Starting /dee2/code/volunteer_pipeline.sh SRR13695458 current disk space = 3049191104512 free memory = 1575097912 SRR13695458 SRAfilesize 77c948a0d91f17b78d0b226b171cca28 SRR13695458.sra SRR13695458.sra file validated SRR13695458 is paired end SRR13695458 is conventional basespace SRR13695458 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695458_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.6405 37.0 37.0 37.0 37.0 37.0 2 36.26 37.0 37.0 37.0 37.0 37.0 3 36.4955 37.0 37.0 37.0 37.0 37.0 4 36.6225 37.0 37.0 37.0 37.0 37.0 5 36.576 37.0 37.0 37.0 37.0 37.0 6 36.58 37.0 37.0 37.0 37.0 37.0 7 36.5365 37.0 37.0 37.0 37.0 37.0 8 36.6065 37.0 37.0 37.0 37.0 37.0 9 36.526 37.0 37.0 37.0 37.0 37.0 10-14 36.564 37.0 37.0 37.0 37.0 37.0 15-19 36.519 37.0 37.0 37.0 37.0 37.0 20-24 36.484100000000005 37.0 37.0 37.0 37.0 37.0 25-29 36.436699999999995 37.0 37.0 37.0 37.0 37.0 30-34 36.4961 37.0 37.0 37.0 37.0 37.0 35-39 36.3971 37.0 37.0 37.0 37.0 37.0 40-44 36.431 37.0 37.0 37.0 37.0 37.0 45-49 36.410399999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.3622 37.0 37.0 37.0 37.0 37.0 55-59 36.3505 37.0 37.0 37.0 37.0 37.0 60-64 36.328700000000005 37.0 37.0 37.0 37.0 37.0 65-69 36.2279 37.0 37.0 37.0 37.0 37.0 70-74 36.3582 37.0 37.0 37.0 37.0 37.0 75-79 36.2764 37.0 37.0 37.0 37.0 37.0 80-84 36.204299999999996 37.0 37.0 37.0 37.0 37.0 85-89 36.26200000000001 37.0 37.0 37.0 37.0 37.0 90-94 36.1745 37.0 37.0 37.0 37.0 37.0 95-99 36.1724 37.0 37.0 37.0 37.0 37.0 100-104 36.120200000000004 37.0 37.0 37.0 37.0 37.0 105-109 36.1746 37.0 37.0 37.0 37.0 37.0 110-114 36.0774 37.0 37.0 37.0 37.0 37.0 115-119 36.0783 37.0 37.0 37.0 37.0 37.0 120-124 35.98899999999999 37.0 37.0 37.0 37.0 37.0 125-129 35.9476 37.0 37.0 37.0 37.0 37.0 130-134 35.888099999999994 37.0 37.0 37.0 37.0 37.0 135-139 35.9807 37.0 37.0 37.0 37.0 37.0 140-144 35.8631 37.0 37.0 37.0 37.0 37.0 145-149 35.648399999999995 37.0 37.0 37.0 37.0 37.0 150-151 35.4505 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 0.0 22 0.0 23 3.0 24 1.0 25 3.0 26 4.0 27 6.0 28 14.0 29 16.0 30 33.0 31 31.0 32 71.0 33 68.0 34 114.0 35 316.0 36 2953.0 37 366.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.625 14.224999999999998 8.825 39.324999999999996 2 21.948769462581616 14.088397790055248 34.329482672024106 29.633350075339028 3 18.925 16.825000000000003 27.725 36.525 4 21.15 21.275 24.95 32.625 5 23.775 29.299999999999997 23.025000000000002 23.9 6 22.375 33.4 22.95 21.275 7 15.174999999999999 31.075000000000003 37.775 15.975 8 18.175 28.525 31.374999999999996 21.925 9 17.125 26.625 34.1 22.15 10-14 19.13 30.264999999999997 26.695 23.91 15-19 19.595000000000002 27.839999999999996 28.29 24.275 20-24 20.865000000000002 28.725 27.275 23.135 25-29 20.34 28.42 27.01 24.23 30-34 19.900000000000002 28.249999999999996 28.48 23.369999999999997 35-39 20.745 28.38 27.51 23.365 40-44 21.04 28.499999999999996 27.279999999999998 23.18 45-49 19.865 28.685 26.555 24.895 50-54 20.285 28.99 27.485 23.24 55-59 20.4 27.725 27.245 24.63 60-64 19.869999999999997 28.89 27.565 23.674999999999997 65-69 20.935000000000002 27.76 27.095000000000002 24.21 70-74 20.25 27.91 27.345000000000002 24.495 75-79 20.825 27.97 27.634999999999998 23.57 80-84 20.544999999999998 27.79 28.055000000000003 23.61 85-89 21.34 28.43 26.33 23.9 90-94 20.87 27.700000000000003 27.255000000000003 24.175 95-99 20.995 27.155 27.705000000000002 24.145 100-104 20.895 27.47 27.925 23.71 105-109 21.055 27.505000000000003 26.775 24.665 110-114 21.26 27.46 26.26 25.019999999999996 115-119 21.55 27.755000000000003 27.005000000000003 23.69 120-124 20.86 28.53 26.32 24.29 125-129 21.765 28.660000000000004 25.919999999999998 23.655 130-134 21.3 28.265 26.284999999999997 24.15 135-139 22.445 27.435 26.615 23.505000000000003 140-144 22.305 27.275 25.955000000000002 24.465 145-149 21.560000000000002 27.62 25.590000000000003 25.230000000000004 150-151 21.075 27.487499999999997 26.5125 24.925 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.0 19 0.0 20 3.5 21 3.5 22 1.0 23 1.5 24 3.0 25 4.5 26 2.5 27 5.0 28 5.0 29 4.5 30 8.0 31 13.5 32 21.5 33 32.5 34 46.5 35 65.0 36 91.5 37 114.0 38 132.5 39 173.0 40 208.0 41 203.5 42 214.5 43 236.5 44 255.5 45 259.0 46 243.5 47 230.0 48 191.0 49 198.0 50 210.0 51 169.5 52 133.5 53 110.0 54 92.0 55 82.0 56 63.5 57 41.5 58 38.5 59 29.5 60 17.0 61 9.5 62 11.0 63 8.0 64 1.0 65 2.5 66 2.5 67 0.5 68 2.0 69 1.5 70 0.5 71 0.5 72 0.0 73 0.0 74 0.5 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.44999999999999996 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.10000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 68.34341906202724 45.175 2 20.04538577912254 26.5 3 6.9969742813918305 13.875000000000002 4 2.6096822995461424 6.9 5 1.2102874432677762 4.0 6 0.49167927382753407 1.95 7 0.11346444780635401 0.525 8 0.07564296520423601 0.4 9 0.11346444780635401 0.675 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTCGAGTACAAATAATATAAGGCGTTGTTGAAATGCTCATCAGATCACAT 9 0.22499999999999998 No Hit CAGTAGCAAACCAGGTGAAAAGGAAACTGAATGGCTCTGGGAGCTCCAAA 9 0.22499999999999998 No Hit CACGTAGTAGGCCATGGCAAACTGAATCTGGTTCTCAGGTGGATAAATAG 9 0.22499999999999998 No Hit GTTTCTTGAGCAGCTCCTTTAATTCCAAGAAGCTGGTTAAAGCTGGAACC 8 0.2 No Hit CACTGGCGCTGTCTTCTTCTCTAAAGCTTTAGTGCCCAGTTATGGGCACT 8 0.2 No Hit CCTGTTGTAAATAACCTCAGCAATCTCAACAGTTCCAGCTTGTTCAAACA 7 0.17500000000000002 No Hit GCGACATGGAAAAATAAGGACATGCCCGGTGAGGGAGGGAGAGAACATAG 7 0.17500000000000002 No Hit CCTGGGTTATGTTATATTTTTAAAAGAAAAATAACTGAATTAATACAAGA 7 0.17500000000000002 No Hit AGTTGAATGGCCAGGAAAGGTATTTTTCCCAGCTTCAATAGCTGGTCCAT 6 0.15 No Hit GTTCACAATTTCACTTCTATCCTGCCCCGGAAACTTCCTGACAACGAATA 6 0.15 No Hit CTTGTAAAGGACCGTTCACCGCCTGTTTATTGGTGAGATCCCTCAAGAAA 6 0.15 No Hit AATGGCACTAGGTCGAGCTTCCCTCAACTTGAAGACGCTTTCAATTACAG 6 0.15 No Hit ATCCTTTTCCACGTTTTTCCAGTCCAGCTTTAAATTTCGCAGGACCTTAA 6 0.15 No Hit CCCCACTAGAAACCCAAAAGAAAAGGATTGTGACTTGATGCTTGAAGCAG 6 0.15 No Hit CTCAACAACACATCACCACTGGAATTCAAATGATGATGTGTAGGCTTGTC 6 0.15 No Hit CTTACAATAATGCTTACAGTTCCAGCCAGATTCTTGATACCTGGACAGGT 6 0.15 No Hit CTCCAGTCTCAGGCTTGCTCTTGGTCACACTTAGGGTGATTTTACCTGTT 6 0.15 No Hit TTCATGAGCTCGTTACATCACAAGTTAACATTACAAGCCAATAGTCCCAG 6 0.15 No Hit GTCCTTAGTCTCTTCATCTGAATCCTCGTCAGATGCAGAATCCAGAGCTT 6 0.15 No Hit CTGCACTTGACGCGTGTTGTCGAATCCGATTATACGGATAAAGGCGTTAG 6 0.15 No Hit GGAGAGAATAGATACATACCAGTTTGCTTTTCCAAGCAACAATTAGACTG 6 0.15 No Hit CAACTATCTCTCCAGCCTCAGCAGCAGCAAGCACAAGCTCTGGCTGTAGA 5 0.125 No Hit ACCATGCTTGATTCTTGGATAGAAAAGGCCACTGGTCTTGAAAACCCTTT 5 0.125 No Hit GTTGGTTGTACCCACCTTGCTCTTGGTGGTTGTACCCACCTTGTGTCTCC 5 0.125 No Hit ACATGCTTTGAGTCTGTCAATAGAGCCATCAAAATGATATTTGGTTCGGA 5 0.125 No Hit CGCCAGATATTGATTCTCTCGTCATCTGTGAGCAGTGACCAGCGTTTCAA 5 0.125 No Hit AGAGGTATAGCAGCACATCAGATAGCTCTTCTGCTAGATGCTCCTTGTCA 5 0.125 No Hit CCGCTGTTGAGGTGATATTGGTTCCCACCGCTCCTGTTGAATGGCACGCT 5 0.125 No Hit GGTTGCTTCAACCTCGGATTGCCCACCAGACAAAAACATGATTCCAGGGA 5 0.125 No Hit GTCTGGTACTGGTAGCCATTTCCAAAGTAGTTGCCGTGACTGGGAGAGAA 5 0.125 No Hit CTGCAGAAATGAAAATGGTGAAACTTTTGTTACATGGAGTAGACTTCTTG 5 0.125 No Hit TCTCTGTGTCGCGACCAACACGAATAGAAGCTCCACGATCAGCCACACCC 5 0.125 No Hit GAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCA 5 0.125 No Hit CGAAGAACCAAGTCCCTAGGTTATGCATATTGTCTTTTTTGAAGGGTGGG 5 0.125 No Hit CTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGTTTAATGACCG 5 0.125 No Hit CACCATCATCACGGGTTGTTCCTGTAGAATTAATATAGATATAAATTGGT 5 0.125 No Hit ATCACATATTTCAATTTGATTTACAACAATTTCTTAGTTACGTTTCTTAA 5 0.125 No Hit GTCCAAAATCTCTTCAGTATCCCCATAACCCTGTTCTGTAAGGAGAACTG 5 0.125 No Hit CGTCCTCATTTGCAGGAGCATGGATCACAGGTGCAGTTTGATCCACACTT 5 0.125 No Hit ATCACATTACATCGCTACTTCCATTGATCGGCAACAATGTGAGCCAAATC 5 0.125 No Hit CATCCAATATACATATACACATGAACATGATGATTGAACGATTCAGTCAT 5 0.125 No Hit GCTGTAGTAAGGAAGTTGCTGGGCGAAAGTATTCAGGAAGCAGGGGACTT 5 0.125 No Hit ATCAAGATACAATCCAGTACACAATATCTGAAAATTCTCTCCGCCACAAA 5 0.125 No Hit CTCTCACTTTCCGGGGGCGAAGTTTGTGGCATATGCCCAGGCGTTGTTGT 5 0.125 No Hit GAGCTATGATAGCCAGGCCAGTTGACCCTGCCGGAAGTAGAGGAGCCGGG 5 0.125 No Hit GCCCACAATCACGCTTGTAAGGAAGACATGGTGTTGATGTTGGTGGTTGG 5 0.125 No Hit GTGCAGAGGAAGAATGATGATTCAACTTTCCGCTTCCATGCTAGGCTCCT 5 0.125 No Hit TCGTTTTCGTGCGTCGCGTGCGTGGTGCGCTCGCAAAGTGAGGCTCAACG 5 0.125 No Hit ATACATAGGGATTCACAAGGGGGGAGAATTTTTTGTTTCTGGACAGTGAA 5 0.125 No Hit CATCCCTTCTCTCCACCTGGAAAACCCCCTGTTAATCCCATCAGCTCTTT 5 0.125 No Hit CAGCCCAGAACCTCCAAAGCTCCCTTTCTGCTGTGCCTTGGCTCTGAGCT 5 0.125 No Hit AGCTCACGAAGACCACAGTGAATTGGGGTCACAGAAGTGGATCTTGAAGG 5 0.125 No Hit CAGGGCTCCATTTCCCTTTCGCCTTGTAGGCTGAACAGCTGCCAGGGATT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.15 0.0 0.0 0.0 0.0 70-71 0.175 0.0 0.0 0.0 0.0 72-73 0.2 0.0 0.0 0.0 0.0 74-75 0.25 0.0 0.0 0.0 0.0 76-77 0.275 0.0 0.0 0.0 0.0 78-79 0.4 0.0 0.0 0.0 0.0 80-81 0.5375 0.0 0.0 0.0 0.0 82-83 0.5874999999999999 0.0 0.0 0.0 0.0 84-85 0.6625 0.0 0.0 0.0 0.0 86-87 0.7749999999999999 0.0 0.0 0.0 0.0 88-89 1.1625 0.0 0.0 0.0 0.0 90-91 1.35 0.0 0.0 0.0 0.0 92-93 1.5125000000000002 0.0 0.0 0.0 0.0 94-95 1.575 0.0 0.0 0.0 0.0 96-97 1.7 0.0 0.0 0.0 0.0 98-99 1.8125 0.0 0.0 0.0 0.0 100-101 2.05 0.0 0.0 0.0 0.0 102-103 2.25 0.0 0.0 0.0 0.0 104-105 2.45 0.0 0.0 0.0 0.0 106-107 3.0125 0.0 0.0 0.0 0.0 108-109 3.4875 0.0 0.0 0.0 0.0 110-111 4.1375 0.0 0.0 0.0 0.0 112-113 4.7 0.0 0.0 0.0 0.0 114-115 5.125 0.0 0.0 0.0 0.0 116-117 5.625 0.0 0.0 0.0 0.0 118-119 6.074999999999999 0.0 0.0 0.0 0.0 120-121 6.512499999999999 0.0 0.0 0.0 0.0 122-123 7.2125 0.0 0.0 0.0 0.0 124-125 8.075 0.0 0.0 0.0 0.0 126-127 9.025 0.0 0.0 0.0 0.0 128-129 9.649999999999999 0.0 0.0 0.0 0.0 130-131 10.4625 0.0 0.0 0.0 0.0 132-133 11.3875 0.0 0.0 0.0 0.0 134-135 12.5 0.0 0.0 0.0 0.0 136-137 13.5125 0.0 0.0 0.0 0.0 138-139 14.45 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTGTGG 10 0.006830828 145.0 3 GCACCAG 10 0.006830828 145.0 1 >>END_MODULE SRR13695458 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695458_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.24675 37.0 37.0 37.0 37.0 37.0 2 36.1915 37.0 37.0 37.0 37.0 37.0 3 36.2015 37.0 37.0 37.0 37.0 37.0 4 36.1355 37.0 37.0 37.0 37.0 37.0 5 36.304 37.0 37.0 37.0 37.0 37.0 6 36.2825 37.0 37.0 37.0 37.0 37.0 7 36.203 37.0 37.0 37.0 37.0 37.0 8 36.206 37.0 37.0 37.0 37.0 37.0 9 36.2225 37.0 37.0 37.0 37.0 37.0 10-14 36.259 37.0 37.0 37.0 37.0 37.0 15-19 36.186400000000006 37.0 37.0 37.0 37.0 37.0 20-24 36.1426 37.0 37.0 37.0 37.0 37.0 25-29 36.13975000000001 37.0 37.0 37.0 37.0 37.0 30-34 36.04465 37.0 37.0 37.0 37.0 37.0 35-39 36.054950000000005 37.0 37.0 37.0 37.0 37.0 40-44 36.06765 37.0 37.0 37.0 37.0 37.0 45-49 36.01915 37.0 37.0 37.0 37.0 37.0 50-54 36.02055 37.0 37.0 37.0 37.0 37.0 55-59 35.952149999999996 37.0 37.0 37.0 37.0 37.0 60-64 35.91365 37.0 37.0 37.0 37.0 37.0 65-69 35.9485 37.0 37.0 37.0 37.0 37.0 70-74 35.86435 37.0 37.0 37.0 37.0 37.0 75-79 35.91055 37.0 37.0 37.0 37.0 37.0 80-84 35.87505 37.0 37.0 37.0 37.0 37.0 85-89 35.79485 37.0 37.0 37.0 37.0 37.0 90-94 35.850649999999995 37.0 37.0 37.0 37.0 37.0 95-99 35.81765 37.0 37.0 37.0 37.0 37.0 100-104 35.73235 37.0 37.0 37.0 37.0 37.0 105-109 35.728750000000005 37.0 37.0 37.0 37.0 37.0 110-114 35.61585 37.0 37.0 37.0 37.0 37.0 115-119 35.58785 37.0 37.0 37.0 37.0 37.0 120-124 35.599450000000004 37.0 37.0 37.0 37.0 37.0 125-129 35.50574999999999 37.0 37.0 37.0 37.0 37.0 130-134 35.37075 37.0 37.0 37.0 34.6 37.0 135-139 35.2886 37.0 37.0 37.0 34.6 37.0 140-144 35.17294999999999 37.0 37.0 37.0 34.6 37.0 145-149 34.91435 37.0 37.0 37.0 25.0 37.0 150-151 34.546875 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 2.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 2.0 13 11.0 14 2.0 15 3.0 16 7.0 17 1.0 18 0.0 19 3.0 20 3.0 21 4.0 22 4.0 23 5.0 24 2.0 25 8.0 26 11.0 27 10.0 28 9.0 29 15.0 30 22.0 31 28.0 32 62.0 33 111.0 34 189.0 35 537.0 36 2713.0 37 235.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.444165621079044 22.082810539523212 13.14930991217064 26.3237139272271 2 29.925 25.275 28.95 15.85 3 23.45 27.425 29.799999999999997 19.325 4 23.95 30.675 25.324999999999996 20.05 5 26.700000000000003 34.925 22.125 16.25 6 23.549999999999997 37.55 20.375 18.525 7 20.65 23.575 36.325 19.45 8 24.4 26.200000000000003 27.85 21.55 9 25.6 23.599999999999998 26.650000000000002 24.15 10-14 25.03 29.315 25.355 20.3 15-19 24.245 28.425 26.115 21.215 20-24 24.712298609026316 27.849494646252378 26.043230261182828 21.39497648353848 25-29 24.06805103827871 27.690768076057044 27.32549412059044 20.915686765073804 30-34 23.987990993244935 27.390542907180386 27.045283962972228 21.57618213660245 35-39 24.578433825369025 28.241180885664246 27.06529897423067 20.115086314736054 40-44 24.013009757317988 27.990993244933698 26.509882411808857 21.486114585939458 45-49 23.567675756817614 27.730798098573928 27.280460345258945 21.421065799349513 50-54 24.193144858643983 28.941706279709784 26.37978483862897 20.485364023017265 55-59 23.6977733299975 27.595696772579437 27.335501626219667 21.3710282712034 60-64 23.29246935201401 27.73580185138854 26.91018263697773 22.061546159619716 65-69 24.33216608304152 27.788894447223612 26.348174087043525 21.530765382691346 70-74 23.837878408806603 27.92594445834376 27.010257693269953 21.225919439579684 75-79 23.687765824368277 27.890918188641482 26.549912434325744 21.871403552664496 80-84 24.213159869902427 28.236177132849637 26.594946209657245 20.955716787590692 85-89 24.588441330998247 27.790843132349263 26.740055041280964 20.88066049537153 90-94 24.68851638729047 27.31548661496122 27.190392794595947 20.805604203152363 95-99 24.043032274205654 28.081060795596695 26.474856142106578 21.40105078809107 100-104 24.59844883662747 27.220415311483613 28.246184638478862 19.934951213410056 105-109 24.113084813610207 28.241180885664246 26.830122591943955 20.815611708781585 110-114 24.47835876907681 27.91593695271454 26.695021265949464 20.91068301225919 115-119 24.718538904178132 28.376282211658744 27.045283962972228 19.859894921190893 120-124 25.789342006504878 27.78083562672004 25.81436077057793 20.61546159619715 125-129 26.2546910182637 27.52064048036027 26.139604703527645 20.085063797848388 130-134 26.0471400690587 28.168943602061752 26.642646249311912 19.141270079567633 135-139 26.396116893514808 27.331865492393913 26.36108887109688 19.910928742994397 140-144 27.695771828871653 27.530647985989493 25.94946209657243 18.824118088566426 145-149 28.42131598699024 27.09532149111834 25.298974230673004 19.18438829121841 150-151 28.650068810208936 28.737645439759792 24.771675215813836 17.84061053421744 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.0 4 0.5 5 0.5 6 0.0 7 0.5 8 1.5 9 1.0 10 0.0 11 1.5 12 2.5 13 2.0 14 1.0 15 0.5 16 0.5 17 0.5 18 0.5 19 1.0 20 1.0 21 0.5 22 1.0 23 1.0 24 1.5 25 2.5 26 1.5 27 0.5 28 3.0 29 9.0 30 10.5 31 6.5 32 8.0 33 13.5 34 22.5 35 31.0 36 38.0 37 80.0 38 128.5 39 142.0 40 153.5 41 192.5 42 227.0 43 249.0 44 296.0 45 304.5 46 302.0 47 298.0 48 237.5 49 216.5 50 199.5 51 151.0 52 130.5 53 115.5 54 99.5 55 76.0 56 66.0 57 53.0 58 29.0 59 19.5 60 14.5 61 10.0 62 6.5 63 6.0 64 4.0 65 3.5 66 2.5 67 2.5 68 2.5 69 1.5 70 0.5 71 0.0 72 0.5 73 0.5 74 0.5 75 0.5 76 0.0 77 0.0 78 1.0 79 2.0 80 1.0 81 0.0 82 0.0 83 1.0 84 1.0 85 0.0 86 0.0 87 0.0 88 1.0 89 1.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.375 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.06999999999999999 25-29 0.075 30-34 0.075 35-39 0.075 40-44 0.075 45-49 0.075 50-54 0.075 55-59 0.075 60-64 0.075 65-69 0.05 70-74 0.075 75-79 0.075 80-84 0.075 85-89 0.075 90-94 0.075 95-99 0.075 100-104 0.075 105-109 0.075 110-114 0.075 115-119 0.075 120-124 0.075 125-129 0.075 130-134 0.08499999999999999 135-139 0.08 140-144 0.075 145-149 0.075 150-151 0.08750000000000001 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.30000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 69.9108469539376 47.05 2 19.01931649331352 25.6 3 6.8350668647845465 13.8 4 2.265973254086181 6.1 5 1.2258543833580982 4.125 6 0.44576523031203563 1.7999999999999998 7 0.18573551263001484 0.8750000000000001 8 0.03714710252600297 0.2 9 0.07429420505200594 0.44999999999999996 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTCTTGTCGTAGCAATCTTGCCGGATGTGCCCGAGGAACACCGTGACATT 9 0.22499999999999998 No Hit ATGATGAGCTGAAAATGAAATTAAAGTCTGAAGGCAGCGAAATAGTTGTC 9 0.22499999999999998 No Hit TGAGTTTCAACTGTCCAATGACGATTACTTCTCCAAGAATAATGAGTTCG 8 0.2 No Hit CGGTGAGGTAACTGACCCAATCTACCCAGGTGGAAGCTTCGACCCACTGG 7 0.17500000000000002 No Hit GAGGTGAAGGAGAGAGTGGTGACGAGGAGGGATTTGTAGAGCCACCAGAG 7 0.17500000000000002 No Hit GTCAGCAGTAGCACAAACAAACTTTCCCGAGGCATCTGTAGAGCATTGCG 7 0.17500000000000002 No Hit AAATCATTGACTTCCAAACAAGTCCTCTGTCTGTAGATGGAAGAGATTTT 7 0.17500000000000002 No Hit GCACACTGCTGGCCACACTGCTAGATCACTATGTTCTCTCCCTCCCTCAC 7 0.17500000000000002 No Hit ACACACCAGGAACCGAGCCATTTGAATTGTGGGAAGGACTCGATCGTAGA 6 0.15 No Hit TGCCAGAGTCTTATACTTTGTCCATGAATATGGTGCTAAAAAAAACCCTG 6 0.15 No Hit TGAAACTTTACGTCCAGTGGACATGCATAGTGGGATGGAAGTTCGCCTTG 6 0.15 No Hit CCAGGGCAGCCTGGTGATAGGTTCAGGCTAAAGGAAGAAGAGAAGGGCAA 6 0.15 No Hit ATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAAT 6 0.15 No Hit GGCTTTCTGAGTTTTTCTTGGGGGAACAAGTACAAATTGACCAAACAAGT 6 0.15 No Hit CTTCAGGTCCCCTTAGTTGCAGGTACTGTAAACCGTGGTAGCGAAGTGAT 6 0.15 No Hit TCTTCTGGCACCGTTCCTTCTGAATATTTTGCTTACAAGTTCCTGGCTCA 6 0.15 No Hit CAGAGAACACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 6 0.15 No Hit ACTATATGGAGAGGATGTCCCAGCTGGAGCTGCTAGGTTTAGCGATCTTG 6 0.15 No Hit AGAAGTTGCTCCAGAGGAGGTTGCTGTGCCTGCCAAGCAGCTCCAAGCTT 6 0.15 No Hit GTTGAAGCAACTGGAGCGACAGTTAAAGACAGGGGTTGAGCGTATCCGCT 6 0.15 No Hit CTCCAAGAAACCATTTTCCAATTTCCTGTCCTCTACAAAATTCCAAGGAT 5 0.125 No Hit AGTGACTATTCATGCAAGTTTTGGGGAGCGATACTTGTCATCTGTTCTCT 5 0.125 No Hit TGTTGATGTAAAGAATATTCTTGTTGAGATGGGAACTTACTTCCAAGTAC 5 0.125 No Hit TCAAAGAAGCAAAAATGGCAGCCACTACCATGATGACTGCTACTGCTCTC 5 0.125 No Hit TACCCTAGCTGGCATTCTTGCCGGAGGTGCACTTCTTGTTTACAGCACAA 5 0.125 No Hit CTTTCTCAAGATTGAATCTATATCTGGAACATTGGTCATGCGATGGGTCA 5 0.125 No Hit GTGTGCAACAAGAACTAAAGAGAAACATTGAAGAACAAGCATCATCAACT 5 0.125 No Hit CTGGCATGATTGTTACAGGCATGGAAGTTGCAGAAATTGATGGAGCACCA 5 0.125 No Hit CTGGACTATGTGGAAGCTACCCATGTTTGGATGCACTGAGGCATCTCAAG 5 0.125 No Hit CTCCGTCGACCATGAAAGCTCTCATGTAAAGCTCTACTGGTGTGGGTTTA 5 0.125 No Hit GTTAGTCACATATATTTCCAAGATGAAGGCCTTTCTTATCGCATGCATTC 5 0.125 No Hit GCATGGAAGAAATAGAAGAGATCTCTATTGCAGAAGAGAAAGTAGTGGAC 5 0.125 No Hit GATTCTTTATGTGCTTCTTGCAGGATTCTTGCCATTTCAAGATGAGAATG 5 0.125 No Hit CCCAACAAGTTCATCACCACAATGTCTTGCTCAAATCTGACAATGTTGGT 5 0.125 No Hit ACCGTGCTACTGGGCAAACCCCAAAGAAGCTCCTTGGTTGCTTCTGAAGT 5 0.125 No Hit GTGATTCGCGTGCGTGCTGGCTCGGATCAATTCCTCTTTGAGTGTACGGG 5 0.125 No Hit AGCTTGAGCAAATTCAGTTTCTAAGCAAAAGCTTTCCAGGCCCCTTTATC 5 0.125 No Hit CAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGC 5 0.125 No Hit GGGTATCCTATCAGTCTGTGATGAGCCACTTGTTTCAGTTGACTTCAGGT 5 0.125 No Hit CTGAAATCATACTTGAAGGAGGCAAGAAAAGTTTCTTCATCAGTCAAGCC 5 0.125 No Hit GTCTGTATGCAGGCATTAACATTAGCGGCATCAATGGAGAAGTGATGCCA 5 0.125 No Hit ATCTACCCAGGTGGAAGCTTCGACCCACTGGGCTTGGCTGATGACCCAGA 5 0.125 No Hit TATTTTTGGAAAGTACAGACTGTTGAGGAGCTCGTCTTGAGGGATTTCAA 5 0.125 No Hit AGAAAGATGAGCGCAAGGGTGAGACACAAGGTGGGTACAACCAACAGGAT 5 0.125 No Hit CTCCACTGAACCCAAAGGGCTCAAATCTGCTACTAAGAATCCAACCTGGA 5 0.125 No Hit CAGAAATCTTGCTTGATGGTGAGCATGGCATTGACAGGACTTTCGAAGTA 5 0.125 No Hit CCCTTTGAAGCGCCTCAAGGATAGCTTTTGCTATCCTAACCTCATCAAAT 5 0.125 No Hit TGGAAGAATTGTTTACATTGGCATGCCTCTAGTGGCTGCTGTCACGGAGC 5 0.125 No Hit TGAAAAGTTCTCAAGTGTCAGCGTTTAAGTTTTTAGTCTGCATTGAAATC 5 0.125 No Hit TACAGATTGTGCCTCTGTTCTTGAGGGATTTTGTATATGACAATGTTGCA 5 0.125 No Hit GGACAGAAGAATTTTGTGACTGCGCAAGGTCGCACTAGCCCTGATCAAAA 5 0.125 No Hit GGAAGCGAAGCTTGATACAGGGATAATATCCTGTGGATAGTCATAACAGT 5 0.125 No Hit CTTCAATCATGTCTTCCAAGCTAGGTGGAAAAGCTAAGCCTTTGAAGGCG 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.15 0.0 0.0 0.0 0.0 70-71 0.175 0.0 0.0 0.0 0.0 72-73 0.2 0.0 0.0 0.0 0.0 74-75 0.25 0.0 0.0 0.0 0.0 76-77 0.275 0.0 0.0 0.0 0.0 78-79 0.38749999999999996 0.0 0.0 0.0 0.0 80-81 0.5125 0.0 0.0 0.0 0.0 82-83 0.5625 0.0 0.0 0.0 0.0 84-85 0.6375 0.0 0.0 0.0 0.0 86-87 0.75 0.0 0.0 0.0 0.0 88-89 1.1375 0.0 0.0 0.0 0.0 90-91 1.325 0.0 0.0 0.0 0.0 92-93 1.5125000000000002 0.0 0.0 0.0 0.0 94-95 1.575 0.0 0.0 0.0 0.0 96-97 1.7 0.0 0.0 0.0 0.0 98-99 1.8125 0.0 0.0 0.0 0.0 100-101 2.025 0.0 0.0 0.0 0.0 102-103 2.2249999999999996 0.0 0.0 0.0 0.0 104-105 2.425 0.0 0.0 0.0 0.0 106-107 2.9875 0.0 0.0 0.0 0.0 108-109 3.4749999999999996 0.0 0.0 0.0 0.0 110-111 4.1375 0.0 0.0 0.0 0.0 112-113 4.7 0.0 0.0 0.0 0.0 114-115 5.125 0.0 0.0 0.0 0.0 116-117 5.625 0.0 0.0 0.0 0.0 118-119 6.074999999999999 0.0 0.0 0.0 0.0 120-121 6.512499999999999 0.0 0.0 0.0 0.0 122-123 7.2 0.0 0.0 0.0 0.0 124-125 8.05 0.0 0.0 0.0 0.0 126-127 9.025 0.0 0.0 0.0 0.0 128-129 9.649999999999999 0.0 0.0 0.0 0.0 130-131 10.4625 0.0 0.0 0.0 0.0 132-133 11.3875 0.0 0.0 0.0 0.0 134-135 12.5 0.0 0.0 0.0 0.0 136-137 13.5125 0.0 0.0 0.0 0.0 138-139 14.475 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGTCAA 10 0.006830828 145.0 1 GAGAACA 10 0.006830828 145.0 3 ACATTCA 10 0.006830828 145.0 9 AGAACAC 10 0.006830828 145.0 4 GAACACA 10 0.006830828 145.0 5 AGCATCA 10 0.006830828 145.0 4 CGTCGAG 10 0.006830828 145.0 145 CGAGAAA 10 0.006830828 145.0 9 CAGAGAA 25 8.7132835E-4 87.0 1 GGGGGGG 65 0.0076375785 13.384615 140-144 >>END_MODULE Read 811664 spots for SRR13695458.sra Written 811664 spots for SRR13695458.sra Read 811664 spots for SRR13695458.sra Written 811664 spots for SRR13695458.sra Read 811664 spots for SRR13695458.sra Written 811664 spots for SRR13695458.sra Read 811664 spots for SRR13695458.sra Written 811664 spots for SRR13695458.sra Read 811664 spots for SRR13695458.sra Written 811664 spots for SRR13695458.sra Read 811664 spots for SRR13695458.sra Written 811664 spots for SRR13695458.sra Read 811664 spots for SRR13695458.sra Written 811664 spots for SRR13695458.sra Read 811664 spots for SRR13695458.sra Written 811664 spots for SRR13695458.sra Read 811664 spots for SRR13695458.sra Written 811664 spots for SRR13695458.sra Read 811664 spots for SRR13695458.sra Written 811664 spots for SRR13695458.sra Read 811664 spots for SRR13695458.sra Written 811664 spots for SRR13695458.sra Read 811664 spots for SRR13695458.sra Written 811664 spots for SRR13695458.sra Read 811664 spots for SRR13695458.sra Written 811664 spots for SRR13695458.sra Read 811664 spots for SRR13695458.sra Written 811664 spots for SRR13695458.sra Read 811664 spots for SRR13695458.sra Written 811664 spots for SRR13695458.sra Read 811664 spots for SRR13695458.sra Written 811664 spots for SRR13695458.sra Read 811664 spots for SRR13695458.sra Written 811664 spots for SRR13695458.sra Read 811664 spots for SRR13695458.sra Written 811664 spots for SRR13695458.sra Read 811664 spots for SRR13695458.sra Written 811664 spots for SRR13695458.sra Read 811671 spots for SRR13695458.sra Written 811671 spots for SRR13695458.sra SRR ids: ['SRR13695458.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_im227d90 SRR13695458.sra spots: 16233287 blocks: [[1, 811664], [811665, 1623328], [1623329, 2434992], [2434993, 3246656], [3246657, 4058320], [4058321, 4869984], [4869985, 5681648], [5681649, 6493312], [6493313, 7304976], [7304977, 8116640], [8116641, 8928304], [8928305, 9739968], [9739969, 10551632], [10551633, 11363296], [11363297, 12174960], [12174961, 12986624], [12986625, 13798288], [13798289, 14609952], [14609953, 15421616], [15421617, 16233287]] SRR13695458 file size 5495080 SRR13695458 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695458 SRR13695458_1.fastq SRR13695458_2.fastq Input file: SRR13695458_1.fastq Paired file: SRR13695458_2.fastq trimmed: SRR13695458-trimmed-pair1.fastq, SRR13695458-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 04:30:30 2025 >> started Wed Feb 12 04:30:47 2025 >> done (16.992s) 16233287 read pairs processed; of these: 134 ( 0.00%) short read pairs filtered out after trimming by size control 11918 ( 0.07%) empty read pairs filtered out after trimming by size control 16221235 (99.93%) read pairs available; of these: 2980065 (18.37%) trimmed read pairs available after processing 13241170 (81.63%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 3 0.00% 20 0 0.00% 21 1 0.00% 22 2 0.00% 23 4 0.00% 24 0 0.00% 25 1 0.00% 26 4 0.00% 27 2 0.00% 28 3 0.00% 29 2 0.00% 30 7 0.00% 31 3 0.00% 32 9 0.00% 33 3 0.00% 34 3 0.00% 35 9 0.00% 36 9 0.00% 37 10 0.00% 38 19 0.00% 39 10 0.00% 40 22 0.00% 41 25 0.00% 42 25 0.00% 43 41 0.00% 44 37 0.00% 45 36 0.00% 46 52 0.00% 47 59 0.00% 48 87 0.00% 49 94 0.00% 50 89 0.00% 51 140 0.00% 52 164 0.00% 53 136 0.00% 54 206 0.00% 55 181 0.00% 56 253 0.00% 57 280 0.00% 58 312 0.00% 59 400 0.00% 60 466 0.00% 61 517 0.00% 62 597 0.00% 63 740 0.00% 64 845 0.01% 65 898 0.01% 66 1007 0.01% 67 1107 0.01% 68 1286 0.01% 69 1476 0.01% 70 1757 0.01% 71 1905 0.01% 72 2422 0.01% 73 2666 0.02% 74 3069 0.02% 75 3548 0.02% 76 3809 0.02% 77 4184 0.03% 78 4388 0.03% 79 5093 0.03% 80 5564 0.03% 81 6202 0.04% 82 7092 0.04% 83 7741 0.05% 84 8921 0.05% 85 9796 0.06% 86 10872 0.07% 87 11229 0.07% 88 12375 0.08% 89 12973 0.08% 90 14181 0.09% 91 14798 0.09% 92 15925 0.10% 93 17433 0.11% 94 18452 0.11% 95 19916 0.12% 96 21233 0.13% 97 22004 0.14% 98 23253 0.14% 99 24166 0.15% 100 25648 0.16% 101 25637 0.16% 102 26636 0.16% 103 27980 0.17% 104 29261 0.18% 105 31282 0.19% 106 32344 0.20% 107 33814 0.21% 108 34958 0.22% 109 36086 0.22% 110 36729 0.23% 111 36943 0.23% 112 38686 0.24% 113 39370 0.24% 114 40979 0.25% 115 42253 0.26% 116 43739 0.27% 117 45777 0.28% 118 46874 0.29% 119 47750 0.29% 120 48467 0.30% 121 49124 0.30% 122 49772 0.31% 123 51249 0.32% 124 51680 0.32% 125 53228 0.33% 126 54825 0.34% 127 56655 0.35% 128 57244 0.35% 129 58442 0.36% 130 59279 0.37% 131 59320 0.37% 132 59814 0.37% 133 61032 0.38% 134 60728 0.37% 135 62126 0.38% 136 63561 0.39% 137 64541 0.40% 138 66018 0.41% 139 67494 0.42% 140 66573 0.41% 141 67990 0.42% 142 68508 0.42% 143 68616 0.42% 144 69532 0.43% 145 69735 0.43% 146 70303 0.43% 147 71656 0.44% 148 73520 0.45% 149 73234 0.45% 150 74402 0.46% 151 13241170 81.63% 16221235 reads passed initial QC criterion=sequence-density sequence-density=0.81 sequence-density-rank=1 fanout-score=2.14 fanout-score-rank=16 prefix-density=0.85 prefix-fanout=2.0 sequence=TTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGG criterion=fanout-score sequence-density=0.05 sequence-density-rank=24 fanout-score=15.77 fanout-score-rank=1 prefix-density=0.18 prefix-fanout=4.2 sequence=AATGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTG criterion=sequence-density sequence-density=0.84 sequence-density-rank=1 fanout-score=2.32 fanout-score-rank=12 prefix-density=0.87 prefix-fanout=2.2 sequence=CCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAATCATCATGTCTAGCACCTGCGACACCTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAA criterion=fanout-score sequence-density=0.01 sequence-density-rank=24 fanout-score=14.54 fanout-score-rank=1 prefix-density=0.10 prefix-fanout=1.5 sequence=CCACCACTAAACCCAAAGCTCAAAAAAAATCTTAACTGCACCCGCTCATCCAGCAATGGCAGCAGCAACAATGGCCCTCTCCTCCCCTTCGCTAGCCGGAAAGGCGGTGAAGCTCAACCCCTCCTCCTCTGAGATCATGGGCAATGGCCGTGTCTCCATGAGGAAAACCACCAAGCCTGTTCCCTCCGGGAGCCCATGGTACGGACCAGACCGTGTTAAATACTTGGGCCCGTTCTCTGGTGAGCCCCCATCCTACTTGACTGGTGAGTTCCCTGGTGACTACGGCTGGGACACTGCTGGCCTCTCTGCTGACCCAGAGACCTTTGCCAAGAACCGTGAGCTTGAAGTCATCCATTCCAGGTGGGCCATGCTTGGAGCTCTTGGATGCGTCTTCCCCGAGCTCTTGTCCCGCAACGGTGTCAAGTTCGGCGAGGCTGTATGGTTCAAGGCTGGAGCCCAGATCTTCAGCGAGGGTGGACTTGACTACTTGGGCAACCCAAGCTTGATCCAC SRR13695458 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 04:31:26 Started mapping on | Feb 12 04:31:26 Finished on | Feb 12 04:33:20 Mapping speed, Million of reads per hour | 512.25 Number of input reads | 16221235 Average input read length | 292 UNIQUE READS: Uniquely mapped reads number | 15143811 Uniquely mapped reads % | 93.36% Average mapped length | 290.98 Number of splices: Total | 14269881 Number of splices: Annotated (sjdb) | 13996544 Number of splices: GT/AG | 13963298 Number of splices: GC/AG | 255155 Number of splices: AT/AC | 8953 Number of splices: Non-canonical | 42475 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.03% Deletion average length | 2.91 Insertion rate per base | 0.02% Insertion average length | 2.29 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 369557 % of reads mapped to multiple loci | 2.28% Number of reads mapped to too many loci | 54185 % of reads mapped to too many loci | 0.33% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.88% % of reads unmapped: other | 0.15% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 708049 708049 708049 N_multimapping 369557 369557 369557 N_noFeature 362884 14749636 534258 N_ambiguous 318896 1599 95173 UnstrandedReadsAssigned:14462031 PositiveStrandReadsAssigned:392576 NegativeStrandReadsAssigned:14514380 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695458 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695458-trimmed-pair1.fastq SRR13695458-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 16,221,235 reads, 14,622,012 reads pseudoaligned [quant] estimated average fragment length: 219.855 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,148 rounds 52401 SRR13695458.ke.tsv 34699 SRR13695458.se.tsv 87100 total ==> SRR13695458.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1799.15 543 15.3718 Potri.005G024800.1.v4.1 1035 816.145 469 29.2683 Potri.004G059700.1.v4.1 961 742.175 1 0.0686256 Potri.007G009000.2.v4.1 1416 1197.15 0 0 Potri.003G141000.2.v4.1 2943 2724.15 623 11.648 Potri.016G087400.1.v4.1 270 93.729 925.291 502.801 Potri.015G069301.1.v4.1 564 349.043 0 0 Potri.010G195200.1.v4.1 1773 1554.15 144 4.71914 Potri.012G127500.1.v4.1 977 758.17 120 8.06133 ==> SRR13695458.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 89 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 395 Potri.001G212900.v4.1 57 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 4 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 5 SRR13695458 completed mapping pipeline successfully