Starting /dee2/code/volunteer_pipeline.sh SRR13695455 current disk space = 3049188376576 free memory = 1510805672 SRR13695455 SRAfilesize 8bc164455b7389e5ca8ba645c2c12920 SRR13695455.sra SRR13695455.sra file validated SRR13695455 is paired end SRR13695455 is conventional basespace SRR13695455 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695455_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.563 37.0 37.0 37.0 37.0 37.0 2 36.342 37.0 37.0 37.0 37.0 37.0 3 36.5665 37.0 37.0 37.0 37.0 37.0 4 36.611 37.0 37.0 37.0 37.0 37.0 5 36.567 37.0 37.0 37.0 37.0 37.0 6 36.536 37.0 37.0 37.0 37.0 37.0 7 36.435 37.0 37.0 37.0 37.0 37.0 8 36.5305 37.0 37.0 37.0 37.0 37.0 9 36.5445 37.0 37.0 37.0 37.0 37.0 10-14 36.56080000000001 37.0 37.0 37.0 37.0 37.0 15-19 36.4818 37.0 37.0 37.0 37.0 37.0 20-24 36.4736 37.0 37.0 37.0 37.0 37.0 25-29 36.454 37.0 37.0 37.0 37.0 37.0 30-34 36.4356 37.0 37.0 37.0 37.0 37.0 35-39 36.437 37.0 37.0 37.0 37.0 37.0 40-44 36.39730000000001 37.0 37.0 37.0 37.0 37.0 45-49 36.3486 37.0 37.0 37.0 37.0 37.0 50-54 36.3663 37.0 37.0 37.0 37.0 37.0 55-59 36.4076 37.0 37.0 37.0 37.0 37.0 60-64 36.3721 37.0 37.0 37.0 37.0 37.0 65-69 36.3784 37.0 37.0 37.0 37.0 37.0 70-74 36.30210000000001 37.0 37.0 37.0 37.0 37.0 75-79 36.2862 37.0 37.0 37.0 37.0 37.0 80-84 36.261199999999995 37.0 37.0 37.0 37.0 37.0 85-89 36.2234 37.0 37.0 37.0 37.0 37.0 90-94 36.1828 37.0 37.0 37.0 37.0 37.0 95-99 36.1633 37.0 37.0 37.0 37.0 37.0 100-104 36.0612 37.0 37.0 37.0 37.0 37.0 105-109 36.092999999999996 37.0 37.0 37.0 37.0 37.0 110-114 36.0969 37.0 37.0 37.0 37.0 37.0 115-119 36.0226 37.0 37.0 37.0 37.0 37.0 120-124 35.9818 37.0 37.0 37.0 37.0 37.0 125-129 35.9841 37.0 37.0 37.0 37.0 37.0 130-134 36.0469 37.0 37.0 37.0 37.0 37.0 135-139 35.9368 37.0 37.0 37.0 37.0 37.0 140-144 35.8524 37.0 37.0 37.0 37.0 37.0 145-149 35.6356 37.0 37.0 37.0 37.0 37.0 150-151 35.565 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 2.0 25 5.0 26 8.0 27 7.0 28 14.0 29 20.0 30 28.0 31 35.0 32 48.0 33 75.0 34 119.0 35 300.0 36 2990.0 37 349.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.775 12.825000000000001 7.5249999999999995 43.875 2 19.814163736815672 13.58613761928679 37.89552988448016 28.704168759417374 3 17.125 18.15 26.525 38.2 4 22.575 24.05 23.7 29.675 5 24.625 31.374999999999996 23.325000000000003 20.674999999999997 6 21.95 33.15 23.549999999999997 21.349999999999998 7 16.55 27.325 40.825 15.299999999999999 8 17.5 28.225 33.1 21.175 9 16.650000000000002 24.275 33.925 25.15 10-14 20.36 29.445 26.755000000000003 23.44 15-19 20.095 28.73 27.465 23.71 20-24 20.025000000000002 28.360000000000003 28.1 23.515 25-29 19.939999999999998 28.62 27.265 24.175 30-34 19.615 28.355000000000004 28.04 23.990000000000002 35-39 19.855 28.544999999999998 27.634999999999998 23.965 40-44 20.244999999999997 28.015 27.825 23.915 45-49 20.275000000000002 28.044999999999998 27.665 24.015 50-54 20.47 28.125 27.400000000000002 24.005000000000003 55-59 20.46 27.395000000000003 27.860000000000003 24.285 60-64 20.455000000000002 27.845 28.4 23.3 65-69 20.575 28.7 26.71 24.015 70-74 20.39 28.249999999999996 27.139999999999997 24.22 75-79 20.49 27.99 28.105000000000004 23.415 80-84 20.355 28.615000000000002 27.525 23.505000000000003 85-89 20.925 28.89 26.8 23.385 90-94 20.86 27.615000000000002 27.63 23.895 95-99 20.96 27.355 28.29 23.395 100-104 20.14 27.73 28.470000000000002 23.66 105-109 21.52 28.105000000000004 26.91 23.465 110-114 20.849999999999998 28.285 27.765 23.1 115-119 21.18 28.605000000000004 27.105 23.11 120-124 20.71 28.1 27.565 23.625 125-129 20.979999999999997 27.48 27.88 23.66 130-134 21.665 27.975 26.674999999999997 23.685000000000002 135-139 20.575 29.310000000000002 26.46 23.655 140-144 21.875 28.849999999999998 25.495 23.78 145-149 20.325 28.035 26.71 24.93 150-151 21.6625 27.787499999999998 26.5125 24.0375 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.0 16 0.0 17 0.5 18 0.5 19 0.0 20 1.0 21 1.5 22 1.5 23 2.0 24 1.5 25 1.5 26 2.0 27 6.5 28 9.5 29 8.5 30 17.5 31 25.0 32 30.5 33 36.5 34 54.0 35 75.5 36 88.0 37 110.5 38 133.5 39 166.5 40 191.5 41 204.0 42 222.5 43 241.5 44 254.0 45 258.5 46 258.5 47 229.5 48 209.0 49 202.0 50 177.5 51 149.0 52 131.0 53 106.5 54 75.5 55 62.5 56 61.5 57 64.0 58 41.5 59 22.0 60 23.5 61 12.0 62 8.5 63 8.5 64 3.5 65 3.0 66 0.0 67 0.5 68 0.5 69 1.5 70 1.5 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.44999999999999996 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.8 #Duplication Level Percentage of deduplicated Percentage of total 1 69.53592814371258 46.45 2 19.16167664670659 25.6 3 6.736526946107785 13.5 4 2.245508982035928 6.0 5 1.5718562874251496 5.25 6 0.6362275449101796 2.55 7 0.037425149700598806 0.17500000000000002 8 0.037425149700598806 0.2 9 0.0 0.0 >10 0.037425149700598806 0.27499999999999997 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGAGTTGGCGGCGAGGGAAGAGGAGGAAGTGTTTGGTGGGGGAGTGTG 11 0.27499999999999997 No Hit AGTGACCACTCCACCCACTCTTCCTTCTTTCACTATCAAGTCCTCTGCAG 8 0.2 No Hit GTAGAAATGTATGGAGGGCTTATGAGGTTATGAACTCGAAGAATACCCAT 7 0.17500000000000002 No Hit CACCTGGGAGCGAGCCATCGAGCCACTCGGGGTCAACCAAGCTGCCACCA 6 0.15 No Hit ATCGGATGAACAGCGTGAGCTCTTCTGATTGTTGAAGCAGAGGCCTCAGA 6 0.15 No Hit CTGCTATTCTAATAAGCTGGTCCAGTGCTACTCTCTGGTATGCTTCTTCT 6 0.15 No Hit GTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCAC 6 0.15 No Hit GTGAGAATATCTTTACTCCCCCAACCCACGAAGAGAAAAAAGCAATATGA 6 0.15 No Hit TAGGAGGAATAACTGTAGCAGTTGCCACGCCACCTCCTTCCTCTCCCTCC 6 0.15 No Hit GCAACATCATATATGGGACTCCACTGATTTTGTAAAATGTCTAAGCATAT 6 0.15 No Hit ATCGTGATTTGCAGCATCTCCTCCATCATCTGAAGAAACGTCACTGTTAG 6 0.15 No Hit GCCATCATCAAGTTCAGACCCCAAAAACCCATCACTTTCTTCACTAAAAA 6 0.15 No Hit GCCTGAATTGCAGCATTTGTCATTCACTAGTATATGAATTGTTCAGAACA 6 0.15 No Hit ATCCTGAACTGTCTCTGGTCCTTCTATAATGCAAAAATTGCTGGAATAGT 6 0.15 No Hit CGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCA 6 0.15 No Hit GGCTGTTTGAAACCTTATGCTTCTCCATTGCTTTATTTTCTCCTACCCAC 6 0.15 No Hit AGACCGGATTCTACTGATTCTTTAAGGCTAATGATTACATTAACACCACT 6 0.15 No Hit CTTCATTAAAACCACACCAGAGGCCACAGACATGGCCAATACATAACAAT 6 0.15 No Hit TCTGCAGCGGCACCACCGGCAGGACCGCCGCCGATGACGGCTACTCGGAG 6 0.15 No Hit CTCAGCAGCCTTAATGAAGACTTCCATTACTTTACTTGAGGCCAAAGACG 6 0.15 No Hit TACAGGTATCAGGAAAGCAGCTAAGCCAATGGCCTCTATGATCTTCAACA 5 0.125 No Hit CCTACGATTTGTGGTCTTGATAGCCGTATCAAGCGTCATAAATGATGTCT 5 0.125 No Hit CTTTATACAAACTCCAGGCTGTTTGGAGGGTGGTGAATGGTGAAAGGCTC 5 0.125 No Hit CGGCCATCCTCAAGCTGCTTACCGGCAAAGATCAACCTCTGCTGGTCTGG 5 0.125 No Hit AGGCTGGTTCCTTTGCGCCTCCTCAAAAAGAAGTTTCAATTGCCTTTCAG 5 0.125 No Hit CTTTGATCTACCACTATCCCTGTCATAAACCACTTTAGCATCCATCACCT 5 0.125 No Hit CCCCAAATGTTCTGGCCAAACCAAAATCTGAAATTTTCGGGTTCATACCA 5 0.125 No Hit CAGCATCATCGCCCCATATGAACTTCATCCTGCCCATTGCATAAGGTTGG 5 0.125 No Hit TAAAATTCATGAAACCTTCATGGTGATTGTTGTGATGAGCACACTTTGGA 5 0.125 No Hit CTCGTACTCCATGACATTGGTTATCTCCATCTTTAAAATCAACGGTTAAA 5 0.125 No Hit TGCGGCTTTTGATGTGGAATTGAGTTTCTTGAGCTGTGAAGTGGAGTTTA 5 0.125 No Hit GTCTTTGGCTTCCCGAAAATATTGGCAGATTCACCATAGGCAGCATCAGC 5 0.125 No Hit CCCCGTTGCCGGCTAAAAATGTCACGTAGGCCCTCTTAGAATAGCCAGTG 5 0.125 No Hit CCCATGTACAAACCCGTCCATCCCTTCCTACCACGGCAGCATGCCATGCA 5 0.125 No Hit CCCAGATGGAATGTGTCTATTAGTCTTGACATCCATTACAGATGTGAACA 5 0.125 No Hit CATCATTTCATTATCTGTGTTTCCCATCAAAGATTGAACTGTATCAATCA 5 0.125 No Hit AGATCGTTGAAGCCATCCTCCTGACTAGGAGCAGAAATTGAATGATTAGC 5 0.125 No Hit GGGTACTTGGGTCCAACTCCGGTGGAGTAAATCCCACTGGAGCCTCTTTT 5 0.125 No Hit CAGCAGTGGGGAGTGAAAGAGCACCCAAAAGAAGCAGATTCATAAGCTCC 5 0.125 No Hit CCCCCCTTCAACCTTGCCTCACGCTTCCATTGTACAAGTTGCTTTTCCTG 5 0.125 No Hit ATTGAACATAAGCGTAAAGAAAAGAACAGTGACTTTGCCTCTTGGATGCC 5 0.125 No Hit CCCCAGTTGAAGCCAATTGTCTGTTTCACCATGTTTTGCTCTTTGCGTCT 5 0.125 No Hit CCCAGCTTTTGTTACCGTCAGCGAGTCCTATTGTCTGCACAACAGAACCG 5 0.125 No Hit CCGATACATGTAAAATATCCACTGCTGAAATCACAAGTACCGGTGGAGTC 5 0.125 No Hit AGAGCTCACTCCAGCACAAGCACCAGCAATTAATGATGCAGGAATTGGAA 5 0.125 No Hit CCATGCTACTCGGGTCAGAGCGTAAGCTAGTGATGCTTTCGGCTACTGGA 5 0.125 No Hit CTTCCGGATCAGACTCACTGCTAATTCCTGCAATAAAACTTGGTAAAGCA 5 0.125 No Hit CCCGTACAACATTAAATAAGTACACACAGAGGAAGAGTAATTATTTCGAA 5 0.125 No Hit ATTGATTTTAGATGGGATTAATCTTATCAACTCTATGAACAGTTGAATAT 5 0.125 No Hit GCTCTTGATACGCTTTGTACTCCAGAGTTGTAGCCTGTTTCAAAGCAACT 5 0.125 No Hit GCGAGTACAACACCAAATATAAATATAACAAGGAAATGTGAGTTCCTTAG 5 0.125 No Hit GTTCTTGTTACCCTTCACACTAACATTAACGTTACTGTTATGGATATTAT 5 0.125 No Hit CTTTGGGACCGAGGGAGGTTCGAAGGATGCGAGAGACCGCCATACCAGAA 5 0.125 No Hit GGCCTAAGAAACATCCCACCAGATTCTCCAGTCCACTGTACTCCAGCCTT 5 0.125 No Hit CGTGGCTGCAGAATTAAGTCCTTTCAACCCACTGCAGCAAGCTGCAGGCA 5 0.125 No Hit CACCTTGTATAGTTCATTTGCAGCTGCTGCAATTGGGGTGGGTTGGGAAA 5 0.125 No Hit CTTGTTTTGTGGAATCTTCCTCTGCATTTCCTCCTGCGCGGTCCAGACAG 5 0.125 No Hit GCACACAAAAGGTAGTTCATCAGGAATACAAAACAAGTAACAAAAGCCCA 5 0.125 No Hit CCCTTTGACTCAGCTCTAGCAACTCATCGAAACTCTTGCACTTGGTTAAC 5 0.125 No Hit CTTTCTCAGTACAGCGTTAGCACTGTCTGCATCGTCATCAAGAACTTGAA 5 0.125 No Hit TATTGAACAAGTTACAGGTCCAAAGTTGACATCAGCTGAATACAACTGAT 5 0.125 No Hit CCAGGAATTCCAAATTTCAATCCCCCTACTTAAACCTAAAACCACACAAT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.1375 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.325 0.0 0.0 0.0 0.0 84-85 0.3375 0.0 0.0 0.0 0.0 86-87 0.375 0.0 0.0 0.0 0.0 88-89 0.4125 0.0 0.0 0.0 0.0 90-91 0.48750000000000004 0.0 0.0 0.0 0.0 92-93 0.6499999999999999 0.0 0.0 0.0 0.0 94-95 0.775 0.0 0.0 0.0 0.0 96-97 0.875 0.0 0.0 0.0 0.0 98-99 1.1 0.0 0.0 0.0 0.0 100-101 1.2125 0.0 0.0 0.0 0.0 102-103 1.35 0.0 0.0 0.0 0.0 104-105 1.525 0.0 0.0 0.0 0.0 106-107 1.725 0.0 0.0 0.0 0.0 108-109 2.1125 0.0 0.0 0.0 0.0 110-111 2.45 0.0 0.0 0.0 0.0 112-113 2.7875 0.0 0.0 0.0 0.0 114-115 3.15 0.0 0.0 0.0 0.0 116-117 3.45 0.0 0.0 0.0 0.0 118-119 3.8875 0.0 0.0 0.0 0.0 120-121 4.5375 0.0 0.0 0.0 0.0 122-123 5.0625 0.0 0.0 0.0 0.0 124-125 5.637499999999999 0.0 0.0 0.0 0.0 126-127 6.225 0.0 0.0 0.0 0.0 128-129 6.8875 0.0 0.0 0.0 0.0 130-131 7.425 0.0 0.0 0.0 0.0 132-133 7.8125 0.0 0.0 0.0 0.0 134-135 8.4375 0.0 0.0 0.0 0.0 136-137 9.325 0.0 0.0 0.0 0.0 138-139 10.037500000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGGGTT 10 0.006830828 145.0 1 TTCCATG 10 0.006830828 145.0 6 >>END_MODULE SRR13695455 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695455_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.13125 37.0 37.0 37.0 37.0 37.0 2 36.215 37.0 37.0 37.0 37.0 37.0 3 36.1965 37.0 37.0 37.0 37.0 37.0 4 36.228 37.0 37.0 37.0 37.0 37.0 5 36.2685 37.0 37.0 37.0 37.0 37.0 6 36.1695 37.0 37.0 37.0 37.0 37.0 7 36.266 37.0 37.0 37.0 37.0 37.0 8 36.259 37.0 37.0 37.0 37.0 37.0 9 36.264 37.0 37.0 37.0 37.0 37.0 10-14 36.2478 37.0 37.0 37.0 37.0 37.0 15-19 36.2315 37.0 37.0 37.0 37.0 37.0 20-24 36.203250000000004 37.0 37.0 37.0 37.0 37.0 25-29 36.15755 37.0 37.0 37.0 37.0 37.0 30-34 36.099199999999996 37.0 37.0 37.0 37.0 37.0 35-39 36.121449999999996 37.0 37.0 37.0 37.0 37.0 40-44 36.12205 37.0 37.0 37.0 37.0 37.0 45-49 36.11215 37.0 37.0 37.0 37.0 37.0 50-54 36.03155 37.0 37.0 37.0 37.0 37.0 55-59 35.98565000000001 37.0 37.0 37.0 37.0 37.0 60-64 35.959950000000006 37.0 37.0 37.0 37.0 37.0 65-69 35.9075 37.0 37.0 37.0 37.0 37.0 70-74 35.92145000000001 37.0 37.0 37.0 37.0 37.0 75-79 35.918850000000006 37.0 37.0 37.0 37.0 37.0 80-84 35.92555 37.0 37.0 37.0 37.0 37.0 85-89 35.78485 37.0 37.0 37.0 37.0 37.0 90-94 35.77795 37.0 37.0 37.0 37.0 37.0 95-99 35.74245 37.0 37.0 37.0 37.0 37.0 100-104 35.75855 37.0 37.0 37.0 37.0 37.0 105-109 35.7471 37.0 37.0 37.0 37.0 37.0 110-114 35.62865 37.0 37.0 37.0 37.0 37.0 115-119 35.65335 37.0 37.0 37.0 37.0 37.0 120-124 35.619749999999996 37.0 37.0 37.0 37.0 37.0 125-129 35.626250000000006 37.0 37.0 37.0 37.0 37.0 130-134 35.45415 37.0 37.0 37.0 37.0 37.0 135-139 35.500550000000004 37.0 37.0 37.0 37.0 37.0 140-144 35.384750000000004 37.0 37.0 37.0 34.6 37.0 145-149 35.26155 37.0 37.0 37.0 29.8 37.0 150-151 34.993750000000006 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 2.0 14 0.0 15 3.0 16 1.0 17 1.0 18 1.0 19 1.0 20 2.0 21 1.0 22 2.0 23 1.0 24 4.0 25 12.0 26 7.0 27 6.0 28 21.0 29 21.0 30 28.0 31 38.0 32 57.0 33 132.0 34 208.0 35 586.0 36 2658.0 37 206.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.07696164452244 22.93807971922788 10.930057658561044 29.054900977688643 2 27.900000000000002 23.7 31.674999999999997 16.725 3 19.825 26.700000000000003 33.975 19.5 4 23.7 33.275 25.3 17.724999999999998 5 25.074999999999996 36.575 20.875 17.474999999999998 6 20.875 39.625 22.875 16.625 7 20.125 22.125 40.1 17.65 8 21.675 24.95 28.4 24.975 9 21.349999999999998 25.5 31.0 22.15 10-14 22.835 29.25 26.44 21.475 15-19 23.119999999999997 28.42 27.139999999999997 21.32 20-24 22.998049317261042 28.765067773720805 27.699694893212623 20.537188015805533 25-29 22.61195896922692 28.536402301726294 27.845884413309985 21.0057543157368 30-34 23.011505752876438 28.16408204102051 28.554277138569283 20.27013506753377 35-39 22.86300205071775 28.044815685489922 28.129845445906064 20.96233681788626 40-44 22.49237080394217 28.375606583620993 27.975386462554404 21.156636149882434 45-49 21.93987294282427 29.033064879195635 28.63788704917213 20.389175128807963 50-54 23.103086080128044 28.3149102185765 27.81473515730506 20.767268543990397 55-59 23.062296722541905 27.705779334500875 28.066049537152864 21.165874405804352 60-64 22.592907517631172 27.744710648727057 28.735057270044518 20.927324563597256 65-69 22.979595919183836 27.500500100020002 28.160632126425284 21.359271854370874 70-74 23.112334250688015 28.136102076557417 27.220415311483613 21.53114836127095 75-79 22.917604682575416 27.675221371754468 28.635749662314275 20.771424283355845 80-84 23.090772693173292 28.497124281070267 26.911727931983 21.500375093773442 85-89 23.120028018211837 27.898133786961527 27.432831340371237 21.549006854455396 90-94 24.333516730855802 27.40959335767519 26.95443405191817 21.302455859550843 95-99 23.550887721930483 27.56689172293073 27.901975493873472 20.980245061265315 100-104 22.887165374030523 26.960220165123843 28.441330998248688 21.71128346259695 105-109 23.23626538577004 28.43490443310317 28.129690783548483 20.199139397578307 110-114 24.043415195318364 28.6950432651428 26.969439303756314 20.292102235782526 115-119 24.661029669285035 28.118276879971983 26.54225246410167 20.678440986641316 120-124 24.213159869902427 27.920940705529144 26.49987490617963 21.36602451838879 125-129 23.090008505528594 28.36343623355181 27.11762645719718 21.428928803722417 130-134 25.69927445584188 27.405554165624217 27.060295221416062 19.83487615711784 135-139 25.41906429822367 27.235426569927444 26.810107580685518 20.535401551163375 140-144 24.908718051317962 28.264892712449356 26.729355274346023 20.09703396188666 145-149 24.878658994245683 27.820865649236925 27.72579434575932 19.57468101075807 150-151 25.83187390542907 28.04603452589442 26.53239929947461 19.5896922692019 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 1.0 15 1.0 16 0.0 17 0.0 18 0.0 19 0.5 20 1.0 21 0.5 22 0.0 23 1.0 24 4.0 25 5.0 26 5.0 27 8.0 28 12.0 29 12.5 30 12.0 31 20.5 32 28.0 33 37.5 34 48.0 35 71.5 36 97.5 37 124.5 38 148.5 39 177.0 40 204.5 41 237.5 42 265.0 43 252.0 44 239.0 45 240.0 46 251.5 47 256.0 48 224.0 49 191.5 50 157.0 51 120.5 52 111.5 53 93.5 54 83.5 55 69.5 56 48.5 57 37.0 58 34.0 59 27.0 60 12.0 61 7.5 62 6.5 63 5.0 64 2.5 65 0.0 66 0.0 67 0.0 68 1.0 69 1.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.5 78 0.5 79 0.0 80 0.5 81 0.5 82 0.5 83 0.5 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27499999999999997 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.034999999999999996 25-29 0.075 30-34 0.05 35-39 0.034999999999999996 40-44 0.055 45-49 0.045 50-54 0.034999999999999996 55-59 0.075 60-64 0.034999999999999996 65-69 0.02 70-74 0.075 75-79 0.055 80-84 0.025 85-89 0.065 90-94 0.034999999999999996 95-99 0.025 100-104 0.075 105-109 0.06999999999999999 110-114 0.034999999999999996 115-119 0.065 120-124 0.075 125-129 0.065 130-134 0.075 135-139 0.075 140-144 0.034999999999999996 145-149 0.075 150-151 0.075 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.65 #Duplication Level Percentage of deduplicated Percentage of total 1 70.87952697708795 47.949999999999996 2 18.181818181818183 24.6 3 6.504065040650407 13.200000000000001 4 2.328159645232816 6.3 5 1.3303769401330376 4.5 6 0.5543237250554324 2.25 7 0.11086474501108648 0.525 8 0.07390983000739099 0.4 9 0.0 0.0 >10 0.03695491500369549 0.27499999999999997 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CAAAACACCTATCCATCTGTTTCTCTCTATTTCATCTCCATAGAAAAGAA 11 0.27499999999999997 No Hit GGCTCTGCCGGGTTATCTTGTGCTTATGAGCTTAGCAAGAACCCTTCTGT 8 0.2 No Hit ACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAAT 8 0.2 No Hit TGGCAACATACGATGAGGGCAGCAATATGAAGGGCTTTGGGCGAATAAAA 7 0.17500000000000002 No Hit AGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATC 7 0.17500000000000002 No Hit AACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAA 7 0.17500000000000002 No Hit CATGAGCCATCTGTCTTGATGACCTTTGATGGAGAATTAAAGCAACTCAT 6 0.15 No Hit CCGGCACTCCTCCCAGGAACATCCCAAAGTACTCCATTCCCACACAAACC 6 0.15 No Hit AGGCCTTGAAGGCGGGAGGATTACGACAGAGAGTTGAATTGGCCACCAAA 6 0.15 No Hit GTTATTTTTGGACCTGATGATACTCCTTGGGATGGAGGGACGTTTAAGTT 6 0.15 No Hit GGTGTTTAACGTAATTTATCTGGAAATTATAGTTATTGATTAGTACAAAA 6 0.15 No Hit ATATGGGAATCTCCTCCAAAGCCCTTCCAGATACTTCCTCACTATTATCA 6 0.15 No Hit AAGACCTAACCCCTTCTCTCCTTTCTTTCAAGCCCAAAACTCTCTCTCTC 6 0.15 No Hit GGGAAATGTTTGGCAACAAGTTTTGGAAATTCCCCTTTTCTGATTCTAGA 6 0.15 No Hit GATAGGTGCATTTTGTCGTGAAGATGATGAACTTTTTCTTAATTCTAACA 6 0.15 No Hit CTTTGTAATGAGCTAATCCAAGGATAACACTACAATGGCTACCTTTTCTC 6 0.15 No Hit GGGAAGCCTTACTCTGGACGTTATATAGGCTGCCTTGTTGGGGAAATACA 6 0.15 No Hit CTCTGACTGATCCAATATCCCGTGAGAATTATGAGAAATATGGTCATCCC 6 0.15 No Hit GCGGAGGGAAGAGGAACCAAGCCTTGCTGGGAATTGGAGCTAGTAGAACT 6 0.15 No Hit AGCTCTTCTCCAAGCACACTCGTTCACTGCTCTGTGATGCCTGTCACGCA 6 0.15 No Hit TGTACCGCTTAGCTTCACCGGAGGACTTGATGTTGCAATTATTTTCACTT 6 0.15 No Hit GTTGGATATGGTTAACCAGGATTCACGGAAGCCCGACAAGTCCTTGAAGA 5 0.125 No Hit GTTCTGCAACATCCATACACGTGTCTTTCATTCAAGGGGAACCCCAATGG 5 0.125 No Hit AGAGAACACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 5 0.125 No Hit CACATGGCAAGACTGGACAGTTGAGGTTTGTGGTCTGGTTAAAAGGCCAG 5 0.125 No Hit GTTCATCCAGAGGTCATTGATGGGAGACCTGGTACATTGGTGATCGAGTC 5 0.125 No Hit AGAAAAAGAATGCTGAGAACAGAAAGCTCATTTTCAACCGAGCTAAGCTA 5 0.125 No Hit GGATCTTTTGAAGAACCGATGGGAAGGGAAAGCCTTCAAAAACTGTGGCA 5 0.125 No Hit ATTTAGTGAGAAGCATGATATCAGACTTCTCCATGTGGGTTTAGTTTCAT 5 0.125 No Hit CTCGAATCGAATGATTTATCTACTGCTTCCAATATCAAGGCGAATGAACA 5 0.125 No Hit ATGAAATCTAAATCTTCTGTACCAGGATAGATCTACAATCAGTGGTCAGA 5 0.125 No Hit CACCAGACCAGCAGAGGCTGATTTTCGCCGGCAAGCAGCTTGAGGATGGC 5 0.125 No Hit CCTTCATGGCAATGGCTACTCAAGCCTCTCTCTTCACCCCCACGACCCTC 5 0.125 No Hit CTCGTCTCCACTCCTCTCCTTGCATGCACTACGACTCTAGTGCACACTCC 5 0.125 No Hit CCTATCTACAAAAAACCAAACCAAGCTCATCAAGACTGGCAGCCTATCTA 5 0.125 No Hit CTCAGATTCCTGCTGGTCAAGTTTTTCGAAGCTCAATTTCTGGTCATGTT 5 0.125 No Hit GGGAAGACAACAATTATTCTACAGTGGAAATTTCTGGGCTTGACATTGGA 5 0.125 No Hit GTGGCCAACAGGTTCAGGCCTGTCGTGCTGCTTATGTGAAAGCTATTGAG 5 0.125 No Hit GTAGTGTCTGAGGGTGCCATTAGTGCACCGATGTATTCGCTGAAAGGTCC 5 0.125 No Hit CCTGGGGCTAGAGGTGGTGAAACCTTTGATTCTAGCAATCGTGTTTATGT 5 0.125 No Hit GGATCCTAAGCCATTAGTGAGTACTAATACAGCAGATGCAAGTATTGACA 5 0.125 No Hit CCTTGCCGATCCAGTGGCCAACAATGCTTGGGCATACGCCACTAACTTCG 5 0.125 No Hit TGTTTATCCATGGATTGAAGCTTTAGCAAGGAATAGTGTGGGGTTAGAGG 5 0.125 No Hit CTTGATGTAACCAAGACTTGGCCCGAAGACATCTTGCCTTTGATGCCTGT 5 0.125 No Hit GGCCAGAAAGAGTTTGTGCAGATTGATCAGAATGATCTGTTCCCTTATCT 5 0.125 No Hit CAGAGATGGCGTTAGCGTTTGATGAATTCGGGAGACCCTTCGTAATCATA 5 0.125 No Hit ATGGATTTAATTTTGCATTTCTTTACATAGAAATTTCCTGAATATAAACC 5 0.125 No Hit ACCAGCTTGCCTCACTGTCTCTGAATGAAAATCTTGTTTCTTAGGTGTGT 5 0.125 No Hit GGAAGGGGTTGTTCAGAGGCAATTTGGTTAATGTGATTCGTGTTGCGCCT 5 0.125 No Hit CGAGGTGGGAGCAGATCTGGACCACCTTGGCTCTTAGGGGGACTAGAGAT 5 0.125 No Hit CGAATAAAAGCTTGGACTACTTCATTTTCGATAAAAAAACTAGAAATTCA 5 0.125 No Hit TGAGCTTGAAGCTGGCCTGTGCCATGCTTGTAGCGATGGTTGTTAGTGCA 5 0.125 No Hit AAAAGAGATGCACTGCATTTGCTTTCGTTAACCAGCAAATTGGTTTTCAA 5 0.125 No Hit GCTTGGACATGGATCAAGAACTGATGAGAAGTACCCACGATTGATAGAAC 5 0.125 No Hit GAAAACAAGCCGTTGGCAAAAGAGCTTGGCATAAAGGTAGTACCGACCTT 5 0.125 No Hit TAAAAAATCAAGCTGCCCTTATTCAGGACTATGATTGCATGTCAAATGCA 5 0.125 No Hit GGATGGTAGAGTAGCATCCTTAGCGTTGCTGGTTTACTTTCCTAACAATC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.1375 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.325 0.0 0.0 0.0 0.0 84-85 0.3375 0.0 0.0 0.0 0.0 86-87 0.375 0.0 0.0 0.0 0.0 88-89 0.4125 0.0 0.0 0.0 0.0 90-91 0.475 0.0 0.0 0.0 0.0 92-93 0.625 0.0 0.0 0.0 0.0 94-95 0.75 0.0 0.0 0.0 0.0 96-97 0.85 0.0 0.0 0.0 0.0 98-99 1.075 0.0 0.0 0.0 0.0 100-101 1.2125 0.0 0.0 0.0 0.0 102-103 1.375 0.0 0.0 0.0 0.0 104-105 1.5499999999999998 0.0 0.0 0.0 0.0 106-107 1.75 0.0 0.0 0.0 0.0 108-109 2.1375 0.0 0.0 0.0 0.0 110-111 2.4749999999999996 0.0 0.0 0.0 0.0 112-113 2.825 0.0 0.0 0.0 0.0 114-115 3.15 0.0 0.0 0.0 0.0 116-117 3.45 0.0 0.0 0.0 0.0 118-119 3.875 0.0 0.0 0.0 0.0 120-121 4.5125 0.0 0.0 0.0 0.0 122-123 5.025 0.0 0.0 0.0 0.0 124-125 5.5875 0.0 0.0 0.0 0.0 126-127 6.175 0.0 0.0 0.0 0.0 128-129 6.8375 0.0 0.0 0.0 0.0 130-131 7.375 0.0 0.0 0.0 0.0 132-133 7.7625 0.0 0.0 0.0 0.0 134-135 8.3875 0.0 0.0 0.0 0.0 136-137 9.287500000000001 0.0 0.0 0.0 0.0 138-139 10.037500000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTGAAG 10 0.006830828 145.0 9 AAAAAAA 75 3.4954483E-6 17.400002 30-34 >>END_MODULE Read 910928 spots for SRR13695455.sra Written 910928 spots for SRR13695455.sra Read 910928 spots for SRR13695455.sra Written 910928 spots for SRR13695455.sra Read 910928 spots for SRR13695455.sra Written 910928 spots for SRR13695455.sra Read 910928 spots for SRR13695455.sra Written 910928 spots for SRR13695455.sra Read 910928 spots for SRR13695455.sra Written 910928 spots for SRR13695455.sra Read 910928 spots for SRR13695455.sra Written 910928 spots for SRR13695455.sra Read 910928 spots for SRR13695455.sra Written 910928 spots for SRR13695455.sra Read 910928 spots for SRR13695455.sra Written 910928 spots for SRR13695455.sra Read 910928 spots for SRR13695455.sra Written 910928 spots for SRR13695455.sra Read 910938 spots for SRR13695455.sra Written 910938 spots for SRR13695455.sra Read 910928 spots for SRR13695455.sra Written 910928 spots for SRR13695455.sra Read 910928 spots for SRR13695455.sra Written 910928 spots for SRR13695455.sra Read 910928 spots for SRR13695455.sra Written 910928 spots for SRR13695455.sra Read 910928 spots for SRR13695455.sra Written 910928 spots for SRR13695455.sra Read 910928 spots for SRR13695455.sra Written 910928 spots for SRR13695455.sra Read 910928 spots for SRR13695455.sra Written 910928 spots for SRR13695455.sra Read 910928 spots for SRR13695455.sra Written 910928 spots for SRR13695455.sra Read 910928 spots for SRR13695455.sra Written 910928 spots for SRR13695455.sra Read 910928 spots for SRR13695455.sra Written 910928 spots for SRR13695455.sra Read 910928 spots for SRR13695455.sra Written 910928 spots for SRR13695455.sra SRR ids: ['SRR13695455.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_vza1j7e5 SRR13695455.sra spots: 18218570 blocks: [[1, 910928], [910929, 1821856], [1821857, 2732784], [2732785, 3643712], [3643713, 4554640], [4554641, 5465568], [5465569, 6376496], [6376497, 7287424], [7287425, 8198352], [8198353, 9109280], [9109281, 10020208], [10020209, 10931136], [10931137, 11842064], [11842065, 12752992], [12752993, 13663920], [13663921, 14574848], [14574849, 15485776], [15485777, 16396704], [16396705, 17307632], [17307633, 18218570]] SRR13695455 file size 6169766 SRR13695455 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695455 SRR13695455_1.fastq SRR13695455_2.fastq Input file: SRR13695455_1.fastq Paired file: SRR13695455_2.fastq trimmed: SRR13695455-trimmed-pair1.fastq, SRR13695455-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 04:31:59 2025 >> started Wed Feb 12 04:32:21 2025 >> done (22.055s) 18218570 read pairs processed; of these: 126 ( 0.00%) short read pairs filtered out after trimming by size control 1135 ( 0.01%) empty read pairs filtered out after trimming by size control 18217309 (99.99%) read pairs available; of these: 2327056 (12.77%) trimmed read pairs available after processing 15890253 (87.23%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 5 0.00% 19 4 0.00% 20 2 0.00% 21 1 0.00% 22 3 0.00% 23 2 0.00% 24 2 0.00% 25 1 0.00% 26 5 0.00% 27 2 0.00% 28 3 0.00% 29 16 0.00% 30 5 0.00% 31 4 0.00% 32 10 0.00% 33 4 0.00% 34 3 0.00% 35 9 0.00% 36 4 0.00% 37 6 0.00% 38 11 0.00% 39 5 0.00% 40 11 0.00% 41 19 0.00% 42 14 0.00% 43 22 0.00% 44 25 0.00% 45 19 0.00% 46 16 0.00% 47 34 0.00% 48 41 0.00% 49 57 0.00% 50 57 0.00% 51 72 0.00% 52 86 0.00% 53 81 0.00% 54 75 0.00% 55 100 0.00% 56 98 0.00% 57 130 0.00% 58 162 0.00% 59 218 0.00% 60 310 0.00% 61 311 0.00% 62 348 0.00% 63 396 0.00% 64 414 0.00% 65 488 0.00% 66 526 0.00% 67 624 0.00% 68 674 0.00% 69 891 0.00% 70 1028 0.01% 71 1257 0.01% 72 1317 0.01% 73 1544 0.01% 74 1802 0.01% 75 1971 0.01% 76 2284 0.01% 77 2455 0.01% 78 2702 0.01% 79 3238 0.02% 80 3631 0.02% 81 4045 0.02% 82 4590 0.03% 83 4874 0.03% 84 5762 0.03% 85 6296 0.03% 86 6875 0.04% 87 7011 0.04% 88 7941 0.04% 89 8460 0.05% 90 9162 0.05% 91 9820 0.05% 92 10818 0.06% 93 11714 0.06% 94 12488 0.07% 95 13254 0.07% 96 14572 0.08% 97 15092 0.08% 98 15750 0.09% 99 16098 0.09% 100 17448 0.10% 101 17911 0.10% 102 19169 0.11% 103 20215 0.11% 104 20898 0.11% 105 22211 0.12% 106 23430 0.13% 107 24357 0.13% 108 25220 0.14% 109 25820 0.14% 110 26430 0.15% 111 27597 0.15% 112 28700 0.16% 113 29818 0.16% 114 30558 0.17% 115 32369 0.18% 116 33276 0.18% 117 34413 0.19% 118 35729 0.20% 119 36671 0.20% 120 37341 0.20% 121 38089 0.21% 122 38674 0.21% 123 39984 0.22% 124 41165 0.23% 125 41855 0.23% 126 43459 0.24% 127 44444 0.24% 128 45181 0.25% 129 45893 0.25% 130 46735 0.26% 131 47522 0.26% 132 48429 0.27% 133 49115 0.27% 134 50221 0.28% 135 50990 0.28% 136 50870 0.28% 137 53021 0.29% 138 53340 0.29% 139 55674 0.31% 140 55947 0.31% 141 56640 0.31% 142 57587 0.32% 143 57341 0.31% 144 58941 0.32% 145 59822 0.33% 146 60072 0.33% 147 60833 0.33% 148 63266 0.35% 149 63985 0.35% 150 64103 0.35% 151 15890253 87.23% 18217309 reads passed initial QC criterion=sequence-density sequence-density=0.41 sequence-density-rank=1 fanout-score=2.00 fanout-score-rank=28 prefix-density=0.41 prefix-fanout=2.0 sequence=TGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGG criterion=fanout-score sequence-density=0.02 sequence-density-rank=32 fanout-score=229.57 fanout-score-rank=1 prefix-density=0.27 prefix-fanout=14.9 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAG criterion=sequence-density sequence-density=1.20 sequence-density-rank=1 fanout-score=2.07 fanout-score-rank=29 prefix-density=1.21 prefix-fanout=2.1 sequence=CTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAGTGAACAAACAAAAGTGTCCGTGAGTGTCTTTATCTTTGTGTCTTCTTCA criterion=fanout-score sequence-density=0.17 sequence-density-rank=19 fanout-score=27.21 fanout-score-rank=1 prefix-density=0.43 prefix-fanout=10.6 sequence=AAGAAAAGAAAA SRR13695455 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 04:33:45 Started mapping on | Feb 12 04:33:45 Finished on | Feb 12 04:36:15 Mapping speed, Million of reads per hour | 437.22 Number of input reads | 18217309 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 16891056 Uniquely mapped reads % | 92.72% Average mapped length | 294.26 Number of splices: Total | 16657743 Number of splices: Annotated (sjdb) | 16281667 Number of splices: GT/AG | 16328172 Number of splices: GC/AG | 257078 Number of splices: AT/AC | 10168 Number of splices: Non-canonical | 62325 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 2.82 Insertion rate per base | 0.02% Insertion average length | 2.33 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 395702 % of reads mapped to multiple loci | 2.17% Number of reads mapped to too many loci | 93785 % of reads mapped to too many loci | 0.51% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.46% % of reads unmapped: other | 0.14% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 930751 930751 930751 N_multimapping 395702 395702 395702 N_noFeature 683990 16397536 979793 N_ambiguous 309904 1944 110837 UnstrandedReadsAssigned:15897162 PositiveStrandReadsAssigned:491576 NegativeStrandReadsAssigned:15800426 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695455 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695455-trimmed-pair1.fastq SRR13695455-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 18,217,309 reads, 15,825,484 reads pseudoaligned [quant] estimated average fragment length: 246.576 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,067 rounds 52401 SRR13695455.ke.tsv 34699 SRR13695455.se.tsv 87100 total ==> SRR13695455.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1772.42 495 14.4281 Potri.005G024800.1.v4.1 1035 789.424 316 20.6799 Potri.004G059700.1.v4.1 961 715.495 5 0.361023 Potri.007G009000.2.v4.1 1416 1170.42 0 0 Potri.003G141000.2.v4.1 2943 2697.42 716 13.7131 Potri.016G087400.1.v4.1 270 86.71 798 475.45 Potri.015G069301.1.v4.1 564 327.204 0 0 Potri.010G195200.1.v4.1 1773 1527.42 62 2.09702 Potri.012G127500.1.v4.1 977 731.475 74 5.22641 ==> SRR13695455.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 196 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 284 Potri.001G212900.v4.1 2 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1 SRR13695455 completed mapping pipeline successfully