820843237835 18.22844989365695 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 0.5 13 0.5 14 0.5 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 1.0 22 0.5 23 0.0 24 0.5 25 3.0 26 9.0 27 12.5 28 10.0 29 10.5 30 22.5 31 28.5 32 32.5 33 39.0 34 58.5 35 86.0 36 97.0 37 127.0 38 155.5 39 159.5 40 193.5 41 214.5 42 229.0 43 279.0 44 296.0 45 275.5 46 265.0 47 244.5 48 215.5 49 189.5 50 142.0 51 111.5 52 108.0 53 84.5 54 67.0 55 59.0 56 36.0 57 20.5 58 22.0 59 29.5 60 17.0 61 6.0 62 8.5 63 8.5 64 5.5 65 3.0 66 3.0 67 2.0 68 3.0 69 3.0 70 0.0 71 0.0 72 0.5 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.375 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.03 25-29 0.05 30-34 0.034999999999999996 35-39 0.03 40-44 0.04 45-49 0.034999999999999996 50-54 0.025 55-59 0.05 60-64 0.03 65-69 0.02 70-74 0.05 75-79 0.04 80-84 0.025 85-89 0.045 90-94 0.03 95-99 0.025 100-104 0.05 105-109 0.045 110-114 0.03 115-119 0.04 120-124 0.05 125-129 0.045 130-134 0.065 135-139 0.055 140-144 0.03 145-149 0.055 150-151 0.08750000000000001 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.15 #Duplication Level Percentage of deduplicated Percentage of total 1 72.34497505345688 50.74999999999999 2 18.353528153955807 25.75 3 6.058446186742694 12.75 4 1.8175338560228083 5.1 5 0.962223806129722 3.375 6 0.24946543121881684 1.05 7 0.10691375623663579 0.525 8 0.03563791874554526 0.2 9 0.03563791874554526 0.22499999999999998 >10 0.03563791874554526 0.27499999999999997 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 11 0.27499999999999997 No Hit GCCCAAGTGGATCTTCACCACCAGCAACCTGGGATCTTCTGTATGCGGCT 9 0.22499999999999998 No Hit ATTTGGGATTGGATGTGGCAAAGGGAACTGTTCTCACTGATGGACCAGTC 8 0.2 No Hit GGATTAGTAGCGGTGATGGTGTGGGGTTTGTCACTAGTAATAATAATAGG 7 0.17500000000000002 No Hit GGCTGATCTTCCCCAAGAGCTCACATCGACGGGAAGGTTTGGCACCTCGA 7 0.17500000000000002 No Hit GAGGGTTTCCGCAAAATTACATTTTATCAGGATCGCCCTGATATAATGGC 7 0.17500000000000002 No Hit CGGCAAGGCTCGAACACCACGTCCGGTGATTACCATGACTATATTTCGAC 6 0.15 No Hit CTATTTTCTCCCTTTTGCTACAACCAGATCTCATCAACACCGACAGACAG 6 0.15 No Hit TGGAAAAAGAAACTTACAAATCAATCCCTGCAGCTTTTTGAATTTTTGCC 6 0.15 No Hit ATTAGACCTTATTGCCAAAGTGCGGGGAGTTTCTAGCGCTGAAGATTTTT 6 0.15 No Hit CCAAGATTGTTTAAGTGGGAGGATTTAACAGTTGATTTTAAAGATGGAGA 6 0.15 No Hit TAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGT 6 0.15 No Hit TTGATGAAGCTACTGCTTCCATTGATTCTGCTACTGATGCCATTCTACAA 6 0.15 No Hit GGCAAAACAAGAGGAGGAAACAAGAGGATATTTCGAGGGAATAGCTCCTA 5 0.125 No Hit TCATGGTTGAGTTCTTGGTCCTCATGGTTGCTTTGGACAAAATCTGGTTC 5 0.125 No Hit CGGGCTGATTACCTTGCAGCCAAGAAGGATATAATGGATGCATCTGGGGT 5 0.125 No Hit GTTAAATACTTGGGCCCGTTCTCTGGTGAGCCCCCATCCTACTTGACTGG 5 0.125 No Hit ACAAAGACCATGAGGGAGGAAGGAGGCTATGAAGCAATAAAGAAGGCAAT 5 0.125 No Hit GGGTCTGAATCTGTCTTCCCTTATTTTGATTTGGAGACCATAGTCCATGG 5 0.125 No Hit TGAGTACCTTCTTCGCAACAAGTGGGTTCCTTGCTTGGAATTCGAGTTGG 5 0.125 No Hit GAAACTGCCTGAGCTAGAGAAGCGTCTTATAAGCTTGAACTCTGGTGATG 5 0.125 No Hit CTTATATGTCAATCTTGATCTGTAAAGGTGTGGTGGGTGTGGCTGTAATT 5 0.125 No Hit CTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATAGATAGCCA 5 0.125 No Hit GAGATGGAGATGGAGATGGAGGTAGATCCACAAGATGAACCAGAAGGGTC 5 0.125 No Hit CCGGGTTACGGTGCCCAACTGCGCGCTAACCTAGAACCCACAAAGGGTGT 5 0.125 No Hit CTTGGACGTATTATCAATAGGTTTGCCAAGGATCTAGGTGATATTGATCG 5 0.125 No Hit TGCATGCATTGCTTTGACACCATTGTTCCATAGTTTTGATTGAAACTCTC 5 0.125 No Hit AGAATGGCCACCACAGCAGCCCTCTCCAGCGCCATGGTCAGCACATCGTT 5 0.125 No Hit GGAGCTTGGAAATTTAACCAAACTTCGAATCCTATACTTGACAGCGAATA 5 0.125 No Hit AGATATTGCAACAAAAGGCTGCTGAAGCTCTTGCAGAGCTAATTTCACGT 5 0.125 No Hit ACAGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTT 5 0.125 No Hit TTCAAATTCAAATCCAATGCCTTATTTTCACCAAATCCAAGGCGTAACTG 5 0.125 No Hit AGCGTCTTATGGACTATGGATTTCATGCACCTACAATGTCATGGCCAGTT 5 0.125 No Hit ATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAAT 5 0.125 No Hit GAGGAGTTCAACAAGGATGATGAGAGAAAAGACGAGGAATTGGCTTCTAC 5 0.125 No Hit GCACAGCATGCCGCGCTCAATTTTGGGCAGTACCCCTATGGTGGTTATGT 5 0.125 No Hit GTTCTTTGAAGGATGTGAGGTTGGTAGTGAAGGTGGTGGAGGGAAGTTTT 5 0.125 No Hit GCTTGGGTTAAAACAAATGTTCAAGCTTATTTACCAGCAACAAAAATCAC 5 0.125 No Hit ACTGAATCGAACCGAACTAAAGTGTCCGTCCCTCGACATTCTTCCCCAAA 5 0.125 No Hit CAACGCCGGCGATCTCCTCTCTCTTTATTATCTTCTCCACCCACCATCTC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.16249999999999998 0.0 0.0 0.0 0.0 80-81 0.21250000000000002 0.0 0.0 0.0 0.0 82-83 0.3375 0.0 0.0 0.0 0.0 84-85 0.4625 0.0 0.0 0.0 0.0 86-87 0.5625 0.0 0.0 0.0 0.0 88-89 0.625 0.0 0.0 0.0 0.0 90-91 0.7875 0.0 0.0 0.0 0.0 92-93 0.925 0.0 0.0 0.0 0.0 94-95 1.0875 0.0 0.0 0.0 0.0 96-97 1.2 0.0 0.0 0.0 0.0 98-99 1.375 0.0 0.0 0.0 0.0 100-101 1.55 0.0 0.0 0.0 0.0 102-103 1.875 0.0 0.0 0.0 0.0 104-105 2.075 0.0 0.0 0.0 0.0 106-107 2.3625 0.0 0.0 0.0 0.0 108-109 2.625 0.0 0.0 0.0 0.0 110-111 2.8875 0.0 0.0 0.0 0.0 112-113 3.225 0.0 0.0 0.0 0.0 114-115 3.7125 0.0 0.0 0.0 0.0 116-117 4.075 0.0 0.0 0.0 0.0 118-119 4.6 0.0 0.0 0.0 0.0 120-121 5.15 0.0 0.0 0.0 0.0 122-123 5.612500000000001 0.0 0.0 0.0 0.0 124-125 6.125 0.0 0.0 0.0 0.0 126-127 6.5625 0.0 0.0 0.0 0.0 128-129 7.0625 0.0 0.0 0.0 0.0 130-131 7.550000000000001 0.0 0.0 0.0 0.0 132-133 8.274999999999999 0.0 0.0 0.0 0.0 134-135 8.775 0.0 0.0 0.0 0.0 136-137 9.6 0.0 0.0 0.0 0.0 138-139 10.325 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTTTC 10 0.006830828 145.0 3 >>END_MODULE Read 826594 spots for SRR13695446.sra Written 826594 spots for SRR13695446.sra Read 826594 spots for SRR13695446.sra Read 826594 spots for SRR13695446.sra Written 826594 spots for SRR13695446.sra Written 826594 spots for SRR13695446.sra Read 826594 spots for SRR13695446.sra Written 826594 spots for SRR13695446.sra Read 826594 spots for SRR13695446.sra Written 826594 spots for SRR13695446.sra Read 826594 spots for SRR13695446.sra Written 826594 spots for SRR13695446.sra Read 826594 spots for SRR13695446.sra Written 826594 spots for SRR13695446.sra Read 826594 spots for SRR13695446.sra Written 826594 spots for SRR13695446.sra Read 826603 spots for SRR13695446.sra Written 826603 spots for SRR13695446.sra Read 826594 spots for SRR13695446.sra Written 826594 spots for SRR13695446.sra Read 826594 spots for SRR13695446.sra Written 826594 spots for SRR13695446.sra Read 826594 spots for SRR13695446.sra Written 826594 spots for SRR13695446.sra Read 826594 spots for SRR13695446.sra Written 826594 spots for SRR13695446.sra Read 826594 spots for SRR13695446.sra Written 826594 spots for SRR13695446.sra Read 826594 spots for SRR13695446.sra Written 826594 spots for SRR13695446.sra Read 826594 spots for SRR13695446.sra Written 826594 spots for SRR13695446.sra Read 826594 spots for SRR13695446.sra Written 826594 spots for SRR13695446.sra Read 826594 spots for SRR13695446.sra Written 826594 spots for SRR13695446.sra Read 826594 spots for SRR13695446.sra Written 826594 spots for SRR13695446.sra Read 826594 spots for SRR13695446.sra Written 826594 spots for SRR13695446.sra SRR ids: ['SRR13695446.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_u4l7mxhz SRR13695446.sra spots: 16531889 blocks: [[1, 826594], [826595, 1653188], [1653189, 2479782], [2479783, 3306376], [3306377, 4132970], [4132971, 4959564], [4959565, 5786158], [5786159, 6612752], [6612753, 7439346], [7439347, 8265940], [8265941, 9092534], [9092535, 9919128], [9919129, 10745722], [10745723, 11572316], [11572317, 12398910], [12398911, 13225504], [13225505, 14052098], [14052099, 14878692], [14878693, 15705286], [15705287, 16531889]] SRR13695446 file size 5596558 SRR13695446 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695446 SRR13695446_1.fastq SRR13695446_2.fastq Input file: SRR13695446_1.fastq Paired file: SRR13695446_2.fastq trimmed: SRR13695446-trimmed-pair1.fastq, SRR13695446-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 03:51:07 2025 >> started Wed Feb 12 03:51:25 2025 >> done (18.344s) 16531889 read pairs processed; of these: 120 ( 0.00%) short read pairs filtered out after trimming by size control 5320 ( 0.03%) empty read pairs filtered out after trimming by size control 16526449 (99.97%) read pairs available; of these: 2357667 (14.27%) trimmed read pairs available after processing 14168782 (85.73%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 4 0.00% 19 0 0.00% 20 1 0.00% 21 1 0.00% 22 2 0.00% 23 1 0.00% 24 4 0.00% 25 1 0.00% 26 5 0.00% 27 3 0.00% 28 4 0.00% 29 12 0.00% 30 6 0.00% 31 12 0.00% 32 4 0.00% 33 7 0.00% 34 9 0.00% 35 14 0.00% 36 11 0.00% 37 12 0.00% 38 27 0.00% 39 20 0.00% 40 14 0.00% 41 36 0.00% 42 46 0.00% 43 34 0.00% 44 30 0.00% 45 51 0.00% 46 47 0.00% 47 45 0.00% 48 97 0.00% 49 107 0.00% 50 140 0.00% 51 127 0.00% 52 146 0.00% 53 184 0.00% 54 176 0.00% 55 220 0.00% 56 219 0.00% 57 244 0.00% 58 287 0.00% 59 324 0.00% 60 408 0.00% 61 495 0.00% 62 603 0.00% 63 639 0.00% 64 678 0.00% 65 716 0.00% 66 783 0.00% 67 950 0.01% 68 1049 0.01% 69 1208 0.01% 70 1425 0.01% 71 1699 0.01% 72 1845 0.01% 73 2141 0.01% 74 2378 0.01% 75 2694 0.02% 76 2912 0.02% 77 3167 0.02% 78 3550 0.02% 79 3867 0.02% 80 4464 0.03% 81 5023 0.03% 82 5350 0.03% 83 6031 0.04% 84 6709 0.04% 85 7350 0.04% 86 7974 0.05% 87 8505 0.05% 88 9260 0.06% 89 9604 0.06% 90 10547 0.06% 91 11335 0.07% 92 11880 0.07% 93 12944 0.08% 94 13997 0.08% 95 14929 0.09% 96 15879 0.10% 97 17100 0.10% 98 17451 0.11% 99 17871 0.11% 100 19270 0.12% 101 19525 0.12% 102 20478 0.12% 103 21483 0.13% 104 22295 0.13% 105 23465 0.14% 106 25261 0.15% 107 25838 0.16% 108 26940 0.16% 109 27434 0.17% 110 27990 0.17% 111 28693 0.17% 112 29468 0.18% 113 30460 0.18% 114 31573 0.19% 115 32926 0.20% 116 34534 0.21% 117 35497 0.21% 118 35965 0.22% 119 36123 0.22% 120 37475 0.23% 121 38911 0.24% 122 38350 0.23% 123 40062 0.24% 124 40750 0.25% 125 41147 0.25% 126 43476 0.26% 127 44223 0.27% 128 45309 0.27% 129 45512 0.28% 130 46940 0.28% 131 46866 0.28% 132 47231 0.29% 133 48617 0.29% 134 48259 0.29% 135 49770 0.30% 136 50959 0.31% 137 51863 0.31% 138 53038 0.32% 139 54537 0.33% 140 54381 0.33% 141 55383 0.34% 142 55946 0.34% 143 55957 0.34% 144 57158 0.35% 145 57450 0.35% 146 58863 0.36% 147 58957 0.36% 148 61546 0.37% 149 61611 0.37% 150 61758 0.37% 151 14168782 85.73% 16526449 reads passed initial QC criterion=sequence-density sequence-density=0.37 sequence-density-rank=1 fanout-score=1.98 fanout-score-rank=23 prefix-density=0.38 prefix-fanout=1.9 sequence=TGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGAGTATGAATGTGTTCTCTGTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=26 fanout-score=329.84 fanout-score-rank=1 prefix-density=0.26 prefix-fanout=18.0 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAG criterion=sequence-density sequence-density=1.06 sequence-density-rank=1 fanout-score=2.06 fanout-score-rank=28 prefix-density=1.06 prefix-fanout=2.0 sequence=CTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAGTGAACAAACAAAAGTGTCCGTGAGTGTCTTTATCTTTGTGTCTTCTTCA criterion=fanout-score sequence-density=0.01 sequence-density-rank=32 fanout-score=26.76 fanout-score-rank=1 prefix-density=0.09 prefix-fanout=2.1 sequence=CCACCACTAAACCCAAAGCTCAAAAAAAATCTTAACTGCACCCGCTCATCCAGCAATGGCAGCAGCAACAATGGCCCTCTCCTCCCCTTCGCTAGCCGGAAAGGCGGTGAAGCTCAACCCCTCCTCCTCTGAGATCATGGGCAATGGCCGTGTCTCCATGAGGAAAACCACCAAGCCTGTTCCCTCCGGGAGCCCATGGTACGGACCAGACCGTGTTAAATACTTGGGCCCGTTCTCTGGTGAGCCCCCATCCTACTTGACTGGTGAGTTCCCTGG SRR13695446 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 03:52:04 Started mapping on | Feb 12 03:52:04 Finished on | Feb 12 03:54:03 Mapping speed, Million of reads per hour | 499.96 Number of input reads | 16526449 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 15474209 Uniquely mapped reads % | 93.63% Average mapped length | 293.30 Number of splices: Total | 15302583 Number of splices: Annotated (sjdb) | 14953926 Number of splices: GT/AG | 14987410 Number of splices: GC/AG | 241736 Number of splices: AT/AC | 8808 Number of splices: Non-canonical | 64629 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.03% Deletion average length | 2.79 Insertion rate per base | 0.02% Insertion average length | 2.35 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 388843 % of reads mapped to multiple loci | 2.35% Number of reads mapped to too many loci | 101886 % of reads mapped to too many loci | 0.62% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.23% % of reads unmapped: other | 0.16% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 663573 663573 663573 N_multimapping 388843 388843 388843 N_noFeature 631353 15070927 882431 N_ambiguous 249363 1519 96087 UnstrandedReadsAssigned:14593493 PositiveStrandReadsAssigned:401763 NegativeStrandReadsAssigned:14495691 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695446 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695446-trimmed-pair1.fastq SRR13695446-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 16,526,449 reads, 14,531,016 reads pseudoaligned [quant] estimated average fragment length: 240.43 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,106 rounds 52401 SRR13695446.ke.tsv 34699 SRR13695446.se.tsv 87100 total ==> SRR13695446.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1778.57 444 15.5181 Potri.005G024800.1.v4.1 1035 795.57 261 20.3933 Potri.004G059700.1.v4.1 961 721.619 1 0.0861427 Potri.007G009000.2.v4.1 1416 1176.57 0 0 Potri.003G141000.2.v4.1 2943 2703.57 814.404 18.7253 Potri.016G087400.1.v4.1 270 89.0534 720 502.584 Potri.015G069301.1.v4.1 564 332.86 0 0 Potri.010G195200.1.v4.1 1773 1533.57 75 3.04007 Potri.012G127500.1.v4.1 977 737.589 83 6.99504 ==> SRR13695446.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 154 Potri.001G233950.v4.1 1 Potri.001G122700.v4.1 268 Potri.001G212900.v4.1 13 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 14 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 4 SRR13695446 completed mapping pipeline successfully