Starting /dee2/code/volunteer_pipeline.sh SRR13695449 current disk space = 3048973217792 free memory = 1579241164 SRR13695449 SRAfilesize 6cd98ef421f9b7ab516d628f2131ef03 SRR13695449.sra SRR13695449.sra file validated SRR13695449 is paired end SRR13695449 is conventional basespace SRR13695449 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695449_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.6625 37.0 37.0 37.0 37.0 37.0 2 36.29925 37.0 37.0 37.0 37.0 37.0 3 36.587 37.0 37.0 37.0 37.0 37.0 4 36.6235 37.0 37.0 37.0 37.0 37.0 5 36.567 37.0 37.0 37.0 37.0 37.0 6 36.5485 37.0 37.0 37.0 37.0 37.0 7 36.405 37.0 37.0 37.0 37.0 37.0 8 36.5255 37.0 37.0 37.0 37.0 37.0 9 36.552 37.0 37.0 37.0 37.0 37.0 10-14 36.5531 37.0 37.0 37.0 37.0 37.0 15-19 36.487300000000005 37.0 37.0 37.0 37.0 37.0 20-24 36.5473 37.0 37.0 37.0 37.0 37.0 25-29 36.4967 37.0 37.0 37.0 37.0 37.0 30-34 36.4657 37.0 37.0 37.0 37.0 37.0 35-39 36.4496 37.0 37.0 37.0 37.0 37.0 40-44 36.411500000000004 37.0 37.0 37.0 37.0 37.0 45-49 36.406400000000005 37.0 37.0 37.0 37.0 37.0 50-54 36.352999999999994 37.0 37.0 37.0 37.0 37.0 55-59 36.3224 37.0 37.0 37.0 37.0 37.0 60-64 36.3515 37.0 37.0 37.0 37.0 37.0 65-69 36.2943 37.0 37.0 37.0 37.0 37.0 70-74 36.312 37.0 37.0 37.0 37.0 37.0 75-79 36.215900000000005 37.0 37.0 37.0 37.0 37.0 80-84 36.2102 37.0 37.0 37.0 37.0 37.0 85-89 36.202999999999996 37.0 37.0 37.0 37.0 37.0 90-94 36.1682 37.0 37.0 37.0 37.0 37.0 95-99 36.093500000000006 37.0 37.0 37.0 37.0 37.0 100-104 36.055600000000005 37.0 37.0 37.0 37.0 37.0 105-109 36.0785 37.0 37.0 37.0 37.0 37.0 110-114 36.044000000000004 37.0 37.0 37.0 37.0 37.0 115-119 36.0585 37.0 37.0 37.0 37.0 37.0 120-124 36.0 37.0 37.0 37.0 37.0 37.0 125-129 35.9585 37.0 37.0 37.0 37.0 37.0 130-134 35.903200000000005 37.0 37.0 37.0 37.0 37.0 135-139 35.8492 37.0 37.0 37.0 37.0 37.0 140-144 35.7273 37.0 37.0 37.0 37.0 37.0 145-149 35.649 37.0 37.0 37.0 37.0 37.0 150-151 35.3635 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 3.0 25 2.0 26 7.0 27 13.0 28 23.0 29 15.0 30 25.0 31 33.0 32 44.0 33 90.0 34 138.0 35 313.0 36 2893.0 37 401.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.574999999999996 13.475000000000001 7.475 40.475 2 19.63791802866482 13.879808901181795 36.962534573799346 29.519738496354037 3 19.8 18.075 27.075 35.05 4 23.325000000000003 23.525 23.9 29.25 5 22.975 31.8 24.575 20.65 6 20.275000000000002 33.475 24.7 21.55 7 16.900000000000002 27.325 38.35 17.424999999999997 8 17.7 26.525 31.35 24.425 9 17.0 24.825 34.525 23.65 10-14 19.925 29.675 28.055000000000003 22.345000000000002 15-19 19.35 28.65 28.155 23.845 20-24 19.375 28.444999999999997 28.285 23.895 25-29 19.52 28.96 28.005000000000003 23.515 30-34 19.42 28.59 28.17 23.82 35-39 20.155 29.060000000000002 27.405 23.380000000000003 40-44 20.46 28.410000000000004 27.400000000000002 23.73 45-49 19.97 28.64 27.450000000000003 23.94 50-54 18.825 29.054999999999996 27.54 24.58 55-59 20.21 28.825 27.339999999999996 23.625 60-64 19.78 28.815 27.715 23.69 65-69 20.23 28.415000000000003 27.075 24.279999999999998 70-74 20.205000000000002 28.32 27.544999999999998 23.93 75-79 19.634999999999998 28.095 29.39 22.88 80-84 19.825 28.42 27.650000000000002 24.104999999999997 85-89 19.855 28.12 28.1 23.925 90-94 20.419999999999998 27.52 28.025 24.035 95-99 20.005 29.270000000000003 27.465 23.26 100-104 20.535 28.225 27.245 23.995 105-109 20.36 28.439999999999998 27.605 23.595 110-114 20.665 27.455000000000002 28.59 23.29 115-119 20.525 27.66 27.750000000000004 24.065 120-124 21.560000000000002 27.91 26.52 24.01 125-129 21.215 28.910000000000004 26.529999999999998 23.345 130-134 21.785 27.1 27.11 24.005000000000003 135-139 22.335 27.875 26.0 23.79 140-144 21.55 27.500000000000004 26.435 24.515 145-149 20.965 27.045 26.71 25.28 150-151 21.762500000000003 27.212500000000002 27.0125 24.0125 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1.0 1 0.5 2 0.0 3 0.5 4 0.5 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 1.0 22 1.0 23 1.0 24 4.0 25 5.5 26 5.5 27 5.5 28 13.0 29 20.5 30 23.0 31 27.5 32 27.5 33 43.5 34 65.5 35 83.0 36 99.0 37 114.5 38 149.5 39 191.0 40 206.0 41 211.5 42 216.0 43 213.0 44 235.0 45 253.5 46 251.0 47 217.0 48 202.5 49 214.5 50 186.5 51 153.5 52 119.0 53 97.0 54 91.5 55 72.5 56 54.0 57 33.0 58 21.0 59 18.0 60 8.5 61 11.0 62 10.5 63 7.0 64 5.0 65 0.5 66 0.0 67 2.5 68 2.5 69 0.0 70 1.0 71 1.0 72 0.5 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.575 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.9 #Duplication Level Percentage of deduplicated Percentage of total 1 68.51289833080425 45.15 2 19.385432473444613 25.55 3 7.473444613050076 14.774999999999999 4 2.7693474962063735 7.3 5 0.9484066767830045 3.125 6 0.5690440060698027 2.25 7 0.07587253414264036 0.35000000000000003 8 0.18968133535660092 1.0 9 0.03793626707132018 0.22499999999999998 >10 0.03793626707132018 0.27499999999999997 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGTTAGCGCCGCAC 11 0.27499999999999997 No Hit GCCTGTTGAGGTTGGTGTAGGTGGGTCGCTCAATGTCAAGAGAACGCTTG 9 0.22499999999999998 No Hit ATCTACAAGAAGGTTTCCAAACAATCCAGACAAAATTGCCACAAGACCAT 8 0.2 No Hit GCCTGCCACTTCCTGATATGCACAACCTAAGGTCCACCATGGTGATTTTA 8 0.2 No Hit CACATTTCAATGACATAGAAGGGGAAAAAAACACAGCAAACAGTTCTGCC 8 0.2 No Hit GATGACAGTTAGTTCTTGAAGTTTGGAACCCAACAAGTGCAACCAGAACA 8 0.2 No Hit TGTATGGATCTGCCCAGTAGCTGTATGTCCCGGCCGGTGGGATGACACCA 8 0.2 No Hit AAGGAGAGCACCTGCATTGTCAAACTCCCCACCATAGTTTGGAGCTGAGA 7 0.17500000000000002 No Hit CAGAAATCTAAGGCTTACTCCAAGAGGTTTCAAGTCAAGTTTAAGAGAAG 7 0.17500000000000002 No Hit GGGAACATCACAGTTGTGAGATGAGGGAACAATATCTTCAAGCTTTTTCC 6 0.15 No Hit CCCTACTCTTCCTTAACTTTCTAAACCCCACATGGACTTTCCTGCTTGAA 6 0.15 No Hit AGCATAATATCAAACACAAGCATGGGTGAACCATGAACTCCAGTGAATAT 6 0.15 No Hit GAAGCCTCCGCTGCTACTACCTCTGTTTTCTTCTCTTCTTCAACTACTAT 6 0.15 No Hit CCTTAACCATCTTCTCAAGCAATACACAGTTTAACCTTTCTTCTTCCTCT 6 0.15 No Hit CTCCCTCTTTAAGTACATACTGCTCAGGTGACAGTTCAAATAATTTACCA 6 0.15 No Hit CTTGCACTTGCTCCTTCACCCGAACCTTCTTGGTAGACCTGGCGGGATTC 6 0.15 No Hit GTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCAC 6 0.15 No Hit GGCCAAGGGGGCAAGACAGTTTGTAGTGCAGCTAGCATTGGAAACAATGT 6 0.15 No Hit CTCCAGCCACGTCACCTCCGCGACAAGGTAAGGTTTCGTACGGACTATAT 6 0.15 No Hit GCAACAATATAATTTGATTATCCTTAGAAAGTGCTTCTCCTTACACACCC 6 0.15 No Hit CCTCCTTGACAGGTATACCATTGATTCTAGCATTTAGAAAGTCCGGAACC 6 0.15 No Hit CCAGAATACCCTTTTATAAATTCCCATGCTTCTTCCTTGCTGGCTGGCTT 6 0.15 No Hit GCCAAAATCCTGATGAGTTCCTCATCACTGTAAGCCTTATTTGAAATCTT 6 0.15 No Hit ATAGGATACACAGTCTTCTCACAAGCCTTGCATTTCTGTTGGGTACCAGT 6 0.15 No Hit CCAAGGCTAAGACACAGCCAATGCAGTAGATAATTTAATTCATCCAAATG 5 0.125 No Hit GGCGGTAATAAAGCTGATGCACTGCACTTGACGCGTGTTGTCGAATCCGA 5 0.125 No Hit CATCTCTTGTCTCCAGCTTGTGCCTTGCAAATGTAAAGCTTACCATCTTT 5 0.125 No Hit CTCTACAATGCGGCAAAGTAATCCTTGCTCTGTCTTGGGTCAGGTTTCAT 5 0.125 No Hit CTGGTGTTTGGACTTCCCATTTATTTGCATATTCTCAACTATACCACCAG 5 0.125 No Hit TCCAGAGATATGTGTTCTGGCTTCATCAGTGCAGGGACTCAACTGATGCT 5 0.125 No Hit CTCCAACAGAACATTTTATAATGGCATTGCATTTAAACACAGATTCTAAA 5 0.125 No Hit CTCCATCATAGGCCTTCCCACGTACCAGTATTTTCGCTTTGGTGCCTGCC 5 0.125 No Hit GGGATTTTACAAGACATAATAGCTCATCTACTCTTCATCCTCATCGGTCA 5 0.125 No Hit GTGCTTCTATCTTATCCATAGCCAAAGGGGCAAGATCTTCTAGAGATACG 5 0.125 No Hit TCATTTCCAATCTCACAAAATATAAGCACATACTAAACCATAAAGTATAA 5 0.125 No Hit GTTTGGGGAGGTGGGCTGCGAGCTCGGGCAGGTCGAGGAGCTGGGCGGGC 5 0.125 No Hit ACACCACTGGCTTCAATCTTGAAAGTCCTCCTTGAAAGTGTTGGGAGGCC 5 0.125 No Hit ATCCTTTCTAGTCACACGGGCAGCAGGTATTCCACAAGCATCTGCAAACT 5 0.125 No Hit CGGCCATTAACATACAATGTCCAAACATTGTCACCACCTACATGACTATG 5 0.125 No Hit GTTTAGGACATGATCACTGCAAGCTATGCTGGTATTCGCGTACCTTTATT 5 0.125 No Hit CCCCAACGGTCCTTAAGATTTTTTCCAAACGACATGTTAATATAATTTCT 5 0.125 No Hit CCTCCTGTGCCTTCAGGGTCTGAAAGTCCGAGTGGGTCGAATCCGAAGTC 5 0.125 No Hit TTTCATTTCTCTAGAATCAGCTCTAGTCTGTTGGGCTCTGATCACAGGCA 5 0.125 No Hit GTTGAACTAAGAGTTTGGGCAAGACTGCCTTTTTCATTGAATTGAACTGG 5 0.125 No Hit CATAGCCCTGGTGCTTTGAGCTCACAGCTTTTACCACTAGATAATGGACC 5 0.125 No Hit TGACTTTCGACTTCAAAAACAACTGTCCATACACAGTCTGGCCAGGAACT 5 0.125 No Hit GACCTCTCTTCCATTTATGCAGGAATGGCTTCGACGAGTCTTGCAAAGTA 5 0.125 No Hit TTGCTTTGTGCGTGGATCAAGCTTGGGTTGCCCAAGTAGTCAAGTCCACC 5 0.125 No Hit CTTCACTGAGGATACATGAGATGATGACACGAGCAAGGCTGCGAAACTTA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.1125 0.0 0.0 0.0 0.0 78-79 0.16249999999999998 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.23750000000000002 0.0 0.0 0.0 0.0 84-85 0.2875 0.0 0.0 0.0 0.0 86-87 0.4375 0.0 0.0 0.0 0.0 88-89 0.5625 0.0 0.0 0.0 0.0 90-91 0.7 0.0 0.0 0.0 0.0 92-93 1.0125000000000002 0.0 0.0 0.0 0.0 94-95 1.2375 0.0 0.0 0.0 0.0 96-97 1.5625 0.0 0.0 0.0 0.0 98-99 1.7374999999999998 0.0 0.0 0.0 0.0 100-101 2.0625 0.025 0.0 0.0 0.0 102-103 2.4 0.025 0.0 0.0 0.0 104-105 2.65 0.025 0.0 0.0 0.0 106-107 3.0 0.025 0.0 0.0 0.0 108-109 3.4875 0.025 0.0 0.0 0.0 110-111 3.9125 0.025 0.0 0.0 0.0 112-113 4.325 0.025 0.0 0.0 0.0 114-115 4.775 0.025 0.0 0.0 0.0 116-117 5.4375 0.025 0.0 0.0 0.0 118-119 6.1875 0.025 0.0 0.0 0.0 120-121 6.875 0.025 0.0 0.0 0.0 122-123 7.3875 0.025 0.0 0.0 0.0 124-125 7.9125 0.025 0.0 0.0 0.0 126-127 8.6625 0.025 0.0 0.0 0.0 128-129 9.7 0.025 0.0 0.0 0.0 130-131 10.6 0.025 0.0 0.0 0.0 132-133 11.5625 0.025 0.0 0.0 0.0 134-135 12.3125 0.025 0.0 0.0 0.0 136-137 13.125 0.025 0.0 0.0 0.0 138-139 14.075 0.025 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTTTGCA 10 0.006830828 145.0 6 TATATCA 10 0.006830828 145.0 145 TTGTAGC 10 0.006830828 145.0 8 TTCAAGT 10 0.006830828 145.0 2 AGTAGAA 10 0.006830828 145.0 7 GTTTCAA 10 0.006830828 145.0 7 AAGTTTC 10 0.006830828 145.0 5 TATGGTG 10 0.006830828 145.0 9 >>END_MODULE SRR13695449 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695449_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.231 37.0 37.0 37.0 37.0 37.0 2 36.2635 37.0 37.0 37.0 37.0 37.0 3 36.2765 37.0 37.0 37.0 37.0 37.0 4 36.293 37.0 37.0 37.0 37.0 37.0 5 36.4215 37.0 37.0 37.0 37.0 37.0 6 36.224 37.0 37.0 37.0 37.0 37.0 7 36.3435 37.0 37.0 37.0 37.0 37.0 8 36.2625 37.0 37.0 37.0 37.0 37.0 9 36.312 37.0 37.0 37.0 37.0 37.0 10-14 36.322199999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.292699999999996 37.0 37.0 37.0 37.0 37.0 20-24 36.243849999999995 37.0 37.0 37.0 37.0 37.0 25-29 36.1787 37.0 37.0 37.0 37.0 37.0 30-34 36.1476 37.0 37.0 37.0 37.0 37.0 35-39 36.118300000000005 37.0 37.0 37.0 37.0 37.0 40-44 36.0972 37.0 37.0 37.0 37.0 37.0 45-49 36.1 37.0 37.0 37.0 37.0 37.0 50-54 36.02919999999999 37.0 37.0 37.0 37.0 37.0 55-59 36.0917 37.0 37.0 37.0 37.0 37.0 60-64 35.9578 37.0 37.0 37.0 37.0 37.0 65-69 35.989 37.0 37.0 37.0 37.0 37.0 70-74 35.8802 37.0 37.0 37.0 37.0 37.0 75-79 35.9507 37.0 37.0 37.0 37.0 37.0 80-84 35.9294 37.0 37.0 37.0 37.0 37.0 85-89 35.7903 37.0 37.0 37.0 37.0 37.0 90-94 35.7844 37.0 37.0 37.0 37.0 37.0 95-99 35.7929 37.0 37.0 37.0 37.0 37.0 100-104 35.7753 37.0 37.0 37.0 37.0 37.0 105-109 35.694100000000006 37.0 37.0 37.0 37.0 37.0 110-114 35.680600000000005 37.0 37.0 37.0 37.0 37.0 115-119 35.6595 37.0 37.0 37.0 37.0 37.0 120-124 35.620400000000004 37.0 37.0 37.0 37.0 37.0 125-129 35.531400000000005 37.0 37.0 37.0 37.0 37.0 130-134 35.3995 37.0 37.0 37.0 34.6 37.0 135-139 35.238800000000005 37.0 37.0 37.0 32.2 37.0 140-144 35.039300000000004 37.0 37.0 37.0 27.4 37.0 145-149 34.919650000000004 37.0 37.0 37.0 25.0 37.0 150-151 34.5595 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 1.0 13 1.0 14 5.0 15 5.0 16 1.0 17 1.0 18 3.0 19 3.0 20 0.0 21 0.0 22 2.0 23 5.0 24 3.0 25 6.0 26 9.0 27 9.0 28 12.0 29 15.0 30 25.0 31 39.0 32 67.0 33 116.0 34 213.0 35 591.0 36 2636.0 37 230.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.17310587054692 21.675865529352734 13.723030607124937 29.427997992975413 2 28.4 25.825 29.875 15.9 3 21.55 27.125 31.674999999999997 19.650000000000002 4 23.35 32.05 23.375 21.224999999999998 5 25.874999999999996 37.325 21.175 15.625 6 21.525 38.75 22.525000000000002 17.2 7 19.625 22.3 40.35 17.724999999999998 8 22.400000000000002 27.150000000000002 26.424999999999997 24.025 9 22.525000000000002 24.474999999999998 29.225 23.775 10-14 24.295 29.544999999999998 26.1 20.06 15-19 22.814999999999998 27.3 28.16 21.725 20-24 23.040368165674554 28.772947826521932 27.237256765544494 20.949427242259016 25-29 23.911955977988995 27.213606803401703 28.339169584792394 20.535267633816908 30-34 22.821410705352676 28.61430715357679 27.538769384692348 21.02551275637819 35-39 24.037018509254626 28.0040020010005 28.059029514757377 19.899949974987493 40-44 23.171585792896447 28.3791895947974 28.249124562281143 20.200100050025014 45-49 23.446723361680842 27.48874437218609 28.099049524762382 20.965482741370685 50-54 23.506753376688344 28.019009504752372 27.753876938469237 20.720360180090044 55-59 23.92696348174087 28.16408204102051 27.133566783391693 20.775387693846923 60-64 23.051525762881443 27.57878939469735 28.60430215107554 20.765382691345675 65-69 23.712113634090226 26.43292987896369 28.693608082424728 21.16134840452136 70-74 22.95647823911956 27.383691845922964 28.43421710855428 21.225612806403202 75-79 23.47673836918459 26.638319159579787 28.74437218609305 21.14057028514257 80-84 23.751875937968983 28.429214607303656 26.88844422211106 20.930465232616307 85-89 24.72736368184092 27.478739369684842 26.708354177088545 21.085542771385693 90-94 24.98249124562281 27.668834417208604 27.243621810905456 20.10505252626313 95-99 23.92696348174087 28.729364682341167 26.933466733366686 20.410205102551277 100-104 25.13256628314157 28.50425212606303 26.83341670835418 19.52976488244122 105-109 24.59729864932466 27.688844422211105 26.88844422211106 20.825412706353177 110-114 24.302151075537772 28.66933466733367 27.01350675337669 20.015007503751875 115-119 25.217608804402204 28.339169584792394 26.45822911455728 19.984992496248125 120-124 25.282641320660332 29.119559779889947 26.013006503251624 19.5847923961981 125-129 25.082541270635318 28.07403701850926 26.68334167083542 20.16008004002001 130-134 25.622936055238664 28.09466626638647 26.533573501451013 19.748824176923847 135-139 27.0162097258355 27.051230738443067 26.716029617770666 19.21652991795077 140-144 27.153576788394197 27.478739369684842 26.423211605802898 18.94447223611806 145-149 28.11046075341438 26.68467657211466 26.414527990394713 18.790334684076242 150-151 28.791291291291294 26.2012012012012 26.926926926926924 18.08058058058058 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.0 7 0.0 8 0.5 9 1.5 10 1.0 11 0.5 12 1.0 13 0.5 14 0.0 15 0.5 16 0.5 17 0.0 18 0.5 19 0.5 20 0.0 21 0.0 22 0.0 23 1.0 24 2.0 25 2.0 26 5.5 27 8.5 28 4.5 29 5.0 30 18.5 31 19.5 32 17.5 33 36.5 34 55.5 35 72.5 36 84.0 37 99.5 38 137.5 39 184.0 40 209.5 41 207.5 42 213.5 43 243.0 44 268.5 45 277.0 46 273.5 47 251.0 48 233.5 49 215.0 50 166.5 51 124.0 52 112.0 53 98.5 54 81.5 55 64.5 56 50.0 57 34.0 58 19.0 59 20.0 60 19.0 61 10.5 62 5.0 63 7.0 64 7.5 65 6.5 66 3.5 67 1.5 68 1.5 69 0.5 70 1.0 71 1.0 72 0.0 73 0.0 74 0.0 75 0.0 76 1.0 77 1.0 78 1.0 79 1.0 80 0.0 81 1.0 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.5 91 0.5 92 1.0 93 1.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35000000000000003 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.045 25-29 0.05 30-34 0.05 35-39 0.05 40-44 0.05 45-49 0.05 50-54 0.05 55-59 0.05 60-64 0.05 65-69 0.03 70-74 0.05 75-79 0.05 80-84 0.05 85-89 0.05 90-94 0.05 95-99 0.05 100-104 0.05 105-109 0.05 110-114 0.05 115-119 0.05 120-124 0.05 125-129 0.05 130-134 0.06999999999999999 135-139 0.06 140-144 0.05 145-149 0.055 150-151 0.1 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.05 #Duplication Level Percentage of deduplicated Percentage of total 1 69.22785768357305 45.725 2 18.69795609386828 24.7 3 7.267221801665405 14.399999999999999 4 2.838758516275549 7.5 5 0.984102952308857 3.25 6 0.6056018168054504 2.4 7 0.11355034065102196 0.525 8 0.18925056775170326 1.0 9 0.03785011355034065 0.22499999999999998 >10 0.03785011355034065 0.27499999999999997 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCACCTGCGACAACTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAG 11 0.27499999999999997 No Hit TGGTACTGGATCTGGTCTTGGGTCCCTTCTCTTGGAGCGTTTGTCTGTTG 9 0.22499999999999998 No Hit CTCAAGTGATGAAAGATGCCATGACTGCCCTCAATCAAGAAGTCATGCAA 8 0.2 No Hit ATTAATTTTGATCATGTTGGCTCTACCCTTGTATTAAGGCATGTTAAAAG 8 0.2 No Hit TTGGGCAGCTGTTTATTGCTGGTTTCGGGTCCTCCATGCTGTTTGGGACA 8 0.2 No Hit GTTTCATTGAGCCAAAATGGTTAGCCTACGGTGAGATCATTAACGGACGA 8 0.2 No Hit TATACAAAAAAAGATCTATTTCCAGGATTTGGAAGGCCCTTTGTATTCTA 8 0.2 No Hit CGTCAAAATTTGGACCAGCCTATGTTTCGGGTCCTATTTTCTTCGTTTTC 7 0.17500000000000002 No Hit AGGGAGCACTGCATAGCTTATAAGCTTGTAAGAGATGGCTTCCTCCTCTA 7 0.17500000000000002 No Hit CCGAAATCCCTGATGGTGGTCTCCTTTGTGATCTGCTTTGGTCTGATCCT 7 0.17500000000000002 No Hit CAAGTATTCCATGGATCTGTATGCATCTTACATTGTTGAAGGTCTGAGGC 6 0.15 No Hit CGAACTTGTTTTGTTCGTAGCATGAGTTTTTGAGTTTTATTGGTTGTTCT 6 0.15 No Hit CTCAAGGCATTGGACAAGGAACTTTCAAATGATTTTGAGAGGGTGCTGCT 6 0.15 No Hit GTTAAGGATGAGAAGACCCTTCTCTTTGGTGAGAAGGCTGTCGCTGTTTT 6 0.15 No Hit CTTTAAAACACCCCTTTCACCCTTTCAACTCTTTCGCTTTCTTTTCATCT 6 0.15 No Hit GACAGTATCTCCAGTAATGCTAGCTGATGTTATTTGTGCAACAATGTCCT 6 0.15 No Hit GACGCACAAGGCATTTGCTGAGTACAGAGTAAAATGCCAACTACCTGAAG 6 0.15 No Hit TGCACACCCTCCTCCGCACCGACCTCCGTTTCGGCGTCATCTTCTCCGAC 6 0.15 No Hit GCTGTACTGATCCAAATGCAGCAAACTTTGATCCAACTGCAAGGAGTGAT 6 0.15 No Hit CCTTGTAACACCAATGCATTAATTTGTTTGAAAAATGCTCCAAACATACC 6 0.15 No Hit CCAAGACTTACCCTCAGCAAGCTGGTACCATCCGTAAGAATGGTTACATA 6 0.15 No Hit GAGCAATATGAGAAGGATCCATCAAAAACTGGAAGAGATTCTCAAGATGC 6 0.15 No Hit ATTACGTGAATAATCTCTGTGACCGATTGCCCAGTCCAAATGGAGAATCA 6 0.15 No Hit GACTACACTAAGGATGCTGAACCAACATTGTTAATTACTGCAGATCAAGT 6 0.15 No Hit CAGAGAACACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 6 0.15 No Hit CAGTACCCAAGTGTTCACAAGGCCTTCAACAACAAGGTAACTCAAGAAGA 6 0.15 No Hit CTAAAAACAAATCAGTTGCTGCTGTTGGAAAAGCTGGAGGAGATAAATGG 5 0.125 No Hit CGTGAAAACCCTTTCTCTCTCGAAGCCTCTCCCCCTCCTCTGTCCATCTA 5 0.125 No Hit TCTTGTCAATTATCACAAAAAATATTTTCCAACTTTGTCCAGTATTTTCT 5 0.125 No Hit AGAGAACACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 5 0.125 No Hit CTTCTGCAACCATGGTACAAGTGTTGAATGTCGCATTGATATGAAGAACT 5 0.125 No Hit GCACATAACACCAGTAGAGGCAGGACTGAATTGGGCGATAGGGAAGAGAA 5 0.125 No Hit GCTTGAAGTCATCCATTCCAGGTGGGCCATGCTTGGAGCTCTTGGATGCG 5 0.125 No Hit AGAAAAACGAGAGATTGTGGAAAAAAAAGAAAAAAAAAGAGAGAAGCAGA 5 0.125 No Hit GCTAAGCTTGTATAATTGTTGGTTTCTTTGTCATTTTGTTTAACGCTTGT 5 0.125 No Hit CTTTGTAGCAGTGCTCAGTCCTGACTCAAGCTTCTTCCAAATTGAAGAAA 5 0.125 No Hit CCTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTAC 5 0.125 No Hit AAATTCTCCTGTGGCAAATGAGTGGTCTGAAAGGAGGAGGTTTGAAATTG 5 0.125 No Hit AGGAGCAAGACCAACCCAGTCACCATTCGTGTCCTCAAGGAAAAGCTCTT 5 0.125 No Hit AGTGAGCGTTCTGTTCAATAGTTCAGGAAAGTCCCCTAAATCTTCAGCTC 5 0.125 No Hit GTGCTCACTTCTCAAGTCCTTCCAGCTATCAAGAATTCACCCAAATATGG 5 0.125 No Hit CTGAGGAATTCCTCTCGAAGGTGGATTACTTGCTTGGAAAACAATCCTCC 5 0.125 No Hit AGAAAGTAGAATCTCAGTTTTGTAATAACACATTTTGCAAGCAATGGCAG 5 0.125 No Hit TGTGTTTTCATACTCATCATGAAAATTCGAGGAGGGCTTTATTTCTCCAT 5 0.125 No Hit GAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCA 5 0.125 No Hit GTCCTCTATTTGGATTTACAAAATCCAACGAGCTTTTCGTGGGGAGATTG 5 0.125 No Hit AAAGCTTTCTATGCAAGCAAATAAGCTCATGCCTTTGGAAGATATCACTG 5 0.125 No Hit TGATGAAGAAAGAAGCTACCTTGACAACATCCAATGTACCTGATGTGGTG 5 0.125 No Hit AAGATTTTGGAGAGCAGAGGTGCTAAAGGGAAAATGGACTTTAGGGCATG 5 0.125 No Hit AATGGTCTCAAGTCTGCCGCAGCTTTCCCAGTCAGTACCAGAAAGGCTAA 5 0.125 No Hit CGCAGATGCAAAGGCGCACCACCCAGTCTATCCCAGAGAGCTGAATGATC 5 0.125 No Hit AGCTATTCTTATAGCTACCATTGCCTTCTCTCCCTTATCCATGGCAGCTC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.1125 0.0 0.0 0.0 0.0 78-79 0.16249999999999998 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.23750000000000002 0.0 0.0 0.0 0.0 84-85 0.2875 0.0 0.0 0.0 0.0 86-87 0.4375 0.0 0.0 0.0 0.0 88-89 0.5625 0.0 0.0 0.0 0.0 90-91 0.7 0.0 0.0 0.0 0.0 92-93 1.0375 0.0 0.0 0.0 0.0 94-95 1.2625 0.0 0.0 0.0 0.0 96-97 1.575 0.0 0.0 0.0 0.0 98-99 1.7374999999999998 0.0 0.0 0.0 0.0 100-101 2.0625 0.0 0.0 0.0 0.0 102-103 2.4 0.0 0.0 0.0 0.0 104-105 2.65 0.0 0.0 0.0 0.0 106-107 3.0 0.0 0.0 0.0 0.0 108-109 3.4875 0.0 0.0 0.0 0.0 110-111 3.9375 0.0 0.0 0.0 0.0 112-113 4.35 0.0 0.0 0.0 0.0 114-115 4.85 0.0 0.0 0.0 0.0 116-117 5.5375 0.0 0.0 0.0 0.0 118-119 6.325 0.0 0.0 0.0 0.0 120-121 7.0125 0.0 0.0 0.0 0.0 122-123 7.525 0.0 0.0 0.0 0.0 124-125 8.0375 0.0 0.0 0.0 0.0 126-127 8.7875 0.0 0.0 0.0 0.0 128-129 9.8625 0.0 0.0 0.0 0.0 130-131 10.7375 0.0 0.0 0.0 0.0 132-133 11.7375 0.0 0.0 0.0 0.0 134-135 12.4875 0.0 0.0 0.0 0.0 136-137 13.3 0.0 0.0 0.0 0.0 138-139 14.25 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGCAAT 10 0.006830828 145.0 2 TGCAATT 10 0.006830828 145.0 3 CAATTCT 10 0.006830828 145.0 5 GCAATTC 10 0.006830828 145.0 4 ATTGCAA 10 0.006830828 145.0 1 CGAAATC 10 0.006830828 145.0 2 ATTCTGG 10 0.006830828 145.0 7 GTGGAAG 10 0.006830828 145.0 145 CCGAAAT 10 0.006830828 145.0 1 >>END_MODULE Read 962012 spots for SRR13695449.sra Written 962012 spots for SRR13695449.sra Read 962012 spots for SRR13695449.sra Written 962012 spots for SRR13695449.sra Read 962012 spots for SRR13695449.sra Written 962012 spots for SRR13695449.sra Read 962012 spots for SRR13695449.sra Written 962012 spots for SRR13695449.sra Read 962012 spots for SRR13695449.sra Written 962012 spots for SRR13695449.sra Read 962012 spots for SRR13695449.sra Written 962012 spots for SRR13695449.sra Read 962012 spots for SRR13695449.sra Written 962012 spots for SRR13695449.sra Read 962012 spots for SRR13695449.sra Written 962012 spots for SRR13695449.sra Read 962012 spots for SRR13695449.sra Written 962012 spots for SRR13695449.sra Read 962012 spots for SRR13695449.sra Written 962012 spots for SRR13695449.sra Read 962012 spots for SRR13695449.sra Written 962012 spots for SRR13695449.sra Read 962012 spots for SRR13695449.sra Written 962012 spots for SRR13695449.sra Read 962012 spots for SRR13695449.sra Written 962012 spots for SRR13695449.sra Read 962028 spots for SRR13695449.sra Written 962028 spots for SRR13695449.sra Read 962012 spots for SRR13695449.sra Written 962012 spots for SRR13695449.sra Read 962012 spots for SRR13695449.sra Written 962012 spots for SRR13695449.sra Read 962012 spots for SRR13695449.sra Written 962012 spots for SRR13695449.sra Read 962012 spots for SRR13695449.sra Written 962012 spots for SRR13695449.sra Read 962012 spots for SRR13695449.sra Written 962012 spots for SRR13695449.sra Read 962012 spots for SRR13695449.sra Written 962012 spots for SRR13695449.sra SRR ids: ['SRR13695449.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_gh43xy1p SRR13695449.sra spots: 19240256 blocks: [[1, 962012], [962013, 1924024], [1924025, 2886036], [2886037, 3848048], [3848049, 4810060], [4810061, 5772072], [5772073, 6734084], [6734085, 7696096], [7696097, 8658108], [8658109, 9620120], [9620121, 10582132], [10582133, 11544144], [11544145, 12506156], [12506157, 13468168], [13468169, 14430180], [14430181, 15392192], [15392193, 16354204], [16354205, 17316216], [17316217, 18278228], [18278229, 19240256]] SRR13695449 file size 6516980 SRR13695449 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695449 SRR13695449_1.fastq SRR13695449_2.fastq Input file: SRR13695449_1.fastq Paired file: SRR13695449_2.fastq trimmed: SRR13695449-trimmed-pair1.fastq, SRR13695449-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 03:47:23 2025 >> started Wed Feb 12 03:47:43 2025 >> done (20.194s) 19240256 read pairs processed; of these: 157 ( 0.00%) short read pairs filtered out after trimming by size control 4151 ( 0.02%) empty read pairs filtered out after trimming by size control 19235948 (99.98%) read pairs available; of these: 3434609 (17.86%) trimmed read pairs available after processing 15801339 (82.14%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 3 0.00% 20 0 0.00% 21 1 0.00% 22 3 0.00% 23 5 0.00% 24 0 0.00% 25 2 0.00% 26 4 0.00% 27 1 0.00% 28 7 0.00% 29 13 0.00% 30 7 0.00% 31 5 0.00% 32 12 0.00% 33 10 0.00% 34 9 0.00% 35 17 0.00% 36 12 0.00% 37 23 0.00% 38 22 0.00% 39 27 0.00% 40 20 0.00% 41 30 0.00% 42 29 0.00% 43 40 0.00% 44 45 0.00% 45 41 0.00% 46 50 0.00% 47 82 0.00% 48 86 0.00% 49 84 0.00% 50 114 0.00% 51 158 0.00% 52 176 0.00% 53 173 0.00% 54 208 0.00% 55 236 0.00% 56 277 0.00% 57 283 0.00% 58 447 0.00% 59 506 0.00% 60 551 0.00% 61 676 0.00% 62 771 0.00% 63 807 0.00% 64 882 0.00% 65 1014 0.01% 66 1111 0.01% 67 1341 0.01% 68 1425 0.01% 69 1672 0.01% 70 1895 0.01% 71 2347 0.01% 72 2633 0.01% 73 3008 0.02% 74 3470 0.02% 75 3761 0.02% 76 4145 0.02% 77 4529 0.02% 78 5029 0.03% 79 5793 0.03% 80 6163 0.03% 81 7116 0.04% 82 8052 0.04% 83 9071 0.05% 84 10339 0.05% 85 11039 0.06% 86 12140 0.06% 87 12920 0.07% 88 13797 0.07% 89 15045 0.08% 90 16119 0.08% 91 17286 0.09% 92 18552 0.10% 93 20170 0.10% 94 21528 0.11% 95 23825 0.12% 96 24395 0.13% 97 26110 0.14% 98 26615 0.14% 99 27885 0.14% 100 29489 0.15% 101 29969 0.16% 102 31694 0.16% 103 33643 0.17% 104 34725 0.18% 105 36639 0.19% 106 38425 0.20% 107 39805 0.21% 108 40634 0.21% 109 42336 0.22% 110 42775 0.22% 111 43440 0.23% 112 45384 0.24% 113 46097 0.24% 114 47368 0.25% 115 50002 0.26% 116 51618 0.27% 117 52898 0.27% 118 54551 0.28% 119 55143 0.29% 120 56363 0.29% 121 58013 0.30% 122 58492 0.30% 123 59028 0.31% 124 60652 0.32% 125 60251 0.31% 126 63455 0.33% 127 64969 0.34% 128 66278 0.34% 129 66693 0.35% 130 68149 0.35% 131 67630 0.35% 132 68243 0.35% 133 69626 0.36% 134 69831 0.36% 135 70348 0.37% 136 72942 0.38% 137 73202 0.38% 138 74934 0.39% 139 76996 0.40% 140 77119 0.40% 141 77226 0.40% 142 77388 0.40% 143 78278 0.41% 144 79711 0.41% 145 79845 0.42% 146 80943 0.42% 147 81086 0.42% 148 83451 0.43% 149 84270 0.44% 150 84267 0.44% 151 15801339 82.14% 19235948 reads passed initial QC criterion=sequence-density sequence-density=0.65 sequence-density-rank=1 fanout-score=1.95 fanout-score-rank=30 prefix-density=0.65 prefix-fanout=1.9 sequence=CGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTTCGAGCGAAGAAGGTAATCAATTTCCTTGGCCAATTCCTCCTCTGTGAGATCTGGAAGGTAAGAAAGAGTCTCGAACTTCTTCAATCCAGTTGGTGGCCACACCTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=32 fanout-score=387.17 fanout-score-rank=1 prefix-density=0.18 prefix-fanout=19.8 sequence=CAAAAACAAAGTAGAATGATATTCATCTCCAAAAACCCAATAAAAAAGGAAGAGGTAAAGCATTTTGCCAAGGTCTAAGTACAATTTAAACAAACCACCC criterion=sequence-density sequence-density=0.80 sequence-density-rank=1 fanout-score=2.10 fanout-score-rank=32 prefix-density=0.80 prefix-fanout=2.1 sequence=CCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAATCATCATGTCTAGCACCTGCGACACCTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAGTGAACAAACAAAAGTGTCCGTGAGTGTCTTTATCTTTGTGTCTTCTTCATT criterion=fanout-score sequence-density=0.13 sequence-density-rank=30 fanout-score=17.64 fanout-score-rank=1 prefix-density=0.55 prefix-fanout=4.2 sequence=AATGGCAGCCTCAGTTATGGCTTCACTGAACCTGAAACCATCTCCATTCACGGTTGAGAAGTCTTCAGTGAGAGGCCTCCCAACTCTTTCAAGGAGATCTTTCAAGATT SRR13695449 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 03:48:28 Started mapping on | Feb 12 03:48:29 Finished on | Feb 12 03:50:23 Mapping speed, Million of reads per hour | 607.45 Number of input reads | 19235948 Average input read length | 292 UNIQUE READS: Uniquely mapped reads number | 18142350 Uniquely mapped reads % | 94.31% Average mapped length | 291.18 Number of splices: Total | 17545314 Number of splices: Annotated (sjdb) | 17202769 Number of splices: GT/AG | 17181201 Number of splices: GC/AG | 288889 Number of splices: AT/AC | 9862 Number of splices: Non-canonical | 65362 Mismatch rate per base, % | 0.37% Deletion rate per base | 0.03% Deletion average length | 2.76 Insertion rate per base | 0.02% Insertion average length | 2.32 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 440481 % of reads mapped to multiple loci | 2.29% Number of reads mapped to too many loci | 137672 % of reads mapped to too many loci | 0.72% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.52% % of reads unmapped: other | 0.16% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 653364 653364 653364 N_multimapping 440481 440481 440481 N_noFeature 583440 17582842 903657 N_ambiguous 340461 2110 99765 UnstrandedReadsAssigned:17218449 PositiveStrandReadsAssigned:557398 NegativeStrandReadsAssigned:17138928 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695449 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695449-trimmed-pair1.fastq SRR13695449-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 19,235,948 reads, 17,216,382 reads pseudoaligned [quant] estimated average fragment length: 221.996 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,072 rounds 52401 SRR13695449.ke.tsv 34699 SRR13695449.se.tsv 87100 total ==> SRR13695449.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1797 485 11.961 Potri.005G024800.1.v4.1 1035 814.004 420 22.8663 Potri.004G059700.1.v4.1 961 740.043 5 0.299424 Potri.007G009000.2.v4.1 1416 1195 0 0 Potri.003G141000.2.v4.1 2943 2722 623.408 10.1498 Potri.016G087400.1.v4.1 270 93.7414 1037 490.254 Potri.015G069301.1.v4.1 564 347.535 0 0 Potri.010G195200.1.v4.1 1773 1552 90 2.56994 Potri.012G127500.1.v4.1 977 756.027 87 5.09983 ==> SRR13695449.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 107 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 367 Potri.001G212900.v4.1 3 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 1 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR13695449 completed mapping pipeline successfully