Starting /dee2/code/volunteer_pipeline.sh SRR13695457 current disk space = 3049210200064 free memory = 1579380572 SRR13695457 SRAfilesize 2e0c45701cae32ca39640a08d8beccc6 SRR13695457.sra SRR13695457.sra file validated SRR13695457 is paired end SRR13695457 is conventional basespace SRR13695457 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695457_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.481 37.0 37.0 37.0 37.0 37.0 2 36.41575 37.0 37.0 37.0 37.0 37.0 3 36.5895 37.0 37.0 37.0 37.0 37.0 4 36.641 37.0 37.0 37.0 37.0 37.0 5 36.5545 37.0 37.0 37.0 37.0 37.0 6 36.5965 37.0 37.0 37.0 37.0 37.0 7 36.489 37.0 37.0 37.0 37.0 37.0 8 36.6155 37.0 37.0 37.0 37.0 37.0 9 36.5235 37.0 37.0 37.0 37.0 37.0 10-14 36.5645 37.0 37.0 37.0 37.0 37.0 15-19 36.5436 37.0 37.0 37.0 37.0 37.0 20-24 36.490500000000004 37.0 37.0 37.0 37.0 37.0 25-29 36.4514 37.0 37.0 37.0 37.0 37.0 30-34 36.4272 37.0 37.0 37.0 37.0 37.0 35-39 36.384899999999995 37.0 37.0 37.0 37.0 37.0 40-44 36.4301 37.0 37.0 37.0 37.0 37.0 45-49 36.348 37.0 37.0 37.0 37.0 37.0 50-54 36.375299999999996 37.0 37.0 37.0 37.0 37.0 55-59 36.426700000000004 37.0 37.0 37.0 37.0 37.0 60-64 36.404399999999995 37.0 37.0 37.0 37.0 37.0 65-69 36.2769 37.0 37.0 37.0 37.0 37.0 70-74 36.3159 37.0 37.0 37.0 37.0 37.0 75-79 36.2746 37.0 37.0 37.0 37.0 37.0 80-84 36.2445 37.0 37.0 37.0 37.0 37.0 85-89 36.1942 37.0 37.0 37.0 37.0 37.0 90-94 36.1745 37.0 37.0 37.0 37.0 37.0 95-99 36.1183 37.0 37.0 37.0 37.0 37.0 100-104 36.2068 37.0 37.0 37.0 37.0 37.0 105-109 36.080400000000004 37.0 37.0 37.0 37.0 37.0 110-114 36.17059999999999 37.0 37.0 37.0 37.0 37.0 115-119 36.0512 37.0 37.0 37.0 37.0 37.0 120-124 35.9423 37.0 37.0 37.0 37.0 37.0 125-129 35.962599999999995 37.0 37.0 37.0 37.0 37.0 130-134 35.925700000000006 37.0 37.0 37.0 37.0 37.0 135-139 35.8441 37.0 37.0 37.0 37.0 37.0 140-144 35.84009999999999 37.0 37.0 37.0 37.0 37.0 145-149 35.6228 37.0 37.0 37.0 37.0 37.0 150-151 35.435 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 1.0 24 1.0 25 1.0 26 2.0 27 4.0 28 12.0 29 23.0 30 34.0 31 40.0 32 55.0 33 61.0 34 136.0 35 322.0 36 2982.0 37 326.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.275 10.4 7.2749999999999995 51.05 2 17.313956401904285 13.856176396893009 42.671009771986974 26.158857429215736 3 18.575 16.05 26.825 38.550000000000004 4 23.525 23.549999999999997 22.15 30.775000000000002 5 22.900000000000002 31.674999999999997 24.825 20.599999999999998 6 19.525000000000002 35.825 23.525 21.125 7 14.799999999999999 27.125 41.575 16.5 8 17.925 26.650000000000002 32.074999999999996 23.35 9 17.275 24.5 35.75 22.475 10-14 19.3 30.37 27.845 22.485 15-19 20.585 28.865000000000002 27.639999999999997 22.91 20-24 19.805 28.985 27.589999999999996 23.62 25-29 19.665 29.17 28.24 22.925 30-34 19.82 28.51 28.1 23.57 35-39 19.85 27.97 28.000000000000004 24.18 40-44 19.675 29.054999999999996 28.475 22.795 45-49 19.81 28.95 27.32 23.919999999999998 50-54 20.515 29.09 27.095000000000002 23.3 55-59 19.85 28.46 28.17 23.52 60-64 19.77 29.099999999999998 27.785 23.345 65-69 20.27 28.349999999999998 28.28 23.1 70-74 20.294999999999998 28.68 27.485 23.54 75-79 19.67 29.509999999999998 27.139999999999997 23.68 80-84 19.93 28.585 27.075 24.41 85-89 19.915 29.415000000000003 27.6 23.07 90-94 20.815 28.43 27.37 23.385 95-99 20.419999999999998 28.455000000000002 28.360000000000003 22.765 100-104 20.205000000000002 29.065 26.93 23.799999999999997 105-109 20.674999999999997 28.845 26.915 23.565 110-114 20.595 28.03 28.62 22.755 115-119 20.36 29.525000000000002 26.634999999999998 23.48 120-124 21.295 29.42 26.705000000000002 22.58 125-129 20.794999999999998 28.335 27.700000000000003 23.169999999999998 130-134 21.235 28.744999999999997 26.669999999999998 23.35 135-139 21.265 28.915000000000003 26.43 23.39 140-144 20.205000000000002 29.885 25.790000000000003 24.12 145-149 19.695 28.360000000000003 27.045 24.9 150-151 21.8625 27.9375 27.250000000000004 22.95 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 1.0 24 2.0 25 3.0 26 7.0 27 12.5 28 12.0 29 11.5 30 16.5 31 16.0 32 27.0 33 46.0 34 65.0 35 83.0 36 90.5 37 108.0 38 136.5 39 155.0 40 187.5 41 216.5 42 240.5 43 299.5 44 319.0 45 283.5 46 260.5 47 254.5 48 235.0 49 197.0 50 161.0 51 130.5 52 106.5 53 82.0 54 61.0 55 47.5 56 34.0 57 21.5 58 18.5 59 16.0 60 11.5 61 13.0 62 6.0 63 1.0 64 1.0 65 0.0 66 0.5 67 0.5 68 0.0 69 1.0 70 1.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.22499999999999998 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.875 #Duplication Level Percentage of deduplicated Percentage of total 1 71.59212880143113 50.025 2 19.105545617173526 26.700000000000003 3 6.1180679785330945 12.825000000000001 4 1.7889087656529516 5.0 5 0.8228980322003578 2.875 6 0.3935599284436494 1.6500000000000001 7 0.1073345259391771 0.525 8 0.07155635062611806 0.4 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTCTGGGTTTCACTCAAAGAAAAGAAAGTTTTGTTGGGTTGGTTTGGAC 8 0.2 No Hit CATTTCACAATCTTCTCGGCCTCTTGACTCATCTTCTCTTGCTCTTTCAT 8 0.2 No Hit TTCATTTCTCTAGAATCAGCTCTAGTCTGTTGGGCTCTGATCACAGGCAA 7 0.17500000000000002 No Hit CACGTAAGTTTTGAGTGAGGAGGGCGTCCTTGGCATAATCATTCTCTGCT 7 0.17500000000000002 No Hit GTTTCCTTTCTCGGTGATAACATGAATAAGAACAGGTCCTGGTGCTGGCA 7 0.17500000000000002 No Hit CTGCAATGGAGTCATATAGATCCTGAGTAGCATGACTGTGATTTGTGCCT 6 0.15 No Hit GCATGTATAAGGTCACCTAGCTAACACAATCTTCTCTCTAATCAATTTCA 6 0.15 No Hit GCTGGCAAAACCTTAGAGGTTATGTACTCTTCAAATATCTTCTTATAATA 6 0.15 No Hit CTGGTTTCAAGATATTAGCAGCATCAATAGCTCCTTCTGCAGCTCCTGCC 6 0.15 No Hit GTGTAATACTCTGTTGTATATCCAATCACAAGTCCAGCCCACAAGCCAAT 6 0.15 No Hit CTTGGCGGCCGTAGGGGCTCCATCGATATAAAAATTCCAACTGATAGTAC 6 0.15 No Hit AGAAAACAAACGGAAAAGTTCGCAAAAGCAACCACAAACATGTTAAGACA 6 0.15 No Hit ATGGCCATTAACTCTGGAATCCCCTAACTGCCTTGCCACTCTTCTTGTTA 6 0.15 No Hit GTACATAATATTACTTGGAAGAGCTGCTGGAAGAGGATGGCCTTCCCTGT 6 0.15 No Hit CACCCTTCAGTCCTAGAGTTTTGTTCACCAAGCCATCTCTCACAAACGGG 6 0.15 No Hit CTCCTCAAGCTCAAGCAACACTTGAGATGCCTCAGTGCATCCAAACATGG 6 0.15 No Hit CCTCCTTCAGTTTGTATAATGTCAACTCCTTCTTGTTCTAATAGCTCTGC 5 0.125 No Hit TGCACCCGAGGTCTACACTAGCCAGCAACATTTTAGACAATGGTGGATCC 5 0.125 No Hit CAATTGCATCTTCAGCAGCGTTCATGTCGAGTGCTTTCATTCCAGGAACA 5 0.125 No Hit CCCAATAAAGAACTTTCCATAGATCTCCAAATTCAAGCACACTCTTTTAC 5 0.125 No Hit CCACTCCCTGAAGAACTCCCTGAATCAGAATTTGAAGATGAAGAATCATC 5 0.125 No Hit CTGAACTCTCATGGACTAACATCCTGAAATCTTATGATATTATGGAAGTG 5 0.125 No Hit CTGCAAATCTACTCCTCCAATTTTGAGAACTGCAAGCACTGAACCCATAC 5 0.125 No Hit GCTTGACATCTCTATGCATTATTCCCTGTGAGTGGCAGTAATCTAATGCC 5 0.125 No Hit CATTCCACCACTGAAGGGCTTCAAAAATTGCTTCACGGAAGAACCAGACT 5 0.125 No Hit GTCGTCGTCGTCGTCGTCATCATCTTGTTCATCGCCATTTTCCTCTTCAT 5 0.125 No Hit GCCCTTGCAACCATCGATGAATGATAGGTGGTGCTTCTCTGTTTCCTGTA 5 0.125 No Hit CCTCGGTCCAGTGCCCAGGATTTATCACATGGTCGTACACAATAGAGAGA 5 0.125 No Hit CAGAAATGAACCTCCAAAACCAGTAGTAGAAGAGCCCATTGTTTCAGAAA 5 0.125 No Hit TGAAGATTCTTGAAAGATCTGGTGTTGTTTTGGATTTTCTTGTCCCATGT 5 0.125 No Hit GCCAAATCCCTTCACGGGTTCGATTGCATCGGCGACATGAGCATAGTCTT 5 0.125 No Hit GTTCACCATCTTTCGGGTCCCGACAGGCATGCTCTCACTCGAACCCTTCT 5 0.125 No Hit CTTATATTTCCCAGCAAGGTTAGTCATGTAATGATGCACCCTGTTGAAGT 5 0.125 No Hit CTGCAATCTTCTTCTTCAATACTTCAATGTCCGTGGCTAGTTCTTTTCTG 5 0.125 No Hit CTTGAAAATTCCAATGCAGACACCAATCCTATCTTCTCTGGTAGGAGCCC 5 0.125 No Hit TCCTCGTCACGATCAAAACTCAAAGTACAAACTACATTTGTGGAGCTCAT 5 0.125 No Hit CAATGAGGAAACATACCTTGAAGGTGTGTATGATACAGGAGATGAAGAGT 5 0.125 No Hit TCTTGATTATATCAGCCGCCTCACGGTACCTTTGCCTGATAAGTTTTGCG 5 0.125 No Hit CACCACTGCTTTCGGGGAGGAAGACTTAGGAAGCCAACTCTTTGTGAAAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.037500000000000006 0.0 0.0 0.0 0.0 74-75 0.1125 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.1875 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.2375 0.0 0.0 0.0 0.0 84-85 0.35 0.0 0.0 0.0 0.0 86-87 0.475 0.0 0.0 0.0 0.0 88-89 0.6000000000000001 0.0 0.0 0.0 0.0 90-91 0.8375 0.0 0.0 0.0 0.0 92-93 0.925 0.0 0.0 0.0 0.0 94-95 1.0 0.0 0.0 0.0 0.0 96-97 1.2125 0.0 0.0 0.0 0.0 98-99 1.2999999999999998 0.0 0.0 0.0 0.0 100-101 1.4500000000000002 0.0 0.0 0.0 0.0 102-103 1.575 0.0 0.0 0.0 0.0 104-105 1.9249999999999998 0.0 0.0 0.0 0.0 106-107 2.25 0.0 0.0 0.0 0.0 108-109 2.575 0.0 0.0 0.0 0.0 110-111 2.8625 0.0 0.0 0.0 0.0 112-113 3.1125 0.0 0.0 0.0 0.0 114-115 3.35 0.0 0.0 0.0 0.0 116-117 3.8125 0.0 0.0 0.0 0.0 118-119 4.375 0.0 0.0 0.0 0.0 120-121 4.9625 0.0 0.0 0.0 0.0 122-123 5.7 0.0 0.0 0.0 0.0 124-125 6.2125 0.0 0.0 0.0 0.0 126-127 6.762499999999999 0.0 0.0 0.0 0.0 128-129 7.5 0.0 0.0 0.0 0.0 130-131 7.925 0.0 0.0 0.0 0.0 132-133 8.787500000000001 0.0 0.0 0.0 0.0 134-135 9.4375 0.0 0.0 0.0 0.0 136-137 9.95 0.0 0.0 0.0 0.0 138-139 10.6625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATGAAG 10 0.006830828 145.0 5 CTAACTC 10 0.006830828 145.0 1 >>END_MODULE SRR13695457 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695457_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.1865 37.0 37.0 37.0 37.0 37.0 2 36.2145 37.0 37.0 37.0 37.0 37.0 3 36.211 37.0 37.0 37.0 37.0 37.0 4 36.2785 37.0 37.0 37.0 37.0 37.0 5 36.365 37.0 37.0 37.0 37.0 37.0 6 36.267 37.0 37.0 37.0 37.0 37.0 7 36.405 37.0 37.0 37.0 37.0 37.0 8 36.355 37.0 37.0 37.0 37.0 37.0 9 36.331 37.0 37.0 37.0 37.0 37.0 10-14 36.311299999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.3105 37.0 37.0 37.0 37.0 37.0 20-24 36.2887 37.0 37.0 37.0 37.0 37.0 25-29 36.19015 37.0 37.0 37.0 37.0 37.0 30-34 36.21005 37.0 37.0 37.0 37.0 37.0 35-39 36.204449999999994 37.0 37.0 37.0 37.0 37.0 40-44 36.156349999999996 37.0 37.0 37.0 37.0 37.0 45-49 36.12284999999999 37.0 37.0 37.0 37.0 37.0 50-54 36.129949999999994 37.0 37.0 37.0 37.0 37.0 55-59 36.07695 37.0 37.0 37.0 37.0 37.0 60-64 36.017250000000004 37.0 37.0 37.0 37.0 37.0 65-69 35.9998 37.0 37.0 37.0 37.0 37.0 70-74 36.02015 37.0 37.0 37.0 37.0 37.0 75-79 35.98035 37.0 37.0 37.0 37.0 37.0 80-84 36.00345 37.0 37.0 37.0 37.0 37.0 85-89 35.87545 37.0 37.0 37.0 37.0 37.0 90-94 35.845150000000004 37.0 37.0 37.0 37.0 37.0 95-99 35.85355 37.0 37.0 37.0 37.0 37.0 100-104 35.850049999999996 37.0 37.0 37.0 37.0 37.0 105-109 35.86245 37.0 37.0 37.0 37.0 37.0 110-114 35.77935 37.0 37.0 37.0 37.0 37.0 115-119 35.70575 37.0 37.0 37.0 37.0 37.0 120-124 35.695949999999996 37.0 37.0 37.0 37.0 37.0 125-129 35.652049999999996 37.0 37.0 37.0 37.0 37.0 130-134 35.543549999999996 37.0 37.0 37.0 37.0 37.0 135-139 35.5168 37.0 37.0 37.0 37.0 37.0 140-144 35.351350000000004 37.0 37.0 37.0 34.6 37.0 145-149 35.2879 37.0 37.0 37.0 29.8 37.0 150-151 35.014375 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.0 12 0.0 13 1.0 14 0.0 15 0.0 16 1.0 17 0.0 18 0.0 19 2.0 20 1.0 21 0.0 22 3.0 23 1.0 24 7.0 25 6.0 26 6.0 27 10.0 28 13.0 29 13.0 30 21.0 31 38.0 32 66.0 33 113.0 34 196.0 35 602.0 36 2708.0 37 190.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 32.27341011517276 21.457185778668002 11.667501251877816 34.60190285428143 2 25.674999999999997 27.150000000000002 32.375 14.799999999999999 3 18.4 26.775 33.625 21.2 4 24.125 33.175 23.625 19.075 5 24.375 35.75 23.724999999999998 16.150000000000002 6 19.5 39.95 23.5 17.05 7 18.6 22.125 39.975 19.3 8 20.45 25.75 29.7 24.099999999999998 9 21.224999999999998 23.425 31.900000000000002 23.45 10-14 21.845 29.825000000000003 27.544999999999998 20.785 15-19 22.445 27.575 28.425 21.555 20-24 22.854570914182837 28.710742148429684 28.005601120224043 20.429085817163433 25-29 21.870467616904225 29.072268067016754 28.037009252313077 21.02025506376594 30-34 22.52563140785196 28.347086771692926 28.732183045761438 20.395098774693672 35-39 23.225806451612904 28.037009252313077 27.711927981995498 21.02525631407852 40-44 22.435608902225557 28.192048012003003 28.597149287321834 20.775193798449614 45-49 22.360590147536886 28.667166791697923 28.27706926731683 20.69517379344836 50-54 22.58064516129032 27.901975493873472 28.967241810452617 20.550137534383595 55-59 22.780695173793447 28.217054263565895 27.89197299324831 21.11027756939235 60-64 22.945736434108525 27.53188297074269 29.08227056764191 20.44011002750688 65-69 23.05730573057306 27.83778377837784 28.72787278727873 20.377037703770377 70-74 23.210802700675167 28.067016754188543 27.71692923230808 21.005251312828207 75-79 22.035508877219304 28.322080520130033 28.3520880220055 21.29032258064516 80-84 23.23580895223806 27.93198299574894 28.48712178044511 20.34508627156789 85-89 23.865966491622906 28.872218054513628 27.236809202300577 20.02500625156289 90-94 23.980995248812203 27.60190047511878 27.646911727931982 20.770192548137032 95-99 23.225806451612904 27.751937984496124 28.767191797949486 20.255063765941486 100-104 23.755938984746187 28.512128032008 27.58689672418104 20.145036259064767 105-109 23.850962740685173 27.731932983245812 28.27706926731683 20.14003500875219 110-114 23.760940235058765 27.6419104776194 28.632158039509875 19.964991247811952 115-119 23.970992748187047 28.40710177544386 27.526881720430108 20.095023755938985 120-124 23.570892723180794 28.322080520130033 27.41185296324081 20.69517379344836 125-129 25.07126781695424 28.30207551887972 27.58689672418104 19.039759939984997 130-134 25.436446400880396 28.23770696813566 27.22225001250563 19.103596618478317 135-139 25.237571271381416 27.793338001400418 27.613283985195558 19.355806742022608 140-144 25.646411602900727 27.461865466366593 27.506876719179797 19.38484621155289 145-149 26.823046914074222 26.998099429828947 26.657997399219767 19.520856256877064 150-151 26.941838649155724 26.79174484052533 27.629768605378363 18.636647904940588 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 0.0 10 0.5 11 1.0 12 1.0 13 0.5 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.0 20 0.5 21 1.0 22 1.0 23 1.5 24 3.0 25 3.0 26 4.0 27 6.5 28 6.0 29 10.5 30 23.0 31 27.5 32 36.5 33 48.0 34 57.0 35 76.5 36 97.5 37 110.0 38 145.5 39 191.5 40 207.0 41 241.5 42 275.5 43 285.0 44 288.0 45 263.5 46 237.5 47 228.0 48 220.5 49 192.5 50 154.5 51 126.5 52 111.5 53 87.0 54 52.5 55 37.5 56 32.0 57 31.5 58 24.0 59 14.5 60 11.5 61 6.5 62 4.5 63 6.0 64 4.5 65 1.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.02 25-29 0.025 30-34 0.025 35-39 0.025 40-44 0.025 45-49 0.025 50-54 0.025 55-59 0.025 60-64 0.025 65-69 0.01 70-74 0.025 75-79 0.025 80-84 0.025 85-89 0.025 90-94 0.025 95-99 0.025 100-104 0.025 105-109 0.025 110-114 0.025 115-119 0.025 120-124 0.025 125-129 0.025 130-134 0.045 135-139 0.03 140-144 0.025 145-149 0.03 150-151 0.0625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.39999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 72.19460227272727 50.824999999999996 2 18.75 26.400000000000002 3 5.823863636363636 12.3 4 1.9176136363636365 5.4 5 0.8522727272727272 3.0 6 0.31960227272727276 1.35 7 0.1065340909090909 0.525 8 0.03551136363636364 0.2 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGTCTTTACACGGGTCTGATCATTCTAACTCATGGGTGGAAGATGTGATT 8 0.2 No Hit GGCAAACACAGGAAATTGCAGCTAAAGTGGATTCCTATATGAGAGGAGTG 7 0.17500000000000002 No Hit ACCAAAAAAAAAAAGAGGCTCAGTAGCAGAATGAAGAAAGCTGAGCTAGT 7 0.17500000000000002 No Hit CATGCAACTATGCTATCTCAGGCACTGGATCATCTGAGCTTAATGCCAAG 7 0.17500000000000002 No Hit AAAGGTTGGGTCTACCGTGAGCACCACAGCTCACCAGGGTACTATGATGG 6 0.15 No Hit CAGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 6 0.15 No Hit TAAAAAAACACTGTAAAGTCACAGTCTCTCCTGTTGATAGGATCTGGAAC 6 0.15 No Hit CGCATGGTGGGCGAGAGCACGGAGAACCTGGCTGGATCCACTGTTGGACC 6 0.15 No Hit GACAAAAACGCATAAGAGAGGGACAAAGGGAAGTCAGGGAAAAATTTCAA 6 0.15 No Hit TGGATCCACCTGCTGGGTGGTTATTAACAAAAACTAATGATTGATATCTC 6 0.15 No Hit AACAATTCTCATGACTGCTGGGATTGCCATGGTCAGCTTCTTTGCTTTGC 6 0.15 No Hit CACCTACATGTTGATCAACAAGGCTGGAAAAGCCCATTATGTGAAATTTC 6 0.15 No Hit CATATCTTCCTTCACATCCTGTGGTGCCTACAGGTGGTATTCCAGCCCCT 6 0.15 No Hit GGAAGGGGAAAGTACAGTGAGGTTTTTGAGGGCATAAATGTCAATAGCAA 5 0.125 No Hit TGGTTTCTTTCAGCATCATGCGTTCTTTATTTGATTACTCATTTTTCTCT 5 0.125 No Hit CTGGCATTTCAGTTGGAGTTGGAAAATCTTCTGATAGCACACAGCTTTCA 5 0.125 No Hit GGAGTCTGACATGTGTGCGAGTCAACGGGCGAGTAAACCCGTAAGGCGCA 5 0.125 No Hit GATAGATATGCAGAGGCGGTCTCTCTCTCTCTCTCTCTCTATCTGGCTCT 5 0.125 No Hit TGGGGATGAAGATGTTGACATTGGCGATGAGATGCCAATGAGTAGTTTCC 5 0.125 No Hit ATTAAAACTTCTGCAAGTGGGTTTTCTCTGTAACCCCCAAATCTTACTTC 5 0.125 No Hit GAAGAAATCTGCAATCGTATTACCTGGTTCAAAGGTGTTGAAGCACATAG 5 0.125 No Hit CTGGGATCTAAATATTTGGTTGTCTGGCATGCCCCTGAGGTCGTTTTGCA 5 0.125 No Hit CTGCTTTAAATTTTCCTCATTTCGCTGCTAAAAATGTCTCTTAGTAGTAG 5 0.125 No Hit GGAGACTAAAAGGATTCTTCCATCCGTGACATTATCAGTCACTGTTCCTC 5 0.125 No Hit TGTGCTCCGCAATGTTGCGAATATGGTCCCGCCATACGATAAGACTAAGT 5 0.125 No Hit GGTGTGTATCAATCAAATCCTGCAGGTAGCGAGGATTGAACTTATTCCTG 5 0.125 No Hit CCTACTTTCAACTTTTCTGTCGGGCCTTCACCAAATCTGAGTGCAGCAGT 5 0.125 No Hit CTTGAACTAAATGCTATCTAATTAACTATTGCTTTGCGGTTCTGCTAAGT 5 0.125 No Hit CAGGTTTGCTCCACTCCAGATCAGAGCCTCTTCTTCAGAAGAATCCTCTG 5 0.125 No Hit CAATATTATGACAGGCTGACAGCATCCATTTTCAGTGATCTCTCCTGTCT 5 0.125 No Hit CAATCATCTTCCTTGTTTCAGATACTGTTCAGCTCCAACATGGCCTCCAT 5 0.125 No Hit CTGGTCTTTGCCAAGATGGGAATTAGCCCAATCATGATGAGTGCCGGAGA 5 0.125 No Hit GGAAAATGTTATCGTCTCTACACAGAAAGTGCATACCGCAATGAGATGTC 5 0.125 No Hit CAGCAGAGGATTTAATAGTGAAAGGAGGGAGAGTTTCTGGTGTGGTTACT 5 0.125 No Hit CCCATTCGCAAAGGAGGAGCTTGCATCCCTCCTCCTCCAAACTTACAGGT 5 0.125 No Hit CGTGAGGCGGCTGATATAATCAAGAAGAAGGGAAAGATGTGCTGCCTCTT 5 0.125 No Hit TGTGAAGTCAGGGTATTCTATTACATTTAACTTCAAAGAGAATCCTCATT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.037500000000000006 0.0 0.0 0.0 0.0 74-75 0.1125 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.1875 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.2375 0.0 0.0 0.0 0.0 84-85 0.35 0.0 0.0 0.0 0.0 86-87 0.475 0.0 0.0 0.0 0.0 88-89 0.6000000000000001 0.0 0.0 0.0 0.0 90-91 0.8375 0.0 0.0 0.0 0.0 92-93 0.925 0.0 0.0 0.0 0.0 94-95 1.0 0.0 0.0 0.0 0.0 96-97 1.2125 0.0 0.0 0.0 0.0 98-99 1.2999999999999998 0.0 0.0 0.0 0.0 100-101 1.4500000000000002 0.0 0.0 0.0 0.0 102-103 1.575 0.0 0.0 0.0 0.0 104-105 1.9249999999999998 0.0 0.0 0.0 0.0 106-107 2.25 0.0 0.0 0.0 0.0 108-109 2.575 0.0 0.0 0.0 0.0 110-111 2.8625 0.0 0.0 0.0 0.0 112-113 3.1125 0.0 0.0 0.0 0.0 114-115 3.3375000000000004 0.0 0.0 0.0 0.0 116-117 3.8125 0.0 0.0 0.0 0.0 118-119 4.375 0.0 0.0 0.0 0.0 120-121 4.987500000000001 0.0 0.0 0.0 0.0 122-123 5.7125 0.0 0.0 0.0 0.0 124-125 6.1875 0.0 0.0 0.0 0.0 126-127 6.725 0.0 0.0 0.0 0.0 128-129 7.475 0.0 0.0 0.0 0.0 130-131 7.925 0.0 0.0 0.0 0.0 132-133 8.850000000000001 0.0 0.0 0.0 0.0 134-135 9.525 0.0 0.0 0.0 0.0 136-137 10.037500000000001 0.0 0.0 0.0 0.0 138-139 10.8 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTTT 10 0.006830828 145.0 8 GATGTTC 10 0.006830828 145.0 5 GATGTAC 10 0.006830828 145.0 5 >>END_MODULE Read 752248 spots for SRR13695457.sra Written 752248 spots for SRR13695457.sra Read 752248 spots for SRR13695457.sra Written 752248 spots for SRR13695457.sra Read 752248 spots for SRR13695457.sra Written 752248 spots for SRR13695457.sra Read 752248 spots for SRR13695457.sra Written 752248 spots for SRR13695457.sra Read 752248 spots for SRR13695457.sra Written 752248 spots for SRR13695457.sra Read 752248 spots for SRR13695457.sra Written 752248 spots for SRR13695457.sra Read 752267 spots for SRR13695457.sra Written 752267 spots for SRR13695457.sra Read 752248 spots for SRR13695457.sra Written 752248 spots for SRR13695457.sra Read 752248 spots for SRR13695457.sra Written 752248 spots for SRR13695457.sra Read 752248 spots for SRR13695457.sra Written 752248 spots for SRR13695457.sra Read 752248 spots for SRR13695457.sra Written 752248 spots for SRR13695457.sra Read 752248 spots for SRR13695457.sra Written 752248 spots for SRR13695457.sra Read 752248 spots for SRR13695457.sra Written 752248 spots for SRR13695457.sra Read 752248 spots for SRR13695457.sra Written 752248 spots for SRR13695457.sra Read 752248 spots for SRR13695457.sra Written 752248 spots for SRR13695457.sra Read 752248 spots for SRR13695457.sra Written 752248 spots for SRR13695457.sra Read 752248 spots for SRR13695457.sra Written 752248 spots for SRR13695457.sra Read 752248 spots for SRR13695457.sra Written 752248 spots for SRR13695457.sra Read 752248 spots for SRR13695457.sra Written 752248 spots for SRR13695457.sra Read 752248 spots for SRR13695457.sra Written 752248 spots for SRR13695457.sra SRR ids: ['SRR13695457.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_eqjhm8yx SRR13695457.sra spots: 15044979 blocks: [[1, 752248], [752249, 1504496], [1504497, 2256744], [2256745, 3008992], [3008993, 3761240], [3761241, 4513488], [4513489, 5265736], [5265737, 6017984], [6017985, 6770232], [6770233, 7522480], [7522481, 8274728], [8274729, 9026976], [9026977, 9779224], [9779225, 10531472], [10531473, 11283720], [11283721, 12035968], [12035969, 12788216], [12788217, 13540464], [13540465, 14292712], [14292713, 15044979]] SRR13695457 file size 5091241 SRR13695457 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695457 SRR13695457_1.fastq SRR13695457_2.fastq Input file: SRR13695457_1.fastq Paired file: SRR13695457_2.fastq trimmed: SRR13695457-trimmed-pair1.fastq, SRR13695457-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 04:26:51 2025 >> started Wed Feb 12 04:27:06 2025 >> done (15.807s) 15044979 read pairs processed; of these: 81 ( 0.00%) short read pairs filtered out after trimming by size control 437 ( 0.00%) empty read pairs filtered out after trimming by size control 15044461 (100.00%) read pairs available; of these: 2106294 (14.00%) trimmed read pairs available after processing 12938167 (86.00%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 2 0.00% 20 1 0.00% 21 2 0.00% 22 0 0.00% 23 5 0.00% 24 2 0.00% 25 0 0.00% 26 1 0.00% 27 0 0.00% 28 1 0.00% 29 1 0.00% 30 3 0.00% 31 1 0.00% 32 5 0.00% 33 3 0.00% 34 6 0.00% 35 9 0.00% 36 3 0.00% 37 1 0.00% 38 6 0.00% 39 12 0.00% 40 6 0.00% 41 4 0.00% 42 16 0.00% 43 12 0.00% 44 11 0.00% 45 18 0.00% 46 18 0.00% 47 30 0.00% 48 32 0.00% 49 40 0.00% 50 41 0.00% 51 57 0.00% 52 54 0.00% 53 59 0.00% 54 73 0.00% 55 81 0.00% 56 117 0.00% 57 90 0.00% 58 150 0.00% 59 143 0.00% 60 215 0.00% 61 235 0.00% 62 248 0.00% 63 328 0.00% 64 373 0.00% 65 413 0.00% 66 466 0.00% 67 531 0.00% 68 612 0.00% 69 736 0.00% 70 839 0.01% 71 1004 0.01% 72 1165 0.01% 73 1234 0.01% 74 1548 0.01% 75 1761 0.01% 76 1878 0.01% 77 2188 0.01% 78 2299 0.02% 79 2728 0.02% 80 2946 0.02% 81 3495 0.02% 82 3860 0.03% 83 4442 0.03% 84 4887 0.03% 85 5463 0.04% 86 6044 0.04% 87 6487 0.04% 88 7211 0.05% 89 7458 0.05% 90 8069 0.05% 91 9039 0.06% 92 9447 0.06% 93 10315 0.07% 94 11197 0.07% 95 12140 0.08% 96 12815 0.09% 97 13588 0.09% 98 14279 0.09% 99 15084 0.10% 100 16012 0.11% 101 16420 0.11% 102 17568 0.12% 103 18582 0.12% 104 19486 0.13% 105 20076 0.13% 106 21525 0.14% 107 22478 0.15% 108 23214 0.15% 109 24013 0.16% 110 24979 0.17% 111 25388 0.17% 112 26496 0.18% 113 26996 0.18% 114 28326 0.19% 115 29188 0.19% 116 30451 0.20% 117 31310 0.21% 118 32925 0.22% 119 32778 0.22% 120 34628 0.23% 121 35203 0.23% 122 35811 0.24% 123 36658 0.24% 124 37144 0.25% 125 38606 0.26% 126 39303 0.26% 127 40166 0.27% 128 41137 0.27% 129 41268 0.27% 130 42587 0.28% 131 42961 0.29% 132 44224 0.29% 133 44047 0.29% 134 45015 0.30% 135 46155 0.31% 136 46073 0.31% 137 48129 0.32% 138 47985 0.32% 139 50093 0.33% 140 50619 0.34% 141 50964 0.34% 142 52082 0.35% 143 51566 0.34% 144 52347 0.35% 145 53594 0.36% 146 54396 0.36% 147 55044 0.37% 148 55988 0.37% 149 56518 0.38% 150 57588 0.38% 151 12938167 86.00% 15044461 reads passed initial QC criterion=sequence-density sequence-density=0.27 sequence-density-rank=1 fanout-score=1.97 fanout-score-rank=33 prefix-density=0.27 prefix-fanout=2.0 sequence=CGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTTCGAGCGAAGAAGGT criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=314.47 fanout-score-rank=1 prefix-density=0.25 prefix-fanout=16.8 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGAGTATGAATGTGTTCTCTGTG criterion=sequence-density sequence-density=0.42 sequence-density-rank=1 fanout-score=1.98 fanout-score-rank=35 prefix-density=0.42 prefix-fanout=2.0 sequence=AACCGCACCCCGGCACA criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=50.23 fanout-score-rank=1 prefix-density=0.07 prefix-fanout=4.5 sequence=TGGCCATGTAAAACACAATATTTTTCAGTATGAAAGCTTTAGTGATAGCTATTCTTATAGCTA SRR13695457 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 04:27:48 Started mapping on | Feb 12 04:27:49 Finished on | Feb 12 04:29:49 Mapping speed, Million of reads per hour | 451.33 Number of input reads | 15044461 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 14066550 Uniquely mapped reads % | 93.50% Average mapped length | 293.69 Number of splices: Total | 13843179 Number of splices: Annotated (sjdb) | 13518121 Number of splices: GT/AG | 13553037 Number of splices: GC/AG | 227691 Number of splices: AT/AC | 8279 Number of splices: Non-canonical | 54172 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.03% Deletion average length | 2.90 Insertion rate per base | 0.02% Insertion average length | 2.37 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 355406 % of reads mapped to multiple loci | 2.36% Number of reads mapped to too many loci | 55912 % of reads mapped to too many loci | 0.37% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.67% % of reads unmapped: other | 0.09% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 622669 622669 622669 N_multimapping 355406 355406 355406 N_noFeature 628761 13746734 817223 N_ambiguous 219515 1423 87160 UnstrandedReadsAssigned:13218274 PositiveStrandReadsAssigned:318393 NegativeStrandReadsAssigned:13162167 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695457 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695457-trimmed-pair1.fastq SRR13695457-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 15,044,461 reads, 13,171,489 reads pseudoaligned [quant] estimated average fragment length: 241.502 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,114 rounds 52401 SRR13695457.ke.tsv 34699 SRR13695457.se.tsv 87100 total ==> SRR13695457.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1777.5 655 26.2654 Potri.005G024800.1.v4.1 1035 794.498 360 32.297 Potri.004G059700.1.v4.1 961 720.572 0 0 Potri.007G009000.2.v4.1 1416 1175.5 0 0 Potri.003G141000.2.v4.1 2943 2702.5 805.525 21.2454 Potri.016G087400.1.v4.1 270 87.9761 698 565.513 Potri.015G069301.1.v4.1 564 331.227 0 0 Potri.010G195200.1.v4.1 1773 1532.5 75 3.4883 Potri.012G127500.1.v4.1 977 736.53 39 3.77421 ==> SRR13695457.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 68 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 188 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 14 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 14 SRR13695457 completed mapping pipeline successfully