Starting /dee2/code/volunteer_pipeline.sh SRR13695448 current disk space = 3048958296064 free memory = 1579249560 SRR13695448 SRAfilesize dd9771e24cb6d20b54451575c829424c SRR13695448.sra SRR13695448.sra file validated SRR13695448 is paired end SRR13695448 is conventional basespace SRR13695448 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695448_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.4705 37.0 37.0 37.0 37.0 37.0 2 36.2625 37.0 37.0 37.0 37.0 37.0 3 36.487 37.0 37.0 37.0 37.0 37.0 4 36.5505 37.0 37.0 37.0 37.0 37.0 5 36.708 37.0 37.0 37.0 37.0 37.0 6 36.6045 37.0 37.0 37.0 37.0 37.0 7 36.452 37.0 37.0 37.0 37.0 37.0 8 36.5745 37.0 37.0 37.0 37.0 37.0 9 36.5555 37.0 37.0 37.0 37.0 37.0 10-14 36.5699 37.0 37.0 37.0 37.0 37.0 15-19 36.4795 37.0 37.0 37.0 37.0 37.0 20-24 36.5056 37.0 37.0 37.0 37.0 37.0 25-29 36.4277 37.0 37.0 37.0 37.0 37.0 30-34 36.435 37.0 37.0 37.0 37.0 37.0 35-39 36.3717 37.0 37.0 37.0 37.0 37.0 40-44 36.39960000000001 37.0 37.0 37.0 37.0 37.0 45-49 36.3654 37.0 37.0 37.0 37.0 37.0 50-54 36.3365 37.0 37.0 37.0 37.0 37.0 55-59 36.3116 37.0 37.0 37.0 37.0 37.0 60-64 36.3176 37.0 37.0 37.0 37.0 37.0 65-69 36.2869 37.0 37.0 37.0 37.0 37.0 70-74 36.307900000000004 37.0 37.0 37.0 37.0 37.0 75-79 36.2157 37.0 37.0 37.0 37.0 37.0 80-84 36.1695 37.0 37.0 37.0 37.0 37.0 85-89 36.1871 37.0 37.0 37.0 37.0 37.0 90-94 36.1529 37.0 37.0 37.0 37.0 37.0 95-99 36.04709999999999 37.0 37.0 37.0 37.0 37.0 100-104 36.0439 37.0 37.0 37.0 37.0 37.0 105-109 36.0757 37.0 37.0 37.0 37.0 37.0 110-114 36.03680000000001 37.0 37.0 37.0 37.0 37.0 115-119 35.999399999999994 37.0 37.0 37.0 37.0 37.0 120-124 35.95700000000001 37.0 37.0 37.0 37.0 37.0 125-129 35.8871 37.0 37.0 37.0 37.0 37.0 130-134 35.8363 37.0 37.0 37.0 37.0 37.0 135-139 35.7432 37.0 37.0 37.0 37.0 37.0 140-144 35.6672 37.0 37.0 37.0 37.0 37.0 145-149 35.3837 37.0 37.0 37.0 37.0 37.0 150-151 35.199 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 1.0 23 0.0 24 2.0 25 5.0 26 6.0 27 5.0 28 17.0 29 19.0 30 24.0 31 38.0 32 58.0 33 99.0 34 131.0 35 360.0 36 2934.0 37 301.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.975 11.200000000000001 9.275 46.550000000000004 2 18.925702811244978 15.010040160642571 39.708835341365464 26.35542168674699 3 18.925 18.325 26.55 36.199999999999996 4 21.95 25.124999999999996 23.9 29.025000000000002 5 22.725 33.800000000000004 23.0 20.474999999999998 6 20.075000000000003 35.125 25.674999999999997 19.125 7 15.299999999999999 26.85 41.449999999999996 16.400000000000002 8 16.125 26.474999999999998 33.575 23.825 9 18.025 23.3 35.25 23.425 10-14 19.465 29.215000000000003 27.805000000000003 23.515 15-19 20.375 29.07 27.88 22.675 20-24 19.575 28.199999999999996 28.53 23.695 25-29 19.580000000000002 28.675 28.415000000000003 23.330000000000002 30-34 20.23 27.74 27.839999999999996 24.19 35-39 19.744999999999997 28.349999999999998 28.294999999999998 23.61 40-44 20.05 28.299999999999997 27.985 23.665 45-49 19.439999999999998 29.725 27.560000000000002 23.275000000000002 50-54 19.68 28.7 27.74 23.880000000000003 55-59 19.31 28.83 27.750000000000004 24.11 60-64 20.165 28.395 27.939999999999998 23.5 65-69 20.29 28.904999999999998 27.71 23.095 70-74 20.04 28.785 28.144999999999996 23.03 75-79 20.69 28.754999999999995 27.275 23.28 80-84 20.335 28.544999999999998 28.235 22.884999999999998 85-89 20.794999999999998 28.22 28.54 22.445 90-94 20.53 28.165000000000003 27.284999999999997 24.02 95-99 20.925 27.6 28.615000000000002 22.86 100-104 20.585 29.37 27.43 22.615 105-109 20.630000000000003 28.599999999999998 27.034999999999997 23.735 110-114 20.36 28.17 27.88 23.59 115-119 20.79 28.244999999999997 27.295 23.669999999999998 120-124 20.115 28.235 27.905 23.745 125-129 20.61 28.065 27.145000000000003 24.18 130-134 20.485 29.345 26.229999999999997 23.94 135-139 20.665 27.565 27.565 24.205 140-144 20.48 28.51 27.355 23.655 145-149 21.54 27.985 26.71 23.765 150-151 21.15 28.075 26.0625 24.712500000000002 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 0.5 19 0.5 20 2.0 21 2.0 22 2.5 23 3.0 24 1.5 25 6.0 26 10.5 27 6.5 28 7.0 29 15.0 30 19.0 31 26.0 32 40.5 33 49.0 34 57.0 35 59.5 36 74.5 37 119.0 38 150.0 39 171.5 40 215.0 41 242.5 42 245.5 43 263.0 44 298.0 45 275.5 46 223.0 47 222.0 48 198.0 49 177.0 50 180.5 51 150.5 52 112.5 53 81.5 54 64.0 55 52.5 56 45.5 57 47.0 58 31.5 59 19.5 60 12.5 61 2.5 62 3.5 63 3.5 64 4.0 65 3.0 66 0.0 67 0.0 68 0.5 69 1.5 70 1.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.0 #Duplication Level Percentage of deduplicated Percentage of total 1 70.0 48.3 2 20.0 27.6 3 6.811594202898551 14.099999999999998 4 2.0289855072463765 5.6000000000000005 5 0.9057971014492754 3.125 6 0.036231884057971016 0.15 7 0.14492753623188406 0.7000000000000001 8 0.036231884057971016 0.2 9 0.036231884057971016 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTCAGAATAACTTAGAAGTCTGTGGTTGCTTTCCTACTGTATAACCGCC 9 0.22499999999999998 No Hit GTGGATCTGATTTAGAGGTTACAAGGCTTGGAATTGGTGCTTGGTCTTGG 8 0.2 No Hit CTGGGATGAAAAACAAAGAACAACCCAGTTCGTCAAATTCAACAAACAAA 7 0.17500000000000002 No Hit GATAAAAATTTTTGCTTGTTGGTTATAATGCCTGTTCATCATTCTTTCAA 7 0.17500000000000002 No Hit TCGCATTTGCCCATTTTGTGCACACCAATAAAACCACAAAGCCGATCAAA 7 0.17500000000000002 No Hit CCACACCAGAGGCCACAGACATGGCCAATACATAACAATGAAGAAGACAC 7 0.17500000000000002 No Hit GCATATTTGAATGAATAAGCAATCATCCTTGAATTTTACCTACAAAGATG 6 0.15 No Hit CACGCGGACCAGAAGGCAGCCCTCTCTTGCTTAAGCATCAGCACCACCAC 5 0.125 No Hit GTGTAAGTATCAACGTAGGAAAATATTCCCGGTGCATCAGTACCAGTTCT 5 0.125 No Hit CCTTGGTGCTCTGCCATTTGACTTGAAAGATCGGATTTTGGACACCACAT 5 0.125 No Hit CTGCAGCTCCATACATGGTCAAGCAAATGCTTAGGATTACGATCAGACCA 5 0.125 No Hit GTTCTGAATTTGAAGGCAATAAATGTCTAATACCCAATGTCCTTGCATGA 5 0.125 No Hit GCTTAGAGTGACGCTTAGGAGCTATTCCCTCGAAATATCCTCTTGTTTCC 5 0.125 No Hit CCTTTCTAGGCCCTATAAATCACAAGGCAAGCAAGCAAAGCAATGAACTC 5 0.125 No Hit CCGTCAACAAACACTTCGATTGCATCGTCCGGGTTCGGAAAGTGAACTCT 5 0.125 No Hit GCCAAATTGTCTATGGGAGTAACGTGCCAAGCTTGTTTCTTACCTTTTCC 5 0.125 No Hit AGCACGAACGGTAAGGGAAGAAGGGGCGGTGGAGCGGCACCGGACAATGG 5 0.125 No Hit TAAATTTTTACCACCTGACACCAATTTCCCGGCGAGATCGCACTCAGTTC 5 0.125 No Hit GTAGGTCAGATGATACTTGGAAGGTCGCCATTTTGGCATCCCATTGAAGA 5 0.125 No Hit CTTGTAGCTAGATCGCCTATGTTGAAACCTTAGGTTTTGTTCATTCCCAG 5 0.125 No Hit GGAGGAGGAAGTGTTTATGTTTCTGGACAATCACTGGGACAGGCTATATG 5 0.125 No Hit CCCAACCACAGGAAGAAAAATAGTCTGACGAGTTGATGTTTTCAGCACCA 5 0.125 No Hit CAGGAAACTCATAACTAGACAACAATTCTCTTACCTCCAACTCTACCAAT 5 0.125 No Hit CACAAGGATAGGTCGATGGTTTCTCCATTAAACTCCTACTTTCGGCCAGC 5 0.125 No Hit GTTGAATGGAAACAGGCCAAAATCCTGAGCTGAGTATTGGGGTCATTTCT 5 0.125 No Hit CTCTTCTGCAAGACGGCTTAATAGTTGAACAGCAATTGGAGGAGCACCTG 5 0.125 No Hit GCCTGTGCTGGAACAACAACAGCCAGTTTTGTAGGAATATTCTTGGGATC 5 0.125 No Hit CGAACAAGAACCTCTCTACATCATTTGGAGGGAGTCCAGACTTTGAATTT 5 0.125 No Hit CGAGACAGTGAAACATATGTTTCAAAAAACTACCTGAAATGCTGATCTTT 5 0.125 No Hit CGTCTCATCATTTTCAGGAACATCTTCATAAGAATCGATTGAAACAACTT 5 0.125 No Hit CACCAGCTGCAACCAATCCACTAATGTGTGCCATATCGGCCAACAGTATA 5 0.125 No Hit GTACCTGCAAGATTTAATTTCCAGCAAAAGATGCCCATAACCACCATCAG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0125 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.175 0.0 0.0 0.0 0.0 76-77 0.1875 0.0 0.0 0.0 0.0 78-79 0.225 0.0 0.0 0.0 0.0 80-81 0.25 0.0 0.0 0.0 0.0 82-83 0.2875 0.0 0.0 0.0 0.0 84-85 0.3375 0.0 0.0 0.0 0.0 86-87 0.475 0.0 0.0 0.0 0.0 88-89 0.55 0.0 0.0 0.0 0.0 90-91 0.725 0.0 0.0 0.0 0.0 92-93 0.775 0.0 0.0 0.0 0.0 94-95 0.8875 0.0 0.0 0.0 0.0 96-97 1.1749999999999998 0.0 0.0 0.0 0.0 98-99 1.4249999999999998 0.0 0.0 0.0 0.0 100-101 1.7625000000000002 0.0 0.0 0.0 0.0 102-103 2.025 0.0 0.0 0.0 0.0 104-105 2.25 0.0 0.0 0.0 0.0 106-107 2.6875 0.0 0.0 0.0 0.0 108-109 3.075 0.0 0.0 0.0 0.0 110-111 3.4875 0.0 0.0 0.0 0.0 112-113 3.7375 0.0 0.0 0.0 0.0 114-115 3.9625000000000004 0.0 0.0 0.0 0.0 116-117 4.25 0.0 0.0 0.0 0.0 118-119 4.675 0.0 0.0 0.0 0.0 120-121 5.125 0.0 0.0 0.0 0.0 122-123 5.65 0.0 0.0 0.0 0.0 124-125 6.15 0.0 0.0 0.0 0.0 126-127 6.4875 0.0 0.0 0.0 0.0 128-129 7.0625 0.0 0.0 0.0 0.0 130-131 7.4 0.0 0.0 0.0 0.0 132-133 7.8875 0.0 0.0 0.0 0.0 134-135 8.7875 0.0 0.0 0.0 0.0 136-137 9.275 0.0 0.0 0.0 0.0 138-139 9.425 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGTTTC 10 0.006830828 145.0 3 ATCATCA 20 0.00593511 29.0 25-29 >>END_MODULE SRR13695448 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695448_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.23775 37.0 37.0 37.0 37.0 37.0 2 36.024 37.0 37.0 37.0 37.0 37.0 3 36.073 37.0 37.0 37.0 37.0 37.0 4 36.145 37.0 37.0 37.0 37.0 37.0 5 36.209 37.0 37.0 37.0 37.0 37.0 6 36.1065 37.0 37.0 37.0 37.0 37.0 7 36.1495 37.0 37.0 37.0 37.0 37.0 8 36.3025 37.0 37.0 37.0 37.0 37.0 9 36.341 37.0 37.0 37.0 37.0 37.0 10-14 36.244 37.0 37.0 37.0 37.0 37.0 15-19 36.226299999999995 37.0 37.0 37.0 37.0 37.0 20-24 36.16495 37.0 37.0 37.0 37.0 37.0 25-29 36.16775 37.0 37.0 37.0 37.0 37.0 30-34 36.04205 37.0 37.0 37.0 37.0 37.0 35-39 36.062250000000006 37.0 37.0 37.0 37.0 37.0 40-44 36.04445 37.0 37.0 37.0 37.0 37.0 45-49 36.01915 37.0 37.0 37.0 37.0 37.0 50-54 35.93865000000001 37.0 37.0 37.0 37.0 37.0 55-59 35.96365000000001 37.0 37.0 37.0 37.0 37.0 60-64 35.89465 37.0 37.0 37.0 37.0 37.0 65-69 35.853100000000005 37.0 37.0 37.0 37.0 37.0 70-74 35.83285 37.0 37.0 37.0 37.0 37.0 75-79 35.86935 37.0 37.0 37.0 37.0 37.0 80-84 35.80575 37.0 37.0 37.0 37.0 37.0 85-89 35.682050000000004 37.0 37.0 37.0 37.0 37.0 90-94 35.70975 37.0 37.0 37.0 37.0 37.0 95-99 35.747550000000004 37.0 37.0 37.0 37.0 37.0 100-104 35.73065 37.0 37.0 37.0 37.0 37.0 105-109 35.65235 37.0 37.0 37.0 37.0 37.0 110-114 35.58095 37.0 37.0 37.0 37.0 37.0 115-119 35.50695 37.0 37.0 37.0 37.0 37.0 120-124 35.44255 37.0 37.0 37.0 37.0 37.0 125-129 35.471050000000005 37.0 37.0 37.0 37.0 37.0 130-134 35.33705 37.0 37.0 37.0 34.6 37.0 135-139 35.343650000000004 37.0 37.0 37.0 34.6 37.0 140-144 35.20195 37.0 37.0 37.0 29.8 37.0 145-149 35.10325 37.0 37.0 37.0 27.4 37.0 150-151 34.784875 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.0 17 2.0 18 2.0 19 0.0 20 0.0 21 6.0 22 5.0 23 5.0 24 6.0 25 9.0 26 6.0 27 13.0 28 12.0 29 13.0 30 32.0 31 50.0 32 83.0 33 130.0 34 250.0 35 613.0 36 2589.0 37 172.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.15627347858753 20.63611319809667 14.450288004007012 32.75732531930879 2 29.425 25.974999999999998 30.275000000000002 14.325 3 18.675 30.425 27.700000000000003 23.200000000000003 4 23.525 34.849999999999994 22.75 18.875 5 24.425 38.275 20.1 17.2 6 20.549999999999997 38.925 22.975 17.549999999999997 7 19.8 21.5 39.775 18.925 8 21.825 24.325 29.2 24.65 9 21.25 25.074999999999996 31.574999999999996 22.1 10-14 22.189999999999998 30.380000000000003 27.08 20.349999999999998 15-19 22.220000000000002 28.725 28.199999999999996 20.855 20-24 22.23555888972243 28.592148037009252 28.377094273568392 20.795198799699925 25-29 22.2655663915979 29.037259314828706 28.652163040760193 20.045011252813204 30-34 22.310577644411104 28.887221805451365 28.477119279819956 20.32508127031758 35-39 23.030757689422355 28.42710677669417 27.47186796699175 21.070267566891722 40-44 22.34058514628657 27.541885471367845 29.08727181795449 21.030257564391096 45-49 22.630657664416105 28.02700675168792 28.237059264816207 21.10527631907977 50-54 22.43060765191298 29.197299324831206 27.156789197299325 21.21530382595649 55-59 22.23555888972243 28.612153038259564 28.06201550387597 21.090272568142034 60-64 22.455613903475868 28.00700175043761 28.22705676419105 21.310327581895475 65-69 22.494498899779956 27.580516103220642 28.735747149429887 21.189237847569515 70-74 22.500625156289072 28.212053013253314 27.881970492623154 21.405351337834457 75-79 22.035508877219304 27.976994248562143 28.52213053263316 21.465366341585394 80-84 23.645911477869465 28.417104276069015 27.60190047511878 20.335083770942735 85-89 23.365841460365093 28.41210302575644 27.22680670167542 20.99524881220305 90-94 22.685671417854465 28.33708427106777 28.54713678419605 20.43010752688172 95-99 23.540885221305327 27.836959239809957 28.037009252313077 20.585146286571643 100-104 22.40060015003751 28.267066766691674 27.326831707926978 22.005501375343837 105-109 22.845711427856966 28.24206051512878 28.24206051512878 20.67016754188547 110-114 23.485871467866968 28.447111777944485 27.121780445111277 20.94523630907727 115-119 23.440860215053764 28.557139284821204 27.84196049012253 20.1600400100025 120-124 24.086021505376344 28.052013003250813 27.561890472618156 20.300075018754686 125-129 23.905976494123532 27.7569392348087 28.477119279819956 19.85996499124781 130-134 24.44855699494823 27.984794678137348 27.484619616865903 20.082028710048515 135-139 24.831207801950487 27.571892973243312 27.481870467616904 20.115028757189297 140-144 25.331332833208304 28.91722930732683 26.776694173543387 18.97474368592148 145-149 26.03650912728182 27.981995498874717 26.156539134783696 19.824956239059766 150-151 26.559959984994375 27.42278354382894 27.160185069401027 18.857071401775666 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 1.0 8 1.0 9 0.0 10 0.0 11 0.5 12 0.5 13 0.5 14 0.5 15 0.5 16 0.5 17 0.5 18 1.0 19 0.5 20 0.0 21 0.0 22 0.5 23 2.5 24 3.5 25 3.5 26 7.5 27 8.5 28 13.0 29 20.5 30 24.0 31 27.5 32 34.0 33 41.0 34 55.0 35 79.5 36 98.0 37 116.0 38 150.5 39 185.0 40 199.5 41 219.0 42 256.0 43 274.5 44 278.0 45 297.5 46 282.0 47 235.5 48 203.0 49 172.5 50 148.5 51 123.5 52 95.5 53 82.5 54 75.0 55 52.5 56 32.5 57 22.0 58 14.5 59 15.0 60 13.0 61 8.5 62 4.0 63 2.0 64 3.5 65 2.5 66 1.5 67 1.0 68 1.0 69 1.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.5 83 0.5 84 0.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.17500000000000002 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.025 25-29 0.025 30-34 0.025 35-39 0.025 40-44 0.025 45-49 0.025 50-54 0.025 55-59 0.025 60-64 0.025 65-69 0.02 70-74 0.025 75-79 0.025 80-84 0.025 85-89 0.025 90-94 0.025 95-99 0.025 100-104 0.025 105-109 0.025 110-114 0.025 115-119 0.025 120-124 0.025 125-129 0.025 130-134 0.034999999999999996 135-139 0.025 140-144 0.025 145-149 0.025 150-151 0.0375 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.65 #Duplication Level Percentage of deduplicated Percentage of total 1 71.14142139267767 49.55 2 19.059583632447954 26.55 3 6.532663316582915 13.65 4 2.189519023689878 6.1 5 0.7896625987078248 2.75 6 0.07178750897343862 0.3 7 0.17946877243359655 0.8750000000000001 8 0.0 0.0 9 0.03589375448671931 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTAGTTCGCTGATTGGAATTAGAACTGTGTACATAATTGTGTTTTTGGT 9 0.22499999999999998 No Hit AGAGGGAGAAGTGAAGACCCTTTCAATCTCTCAGTGAAAAAGGAGAGAAG 7 0.17500000000000002 No Hit CAGAGATGGAAATGATGGCTGGGAGGATAAAAAATGTGAGACAGAAGCTA 7 0.17500000000000002 No Hit TTCATTTTCATATCATCCCATTCGAAGTTGTTCCAGTAGCTGGTATCGCC 7 0.17500000000000002 No Hit ATAAAAATGCACTCAACCATCCAATATTTGAAAGTTTGCTTTTTGATCAG 7 0.17500000000000002 No Hit GTGGATGGTTACAGCCCTATCTACAACGCTGATGAATGGTCCCCATCCGG 7 0.17500000000000002 No Hit CTCAAAAAAAATCTTAACTGCACCCGCTCATCCAGCAATGGCAGCAGCAA 6 0.15 No Hit TGGAGAAATATTGGAGATTGTTGCTGGTGCCGATGCTTTAACCTAATCAT 6 0.15 No Hit GTAGAAGTTTCATCAACAACCACTGTAAGTTCTTGCAAACATATTTGAAA 5 0.125 No Hit TGGAGATGGACGGATTGTCTGGTCTCTCTTATTGAACTCTCTACGTCAAA 5 0.125 No Hit GTTTGGTTTCCATTCCTCAAAAGCATTCTAACCTTAGCATTTGTCAGTCA 5 0.125 No Hit GCCAAATCTTCTTTCTTTTTCTTTTTGCTTGGTTGCACTTTATTGGGAAA 5 0.125 No Hit CAGAAAAGAAGTGGGCTACTTGATAGCTTTTGAAGAAAAGGAAAATTAAA 5 0.125 No Hit AGCTAGAGAATTGTGTGGATGGGAGGAGGTATCTGAGCTACTTGCACTTT 5 0.125 No Hit GTTGATTGCCCTGGTCATGCCGATTATGTTAAGAACATGATTACTGGTGC 5 0.125 No Hit AAAGAACATCAATATGGCTCCTAAACTTTCCTGTCTTTTGTTAGCTATCC 5 0.125 No Hit GTTAGGCTTGTTAGCTTCAAGGCTCATAAGACCCCAAAAAACCGCCGCCC 5 0.125 No Hit CTCTTATGGCAGTTATGGTGGAGCATATAGAAGGTCAAAGAGACCTTATA 5 0.125 No Hit GAAACATTTTCCATCATGGTTCTGATGGCCATTTTCACAACTTTTATCAC 5 0.125 No Hit CAAGCCCCTCTTAGACAGAATAGTGTCGGTGGTATTCTTTGCAAGGGACC 5 0.125 No Hit TCTTTATCTGGATCTCCATCTAATTTTCAAGTCTACACTGCATTGTTAAA 5 0.125 No Hit ACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAAT 5 0.125 No Hit TCCAACGCTCTCTCCTTCTCACCTTCAAGACTTGCCAGGTTTCACACGTA 5 0.125 No Hit CCCGGCCTTTCCTTCTCAGTGGTCTCCTGATTTTGTTTTTTTAGCTTGAA 5 0.125 No Hit AAAGAAACTATTTGATTTGTGCTTCTGCTAGAGAGAGAGAGAGAGACTTC 5 0.125 No Hit GCAGATAAGATAGCAGACACAAATTGTTATTATAGGCTCTATCTTGCACT 5 0.125 No Hit AGACAAGCCAACTTACCAAGTGGTTCAACCAATCAATGGCGATCCCTTCA 5 0.125 No Hit TTTGAGGATACAGAGGGTATATGGATCAGTGCGTTGCTCCTGGCTTTCTT 5 0.125 No Hit AACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAA 5 0.125 No Hit GACCATGTTTGGGTGGCTTTTAATTGTTTCTGTGTGATAGTTTAAGGCCC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0125 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.175 0.0 0.0 0.0 0.0 76-77 0.1875 0.0 0.0 0.0 0.0 78-79 0.225 0.0 0.0 0.0 0.0 80-81 0.25 0.0 0.0 0.0 0.0 82-83 0.2875 0.0 0.0 0.0 0.0 84-85 0.3625 0.0 0.0 0.0 0.0 86-87 0.5 0.0 0.0 0.0 0.0 88-89 0.575 0.0 0.0 0.0 0.0 90-91 0.75 0.0 0.0 0.0 0.0 92-93 0.8 0.0 0.0 0.0 0.0 94-95 0.9125 0.0 0.0 0.0 0.0 96-97 1.2000000000000002 0.0 0.0 0.0 0.0 98-99 1.4500000000000002 0.0 0.0 0.0 0.0 100-101 1.7875 0.0 0.0 0.0 0.0 102-103 2.05 0.0 0.0 0.0 0.0 104-105 2.275 0.0 0.0 0.0 0.0 106-107 2.7125000000000004 0.0 0.0 0.0 0.0 108-109 3.1500000000000004 0.0 0.0 0.0 0.0 110-111 3.575 0.0 0.0 0.0 0.0 112-113 3.8499999999999996 0.0 0.0 0.0 0.0 114-115 4.0875 0.0 0.0 0.0 0.0 116-117 4.375 0.0 0.0 0.0 0.0 118-119 4.8 0.0 0.0 0.0 0.0 120-121 5.237500000000001 0.0 0.0 0.0 0.0 122-123 5.800000000000001 0.0 0.0 0.0 0.0 124-125 6.325 0.0 0.0 0.0 0.0 126-127 6.6875 0.0 0.0 0.0 0.0 128-129 7.25 0.0 0.0 0.0 0.0 130-131 7.55 0.0 0.0 0.0 0.0 132-133 8.0375 0.0 0.0 0.0 0.0 134-135 8.8875 0.0 0.0 0.0 0.0 136-137 9.3875 0.0 0.0 0.0 0.0 138-139 9.5125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAGCTT 10 0.006830828 145.0 145 >>END_MODULE Read 904283 spots for SRR13695448.sra Written 904283 spots for SRR13695448.sra Read 904283 spots for SRR13695448.sra Written 904283 spots for SRR13695448.sra Read 904283 spots for SRR13695448.sra Written 904283 spots for SRR13695448.sra Read 904283 spots for SRR13695448.sra Written 904283 spots for SRR13695448.sra Read 904283 spots for SRR13695448.sra Written 904283 spots for SRR13695448.sra Read 904283 spots for SRR13695448.sra Written 904283 spots for SRR13695448.sra Read 904283 spots for SRR13695448.sra Written 904283 spots for SRR13695448.sra Read 904283 spots for SRR13695448.sra Written 904283 spots for SRR13695448.sra Read 904283 spots for SRR13695448.sra Written 904283 spots for SRR13695448.sra Read 904288 spots for SRR13695448.sra Written 904288 spots for SRR13695448.sra Read 904283 spots for SRR13695448.sra Written 904283 spots for SRR13695448.sra Read 904283 spots for SRR13695448.sra Written 904283 spots for SRR13695448.sra Read 904283 spots for SRR13695448.sra Written 904283 spots for SRR13695448.sra Read 904283 spots for SRR13695448.sra Written 904283 spots for SRR13695448.sra Read 904283 spots for SRR13695448.sra Written 904283 spots for SRR13695448.sra Read 904283 spots for SRR13695448.sra Written 904283 spots for SRR13695448.sra Read 904283 spots for SRR13695448.sra Written 904283 spots for SRR13695448.sra Read 904283 spots for SRR13695448.sra Written 904283 spots for SRR13695448.sra Read 904283 spots for SRR13695448.sra Written 904283 spots for SRR13695448.sra Read 904283 spots for SRR13695448.sra Written 904283 spots for SRR13695448.sra SRR ids: ['SRR13695448.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_rfwyq7le SRR13695448.sra spots: 18085665 blocks: [[1, 904283], [904284, 1808566], [1808567, 2712849], [2712850, 3617132], [3617133, 4521415], [4521416, 5425698], [5425699, 6329981], [6329982, 7234264], [7234265, 8138547], [8138548, 9042830], [9042831, 9947113], [9947114, 10851396], [10851397, 11755679], [11755680, 12659962], [12659963, 13564245], [13564246, 14468528], [14468529, 15372811], [15372812, 16277094], [16277095, 17181377], [17181378, 18085665]] SRR13695448 file size 6124599 SRR13695448 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695448 SRR13695448_1.fastq SRR13695448_2.fastq Input file: SRR13695448_1.fastq Paired file: SRR13695448_2.fastq trimmed: SRR13695448-trimmed-pair1.fastq, SRR13695448-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 03:44:34 2025 >> started Wed Feb 12 03:44:52 2025 >> done (18.424s) 18085665 read pairs processed; of these: 125 ( 0.00%) short read pairs filtered out after trimming by size control 1495 ( 0.01%) empty read pairs filtered out after trimming by size control 18084045 (99.99%) read pairs available; of these: 2048322 (11.33%) trimmed read pairs available after processing 16035723 (88.67%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 5 0.00% 20 4 0.00% 21 6 0.00% 22 5 0.00% 23 1 0.00% 24 2 0.00% 25 4 0.00% 26 5 0.00% 27 1 0.00% 28 5 0.00% 29 4 0.00% 30 8 0.00% 31 0 0.00% 32 5 0.00% 33 7 0.00% 34 5 0.00% 35 7 0.00% 36 16 0.00% 37 22 0.00% 38 17 0.00% 39 18 0.00% 40 17 0.00% 41 22 0.00% 42 37 0.00% 43 25 0.00% 44 34 0.00% 45 26 0.00% 46 35 0.00% 47 49 0.00% 48 61 0.00% 49 86 0.00% 50 121 0.00% 51 102 0.00% 52 83 0.00% 53 137 0.00% 54 134 0.00% 55 139 0.00% 56 175 0.00% 57 163 0.00% 58 232 0.00% 59 218 0.00% 60 285 0.00% 61 355 0.00% 62 411 0.00% 63 460 0.00% 64 559 0.00% 65 544 0.00% 66 640 0.00% 67 702 0.00% 68 799 0.00% 69 921 0.01% 70 1081 0.01% 71 1239 0.01% 72 1448 0.01% 73 1641 0.01% 74 1668 0.01% 75 1940 0.01% 76 2078 0.01% 77 2305 0.01% 78 2637 0.01% 79 2911 0.02% 80 3297 0.02% 81 3737 0.02% 82 4332 0.02% 83 4705 0.03% 84 5388 0.03% 85 5691 0.03% 86 6018 0.03% 87 6615 0.04% 88 7071 0.04% 89 7797 0.04% 90 7895 0.04% 91 8763 0.05% 92 9502 0.05% 93 10018 0.06% 94 11051 0.06% 95 11770 0.07% 96 12673 0.07% 97 13200 0.07% 98 13594 0.08% 99 14461 0.08% 100 15421 0.09% 101 15955 0.09% 102 16937 0.09% 103 17520 0.10% 104 18332 0.10% 105 19692 0.11% 106 20512 0.11% 107 20946 0.12% 108 21491 0.12% 109 22263 0.12% 110 22962 0.13% 111 23940 0.13% 112 24726 0.14% 113 25351 0.14% 114 26498 0.15% 115 27487 0.15% 116 28823 0.16% 117 29708 0.16% 118 30461 0.17% 119 31221 0.17% 120 32161 0.18% 121 32981 0.18% 122 34282 0.19% 123 34394 0.19% 124 35417 0.20% 125 36767 0.20% 126 37760 0.21% 127 38327 0.21% 128 39085 0.22% 129 39231 0.22% 130 41365 0.23% 131 41869 0.23% 132 42507 0.24% 133 42888 0.24% 134 44354 0.25% 135 45080 0.25% 136 46401 0.26% 137 47004 0.26% 138 47959 0.27% 139 48630 0.27% 140 49218 0.27% 141 49908 0.28% 142 50682 0.28% 143 50721 0.28% 144 52307 0.29% 145 53070 0.29% 146 53384 0.30% 147 54860 0.30% 148 56979 0.32% 149 56501 0.31% 150 57739 0.32% 151 16035723 88.67% 18084045 reads passed initial QC criterion=sequence-density sequence-density=0.31 sequence-density-rank=1 fanout-score=2.48 fanout-score-rank=16 prefix-density=0.34 prefix-fanout=2.3 sequence=TTAGCCTTTCTGGT criterion=fanout-score sequence-density=0.01 sequence-density-rank=27 fanout-score=432.16 fanout-score-rank=1 prefix-density=0.27 prefix-fanout=17.6 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGAGTATGAATGTGTTCTCTGT criterion=sequence-density sequence-density=0.45 sequence-density-rank=1 fanout-score=2.07 fanout-score-rank=30 prefix-density=0.45 prefix-fanout=2.1 sequence=TGTAAGAGATGGCTTCCTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=69.68 fanout-score-rank=1 prefix-density=0.12 prefix-fanout=3.2 sequence=AGGAACTCAAACGCATAGCTTCCTACAAATACCCCAGCTAGCCAATACTCTCAACCACTAAACCCAAAGCTCAAAAAAAATCTTAACTGCACCCGCTCATCCAGCAATGGCAGCAGCAACAATGGCCCTCTCGTCCCCTTCGCTAGCCGGAAAGGCGGTGAAGCTCAACCCCTCCTCCTCTGAGATCATGGGCAATGGCCGTGTCTCCATGAGGAAAACCACCAAGCCTGTTCCCTCCGGGAGCCCATGGTACGGACCAGACCGTGTTAAATACTTGGGCCCGTTCTCTGGTGAGCCCCCATCCTACTTGACTGGTGAGTTCCCTGGTGACTACGGCTGGGACACTGCTGGCCTCTCTGCTGACCCAGAGACCTTTGCCAAGAACCGTGAGCTTGAAGTCATCCATTCCAGGTGGGCCATGCTTGGAGCTCTTGGATGCGTCTTCCCCGAGCTCTTGTCCCGCAACGGTGTCAAGTTCGGCGAGGCTGTATGGTTCAAGGCTGGAGCCCAG SRR13695448 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 03:45:33 Started mapping on | Feb 12 03:45:33 Finished on | Feb 12 03:47:21 Mapping speed, Million of reads per hour | 602.80 Number of input reads | 18084045 Average input read length | 296 UNIQUE READS: Uniquely mapped reads number | 16952322 Uniquely mapped reads % | 93.74% Average mapped length | 294.90 Number of splices: Total | 16898943 Number of splices: Annotated (sjdb) | 16500632 Number of splices: GT/AG | 16557493 Number of splices: GC/AG | 265135 Number of splices: AT/AC | 10162 Number of splices: Non-canonical | 66153 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.03% Deletion average length | 2.89 Insertion rate per base | 0.02% Insertion average length | 2.40 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 385894 % of reads mapped to multiple loci | 2.13% Number of reads mapped to too many loci | 86867 % of reads mapped to too many loci | 0.48% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.52% % of reads unmapped: other | 0.13% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 746057 746057 746057 N_multimapping 385894 385894 385894 N_noFeature 692589 16490320 989331 N_ambiguous 263711 1726 97204 UnstrandedReadsAssigned:15996022 PositiveStrandReadsAssigned:460276 NegativeStrandReadsAssigned:15865787 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695448 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695448-trimmed-pair1.fastq SRR13695448-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 18,084,045 reads, 15,896,983 reads pseudoaligned [quant] estimated average fragment length: 256.887 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,095 rounds 52401 SRR13695448.ke.tsv 34699 SRR13695448.se.tsv 87100 total ==> SRR13695448.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1762.11 558 18.6841 Potri.005G024800.1.v4.1 1035 779.113 228 17.2666 Potri.004G059700.1.v4.1 961 705.245 20 1.67325 Potri.007G009000.2.v4.1 1416 1160.11 0 0 Potri.003G141000.2.v4.1 2943 2687.11 916.492 20.124 Potri.016G087400.1.v4.1 270 85.5563 714 492.4 Potri.015G069301.1.v4.1 564 320.539 0 0 Potri.010G195200.1.v4.1 1773 1517.11 84 3.26688 Potri.012G127500.1.v4.1 977 721.165 52 4.25442 ==> SRR13695448.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 158 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 216 Potri.001G212900.v4.1 8 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 23 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 8 SRR13695448 completed mapping pipeline successfully