Starting /dee2/code/volunteer_pipeline.sh SRR13695450 current disk space = 3048887975936 free memory = 766598660 SRR13695450 SRAfilesize 753a7f727b58bca83615ddf285b509d5 SRR13695450.sra SRR13695450.sra file validated SRR13695450 is paired end SRR13695450 is conventional basespace SRR13695450 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695450_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.449 37.0 37.0 37.0 37.0 37.0 2 36.148 37.0 37.0 37.0 37.0 37.0 3 36.5835 37.0 37.0 37.0 37.0 37.0 4 36.5075 37.0 37.0 37.0 37.0 37.0 5 36.6 37.0 37.0 37.0 37.0 37.0 6 36.572 37.0 37.0 37.0 37.0 37.0 7 36.415 37.0 37.0 37.0 37.0 37.0 8 36.4955 37.0 37.0 37.0 37.0 37.0 9 36.4985 37.0 37.0 37.0 37.0 37.0 10-14 36.5507 37.0 37.0 37.0 37.0 37.0 15-19 36.47529999999999 37.0 37.0 37.0 37.0 37.0 20-24 36.513600000000004 37.0 37.0 37.0 37.0 37.0 25-29 36.4224 37.0 37.0 37.0 37.0 37.0 30-34 36.4005 37.0 37.0 37.0 37.0 37.0 35-39 36.393 37.0 37.0 37.0 37.0 37.0 40-44 36.33239999999999 37.0 37.0 37.0 37.0 37.0 45-49 36.327200000000005 37.0 37.0 37.0 37.0 37.0 50-54 36.3233 37.0 37.0 37.0 37.0 37.0 55-59 36.3227 37.0 37.0 37.0 37.0 37.0 60-64 36.27380000000001 37.0 37.0 37.0 37.0 37.0 65-69 36.253099999999996 37.0 37.0 37.0 37.0 37.0 70-74 36.254 37.0 37.0 37.0 37.0 37.0 75-79 36.251200000000004 37.0 37.0 37.0 37.0 37.0 80-84 36.1759 37.0 37.0 37.0 37.0 37.0 85-89 36.1972 37.0 37.0 37.0 37.0 37.0 90-94 36.104200000000006 37.0 37.0 37.0 37.0 37.0 95-99 36.1014 37.0 37.0 37.0 37.0 37.0 100-104 36.0184 37.0 37.0 37.0 37.0 37.0 105-109 36.10340000000001 37.0 37.0 37.0 37.0 37.0 110-114 35.9844 37.0 37.0 37.0 37.0 37.0 115-119 35.987199999999994 37.0 37.0 37.0 37.0 37.0 120-124 35.9473 37.0 37.0 37.0 37.0 37.0 125-129 35.8997 37.0 37.0 37.0 37.0 37.0 130-134 35.8745 37.0 37.0 37.0 37.0 37.0 135-139 35.8254 37.0 37.0 37.0 37.0 37.0 140-144 35.794000000000004 37.0 37.0 37.0 37.0 37.0 145-149 35.5966 37.0 37.0 37.0 37.0 37.0 150-151 35.3855 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 1.0 17 1.0 18 0.0 19 0.0 20 0.0 21 0.0 22 2.0 23 3.0 24 0.0 25 2.0 26 3.0 27 7.0 28 15.0 29 22.0 30 19.0 31 42.0 32 64.0 33 97.0 34 128.0 35 347.0 36 2883.0 37 364.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.825000000000003 11.475 9.075 49.625 2 18.834756403817178 15.14314414866901 38.87493721747865 27.14716223003516 3 18.725 18.15 24.349999999999998 38.775 4 21.925 26.85 21.95 29.275000000000002 5 23.549999999999997 31.025000000000002 24.925 20.5 6 20.375 33.45 24.25 21.925 7 14.575 27.925 40.400000000000006 17.1 8 18.55 27.025 30.55 23.875 9 18.925 23.225 35.4 22.45 10-14 19.695 30.514999999999997 27.425 22.365 15-19 19.725 28.475 27.975 23.825 20-24 19.84 28.265 28.349999999999998 23.544999999999998 25-29 20.095 28.904999999999998 27.325 23.674999999999997 30-34 19.655 28.99 27.33 24.025 35-39 20.19 28.075 27.455000000000002 24.279999999999998 40-44 20.215 28.865000000000002 27.91 23.01 45-49 19.215 28.849999999999998 27.42 24.515 50-54 20.19 27.54 28.34 23.93 55-59 19.765 28.34 28.249999999999996 23.645 60-64 19.61 28.084999999999997 28.305000000000003 24.0 65-69 20.365 27.595 28.134999999999998 23.905 70-74 20.369999999999997 28.625 27.595 23.41 75-79 20.305 28.915000000000003 27.800000000000004 22.98 80-84 19.79 28.21 28.544999999999998 23.455000000000002 85-89 21.09 28.799999999999997 27.265 22.845 90-94 20.505000000000003 28.04 27.694999999999997 23.76 95-99 19.814999999999998 28.615000000000002 28.175 23.395 100-104 19.509999999999998 29.054999999999996 27.71 23.724999999999998 105-109 20.369999999999997 27.565 27.744999999999997 24.32 110-114 20.685000000000002 27.779999999999998 27.935 23.599999999999998 115-119 19.650000000000002 28.46 28.244999999999997 23.645 120-124 20.044999999999998 28.389999999999997 27.46 24.104999999999997 125-129 20.005 28.88 27.265 23.849999999999998 130-134 20.78 27.800000000000004 27.400000000000002 24.02 135-139 20.995 28.804999999999996 26.33 23.87 140-144 21.145 27.794999999999998 27.0 24.060000000000002 145-149 20.785 28.29 26.150000000000002 24.775 150-151 21.7 27.712500000000002 26.1125 24.474999999999998 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.0 17 1.0 18 0.0 19 0.0 20 1.0 21 1.5 22 2.0 23 2.0 24 3.0 25 3.0 26 5.0 27 7.0 28 9.0 29 11.5 30 12.5 31 20.0 32 35.0 33 54.5 34 67.5 35 80.5 36 89.0 37 111.5 38 151.5 39 171.0 40 179.0 41 204.5 42 228.0 43 265.0 44 276.5 45 266.5 46 234.0 47 212.5 48 234.0 49 217.0 50 171.5 51 144.0 52 139.5 53 105.0 54 71.5 55 52.0 56 39.0 57 34.5 58 27.0 59 18.5 60 12.0 61 7.5 62 3.0 63 3.0 64 4.0 65 2.5 66 1.5 67 2.0 68 1.0 69 2.0 70 2.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.44999999999999996 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.825 #Duplication Level Percentage of deduplicated Percentage of total 1 69.70143752303723 47.275 2 19.719867305565796 26.75 3 6.597862145226687 13.425 4 2.4327312937707335 6.6000000000000005 5 0.8846295613711759 3.0 6 0.44231478068558794 1.7999999999999998 7 0.11057869517139698 0.525 8 0.07371913011426465 0.4 9 0.036859565057132324 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGGCATTTTGCATTGGTTGTGCAAATAGTCGAGCGCATTAGCCACATCAA 9 0.22499999999999998 No Hit GTCAGCAGATCTTTGGCTTTCTTGCCGATATCGGCGAAGAGTCCAGGTCC 8 0.2 No Hit CACCAGCTCCCTGTCATATACACTGATCTGCGCCAGCGCGTGTTCTAACC 8 0.2 No Hit CCTCCAACAAGTTTCCATGGGCCTCCTCTGCACTCTTCACGGCAGCTTCA 7 0.17500000000000002 No Hit GTCGAGATTGCTGATGGTATCAAAGAGCTTTCCAGTAAGTTCCTTAAGGG 7 0.17500000000000002 No Hit GTTCTCATCATTTTACATATTGACTAAGACAGCAGAAGCTCTCCATGCTT 7 0.17500000000000002 No Hit CCCTACTGGCTTATTGTGGGAGAGTGAATCTGAAAGTTGATATCTCTCAT 6 0.15 No Hit GTCCATTTCTGACCATCCTTGTAAAGAAGGCTATGAACATTATGAGTGGA 6 0.15 No Hit GGCAACTGGAACCTCCATTTGCACTTCTGAGCTGCCTGAGCTTGAACTGT 6 0.15 No Hit CACATAACCATCTCCTGCAAACTGAACACCATTCACAACTGGACATTCAC 6 0.15 No Hit CAGAGTGTTGACTGGAATGGTCAAGTTCCTTCCATCTAAGGCTGTGAGAT 6 0.15 No Hit CCTTCTCCTACCAGTAGTATTTTATGACAACTGCTATAATGCTTCATCCA 6 0.15 No Hit TGTTAACTTTGTTTACGTTCTGACCGCCAGGCCCACCACTTCTTGCAAAA 6 0.15 No Hit TTCCAATTGCCATATGCCCATCAAATGCCTCTTCTCTTGATCTCGCTCCA 6 0.15 No Hit GGCTGAAAGGGCAATTTGGCAACAGCTTTACCTTCAAAGAGCGAATTCAA 6 0.15 No Hit CTAAATTGTAAAATGTGTCCACAAAATCAGGGACCTTTTCAGTTGTTTCG 6 0.15 No Hit GTACGAGCACTGGAAGGAAAACATTATTTCAGTGTTTCTGGAAGCGAATG 6 0.15 No Hit CTATTTTCTATTTCAGCATTCCTTTGGAGAACGAGGAACCAGCGGATCAA 6 0.15 No Hit CCAGGCACTACCCTCTTGTACAAATAGCTATCAAATGTCATCTTTTGCAC 5 0.125 No Hit CCTAATACAAATTTCTCCACGTGGGGTGCTTGAAAGAGCATCATATCCCA 5 0.125 No Hit CTTGTTCTTGATGCACCAACAACATCTGGGGTGCCATGTTTCTTCTTTCT 5 0.125 No Hit GTTCCAAGTCAGATATTGTTGCATCATTCACAGAATAAATTTTATTCCTG 5 0.125 No Hit GTCAAGGATGTAGATGTCATCGGGGCATGCAAACTCCTTCTCACCATCAG 5 0.125 No Hit CCTAAGACTGTGACAGCTAGCTACTTCATAGTTTTGGAGAGAGAACTCAG 5 0.125 No Hit CTCTGAGCTTCGAGCTGTAATCAAAATCTAGAACAGCCAGGTTGATTGAT 5 0.125 No Hit TAGAGACAGAATTAAAAGCAAAGTACTTCGATCAAGATGCTTATAAAGAT 5 0.125 No Hit CTCCTTGTAGACAAACAATTTTTCCAGAAGTGTCATTCCCAGTAGCATAG 5 0.125 No Hit CTTCTGGTCTTGATTTATCCTTGCACTGGTGCTAAGATCTTTGGATGCAC 5 0.125 No Hit CTCAAAAGAAAAAGCACTGTCAATACAGAGTCGGGGAAAATAAGAGACAG 5 0.125 No Hit TATTCTTTCTTCAATTATATTGGTGGTAAAAAAATCTGTTACTCAGTATT 5 0.125 No Hit GCAACTTCGAAAGTCTCTCCTTTGGCATTGAGGAGATCCTCCTCACTCAT 5 0.125 No Hit TCAAAATCCCTTATCTGTGCACGCAAATGCTGAATGTCACCACATGCCTT 5 0.125 No Hit GCTTGCTTCTTCTAATCCACTGGAGAACTTTGCTTATTTTTCTCACATAG 5 0.125 No Hit CACACTTTGTGATTCTCCCATTTTCTACAGACATGGTGCAGGAACTGTCC 5 0.125 No Hit AGCGGCTCTTTCCAAAAGGCGTGAATGCAAATAAAAAACATCTCCTGGAT 5 0.125 No Hit GCCTTGCGCTTAGGCTGAGTAGTACGGCAAGTTTCAACGGCACCAAAGGC 5 0.125 No Hit ACTGATCCTTGGGGAGCAAGAGCGCTGCTGTTTGTGGTGGAGATTGATCG 5 0.125 No Hit CTCCGAGTTGACAGGCTTCTCAAGGTCAACAATATTTGGTTGTGATTCTT 5 0.125 No Hit CTCTCTCGATCTCCCCGGCACTAATGACATTTTCATTACCATCCGAGATA 5 0.125 No Hit CACTCGGCAGGTTCGGGATCCAGGACCGTAGTTCTTGGGATGCGAGTTCC 5 0.125 No Hit CCTGGACTTCAACTGTCCCAAGTTTCTTCAGTCCAACTGTGAATGCTCCA 5 0.125 No Hit CGGAGAGGGGAGGGGTTGCTGGCTCTGTAGAAGTGTTGCGTCTTTGCTGT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.15 0.0 0.0 0.0 0.0 76-77 0.175 0.0 0.0 0.0 0.0 78-79 0.1875 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.225 0.0 0.0 0.0 0.0 84-85 0.275 0.0 0.0 0.0 0.0 86-87 0.3625 0.0 0.0 0.0 0.0 88-89 0.5 0.0 0.0 0.0 0.0 90-91 0.575 0.0 0.0 0.0 0.0 92-93 0.6 0.0 0.0 0.0 0.0 94-95 0.6375 0.0 0.0 0.0 0.0 96-97 0.7625 0.0 0.0 0.0 0.0 98-99 0.975 0.0 0.0 0.0 0.0 100-101 1.2875 0.0 0.0 0.0 0.0 102-103 1.5 0.0 0.0 0.0 0.0 104-105 1.8875 0.0 0.0 0.0 0.0 106-107 2.0125 0.0 0.0 0.0 0.0 108-109 2.25 0.0 0.0 0.0 0.0 110-111 2.6500000000000004 0.0 0.0 0.0 0.0 112-113 3.0 0.0 0.0 0.0 0.0 114-115 3.375 0.0 0.0 0.0 0.0 116-117 3.7625 0.0 0.0 0.0 0.0 118-119 4.05 0.0 0.0 0.0 0.0 120-121 4.4125 0.0 0.0 0.0 0.0 122-123 4.762499999999999 0.0 0.0 0.0 0.0 124-125 5.1125 0.0 0.0 0.0 0.0 126-127 5.8125 0.0 0.0 0.0 0.0 128-129 6.325 0.0 0.0 0.0 0.0 130-131 7.025 0.0 0.0 0.0 0.0 132-133 7.5875 0.0 0.0 0.0 0.0 134-135 8.125 0.0 0.0 0.0 0.0 136-137 8.6125 0.0 0.0 0.0 0.0 138-139 9.162500000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTCTAT 10 0.006830828 145.0 5 CCTTGGG 20 3.5877043E-4 108.75 7 CTGATCC 20 3.5877043E-4 108.75 2 CTTGGGG 20 3.5877043E-4 108.75 8 GATCCTT 20 3.5877043E-4 108.75 4 CACTACC 20 3.5877043E-4 108.75 145 ACTGATC 25 8.7132835E-4 87.0 1 TGATCCT 25 8.7132835E-4 87.0 3 TTGGGGA 25 8.7132835E-4 87.0 9 TCCTTGG 30 0.0017973486 72.5 6 ATCCTTG 30 0.0017973486 72.5 5 CCCATGT 20 0.00593511 29.0 60-64 >>END_MODULE SRR13695450 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695450_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.153 37.0 37.0 37.0 37.0 37.0 2 36.205 37.0 37.0 37.0 37.0 37.0 3 36.03 37.0 37.0 37.0 37.0 37.0 4 36.1225 37.0 37.0 37.0 37.0 37.0 5 36.2825 37.0 37.0 37.0 37.0 37.0 6 36.191 37.0 37.0 37.0 37.0 37.0 7 36.192 37.0 37.0 37.0 37.0 37.0 8 36.251 37.0 37.0 37.0 37.0 37.0 9 36.302 37.0 37.0 37.0 37.0 37.0 10-14 36.253099999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.242999999999995 37.0 37.0 37.0 37.0 37.0 20-24 36.2719 37.0 37.0 37.0 37.0 37.0 25-29 36.156499999999994 37.0 37.0 37.0 37.0 37.0 30-34 36.132600000000004 37.0 37.0 37.0 37.0 37.0 35-39 36.132400000000004 37.0 37.0 37.0 37.0 37.0 40-44 36.1027 37.0 37.0 37.0 37.0 37.0 45-49 36.1129 37.0 37.0 37.0 37.0 37.0 50-54 36.030899999999995 37.0 37.0 37.0 37.0 37.0 55-59 35.9792 37.0 37.0 37.0 37.0 37.0 60-64 36.0039 37.0 37.0 37.0 37.0 37.0 65-69 35.936800000000005 37.0 37.0 37.0 37.0 37.0 70-74 35.898199999999996 37.0 37.0 37.0 37.0 37.0 75-79 35.9117 37.0 37.0 37.0 37.0 37.0 80-84 35.9105 37.0 37.0 37.0 37.0 37.0 85-89 35.7976 37.0 37.0 37.0 37.0 37.0 90-94 35.8618 37.0 37.0 37.0 37.0 37.0 95-99 35.7544 37.0 37.0 37.0 37.0 37.0 100-104 35.8516 37.0 37.0 37.0 37.0 37.0 105-109 35.843 37.0 37.0 37.0 37.0 37.0 110-114 35.6508 37.0 37.0 37.0 37.0 37.0 115-119 35.703100000000006 37.0 37.0 37.0 37.0 37.0 120-124 35.6115 37.0 37.0 37.0 37.0 37.0 125-129 35.6783 37.0 37.0 37.0 37.0 37.0 130-134 35.519999999999996 37.0 37.0 37.0 37.0 37.0 135-139 35.5222 37.0 37.0 37.0 37.0 37.0 140-144 35.354400000000005 37.0 37.0 37.0 34.6 37.0 145-149 35.28435 37.0 37.0 37.0 29.8 37.0 150-151 35.053375 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 4.0 18 0.0 19 1.0 20 0.0 21 0.0 22 1.0 23 4.0 24 6.0 25 11.0 26 10.0 27 10.0 28 18.0 29 11.0 30 26.0 31 36.0 32 70.0 33 126.0 34 204.0 35 624.0 36 2644.0 37 194.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 31.04312938816449 19.332998996990973 16.173520561685056 33.45035105315948 2 29.599999999999998 23.25 30.825000000000003 16.325 3 20.8 28.675 29.25 21.275 4 24.15 35.35 23.225 17.275 5 26.474999999999998 34.725 22.775000000000002 16.025 6 21.5 37.075 24.55 16.875 7 20.8 20.849999999999998 39.35 19.0 8 20.575 25.35 30.025000000000002 24.05 9 22.05 23.575 33.0 21.375 10-14 23.630000000000003 29.015 26.02 21.335 15-19 22.7 28.799999999999997 27.575 20.925 20-24 22.105 28.83 27.975 21.09 25-29 23.29 28.235 27.965 20.51 30-34 22.855 27.450000000000003 27.810000000000002 21.884999999999998 35-39 23.235 28.15 27.169999999999998 21.445 40-44 22.13 28.005000000000003 29.104999999999997 20.76 45-49 22.95 27.474999999999998 28.044999999999998 21.529999999999998 50-54 23.745 27.685 27.794999999999998 20.775 55-59 22.63 26.895000000000003 28.395 22.08 60-64 22.84 27.644999999999996 28.12 21.395 65-69 22.3 28.33 27.67 21.7 70-74 23.630000000000003 27.744999999999997 27.415 21.21 75-79 22.8 28.12 28.21 20.87 80-84 22.96 28.744999999999997 26.369999999999997 21.925 85-89 23.32 27.975 28.105000000000004 20.599999999999998 90-94 24.165 27.76 27.365000000000002 20.71 95-99 23.674999999999997 28.73 27.305 20.29 100-104 23.880000000000003 27.694999999999997 28.15 20.275000000000002 105-109 24.055 27.82 27.68 20.445 110-114 23.77 29.265 26.46 20.505000000000003 115-119 24.735 27.72 27.525 20.02 120-124 25.564999999999998 28.49 26.540000000000003 19.405 125-129 24.4 28.255000000000003 27.339999999999996 20.005 130-134 24.479895979195838 27.690538107621528 27.60552110422084 20.224044808961793 135-139 25.357535753575355 28.14281428142814 26.227622762276226 20.27202720272027 140-144 25.480000000000004 28.244999999999997 26.695 19.580000000000002 145-149 25.37626881344067 28.436421821091056 26.45632281614081 19.73098654932747 150-151 26.479049405878673 28.843026891807376 24.490306441525952 20.187617260787995 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.0 16 0.0 17 1.0 18 1.5 19 1.0 20 0.5 21 0.5 22 3.5 23 3.5 24 1.5 25 3.5 26 5.5 27 6.5 28 9.0 29 15.5 30 18.5 31 29.0 32 39.0 33 44.0 34 46.5 35 58.5 36 79.0 37 98.0 38 125.5 39 170.0 40 189.5 41 214.5 42 252.0 43 259.5 44 264.5 45 259.0 46 269.5 47 257.5 48 224.5 49 188.5 50 158.0 51 138.0 52 106.0 53 93.5 54 83.0 55 71.0 56 54.0 57 41.5 58 35.0 59 25.0 60 17.0 61 6.5 62 7.5 63 9.5 64 5.5 65 1.5 66 0.5 67 1.0 68 1.0 69 0.5 70 0.5 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.02 135-139 0.01 140-144 0.0 145-149 0.005 150-151 0.0625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.15 #Duplication Level Percentage of deduplicated Percentage of total 1 70.46955245781365 48.025 2 19.18561995597946 26.150000000000002 3 6.346294937637564 12.975 4 2.3110785033015406 6.3 5 0.9904622157006602 3.375 6 0.4768892149669846 1.95 7 0.07336757153338225 0.35000000000000003 8 0.07336757153338225 0.4 9 0.036683785766691124 0.22499999999999998 >10 0.036683785766691124 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGAGAACACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 10 0.25 No Hit ATTTAGTTGGTGCTGGTAGTTTTGGGTCTGTGTACAAAGGAGTACTTGCA 9 0.22499999999999998 No Hit CCTCTGTCTCTGATCCAGGGACTTCTGGCTCATCTCCGGCAACTTGTGCT 8 0.2 No Hit AGAGAGAGAAGGCAAAGATCCGTGTCTCTCCAGTCTCGTTTTGGCAAGAC 8 0.2 No Hit AGGAGTTTAAGGTTCCTATTTCAATACTAGGAGCTGAGACAGACCAATTG 7 0.17500000000000002 No Hit TGATATCGTTGTTTTCTGCAACAAATCCCTTGCTGACATGGTCACCATCG 7 0.17500000000000002 No Hit CATTGATAGTGCAGAAATTGTTAGGAGGGGATGGAAAAGATACAACTTAA 6 0.15 No Hit GGAATGAACAGCCAAATATTGTTCCAGCTGATCTAGTCTTCATTATTGAT 6 0.15 No Hit TACAAATCTCTCAAATCCTCCATCGACAAAGCCTCTAAAAAACTCGAAAC 6 0.15 No Hit CAACATGATCCTCAAACAAGTTTTCCTCGATAATCTGCTAATACCGCCAG 6 0.15 No Hit AGGTCTTACCTGAAGGATTTTTGGATCGGACGGCTAACATTGGAAAAATA 6 0.15 No Hit AGAACACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATC 6 0.15 No Hit AGGGAATTGGGGCCATCTTCGGAGATCTCGGATAAGAGGGTGTTTTGGGA 6 0.15 No Hit CTCAGTCTTTCACTCAAGATTGATACTTCACACTAGGGGAAATTGCTGAT 6 0.15 No Hit TCTCAGGACATTGAATGGGATGATCATGGTGATTCTAGTCATGGACAAGG 6 0.15 No Hit ACTCTCTCGCTCTCTTCTTCACTGGGGCTCTACTAGCCGGTGGCATCTAC 6 0.15 No Hit TTTAGTGCCATATAGAAATGGATGAATTGGAGAGCCAAGTGAAAATGCTT 6 0.15 No Hit CTTGGAACAATGTTACAGGACATGGCCATTACACAAAATGGAGGATTCAT 6 0.15 No Hit GTTTGGATTGGCAGAAGCAAAGCTTTAGTTGGATAGAACGCAAGCAAATC 6 0.15 No Hit GGCATGGCGACTAAAAGTCGATTCAAATTCTCTCTGACGTAAAAGCTAGA 5 0.125 No Hit GCCTTTCTTATCGTATGCTTTCTCTTAGCTACCATCGTCTTCTCTCCCCT 5 0.125 No Hit TGAAGGCGTGGAGTTTTTCGATCCATCGCTAGACGATTCCTTTTCATCAT 5 0.125 No Hit CTTCTCTCCCCTGTCCACTTGCACTGCTCGAGAATTGGCCGAGCGAGACG 5 0.125 No Hit ATTGATTAGCTTGGAAATTTGAGAATTGAAAAGAGAGCTGGGTTTTTATT 5 0.125 No Hit CGAAGAGCTTACGAACATAATATGGGGTGAGGCTGATGATAGTGATGACC 5 0.125 No Hit TTTTTTTCCAGCTCCTGGTTGCCTTGAAAGTGCGGTATCCTCAACGGATT 5 0.125 No Hit CCGACTCTTTTTTGTTTTTTGCCTCAGCTGACCGAAACCTTCAGCCTTCC 5 0.125 No Hit GCTGGATCTCTGGAGGACATGCCTGGCAAAAACTTCAATGGACACACCGT 5 0.125 No Hit GAGCAGATCAAGCAAAGCTTAAACACTAATTAATCATGGCAACCAGCTCA 5 0.125 No Hit ATGATGACTGCTACTGCTCTCCCACAATTCAGTGGCCTGAGACCCTCAGC 5 0.125 No Hit CTACATACAAGGTGTTGGATGTGTTGCAGAAAGTTTTCTATAGATCAAAC 5 0.125 No Hit GAAGGTTGCCGTGGAGGTGTCTTTCAAGTCCTTCAACCCACAGCTGTTTG 5 0.125 No Hit CTGGAATGAGGAACTACTATGGTGACAAACAGGGCCAACAATTTCGGGTA 5 0.125 No Hit GTTCAAACAGGTTGTTGAATGTGCCTAGCAACACCACTCGTGTGGCCGTG 5 0.125 No Hit CCCATCTGTGATATTTTGGCCTCTTGAAAGCTTTGATAATAAATGAAAGC 5 0.125 No Hit TGGATTTCTCTGATGCTACATCAAGTGATCGGTCCCTTTTTACAAGCTCT 5 0.125 No Hit CGGATTCCCCGGCTACACTACAATATCTCGCTCCTTATACAGGAGCAGCT 5 0.125 No Hit TTTAATTTTTTCTTCTATTGGTAACGTTAGTAGGTGATCACTGCTTCAAC 5 0.125 No Hit TCCAGAGCAGAGAAAGAATGGCATCTACCTGCAGCCTGCAACAACATGGG 5 0.125 No Hit GCAACTCAACCCGACACTGATCACTCACCTGGAGGAGCAACCATATTCTG 5 0.125 No Hit CAGCGAGTTCTCCATATCCCCTGGTGAAAAGATCGTCTTCAAGAACAATG 5 0.125 No Hit ATTTGATTGTCGGATCATGCCTACAGACTCCTGAAGACGTTGACAAGCTT 5 0.125 No Hit TGTTGAGGTCAATGATAGTGAGAGATTCTTCATAGATTTTGATCAAATGG 5 0.125 No Hit TCCATTTCCAGGCCGCTGATCCCAACAAGCTGAATTTTCCGGTGGCATGG 5 0.125 No Hit CTCACAAGAGTACAAGAGGATGATGCCTGGTAGAATTATTGGTGTTAGTG 5 0.125 No Hit GGAGCCAAAGCATGACTCACAGTGGTTTAAGGTCACTGAACATGGTGGAC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.125 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.15 0.0 0.0 0.0 0.0 76-77 0.175 0.0 0.0 0.0 0.0 78-79 0.1875 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.225 0.0 0.0 0.0 0.0 84-85 0.275 0.0 0.0 0.0 0.0 86-87 0.3625 0.0 0.0 0.0 0.0 88-89 0.5 0.0 0.0 0.0 0.0 90-91 0.575 0.0 0.0 0.0 0.0 92-93 0.6 0.0 0.0 0.0 0.0 94-95 0.6375 0.0 0.0 0.0 0.0 96-97 0.7625 0.0 0.0 0.0 0.0 98-99 0.975 0.0 0.0 0.0 0.0 100-101 1.2875 0.0 0.0 0.0 0.0 102-103 1.5 0.0 0.0 0.0 0.0 104-105 1.8875 0.0 0.0 0.0 0.0 106-107 2.0125 0.0 0.0 0.0 0.0 108-109 2.25 0.0 0.0 0.0 0.0 110-111 2.6625 0.0 0.0 0.0 0.0 112-113 3.0250000000000004 0.0 0.0 0.0 0.0 114-115 3.4 0.0 0.0 0.0 0.0 116-117 3.7875 0.0 0.0 0.0 0.0 118-119 4.0625 0.0 0.0 0.0 0.0 120-121 4.4375 0.0 0.0 0.0 0.0 122-123 4.7875 0.0 0.0 0.0 0.0 124-125 5.1625 0.0 0.0 0.0 0.0 126-127 5.862500000000001 0.0 0.0 0.0 0.0 128-129 6.4125 0.0 0.0 0.0 0.0 130-131 7.125 0.0 0.0 0.0 0.0 132-133 7.6875 0.0 0.0 0.0 0.0 134-135 8.225000000000001 0.0 0.0 0.0 0.0 136-137 8.7375 0.0 0.0 0.0 0.0 138-139 9.287500000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAGAGC 15 1.1411342E-4 145.0 2 TCCAGAG 20 3.5877043E-4 108.75 1 GAGCAGA 20 3.5877043E-4 108.75 5 AGAGCAG 20 3.5877043E-4 108.75 4 GCAGAGA 20 3.5877043E-4 108.75 7 CAGAGAA 25 8.7132835E-4 87.0 8 GTTACCA 25 8.7132835E-4 87.0 145 CAGAGCA 30 0.0017973486 72.5 3 AGAGAAA 30 0.0017973486 72.5 9 AGCAGAG 35 0.0033124194 62.14286 6 AGAAAGA 30 0.0014437955 24.166668 10-14 >>END_MODULE Read 811475 spots for SRR13695450.sra Written 811475 spots for SRR13695450.sra Read 811475 spots for SRR13695450.sra Written 811475 spots for SRR13695450.sra Read 811475 spots for SRR13695450.sra Written 811475 spots for SRR13695450.sra Read 811475 spots for SRR13695450.sra Written 811475 spots for SRR13695450.sra Read 811475 spots for SRR13695450.sra Written 811475 spots for SRR13695450.sra Read 811475 spots for SRR13695450.sra Written 811475 spots for SRR13695450.sra Read 811475 spots for SRR13695450.sra Written 811475 spots for SRR13695450.sra Read 811475 spots for SRR13695450.sra Written 811475 spots for SRR13695450.sra Read 811475 spots for SRR13695450.sra Written 811475 spots for SRR13695450.sra Read 811475 spots for SRR13695450.sra Written 811475 spots for SRR13695450.sra Read 811475 spots for SRR13695450.sra Written 811475 spots for SRR13695450.sra Read 811475 spots for SRR13695450.sra Written 811475 spots for SRR13695450.sra Read 811492 spots for SRR13695450.sra Written 811492 spots for SRR13695450.sra Read 811475 spots for SRR13695450.sra Written 811475 spots for SRR13695450.sra Read 811475 spots for SRR13695450.sra Written 811475 spots for SRR13695450.sra Read 811475 spots for SRR13695450.sra Written 811475 spots for SRR13695450.sra Read 811475 spots for SRR13695450.sra Written 811475 spots for SRR13695450.sra Read 811475 spots for SRR13695450.sra Written 811475 spots for SRR13695450.sra Read 811475 spots for SRR13695450.sra Written 811475 spots for SRR13695450.sra Read 811475 spots for SRR13695450.sra Written 811475 spots for SRR13695450.sra SRR ids: ['SRR13695450.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_lts6q_nt SRR13695450.sra spots: 16229517 blocks: [[1, 811475], [811476, 1622950], [1622951, 2434425], [2434426, 3245900], [3245901, 4057375], [4057376, 4868850], [4868851, 5680325], [5680326, 6491800], [6491801, 7303275], [7303276, 8114750], [8114751, 8926225], [8926226, 9737700], [9737701, 10549175], [10549176, 11360650], [11360651, 12172125], [12172126, 12983600], [12983601, 13795075], [13795076, 14606550], [14606551, 15418025], [15418026, 16229517]] SRR13695450 file size 5493799 SRR13695450 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695450 SRR13695450_1.fastq SRR13695450_2.fastq Input file: SRR13695450_1.fastq Paired file: SRR13695450_2.fastq trimmed: SRR13695450-trimmed-pair1.fastq, SRR13695450-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 03:32:55 2025 >> started Wed Feb 12 03:33:14 2025 >> done (19.109s) 16229517 read pairs processed; of these: 114 ( 0.00%) short read pairs filtered out after trimming by size control 8569 ( 0.05%) empty read pairs filtered out after trimming by size control 16220834 (99.95%) read pairs available; of these: 2221239 (13.69%) trimmed read pairs available after processing 13999595 (86.31%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 3 0.00% 20 2 0.00% 21 1 0.00% 22 1 0.00% 23 2 0.00% 24 5 0.00% 25 4 0.00% 26 3 0.00% 27 2 0.00% 28 7 0.00% 29 5 0.00% 30 4 0.00% 31 5 0.00% 32 2 0.00% 33 6 0.00% 34 8 0.00% 35 5 0.00% 36 9 0.00% 37 16 0.00% 38 10 0.00% 39 17 0.00% 40 27 0.00% 41 29 0.00% 42 35 0.00% 43 20 0.00% 44 32 0.00% 45 20 0.00% 46 31 0.00% 47 50 0.00% 48 70 0.00% 49 85 0.00% 50 60 0.00% 51 81 0.00% 52 120 0.00% 53 116 0.00% 54 116 0.00% 55 143 0.00% 56 140 0.00% 57 211 0.00% 58 226 0.00% 59 227 0.00% 60 261 0.00% 61 333 0.00% 62 383 0.00% 63 426 0.00% 64 529 0.00% 65 565 0.00% 66 667 0.00% 67 798 0.00% 68 896 0.01% 69 880 0.01% 70 1196 0.01% 71 1324 0.01% 72 1515 0.01% 73 1859 0.01% 74 1899 0.01% 75 2175 0.01% 76 2339 0.01% 77 2614 0.02% 78 2979 0.02% 79 3300 0.02% 80 3753 0.02% 81 4188 0.03% 82 4733 0.03% 83 5347 0.03% 84 5826 0.04% 85 6252 0.04% 86 7039 0.04% 87 7343 0.05% 88 8043 0.05% 89 8255 0.05% 90 9021 0.06% 91 9980 0.06% 92 10369 0.06% 93 11655 0.07% 94 12621 0.08% 95 13368 0.08% 96 14210 0.09% 97 14949 0.09% 98 15799 0.10% 99 16158 0.10% 100 17154 0.11% 101 17638 0.11% 102 18784 0.12% 103 19621 0.12% 104 21113 0.13% 105 21747 0.13% 106 23166 0.14% 107 23608 0.15% 108 24540 0.15% 109 25456 0.16% 110 25818 0.16% 111 26275 0.16% 112 27688 0.17% 113 28261 0.17% 114 29449 0.18% 115 31667 0.20% 116 32556 0.20% 117 33106 0.20% 118 34171 0.21% 119 34370 0.21% 120 35354 0.22% 121 36351 0.22% 122 37573 0.23% 123 37750 0.23% 124 39038 0.24% 125 40626 0.25% 126 41646 0.26% 127 42704 0.26% 128 42946 0.26% 129 43281 0.27% 130 44683 0.28% 131 44667 0.28% 132 45374 0.28% 133 46289 0.29% 134 47382 0.29% 135 48462 0.30% 136 49305 0.30% 137 49809 0.31% 138 50901 0.31% 139 51765 0.32% 140 52243 0.32% 141 52171 0.32% 142 53318 0.33% 143 54013 0.33% 144 54823 0.34% 145 55220 0.34% 146 55728 0.34% 147 57215 0.35% 148 57960 0.36% 149 59182 0.36% 150 59468 0.37% 151 13999595 86.31% 16220834 reads passed initial QC criterion=sequence-density sequence-density=0.41 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=25 prefix-density=0.41 prefix-fanout=2.0 sequence=GTGTTGTCGAATCC criterion=fanout-score sequence-density=0.02 sequence-density-rank=28 fanout-score=222.13 fanout-score-rank=1 prefix-density=0.26 prefix-fanout=14.8 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGGGTATGAATGTGTTCTCTGTG criterion=sequence-density sequence-density=0.48 sequence-density-rank=1 fanout-score=2.20 fanout-score-rank=28 prefix-density=0.49 prefix-fanout=2.1 sequence=TGTAAGAGATGGCTTCCTC criterion=fanout-score sequence-density=0.11 sequence-density-rank=24 fanout-score=37.56 fanout-score-rank=1 prefix-density=0.32 prefix-fanout=12.6 sequence=AAAGAAAAGAAAA SRR13695450 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 03:33:57 Started mapping on | Feb 12 03:33:57 Finished on | Feb 12 03:36:06 Mapping speed, Million of reads per hour | 452.67 Number of input reads | 16220834 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 14935051 Uniquely mapped reads % | 92.07% Average mapped length | 293.63 Number of splices: Total | 14533339 Number of splices: Annotated (sjdb) | 14210909 Number of splices: GT/AG | 14229070 Number of splices: GC/AG | 242246 Number of splices: AT/AC | 9107 Number of splices: Non-canonical | 52916 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.03% Deletion average length | 2.82 Insertion rate per base | 0.02% Insertion average length | 2.36 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 381307 % of reads mapped to multiple loci | 2.35% Number of reads mapped to too many loci | 67109 % of reads mapped to too many loci | 0.41% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 5.05% % of reads unmapped: other | 0.11% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 904640 904640 904640 N_multimapping 381307 381307 381307 N_noFeature 605919 14540045 850467 N_ambiguous 243094 1675 91513 UnstrandedReadsAssigned:14086038 PositiveStrandReadsAssigned:393331 NegativeStrandReadsAssigned:13993071 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695450 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695450-trimmed-pair1.fastq SRR13695450-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 16,220,834 reads, 14,055,830 reads pseudoaligned [quant] estimated average fragment length: 241.254 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,085 rounds 52401 SRR13695450.ke.tsv 34699 SRR13695450.se.tsv 87100 total ==> SRR13695450.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1777.75 341 12.4262 Potri.005G024800.1.v4.1 1035 794.746 283 23.0682 Potri.004G059700.1.v4.1 961 720.803 4 0.3595 Potri.007G009000.2.v4.1 1416 1175.75 0 0 Potri.003G141000.2.v4.1 2943 2702.75 829.887 19.8916 Potri.016G087400.1.v4.1 270 88.0753 631 464.12 Potri.015G069301.1.v4.1 564 330.495 0 0 Potri.010G195200.1.v4.1 1773 1532.75 27 1.14117 Potri.012G127500.1.v4.1 977 736.769 75 6.59455 ==> SRR13695450.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 250 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 278 Potri.001G212900.v4.1 1 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 6 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 4 SRR13695450 completed mapping pipeline successfully