Starting /dee2/code/volunteer_pipeline.sh SRR13695451 current disk space = 3049117487104 free memory = 1434020044 SRR13695451 SRAfilesize 7153418fc78636b264ba3b690723136a SRR13695451.sra SRR13695451.sra file validated SRR13695451 is paired end SRR13695451 is conventional basespace SRR13695451 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695451_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5695 37.0 37.0 37.0 37.0 37.0 2 36.3815 37.0 37.0 37.0 37.0 37.0 3 36.573 37.0 37.0 37.0 37.0 37.0 4 36.6245 37.0 37.0 37.0 37.0 37.0 5 36.6015 37.0 37.0 37.0 37.0 37.0 6 36.5395 37.0 37.0 37.0 37.0 37.0 7 36.4885 37.0 37.0 37.0 37.0 37.0 8 36.6105 37.0 37.0 37.0 37.0 37.0 9 36.49 37.0 37.0 37.0 37.0 37.0 10-14 36.5382 37.0 37.0 37.0 37.0 37.0 15-19 36.5581 37.0 37.0 37.0 37.0 37.0 20-24 36.550200000000004 37.0 37.0 37.0 37.0 37.0 25-29 36.4678 37.0 37.0 37.0 37.0 37.0 30-34 36.44510000000001 37.0 37.0 37.0 37.0 37.0 35-39 36.463 37.0 37.0 37.0 37.0 37.0 40-44 36.4331 37.0 37.0 37.0 37.0 37.0 45-49 36.39059999999999 37.0 37.0 37.0 37.0 37.0 50-54 36.349599999999995 37.0 37.0 37.0 37.0 37.0 55-59 36.39 37.0 37.0 37.0 37.0 37.0 60-64 36.3638 37.0 37.0 37.0 37.0 37.0 65-69 36.316 37.0 37.0 37.0 37.0 37.0 70-74 36.312799999999996 37.0 37.0 37.0 37.0 37.0 75-79 36.3041 37.0 37.0 37.0 37.0 37.0 80-84 36.214400000000005 37.0 37.0 37.0 37.0 37.0 85-89 36.3042 37.0 37.0 37.0 37.0 37.0 90-94 36.145799999999994 37.0 37.0 37.0 37.0 37.0 95-99 36.109300000000005 37.0 37.0 37.0 37.0 37.0 100-104 36.122299999999996 37.0 37.0 37.0 37.0 37.0 105-109 36.152499999999996 37.0 37.0 37.0 37.0 37.0 110-114 36.1185 37.0 37.0 37.0 37.0 37.0 115-119 36.0923 37.0 37.0 37.0 37.0 37.0 120-124 35.99399999999999 37.0 37.0 37.0 37.0 37.0 125-129 35.96470000000001 37.0 37.0 37.0 37.0 37.0 130-134 35.973200000000006 37.0 37.0 37.0 37.0 37.0 135-139 35.9127 37.0 37.0 37.0 37.0 37.0 140-144 35.8041 37.0 37.0 37.0 37.0 37.0 145-149 35.606 37.0 37.0 37.0 37.0 37.0 150-151 35.4855 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 4.0 22 0.0 23 0.0 24 1.0 25 2.0 26 6.0 27 8.0 28 9.0 29 15.0 30 15.0 31 35.0 32 67.0 33 75.0 34 125.0 35 328.0 36 2949.0 37 360.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 34.25 10.7 8.774999999999999 46.275 2 17.82302664655606 13.524384112619407 40.246354952237304 28.40623428858723 3 16.775000000000002 16.175 28.749999999999996 38.3 4 22.325 24.625 24.3 28.749999999999996 5 23.5 30.8 25.15 20.549999999999997 6 17.325 36.575 25.025 21.075 7 14.649999999999999 27.474999999999998 40.225 17.65 8 18.15 25.724999999999998 33.2 22.925 9 17.1 23.425 35.675000000000004 23.799999999999997 10-14 19.155 28.294999999999998 28.815 23.735 15-19 20.349999999999998 27.76 28.475 23.415 20-24 19.79 28.884999999999998 27.975 23.35 25-29 18.765 28.62 28.015 24.6 30-34 19.35 28.310000000000002 28.720000000000002 23.62 35-39 20.13 27.62 27.939999999999998 24.310000000000002 40-44 20.195 27.845 28.68 23.28 45-49 19.67 28.449999999999996 27.845 24.035 50-54 20.52 28.92 27.700000000000003 22.86 55-59 19.994999999999997 28.185 27.88 23.94 60-64 19.945 28.24 28.065 23.75 65-69 20.395 27.500000000000004 28.43 23.674999999999997 70-74 19.705000000000002 28.715000000000003 27.595 23.985 75-79 20.549999999999997 28.475 27.665 23.31 80-84 20.125 28.970000000000002 27.700000000000003 23.205000000000002 85-89 20.59 27.97 27.944999999999997 23.494999999999997 90-94 20.44 28.505000000000003 27.725 23.330000000000002 95-99 21.265 27.97 27.529999999999998 23.235 100-104 20.995 28.62 27.425 22.96 105-109 20.48 28.21 27.994999999999997 23.315 110-114 20.485 28.32 28.599999999999998 22.595000000000002 115-119 20.150000000000002 28.515 27.400000000000002 23.935000000000002 120-124 21.060000000000002 28.854999999999997 27.165 22.919999999999998 125-129 20.549999999999997 28.249999999999996 27.37 23.830000000000002 130-134 21.560000000000002 27.485 27.415 23.54 135-139 21.47 28.910000000000004 26.515 23.105 140-144 21.365000000000002 28.32 26.605 23.71 145-149 21.4 27.165 27.560000000000002 23.875 150-151 21.025 27.737499999999997 27.900000000000002 23.3375 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 1.0 22 1.0 23 0.0 24 1.0 25 3.5 26 5.5 27 11.5 28 20.0 29 16.0 30 12.5 31 21.0 32 36.0 33 52.0 34 52.5 35 61.0 36 89.5 37 111.0 38 139.5 39 165.5 40 184.0 41 205.5 42 238.0 43 258.0 44 262.5 45 296.5 46 279.5 47 237.0 48 218.0 49 216.0 50 189.0 51 129.0 52 110.5 53 91.5 54 66.0 55 54.0 56 48.5 57 36.0 58 22.5 59 16.5 60 13.0 61 10.0 62 4.5 63 2.0 64 2.0 65 2.0 66 2.0 67 1.0 68 1.0 69 1.0 70 0.5 71 0.5 72 0.0 73 0.0 74 0.5 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.5499999999999999 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.05 #Duplication Level Percentage of deduplicated Percentage of total 1 67.221801665405 44.4 2 21.49886449659349 28.4 3 7.153671461014382 14.174999999999999 4 2.4224072672218018 6.4 5 0.7948523845571537 2.625 6 0.5299015897047691 2.1 7 0.22710068130204392 1.05 8 0.0757002271006813 0.4 9 0.0757002271006813 0.44999999999999996 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTTTCAGAGAGGAAGATCAAGACCTTTCCCAATATCCACCCCAATCACT 9 0.22499999999999998 No Hit CGCCACGACCACCTCTTTGGTTCTGGTAGTTCCTTCGAGAAACGTATTGT 9 0.22499999999999998 No Hit CTCCCTTCTCCATTCCCTTCAACCCACACATATAGACATAAGTGTTGTCT 8 0.2 No Hit AGAGGAACAAACGTGACATAGAAGAAACAGTACGCATAAGGAAAATCTTG 8 0.2 No Hit CTAAAAGGTAAAATAGAAGATTCAGAACTCTTCGACAGTAAGATCCATAC 7 0.17500000000000002 No Hit GGTCGCTTGGCCCTCCGAAATCCATATTCATCTTCATTTAGCAGGGACCT 7 0.17500000000000002 No Hit GTTGATATGAAACCTGATGCAGCCAATCCACCAAGTCCAAGCCCAACGAT 7 0.17500000000000002 No Hit CCCATGCATTACAGTCAGGATCGGAGGTGGAATTTGTAGGCGTCTTCTCC 7 0.17500000000000002 No Hit CTTGCCATTGTTATTATCTTCACTGTTGTTGTTATCTACAGTGTTGCTAA 7 0.17500000000000002 No Hit GCATCCATTCATCAAGAGTCAGTGACAGACCCATTAATCCATCGATGTCA 7 0.17500000000000002 No Hit CATCAATGTTCATTCTGGGTCCATAAGTGTTGAAAATTCTGGCAATTCGT 6 0.15 No Hit ATCAGGTTTCTGTGATGTATCCTTGAAAGAAGACTCACCTCATTTGAAAA 6 0.15 No Hit CTTCCATCTTGCCATGAGTGTAACAAATCAATCAGGCAGTGTTCCTGCTT 6 0.15 No Hit CCTGGAAGTTGGAAGCTATAGACTTCTCAAGTGCTTCTTGGAAATGTCTG 6 0.15 No Hit CTAAAATAATCCTGGACAACAGAGAGCGTTTGACCCATTGCCCTGTGCAG 6 0.15 No Hit CACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACAC 6 0.15 No Hit CCGTGGCATCTACTCGGACTCCAATAACATCACCGGCCAAATTCTTTGCC 6 0.15 No Hit CTCTTTTTTAAGACAAGTCAACGTCTCATAATGCAATCACACTTGCCAAC 6 0.15 No Hit CCTCACAGTTAATGTTGTCATTAGAGTATCATCTTGAGTAACGTAGGCCG 6 0.15 No Hit GGCAGTAGAACACCAATCTGGAAGATAGTAAGCATCACATATCTTTTTAA 6 0.15 No Hit GGGATTCACAAGTCCTCAAAGAATCAGAACCCCTGGTTGTGGGAACACCA 6 0.15 No Hit GCTAGTCGTCTTGGATAGGAACTAATGACAGCTTGCCTCCATATACATCT 6 0.15 No Hit CTCTCCTGTAGCCTCTTCACAATTTCATATTTGTGTTCTGGAAAAACTCC 6 0.15 No Hit TATCAATGGATCCAGTATGGTTTAAGATCTTTACTCTCGAGCAGTGCATC 6 0.15 No Hit TTGCATGGAACCATTCATCATTAGGTCCTGATTTTCTACTTGCAAGAACT 5 0.125 No Hit GTCTAGTTAAATTACCAAGCGAAGCAGGAATCGGACCGGTGACTGAACTA 5 0.125 No Hit CCGATACAGAGCTCATGCTGGAAGTAGAAGACACTTCCACATCATCATTG 5 0.125 No Hit CTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCT 5 0.125 No Hit GTCTGTCGTAATGCTGAGCCAAGAAAAACAAGGTCCACATAAGTGTTGAA 5 0.125 No Hit CACCCTTCCGTCACGTACATTCTGTTGCCAACCACAGCTGAATCGTACCT 5 0.125 No Hit CTCAGATACTGCTGGACCACCAGGGTGTGCTTTGAGGTACTGTTCCAACT 5 0.125 No Hit CACCTTCAGATGCAAAACTGCATACTCTACTGCTGCCCCAACTCCAGCGT 5 0.125 No Hit CACACGCCTTTTCCTTGAAATATGCCACTCTTTTCCTGGACCACAAGTAC 5 0.125 No Hit CCCAGATCTCTTCCTCCTTGGCTGGAACTGCAGTGATTTTGTTTTTAGGA 5 0.125 No Hit GCTTGAATTCCTGAACCTCCTTAAAATTCATCCATGGCTTCTCCCACACC 5 0.125 No Hit CTTTGATCAACTTTTGAATCACGGAGATGGCGTAAAGAATACCCAGCCCA 5 0.125 No Hit ACCCTGGTTTTGCACCGCAAAGTGATTTAGCTCCTTTTGTTAAACGACGA 5 0.125 No Hit GTTTGATTTTGATGGGTAAATTTTCTAAATCCGTGAATTGGATAACCTGG 5 0.125 No Hit CTCGTATGACCAGCTGTAACATAAACTGACACCACCATATCATTAAACCT 5 0.125 No Hit CTCAAACGAATTGTAAAATCTTGATGTTGATTGAAGGTAAGAATTGGCCA 5 0.125 No Hit CCGTAAGACAATCCTAAAGTACTCTTCCCTAACCACTTAACAAAATAGGT 5 0.125 No Hit CAAAAATGCCATCTTCAACCTTTGGCTTCGGTGGTGGAGCAGCCTTCTTA 5 0.125 No Hit GCGGGGGCGGAGGCGGAGGCGGTGGCTAGGGTTTTGAGGTGTGAAGAGGC 5 0.125 No Hit GCGGAAATCAACTATCGTCAATTGCTTCCAACATTTCATTAGCGAAGTTT 5 0.125 No Hit GGAGGAAGCAGGTGTCTTTGAAGGCAAGAATTTTGGCCCTGCAGGCCGGC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.0625 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.1375 0.0 0.0 0.0 0.0 76-77 0.21250000000000002 0.0 0.0 0.0 0.0 78-79 0.2375 0.0 0.0 0.0 0.0 80-81 0.25 0.0 0.0 0.0 0.0 82-83 0.2875 0.0 0.0 0.0 0.0 84-85 0.3875 0.0 0.0 0.0 0.0 86-87 0.475 0.0 0.0 0.0 0.0 88-89 0.6 0.0 0.0 0.0 0.0 90-91 0.725 0.0 0.0 0.0 0.0 92-93 0.8 0.0 0.0 0.0 0.0 94-95 1.0375 0.0 0.0 0.0 0.0 96-97 1.25 0.0 0.0 0.0 0.0 98-99 1.3625 0.0 0.0 0.0 0.0 100-101 1.7375 0.0 0.0 0.0 0.0 102-103 1.9375 0.0 0.0 0.0 0.0 104-105 2.3125 0.0 0.0 0.0 0.0 106-107 2.625 0.0 0.0 0.0 0.0 108-109 2.875 0.0 0.0 0.0 0.0 110-111 3.1500000000000004 0.0 0.0 0.0 0.0 112-113 3.4875 0.0 0.0 0.0 0.0 114-115 3.9125 0.0 0.0 0.0 0.0 116-117 4.300000000000001 0.0 0.0 0.0 0.0 118-119 4.8625 0.0 0.0 0.0 0.0 120-121 5.4 0.0 0.0 0.0 0.0 122-123 5.75 0.0 0.0 0.0 0.0 124-125 6.1625 0.0 0.0 0.0 0.0 126-127 6.525 0.0 0.0 0.0 0.0 128-129 7.175000000000001 0.0 0.0 0.0 0.0 130-131 7.7875 0.0 0.0 0.0 0.0 132-133 8.375 0.0 0.0 0.0 0.0 134-135 8.8875 0.0 0.0 0.0 0.0 136-137 9.5875 0.0 0.0 0.0 0.0 138-139 10.225 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTCATC 10 0.006830828 145.0 1 TTCATCT 10 0.006830828 145.0 2 CACACTA 10 0.006830828 145.0 8 ATCTTCT 10 0.006830828 145.0 5 ACCATTT 10 0.006830828 145.0 7 CATCTTC 10 0.006830828 145.0 4 ACACTAT 10 0.006830828 145.0 9 TCAAAAC 10 0.006830828 145.0 2 AAAACAC 10 0.006830828 145.0 4 AGTTCCA 10 0.006830828 145.0 145 CAAAACA 10 0.006830828 145.0 3 >>END_MODULE SRR13695451 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695451_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.12025 37.0 37.0 37.0 37.0 37.0 2 36.1355 37.0 37.0 37.0 37.0 37.0 3 36.025 37.0 37.0 37.0 37.0 37.0 4 36.3155 37.0 37.0 37.0 37.0 37.0 5 36.306 37.0 37.0 37.0 37.0 37.0 6 36.182 37.0 37.0 37.0 37.0 37.0 7 36.251 37.0 37.0 37.0 37.0 37.0 8 36.2895 37.0 37.0 37.0 37.0 37.0 9 36.3135 37.0 37.0 37.0 37.0 37.0 10-14 36.274 37.0 37.0 37.0 37.0 37.0 15-19 36.166199999999996 37.0 37.0 37.0 37.0 37.0 20-24 36.17765 37.0 37.0 37.0 37.0 37.0 25-29 36.13655 37.0 37.0 37.0 37.0 37.0 30-34 36.082049999999995 37.0 37.0 37.0 37.0 37.0 35-39 36.10545 37.0 37.0 37.0 37.0 37.0 40-44 36.07385000000001 37.0 37.0 37.0 37.0 37.0 45-49 36.0686 37.0 37.0 37.0 37.0 37.0 50-54 35.98065 37.0 37.0 37.0 37.0 37.0 55-59 36.00855 37.0 37.0 37.0 37.0 37.0 60-64 35.91495 37.0 37.0 37.0 37.0 37.0 65-69 35.891200000000005 37.0 37.0 37.0 37.0 37.0 70-74 35.87695 37.0 37.0 37.0 37.0 37.0 75-79 35.901599999999995 37.0 37.0 37.0 37.0 37.0 80-84 35.877500000000005 37.0 37.0 37.0 37.0 37.0 85-89 35.80935 37.0 37.0 37.0 37.0 37.0 90-94 35.747499999999995 37.0 37.0 37.0 37.0 37.0 95-99 35.72925 37.0 37.0 37.0 37.0 37.0 100-104 35.78175 37.0 37.0 37.0 37.0 37.0 105-109 35.68005 37.0 37.0 37.0 37.0 37.0 110-114 35.65555 37.0 37.0 37.0 37.0 37.0 115-119 35.542449999999995 37.0 37.0 37.0 37.0 37.0 120-124 35.45605 37.0 37.0 37.0 37.0 37.0 125-129 35.4967 37.0 37.0 37.0 37.0 37.0 130-134 35.277750000000005 37.0 37.0 37.0 29.8 37.0 135-139 35.1916 37.0 37.0 37.0 29.8 37.0 140-144 35.074149999999996 37.0 37.0 37.0 25.0 37.0 145-149 34.849599999999995 37.0 37.0 37.0 25.0 37.0 150-151 34.463 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 5.0 14 3.0 15 1.0 16 3.0 17 0.0 18 1.0 19 0.0 20 1.0 21 3.0 22 1.0 23 3.0 24 6.0 25 3.0 26 12.0 27 15.0 28 10.0 29 22.0 30 25.0 31 57.0 32 77.0 33 117.0 34 229.0 35 628.0 36 2581.0 37 197.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 34.797891036906854 20.637710268641726 13.457193070549836 31.107205623901578 2 27.525 24.25 32.1 16.125 3 21.875 26.224999999999998 33.4 18.5 4 25.324999999999996 32.95 23.275000000000002 18.45 5 26.724999999999998 35.675000000000004 22.8 14.799999999999999 6 20.7 39.125 23.200000000000003 16.975 7 20.05 21.775 39.375 18.8 8 21.325 25.275 30.0 23.400000000000002 9 23.25 24.375 29.9 22.475 10-14 22.5 29.98 26.979999999999997 20.54 15-19 23.395 28.455000000000002 27.435 20.715 20-24 23.981199059953 28.266413320666032 27.541377068853446 20.211010550527526 25-29 22.99074768692173 28.097024256064017 28.54713678419605 20.365091272818205 30-34 22.11331699754963 28.624293644046606 28.379256888533277 20.883132469870482 35-39 22.031101555077754 29.411470573528675 27.466373318665934 21.091054552727638 40-44 22.298344751712758 28.63929589438416 27.689153373005954 21.373205980897133 45-49 22.697269726972696 27.817781778177817 27.96279627962796 21.52215221522152 50-54 22.796139806990347 27.876393819690986 28.39641982099105 20.93104655232762 55-59 22.595648912228057 27.81695423855964 28.172043010752688 21.415353838459612 60-64 22.481124056202813 27.881394069703486 29.021451072553628 20.616030801540077 65-69 23.745 28.335 27.435 20.485 70-74 23.350837709427356 28.127031757939484 27.701925481370342 20.820205051262818 75-79 22.684536907381474 28.425685137027408 27.375475095019002 21.514302860572116 80-84 23.54 28.194999999999997 27.1 21.165 85-89 23.055763940985248 28.012003000750184 27.916979244811202 21.015253813453363 90-94 22.58225822582258 28.487848784878487 27.8977897789779 21.032103210321033 95-99 23.36616830841542 28.181409070453523 27.416370818540926 21.03605180259013 100-104 23.43085771442861 29.132283070767688 27.35683920980245 20.080020005001252 105-109 23.795948987246813 27.656914228557138 27.141785446361588 21.405351337834457 110-114 24.24121206060303 27.376368818440923 27.141357067853395 21.241062053102656 115-119 23.730932733183295 28.67716929232308 27.76694173543386 19.824956239059766 120-124 24.71617904476119 29.072268067016754 26.806701675418854 19.4048512128032 125-129 25.08501700340068 27.360472094418885 27.335467093418686 20.219043808761754 130-134 24.7836742860001 26.509278247386586 27.689691391987196 21.01735607462612 135-139 25.14254276282885 28.21346403921176 27.238171451435434 19.40582174652396 140-144 25.631281564078208 27.266363318165908 27.51137556877844 19.59097954897745 145-149 26.44293287986396 27.648294488346504 26.873061918575576 19.035710713213962 150-151 27.201100550275136 28.414207103551774 25.062531265632813 19.32216108054027 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 0.5 13 0.0 14 0.0 15 0.5 16 0.5 17 2.5 18 5.0 19 4.5 20 2.0 21 0.5 22 0.5 23 3.5 24 6.0 25 5.5 26 4.0 27 4.5 28 6.5 29 9.5 30 13.5 31 16.5 32 29.0 33 44.5 34 46.5 35 59.5 36 98.5 37 125.0 38 151.5 39 189.5 40 203.5 41 213.0 42 237.5 43 255.0 44 285.5 45 302.5 46 283.5 47 237.5 48 194.5 49 198.0 50 170.0 51 112.5 52 90.5 53 80.0 54 77.5 55 60.5 56 36.0 57 40.0 58 31.5 59 14.0 60 10.0 61 7.5 62 5.0 63 5.5 64 4.5 65 1.5 66 1.0 67 0.5 68 0.0 69 1.0 70 1.0 71 0.0 72 1.0 73 2.5 74 1.5 75 0.0 76 0.5 77 0.5 78 0.0 79 0.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.42500000000000004 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.005 25-29 0.025 30-34 0.015 35-39 0.005 40-44 0.015 45-49 0.01 50-54 0.005 55-59 0.025 60-64 0.005 65-69 0.0 70-74 0.025 75-79 0.02 80-84 0.0 85-89 0.025 90-94 0.01 95-99 0.005 100-104 0.025 105-109 0.025 110-114 0.005 115-119 0.025 120-124 0.025 125-129 0.02 130-134 0.034999999999999996 135-139 0.03 140-144 0.005 145-149 0.03 150-151 0.05 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.85 #Duplication Level Percentage of deduplicated Percentage of total 1 68.43679880329096 45.75 2 20.680628272251308 27.650000000000002 3 6.806282722513089 13.65 4 2.318623784592371 6.2 5 1.0471204188481675 3.5000000000000004 6 0.4113687359760658 1.6500000000000001 7 0.11219147344801794 0.525 8 0.11219147344801794 0.6 9 0.037397157816005985 0.22499999999999998 >10 0.037397157816005985 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGGGAAAGTCTCTCTGTTCAACCATCGTAGTCAAATTACCAAAACCTCCA 10 0.25 No Hit TGAAACTGGTGATGATCAATCTAGTCCTGCTGAGGAACTTCAGAAGGATG 9 0.22499999999999998 No Hit AGAGAACACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 8 0.2 No Hit AGGAATTTGAGAAAATGAAGGAGAAAGCCCCCGACAACTTCAGGCTGGAC 8 0.2 No Hit GGAAATAAGCACAACAAATGGGGATTCATTTGAAAATGTCAAACAGAGGC 8 0.2 No Hit ATTGGCAATAGATTTACTAAAAGGGAAAAAAAAAACAGAAGACTGGTTTC 7 0.17500000000000002 No Hit GTGATTTTGTTGCTGGTTTTGTTTTAGGAGGTGCTGTATTTGGAACTTTA 7 0.17500000000000002 No Hit GGGATGTTAACATCAATGTGCCTACAGCTCCTGAAGTTGGGTTATACTTG 7 0.17500000000000002 No Hit CCGGGAAGGGGTATAAGGAGTATAAGTTGGGAGATTATTCAAATTGGTTG 6 0.15 No Hit GTTGATTTCGTGATCCGGGTCGATGGCTGAAGTTGAAAAGAATCAGGAAA 6 0.15 No Hit GGACTTGCCAAGCGAGTTGGAGCAAGGATTTTGCTTACATCAACTTCCGA 6 0.15 No Hit AGGCAAGAACTATGATGGTGAAGGCTTGACATCAAAGGGAAACAGAAATG 6 0.15 No Hit CTGAGAGGCAAAGTTTGTTACAGCAAGAATACACTATGGATGATGCATCT 6 0.15 No Hit AGAAAAGGGGCCAAAGACTGTGGTTGATGTTGGGTGTGGGATAGGAGGCA 6 0.15 No Hit AGTTGAGCGTACACGCCTCAAAGTTATAGCAAAAACTTTGCAGCCACCAG 6 0.15 No Hit AAAGAGAGAAAAAAGAAGAAAAAAGTACACAATGGCAGGAATCATGCACA 6 0.15 No Hit GAAAAGAAGAACAAGCTTCAAATTCTGTACCCCTGAGAGATCATGAACCA 6 0.15 No Hit TGGAGGAGTTAAGGGACTGTTTACAGGAGTTGGTCCCCGTGTTGCTCGTG 6 0.15 No Hit CTTCGATTAACAGCTGGTGTCTCACCTCTGTCTCTGCCTCTAAGAGATCA 6 0.15 No Hit AAGCATTTATAAGGACCAAAACCGTGGTGCTTCTTATTCTAAGCTGTTAC 5 0.125 No Hit TGCTTTTGAACTCCAGAAATCTGTTAGAGACCTCCAGCCTTGTAGTTCTT 5 0.125 No Hit ATGAGGATGGTGGGATTATGTGATTATGTGGCGGCATTCCACTCTATCAT 5 0.125 No Hit GAAGATCCCTCTGTCAAGGAAGTGGACGATTTGATGATCCGGAGATTTCT 5 0.125 No Hit GTCGAAAATAAAAAGCAAAGAAGTAGATTGGTTACCAACAGGGAGGGAGG 5 0.125 No Hit CCGGGTTTGCCCGTCCCATGTGCTTGATTTCCAACCAGGGGAAGCTTTTG 5 0.125 No Hit TGATGCCTCAGTAGTGGGGGATTCTGAGGATTGTAGCACACCTAGAGGAA 5 0.125 No Hit GTCAAGTTTATGACAAGCCCCATGGAGAAAGACAAGGAGACTGTAGCAAT 5 0.125 No Hit CGTGAAGCGAGTCATGCAGGATGCAGCAATTGAGAAGTAGAGGGAGCTCA 5 0.125 No Hit ATTCACGAGGCAAACACAGGTTGATTGTGATCTAATTCATGTTCAGCCGG 5 0.125 No Hit ATCAATGGTCCATAGGGTCCCCGATGTTGACCCCGCGATCGTTCTTCGCC 5 0.125 No Hit CCACACAAAAATAACTACCACCACCACAATTATCACCACCGACGTCTACC 5 0.125 No Hit GAACAATACCATCGGGTCTCGGTAGAAAGGTGGTTTTGAACTGCTCTGGA 5 0.125 No Hit CTTACATTTCACCTTCTCAAGGATGTGCCCGGGCTTATTTCAAAAAACGT 5 0.125 No Hit CAGCAACAGAGAAGAGCAAAGAACTTGCAGAGCAGAGATGGCATCCATGA 5 0.125 No Hit TGCACGTTGGGTCAGCAAGGTTTCTTGACAAGAAATGAACTGTCAGCTTT 5 0.125 No Hit AGTAGGGTCACAAAAGCCAACATCTGCTAATAAACCTGATGCAACATCGA 5 0.125 No Hit GCTCTTTTCTTTTATGGTGCCAAGACTTCCCGAGTGAAATCAAGGTATAA 5 0.125 No Hit CAGCTGGTGTCTCACCTCTGTCTCTGCCTCTAAGAGATCACTACCCGCAT 5 0.125 No Hit CTTCTATTCTAGAAGTGAGTTTATTTTTGACCCTGTTAAATGGCCACGGT 5 0.125 No Hit GTCCACTTCCACCTTATTTGTGGCTTCTGACCAATTTGGTAAGGTTCCTC 5 0.125 No Hit GGGCAACAGGGGAAGAAGGGAATTTGGATCAAGTTGCCAATCGCACTGGC 5 0.125 No Hit ATTTGGAACCAAGAAATCGCCTCCTCCTCCTCCACCAAAGAAATCCTCCC 5 0.125 No Hit GTCTCTATAGGCTTAATTGTGAGGTTTGCTGTGCCTAAACCAATAGAAGT 5 0.125 No Hit GTGGGAGGATCAGCTAACAGTCTTGAAATCGAGAGTCTTGCTCGTTTTGC 5 0.125 No Hit ATACCAGCCATAGCTTGGGTGCTTTTCAACATCCTCCAACCAGCACTTAA 5 0.125 No Hit GTTTGATTTTTTTTCAATTTTATCCTTTGATATTGGGCTAATTAAAAATT 5 0.125 No Hit GAAAAACAAAATATACAAGAGAGAACACGATGGCTCAAACCATGGTGCTC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.0625 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.1375 0.0 0.0 0.0 0.0 76-77 0.1875 0.0 0.0 0.0 0.0 78-79 0.21250000000000002 0.0 0.0 0.0 0.0 80-81 0.225 0.0 0.0 0.0 0.0 82-83 0.2625 0.0 0.0 0.0 0.0 84-85 0.35 0.0 0.0 0.0 0.0 86-87 0.42500000000000004 0.0 0.0 0.0 0.0 88-89 0.55 0.0 0.0 0.0 0.0 90-91 0.675 0.0 0.0 0.0 0.0 92-93 0.75 0.0 0.0 0.0 0.0 94-95 0.9875 0.0 0.0 0.0 0.0 96-97 1.2000000000000002 0.0 0.0 0.0 0.0 98-99 1.3125 0.0 0.0 0.0 0.0 100-101 1.6875 0.0 0.0 0.0 0.0 102-103 1.9500000000000002 0.0 0.0 0.0 0.0 104-105 2.3125 0.0 0.0 0.0 0.0 106-107 2.625 0.0 0.0 0.0 0.0 108-109 2.875 0.0 0.0 0.0 0.0 110-111 3.1500000000000004 0.0 0.0 0.0 0.0 112-113 3.4625000000000004 0.0 0.0 0.0 0.0 114-115 3.8875 0.0 0.0 0.0 0.0 116-117 4.275 0.0 0.0 0.0 0.0 118-119 4.8375 0.0 0.0 0.0 0.0 120-121 5.375 0.0 0.0 0.0 0.0 122-123 5.75 0.0 0.0 0.0 0.0 124-125 6.1625 0.0 0.0 0.0 0.0 126-127 6.525 0.0 0.0 0.0 0.0 128-129 7.175000000000001 0.0 0.0 0.0 0.0 130-131 7.7875 0.0 0.0 0.0 0.0 132-133 8.35 0.0 0.0 0.0 0.0 134-135 8.8625 0.0 0.0 0.0 0.0 136-137 9.55 0.0 0.0 0.0 0.0 138-139 10.2 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCAGAAT 10 0.006830828 145.0 1 AGAATTC 10 0.006830828 145.0 3 CTGAACA 10 0.006830828 145.0 145 TTGGGAA 10 0.006830828 145.0 2 TCTTGGT 10 0.006830828 145.0 8 CAGAATT 10 0.006830828 145.0 2 TGGGAAA 10 0.006830828 145.0 3 >>END_MODULE Read 1132507 spots for SRR13695451.sra Written 1132507 spots for SRR13695451.sra Read 1132507 spots for SRR13695451.sra Written 1132507 spots for SRR13695451.sra Read 1132507 spots for SRR13695451.sra Written 1132507 spots for SRR13695451.sra Read 1132507 spots for SRR13695451.sra Written 1132507 spots for SRR13695451.sra Read 1132507 spots for SRR13695451.sra Written 1132507 spots for SRR13695451.sra Read 1132507 spots for SRR13695451.sra Written 1132507 spots for SRR13695451.sra Read 1132507 spots for SRR13695451.sra Written 1132507 spots for SRR13695451.sra Read 1132507 spots for SRR13695451.sra Written 1132507 spots for SRR13695451.sra Read 1132507 spots for SRR13695451.sra Written 1132507 spots for SRR13695451.sra Read 1132507 spots for SRR13695451.sra Written 1132507 spots for SRR13695451.sra Read 1132507 spots for SRR13695451.sra Written 1132507 spots for SRR13695451.sra Read 1132507 spots for SRR13695451.sra Written 1132507 spots for SRR13695451.sra Read 1132507 spots for SRR13695451.sra Written 1132507 spots for SRR13695451.sra Read 1132507 spots for SRR13695451.sra Written 1132507 spots for SRR13695451.sra Read 1132507 spots for SRR13695451.sra Written 1132507 spots for SRR13695451.sra Read 1132507 spots for SRR13695451.sra Written 1132507 spots for SRR13695451.sra Read 1132507 spots for SRR13695451.sra Written 1132507 spots for SRR13695451.sra Read 1132522 spots for SRR13695451.sra Written 1132522 spots for SRR13695451.sra Read 1132507 spots for SRR13695451.sra Written 1132507 spots for SRR13695451.sra Read 1132507 spots for SRR13695451.sra Written 1132507 spots for SRR13695451.sra SRR ids: ['SRR13695451.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_7mk6lla5 SRR13695451.sra spots: 22650155 blocks: [[1, 1132507], [1132508, 2265014], [2265015, 3397521], [3397522, 4530028], [4530029, 5662535], [5662536, 6795042], [6795043, 7927549], [7927550, 9060056], [9060057, 10192563], [10192564, 11325070], [11325071, 12457577], [12457578, 13590084], [13590085, 14722591], [14722592, 15855098], [15855099, 16987605], [16987606, 18120112], [18120113, 19252619], [19252620, 20385126], [20385127, 21517633], [21517634, 22650155]] SRR13695451 file size 7675813 SRR13695451 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695451 SRR13695451_1.fastq SRR13695451_2.fastq Input file: SRR13695451_1.fastq Paired file: SRR13695451_2.fastq trimmed: SRR13695451-trimmed-pair1.fastq, SRR13695451-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 04:14:08 2025 >> started Wed Feb 12 04:14:34 2025 >> done (26.421s) 22650155 read pairs processed; of these: 188 ( 0.00%) short read pairs filtered out after trimming by size control 1986 ( 0.01%) empty read pairs filtered out after trimming by size control 22647981 (99.99%) read pairs available; of these: 3116312 (13.76%) trimmed read pairs available after processing 19531669 (86.24%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 2 0.00% 20 2 0.00% 21 1 0.00% 22 10 0.00% 23 4 0.00% 24 2 0.00% 25 1 0.00% 26 4 0.00% 27 4 0.00% 28 6 0.00% 29 3 0.00% 30 2 0.00% 31 3 0.00% 32 5 0.00% 33 9 0.00% 34 8 0.00% 35 6 0.00% 36 9 0.00% 37 22 0.00% 38 20 0.00% 39 23 0.00% 40 26 0.00% 41 31 0.00% 42 40 0.00% 43 50 0.00% 44 73 0.00% 45 40 0.00% 46 67 0.00% 47 64 0.00% 48 102 0.00% 49 130 0.00% 50 138 0.00% 51 136 0.00% 52 143 0.00% 53 225 0.00% 54 183 0.00% 55 232 0.00% 56 236 0.00% 57 318 0.00% 58 279 0.00% 59 405 0.00% 60 514 0.00% 61 592 0.00% 62 572 0.00% 63 735 0.00% 64 830 0.00% 65 894 0.00% 66 1007 0.00% 67 1140 0.01% 68 1264 0.01% 69 1510 0.01% 70 1718 0.01% 71 2033 0.01% 72 2314 0.01% 73 2632 0.01% 74 2997 0.01% 75 3413 0.02% 76 3615 0.02% 77 4032 0.02% 78 4406 0.02% 79 5050 0.02% 80 5486 0.02% 81 6315 0.03% 82 6893 0.03% 83 7764 0.03% 84 8573 0.04% 85 9186 0.04% 86 10054 0.04% 87 10849 0.05% 88 11868 0.05% 89 12493 0.06% 90 13747 0.06% 91 14423 0.06% 92 15537 0.07% 93 16739 0.07% 94 18027 0.08% 95 19581 0.09% 96 20457 0.09% 97 21688 0.10% 98 21818 0.10% 99 23228 0.10% 100 24512 0.11% 101 25055 0.11% 102 26705 0.12% 103 28299 0.12% 104 29456 0.13% 105 30750 0.14% 106 32588 0.14% 107 33227 0.15% 108 34334 0.15% 109 35707 0.16% 110 37092 0.16% 111 38133 0.17% 112 39767 0.18% 113 40360 0.18% 114 41799 0.18% 115 43598 0.19% 116 45262 0.20% 117 46167 0.20% 118 47583 0.21% 119 48546 0.21% 120 49761 0.22% 121 51023 0.23% 122 51878 0.23% 123 53237 0.24% 124 54495 0.24% 125 55768 0.25% 126 57591 0.25% 127 59225 0.26% 128 60015 0.26% 129 60375 0.27% 130 62091 0.27% 131 62698 0.28% 132 62866 0.28% 133 64800 0.29% 134 65341 0.29% 135 66478 0.29% 136 68321 0.30% 137 68808 0.30% 138 70281 0.31% 139 73125 0.32% 140 73014 0.32% 141 73467 0.32% 142 74407 0.33% 143 74324 0.33% 144 76713 0.34% 145 77153 0.34% 146 78180 0.35% 147 79345 0.35% 148 80466 0.36% 149 80634 0.36% 150 82433 0.36% 151 19531669 86.24% 22647981 reads passed initial QC criterion=sequence-density sequence-density=0.31 sequence-density-rank=1 fanout-score=2.28 fanout-score-rank=28 prefix-density=0.33 prefix-fanout=2.2 sequence=TGAGATGCCTCAGTGCATCCAAACATGGGTAG criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=425.38 fanout-score-rank=1 prefix-density=0.27 prefix-fanout=18.5 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAG criterion=sequence-density sequence-density=0.80 sequence-density-rank=1 fanout-score=2.02 fanout-score-rank=31 prefix-density=0.80 prefix-fanout=2.0 sequence=CTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAGTGAACAAACAAAAGTGTCCGTGAGTGTCTTTATCTTTGTGTCTTCTTCA criterion=fanout-score sequence-density=0.13 sequence-density-rank=23 fanout-score=35.80 fanout-score-rank=1 prefix-density=0.37 prefix-fanout=12.7 sequence=AAAGAAAAGAAAA SRR13695451 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 04:15:15 Started mapping on | Feb 12 04:15:15 Finished on | Feb 12 04:17:27 Mapping speed, Million of reads per hour | 617.67 Number of input reads | 22647981 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 21477093 Uniquely mapped reads % | 94.83% Average mapped length | 293.51 Number of splices: Total | 21117648 Number of splices: Annotated (sjdb) | 20621912 Number of splices: GT/AG | 20678551 Number of splices: GC/AG | 338868 Number of splices: AT/AC | 13581 Number of splices: Non-canonical | 86648 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.03% Deletion average length | 2.85 Insertion rate per base | 0.02% Insertion average length | 2.39 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 517569 % of reads mapped to multiple loci | 2.29% Number of reads mapped to too many loci | 50759 % of reads mapped to too many loci | 0.22% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.58% % of reads unmapped: other | 0.08% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 653617 653617 653617 N_multimapping 517569 517569 517569 N_noFeature 914497 20959702 1251329 N_ambiguous 306386 2156 124300 UnstrandedReadsAssigned:20256210 PositiveStrandReadsAssigned:515235 NegativeStrandReadsAssigned:20101464 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695451 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695451-trimmed-pair1.fastq SRR13695451-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 22,647,981 reads, 20,140,061 reads pseudoaligned [quant] estimated average fragment length: 241.384 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,096 rounds 52401 SRR13695451.ke.tsv 34699 SRR13695451.se.tsv 87100 total ==> SRR13695451.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1777.62 923 24.8487 Potri.005G024800.1.v4.1 1035 794.616 404 24.3312 Potri.004G059700.1.v4.1 961 720.666 0 0 Potri.007G009000.2.v4.1 1416 1175.62 0 0 Potri.003G141000.2.v4.1 2943 2702.62 992 17.5658 Potri.016G087400.1.v4.1 270 89.1021 1297 696.613 Potri.015G069301.1.v4.1 564 332.388 0 0 Potri.010G195200.1.v4.1 1773 1532.62 67 2.09209 Potri.012G127500.1.v4.1 977 736.661 93 6.04165 ==> SRR13695451.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 143 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 302 Potri.001G212900.v4.1 11 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 7 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 6 SRR13695451 completed mapping pipeline successfully