Starting /dee2/code/volunteer_pipeline.sh SRR13695456 current disk space = 3049195089920 free memory = 1334260840 SRR13695456 SRAfilesize 1cdc560f0f5022c3d8f0eaeaccde7555 SRR13695456.sra SRR13695456.sra file validated SRR13695456 is paired end SRR13695456 is conventional basespace SRR13695456 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695456_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.522 37.0 37.0 37.0 37.0 37.0 2 36.25625 37.0 37.0 37.0 37.0 37.0 3 36.5265 37.0 37.0 37.0 37.0 37.0 4 36.57 37.0 37.0 37.0 37.0 37.0 5 36.6275 37.0 37.0 37.0 37.0 37.0 6 36.6125 37.0 37.0 37.0 37.0 37.0 7 36.523 37.0 37.0 37.0 37.0 37.0 8 36.55 37.0 37.0 37.0 37.0 37.0 9 36.5715 37.0 37.0 37.0 37.0 37.0 10-14 36.568599999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.5269 37.0 37.0 37.0 37.0 37.0 20-24 36.5523 37.0 37.0 37.0 37.0 37.0 25-29 36.4988 37.0 37.0 37.0 37.0 37.0 30-34 36.49739999999999 37.0 37.0 37.0 37.0 37.0 35-39 36.461200000000005 37.0 37.0 37.0 37.0 37.0 40-44 36.4373 37.0 37.0 37.0 37.0 37.0 45-49 36.4342 37.0 37.0 37.0 37.0 37.0 50-54 36.39640000000001 37.0 37.0 37.0 37.0 37.0 55-59 36.380399999999995 37.0 37.0 37.0 37.0 37.0 60-64 36.364599999999996 37.0 37.0 37.0 37.0 37.0 65-69 36.252599999999994 37.0 37.0 37.0 37.0 37.0 70-74 36.3395 37.0 37.0 37.0 37.0 37.0 75-79 36.2999 37.0 37.0 37.0 37.0 37.0 80-84 36.2642 37.0 37.0 37.0 37.0 37.0 85-89 36.225100000000005 37.0 37.0 37.0 37.0 37.0 90-94 36.163599999999995 37.0 37.0 37.0 37.0 37.0 95-99 36.1163 37.0 37.0 37.0 37.0 37.0 100-104 36.104400000000005 37.0 37.0 37.0 37.0 37.0 105-109 36.07690000000001 37.0 37.0 37.0 37.0 37.0 110-114 36.0429 37.0 37.0 37.0 37.0 37.0 115-119 36.0173 37.0 37.0 37.0 37.0 37.0 120-124 35.9767 37.0 37.0 37.0 37.0 37.0 125-129 35.9898 37.0 37.0 37.0 37.0 37.0 130-134 35.9147 37.0 37.0 37.0 37.0 37.0 135-139 35.8144 37.0 37.0 37.0 37.0 37.0 140-144 35.781400000000005 37.0 37.0 37.0 37.0 37.0 145-149 35.655199999999994 37.0 37.0 37.0 37.0 37.0 150-151 35.596999999999994 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 1.0 22 1.0 23 0.0 24 2.0 25 3.0 26 5.0 27 12.0 28 13.0 29 23.0 30 19.0 31 33.0 32 50.0 33 79.0 34 126.0 35 308.0 36 3006.0 37 319.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 33.925 12.85 8.799999999999999 44.425 2 19.749058971141782 15.131744040150567 37.66624843161857 27.452948557089087 3 16.45 17.375 28.1 38.074999999999996 4 22.900000000000002 24.875 23.65 28.575 5 23.575 32.275 24.075 20.075000000000003 6 19.725 35.825 23.875 20.575 7 15.45 26.950000000000003 41.125 16.475 8 17.1 25.874999999999996 33.6 23.425 9 16.7 24.5 35.35 23.45 10-14 19.75 30.104999999999997 27.560000000000002 22.585 15-19 19.650000000000002 29.020000000000003 27.83 23.5 20-24 19.675 28.92 27.58 23.825 25-29 19.689999999999998 28.439999999999998 28.105000000000004 23.765 30-34 19.825 29.349999999999998 27.224999999999998 23.599999999999998 35-39 20.22 29.09 27.529999999999998 23.16 40-44 19.71 28.59 29.049999999999997 22.650000000000002 45-49 20.5 28.865000000000002 28.37 22.264999999999997 50-54 19.555 28.67 27.875 23.9 55-59 19.81 29.29 27.67 23.23 60-64 20.630000000000003 29.01 27.63 22.73 65-69 19.275000000000002 28.37 29.28 23.075000000000003 70-74 19.905 29.37 27.584999999999997 23.14 75-79 19.7 27.884999999999998 28.804999999999996 23.61 80-84 20.669999999999998 28.12 28.155 23.055 85-89 20.69 28.68 27.915 22.715 90-94 20.775 28.4 27.47 23.355 95-99 20.21 28.87 27.779999999999998 23.14 100-104 20.669999999999998 29.255 26.935 23.14 105-109 19.885 28.88 27.634999999999998 23.599999999999998 110-114 20.415 28.939999999999998 27.42 23.225 115-119 20.16 28.405 27.725 23.71 120-124 20.419999999999998 28.015 27.884999999999998 23.68 125-129 20.544999999999998 28.29 27.150000000000002 24.015 130-134 21.105 28.849999999999998 27.18 22.865 135-139 20.75 27.98 27.67 23.599999999999998 140-144 21.17 27.61 27.91 23.31 145-149 20.635 28.93 27.075 23.36 150-151 21.762500000000003 29.049999999999997 26.650000000000002 22.537499999999998 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 0.5 19 2.0 20 2.0 21 0.5 22 0.5 23 0.0 24 1.5 25 4.5 26 6.5 27 5.0 28 8.0 29 23.0 30 25.0 31 23.0 32 31.0 33 49.0 34 77.0 35 91.5 36 107.0 37 134.5 38 156.5 39 166.5 40 179.0 41 219.5 42 249.0 43 252.0 44 256.5 45 281.0 46 259.5 47 224.0 48 230.5 49 193.0 50 161.5 51 128.5 52 86.5 53 82.0 54 70.5 55 50.5 56 47.0 57 40.0 58 22.5 59 14.0 60 12.0 61 10.5 62 7.5 63 4.5 64 2.0 65 0.0 66 0.0 67 0.5 68 0.5 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.375 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.60000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 68.16816816816817 45.4 2 20.12012012012012 26.8 3 7.657657657657657 15.299999999999999 4 2.59009009009009 6.9 5 0.9009009009009009 3.0 6 0.2627627627627628 1.05 7 0.22522522522522523 1.05 8 0.0 0.0 9 0.03753753753753754 0.22499999999999998 >10 0.03753753753753754 0.27499999999999997 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCTATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGT 11 0.27499999999999997 No Hit ATCTACGTCATTACCAAAGACAGAGACCAATCCCATGCCCGTTATGACAA 9 0.22499999999999998 No Hit CTTAAGTTATGGCAAGAGGAGTGTCATTCTAGTCTAATTTAAATGATTTT 7 0.17500000000000002 No Hit CTCTACTAGGCAAGTTATATCATCTCGAAAATGAGTCTCAAGAACTGAAA 7 0.17500000000000002 No Hit CAGAGGTTGTTCAGGCTCCCCAAGAAAATGCAGTATATGCCATCCAATCC 7 0.17500000000000002 No Hit CCTGTCACGAGACACCTCCCTCTCCCGTTCAGTGTGCTGATCTTCTCGTG 7 0.17500000000000002 No Hit ACCAAGTTTCACTTCAGTGCCTCCGTATTCAGGCAAAAGGACAGTTTCTC 7 0.17500000000000002 No Hit CACCAATCTATGTACATACTGTTGCAGACGGCATGGACAATTTAGAAGTT 7 0.17500000000000002 No Hit CCTGCAGCTTGTGCACAGAGTTTGGCCATTCCATCCAGGGTCACAGCACG 6 0.15 No Hit CCCATAGGCAAACCAGGGTGACCTGAATTAGCCTTTTCGACAGCATCAAT 6 0.15 No Hit GCTGGTTTGAAAAGAAAAAAAATCTCTGCTGAAGTTATTTTTCTTTAAAA 6 0.15 No Hit AGCCAAAGCAACCGGAGGGCCTTCATTTTCTGGACTCCCCTCGTCCTCAT 6 0.15 No Hit GTTGATTCTTCTCTTCCAAATTCATACTAATATCTTGTACATCTGAACTC 6 0.15 No Hit GGAGCAAGAAGATGAAGAATTGAAGGATTTCTTGCATAAATAGTGAGAGT 6 0.15 No Hit GCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGC 6 0.15 No Hit GTGGTAAAAGCATTCTTCGCCGCAGAGAAAACTGTTTGACATTGAAATGA 5 0.125 No Hit GTGTAGTCGAGCAAAAATGTGCTCCTCAAAAGTCTGTCTATCCTGCTTAA 5 0.125 No Hit CCAACTCAATAAAGCTCATATTTTTGCCTAAAAAACTGGACTTGAGAGAT 5 0.125 No Hit CGTTCCTGCCTCATTTTCACCATCATCTTCCGAATCATCTTTAGCATCTA 5 0.125 No Hit ACAAAAACTAGTGCTTGAAAATTGACTTTACAACGTAGACGCTGGATGTC 5 0.125 No Hit GGATCAGAAGACATCGTGTCCCAGTGATCAAAAACACATTGTGGGAAAGC 5 0.125 No Hit TGGGAGACGGAGGGGCTTGTCTGAGGGCCTCTTGGGCTCCTGGATCTGGT 5 0.125 No Hit CCCATATTCACATCGACCCAGCATCATACATCAGAAACTAACTACTGCCA 5 0.125 No Hit ATCCATAGGTAATTGTGTAGAATTGGTAGGAAAAGGATCCATAGGTGATT 5 0.125 No Hit CTTGGTAGTCAATGCACAAAGTTTCCCAGGAATCTCTCTGTACCTAACCT 5 0.125 No Hit ATCCGATCTACTCTCCTTGATAGAAGAAACCTTGAGATGAATTTCCCCAA 5 0.125 No Hit TGGTAAACTAGATTCATAGTACAACCACAAATGGACATACTCTAGAAAGA 5 0.125 No Hit TGCAATTCTGTGAGTTGGTTCCCTGTCTCACTGACAGAACCTTCATGTAA 5 0.125 No Hit GTCCATTTCTTTGAGCCTCACTCGAGTTAGCTCACTCCCTTGTTGCAATT 5 0.125 No Hit CACCAGTCTAAACCATTACCCTCCCCATGAACTTCTGTTTGACTCTGATC 5 0.125 No Hit CAGGGATGAGCTTGATCCTCATGCTAACACGGCCGAACAAGTACTTGGCC 5 0.125 No Hit CTCTGTGGTCAGCTCACTTACAATTTGTTCCAGATCACCTTCTGGCAGAA 5 0.125 No Hit TATGGATTCTCAGGCTCCCTTGCTACAAAGAAAGGCTCGCCACCGTAGCA 5 0.125 No Hit GGGTAGATTGGGTCAGTTACCTCACCGAGTGGCCCGCCAGCAATTCTGTA 5 0.125 No Hit CGCTTCATAACAAATACTCAAGACTGATTGTCATCACTTTCTTCTTTTTT 5 0.125 No Hit TCCTGGTTTTGTACACGGTGCAAGCCAACAGCAGTGGAATTGCAAGGCTA 5 0.125 No Hit GTCAAACGCACAGCATAAGGTAGATCCGACTCGCCAAGGGCGTAGAGTCG 5 0.125 No Hit CTCCATATCAGTTGCGGGCACCTTGTGGACCTTTCCCTTGTTGAAGACGT 5 0.125 No Hit CTTGTACATTTGTGAAGGTCTTGCAATTTTTGTTGAAAGATCCTTCTTGT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0125 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.175 0.0 0.0 0.0 0.0 78-79 0.225 0.0 0.0 0.0 0.0 80-81 0.25 0.0 0.0 0.0 0.0 82-83 0.3125 0.0 0.0 0.0 0.0 84-85 0.4125 0.0 0.0 0.0 0.0 86-87 0.44999999999999996 0.0 0.0 0.0 0.0 88-89 0.5125 0.0 0.0 0.0 0.0 90-91 0.625 0.0 0.0 0.0 0.0 92-93 0.875 0.0 0.0 0.0 0.0 94-95 1.025 0.0 0.0 0.0 0.0 96-97 1.225 0.0 0.0 0.0 0.0 98-99 1.35 0.0 0.0 0.0 0.0 100-101 1.5499999999999998 0.0 0.0 0.0 0.0 102-103 1.7375 0.0 0.0 0.0 0.0 104-105 1.9 0.0 0.0 0.0 0.0 106-107 2.0999999999999996 0.0 0.0 0.0 0.0 108-109 2.325 0.0 0.0 0.0 0.0 110-111 2.4749999999999996 0.0 0.0 0.0 0.0 112-113 2.6625 0.0 0.0 0.0 0.0 114-115 2.7750000000000004 0.0 0.0 0.0 0.0 116-117 2.9749999999999996 0.0 0.0 0.0 0.0 118-119 3.2375 0.0 0.0 0.0 0.0 120-121 3.6875 0.0 0.0 0.0 0.0 122-123 4.324999999999999 0.0 0.0 0.0 0.0 124-125 4.862500000000001 0.0 0.0 0.0 0.0 126-127 5.25 0.0 0.0 0.0 0.0 128-129 5.6875 0.0 0.0 0.0 0.0 130-131 6.2125 0.0 0.0 0.0 0.0 132-133 6.525 0.0 0.0 0.0 0.0 134-135 6.862500000000001 0.0 0.0 0.0 0.0 136-137 7.375 0.0 0.0 0.0 0.0 138-139 8.1625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCTGGT 10 0.006830828 145.0 1 AAACCGA 10 0.006830828 145.0 8 AACCGAT 10 0.006830828 145.0 9 TAAACCG 10 0.006830828 145.0 7 TGGTAAA 10 0.006830828 145.0 4 CTGGTAA 10 0.006830828 145.0 3 GGTAAAC 10 0.006830828 145.0 5 >>END_MODULE SRR13695456 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695456_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.25325 37.0 37.0 37.0 37.0 37.0 2 36.332 37.0 37.0 37.0 37.0 37.0 3 36.0935 37.0 37.0 37.0 37.0 37.0 4 36.2085 37.0 37.0 37.0 37.0 37.0 5 36.3135 37.0 37.0 37.0 37.0 37.0 6 36.2225 37.0 37.0 37.0 37.0 37.0 7 36.419 37.0 37.0 37.0 37.0 37.0 8 36.384 37.0 37.0 37.0 37.0 37.0 9 36.3315 37.0 37.0 37.0 37.0 37.0 10-14 36.3231 37.0 37.0 37.0 37.0 37.0 15-19 36.32289999999999 37.0 37.0 37.0 37.0 37.0 20-24 36.288250000000005 37.0 37.0 37.0 37.0 37.0 25-29 36.2383 37.0 37.0 37.0 37.0 37.0 30-34 36.203149999999994 37.0 37.0 37.0 37.0 37.0 35-39 36.209799999999994 37.0 37.0 37.0 37.0 37.0 40-44 36.2594 37.0 37.0 37.0 37.0 37.0 45-49 36.20245 37.0 37.0 37.0 37.0 37.0 50-54 36.1763 37.0 37.0 37.0 37.0 37.0 55-59 36.1503 37.0 37.0 37.0 37.0 37.0 60-64 36.076100000000004 37.0 37.0 37.0 37.0 37.0 65-69 36.06555 37.0 37.0 37.0 37.0 37.0 70-74 36.065999999999995 37.0 37.0 37.0 37.0 37.0 75-79 36.0613 37.0 37.0 37.0 37.0 37.0 80-84 35.980149999999995 37.0 37.0 37.0 37.0 37.0 85-89 35.85885 37.0 37.0 37.0 37.0 37.0 90-94 35.9131 37.0 37.0 37.0 37.0 37.0 95-99 35.857850000000006 37.0 37.0 37.0 37.0 37.0 100-104 35.918400000000005 37.0 37.0 37.0 37.0 37.0 105-109 35.9187 37.0 37.0 37.0 37.0 37.0 110-114 35.824400000000004 37.0 37.0 37.0 37.0 37.0 115-119 35.78195 37.0 37.0 37.0 37.0 37.0 120-124 35.6767 37.0 37.0 37.0 37.0 37.0 125-129 35.76805 37.0 37.0 37.0 37.0 37.0 130-134 35.5292 37.0 37.0 37.0 34.6 37.0 135-139 35.574400000000004 37.0 37.0 37.0 37.0 37.0 140-144 35.439499999999995 37.0 37.0 37.0 37.0 37.0 145-149 35.3038 37.0 37.0 37.0 32.2 37.0 150-151 35.23525 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 1.0 16 1.0 17 0.0 18 0.0 19 0.0 20 3.0 21 0.0 22 0.0 23 1.0 24 5.0 25 8.0 26 7.0 27 10.0 28 9.0 29 18.0 30 29.0 31 31.0 32 63.0 33 97.0 34 200.0 35 573.0 36 2713.0 37 230.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 33.675770483588074 22.650964670508642 11.550989726885492 32.12227511901779 2 27.650000000000002 26.025 32.675 13.65 3 19.125 27.750000000000004 31.674999999999997 21.45 4 23.549999999999997 33.225 25.275 17.95 5 25.1 36.325 21.349999999999998 17.224999999999998 6 20.225 40.0 22.975 16.8 7 19.1 23.875 38.824999999999996 18.2 8 21.575 25.825 28.050000000000004 24.55 9 21.8 25.025 30.7 22.475 10-14 23.005 29.925 26.55 20.52 15-19 21.34 29.575000000000003 27.605 21.48 20-24 23.28582145536384 28.657164291072768 27.47686921730433 20.580145036259065 25-29 22.471235617808905 27.99899949974988 28.53926963481741 20.990495247623812 30-34 21.867653678787576 27.89476316710849 29.105186815385387 21.13239633871855 35-39 23.39701910573172 28.09842952885866 28.218465539661896 20.286085825747723 40-44 22.554021608643456 28.59643857543017 28.331332533013203 20.518207282913163 45-49 22.39283749312259 28.13484719651878 28.449957485119793 21.02235782523883 50-54 22.88186455936781 28.423527058117436 28.3184955486646 20.376112833850154 55-59 22.99649824912456 27.39869934967484 28.424212106053027 21.180590295147574 60-64 22.656797039111733 27.9333800140042 28.808642592777833 20.601180354106233 65-69 22.718407761164176 27.279091863779563 29.11936790518578 20.883132469870482 70-74 22.411205602801402 27.518759379689843 29.099549774887446 20.97048524262131 75-79 22.233893557422967 27.62605042016807 28.55642256902761 21.583633453381353 80-84 23.315828957239308 28.912228057014254 27.246811702925733 20.525131282820706 85-89 22.655194837676955 28.332749737381825 28.1426641988895 20.86939122605172 90-94 23.772131639491846 29.278783635090527 27.173151945583673 19.775932779833948 95-99 23.495873968492123 28.30207551887972 27.776944236059016 20.425106276569142 100-104 22.501250625312657 28.489244622311155 28.394197098549274 20.615307653826914 105-109 23.336668334167083 28.394197098549274 27.533766883441718 20.735367683841922 110-114 23.181954586375912 29.143743122936883 27.908372511753527 19.765929778933682 115-119 23.710669801410635 28.70791856335351 27.662448101645744 19.918963533590116 120-124 23.76688344172086 27.953976988494244 27.818909454727365 20.460230115057527 125-129 23.575609024060828 28.22770246610975 27.56240308138662 20.6342854284428 130-134 23.84192096048024 28.53926963481741 27.658829414707352 19.959979989995 135-139 23.391695847923963 28.254127063531765 27.358679339669834 20.995497748874435 140-144 24.07222166649995 28.37851355406622 27.31819545863759 20.231069320796237 145-149 25.097548774387196 28.269134567283643 26.973486743371687 19.65982991495748 150-151 27.101050525262632 28.039019509754876 26.450725362681343 18.409204602301152 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.0 9 0.5 10 0.0 11 0.0 12 0.5 13 0.5 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.5 21 0.5 22 0.5 23 1.0 24 1.5 25 2.5 26 5.5 27 7.5 28 9.5 29 14.0 30 19.0 31 27.0 32 42.0 33 51.0 34 60.5 35 85.5 36 100.0 37 108.0 38 141.0 39 189.0 40 207.0 41 227.5 42 265.5 43 280.0 44 277.0 45 268.0 46 256.5 47 250.5 48 231.0 49 189.0 50 155.5 51 116.5 52 83.0 53 71.0 54 63.0 55 51.0 56 40.5 57 32.5 58 16.0 59 12.0 60 18.0 61 10.5 62 2.0 63 2.0 64 2.0 65 0.5 66 0.5 67 0.5 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 1.0 86 1.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22499999999999998 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.025 25-29 0.05 30-34 0.034999999999999996 35-39 0.03 40-44 0.04 45-49 0.034999999999999996 50-54 0.03 55-59 0.05 60-64 0.03 65-69 0.015 70-74 0.05 75-79 0.04 80-84 0.025 85-89 0.045 90-94 0.03 95-99 0.025 100-104 0.05 105-109 0.05 110-114 0.03 115-119 0.045 120-124 0.05 125-129 0.045 130-134 0.05 135-139 0.05 140-144 0.03 145-149 0.05 150-151 0.05 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.0 #Duplication Level Percentage of deduplicated Percentage of total 1 68.84328358208955 46.125 2 19.55223880597015 26.200000000000003 3 7.686567164179105 15.45 4 2.5 6.7 5 0.8208955223880596 2.75 6 0.2611940298507463 1.05 7 0.22388059701492538 1.05 8 0.0746268656716418 0.4 9 0.0 0.0 >10 0.0373134328358209 0.27499999999999997 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCC 11 0.27499999999999997 No Hit CAGAACACAAACACACAACATCGTTACTATCGCCCTCTCAAATTCTCCCT 8 0.2 No Hit CAGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 8 0.2 No Hit CTCTTGAGATTCCCATCATTGTTATTTTTCTGCTTTCAATCTCATTGATT 7 0.17500000000000002 No Hit GCTGAGATGGCAAGGCTGAGGGAGCTAAACACCCTCAAGGGGCATGTTGA 7 0.17500000000000002 No Hit GAAGAAAGAGCTGGCAGCATGGACAATAATGAATGTTCAGGTGGTCTATT 7 0.17500000000000002 No Hit CCGGTATTCTTCTTCCTGAGAAAACCTCCAAGCTGAACTCTGGAAAAGTT 7 0.17500000000000002 No Hit GAGACAGACTGAGAGTGTTTCAGAATTCAGGAATGGCTTCCACTTCTTCT 7 0.17500000000000002 No Hit CACCGAGAGTAAAATTAAAAGGGAGCTTGAGTCTTATGGACCAATAAAAC 7 0.17500000000000002 No Hit ATGCAATCCTTTCCAGTATATTCTCCTTGATAACAGATACACGTAGCCGC 6 0.15 No Hit TGGATGTCAGATTATTCAGTAGTTGGAGTATCTTTGTTATGTCGTTTACG 6 0.15 No Hit TTTTAAAATAAAAAACATACCGCTCTGACGCTGGAATGGAAAAGGTTGAA 6 0.15 No Hit CTATGCCAAAGATATCAGGGGTGGTGAAGCTGGATGTGTCCAAAGGAGAG 6 0.15 No Hit AGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATC 6 0.15 No Hit CAAAAGCTTTTGAAGTTCGTAGACATATCACATTTGTGTAGAGGGCCACA 6 0.15 No Hit CATCTCTCAATTAAATCAATTTCCATGCAAGACTCTCTCTTCAAAAACAC 6 0.15 No Hit AACAGACCTCAAAGAAAAATTGTCAATGGCTTCTCTTGTAGAAGGTACTT 5 0.125 No Hit CAACTTTGGCTGATATGAAAAGAGTATGGCTTTCCAGGAAGTTTACCTAC 5 0.125 No Hit AAAACAATGATTTACAAATCTCTTGAAAGTTGTACATGTTTGACACTTAA 5 0.125 No Hit AGCAGTCAGATAGAGAAAGCCCTCCATGTTCTTGGCAAGGAAGAAATTCA 5 0.125 No Hit CGAGGGAGTATTATGCCATAGCTATTCGAGCTACCCGAATTAAGATGGCA 5 0.125 No Hit CTGAGAAAGGCTAGTGTCTTTCTTTTCTTTTCCTTCCTTGCATTTCCTCT 5 0.125 No Hit GTTCAGGGGAAACGATAAGCCTCCAGCACATCTGGGCTCAAGCAGGGATT 5 0.125 No Hit GGATAAAGGAGAGTACGAGGAGTTTTGTAACAGGGAATTGAAACCTGGAT 5 0.125 No Hit GGAATGAGGAAAAACAGAAAGCAAAAGTAAGGGAAAAGCTTGACAAGTGC 5 0.125 No Hit ATCAAGTCTCCTGCTGCCAAAAGGGCCAAGAAATAGAGACTTGGTCCTGG 5 0.125 No Hit CCTTAGACCCTCAACCAAACCCAGCTCCCTCCATGGTCTCCTCAGCTTCT 5 0.125 No Hit GACTACAGTTCTGCAGAAAGTGTCTACTCTTCACGGAAAAGGATAGATGG 5 0.125 No Hit CTTACAAATTTTAGTGGACCTGGTGTTGGATTCGGTTTCGGTGTTGGTTG 5 0.125 No Hit CTCTTGGATGCGTCTTCCCCGAGCTCTTGTCCCGCAACGGTGTCAAGTTC 5 0.125 No Hit CTACCATGCCGCCACCACCATCGTCGTCGTCGTCATCATCATCAACGGCG 5 0.125 No Hit CTCCAAGGCAAGGTATGATGAAATTGTCAAGGAAGTGTCCTCCTACTTGA 5 0.125 No Hit GGATGATAATGGCAGTTACCACAGAGCAGAGGAAGGCAGCACTAGACCTA 5 0.125 No Hit CACTCACCTGAAACCACATTGCAGAGTCCCCATAATACTGTTATGGCTGG 5 0.125 No Hit CTCTGCAGTCGCTACGTCAAGACTTACAAATATACCATGGAGCGTGATGC 5 0.125 No Hit GACTGCCAAGCCCAGGCTCCTTGAGCCCGTGTACATGGTGGAGATCCAAG 5 0.125 No Hit GTTGCAGAATGAGGTGGCTGAACTACCTTCAACCCAATCTTAAGCGCGGT 5 0.125 No Hit AGGGGCCCCACAGGGATTCCTTGCTGAATGCTATATGCATAGAGCTTATG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0125 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.175 0.0 0.0 0.0 0.0 78-79 0.225 0.0 0.0 0.0 0.0 80-81 0.25 0.0 0.0 0.0 0.0 82-83 0.3125 0.0 0.0 0.0 0.0 84-85 0.4125 0.0 0.0 0.0 0.0 86-87 0.44999999999999996 0.0 0.0 0.0 0.0 88-89 0.5125 0.0 0.0 0.0 0.0 90-91 0.625 0.0 0.0 0.0 0.0 92-93 0.875 0.0 0.0 0.0 0.0 94-95 1.0125 0.0 0.0 0.0 0.0 96-97 1.2000000000000002 0.0 0.0 0.0 0.0 98-99 1.325 0.0 0.0 0.0 0.0 100-101 1.525 0.0 0.0 0.0 0.0 102-103 1.7125 0.0 0.0 0.0 0.0 104-105 1.875 0.0 0.0 0.0 0.0 106-107 2.075 0.0 0.0 0.0 0.0 108-109 2.3 0.0 0.0 0.0 0.0 110-111 2.4749999999999996 0.0 0.0 0.0 0.0 112-113 2.6625 0.0 0.0 0.0 0.0 114-115 2.7750000000000004 0.0 0.0 0.0 0.0 116-117 2.9749999999999996 0.0 0.0 0.0 0.0 118-119 3.2375 0.0 0.0 0.0 0.0 120-121 3.6875 0.0 0.0 0.0 0.0 122-123 4.324999999999999 0.0 0.0 0.0 0.0 124-125 4.862500000000001 0.0 0.0 0.0 0.0 126-127 5.225 0.0 0.0 0.0 0.0 128-129 5.6625 0.0 0.0 0.0 0.0 130-131 6.2 0.0 0.0 0.0 0.0 132-133 6.55 0.0 0.0 0.0 0.0 134-135 6.887499999999999 0.0 0.0 0.0 0.0 136-137 7.425 0.0 0.0 0.0 0.0 138-139 8.2125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTTAAA 10 0.006830828 145.0 3 CGCTTTA 10 0.006830828 145.0 1 AGAGCCG 10 0.006830828 145.0 9 AAAGAGC 10 0.006830828 145.0 7 CCCTAAT 10 0.006830828 145.0 1 >>END_MODULE Read 978638 spots for SRR13695456.sra Written 978638 spots for SRR13695456.sra Read 978638 spots for SRR13695456.sra Written 978638 spots for SRR13695456.sra Read 978638 spots for SRR13695456.sra Written 978638 spots for SRR13695456.sra Read 978638 spots for SRR13695456.sra Written 978638 spots for SRR13695456.sra Read 978638 spots for SRR13695456.sra Written 978638 spots for SRR13695456.sra Read 978638 spots for SRR13695456.sra Written 978638 spots for SRR13695456.sra Read 978638 spots for SRR13695456.sra Written 978638 spots for SRR13695456.sra Read 978638 spots for SRR13695456.sra Written 978638 spots for SRR13695456.sra Read 978638 spots for SRR13695456.sra Written 978638 spots for SRR13695456.sra Read 978638 spots for SRR13695456.sra Written 978638 spots for SRR13695456.sra Read 978638 spots for SRR13695456.sra Written 978638 spots for SRR13695456.sra Read 978638 spots for SRR13695456.sra Written 978638 spots for SRR13695456.sra Read 978638 spots for SRR13695456.sra Written 978638 spots for SRR13695456.sra Read 978638 spots for SRR13695456.sra Written 978638 spots for SRR13695456.sra Read 978638 spots for SRR13695456.sra Written 978638 spots for SRR13695456.sra Read 978638 spots for SRR13695456.sra Written 978638 spots for SRR13695456.sra Read 978638 spots for SRR13695456.sra Written 978638 spots for SRR13695456.sra Read 978654 spots for SRR13695456.sra Written 978654 spots for SRR13695456.sra Read 978638 spots for SRR13695456.sra Written 978638 spots for SRR13695456.sra Read 978638 spots for SRR13695456.sra Written 978638 spots for SRR13695456.sra SRR ids: ['SRR13695456.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_h4i9d6wa SRR13695456.sra spots: 19572776 blocks: [[1, 978638], [978639, 1957276], [1957277, 2935914], [2935915, 3914552], [3914553, 4893190], [4893191, 5871828], [5871829, 6850466], [6850467, 7829104], [7829105, 8807742], [8807743, 9786380], [9786381, 10765018], [10765019, 11743656], [11743657, 12722294], [12722295, 13700932], [13700933, 14679570], [14679571, 15658208], [15658209, 16636846], [16636847, 17615484], [17615485, 18594122], [18594123, 19572776]] SRR13695456 file size 6629985 SRR13695456 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695456 SRR13695456_1.fastq SRR13695456_2.fastq Input file: SRR13695456_1.fastq Paired file: SRR13695456_2.fastq trimmed: SRR13695456-trimmed-pair1.fastq, SRR13695456-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 04:28:53 2025 >> started Wed Feb 12 04:29:17 2025 >> done (23.675s) 19572776 read pairs processed; of these: 142 ( 0.00%) short read pairs filtered out after trimming by size control 718 ( 0.00%) empty read pairs filtered out after trimming by size control 19571916 (100.00%) read pairs available; of these: 2498469 (12.77%) trimmed read pairs available after processing 17073447 (87.23%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 19 2 0.00% 20 3 0.00% 21 2 0.00% 22 3 0.00% 23 4 0.00% 24 2 0.00% 25 4 0.00% 26 6 0.00% 27 3 0.00% 28 1 0.00% 29 6 0.00% 30 6 0.00% 31 5 0.00% 32 0 0.00% 33 4 0.00% 34 7 0.00% 35 3 0.00% 36 10 0.00% 37 4 0.00% 38 8 0.00% 39 14 0.00% 40 9 0.00% 41 25 0.00% 42 29 0.00% 43 33 0.00% 44 29 0.00% 45 34 0.00% 46 26 0.00% 47 51 0.00% 48 70 0.00% 49 74 0.00% 50 81 0.00% 51 98 0.00% 52 106 0.00% 53 110 0.00% 54 133 0.00% 55 150 0.00% 56 167 0.00% 57 204 0.00% 58 243 0.00% 59 290 0.00% 60 352 0.00% 61 356 0.00% 62 394 0.00% 63 537 0.00% 64 529 0.00% 65 643 0.00% 66 688 0.00% 67 801 0.00% 68 905 0.00% 69 1020 0.01% 70 1238 0.01% 71 1324 0.01% 72 1655 0.01% 73 1792 0.01% 74 2122 0.01% 75 2459 0.01% 76 2554 0.01% 77 2868 0.01% 78 3286 0.02% 79 3539 0.02% 80 3969 0.02% 81 4525 0.02% 82 4979 0.03% 83 5547 0.03% 84 6574 0.03% 85 6961 0.04% 86 7281 0.04% 87 8215 0.04% 88 9062 0.05% 89 9086 0.05% 90 10169 0.05% 91 10926 0.06% 92 11549 0.06% 93 12723 0.07% 94 13763 0.07% 95 14674 0.07% 96 15460 0.08% 97 16604 0.08% 98 16874 0.09% 99 17929 0.09% 100 19015 0.10% 101 19804 0.10% 102 20852 0.11% 103 21559 0.11% 104 23118 0.12% 105 23951 0.12% 106 24957 0.13% 107 26456 0.14% 108 27354 0.14% 109 28213 0.14% 110 29003 0.15% 111 30179 0.15% 112 30634 0.16% 113 31602 0.16% 114 33074 0.17% 115 34828 0.18% 116 35692 0.18% 117 36732 0.19% 118 37925 0.19% 119 38133 0.19% 120 39693 0.20% 121 40874 0.21% 122 41727 0.21% 123 42298 0.22% 124 44155 0.23% 125 44855 0.23% 126 45970 0.23% 127 46869 0.24% 128 48101 0.25% 129 48637 0.25% 130 50692 0.26% 131 50643 0.26% 132 51730 0.26% 133 52599 0.27% 134 53115 0.27% 135 54224 0.28% 136 55229 0.28% 137 56674 0.29% 138 57138 0.29% 139 58894 0.30% 140 60272 0.31% 141 60079 0.31% 142 61273 0.31% 143 61462 0.31% 144 63186 0.32% 145 63545 0.32% 146 64758 0.33% 147 65706 0.34% 148 67442 0.34% 149 67161 0.34% 150 68396 0.35% 151 17073447 87.23% 19571916 reads passed initial QC criterion=sequence-density sequence-density=0.27 sequence-density-rank=1 fanout-score=2.04 fanout-score-rank=22 prefix-density=0.28 prefix-fanout=2.0 sequence=CGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTT criterion=fanout-score sequence-density=0.01 sequence-density-rank=26 fanout-score=349.26 fanout-score-rank=1 prefix-density=0.28 prefix-fanout=17.7 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGTTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGAGTATGAATGTGTTCTCTGTG criterion=sequence-density sequence-density=0.43 sequence-density-rank=1 fanout-score=6.14 fanout-score-rank=12 prefix-density=1.57 prefix-fanout=1.7 sequence=CACCTGCGACACCTGCGACTGCG criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=36.40 fanout-score-rank=1 prefix-density=0.08 prefix-fanout=3.1 sequence=CAATATTTTTCAGTATGAAAGCTTTAGTGATAGCTATTCTTATAGCTACCATTGCCTTCTCTCCCTTATCCATGGCAGCTCGAGAATTGGTCGACTATGGAAAAGATAGCGTTACCGTCAATATCCCATCAACTGGCGATGTATCATCTAGAAGCCAGCCTCCTACCTATGCCCCACGAACTGGCAGTGGAT SRR13695456 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 04:30:14 Started mapping on | Feb 12 04:30:14 Finished on | Feb 12 04:32:41 Mapping speed, Million of reads per hour | 479.31 Number of input reads | 19571916 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 18248850 Uniquely mapped reads % | 93.24% Average mapped length | 294.17 Number of splices: Total | 17729142 Number of splices: Annotated (sjdb) | 17322371 Number of splices: GT/AG | 17374345 Number of splices: GC/AG | 266271 Number of splices: AT/AC | 10823 Number of splices: Non-canonical | 77703 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 2.84 Insertion rate per base | 0.02% Insertion average length | 2.38 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 439173 % of reads mapped to multiple loci | 2.24% Number of reads mapped to too many loci | 50778 % of reads mapped to too many loci | 0.26% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.17% % of reads unmapped: other | 0.08% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 884114 884114 884114 N_multimapping 439173 439173 439173 N_noFeature 766123 17720596 1093653 N_ambiguous 323689 2304 121223 UnstrandedReadsAssigned:17159038 PositiveStrandReadsAssigned:525950 NegativeStrandReadsAssigned:17033974 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695456 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695456-trimmed-pair1.fastq SRR13695456-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 19,571,916 reads, 16,960,886 reads pseudoaligned [quant] estimated average fragment length: 247.513 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,076 rounds 52401 SRR13695456.ke.tsv 34699 SRR13695456.se.tsv 87100 total ==> SRR13695456.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1771.49 851 23.9074 Potri.005G024800.1.v4.1 1035 788.487 395 24.9312 Potri.004G059700.1.v4.1 961 714.567 0 0 Potri.007G009000.2.v4.1 1416 1169.49 0 0 Potri.003G141000.2.v4.1 2943 2696.49 862 15.9092 Potri.016G087400.1.v4.1 270 86.369 931 536.454 Potri.015G069301.1.v4.1 564 327.241 0 0 Potri.010G195200.1.v4.1 1773 1526.49 254.899 8.31029 Potri.012G127500.1.v4.1 977 730.552 131 8.92402 ==> SRR13695456.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 90 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 297 Potri.001G212900.v4.1 3 Potri.001G182400.v4.1 2 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 15 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 4 SRR13695456 completed mapping pipeline successfully