Starting /dee2/code/volunteer_pipeline.sh SRR13695454 current disk space = 3049022267392 free memory = 744065680 SRR13695454 SRAfilesize a3a1a10095e111ef5e6f3394d892feed SRR13695454.sra SRR13695454.sra file validated SRR13695454 is paired end SRR13695454 is conventional basespace SRR13695454 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695454_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.6045 37.0 37.0 37.0 37.0 37.0 2 36.22575 37.0 37.0 37.0 37.0 37.0 3 36.5335 37.0 37.0 37.0 37.0 37.0 4 36.544 37.0 37.0 37.0 37.0 37.0 5 36.608 37.0 37.0 37.0 37.0 37.0 6 36.546 37.0 37.0 37.0 37.0 37.0 7 36.446 37.0 37.0 37.0 37.0 37.0 8 36.499 37.0 37.0 37.0 37.0 37.0 9 36.5845 37.0 37.0 37.0 37.0 37.0 10-14 36.56 37.0 37.0 37.0 37.0 37.0 15-19 36.5366 37.0 37.0 37.0 37.0 37.0 20-24 36.4747 37.0 37.0 37.0 37.0 37.0 25-29 36.472500000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.4584 37.0 37.0 37.0 37.0 37.0 35-39 36.4193 37.0 37.0 37.0 37.0 37.0 40-44 36.371300000000005 37.0 37.0 37.0 37.0 37.0 45-49 36.3361 37.0 37.0 37.0 37.0 37.0 50-54 36.34740000000001 37.0 37.0 37.0 37.0 37.0 55-59 36.32379999999999 37.0 37.0 37.0 37.0 37.0 60-64 36.3063 37.0 37.0 37.0 37.0 37.0 65-69 36.2915 37.0 37.0 37.0 37.0 37.0 70-74 36.2836 37.0 37.0 37.0 37.0 37.0 75-79 36.28529999999999 37.0 37.0 37.0 37.0 37.0 80-84 36.2044 37.0 37.0 37.0 37.0 37.0 85-89 36.194900000000004 37.0 37.0 37.0 37.0 37.0 90-94 36.0635 37.0 37.0 37.0 37.0 37.0 95-99 36.0652 37.0 37.0 37.0 37.0 37.0 100-104 36.072199999999995 37.0 37.0 37.0 37.0 37.0 105-109 36.0433 37.0 37.0 37.0 37.0 37.0 110-114 36.026300000000006 37.0 37.0 37.0 37.0 37.0 115-119 35.9918 37.0 37.0 37.0 37.0 37.0 120-124 35.8986 37.0 37.0 37.0 37.0 37.0 125-129 35.866400000000006 37.0 37.0 37.0 37.0 37.0 130-134 35.860400000000006 37.0 37.0 37.0 37.0 37.0 135-139 35.8432 37.0 37.0 37.0 37.0 37.0 140-144 35.8202 37.0 37.0 37.0 37.0 37.0 145-149 35.5363 37.0 37.0 37.0 37.0 37.0 150-151 35.51 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 2.0 22 2.0 23 1.0 24 5.0 25 0.0 26 3.0 27 8.0 28 10.0 29 18.0 30 25.0 31 41.0 32 57.0 33 79.0 34 175.0 35 304.0 36 2966.0 37 304.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.15 12.1 8.4 38.35 2 19.276200050263885 13.59638100025132 36.26539331490324 30.862025634581553 3 18.15 17.125 25.7 39.025 4 22.625 22.8 22.8 31.775 5 24.474999999999998 30.225 25.275 20.025000000000002 6 20.775 33.725 23.3 22.2 7 15.575 28.349999999999998 39.85 16.225 8 17.95 27.150000000000002 31.075000000000003 23.825 9 17.05 23.95 36.15 22.85 10-14 19.095000000000002 29.87 28.035 23.0 15-19 19.855 29.17 27.625 23.35 20-24 19.575 30.435000000000002 26.88 23.11 25-29 20.105 29.37 27.584999999999997 22.939999999999998 30-34 19.915 28.825 27.439999999999998 23.82 35-39 20.025000000000002 28.68 27.965 23.330000000000002 40-44 20.555 28.645 27.944999999999997 22.855 45-49 20.205000000000002 27.894999999999996 28.225 23.674999999999997 50-54 19.86 28.285 28.360000000000003 23.494999999999997 55-59 20.115 28.515 27.939999999999998 23.43 60-64 20.195 27.994999999999997 28.43 23.380000000000003 65-69 19.085 28.465 28.565 23.885 70-74 19.82 28.68 28.055000000000003 23.445 75-79 20.205000000000002 28.444999999999997 28.360000000000003 22.99 80-84 19.935 27.92 28.044999999999998 24.099999999999998 85-89 20.630000000000003 27.92 28.044999999999998 23.405 90-94 20.169999999999998 28.37 28.08 23.380000000000003 95-99 20.53 28.48 27.639999999999997 23.35 100-104 20.255000000000003 29.035 27.775 22.935 105-109 20.724999999999998 28.465 27.665 23.145 110-114 20.655 28.87 27.33 23.145 115-119 21.105 28.915000000000003 26.775 23.205000000000002 120-124 21.02 28.310000000000002 26.540000000000003 24.13 125-129 20.325 28.62 26.815 24.240000000000002 130-134 21.02 28.07 27.825 23.085 135-139 20.685000000000002 29.38 26.935 23.0 140-144 21.48 28.54 26.534999999999997 23.445 145-149 20.955 27.834999999999997 27.034999999999997 24.175 150-151 20.0375 29.325000000000003 26.9625 23.674999999999997 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 0.5 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.5 19 1.0 20 1.0 21 0.5 22 1.5 23 2.5 24 1.5 25 2.5 26 8.0 27 13.0 28 13.5 29 20.5 30 24.0 31 25.0 32 33.0 33 37.0 34 47.5 35 61.0 36 93.0 37 113.5 38 128.0 39 169.0 40 189.0 41 204.0 42 247.0 43 280.0 44 260.0 45 267.0 46 279.5 47 256.0 48 218.0 49 200.5 50 197.5 51 164.0 52 112.0 53 76.5 54 64.5 55 46.0 56 40.0 57 28.0 58 17.0 59 13.5 60 11.0 61 8.5 62 6.5 63 5.0 64 3.0 65 3.5 66 1.5 67 0.0 68 0.5 69 1.0 70 0.5 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.525 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.2 #Duplication Level Percentage of deduplicated Percentage of total 1 69.97800586510265 47.725 2 19.244868035190617 26.25 3 6.928152492668621 14.174999999999999 4 2.309384164222874 6.3 5 1.2463343108504399 4.25 6 0.18328445747800587 0.75 7 0.07331378299120235 0.35000000000000003 8 0.036656891495601175 0.2 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGGATGATCCACTGTAACTCATGCATCAACAGCGAGAGCTGCTGCGCTTC 8 0.2 No Hit CACCGAGGTTTATTCAGGTAGGAAAACTGTCTAAGATCATGACTTGATGC 7 0.17500000000000002 No Hit GAACCATCAACTCAGCAACCGAGGACACCTCAGCTTTTATGGATTTGTAA 7 0.17500000000000002 No Hit CCCTCAATCAGCTCCACCTTTCCTGCATCTTGCCATGCAACACCGGTGAG 6 0.15 No Hit GGGAGGAGATATAGAATACAGAACTAAAAAGGAATCCTTGGCAGTGTTTC 6 0.15 No Hit GCCCTGGTGCTTTGAGCTCACAGCTTTTACCACTAGATAATGGACCCAAA 6 0.15 No Hit CTTCTGACCGTCCTTCACTTGAACGCCACCACTGTTGGCGCTCATCTGCA 6 0.15 No Hit GCACGAGGAATGGTGATTATGAAAATAATATGAGCTAGCTGACATACTGC 6 0.15 No Hit CCACATACCTACCTCCAGCAACTAATGCATAATATTTGCTTTACTTCCAA 5 0.125 No Hit CCTTTTACTTCCATGTATGTCTTGCTCTGGATTTCTTCATAGGTCAGCCT 5 0.125 No Hit GCTACGTAACCAAAACCAGAAATGCTTGACATGCCTCAAACCATGGATAA 5 0.125 No Hit GGGGTAAATTTTATTAGCAGCACAGAAGTCCAACATCTCTTGGATCACTC 5 0.125 No Hit CGTTGGTTTGTGAATAAGATCAACAGCTGTCTCCACCTTGTTGACATTTT 5 0.125 No Hit GCTTTCAATTCTAGAAAAAAAAAAACAAAAAAAAGCTGGATGTAATTTAC 5 0.125 No Hit AAAGGGTTGGGACTTCCCATGACTCCTTGTATATGGAAGCTCCAAGGCCT 5 0.125 No Hit GTGCTTTTTGCAGTACTCGATCACCTTAGCAAGAACCTTGCTTGTAACGT 5 0.125 No Hit CACGTGAGCTAGAAGAGGAAGAAGGTTTCGATGCCGAATGTGGCCAACGG 5 0.125 No Hit GGCAGCAAGAGCAGAGCCAGAAGAAGTTGCGGGTATCGAAGTAATGGCTG 5 0.125 No Hit ATCCACGTCTCACAAAACACGAGAAATTCTTGATAACACTGAAAGCATAG 5 0.125 No Hit GTTCTAGACAAAAACCATTGTTCAAGACTTTCTATGAGAGAACTACCCAC 5 0.125 No Hit GGCATTAGTCATCTGGGCTCTTCTTCCTGAAGCATGTGTAGTTCCTCCTC 5 0.125 No Hit GCATCTGAAACAACTTGTTGAGGGGACTACCAAAATGGAAATTCCCACCC 5 0.125 No Hit GCTGGGGCAGTTGATGTTGAAGATGGCGATGGTGTTGGTGATGGCTCGGC 5 0.125 No Hit GGCCTGCATAACTGCAATGTTCTGATTGGCGTCTTTTGTGAGGACATGAA 5 0.125 No Hit ATTAGTTCGGGCCCTAAGGTGAATATGATCCCTCAAAAACTCAAGGGAGA 5 0.125 No Hit GCCATCTCCAACTTGGCTGCAGATTGGAATGACAGCAGAGAAAGATCTCT 5 0.125 No Hit CCGTTGATCGGGAGTGCCTGAAATAAGAAGAGAGTAATCTATGACAGGAA 5 0.125 No Hit CTCCGCAAACATCATTTCCACTTCCTTGCCAACTTGGAGTACTTTTTCCA 5 0.125 No Hit CAACAAATATAAGATTGAACAAGCAGAATTTGACACGAGCCATACAAGGC 5 0.125 No Hit CTTCCGATCTTAGCCCACACGCTCTGATGGTAACCAGCAGTCACAGGAGC 5 0.125 No Hit GTCATAAGAGAGGGTCCGACAGCCATTGCTGCTTCAGTGGTTGGAACCAT 5 0.125 No Hit AGACAAAACAGAATTGACAAGCACTAATCATCATACAGTGAATAACTTAT 5 0.125 No Hit GCTTGTCGGAACCCAGCAGGTAAATCCATCACTGTTTTATGCCACTCATT 5 0.125 No Hit CTGGTCTTTGATACGATAACGGTGTAACCATCCTCGGCGCTAGGCACGAA 5 0.125 No Hit CACCAATTTTCCAATGTCTTCTGTTTCTCCCTTGAGCATTGTAGTTTGAT 5 0.125 No Hit CAGTAAACTATATTGAATGAAAATAACATCACAATAACAAACATTTTTTT 5 0.125 No Hit GTCCGATATGATACGCGAAGTGAGCCTCTAATGTTTTTTTCTTCCTGTCA 5 0.125 No Hit ATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAAT 5 0.125 No Hit CCCCCCCCTTCATTGCCTACATAGAACAACTGAATAGCATCTTCAAGCTT 5 0.125 No Hit AAAGGGAGGAGCTATGGATGCTGATTCATGGAGTGCTCGACTTTCCTCAG 5 0.125 No Hit GCATAAGGATTTGGACCTTGTTAAATTGGAACCACTCATAATAAGGAGGG 5 0.125 No Hit TTCGCCTCCTCGAGCTCAATCAGCACCTGAGATGCCTCAGTGCATCCAAA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.025 0.0 0.0 0.0 70-71 0.025 0.05 0.0 0.0 0.0 72-73 0.025 0.05 0.0 0.0 0.0 74-75 0.05 0.05 0.0 0.0 0.0 76-77 0.075 0.05 0.0 0.0 0.0 78-79 0.0875 0.05 0.0 0.0 0.0 80-81 0.1375 0.05 0.0 0.0 0.0 82-83 0.2 0.05 0.0 0.0 0.0 84-85 0.275 0.05 0.0 0.0 0.0 86-87 0.42500000000000004 0.05 0.0 0.0 0.0 88-89 0.55 0.05 0.0 0.0 0.0 90-91 0.675 0.05 0.0 0.0 0.0 92-93 0.7875 0.05 0.0 0.0 0.0 94-95 0.95 0.05 0.0 0.0 0.0 96-97 1.0375 0.05 0.0 0.0 0.0 98-99 1.2125 0.05 0.0 0.0 0.0 100-101 1.3250000000000002 0.05 0.0 0.0 0.0 102-103 1.5125 0.05 0.0 0.0 0.0 104-105 1.9249999999999998 0.05 0.0 0.0 0.0 106-107 2.075 0.05 0.0 0.0 0.0 108-109 2.325 0.05 0.0 0.0 0.0 110-111 2.7 0.05 0.0 0.0 0.0 112-113 3.05 0.05 0.0 0.0 0.0 114-115 3.3625 0.05 0.0 0.0 0.0 116-117 3.525 0.05 0.0 0.0 0.0 118-119 3.8125 0.05 0.0 0.0 0.0 120-121 4.375 0.05 0.0 0.0 0.0 122-123 4.725 0.05 0.0 0.0 0.0 124-125 5.1375 0.05 0.0 0.0 0.0 126-127 5.4125 0.05 0.0 0.0 0.0 128-129 5.9 0.05 0.0 0.0 0.0 130-131 6.65 0.05 0.0 0.0 0.0 132-133 7.2 0.05 0.0 0.0 0.0 134-135 7.7 0.05 0.0 0.0 0.0 136-137 8.6 0.05 0.0 0.0 0.0 138-139 9.05 0.05 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTGGTT 10 0.006830828 145.0 145 TTCCATT 10 0.006830828 145.0 2 TCGTCAT 10 0.006830828 145.0 9 AGCACAT 10 0.006830828 145.0 3 ATCGTCA 10 0.006830828 145.0 8 GCACATC 10 0.006830828 145.0 4 CATCGTC 10 0.006830828 145.0 7 >>END_MODULE SRR13695454 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695454_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.2025 37.0 37.0 37.0 37.0 37.0 2 36.2495 37.0 37.0 37.0 37.0 37.0 3 36.1775 37.0 37.0 37.0 37.0 37.0 4 36.1985 37.0 37.0 37.0 37.0 37.0 5 36.322 37.0 37.0 37.0 37.0 37.0 6 36.298 37.0 37.0 37.0 37.0 37.0 7 36.1795 37.0 37.0 37.0 37.0 37.0 8 36.275 37.0 37.0 37.0 37.0 37.0 9 36.2945 37.0 37.0 37.0 37.0 37.0 10-14 36.254200000000004 37.0 37.0 37.0 37.0 37.0 15-19 36.2417 37.0 37.0 37.0 37.0 37.0 20-24 36.27295 37.0 37.0 37.0 37.0 37.0 25-29 36.21355 37.0 37.0 37.0 37.0 37.0 30-34 36.193799999999996 37.0 37.0 37.0 37.0 37.0 35-39 36.14595 37.0 37.0 37.0 37.0 37.0 40-44 36.14455 37.0 37.0 37.0 37.0 37.0 45-49 36.1357 37.0 37.0 37.0 37.0 37.0 50-54 36.115300000000005 37.0 37.0 37.0 37.0 37.0 55-59 36.068149999999996 37.0 37.0 37.0 37.0 37.0 60-64 36.04644999999999 37.0 37.0 37.0 37.0 37.0 65-69 35.995999999999995 37.0 37.0 37.0 37.0 37.0 70-74 35.97135 37.0 37.0 37.0 37.0 37.0 75-79 36.017849999999996 37.0 37.0 37.0 37.0 37.0 80-84 35.991299999999995 37.0 37.0 37.0 37.0 37.0 85-89 35.859300000000005 37.0 37.0 37.0 37.0 37.0 90-94 35.798950000000005 37.0 37.0 37.0 37.0 37.0 95-99 35.84740000000001 37.0 37.0 37.0 37.0 37.0 100-104 35.82045 37.0 37.0 37.0 37.0 37.0 105-109 35.83540000000001 37.0 37.0 37.0 37.0 37.0 110-114 35.78665 37.0 37.0 37.0 37.0 37.0 115-119 35.76005 37.0 37.0 37.0 37.0 37.0 120-124 35.68365 37.0 37.0 37.0 37.0 37.0 125-129 35.5792 37.0 37.0 37.0 37.0 37.0 130-134 35.45855 37.0 37.0 37.0 37.0 37.0 135-139 35.4433 37.0 37.0 37.0 34.6 37.0 140-144 35.361749999999994 37.0 37.0 37.0 34.6 37.0 145-149 35.1382 37.0 37.0 37.0 27.4 37.0 150-151 34.94225 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 1.0 14 1.0 15 1.0 16 2.0 17 2.0 18 3.0 19 2.0 20 0.0 21 2.0 22 4.0 23 3.0 24 4.0 25 8.0 26 9.0 27 12.0 28 11.0 29 19.0 30 21.0 31 42.0 32 50.0 33 99.0 34 206.0 35 585.0 36 2683.0 37 229.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.95434019066733 21.525338685398896 14.325137982940293 27.19518314099348 2 29.099999999999998 26.825 27.700000000000003 16.375 3 20.674999999999997 27.925 31.275 20.125 4 21.825 33.575 24.95 19.650000000000002 5 26.700000000000003 36.175000000000004 20.925 16.2 6 20.825 41.55 19.675 17.95 7 20.8 23.425 37.5 18.275 8 21.425 24.975 29.049999999999997 24.55 9 20.724999999999998 26.125 31.474999999999998 21.675 10-14 22.465 30.665 26.945000000000004 19.925 15-19 22.82 28.46 28.405 20.315 20-24 22.736136806840342 28.59142957147857 28.171408570428518 20.501025051252565 25-29 22.48562140535134 27.746936734183546 28.587146786696678 21.180295073768445 30-34 22.127212721272127 29.07790779077908 27.93779377937794 20.857085708570857 35-39 22.561128056402822 28.68143407170359 28.471423571178562 20.286014300715035 40-44 22.333350002500374 28.31424713707056 28.55428314247137 20.798119717957693 45-49 22.397239723972397 27.867786778677868 28.38783878387839 21.34713471347135 50-54 22.175 28.51 27.79 21.525 55-59 22.34558639659915 28.107026756689173 28.532133033258315 21.015253813453363 60-64 22.24611230561528 29.066453322666135 27.896394819740987 20.7910395519776 65-69 22.845 28.525 27.76 20.87 70-74 22.77069267316829 28.35708927231808 27.866966741685424 21.005251312828207 75-79 22.378356753513025 28.809321398209732 27.649147372105816 21.163174476171427 80-84 22.725 28.055000000000003 28.389999999999997 20.830000000000002 85-89 23.16963392678536 28.425685137027408 27.825565113022606 20.579115823164635 90-94 22.48612430621531 29.016450822541128 27.611380569028455 20.88604430221511 95-99 23.995 28.410000000000004 27.334999999999997 20.26 100-104 22.91072768192048 29.077269317329336 27.33183295823956 20.68017004251063 105-109 23.314662932586515 27.960592118423683 27.940588117623527 20.784156831366275 110-114 23.85619280964048 28.836441822091103 27.03635181759088 20.271013550677534 115-119 23.528529279391908 27.57913687053058 27.669150372555883 21.22318347752163 120-124 23.975993998499625 27.731932983245812 27.6419104776194 20.650162540635158 125-129 24.65993198639728 28.170634126825366 26.690338067613524 20.479095819163835 130-134 24.003201760968533 28.165491020061033 27.485116814247835 20.3461904047226 135-139 24.50480192076831 28.686474589835935 27.145858343337338 19.662865146058422 140-144 25.306265313265662 28.641432071603578 26.161308065403272 19.890994549727488 145-149 26.190476190476193 27.566026410564227 26.775710284113647 19.46778711484594 150-151 25.88838838838839 28.040540540540544 26.63913913913914 19.43193193193193 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.5 6 1.5 7 0.5 8 1.0 9 1.5 10 1.0 11 0.5 12 0.0 13 0.0 14 0.5 15 0.5 16 0.5 17 0.5 18 0.5 19 0.5 20 1.0 21 2.0 22 1.0 23 0.0 24 1.0 25 3.5 26 8.5 27 8.5 28 8.0 29 11.5 30 13.5 31 27.5 32 53.0 33 59.0 34 52.5 35 66.0 36 103.0 37 139.5 38 158.0 39 178.5 40 212.0 41 222.0 42 235.0 43 262.5 44 252.5 45 261.5 46 268.5 47 249.0 48 241.0 49 199.0 50 145.5 51 117.5 52 99.5 53 81.0 54 63.5 55 54.0 56 38.5 57 22.0 58 18.5 59 17.0 60 8.0 61 2.5 62 5.0 63 5.0 64 2.0 65 1.5 66 1.5 67 1.0 68 1.5 69 0.5 70 0.0 71 0.5 72 0.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35000000000000003 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.005 25-29 0.025 30-34 0.01 35-39 0.005 40-44 0.015 45-49 0.01 50-54 0.0 55-59 0.025 60-64 0.005 65-69 0.0 70-74 0.025 75-79 0.015 80-84 0.0 85-89 0.02 90-94 0.005 95-99 0.0 100-104 0.025 105-109 0.02 110-114 0.005 115-119 0.015 120-124 0.025 125-129 0.02 130-134 0.055 135-139 0.04 140-144 0.005 145-149 0.04 150-151 0.1 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.825 #Duplication Level Percentage of deduplicated Percentage of total 1 70.97711587359244 48.85 2 18.706865237922266 25.75 3 6.610969851071559 13.65 4 2.106792589901925 5.800000000000001 5 1.1260443152924082 3.875 6 0.32691609153650564 1.35 7 0.10897203051216856 0.525 8 0.03632401017072285 0.2 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGAGGAGTTTTGTGATTTCATGCAAGAGATGATCTCTATGATGAACAGTG 8 0.2 No Hit CCAGATACCACTGAGTGGTCCAAATGCAGTGATTGGAAGAGCACTTGTGG 7 0.17500000000000002 No Hit AAATAGTGCAGAATGATCACTACTGGCATGAAAAGAAAGACTTCGCATTA 7 0.17500000000000002 No Hit GGGAAAAGAATCTAGAATGCTGGTTTTGGTAAAAATGTTACTTGGTAAAG 7 0.17500000000000002 No Hit CTATAACCAACAACTGATAATTCGATCTCGTGGGTTGACATGTTACTGTA 6 0.15 No Hit CTTTAGTGATAGCTATTCTTATAGCTACCATTGCCTTCTCTCCCTTATCC 6 0.15 No Hit ACCACATATAGAGGGTGTAATAGCTAAGTAGCCTGTAAGAGATGGCTTCC 6 0.15 No Hit ATCAACTCCAATGATTTCTTCTCGGGCTTTCTCTCTAATGCGCAAGCCAT 6 0.15 No Hit AGAAAGGCTAATGACATTACTTCCATTGCAAGCAATGGTGGACGAGTTCA 6 0.15 No Hit ACTTGGCAAAGAATTTGGCCGGTGATGTTATTGGAGTCCGAGTAGATGCC 6 0.15 No Hit ATTATTCCCCTCCAAAAAGAAGGCATCCCTCAAGGTTTGCAATTATTACT 6 0.15 No Hit CGATGAGGAGAAGCAGACCGAAAAGGACAGATGGGGAGGCCTTGTTACAG 6 0.15 No Hit CAGCAATAAACACGGCAATGGCTGCTCTCACAAAAACAGTGGGTGTGTTT 6 0.15 No Hit AAGGGAGGCAGGCATGGGTGTAGAGGTCCTGGGACTATGGTTACCTAATC 5 0.125 No Hit GACCAACTTCCTGGTGGAAGTGTTTCATTGGGCGAAGCTCTAATGGCTCC 5 0.125 No Hit CTCTTTCATTCGCATGACCCTCTTCACTAGCTAGTTCTAACCATGGCTTC 5 0.125 No Hit CCAAGTTTTTTATATACATTCCCTAAAGAAGTTCCAGCCTTTTTCCTTTT 5 0.125 No Hit AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 5 0.125 No Hit CATGCAGGTGTGGCCTCCAACTGGATTGAAGAAGTTCGAGACTCTTTCTT 5 0.125 No Hit CAGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 5 0.125 No Hit ATGTGATGGAGGTCAAAGGAAAAAGTGTCTACAGTAAATTGAAATACGAG 5 0.125 No Hit GTTCGCAGGAAGCTCGATGCTGAGAATAGCAGTGCTCCCACTGATAGCTG 5 0.125 No Hit GAAACTGTAGCAACCGCTTCTTTTGCAGGAAAGTACATTATTGTGAAGGA 5 0.125 No Hit TAGAATATTTTAGGTGCATGACCTAACTAAGCCTTTCTTGTTTTTCAAAA 5 0.125 No Hit TCCCATTCTTGGATTCCATGGTATGCTCATCATCAATGTGACAACACAAC 5 0.125 No Hit AGCTAGTGCTAGGATTTCTTGCTCTTTGCAATCTGACCTTAAAGACTTGG 5 0.125 No Hit CAAAGGTTATGTTGATTATATGGGACACTGAAGTTGAATCGGATAATTTC 5 0.125 No Hit GGAGAAATTCTGAAGACACAAGTGCACAAGTGGCCTGAATCAGTGCTTCA 5 0.125 No Hit ACATGGTCAATAAAACTTTCTAACAGCAGACTGGCTTTTCCACTCTCTTT 5 0.125 No Hit GCACAAGCGTATCTAAAAAGGAGGAAGCCCTGAGTGTGCTCGGTGCAGAC 5 0.125 No Hit AGTGCCTGTTCCACCATGTCCATGCCCTCTTTACGCAGCAAGTTAAATCA 5 0.125 No Hit CCTATATACAGGGACACTGTACCTTTGCAAAGAGAAGGCGTTCTGGCTGT 5 0.125 No Hit ATGCTGGTGTAGCAGATCTGAGCACGCTTGTGCAGACTGGGACTTGCGTG 5 0.125 No Hit CTCGCATTCTCCTTCCTCCTGCCACGGTGCTACCTTCCCCCTCTCTAAAA 5 0.125 No Hit ATTCAAATGGTTTCCTGACTGACCTGTTCAGGATCTATCCGAGATGTTGA 5 0.125 No Hit TGAAACAGCTTCCCTTCTTCCCTCCCAAGCATTTCCTAGTCAACCTACCA 5 0.125 No Hit GGAGAAGTATTCAAAGCTGAATTGCCTGGAAGTAATGGAAAAATGATTGC 5 0.125 No Hit AGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATC 5 0.125 No Hit GTGGTGATGGAGGTGATGATGACGGTGATGATGATGATTACTTTGATGAC 5 0.125 No Hit GTGGTGGAGGGGTTGGATGTGTCCAGTCTAAAGGAAATATGTTAGGAAAC 5 0.125 No Hit GTACCGTTGGTTTAAATTTTTTCTTTATCCAGTTCTCTATTACAGGTTAG 5 0.125 No Hit GCTGAGGCCAAAGCTTGTGGTGAAAAGATTAGCACTAAGAGGCAGCCTCA 5 0.125 No Hit GCTAAAGCCTATTGCCACCGGGATGACTTCCTACATGGGAAGATTATTCG 5 0.125 No Hit TGTCGCTGTAGCTTCTATTTTCTCTCAATCAAAGAGCAAAATAATGTCGA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.0875 0.0 0.0 0.0 0.0 80-81 0.1375 0.0 0.0 0.0 0.0 82-83 0.2 0.0 0.0 0.0 0.0 84-85 0.275 0.0 0.0 0.0 0.0 86-87 0.44999999999999996 0.0 0.0 0.0 0.0 88-89 0.575 0.0 0.0 0.0 0.0 90-91 0.7125 0.0 0.0 0.0 0.0 92-93 0.8375 0.0 0.0 0.0 0.0 94-95 1.0 0.0 0.0 0.0 0.0 96-97 1.0875 0.0 0.0 0.0 0.0 98-99 1.2625000000000002 0.0 0.0 0.0 0.0 100-101 1.375 0.0 0.0 0.0 0.0 102-103 1.5625 0.0 0.0 0.0 0.0 104-105 1.975 0.0 0.0 0.0 0.0 106-107 2.125 0.0 0.0 0.0 0.0 108-109 2.375 0.0 0.0 0.0 0.0 110-111 2.75 0.0 0.0 0.0 0.0 112-113 3.1 0.0 0.0 0.0 0.0 114-115 3.425 0.0 0.0 0.0 0.0 116-117 3.6 0.0 0.0 0.0 0.0 118-119 3.8874999999999997 0.0 0.0 0.0 0.0 120-121 4.45 0.0 0.0 0.0 0.0 122-123 4.800000000000001 0.0 0.0 0.0 0.0 124-125 5.3375 0.0 0.0 0.0 0.0 126-127 5.6875 0.0 0.0 0.0 0.0 128-129 6.1875 0.0 0.0 0.0 0.0 130-131 6.949999999999999 0.0 0.0 0.0 0.0 132-133 7.5 0.0 0.0 0.0 0.0 134-135 7.9375 0.0 0.0 0.0 0.0 136-137 8.775 0.0 0.0 0.0 0.0 138-139 9.2 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTTTTC 10 0.006830828 145.0 1 TACATTT 10 0.006830828 145.0 3 ATAAAAG 10 0.006830828 145.0 145 >>END_MODULE Read 1045762 spots for SRR13695454.sra Written 1045762 spots for SRR13695454.sra Read 1045762 spots for SRR13695454.sra Written 1045762 spots for SRR13695454.sra Read 1045762 spots for SRR13695454.sra Written 1045762 spots for SRR13695454.sra Read 1045762 spots for SRR13695454.sra Written 1045762 spots for SRR13695454.sra Read 1045762 spots for SRR13695454.sra Written 1045762 spots for SRR13695454.sra Read 1045762 spots for SRR13695454.sra Written 1045762 spots for SRR13695454.sra Read 1045762 spots for SRR13695454.sra Written 1045762 spots for SRR13695454.sra Read 1045762 spots for SRR13695454.sra Written 1045762 spots for SRR13695454.sra Read 1045762 spots for SRR13695454.sra Written 1045762 spots for SRR13695454.sra Read 1045762 spots for SRR13695454.sra Written 1045762 spots for SRR13695454.sra Read 1045762 spots for SRR13695454.sra Written 1045762 spots for SRR13695454.sra Read 1045762 spots for SRR13695454.sra Written 1045762 spots for SRR13695454.sra Read 1045762 spots for SRR13695454.sra Written 1045762 spots for SRR13695454.sra Read 1045762 spots for SRR13695454.sra Written 1045762 spots for SRR13695454.sra Read 1045762 spots for SRR13695454.sra Written 1045762 spots for SRR13695454.sra Read 1045762 spots for SRR13695454.sra Written 1045762 spots for SRR13695454.sra Read 1045764 spots for SRR13695454.sra Written 1045764 spots for SRR13695454.sra Read 1045762 spots for SRR13695454.sra Written 1045762 spots for SRR13695454.sra Read 1045762 spots for SRR13695454.sra Written 1045762 spots for SRR13695454.sra Read 1045762 spots for SRR13695454.sra Written 1045762 spots for SRR13695454.sra SRR ids: ['SRR13695454.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_upbolkoa SRR13695454.sra spots: 20915242 blocks: [[1, 1045762], [1045763, 2091524], [2091525, 3137286], [3137287, 4183048], [4183049, 5228810], [5228811, 6274572], [6274573, 7320334], [7320335, 8366096], [8366097, 9411858], [9411859, 10457620], [10457621, 11503382], [11503383, 12549144], [12549145, 13594906], [13594907, 14640668], [14640669, 15686430], [15686431, 16732192], [16732193, 17777954], [17777955, 18823716], [18823717, 19869478], [19869479, 20915242]] SRR13695454 file size 7086213 SRR13695454 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695454 SRR13695454_1.fastq SRR13695454_2.fastq Input file: SRR13695454_1.fastq Paired file: SRR13695454_2.fastq trimmed: SRR13695454-trimmed-pair1.fastq, SRR13695454-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 03:51:36 2025 >> started Wed Feb 12 03:51:59 2025 >> done (23.018s) 20915242 read pairs processed; of these: 116 ( 0.00%) short read pairs filtered out after trimming by size control 1563 ( 0.01%) empty read pairs filtered out after trimming by size control 20913563 (99.99%) read pairs available; of these: 2398552 (11.47%) trimmed read pairs available after processing 18515011 (88.53%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 2 0.00% 20 3 0.00% 21 2 0.00% 22 3 0.00% 23 2 0.00% 24 1 0.00% 25 3 0.00% 26 3 0.00% 27 5 0.00% 28 3 0.00% 29 3 0.00% 30 3 0.00% 31 4 0.00% 32 8 0.00% 33 4 0.00% 34 6 0.00% 35 7 0.00% 36 3 0.00% 37 5 0.00% 38 12 0.00% 39 9 0.00% 40 7 0.00% 41 21 0.00% 42 14 0.00% 43 23 0.00% 44 23 0.00% 45 26 0.00% 46 24 0.00% 47 33 0.00% 48 46 0.00% 49 49 0.00% 50 49 0.00% 51 77 0.00% 52 101 0.00% 53 96 0.00% 54 130 0.00% 55 120 0.00% 56 134 0.00% 57 186 0.00% 58 184 0.00% 59 288 0.00% 60 277 0.00% 61 352 0.00% 62 425 0.00% 63 482 0.00% 64 461 0.00% 65 557 0.00% 66 690 0.00% 67 716 0.00% 68 893 0.00% 69 1025 0.00% 70 1206 0.01% 71 1352 0.01% 72 1686 0.01% 73 1733 0.01% 74 2175 0.01% 75 2179 0.01% 76 2451 0.01% 77 2735 0.01% 78 3097 0.01% 79 3468 0.02% 80 3818 0.02% 81 4420 0.02% 82 5089 0.02% 83 5477 0.03% 84 6237 0.03% 85 6894 0.03% 86 7136 0.03% 87 7892 0.04% 88 8271 0.04% 89 9058 0.04% 90 9847 0.05% 91 10452 0.05% 92 11285 0.05% 93 12264 0.06% 94 13156 0.06% 95 14195 0.07% 96 14991 0.07% 97 15755 0.08% 98 16407 0.08% 99 17077 0.08% 100 18131 0.09% 101 18543 0.09% 102 19798 0.09% 103 20977 0.10% 104 22034 0.11% 105 22499 0.11% 106 23734 0.11% 107 24771 0.12% 108 25304 0.12% 109 26424 0.13% 110 26888 0.13% 111 28444 0.14% 112 29402 0.14% 113 29862 0.14% 114 30636 0.15% 115 32074 0.15% 116 33864 0.16% 117 34834 0.17% 118 35720 0.17% 119 36255 0.17% 120 37820 0.18% 121 38762 0.19% 122 38869 0.19% 123 40765 0.19% 124 41761 0.20% 125 42730 0.20% 126 44037 0.21% 127 44795 0.21% 128 45660 0.22% 129 47398 0.23% 130 48300 0.23% 131 48557 0.23% 132 49609 0.24% 133 50758 0.24% 134 51790 0.25% 135 52688 0.25% 136 53529 0.26% 137 54591 0.26% 138 55711 0.27% 139 57360 0.27% 140 57563 0.28% 141 58478 0.28% 142 59616 0.29% 143 60112 0.29% 144 61214 0.29% 145 62132 0.30% 146 62570 0.30% 147 64272 0.31% 148 64997 0.31% 149 65646 0.31% 150 66865 0.32% 151 18515011 88.53% 20913563 reads passed initial QC criterion=sequence-density sequence-density=0.21 sequence-density-rank=1 fanout-score=1.72 fanout-score-rank=40 prefix-density=0.35 prefix-fanout=1.0 sequence=TTGTCGAATCCGATTATACGGATAAAGGCGTTAGGGTAAGCTTTCTTTGCCTCCTCAAGCTCAAGCAACACTTGAGATGCCTCAGTGCATCCAAACATGGGTAGCTTCCACATAGTCCAGTAGCGTCCATCATAGTACCCTGGTGAGCTGTGGTGCTCACGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTTCGAGCGAAGAAGGTAATCAATTTCCTTGGCCAATTCCTCCTCTGTGAGATCTGGAAGGTAAGAAAGAGTCTCGAACTTCTTCAATCCAGTTGGTGGCCACACCTGCATGCATTGAACTCGTCCACCATTGCTTGCAATGGAAGTAATGTCATTAGCCTTTCTGGTACTGACTGGGAAAGCTGCGGCAGACTTGAGACCATTGAATGGTGCCACCATGTTGGCTTGTGCCGGGGTGCGGTTGACGGTGGCAACGGCTGCCGATGAGATCATAGAGGAGGAAGCCAT criterion=fanout-score sequence-density=0.01 sequence-density-rank=39 fanout-score=87.14 fanout-score-rank=1 prefix-density=0.07 prefix-fanout=8.2 sequence=CAAGCTTCCGATTAAAGATACATAATTCCATGGAATGGAACCAACAAAGCAGCAGGAAATACAAGACACTTACAGATTACTAGCCATCAAATGAGATCCTGTAGAAAGGATTTGAGGAGGCCATGGCTAGCTAACTGTACTTTAATTTACAGCAAATACTATATTAGACAAACATGGAGTGACCAGACTTCTTCTTTTTATTTATTATAGTTCCATAAAACTGCTTGGTTGGAGCCATGCGGCGACGTTTTCTCATTTGCAGGAGCATGGATCACAGGTGCAGTTTGATCCACATTTGCAGCCATTCTCAGCACCAAAGTTCATCTCAGAGCTCTCGTAGAACATCCTAACTGGAGCTACACCAGCAATGATTGTCTGACTTGTGGTGGTCTCGGAGAAACTCAAGTCTGGGTACATGCTGCATCCATTGCAGCCACTGCCGCACTTGCA criterion=sequence-density sequence-density=0.54 sequence-density-rank=1 fanout-score=2.05 fanout-score-rank=34 prefix-density=0.55 prefix-fanout=2.0 sequence=CTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGA criterion=fanout-score sequence-density=0.14 sequence-density-rank=13 fanout-score=29.83 fanout-score-rank=1 prefix-density=0.37 prefix-fanout=11.4 sequence=AAAGAAAAGAAAA SRR13695454 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 03:52:40 Started mapping on | Feb 12 03:52:40 Finished on | Feb 12 03:54:45 Mapping speed, Million of reads per hour | 602.31 Number of input reads | 20913563 Average input read length | 296 UNIQUE READS: Uniquely mapped reads number | 19750618 Uniquely mapped reads % | 94.44% Average mapped length | 294.94 Number of splices: Total | 19348876 Number of splices: Annotated (sjdb) | 18928075 Number of splices: GT/AG | 18962999 Number of splices: GC/AG | 313355 Number of splices: AT/AC | 11783 Number of splices: Non-canonical | 60739 Mismatch rate per base, % | 0.37% Deletion rate per base | 0.03% Deletion average length | 2.87 Insertion rate per base | 0.02% Insertion average length | 2.34 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 494499 % of reads mapped to multiple loci | 2.36% Number of reads mapped to too many loci | 77473 % of reads mapped to too many loci | 0.37% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.68% % of reads unmapped: other | 0.14% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 668699 668699 668699 N_multimapping 494499 494499 494499 N_noFeature 792826 19283136 1106303 N_ambiguous 276388 2069 120996 UnstrandedReadsAssigned:18681404 PositiveStrandReadsAssigned:465413 NegativeStrandReadsAssigned:18523319 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695454 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695454-trimmed-pair1.fastq SRR13695454-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 20,913,563 reads, 18,547,209 reads pseudoaligned [quant] estimated average fragment length: 251.363 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,086 rounds 52401 SRR13695454.ke.tsv 34699 SRR13695454.se.tsv 87100 total ==> SRR13695454.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1767.64 833 24.6169 Potri.005G024800.1.v4.1 1035 784.637 347 23.1016 Potri.004G059700.1.v4.1 961 710.759 10 0.734952 Potri.007G009000.2.v4.1 1416 1165.64 0 0 Potri.003G141000.2.v4.1 2943 2692.64 1035 20.0791 Potri.016G087400.1.v4.1 270 85.24 1280 784.418 Potri.015G069301.1.v4.1 564 323.764 0 0 Potri.010G195200.1.v4.1 1773 1522.64 69 2.36719 Potri.012G127500.1.v4.1 977 726.71 52 3.73786 ==> SRR13695454.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 301 Potri.001G233950.v4.1 1 Potri.001G122700.v4.1 295 Potri.001G212900.v4.1 2 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 18 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 6 SRR13695454 completed mapping pipeline successfully