Starting /dee2/code/volunteer_pipeline.sh SRR13695452 current disk space = 3049210609664 free memory = 1432672660 SRR13695452 SRAfilesize e152f9df17f862b8617dd29f00adf01e SRR13695452.sra SRR13695452.sra file validated SRR13695452 is paired end SRR13695452 is conventional basespace SRR13695452 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695452_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5595 37.0 37.0 37.0 37.0 37.0 2 36.33925 37.0 37.0 37.0 37.0 37.0 3 36.622 37.0 37.0 37.0 37.0 37.0 4 36.5995 37.0 37.0 37.0 37.0 37.0 5 36.6715 37.0 37.0 37.0 37.0 37.0 6 36.5845 37.0 37.0 37.0 37.0 37.0 7 36.502 37.0 37.0 37.0 37.0 37.0 8 36.5885 37.0 37.0 37.0 37.0 37.0 9 36.484 37.0 37.0 37.0 37.0 37.0 10-14 36.5818 37.0 37.0 37.0 37.0 37.0 15-19 36.568200000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.4966 37.0 37.0 37.0 37.0 37.0 25-29 36.4264 37.0 37.0 37.0 37.0 37.0 30-34 36.4111 37.0 37.0 37.0 37.0 37.0 35-39 36.4219 37.0 37.0 37.0 37.0 37.0 40-44 36.373900000000006 37.0 37.0 37.0 37.0 37.0 45-49 36.368900000000004 37.0 37.0 37.0 37.0 37.0 50-54 36.3384 37.0 37.0 37.0 37.0 37.0 55-59 36.2764 37.0 37.0 37.0 37.0 37.0 60-64 36.2888 37.0 37.0 37.0 37.0 37.0 65-69 36.272999999999996 37.0 37.0 37.0 37.0 37.0 70-74 36.23479999999999 37.0 37.0 37.0 37.0 37.0 75-79 36.275999999999996 37.0 37.0 37.0 37.0 37.0 80-84 36.1739 37.0 37.0 37.0 37.0 37.0 85-89 36.2269 37.0 37.0 37.0 37.0 37.0 90-94 36.20269999999999 37.0 37.0 37.0 37.0 37.0 95-99 36.0596 37.0 37.0 37.0 37.0 37.0 100-104 36.093999999999994 37.0 37.0 37.0 37.0 37.0 105-109 36.1109 37.0 37.0 37.0 37.0 37.0 110-114 36.111900000000006 37.0 37.0 37.0 37.0 37.0 115-119 36.096 37.0 37.0 37.0 37.0 37.0 120-124 35.9617 37.0 37.0 37.0 37.0 37.0 125-129 35.937400000000004 37.0 37.0 37.0 37.0 37.0 130-134 35.9433 37.0 37.0 37.0 37.0 37.0 135-139 35.857 37.0 37.0 37.0 37.0 37.0 140-144 35.6881 37.0 37.0 37.0 37.0 37.0 145-149 35.5247 37.0 37.0 37.0 37.0 37.0 150-151 35.288 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 3.0 25 0.0 26 4.0 27 13.0 28 12.0 29 23.0 30 30.0 31 38.0 32 53.0 33 78.0 34 116.0 35 346.0 36 2946.0 37 337.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 27.35 10.299999999999999 10.125 52.225 2 18.434913468773516 13.945322297466767 41.20892901931277 26.410835214446955 3 17.175 17.424999999999997 25.724999999999998 39.675 4 21.75 26.174999999999997 21.05 31.025000000000002 5 24.725 32.300000000000004 23.25 19.725 6 19.175 38.475 24.099999999999998 18.25 7 15.35 25.5 42.075 17.075000000000003 8 19.0 25.15 31.574999999999996 24.275 9 16.25 22.75 36.175000000000004 24.825 10-14 18.740000000000002 29.95 27.634999999999998 23.674999999999997 15-19 19.775000000000002 28.43 27.500000000000004 24.295 20-24 20.330000000000002 27.99 28.065 23.615 25-29 19.225 28.499999999999996 28.76 23.515 30-34 19.765 28.439999999999998 27.985 23.810000000000002 35-39 19.814999999999998 28.275 28.915000000000003 22.994999999999997 40-44 19.935 28.994999999999997 27.029999999999998 24.04 45-49 19.89 29.475 26.88 23.755000000000003 50-54 20.165 28.384999999999998 27.595 23.855 55-59 19.695 28.910000000000004 27.91 23.485 60-64 20.225 27.965 27.98 23.830000000000002 65-69 20.915 28.599999999999998 27.02 23.465 70-74 20.36 27.595 27.955000000000002 24.09 75-79 19.915 29.175 27.575 23.335 80-84 20.075000000000003 27.93 28.33 23.665 85-89 21.02 27.46 27.61 23.91 90-94 20.28 28.665000000000003 27.36 23.695 95-99 21.044999999999998 27.744999999999997 28.065 23.145 100-104 20.805 28.12 27.439999999999998 23.635 105-109 21.525 27.99 27.565 22.919999999999998 110-114 20.93 28.535 27.12 23.415 115-119 20.724999999999998 28.634999999999998 27.625 23.015 120-124 21.745 27.450000000000003 26.91 23.895 125-129 20.995 28.804999999999996 26.495 23.705000000000002 130-134 20.73 28.455000000000002 26.99 23.825 135-139 21.44 27.810000000000002 27.08 23.669999999999998 140-144 21.61 28.035 26.845000000000002 23.51 145-149 21.34 28.494999999999997 26.765 23.400000000000002 150-151 21.575 27.212500000000002 26.887499999999996 24.325 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 1.0 19 1.0 20 2.0 21 2.0 22 0.0 23 0.0 24 1.5 25 5.0 26 7.5 27 11.0 28 13.0 29 15.0 30 19.5 31 26.5 32 33.0 33 38.0 34 57.5 35 69.5 36 82.0 37 125.0 38 148.0 39 156.0 40 185.5 41 218.0 42 225.0 43 231.5 44 263.0 45 266.5 46 252.5 47 247.0 48 227.0 49 206.5 50 165.5 51 139.5 52 125.5 53 103.5 54 83.0 55 61.5 56 50.5 57 38.5 58 30.5 59 22.0 60 16.0 61 12.0 62 8.5 63 2.0 64 0.0 65 0.5 66 1.0 67 0.5 68 0.0 69 1.0 70 1.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.325 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.475 #Duplication Level Percentage of deduplicated Percentage of total 1 71.52245345016429 48.975 2 17.99926980649872 24.65 3 6.425702811244979 13.200000000000001 4 2.2635998539612996 6.2 5 1.095290251916758 3.75 6 0.4016064257028112 1.6500000000000001 7 0.10952902519167579 0.525 8 0.10952902519167579 0.6 9 0.07301935012778386 0.44999999999999996 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTGCACTGTGGCAAAAGAAAGCTCTTCCCTGACGTCCTAGAAAACCG 9 0.22499999999999998 No Hit GTTCCAGGAACGAAGTTAGTGGCGTATGCCCAAGCATTGTTGGCCACTGG 9 0.22499999999999998 No Hit CACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACA 8 0.2 No Hit CTGGGATTCTTTCCCTGTCAAAATTAGCAAGTTTCTCCACCAAATGATAG 8 0.2 No Hit GTAAGAATAAGAAATCTAGAAACTAAAGATGGGTTTAAATGGAGACAGTT 8 0.2 No Hit GGGAGAACAAAATTATACATAGGGATTCACAAGGGGGGAGAATTTTTTTG 7 0.17500000000000002 No Hit TGGATGTTAAGTTCTAACAATGCAGAAAAACAATTTTTCCATGGGCTTTC 7 0.17500000000000002 No Hit GCTTGATCTTTGCGGCGGCGACGCTCCCGAGGTCAGCTTTCTCACTGAGA 7 0.17500000000000002 No Hit CTGCAGTGCAATCTTGAGCACTGTTGAAGATGGGATGGCAAACCCTACAC 6 0.15 No Hit CCCAGACACTCAGTGTAGAACTTTATAGTCCTGTCCAAATCCCCAACACG 6 0.15 No Hit GTCCAAGGGATTTGTTCCATGAGAGGAGCCGCAACTTCTCGGAGAATCCA 6 0.15 No Hit ATTAAAGCTTCCCTGTCAGGTTTGAAAACCAGGTTCTTGTATGCAAACCA 6 0.15 No Hit CTCGGAGAAACTCAAGTCTGGGTACATGCTGCATCCATTGCAGCCACTGC 6 0.15 No Hit CTCTGCTTGGAGATTTGACTAGTTTCCTCTTTAAGTTTGGCAGCCTCTTT 6 0.15 No Hit CTCTAATGGAAATCACTCTGCCACTAGCAGACACATCCCTGAAGTGAACT 6 0.15 No Hit GATGAAGGTTCTCCAGCTTTTTCAACCTTGATGACAAGACTTGGAAACCT 6 0.15 No Hit CGAGAACTCTCCAAGGGTCATGGTAATGATTCTCTTGAAGAAGCTCGTGA 6 0.15 No Hit GTTCCTCGATCTCAGCTACTCGTATTCAACCAAGTAATAGCTGGCCACGC 6 0.15 No Hit ACCGCGGTCTCCAACCATGGTTCCATCAAGCCATTCAGGTGGGGATGCAC 6 0.15 No Hit CCCAGAATTCAAATTCAAACCCTAATTACCATTTACCGATACCCAAGACT 5 0.125 No Hit CTCCATTGACATCTTCTGCTGTAATGCCCTTCTTCTCAACATTCACAACA 5 0.125 No Hit CTTAGATCCTCAACAAGCTCATTGAGGAGAATATATGCCAGTGACAAAGC 5 0.125 No Hit GCCCATAACAATAGGAAATATCAGAAATCCAATAAGAATCAGCAATTCAG 5 0.125 No Hit CTCCTGTGCAGAAACCTCTTCACCAGCAAAACTTCTTTTTCTGAAAACTT 5 0.125 No Hit CTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCT 5 0.125 No Hit CTCCTTTATAAACTTTTCCAAATCCTCCTTGTCCAAGGATATTTTCCTCA 5 0.125 No Hit AGCCAATCGAACAAAGCTAGTTATGGTAAAAAGAGTAATTCCACTAGAAA 5 0.125 No Hit GGTGAATCCAACTGAGAGTCATTTAGATCAGATGCAAGTCCTTTTGGGGA 5 0.125 No Hit TCTTAATCTTTGCTGCATGGTCGAGATTGCTGATGGTATCAAAGAGCTTT 5 0.125 No Hit TCCCCATCTGTCCTTGTCGGTCTGCTTCTTCTCATCGTACTCTGCAACAA 5 0.125 No Hit AGGGGATGAGAGCGTGTGGGGTTGACTATTTTTCTTAAATTGGATTAATC 5 0.125 No Hit CCTAAACTCAACTTCTCCCTCTGTGGTAAAGTGTGTGTATGCAAGTGGAT 5 0.125 No Hit TCTACACATAATTTAAAGCGTAATATCTCGTAATTTGATACTCCTAAAAT 5 0.125 No Hit TTCCGTTGTGCCCTTAAAAGGGAGGATCATCTTGGTCCAGACAATTCATC 5 0.125 No Hit CTTGCATAGACTTTAAGAGTTGGTTCAAGACGAGTGGCCCAACAAATTGG 5 0.125 No Hit TGCTCGAGGCATGGCTACAATGACAGCTGAAAGAAGGGAATAACCAGCAC 5 0.125 No Hit CTCCCAACTAAGATTTTCAGCAATTCCATTTACTTTCTCAAGTGCTTGAT 5 0.125 No Hit CCCATGTCCAAATCCTACTCGTCGTCGTCATCCTCCTCTGCACTGCCCTC 5 0.125 No Hit CATGAAATTAATCGATAATTAAGTATATTGTACGAACTAGCCACAACACT 5 0.125 No Hit GTTCAAGAAGTCCTGCTCTGCGAATGGCGTAGGCGGAGTGACCTTGAGAG 5 0.125 No Hit GGCTGCAACGTAAAATGAGAACTAATCATCTCTACGACTAATTTGCACTT 5 0.125 No Hit CTCTTTCACTTCCTCTGCTTGAGGATAAGAATATTCAGTTGCAACTGCAA 5 0.125 No Hit GGCCAATTGCCTTTGAGACAAATGGAATGGTACGAGAAGCACGAGGATTT 5 0.125 No Hit CCAGCCTTGTTGATCAACATGTAGGTGTTAACACCAGAGCCTTCCATATG 5 0.125 No Hit CTTCCTTGAGGCATACCCTGCAATTGCAATTACCACGACAAAATGGGCAT 5 0.125 No Hit ATCAAGTTCTGGGACGACTTAGACACCTTTCGGCTTGGCGGCAATAAAGC 5 0.125 No Hit GCTGCATTTGGGTCGGTACAGCCTTCAGGGATAGGCACCTTTACTTGCTG 5 0.125 No Hit GGGAAGAACATGTGTGGTATTGCAACCTGTGCATCATATCCTGTCGTTGC 5 0.125 No Hit TGTAGATTTGCTTAACAATTCCTTGCCTATCCTTCAATGGACCTTCCAAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0625 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.1875 0.0 0.0 0.0 0.0 80-81 0.3125 0.0 0.0 0.0 0.0 82-83 0.45 0.0 0.0 0.0 0.0 84-85 0.55 0.0 0.0 0.0 0.0 86-87 0.6875 0.0 0.0 0.0 0.0 88-89 0.9 0.0 0.0 0.0 0.0 90-91 1.075 0.0 0.0 0.0 0.0 92-93 1.25 0.0 0.0 0.0 0.0 94-95 1.4625 0.0 0.0 0.0 0.0 96-97 1.9375 0.0 0.0 0.0 0.0 98-99 2.3625 0.0 0.0 0.0 0.0 100-101 2.6875 0.0 0.0 0.0 0.0 102-103 2.9124999999999996 0.0 0.0 0.0 0.0 104-105 3.3 0.0 0.0 0.0 0.0 106-107 3.625 0.0 0.0 0.0 0.0 108-109 4.1375 0.0 0.0 0.0 0.0 110-111 4.45 0.0 0.0 0.0 0.0 112-113 4.8875 0.0 0.0 0.0 0.0 114-115 5.4125 0.0 0.0 0.0 0.0 116-117 5.9375 0.0 0.0 0.0 0.0 118-119 6.45 0.0 0.0 0.0 0.0 120-121 6.887499999999999 0.0 0.0 0.0 0.0 122-123 7.1875 0.0 0.0 0.0 0.0 124-125 8.0875 0.0 0.0 0.0 0.0 126-127 9.0125 0.0 0.0 0.0 0.0 128-129 9.8875 0.0 0.0 0.0 0.0 130-131 10.4875 0.0 0.0 0.0 0.0 132-133 11.1625 0.0 0.0 0.0 0.0 134-135 11.7625 0.0 0.0 0.0 0.0 136-137 12.3625 0.0 0.0 0.0 0.0 138-139 13.3625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR13695452 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695452_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.08075 37.0 37.0 37.0 37.0 37.0 2 36.041 37.0 37.0 37.0 37.0 37.0 3 36.1045 37.0 37.0 37.0 37.0 37.0 4 36.1905 37.0 37.0 37.0 37.0 37.0 5 36.2195 37.0 37.0 37.0 37.0 37.0 6 36.074 37.0 37.0 37.0 37.0 37.0 7 36.1415 37.0 37.0 37.0 37.0 37.0 8 36.289 37.0 37.0 37.0 37.0 37.0 9 36.2135 37.0 37.0 37.0 37.0 37.0 10-14 36.183400000000006 37.0 37.0 37.0 37.0 37.0 15-19 36.216899999999995 37.0 37.0 37.0 37.0 37.0 20-24 36.1574 37.0 37.0 37.0 37.0 37.0 25-29 36.072050000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.101099999999995 37.0 37.0 37.0 37.0 37.0 35-39 36.00295 37.0 37.0 37.0 37.0 37.0 40-44 35.9768 37.0 37.0 37.0 37.0 37.0 45-49 36.057 37.0 37.0 37.0 37.0 37.0 50-54 35.9842 37.0 37.0 37.0 37.0 37.0 55-59 35.96195 37.0 37.0 37.0 37.0 37.0 60-64 35.92075 37.0 37.0 37.0 37.0 37.0 65-69 35.85615 37.0 37.0 37.0 37.0 37.0 70-74 35.84905 37.0 37.0 37.0 37.0 37.0 75-79 35.85235 37.0 37.0 37.0 37.0 37.0 80-84 35.837500000000006 37.0 37.0 37.0 37.0 37.0 85-89 35.733700000000006 37.0 37.0 37.0 37.0 37.0 90-94 35.76715 37.0 37.0 37.0 37.0 37.0 95-99 35.7688 37.0 37.0 37.0 37.0 37.0 100-104 35.70185 37.0 37.0 37.0 37.0 37.0 105-109 35.651399999999995 37.0 37.0 37.0 37.0 37.0 110-114 35.60315 37.0 37.0 37.0 37.0 37.0 115-119 35.54535 37.0 37.0 37.0 37.0 37.0 120-124 35.441449999999996 37.0 37.0 37.0 37.0 37.0 125-129 35.51175 37.0 37.0 37.0 37.0 37.0 130-134 35.310449999999996 37.0 37.0 37.0 29.8 37.0 135-139 35.2955 37.0 37.0 37.0 34.6 37.0 140-144 35.09925 37.0 37.0 37.0 25.0 37.0 145-149 34.9088 37.0 37.0 37.0 25.0 37.0 150-151 34.59675 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.0 15 2.0 16 0.0 17 1.0 18 0.0 19 2.0 20 0.0 21 3.0 22 0.0 23 2.0 24 5.0 25 4.0 26 10.0 27 10.0 28 18.0 29 13.0 30 32.0 31 61.0 32 81.0 33 127.0 34 255.0 35 685.0 36 2516.0 37 170.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.832038104788168 14.865881173226372 16.47029330659313 38.83178741539233 2 29.15 22.875 32.875 15.1 3 20.125 29.525000000000002 30.275000000000002 20.075000000000003 4 24.025 30.3 24.75 20.925 5 25.900000000000002 34.475 22.275 17.349999999999998 6 22.475 37.724999999999994 23.200000000000003 16.6 7 19.525000000000002 21.3 39.4 19.775000000000002 8 20.200000000000003 26.424999999999997 30.65 22.725 9 22.7 24.725 31.95 20.625 10-14 22.98 29.995 26.805 20.22 15-19 22.97 28.265 28.01 20.755000000000003 20-24 22.71135567783892 28.59929964982491 28.189094547273637 20.50025012506253 25-29 22.827120340255192 28.43632724543407 27.690768076057044 21.04578433825369 30-34 22.428457074244545 28.50210126075645 27.98178907344407 21.087652591554935 35-39 22.437340537295512 28.58572214718095 27.650207614187806 21.326729701335733 40-44 23.088853311987194 27.04622773664199 29.01741044626776 20.847508505103065 45-49 22.803682209325597 27.40644386631979 28.95737442465479 20.832499499699818 50-54 23.526763381690845 27.963981990995496 27.583791895947975 20.92546273136568 55-59 23.23742807105329 27.455591693770327 28.886664998749062 20.42031523642732 60-64 23.678022912601932 27.565160838461157 28.470658862374304 20.286157386562607 65-69 23.023058070324616 28.880108037813233 26.889411293952886 21.207422597909268 70-74 23.252439329497125 28.426319739804857 27.695771828871653 20.62546910182637 75-79 22.624706058938308 27.963176064441885 27.622954920698454 21.78916295592135 80-84 23.65182591295648 28.199099549774886 27.92896448224112 20.22011005502751 85-89 22.991093765635945 29.180426298408886 26.638647052937053 21.189832883018113 90-94 22.957626694682077 28.965931262194207 26.8447646205413 21.231677422582422 95-99 23.98199099549775 28.209104552276138 28.23411705852926 19.574787393696848 100-104 24.423317488116087 27.66574931198399 27.91593695271454 19.99499624718539 105-109 24.111878314820373 28.760132092464723 27.44921445011508 19.678775142599818 110-114 24.47346040322177 28.425634098754315 27.1849517234479 19.915953774576018 115-119 24.450893080502325 28.328413468754693 27.487867113623853 19.73282633711913 120-124 25.37403052289217 28.04603452589442 26.730047535651742 19.84988741556167 125-129 24.786110972131887 28.068244358833244 27.447841096712867 19.69780357232201 130-134 25.77190612020217 27.08802482109793 26.947905719861883 20.19216333883801 135-139 26.991593274619696 26.74139311449159 26.556244995996796 19.71076861489191 140-144 26.294461954074745 27.485116814247835 27.355045274901197 18.865375956776226 145-149 27.446957566052845 27.256805444355486 26.546236989591677 18.75 150-151 28.353353353353356 27.05205205205205 25.88838838838839 18.706206206206204 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.5 18 0.5 19 0.5 20 0.5 21 0.5 22 2.5 23 3.0 24 2.0 25 1.0 26 5.0 27 6.0 28 6.5 29 14.5 30 18.0 31 17.0 32 21.5 33 38.5 34 55.5 35 69.5 36 87.5 37 114.0 38 145.0 39 175.0 40 196.5 41 238.0 42 272.0 43 285.0 44 289.5 45 279.5 46 250.5 47 214.5 48 218.5 49 216.0 50 170.5 51 129.0 52 96.5 53 67.5 54 60.5 55 62.0 56 51.5 57 35.5 58 25.5 59 14.5 60 12.0 61 9.5 62 5.0 63 2.0 64 0.5 65 2.5 66 2.0 67 0.0 68 0.5 69 0.5 70 0.0 71 0.0 72 0.5 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.5 81 0.5 82 0.0 83 0.0 84 0.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27499999999999997 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.05 25-29 0.075 30-34 0.06 35-39 0.055 40-44 0.06 45-49 0.06 50-54 0.05 55-59 0.075 60-64 0.055 65-69 0.034999999999999996 70-74 0.075 75-79 0.065 80-84 0.05 85-89 0.06999999999999999 90-94 0.055 95-99 0.05 100-104 0.075 105-109 0.06999999999999999 110-114 0.055 115-119 0.065 120-124 0.075 125-129 0.065 130-134 0.08499999999999999 135-139 0.08 140-144 0.055 145-149 0.08 150-151 0.1 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.45 #Duplication Level Percentage of deduplicated Percentage of total 1 72.67818574514038 50.475 2 17.278617710583152 24.0 3 6.19150467962563 12.9 4 2.087832973362131 5.800000000000001 5 1.2239020878329734 4.25 6 0.25197984161267095 1.05 7 0.14398848092152627 0.7000000000000001 8 0.10799136069114472 0.6 9 0.03599712023038157 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TACAGAGTGGGTGGAGGGCCACTTGGCGAAGGACTTGACCCCATTTATCC 9 0.22499999999999998 No Hit ACACTCCTCTAGCTGTCAATGCATAAAAGGGCATCTAAGTGAAGATGTGA 8 0.2 No Hit CAGAGTTTTGTTACTTTCATTGCGAGGGAAACGAAAGTAATGTTAGAGGG 8 0.2 No Hit CGTCCATCAAGTTCTTACAACTCTCCTTTCTTCACTACAAATTCTGGTGC 8 0.2 No Hit CTTTCTGCAAGTCTTGGTGGAAATTAGGGAGTCCCATTTTGCAGCCACTT 7 0.17500000000000002 No Hit AGACTGGCTTGCTAATCTGGGCTGTTACCCTAGCTGGCATTCTTGCCGGA 7 0.17500000000000002 No Hit CCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACT 7 0.17500000000000002 No Hit ATCTAGTTAGATGTAATATCAAACAGAAGCTATATTTTTTATTCCCTCTT 7 0.17500000000000002 No Hit GGACAGGACATGAGACATCAGATATTGCTTCTGTAATGGATGGCGAATTG 6 0.15 No Hit CTTGCATTCTTCTGTTTCTCGCTTCCTCCACTGATAAAACCTCCCTCTCT 6 0.15 No Hit CAGAGATTGTCAAGACAGTTCAAGAGGCTTGGGATAAAGTTGAAGACAAG 6 0.15 No Hit CGACTGCTGTTGCCGCGTCCTATTTTTCGGGGACCCGAACTCAATACACA 6 0.15 No Hit GGAATCTGAAACGGAAAAAAAGTGACAAAAAATTAAGTAGGAGCATCAAA 6 0.15 No Hit GTTAAAGCCAATTAAGGTGGGGCAATCTTCCTCTCTGAGAGTTGGCCAGC 6 0.15 No Hit GGAGTGGGTAAAAAAGGACAAGCGTAGAATGCTACATGTTGTTTATCGTG 6 0.15 No Hit TGAAGGCCTACAACAAGGAGCAGGCTGAAGGGCCCAAGGAAGAAGAGGAA 5 0.125 No Hit GCTCTACTGGCAAAGCTTGGCATTAGGCCATTTTCTTATGGTCTGGTTGT 5 0.125 No Hit GGAAAGATGGGGCTCTCATTCACCAAACTTTTTAGCCGTCTGTTTGCCAA 5 0.125 No Hit TAACGAACTTTCTCGGAAAAGCAGAGAACCACGCCGTAGAGGCCAAAAGC 5 0.125 No Hit GTGAGAAGAAAATAACAAAGAAAACTAAAACTGAGAAGTATTGGGATTGG 5 0.125 No Hit CTGGACTATGTGGAAGCTACCCATGTTTGGATGCACTGAGGCATCTCAAG 5 0.125 No Hit TGAGCTTGAGTGCATTCTTCTAAGTAAAAGAAATCCCTTAATTTCATCAC 5 0.125 No Hit AAGAAAAGAAGAGAAGAAGGGAAAGTAAGAAGAAAAAGAAGGATTTCGAG 5 0.125 No Hit AGCTGAGACTATGTTGCGTTTGGTGCCTGTGGCTTTGTGTGTCTCAGCAC 5 0.125 No Hit TTTCTAACCATCATGCTTCTCACTCAAAGGCTTTGCACGAAGCTTTGATC 5 0.125 No Hit GCAGACAATTTTAAAGGAGCTTGTTTTGGATAAGGTTTACTCTTCTTGCT 5 0.125 No Hit CGGCCGCAAAGATTTTGGTCTTATTGTTGGTATGGAGAAAGATGAATCTT 5 0.125 No Hit CTCCATAATTAAGGGAGTACAAAGTAGATTATGAAGAAGGTTTGCTGCAG 5 0.125 No Hit CAGGGATATTCTGGTTGAGCAAACTGGTCACTGGAGCAATATTAAGAAGA 5 0.125 No Hit TAACGGTATTTGCATTATTGATGGTTTTTACTATGTTGGGTATGCTGTTC 5 0.125 No Hit GTTTCTGTGAGAAAACATGGAGCCACACTCCACAGTACAAGATTGGCTAT 5 0.125 No Hit CTCCCTCAAGCATGTGATTGATCATCTTCTCCACAATGTCCTGTGCCAAT 5 0.125 No Hit TCTGCATTTGCTAGCTAGAAGTCACTCTACCCTTCGCATTACTTCCTCAA 5 0.125 No Hit TGATCATGAAGTCTCTACTTCAGCACATCCTTCATCTCAGCCAATTGTTG 5 0.125 No Hit CTGGACAGAGGGCTTTTGATCTTGCTCGGCTTGCAAATGACGATGATGTA 5 0.125 No Hit CTTGTTGATATAATCAAGGCACAGATTCAGAGGGATGTGGACAAGCGGAT 5 0.125 No Hit GCCGTTGCGAGCTCTATCGAGGAAGAAGTTTATAAGGTTCAATGGGCAAC 5 0.125 No Hit CGTGGATTTGCAGTGAAGTTTTACACCAGAGAGGGTAACTTTGATCTCGT 5 0.125 No Hit GTTCGGGGATGGTTTACTGACTTGCGTGACCGCACTGCATCATCTCTTAT 5 0.125 No Hit GAGCATTGGAAAGAAGCTTGTGAACTCAAGGGAAGGACCCCCAACTTTTG 5 0.125 No Hit TGGTGGGGAGGCTTCTGGAAGATTGGCAATGATGCTTCCCAATTTGTTGG 5 0.125 No Hit TTTAAGCTTAATCAATCAATCATCATGTCTAGCACCTGCGACACCTGCGA 5 0.125 No Hit GGGAGACACTAGATTGGTTGGTTGCTTGGAGAATGAAGGCTTCGAAGTCC 5 0.125 No Hit GAAGGTGAATCGAAAGGAATTGTGGTCATATTCGCATGGAATTCGATTCC 5 0.125 No Hit CTTCGTTTTGACCTCAACACTGTTATCTCTGCTAAGTCCAAGGACGAGAA 5 0.125 No Hit CGTACATCAACACACATGGGCATGCACCTTGGATGCAAATTCCTATTACA 5 0.125 No Hit GTTAATGGAGAACGGGTTATACACCCTGAGACTTTTTAAAATAATTTTCT 5 0.125 No Hit CAATTTTACAGGAAACCACTTAAATTGTGGTGGGCTGAATTTGCATCTCT 5 0.125 No Hit TGTTTGCAGAGCAGCACATGCCGACAGCTGCTGCAATGCTTTCTTCCTTG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0625 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.16249999999999998 0.0 0.0 0.0 0.0 80-81 0.2875 0.0 0.0 0.0 0.0 82-83 0.425 0.0 0.0 0.0 0.0 84-85 0.525 0.0 0.0 0.0 0.0 86-87 0.6625 0.0 0.0 0.0 0.0 88-89 0.875 0.0 0.0 0.0 0.0 90-91 1.05 0.0 0.0 0.0 0.0 92-93 1.225 0.0 0.0 0.0 0.0 94-95 1.4375 0.0 0.0 0.0 0.0 96-97 1.925 0.0 0.0 0.0 0.0 98-99 2.3625 0.0 0.0 0.0 0.0 100-101 2.7 0.0 0.0 0.0 0.0 102-103 2.9375 0.0 0.0 0.0 0.0 104-105 3.325 0.0 0.0 0.0 0.0 106-107 3.65 0.0 0.0 0.0 0.0 108-109 4.1125 0.0 0.0 0.0 0.0 110-111 4.4125 0.0 0.0 0.0 0.0 112-113 4.9625 0.0 0.0 0.0 0.0 114-115 5.525 0.0 0.0 0.0 0.0 116-117 6.0875 0.0 0.0 0.0 0.0 118-119 6.6 0.0 0.0 0.0 0.0 120-121 7.0375 0.0 0.0 0.0 0.0 122-123 7.3625 0.0 0.0 0.0 0.0 124-125 8.2875 0.0 0.0 0.0 0.0 126-127 9.2 0.0 0.0 0.0 0.0 128-129 10.1125 0.0 0.0 0.0 0.0 130-131 10.7625 0.0 0.0 0.0 0.0 132-133 11.4625 0.0 0.0 0.0 0.0 134-135 12.0625 0.0 0.0 0.0 0.0 136-137 12.662500000000001 0.0 0.0 0.0 0.0 138-139 13.649999999999999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGCTCA 10 0.006830828 145.0 3 ATAACAT 10 0.006830828 145.0 6 CCCCCCC 35 0.0035366106 20.714287 110-114 >>END_MODULE Read 894911 spots for SRR13695452.sra Written 894911 spots for SRR13695452.sra Read 894911 spots for SRR13695452.sra Written 894911 spots for SRR13695452.sra Read 894911 spots for SRR13695452.sra Written 894911 spots for SRR13695452.sra Read 894911 spots for SRR13695452.sra Written 894911 spots for SRR13695452.sra Read 894911 spots for SRR13695452.sra Written 894911 spots for SRR13695452.sra Read 894911 spots for SRR13695452.sra Written 894911 spots for SRR13695452.sra Read 894911 spots for SRR13695452.sra Written 894911 spots for SRR13695452.sra Read 894911 spots for SRR13695452.sra Written 894911 spots for SRR13695452.sra Read 894911 spots for SRR13695452.sra Written 894911 spots for SRR13695452.sra Read 894911 spots for SRR13695452.sra Written 894911 spots for SRR13695452.sra Read 894911 spots for SRR13695452.sra Written 894911 spots for SRR13695452.sra Read 894911 spots for SRR13695452.sra Written 894911 spots for SRR13695452.sra Read 894911 spots for SRR13695452.sra Written 894911 spots for SRR13695452.sra Read 894911 spots for SRR13695452.sra Written 894911 spots for SRR13695452.sra Read 894911 spots for SRR13695452.sra Written 894911 spots for SRR13695452.sra Read 894911 spots for SRR13695452.sra Written 894911 spots for SRR13695452.sra Read 894911 spots for SRR13695452.sra Written 894911 spots for SRR13695452.sra Read 894911 spots for SRR13695452.sra Written 894911 spots for SRR13695452.sra Read 894929 spots for SRR13695452.sra Written 894929 spots for SRR13695452.sra Read 894911 spots for SRR13695452.sra Written 894911 spots for SRR13695452.sra SRR ids: ['SRR13695452.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_f60wxktz SRR13695452.sra spots: 17898238 blocks: [[1, 894911], [894912, 1789822], [1789823, 2684733], [2684734, 3579644], [3579645, 4474555], [4474556, 5369466], [5369467, 6264377], [6264378, 7159288], [7159289, 8054199], [8054200, 8949110], [8949111, 9844021], [9844022, 10738932], [10738933, 11633843], [11633844, 12528754], [12528755, 13423665], [13423666, 14318576], [14318577, 15213487], [15213488, 16108398], [16108399, 17003309], [17003310, 17898238]] SRR13695452 file size 6060903 SRR13695452 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695452 SRR13695452_1.fastq SRR13695452_2.fastq Input file: SRR13695452_1.fastq Paired file: SRR13695452_2.fastq trimmed: SRR13695452-trimmed-pair1.fastq, SRR13695452-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 04:27:00 2025 >> started Wed Feb 12 04:27:22 2025 >> done (21.446s) 17898238 read pairs processed; of these: 152 ( 0.00%) short read pairs filtered out after trimming by size control 1915 ( 0.01%) empty read pairs filtered out after trimming by size control 17896171 (99.99%) read pairs available; of these: 2918683 (16.31%) trimmed read pairs available after processing 14977488 (83.69%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 0 0.00% 20 3 0.00% 21 7 0.00% 22 3 0.00% 23 6 0.00% 24 3 0.00% 25 0 0.00% 26 4 0.00% 27 11 0.00% 28 10 0.00% 29 5 0.00% 30 6 0.00% 31 9 0.00% 32 4 0.00% 33 26 0.00% 34 10 0.00% 35 13 0.00% 36 22 0.00% 37 19 0.00% 38 21 0.00% 39 28 0.00% 40 42 0.00% 41 55 0.00% 42 57 0.00% 43 49 0.00% 44 80 0.00% 45 83 0.00% 46 66 0.00% 47 87 0.00% 48 173 0.00% 49 151 0.00% 50 222 0.00% 51 235 0.00% 52 217 0.00% 53 266 0.00% 54 340 0.00% 55 326 0.00% 56 298 0.00% 57 398 0.00% 58 490 0.00% 59 629 0.00% 60 743 0.00% 61 902 0.01% 62 959 0.01% 63 1147 0.01% 64 1322 0.01% 65 1427 0.01% 66 1496 0.01% 67 1675 0.01% 68 1892 0.01% 69 2366 0.01% 70 2655 0.01% 71 3157 0.02% 72 3581 0.02% 73 3989 0.02% 74 4375 0.02% 75 4982 0.03% 76 5275 0.03% 77 5612 0.03% 78 6105 0.03% 79 6940 0.04% 80 7636 0.04% 81 8375 0.05% 82 9953 0.06% 83 10455 0.06% 84 12201 0.07% 85 12688 0.07% 86 13188 0.07% 87 13585 0.08% 88 14835 0.08% 89 15589 0.09% 90 16282 0.09% 91 17452 0.10% 92 18812 0.11% 93 20278 0.11% 94 21568 0.12% 95 22994 0.13% 96 23334 0.13% 97 24533 0.14% 98 25283 0.14% 99 25724 0.14% 100 26756 0.15% 101 27392 0.15% 102 28914 0.16% 103 30326 0.17% 104 31966 0.18% 105 33097 0.18% 106 34232 0.19% 107 35300 0.20% 108 35666 0.20% 109 36350 0.20% 110 37109 0.21% 111 37418 0.21% 112 38819 0.22% 113 39723 0.22% 114 41217 0.23% 115 42958 0.24% 116 44312 0.25% 117 44699 0.25% 118 45560 0.25% 119 46287 0.26% 120 47198 0.26% 121 46691 0.26% 122 48074 0.27% 123 48601 0.27% 124 49672 0.28% 125 51271 0.29% 126 52158 0.29% 127 53639 0.30% 128 54096 0.30% 129 53898 0.30% 130 54891 0.31% 131 54630 0.31% 132 54259 0.30% 133 56092 0.31% 134 56891 0.32% 135 57335 0.32% 136 58845 0.33% 137 60256 0.34% 138 60477 0.34% 139 61738 0.34% 140 61099 0.34% 141 60994 0.34% 142 61715 0.34% 143 62067 0.35% 144 62837 0.35% 145 63852 0.36% 146 63660 0.36% 147 64388 0.36% 148 66043 0.37% 149 66087 0.37% 150 67287 0.38% 151 14977488 83.69% 17896171 reads passed initial QC criterion=sequence-density sequence-density=0.38 sequence-density-rank=1 fanout-score=2.55 fanout-score-rank=23 prefix-density=0.42 prefix-fanout=2.3 sequence=TGAGATGCCTCAGTGCATCCAAACATGGGTAG criterion=fanout-score sequence-density=0.01 sequence-density-rank=33 fanout-score=331.41 fanout-score-rank=1 prefix-density=0.26 prefix-fanout=16.3 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGAGTATGAATGTGTTCTCTG criterion=sequence-density sequence-density=0.55 sequence-density-rank=1 fanout-score=2.16 fanout-score-rank=27 prefix-density=0.57 prefix-fanout=2.1 sequence=TGTAAGAGATGGCTTCCTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=12.73 fanout-score-rank=1 prefix-density=0.07 prefix-fanout=1.0 sequence=GCTACACAGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAATCATCATGTCTAGCACCTGCGACACCTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAGTGAACAAACAAAAGTGTCCGTGAGTGTCTTTATCTTTGTGTCTTCTTCA SRR13695452 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 04:28:03 Started mapping on | Feb 12 04:28:04 Finished on | Feb 12 04:30:08 Mapping speed, Million of reads per hour | 519.57 Number of input reads | 17896171 Average input read length | 292 UNIQUE READS: Uniquely mapped reads number | 16688331 Uniquely mapped reads % | 93.25% Average mapped length | 291.28 Number of splices: Total | 16398773 Number of splices: Annotated (sjdb) | 16035290 Number of splices: GT/AG | 16048177 Number of splices: GC/AG | 277732 Number of splices: AT/AC | 8987 Number of splices: Non-canonical | 63877 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 2.95 Insertion rate per base | 0.02% Insertion average length | 2.36 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 396226 % of reads mapped to multiple loci | 2.21% Number of reads mapped to too many loci | 74734 % of reads mapped to too many loci | 0.42% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.02% % of reads unmapped: other | 0.10% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 811836 811836 811836 N_multimapping 396226 396226 396226 N_noFeature 702995 16243342 1002748 N_ambiguous 251069 1966 104222 UnstrandedReadsAssigned:15734267 PositiveStrandReadsAssigned:443023 NegativeStrandReadsAssigned:15581361 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695452 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695452-trimmed-pair1.fastq SRR13695452-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 17,896,171 reads, 15,605,259 reads pseudoaligned [quant] estimated average fragment length: 237.965 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,208 rounds 52401 SRR13695452.ke.tsv 34699 SRR13695452.se.tsv 87100 total ==> SRR13695452.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1781.04 473 17.0542 Potri.005G024800.1.v4.1 1035 798.035 218 17.5419 Potri.004G059700.1.v4.1 961 724.08 16 1.41898 Potri.007G009000.2.v4.1 1416 1179.04 0 0 Potri.003G141000.2.v4.1 2943 2706.04 921.517 21.8682 Potri.016G087400.1.v4.1 270 93.2492 716 493.072 Potri.015G069301.1.v4.1 564 334.61 0 0 Potri.010G195200.1.v4.1 1773 1536.04 104 4.34785 Potri.012G127500.1.v4.1 977 740.045 55 4.77251 ==> SRR13695452.se.tsv <== Potri.001G166300.v4.1 2 Potri.001G448400.v4.1 146 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 225 Potri.001G212900.v4.1 7 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 2 SRR13695452 completed mapping pipeline successfully