Starting /dee2/code/volunteer_pipeline.sh SRR13695447 current disk space = 3048998912000 free memory = 1320565592 SRR13695447 SRAfilesize 9815b50f2e25bf04d3863988edaedcfb SRR13695447.sra SRR13695447.sra file validated SRR13695447 is paired end SRR13695447 is conventional basespace SRR13695447 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695447_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5445 37.0 37.0 37.0 37.0 37.0 2 36.35575 37.0 37.0 37.0 37.0 37.0 3 36.457 37.0 37.0 37.0 37.0 37.0 4 36.5815 37.0 37.0 37.0 37.0 37.0 5 36.562 37.0 37.0 37.0 37.0 37.0 6 36.5175 37.0 37.0 37.0 37.0 37.0 7 36.4525 37.0 37.0 37.0 37.0 37.0 8 36.636 37.0 37.0 37.0 37.0 37.0 9 36.6305 37.0 37.0 37.0 37.0 37.0 10-14 36.547 37.0 37.0 37.0 37.0 37.0 15-19 36.5248 37.0 37.0 37.0 37.0 37.0 20-24 36.4754 37.0 37.0 37.0 37.0 37.0 25-29 36.4335 37.0 37.0 37.0 37.0 37.0 30-34 36.4446 37.0 37.0 37.0 37.0 37.0 35-39 36.4836 37.0 37.0 37.0 37.0 37.0 40-44 36.400400000000005 37.0 37.0 37.0 37.0 37.0 45-49 36.3897 37.0 37.0 37.0 37.0 37.0 50-54 36.3934 37.0 37.0 37.0 37.0 37.0 55-59 36.3483 37.0 37.0 37.0 37.0 37.0 60-64 36.3313 37.0 37.0 37.0 37.0 37.0 65-69 36.301500000000004 37.0 37.0 37.0 37.0 37.0 70-74 36.2741 37.0 37.0 37.0 37.0 37.0 75-79 36.25789999999999 37.0 37.0 37.0 37.0 37.0 80-84 36.1995 37.0 37.0 37.0 37.0 37.0 85-89 36.2273 37.0 37.0 37.0 37.0 37.0 90-94 36.1186 37.0 37.0 37.0 37.0 37.0 95-99 36.13440000000001 37.0 37.0 37.0 37.0 37.0 100-104 36.1635 37.0 37.0 37.0 37.0 37.0 105-109 36.124 37.0 37.0 37.0 37.0 37.0 110-114 36.0118 37.0 37.0 37.0 37.0 37.0 115-119 36.111599999999996 37.0 37.0 37.0 37.0 37.0 120-124 35.9646 37.0 37.0 37.0 37.0 37.0 125-129 35.98559999999999 37.0 37.0 37.0 37.0 37.0 130-134 35.937200000000004 37.0 37.0 37.0 37.0 37.0 135-139 35.8885 37.0 37.0 37.0 37.0 37.0 140-144 35.869899999999994 37.0 37.0 37.0 37.0 37.0 145-149 35.67 37.0 37.0 37.0 37.0 37.0 150-151 35.577 37.0 37.0 37.0 37.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 1.0 24 1.0 25 6.0 26 9.0 27 7.0 28 8.0 29 14.0 30 24.0 31 39.0 32 60.0 33 64.0 34 133.0 35 351.0 36 2921.0 37 362.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.2 10.625 14.099999999999998 45.074999999999996 2 20.382005529027396 13.847700427243026 34.53128926866047 31.239004775069112 3 19.7 15.950000000000001 24.975 39.375 4 21.125 24.55 23.425 30.9 5 21.3 33.375 25.5 19.825 6 22.925 34.075 23.549999999999997 19.45 7 15.475 25.8 40.8 17.925 8 20.65 24.5 31.525 23.325000000000003 9 19.025 23.9 34.125 22.95 10-14 19.580000000000002 29.95 27.245 23.225 15-19 19.665 28.57 27.915 23.849999999999998 20-24 20.895 28.389999999999997 27.045 23.669999999999998 25-29 21.475 28.265 28.01 22.25 30-34 19.465 28.345 27.810000000000002 24.38 35-39 19.415 29.325000000000003 28.244999999999997 23.015 40-44 19.45 29.285 27.96 23.305 45-49 20.51 27.655 27.325 24.51 50-54 20.34 27.785 28.449999999999996 23.425 55-59 19.965 28.560000000000002 28.175 23.3 60-64 20.119999999999997 27.944999999999997 27.994999999999997 23.94 65-69 20.11 28.415000000000003 28.235 23.24 70-74 20.275000000000002 28.605000000000004 27.13 23.990000000000002 75-79 19.950000000000003 27.195000000000004 28.595 24.26 80-84 20.585 28.37 27.805000000000003 23.24 85-89 20.03 29.145 27.339999999999996 23.485 90-94 21.01 27.950000000000003 27.375 23.665 95-99 19.965 28.395 27.500000000000004 24.14 100-104 20.575 28.4 27.694999999999997 23.330000000000002 105-109 20.26 28.235 27.779999999999998 23.724999999999998 110-114 20.305 29.445 27.42 22.830000000000002 115-119 20.57 28.449999999999996 26.595000000000002 24.385 120-124 21.07 28.32 26.795 23.815 125-129 20.064999999999998 29.445 27.92 22.57 130-134 20.810000000000002 29.28 26.290000000000003 23.62 135-139 21.044999999999998 27.534999999999997 27.405 24.015 140-144 20.865000000000002 27.36 28.205000000000002 23.57 145-149 21.375 27.310000000000002 27.04 24.275 150-151 20.075000000000003 27.625 27.9375 24.3625 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 0.5 22 0.0 23 0.0 24 0.5 25 0.5 26 1.0 27 7.0 28 12.5 29 12.5 30 11.0 31 24.5 32 29.5 33 29.5 34 52.5 35 79.0 36 86.0 37 107.5 38 147.5 39 191.0 40 206.0 41 214.0 42 231.5 43 252.0 44 248.5 45 228.0 46 282.5 47 298.5 48 246.5 49 204.5 50 160.5 51 142.0 52 108.0 53 68.0 54 69.5 55 56.0 56 39.5 57 32.5 58 33.5 59 27.5 60 15.0 61 11.5 62 7.5 63 8.0 64 5.0 65 2.0 66 3.5 67 2.0 68 0.5 69 0.0 70 0.0 71 0.0 72 0.5 73 1.5 74 1.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.525 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.925 #Duplication Level Percentage of deduplicated Percentage of total 1 66.64059444661713 42.6 2 19.78881501759875 25.3 3 8.017207665232695 15.375 4 3.1286664059444664 8.0 5 1.6034415330465388 5.125 6 0.4301916308173641 1.6500000000000001 7 0.23464998044583496 1.05 8 0.07821666014861164 0.4 9 0.03910833007430582 0.22499999999999998 >10 0.03910833007430582 0.27499999999999997 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGCCTGTGAAAGTATACCCAGCATATGCAACCATGTCTAACAAGGGTGCC 11 0.27499999999999997 No Hit CTAGAAAACACTGCATACGAGCTTAGCATTCAATATTCAAATTTGGTGTA 9 0.22499999999999998 No Hit CCAAGATCAGCTGACCTATCAAACATCTCCTTCATTCCAGGAATCAAAGT 8 0.2 No Hit CAGGAATGCCATCTCTTTCTCTGAGAACCTCAGGCCGGACATCAATGAGT 8 0.2 No Hit ATCAAACATCACTGCTTGTAGCCCAGTCAACTCAGATAGAAAAGATAAAT 7 0.17500000000000002 No Hit GCCATTCTAATTGCCATGGAAGCTCTAAGGAAATATACAAACTTTACTTA 7 0.17500000000000002 No Hit GTCAGCGCATAGAGTTGTCTGCAGACAGAACCGATTGATGCGATATCTCT 7 0.17500000000000002 No Hit GTCCTAATGAGCATATCTTTTATCGTTGACGACATCTTCGATCTATCTAA 7 0.17500000000000002 No Hit AGGTGACTGTGCGGAAGCTGAATCTGAATTTCTGCTGGTCTGAGGAGGAC 7 0.17500000000000002 No Hit GAAACTGAAGAATGACGCTTAATAGGAAAACGCACAACATCTTGTTTATT 7 0.17500000000000002 No Hit CTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCT 6 0.15 No Hit CACAGTACATAACCAGATTCTGATAGCTTATAATTTGGAACGAATTCATG 6 0.15 No Hit GCCACGTTCTCCCTTTGGAAGTTAAAGAACACAAACCTTCCCAAAAAGAG 6 0.15 No Hit GCAACCTTGCATTGCAAGCTTGGCAAGTTTTGACAAATAGTTAATGCATG 6 0.15 No Hit CGGCAGGATGAATAATATCGAGCAATTTCATAATAGTAGCGCCGTCATTA 6 0.15 No Hit ATAAAAATGAAGAGAGCTGTATTTTTACATGGGAAATTTTGCATAAAAGA 6 0.15 No Hit TCTCTGTGAAAGTCTTCCATAGCCTAACATTAAACCTTCTCTTACACTCA 6 0.15 No Hit GGAACAATCACATCTTTCAGCAGTGAGACTTGGGTATGCTGTATCATATC 6 0.15 No Hit AGCCCGATCACGAAGAGCCTTGTAAGCCTCAATGACCAAAATCACCTCGT 6 0.15 No Hit CCACCCTGCAATGGTCCTGATTTATGCATCCAGGGGACCATAAACACCTG 6 0.15 No Hit CTTTTAGCAGCGAAAACGGTGAGAGACTGGAACCTTTGAGTGAGTGAGGT 6 0.15 No Hit TGCTGTTCCTAGTTAATAGGAAGAAGGTAAGCTAGCATTAATATCCTTGT 5 0.125 No Hit GTCAGTTACCTCACCGAGTGGCCCGCCAGCAATTCTGTAACCCTCAACGG 5 0.125 No Hit TGGTAGTCTTATACAAGGAAGATTCAACTCTTTACAGATTATCTCTTGAA 5 0.125 No Hit TTCCACGCACTACTGCACAAATTCGTATACATGATAGCTTAAATTTTGGT 5 0.125 No Hit TCCTCTATCCTCTTCTTCTTCCTCTTCATACTCCTCTTCTTCATCCATCA 5 0.125 No Hit CAGGTAACAGACCCCTTTAGCATATATGGTACAAGCAAGCTTGCCAAGGG 5 0.125 No Hit CAGTGATCAAGATACAATCCAGTACACAATATCTGAAAATTCTCTCCGCC 5 0.125 No Hit AGCAGAATGGTTTACACGAGAGGGAATAGTAGAAAGGAGATTCAGCTGGC 5 0.125 No Hit TCCGTAATCACCAACCAAGCTGCCATCTAGGTACTCAGGTGCCTTGGCTC 5 0.125 No Hit CCAGTCATCAAAATGGATCAGCAGCCAACAGAAGCTTTCAGTTAAATTTA 5 0.125 No Hit CCTCAGCTGCTCAACAGCTCCTGACCTAAATGTATCACAAGCAGCCATCA 5 0.125 No Hit CTCCACTCTTCTTCTTCTGGGGGACAGCAACAGAGTTCTTGCGTCCACTG 5 0.125 No Hit GGACAGAGGCATTCCAGGTCCCACTGTCTTCTCAACAATCTTCAGGGGAA 5 0.125 No Hit AGTTAGTGAAAGTGCATAAGACAACGAAAGCCCCACGAGTCCTGGAGGTA 5 0.125 No Hit GTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGA 5 0.125 No Hit GCTGAAAGGAGAGAAAAAGAGAAGCGGGCTTGATAGAAGAAGTGGAGGAG 5 0.125 No Hit GGCTAGATGCAGGACTTGTCCTACTATTGTTCTCTTTTGTAGTAGAGTCG 5 0.125 No Hit CTTCAATATTTTGTTTAATCTGCTCAAGTGTCCAGACAAGACATGGCTGA 5 0.125 No Hit CAACATTTGAAGTCATTATCAGGAGGGTGTTTTTGAAGTCTACAGTCCTG 5 0.125 No Hit CGAGGGATTGCTGACAGGTTACAGCTAACACTGATATAAACAGGCTTGCT 5 0.125 No Hit GCCCATTCATTACTCCATTAGCAACGGATTTGGCCTTCAACTGTTCCTTT 5 0.125 No Hit GCCCATTCCAAAAGAAAGTTACTGCAAGCCCTTTGGTTTCCACATTCCTA 5 0.125 No Hit GCTTGGCAAGATCTTGGCAGCCTGTGAAGTTCTCGGGGAACTTGCATGTG 5 0.125 No Hit CGAGAATATTGCAAACCTTAACTTCATCTCCCCCTCTGTCTCATACCTCT 5 0.125 No Hit CCCCTTCCGCTGTAGCTATCTTCTGCTGACTCTCCTCCTGCAAACTCTCC 5 0.125 No Hit GAGCTTAGTAGGCGTAGTTCTTGACGAACATTCCTTTCTTGGCTTCATCT 5 0.125 No Hit GCACGTCTGAGATCACGGTGGCTAGCATCAAGCAGTCTCTGATCTCCAGT 5 0.125 No Hit CTCAAGTCACGGTGTGAAGCATCCAAGAGCCTCTGATCTCCAGTGTAGGA 5 0.125 No Hit TCTCTATATTTCTTACTGAAGCTAAAGGTGAAATTCCTATAGTCTGATAT 5 0.125 No Hit CCCCAATCTTGATTTCATAAGCCTCCGGTTCATTCAAAATGAACTCCTTC 5 0.125 No Hit CTCCAGCTATCTATAGAAGAACGTGCCCGCACAAGCTTCTCTGAAGTGTT 5 0.125 No Hit GCAGAATGTTAGCAAGCTGAGAATTACTGTTTATGGCAGCTTTAACCTTC 5 0.125 No Hit GCCACTGAGAAAGTTTGTGGAGAGATCGAGAATTTGGAGGGACGACAAAT 5 0.125 No Hit CCCCGGCTGAGTTAACGATGGGCCAGAAGGTGTCCTTGGTGACCTCAGTG 5 0.125 No Hit GGGAAGTGAGATTGTTGAGGTGGATGGTGTGTTCTCCCACCTTCACCATC 5 0.125 No Hit CATGAGCTCGTTACATCACAAGTTAACATTACAAGCCAATAGTCCCAGCA 5 0.125 No Hit CACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCC 5 0.125 No Hit GTCGCATTTTATGTTCTTGTAGATCCTCCTCACAAACAAATTTGAGCCTA 5 0.125 No Hit GGGCTCCTTCTAACGCCCCGGTAGCACTAAACGACCAGCACGATCCACAC 5 0.125 No Hit TCCAATCCATCGATCCCCTGTAACTGGTTTCTTAACCTCATCTTTTTCAG 5 0.125 No Hit CTCCATTACCAAACACAGGCCTTGATCTTTTCTTCGTGGAAGCAACCTGT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0125 0.0 0.0 0.0 0.0 82-83 0.1125 0.0 0.0 0.0 0.0 84-85 0.1875 0.0 0.0 0.0 0.0 86-87 0.25 0.0 0.0 0.0 0.0 88-89 0.3125 0.0 0.0 0.0 0.0 90-91 0.45 0.0 0.0 0.0 0.0 92-93 0.65 0.0 0.0 0.0 0.0 94-95 0.8125 0.0 0.0 0.0 0.0 96-97 0.8625 0.0 0.0 0.0 0.0 98-99 0.9125000000000001 0.0 0.0 0.0 0.0 100-101 0.95 0.0 0.0 0.0 0.0 102-103 1.0125 0.0 0.0 0.0 0.0 104-105 1.1375000000000002 0.0 0.0 0.0 0.0 106-107 1.35 0.0 0.0 0.0 0.0 108-109 1.425 0.0 0.0 0.0 0.0 110-111 1.55 0.0 0.0 0.0 0.0 112-113 1.8375 0.0 0.0 0.0 0.0 114-115 1.975 0.0 0.0 0.0 0.0 116-117 2.2625 0.0 0.0 0.0 0.0 118-119 2.4625 0.0 0.0 0.0 0.0 120-121 2.7249999999999996 0.0 0.0 0.0 0.0 122-123 2.9 0.0 0.0 0.0 0.0 124-125 3.25 0.0 0.0 0.0 0.0 126-127 3.6625 0.0 0.0 0.0 0.0 128-129 4.0 0.0 0.0 0.0 0.0 130-131 4.2375 0.0 0.0 0.0 0.0 132-133 4.6625 0.0 0.0 0.0 0.0 134-135 5.25 0.0 0.0 0.0 0.0 136-137 5.6 0.0 0.0 0.0 0.0 138-139 5.9875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCCTAT 10 0.006830828 145.0 1 >>END_MODULE SRR13695447 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695447_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.04975 37.0 37.0 37.0 37.0 37.0 2 36.1045 37.0 37.0 37.0 37.0 37.0 3 35.9755 37.0 37.0 37.0 37.0 37.0 4 36.098 37.0 37.0 37.0 37.0 37.0 5 36.1615 37.0 37.0 37.0 37.0 37.0 6 36.1205 37.0 37.0 37.0 37.0 37.0 7 36.1735 37.0 37.0 37.0 37.0 37.0 8 36.1765 37.0 37.0 37.0 37.0 37.0 9 36.188 37.0 37.0 37.0 37.0 37.0 10-14 36.1581 37.0 37.0 37.0 37.0 37.0 15-19 36.137699999999995 37.0 37.0 37.0 37.0 37.0 20-24 36.14665 37.0 37.0 37.0 37.0 37.0 25-29 36.073449999999994 37.0 37.0 37.0 37.0 37.0 30-34 36.05215 37.0 37.0 37.0 37.0 37.0 35-39 36.02905 37.0 37.0 37.0 37.0 37.0 40-44 35.988749999999996 37.0 37.0 37.0 37.0 37.0 45-49 36.060449999999996 37.0 37.0 37.0 37.0 37.0 50-54 35.976150000000004 37.0 37.0 37.0 37.0 37.0 55-59 35.954950000000004 37.0 37.0 37.0 37.0 37.0 60-64 35.96365 37.0 37.0 37.0 37.0 37.0 65-69 35.88815 37.0 37.0 37.0 37.0 37.0 70-74 35.86445 37.0 37.0 37.0 37.0 37.0 75-79 35.87015 37.0 37.0 37.0 37.0 37.0 80-84 35.837149999999994 37.0 37.0 37.0 37.0 37.0 85-89 35.718050000000005 37.0 37.0 37.0 37.0 37.0 90-94 35.72965 37.0 37.0 37.0 37.0 37.0 95-99 35.766349999999996 37.0 37.0 37.0 37.0 37.0 100-104 35.73615 37.0 37.0 37.0 37.0 37.0 105-109 35.62245 37.0 37.0 37.0 37.0 37.0 110-114 35.615950000000005 37.0 37.0 37.0 37.0 37.0 115-119 35.64835 37.0 37.0 37.0 37.0 37.0 120-124 35.58335000000001 37.0 37.0 37.0 37.0 37.0 125-129 35.60815 37.0 37.0 37.0 37.0 37.0 130-134 35.44715 37.0 37.0 37.0 37.0 37.0 135-139 35.4497 37.0 37.0 37.0 37.0 37.0 140-144 35.370349999999995 37.0 37.0 37.0 34.6 37.0 145-149 35.302350000000004 37.0 37.0 37.0 32.2 37.0 150-151 35.09925 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 1.0 20 2.0 21 1.0 22 1.0 23 8.0 24 3.0 25 15.0 26 11.0 27 22.0 28 15.0 29 23.0 30 29.0 31 45.0 32 69.0 33 98.0 34 238.0 35 590.0 36 2660.0 37 167.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.208072198546 15.342191025319629 21.133116069190272 33.31662070694409 2 27.775 22.85 29.725 19.650000000000002 3 22.15 24.425 30.599999999999998 22.825 4 25.724999999999998 31.624999999999996 23.325000000000003 19.325 5 24.675 35.725 22.1 17.5 6 18.9 38.224999999999994 25.1 17.775 7 20.1 20.424999999999997 40.949999999999996 18.525 8 22.825 25.5 28.050000000000004 23.625 9 22.325 24.75 31.7 21.224999999999998 10-14 22.78 29.215000000000003 26.985 21.02 15-19 22.46 28.139999999999997 28.595 20.805 20-24 21.99549887471868 27.89197299324831 29.15728932233058 20.955238809702426 25-29 22.23555888972243 28.927231807951987 27.886971742935735 20.950237559389848 30-34 22.550637659414853 28.232058014503625 28.49212303075769 20.72518129532383 35-39 22.78569642410603 28.557139284821204 27.646911727931982 21.010252563140785 40-44 22.290572643160793 28.712178044511127 27.826956739184794 21.170292573143286 45-49 22.02050512628157 28.877219304826205 27.431857964491122 21.670417604401102 50-54 23.060765191297826 28.012003000750184 27.726931732933235 21.200300075018756 55-59 22.740685171292824 28.832208052013 27.996999249812454 20.43010752688172 60-64 22.13053263315829 28.632158039509875 28.577144286071515 20.660165041260314 65-69 22.953443016452468 27.774166124918736 27.71915787368105 21.553232984947744 70-74 22.59064766191548 28.212053013253314 27.326831707926978 21.870467616904225 75-79 22.95573893473368 27.551887971993 28.24206051512878 21.250312578144538 80-84 22.885721430357588 28.017004251062765 27.53188297074269 21.56539134783696 85-89 23.455863965991497 28.52213053263316 27.631907976994246 20.390097524381094 90-94 23.045761440360092 29.022255563890976 27.28182045511378 20.650162540635158 95-99 23.455863965991497 28.217054263565895 27.561890472618156 20.765191297824455 100-104 23.655913978494624 28.24206051512878 27.346836709177296 20.7551887971993 105-109 23.615903975993998 27.106776694173547 28.28207051762941 20.99524881220305 110-114 23.43085771442861 27.53188297074269 28.132033008252062 20.905226306576644 115-119 23.670917729432357 28.257064266066518 27.95198799699925 20.120030007501878 120-124 23.96599149787447 28.08202050512628 28.22705676419105 19.724931232808203 125-129 23.66591647911978 28.322080520130033 27.58689672418104 20.425106276569142 130-134 24.7136497774221 27.604661631571048 27.354574100935324 20.327114490071523 135-139 25.052515754726418 28.26848054416325 26.958087426227866 19.720916274882462 140-144 25.81645411352838 28.41210302575644 26.646661665416353 19.124781195298823 145-149 25.646411602900727 26.916729182295573 27.431857964491122 20.005001250312578 150-151 27.426213106553277 27.33866933466733 25.887943971985994 19.3471735867934 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 2.0 7 2.0 8 0.5 9 1.0 10 0.5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 2.0 17 1.5 18 0.0 19 0.0 20 0.5 21 2.0 22 5.0 23 4.0 24 2.5 25 4.5 26 8.0 27 8.5 28 8.0 29 15.5 30 20.0 31 25.5 32 36.0 33 46.0 34 49.5 35 60.5 36 80.0 37 112.0 38 148.0 39 181.5 40 226.0 41 246.5 42 268.0 43 277.5 44 243.0 45 230.0 46 253.0 47 243.5 48 195.5 49 177.0 50 157.0 51 134.0 52 116.5 53 91.0 54 80.5 55 57.0 56 38.5 57 34.5 58 27.0 59 18.5 60 13.0 61 13.5 62 11.0 63 3.5 64 1.0 65 1.0 66 1.5 67 1.5 68 0.0 69 0.0 70 0.0 71 0.0 72 0.5 73 1.0 74 1.0 75 0.5 76 0.5 77 0.5 78 0.0 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27499999999999997 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.025 25-29 0.025 30-34 0.025 35-39 0.025 40-44 0.025 45-49 0.025 50-54 0.025 55-59 0.025 60-64 0.025 65-69 0.015 70-74 0.025 75-79 0.025 80-84 0.025 85-89 0.025 90-94 0.025 95-99 0.025 100-104 0.025 105-109 0.025 110-114 0.025 115-119 0.025 120-124 0.025 125-129 0.025 130-134 0.034999999999999996 135-139 0.03 140-144 0.025 145-149 0.025 150-151 0.05 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.25 #Duplication Level Percentage of deduplicated Percentage of total 1 68.39080459770115 44.625 2 18.88888888888889 24.65 3 7.432950191570881 14.549999999999999 4 3.103448275862069 8.1 5 1.3793103448275863 4.5 6 0.45977011494252873 1.7999999999999998 7 0.19157088122605362 0.8750000000000001 8 0.11494252873563218 0.6 9 0.0 0.0 >10 0.03831417624521073 0.3 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTCAATTGGCTGTTTGTCAAGGGATTGCTTGGCTGGTTTATGCAGGTTGC 12 0.3 No Hit CCTCCCTCTCAATCCCTTTGTTTTCCTCCAATGATAAATGAACTTGGAGG 8 0.2 No Hit GTGCAACTTTTCAACAGGTTTACATTGCTATCGAGGGGCTAGAAAAAAGC 8 0.2 No Hit GTGGAAGATGGCTGGATTTTTGTCTGAAAACCGTCCAGTGCAGACTCTTA 8 0.2 No Hit AAGGAGTCTGCAAAATTGGCTGATAGGTTTCGTTCTGGTCTCTCCACCTA 7 0.17500000000000002 No Hit GCTTAACTGGGTTCTGGGAGGAGGGCTCTTGGCTATATACATGATGTCTG 7 0.17500000000000002 No Hit GTCCTTTTTATCTTTGGGATAGTTGAAAAATGTACAAGACATGAGAAAAT 7 0.17500000000000002 No Hit TTTGTTTCGAAGAAGCTAGCTACCTTTTATCTAATTAGCTCCGCAATTCC 7 0.17500000000000002 No Hit CTCAAACAAACAACAATCACCTTCAATACATTCATTTCTCTCTCTCTCTC 7 0.17500000000000002 No Hit CTCGGCGGATGCTCTGGTTAAGCGATTCGTTGACACCAACTCATCCGCCG 6 0.15 No Hit TCGGATGCGTGGAAGCGATCTGGCGGGAAAGATCATGTCTTTGTTCTTAC 6 0.15 No Hit GTCCATTCATTGCTGGAGAAAAGATCACTGCTGTAGATTTGAGCTTGGCT 6 0.15 No Hit CACGACGCGCTTTCCTCCTTCTTATTCTTCCTTCAAAACGTTAGCAGAAA 6 0.15 No Hit CAGGATCTGTTAACTCATCTTTTAAGGAGCCTTGCAAGCCATAGTGTTGA 6 0.15 No Hit TCATAATCCAAGCCTCTCTTCAACTCACAAGGCAGGCGCACTTCCACCGA 6 0.15 No Hit CATGAATATGGGACTACAAGTTCCTGCATTCAGAATGCCTTACTCAACTC 6 0.15 No Hit CAGCCATGATGCAACCGCAGATCATACTACTGAAAGAAGGAACCGACACG 6 0.15 No Hit GAGATTTTCTTGCAACAGCCTAATTTGCTGGAGCTTGAAGCATCCATCAA 6 0.15 No Hit GCAACTAAAAGAAGATATCGATGCTATTATGACAGCCCCGCGACCTCTGC 6 0.15 No Hit CGCGTTTCATGGGCTTTCATATGCAAGCCGCTTCTCAGCCCATGAATATG 6 0.15 No Hit CTCAATGTTCGGATTCTTTGTCCAGGCCATTGTGACCGGAAAGGGACCAC 6 0.15 No Hit CTTGAAACTGACACTAAGAACACTACAGAAGAAAGTGATGAAAAGTCGAA 5 0.125 No Hit CACAGTGCAGGCTGCTATGGAAGAAGCACTTGTCCGTATTTTAACTCCTA 5 0.125 No Hit TGAAGGACTCGTGTGCTAAACACGGCGGCGACGCCTTAATCCACCGTGTT 5 0.125 No Hit TGAAATGACTGTATTGTTTTCTCGAGAAAATCTGGAGTTTAATTGATTTA 5 0.125 No Hit TCTATATCCACCACTTGGTTTCTCCTTGCAAAACACACAAGAAAAGAAGG 5 0.125 No Hit CATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAA 5 0.125 No Hit TGCTGGAAGGAATTTCTGAGATTGAGAGACCACCGGCTCCAAAGACAATA 5 0.125 No Hit ATGAAAATGCCTTCCATATCTGAACTTGCTGCTGCTTTAGAGAACAAACA 5 0.125 No Hit CACATATAGAGGGTGTAATAGCTAAGTAGCCTGTAAGAGATGGCTTCCTC 5 0.125 No Hit CCTTGCAGTACCCATGGTCCAAACCCGAAGATCCTTCAAAAATCAAGAAC 5 0.125 No Hit CCCTGGTGCTGGGAAACATATTCAAGCTGGTGCCACGAAAGTTATCATCA 5 0.125 No Hit GCGAGGGAGAAGTTGGCTGAATATGTTGAAGAGCGGCAGGTACTCTTAGC 5 0.125 No Hit TTTGGATACACTTTGATGTTCTGTCTTGGATTGGGAATTCTCTGTGGAGC 5 0.125 No Hit CTCAGTTTTGTAATAACACATTTTGCAAGCAATGGCAGCCATGAATTCTA 5 0.125 No Hit AGTACACCTTGAGATACACAGGAGGGATGGTGCCAGATGTCAATCAGATC 5 0.125 No Hit GGCTCGTGTCGCTGGGTTGGTGGTCTCATCGATCTTGTTCCTGCTATGCT 5 0.125 No Hit GGACTCAGTGTGCTGAATGCGATTGCTGGTGCTTACAGTGAGAATTTGCC 5 0.125 No Hit AGTTGACGGAGGCTGTTAGGCGTCGCCCTTACACTGTGGTACTCTTTGAT 5 0.125 No Hit CATTCGGAAATCCTTTCAGGATGCTATCTTCAAAGGGTTCTCAACTGTCC 5 0.125 No Hit AGGTCACTGAAGGCACCGCGAAATGGACTGTTGAATTTATAAAGAAACAT 5 0.125 No Hit TATGAAATGGACAAAGATTATGAAAACGGGAGGAATTCGAGTGCAGTTTT 5 0.125 No Hit AATCTAACCACGAACAAGTGTGGTAGGTACACTAATGAAACATAAATTTA 5 0.125 No Hit GGAGCTGGAAGAACAGCTGTTCCACAGCAGCCACGGCAACCTTCACCTCA 5 0.125 No Hit CAACAATGGCTTCGATCCAGTTCACACTGTCTCCTACTTCCTCCATCCGC 5 0.125 No Hit GCTCGAGTACTGAGCCAACCATCTTTGTCTGGGTTGCAGGGGTTGGTCCT 5 0.125 No Hit GTTAGGGTTAGCGCCCATACTCTCTCTATCTCGCTCTCCGATCGCAATTG 5 0.125 No Hit CTCCCATTCCGCAACTCTCCAAAAAACATACGCAGTTAACGTTAGACTCC 5 0.125 No Hit AACGCCTGATATCATAAACACTCCGTCCACCAAACCGAACAGCACTAAAT 5 0.125 No Hit TTTAGCTTCTGCAAGGGAACTAATAAGAATTAATGGAACAACAAAGGCTC 5 0.125 No Hit AGAAACAATAGCTTCAGACTTGGAATCTATTGGAAAAGATATTGCAAAGA 5 0.125 No Hit CCTGATTCTCTGACTCCTGATCAGGTTAAAGCAATAGAGTCTATTCTGCC 5 0.125 No Hit AACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAA 5 0.125 No Hit CATAGTGCGACGGCTGATGGTGTTATCCAACAAAGTTCTGAAAACAATTC 5 0.125 No Hit CAAACAAGGGGTGCTAGAAGATAAGAAGATGGCTTCTCTTCCCATCGCCT 5 0.125 No Hit TGGCATTTGAACTGTATGGAATGTTGGCTGGGAGTGTCAGTCCAATGACG 5 0.125 No Hit TCTGGGTGTTAGATGGAGGTCTTCCAAGATGGCGTGCTTCAGGGTATGAT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.1375 0.0 0.0 0.0 0.0 84-85 0.21250000000000002 0.0 0.0 0.0 0.0 86-87 0.275 0.0 0.0 0.0 0.0 88-89 0.3375 0.0 0.0 0.0 0.0 90-91 0.475 0.0 0.0 0.0 0.0 92-93 0.675 0.0 0.0 0.0 0.0 94-95 0.8375 0.0 0.0 0.0 0.0 96-97 0.8875 0.0 0.0 0.0 0.0 98-99 0.9375 0.0 0.0 0.0 0.0 100-101 0.975 0.0 0.0 0.0 0.0 102-103 1.0375 0.0 0.0 0.0 0.0 104-105 1.1625 0.0 0.0 0.0 0.0 106-107 1.325 0.0 0.0 0.0 0.0 108-109 1.4 0.0 0.0 0.0 0.0 110-111 1.525 0.0 0.0 0.0 0.0 112-113 1.8125 0.0 0.0 0.0 0.0 114-115 1.9375 0.0 0.0 0.0 0.0 116-117 2.2125000000000004 0.0 0.0 0.0 0.0 118-119 2.5125 0.0 0.0 0.0 0.0 120-121 2.7750000000000004 0.0 0.0 0.0 0.0 122-123 2.975 0.0 0.0 0.0 0.0 124-125 3.325 0.0 0.0 0.0 0.0 126-127 3.75 0.0 0.0 0.0 0.0 128-129 4.1 0.0 0.0 0.0 0.0 130-131 4.3375 0.0 0.0 0.0 0.0 132-133 4.762499999999999 0.0 0.0 0.0 0.0 134-135 5.375 0.0 0.0 0.0 0.0 136-137 5.762499999999999 0.0 0.0 0.0 0.0 138-139 6.2125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGAATCC 10 0.006830828 145.0 3 >>END_MODULE Read 1110277 spots for SRR13695447.sra Written 1110277 spots for SRR13695447.sra Read 1110277 spots for SRR13695447.sra Written 1110277 spots for SRR13695447.sra Read 1110277 spots for SRR13695447.sra Written 1110277 spots for SRR13695447.sra Read 1110277 spots for SRR13695447.sra Written 1110277 spots for SRR13695447.sra Read 1110277 spots for SRR13695447.sra Written 1110277 spots for SRR13695447.sra Read 1110277 spots for SRR13695447.sra Written 1110277 spots for SRR13695447.sra Read 1110277 spots for SRR13695447.sra Written 1110277 spots for SRR13695447.sra Read 1110277 spots for SRR13695447.sra Written 1110277 spots for SRR13695447.sra Read 1110277 spots for SRR13695447.sra Written 1110277 spots for SRR13695447.sra Read 1110277 spots for SRR13695447.sra Written 1110277 spots for SRR13695447.sra Read 1110277 spots for SRR13695447.sra Written 1110277 spots for SRR13695447.sra Read 1110277 spots for SRR13695447.sra Written 1110277 spots for SRR13695447.sra Read 1110283 spots for SRR13695447.sra Written 1110283 spots for SRR13695447.sra Read 1110277 spots for SRR13695447.sra Written 1110277 spots for SRR13695447.sra Read 1110277 spots for SRR13695447.sra Written 1110277 spots for SRR13695447.sra Read 1110277 spots for SRR13695447.sra Written 1110277 spots for SRR13695447.sra Read 1110277 spots for SRR13695447.sra Written 1110277 spots for SRR13695447.sra Read 1110277 spots for SRR13695447.sra Written 1110277 spots for SRR13695447.sra Read 1110277 spots for SRR13695447.sra Written 1110277 spots for SRR13695447.sra Read 1110277 spots for SRR13695447.sra Written 1110277 spots for SRR13695447.sra SRR ids: ['SRR13695447.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_grdmgjqg SRR13695447.sra spots: 22205546 blocks: [[1, 1110277], [1110278, 2220554], [2220555, 3330831], [3330832, 4441108], [4441109, 5551385], [5551386, 6661662], [6661663, 7771939], [7771940, 8882216], [8882217, 9992493], [9992494, 11102770], [11102771, 12213047], [12213048, 13323324], [13323325, 14433601], [14433602, 15543878], [15543879, 16654155], [16654156, 17764432], [17764433, 18874709], [18874710, 19984986], [19984987, 21095263], [21095264, 22205546]] SRR13695447 file size 7524715 SRR13695447 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695447 SRR13695447_1.fastq SRR13695447_2.fastq Input file: SRR13695447_1.fastq Paired file: SRR13695447_2.fastq trimmed: SRR13695447-trimmed-pair1.fastq, SRR13695447-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 03:38:48 2025 >> started Wed Feb 12 03:39:15 2025 >> done (26.857s) 22205546 read pairs processed; of these: 154 ( 0.00%) short read pairs filtered out after trimming by size control 38932 ( 0.18%) empty read pairs filtered out after trimming by size control 22166460 (99.82%) read pairs available; of these: 2160371 ( 9.75%) trimmed read pairs available after processing 20006089 (90.25%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 3 0.00% 20 5 0.00% 21 1 0.00% 22 4 0.00% 23 2 0.00% 24 1 0.00% 25 4 0.00% 26 8 0.00% 27 1 0.00% 28 0 0.00% 29 7 0.00% 30 7 0.00% 31 9 0.00% 32 7 0.00% 33 7 0.00% 34 11 0.00% 35 10 0.00% 36 14 0.00% 37 14 0.00% 38 19 0.00% 39 25 0.00% 40 29 0.00% 41 33 0.00% 42 34 0.00% 43 32 0.00% 44 32 0.00% 45 55 0.00% 46 43 0.00% 47 89 0.00% 48 81 0.00% 49 83 0.00% 50 115 0.00% 51 114 0.00% 52 169 0.00% 53 171 0.00% 54 171 0.00% 55 147 0.00% 56 244 0.00% 57 260 0.00% 58 270 0.00% 59 343 0.00% 60 389 0.00% 61 445 0.00% 62 489 0.00% 63 547 0.00% 64 712 0.00% 65 776 0.00% 66 688 0.00% 67 843 0.00% 68 916 0.00% 69 1123 0.01% 70 1295 0.01% 71 1410 0.01% 72 1679 0.01% 73 1889 0.01% 74 2287 0.01% 75 2239 0.01% 76 2658 0.01% 77 2828 0.01% 78 2970 0.01% 79 3473 0.02% 80 3657 0.02% 81 4246 0.02% 82 4588 0.02% 83 5196 0.02% 84 5805 0.03% 85 6460 0.03% 86 6799 0.03% 87 7017 0.03% 88 7685 0.03% 89 7904 0.04% 90 8471 0.04% 91 8911 0.04% 92 9720 0.04% 93 10493 0.05% 94 11259 0.05% 95 12247 0.06% 96 12864 0.06% 97 13448 0.06% 98 13571 0.06% 99 14129 0.06% 100 15012 0.07% 101 15695 0.07% 102 16773 0.08% 103 17650 0.08% 104 18382 0.08% 105 19336 0.09% 106 20396 0.09% 107 20975 0.09% 108 21682 0.10% 109 22706 0.10% 110 22799 0.10% 111 23779 0.11% 112 24471 0.11% 113 25266 0.11% 114 26351 0.12% 115 27976 0.13% 116 28825 0.13% 117 30288 0.14% 118 30894 0.14% 119 31293 0.14% 120 32539 0.15% 121 32949 0.15% 122 34079 0.15% 123 35236 0.16% 124 36468 0.16% 125 37703 0.17% 126 39308 0.18% 127 40302 0.18% 128 41108 0.19% 129 41433 0.19% 130 42725 0.19% 131 43716 0.20% 132 44018 0.20% 133 45858 0.21% 134 46215 0.21% 135 47974 0.22% 136 49268 0.22% 137 50804 0.23% 138 51221 0.23% 139 52552 0.24% 140 53704 0.24% 141 54415 0.25% 142 55731 0.25% 143 56520 0.25% 144 57483 0.26% 145 59067 0.27% 146 59539 0.27% 147 60581 0.27% 148 62665 0.28% 149 62973 0.28% 150 64866 0.29% 151 20006089 90.25% 22166460 reads passed initial QC criterion=sequence-density sequence-density=0.38 sequence-density-rank=1 fanout-score=2.19 fanout-score-rank=34 prefix-density=0.40 prefix-fanout=2.1 sequence=TGAGATGCCTCAGTGCATCCAAACATGGGTAGCTTCCACATAGTCCAGTAGCGTCCATCATAGTACCCTGGTGAGCTGTGGTGCTCACGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTTCGAGCGAAGAAGGTAATCAATTTCCTTGGCCAATTCCTCCTCTGTGAGATCTGGAAGGTAAGAAAGAGTCTCGAACTTCTTCAATCCAGTTGGTGGCCACACCTGCATGCATTGAACTCGTCCACCATTGCTTGCAATGGAAGTAATGTCATTAGCCTTTCTGGTACTGACTGGGAAAGCTGCGGCAGACTTGAGACCATTGAATGGTGCCACCATGTTGGCTTGTACCGGGGTGCGGTTGACGGTGGCAACGGCTGCCGATGAGATCATAGAGGAGGAAGCCAT criterion=fanout-score sequence-density=0.01 sequence-density-rank=39 fanout-score=515.77 fanout-score-rank=1 prefix-density=0.25 prefix-fanout=17.5 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGG criterion=sequence-density sequence-density=0.56 sequence-density-rank=1 fanout-score=2.09 fanout-score-rank=33 prefix-density=0.55 prefix-fanout=2.1 sequence=CCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAATCATCATGTCTAGCACCTGCGACACCTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAGTGAACAAACAAAAGTGTCCGTGAGTGTCTTTATCTTTGTGTCTTCTTCA criterion=fanout-score sequence-density=0.12 sequence-density-rank=21 fanout-score=36.73 fanout-score-rank=1 prefix-density=0.35 prefix-fanout=12.6 sequence=AAAGAAAAGAAAA SRR13695447 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 03:39:55 Started mapping on | Feb 12 03:39:55 Finished on | Feb 12 03:42:06 Mapping speed, Million of reads per hour | 609.15 Number of input reads | 22166460 Average input read length | 297 UNIQUE READS: Uniquely mapped reads number | 20810895 Uniquely mapped reads % | 93.88% Average mapped length | 295.75 Number of splices: Total | 20857263 Number of splices: Annotated (sjdb) | 20402849 Number of splices: GT/AG | 20431588 Number of splices: GC/AG | 341876 Number of splices: AT/AC | 11851 Number of splices: Non-canonical | 71948 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.03% Deletion average length | 2.87 Insertion rate per base | 0.02% Insertion average length | 2.36 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 487254 % of reads mapped to multiple loci | 2.20% Number of reads mapped to too many loci | 129703 % of reads mapped to too many loci | 0.59% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.08% % of reads unmapped: other | 0.26% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 868620 868620 868620 N_multimapping 487254 487254 487254 N_noFeature 879929 20311117 1185968 N_ambiguous 314237 2089 118897 UnstrandedReadsAssigned:19616729 PositiveStrandReadsAssigned:497689 NegativeStrandReadsAssigned:19506030 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695447 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695447-trimmed-pair1.fastq SRR13695447-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 22,166,460 reads, 19,564,450 reads pseudoaligned [quant] estimated average fragment length: 252.729 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,111 rounds 52401 SRR13695447.ke.tsv 34699 SRR13695447.se.tsv 87100 total ==> SRR13695447.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1766.27 619 16.76 Potri.005G024800.1.v4.1 1035 783.271 183 11.1733 Potri.004G059700.1.v4.1 961 709.346 11 0.741609 Potri.007G009000.2.v4.1 1416 1164.27 0 0 Potri.003G141000.2.v4.1 2943 2691.27 1258 22.3544 Potri.016G087400.1.v4.1 270 81.5133 943 553.253 Potri.015G069301.1.v4.1 564 321.488 0 0 Potri.010G195200.1.v4.1 1773 1521.27 59 1.85475 Potri.012G127500.1.v4.1 977 725.312 108 7.12098 ==> SRR13695447.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 159 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 310 Potri.001G212900.v4.1 11 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 7 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR13695447 completed mapping pipeline successfully