Starting /dee2/code/volunteer_pipeline.sh SRR13695459 current disk space = 3049123414016 free memory = 1579174388 SRR13695459 SRAfilesize fae8b11691cda4d9156dea1427a20c2b SRR13695459.sra SRR13695459.sra file validated SRR13695459 is paired end SRR13695459 is conventional basespace SRR13695459 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695459_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.589 37.0 37.0 37.0 37.0 37.0 2 36.3335 37.0 37.0 37.0 37.0 37.0 3 36.5895 37.0 37.0 37.0 37.0 37.0 4 36.6545 37.0 37.0 37.0 37.0 37.0 5 36.6355 37.0 37.0 37.0 37.0 37.0 6 36.667 37.0 37.0 37.0 37.0 37.0 7 36.504 37.0 37.0 37.0 37.0 37.0 8 36.5785 37.0 37.0 37.0 37.0 37.0 9 36.536 37.0 37.0 37.0 37.0 37.0 10-14 36.569900000000004 37.0 37.0 37.0 37.0 37.0 15-19 36.568000000000005 37.0 37.0 37.0 37.0 37.0 20-24 36.5222 37.0 37.0 37.0 37.0 37.0 25-29 36.4739 37.0 37.0 37.0 37.0 37.0 30-34 36.4575 37.0 37.0 37.0 37.0 37.0 35-39 36.4402 37.0 37.0 37.0 37.0 37.0 40-44 36.4628 37.0 37.0 37.0 37.0 37.0 45-49 36.422399999999996 37.0 37.0 37.0 37.0 37.0 50-54 36.3856 37.0 37.0 37.0 37.0 37.0 55-59 36.3966 37.0 37.0 37.0 37.0 37.0 60-64 36.4184 37.0 37.0 37.0 37.0 37.0 65-69 36.3513 37.0 37.0 37.0 37.0 37.0 70-74 36.342 37.0 37.0 37.0 37.0 37.0 75-79 36.3317 37.0 37.0 37.0 37.0 37.0 80-84 36.196999999999996 37.0 37.0 37.0 37.0 37.0 85-89 36.2491 37.0 37.0 37.0 37.0 37.0 90-94 36.2094 37.0 37.0 37.0 37.0 37.0 95-99 36.1983 37.0 37.0 37.0 37.0 37.0 100-104 36.171 37.0 37.0 37.0 37.0 37.0 105-109 36.16329999999999 37.0 37.0 37.0 37.0 37.0 110-114 36.1397 37.0 37.0 37.0 37.0 37.0 115-119 36.1207 37.0 37.0 37.0 37.0 37.0 120-124 35.9944 37.0 37.0 37.0 37.0 37.0 125-129 35.9397 37.0 37.0 37.0 37.0 37.0 130-134 35.9842 37.0 37.0 37.0 37.0 37.0 135-139 35.91420000000001 37.0 37.0 37.0 37.0 37.0 140-144 35.8584 37.0 37.0 37.0 37.0 37.0 145-149 35.619299999999996 37.0 37.0 37.0 37.0 37.0 150-151 35.441 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 1.0 25 5.0 26 1.0 27 11.0 28 13.0 29 18.0 30 26.0 31 29.0 32 53.0 33 71.0 34 117.0 35 332.0 36 2941.0 37 382.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.525 10.45 8.725 41.3 2 20.84379708689101 13.962832747363136 36.06228026117529 29.13108990457057 3 20.025000000000002 17.974999999999998 27.575 34.425 4 23.025000000000002 24.525 23.825 28.625 5 23.35 32.9 22.525000000000002 21.224999999999998 6 20.775 34.8 23.849999999999998 20.575 7 15.6 28.125 42.05 14.224999999999998 8 18.05 27.0 31.225 23.724999999999998 9 18.95 24.775 34.849999999999994 21.425 10-14 19.335 30.165 27.565 22.935 15-19 19.36 28.694999999999997 27.500000000000004 24.445 20-24 20.13 28.845 27.85 23.175 25-29 19.77 29.465000000000003 27.944999999999997 22.82 30-34 19.655 29.32 27.6 23.425 35-39 19.695 29.015 27.689999999999998 23.599999999999998 40-44 20.505000000000003 29.75 26.86 22.884999999999998 45-49 19.7 29.13 27.450000000000003 23.72 50-54 20.505000000000003 29.265 26.97 23.26 55-59 20.015 30.195 27.16 22.63 60-64 19.805 28.99 27.875 23.330000000000002 65-69 20.405 29.395 26.384999999999998 23.815 70-74 20.424999999999997 29.13 26.955000000000002 23.49 75-79 20.215 29.375 26.93 23.48 80-84 20.735 27.82 27.994999999999997 23.45 85-89 20.34 29.28 27.37 23.01 90-94 20.665 27.935 27.515 23.885 95-99 20.615 28.075 27.189999999999998 24.12 100-104 19.950000000000003 28.77 27.800000000000004 23.48 105-109 20.645 28.249999999999996 27.884999999999998 23.22 110-114 20.11 28.77 28.084999999999997 23.035 115-119 20.915 29.015 26.450000000000003 23.62 120-124 20.22 29.455 27.339999999999996 22.985 125-129 20.365 28.98 26.51 24.145 130-134 20.945 29.104999999999997 27.415 22.535 135-139 20.919999999999998 28.634999999999998 26.855 23.59 140-144 20.835 27.74 27.47 23.955000000000002 145-149 20.635 28.275 27.115000000000002 23.974999999999998 150-151 21.0125 27.85 26.387500000000003 24.75 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.5 15 0.5 16 0.5 17 0.5 18 0.0 19 1.0 20 1.5 21 1.5 22 1.5 23 1.0 24 2.5 25 3.5 26 6.0 27 9.0 28 11.0 29 13.0 30 26.0 31 42.5 32 40.0 33 41.5 34 56.5 35 76.5 36 100.5 37 104.0 38 129.0 39 178.5 40 191.5 41 192.0 42 237.0 43 264.5 44 248.0 45 241.0 46 258.5 47 242.5 48 204.5 49 210.5 50 189.0 51 158.5 52 127.0 53 93.0 54 76.0 55 59.5 56 48.5 57 33.5 58 25.0 59 17.5 60 7.0 61 5.0 62 8.0 63 8.0 64 3.5 65 0.5 66 0.0 67 0.0 68 0.0 69 0.0 70 0.5 71 0.5 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.44999999999999996 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.5 #Duplication Level Percentage of deduplicated Percentage of total 1 68.53383458646617 45.574999999999996 2 20.112781954887218 26.75 3 6.616541353383458 13.200000000000001 4 2.8947368421052633 7.7 5 1.0902255639097744 3.6249999999999996 6 0.6015037593984963 2.4 7 0.11278195488721805 0.525 8 0.0 0.0 9 0.03759398496240602 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTTAGACACAGGTACCTCACCCTTAGATGGCAACTGCATGGACCCAGAA 9 0.22499999999999998 No Hit ACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACC 7 0.17500000000000002 No Hit ATTCAGAACTCAGATGTCTTTTTTGCTCTCTTTTTTTTCCCTTCCAATTA 7 0.17500000000000002 No Hit TACTGGTGGTACCTATAATCAGGTTTTGTTGGCTCATTCTGAAAGAGAGT 7 0.17500000000000002 No Hit GGCAGGTAAGCTCACAGCCCAAAGGACAAGAAAACAACTGAACAGCCATC 6 0.15 No Hit GTGGAGTCAAAAACAAAACAAACGCAAAAGATGCAGGTGCAAATGCAGAT 6 0.15 No Hit GTGGGGGCTTCCGTGGAAACGGAGCATTTTATCGGAGAGTGAAATGAAAA 6 0.15 No Hit GTGGCATTGGGATCTTTAGGCATGAGCATTTCTTTCCCAACTGGTCCTGT 6 0.15 No Hit GGGGGTTTGAGATTGGCTCAGCTGATATGCCCCCATAATCTGAGGTAAAC 6 0.15 No Hit GAGTTCTGTCAATGATTTGAAGCCGAAAGGGTCTAACTTGAATGTGTTCA 6 0.15 No Hit GTCTGGTTTAGGGCATGGAGGGTAGTTGCTGACCTTGGTTCCAAAAGTCG 6 0.15 No Hit GTAAGCTTTCTTTGCCTCCTCAAGCTCAAGCAACACTTGAGATGCCTCAG 6 0.15 No Hit CGCAGTTTCATTTGAGACTACAAATGAAGGAAGAAAAACTTGGGACAAAT 6 0.15 No Hit TGTGTCTTGCCTAAAGAAACCGAGTCCTTAAACCTACGGACTATAGTTGA 6 0.15 No Hit CCCAAAGCCAAGCATCACAGCCTGCATCCCTTGCACCCCATAAATCATTC 6 0.15 No Hit CCCCTGTCATAAACCACTTTAGCATCCATCACCTTCCCTTTCTCTCTAAA 6 0.15 No Hit GCTGAGAGAGGAGACAGCTGCGGGAGAGAGCAGATAGAGTAGAGAACTGG 6 0.15 No Hit CCACCATTTCTAATTTTCTTTCAAACCAAAAATCTGGACACACTTAATAC 6 0.15 No Hit AGAGCATCTTTGCACGGATTCGAGAGGTTGAAGGAGACCATGCAATGAAG 6 0.15 No Hit AGCGATTATTTGCAACATTGTCATATACAAAATCCCTCAAGAACAGAGGC 6 0.15 No Hit TACTGGTATTGTAGCCAAAGTAGGAAAACAACCTGCTAACGGATTAACTC 5 0.125 No Hit CTCTTTTCTAGTATGCAACCGATACCCATCCAAAGGCGGCAATAGCTGGT 5 0.125 No Hit GTTGATTTCAAAAATGGAAGGCTCTTTAAAACAAGCGGCAGAATTTTTTC 5 0.125 No Hit CTTTGAAAATGATGTCGTGGAATAAGCAGAGATGACTCACAATTGTTGGT 5 0.125 No Hit CTTCTGTCTCCGACAACACTTCGTACTTCAGCTTGTGCTTTCTCCATGAC 5 0.125 No Hit GTTCCTTGGCCTCCCTGGCCCAGCGTTCTATTGACATTGTAATGATCAGT 5 0.125 No Hit GGGCAACCTCAGCATCACAAAGAACTGACAATTCAAAAGCCTTCTTCAGC 5 0.125 No Hit GCTAGATATTTTACGAGTAGACAGGAGGGTACAAGTGTTCTAAAAGTAAT 5 0.125 No Hit GTAGGAGTACTACAGGGCCGTGCCTGTTTGATATCTTGGATAGGGATCGG 5 0.125 No Hit CGTATTTACATGGGCAACCTTGGTTGAAGGCAACCTCATCAACGATGCTC 5 0.125 No Hit GTTTGGTTCTAAGAGCACAAACTGCTCCTTCAGATAAAATAGCTCATCCA 5 0.125 No Hit CTCCGAGCGATCCCAAGCCCCAAACGAAATCTAGCCAGAGCATTTTATTT 5 0.125 No Hit CTGGAACACCATCACTAACTAGAAGAGTAATCATTCCATAATCTGAATGT 5 0.125 No Hit GTTGTTGATATAGCCATGAGAATCCCACGGTTGGCACATGGTCCTCTACA 5 0.125 No Hit GGTGACTTCTCCATAGAACTTGGTAGAGATGTTACTTAGATGGCTTTCGA 5 0.125 No Hit GACAGGAGCTGCTTGACCTGACCCACGACTTTGCGACCTATCTGCATAAA 5 0.125 No Hit CAGGAATTTTCTGTGCCATGTCAAGACCACATATTCACATATACCACAAG 5 0.125 No Hit CTGTTATTCCTGCCTCCTGATACATATTTTCAAGCTGCTCCTTGGCCTGA 5 0.125 No Hit CTCAGATTTACTAGCTAAAAGCTCTTCAAACTGTTTCAAGAGCGCTGGTG 5 0.125 No Hit GCATGAAGAAGCATCCACAATATTGTATGAAGGACTGATCAGTATTGGAT 5 0.125 No Hit GCAACAATTCCATCACTTTGAAGCAACTTGCACCCCTGATGATGCTCTAA 5 0.125 No Hit GTGCCAATGCCTCATCATTGAAAAAAGAAGAAGAAAAACTGTGCACGATT 5 0.125 No Hit CATCAACATTTTCCTCAATGAGCTTATCACTATTAGCTGGCCCTAGTGGT 5 0.125 No Hit GTCTGGCCACCCTATCTGTATCTTGTGATTATACATCCTGCAGCTGACCA 5 0.125 No Hit CTCCATTGCCATATTTATTTCTGTTCAGGATGTTGATGGCTTCTTCAACG 5 0.125 No Hit GGGGTCAAGTCCTTCGCCAAGTGGCCCTCCACCCACTCTGTACCCTTCAA 5 0.125 No Hit GTCGAGGATACCAGTAACATTCAGATGAAAATAGTTCTGGTATGTTTTGA 5 0.125 No Hit CTCTTTATCTCCAACAGCGCGTCCCTTTGCCTTGCGATCCAACAAAGACT 5 0.125 No Hit CGTCAAAGAGCGCACACCCCTTAGAAGTTCTAGAGATCTCAAGGCAGATG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0125 0.0 0.0 0.0 0.0 74-75 0.0625 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.15000000000000002 0.0 0.0 0.0 0.0 80-81 0.225 0.0 0.0 0.0 0.0 82-83 0.3625 0.0 0.0 0.0 0.0 84-85 0.4375 0.0 0.0 0.0 0.0 86-87 0.475 0.0 0.0 0.0 0.0 88-89 0.5 0.0 0.0 0.0 0.0 90-91 0.5874999999999999 0.0 0.0 0.0 0.0 92-93 0.6 0.0 0.0 0.0 0.0 94-95 0.675 0.0 0.0 0.0 0.0 96-97 0.8625 0.0 0.0 0.0 0.0 98-99 1.1375 0.0 0.0 0.0 0.0 100-101 1.3875000000000002 0.0 0.0 0.0 0.0 102-103 1.5 0.0 0.0 0.0 0.0 104-105 2.0 0.0 0.0 0.0 0.0 106-107 2.1500000000000004 0.0 0.0 0.0 0.0 108-109 2.4875 0.0 0.0 0.0 0.0 110-111 2.6375 0.0 0.0 0.0 0.0 112-113 2.9875 0.0 0.0 0.0 0.0 114-115 3.55 0.0 0.0 0.0 0.0 116-117 3.975 0.0 0.0 0.0 0.0 118-119 4.2625 0.0 0.0 0.0 0.0 120-121 4.675000000000001 0.0 0.0 0.0 0.0 122-123 5.275 0.0 0.0 0.0 0.0 124-125 5.824999999999999 0.0 0.0 0.0 0.0 126-127 6.225 0.0 0.0 0.0 0.0 128-129 6.65 0.0 0.0 0.0 0.0 130-131 7.3375 0.0 0.0 0.0 0.0 132-133 7.7875 0.0 0.0 0.0 0.0 134-135 8.4 0.0 0.0 0.0 0.0 136-137 8.850000000000001 0.0 0.0 0.0 0.0 138-139 9.7375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR13695459 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695459_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.21375 37.0 37.0 37.0 37.0 37.0 2 36.17 37.0 37.0 37.0 37.0 37.0 3 36.282 37.0 37.0 37.0 37.0 37.0 4 36.3695 37.0 37.0 37.0 37.0 37.0 5 36.267 37.0 37.0 37.0 37.0 37.0 6 36.282 37.0 37.0 37.0 37.0 37.0 7 36.2285 37.0 37.0 37.0 37.0 37.0 8 36.31 37.0 37.0 37.0 37.0 37.0 9 36.2805 37.0 37.0 37.0 37.0 37.0 10-14 36.3264 37.0 37.0 37.0 37.0 37.0 15-19 36.290800000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.312799999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.2716 37.0 37.0 37.0 37.0 37.0 30-34 36.2487 37.0 37.0 37.0 37.0 37.0 35-39 36.2082 37.0 37.0 37.0 37.0 37.0 40-44 36.2173 37.0 37.0 37.0 37.0 37.0 45-49 36.1902 37.0 37.0 37.0 37.0 37.0 50-54 36.1554 37.0 37.0 37.0 37.0 37.0 55-59 36.0801 37.0 37.0 37.0 37.0 37.0 60-64 35.985699999999994 37.0 37.0 37.0 37.0 37.0 65-69 36.057500000000005 37.0 37.0 37.0 37.0 37.0 70-74 36.0291 37.0 37.0 37.0 37.0 37.0 75-79 36.0461 37.0 37.0 37.0 37.0 37.0 80-84 36.041000000000004 37.0 37.0 37.0 37.0 37.0 85-89 35.9245 37.0 37.0 37.0 37.0 37.0 90-94 35.9265 37.0 37.0 37.0 37.0 37.0 95-99 35.904700000000005 37.0 37.0 37.0 37.0 37.0 100-104 35.863 37.0 37.0 37.0 37.0 37.0 105-109 35.895 37.0 37.0 37.0 37.0 37.0 110-114 35.7503 37.0 37.0 37.0 37.0 37.0 115-119 35.7125 37.0 37.0 37.0 37.0 37.0 120-124 35.66010000000001 37.0 37.0 37.0 37.0 37.0 125-129 35.67895 37.0 37.0 37.0 37.0 37.0 130-134 35.5744 37.0 37.0 37.0 37.0 37.0 135-139 35.5923 37.0 37.0 37.0 37.0 37.0 140-144 35.3423 37.0 37.0 37.0 34.6 37.0 145-149 35.165800000000004 37.0 37.0 37.0 29.8 37.0 150-151 34.9765 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 0.0 15 5.0 16 1.0 17 1.0 18 0.0 19 1.0 20 1.0 21 1.0 22 3.0 23 9.0 24 3.0 25 5.0 26 8.0 27 12.0 28 19.0 29 10.0 30 28.0 31 29.0 32 57.0 33 88.0 34 175.0 35 547.0 36 2779.0 37 217.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.826272248683885 21.509150162948107 14.36450238154926 27.300075206818754 2 28.050000000000004 26.325 29.525000000000002 16.1 3 20.3 28.775000000000002 31.974999999999998 18.95 4 25.6 34.575 22.0 17.825 5 25.174999999999997 35.125 22.5 17.2 6 20.3 39.900000000000006 22.675 17.125 7 20.175 21.55 38.550000000000004 19.725 8 21.325 26.174999999999997 28.775000000000002 23.724999999999998 9 22.875 23.375 32.125 21.625 10-14 24.015 29.515 26.884999999999998 19.585 15-19 23.1 28.804999999999996 27.675 20.419999999999998 20-24 22.882288228822883 28.682868286828683 28.18781878187819 20.24702470247025 25-29 23.34167083541771 28.53926963481741 28.039019509754876 20.080040020010003 30-34 22.614522904580916 28.675735147029407 28.270654130826166 20.43908781756351 35-39 23.03230323032303 28.03780378037804 27.2977297729773 21.632163216321633 40-44 23.084616923384676 28.380676135227045 27.850570114022805 20.684136827365474 45-49 22.817281728172816 28.642864286428644 28.252825282528253 20.287028702870288 50-54 23.235 28.050000000000004 28.315 20.4 55-59 22.77638819409705 27.94897448724362 28.3991995997999 20.87543771885943 60-64 23.352335233523352 27.28772877287729 28.5028502850285 20.857085708570857 65-69 23.09 27.474999999999998 28.7 20.735 70-74 23.371685842921462 27.18359179589795 28.264132066033014 21.180590295147574 75-79 23.38701610483145 27.13814144243273 28.39351805541662 21.081324397319197 80-84 23.494999999999997 28.185 27.150000000000002 21.17 85-89 23.95958383353341 28.036214485794318 27.25090036014406 20.753301320528212 90-94 24.51745174517452 27.477747774777477 27.71777177717772 20.287028702870288 95-99 23.53 28.754999999999995 27.860000000000003 19.855 100-104 23.736868434217108 27.743871935967984 27.698849424712357 20.82041020510255 105-109 23.97959183673469 27.986194477791116 27.47098839535814 20.563225290116048 110-114 23.462346234623464 28.15781578157816 27.947794779477945 20.432043204320433 115-119 23.897169150745224 28.008402520756228 28.093428028408525 20.001000300090027 120-124 24.917458729364682 28.224112056028016 27.153576788394197 19.704852426213108 125-129 24.191047761940485 28.132033008252062 27.16679169792448 20.510127531882972 130-134 24.052026013006504 27.99899949974988 28.129064532266135 19.81990995497749 135-139 25.26263131565783 27.61880940470235 27.423711855927962 19.694847423711856 140-144 25.46254625462546 28.26282628262826 26.697669766976695 19.576957695769575 145-149 27.623811905952977 26.898449224612307 27.27863931965983 18.19909954977489 150-151 27.851425712856425 27.963981990995496 25.46273136568284 18.721860930465233 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 2.0 14 1.5 15 0.0 16 0.0 17 0.5 18 1.5 19 1.0 20 1.0 21 1.0 22 0.0 23 1.0 24 3.5 25 3.0 26 2.5 27 5.5 28 4.5 29 13.5 30 23.0 31 22.0 32 27.0 33 36.5 34 52.5 35 82.0 36 105.0 37 101.5 38 135.5 39 177.5 40 192.0 41 241.5 42 282.0 43 265.5 44 247.0 45 251.0 46 264.5 47 269.0 48 242.0 49 189.0 50 141.0 51 124.0 52 109.0 53 85.5 54 64.5 55 59.0 56 46.5 57 30.5 58 20.0 59 14.5 60 17.5 61 11.0 62 8.0 63 7.0 64 2.5 65 0.0 66 0.5 67 1.0 68 0.5 69 0.0 70 0.0 71 0.5 72 0.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27499999999999997 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.01 25-29 0.05 30-34 0.02 35-39 0.01 40-44 0.02 45-49 0.01 50-54 0.0 55-59 0.05 60-64 0.01 65-69 0.0 70-74 0.05 75-79 0.03 80-84 0.0 85-89 0.04 90-94 0.01 95-99 0.0 100-104 0.05 105-109 0.04 110-114 0.01 115-119 0.03 120-124 0.05 125-129 0.025 130-134 0.05 135-139 0.05 140-144 0.01 145-149 0.05 150-151 0.05 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.8 #Duplication Level Percentage of deduplicated Percentage of total 1 69.08682634730539 46.150000000000006 2 19.947604790419163 26.650000000000002 3 6.362275449101797 12.75 4 2.769461077844311 7.3999999999999995 5 1.0104790419161676 3.375 6 0.561377245508982 2.25 7 0.14970059880239522 0.7000000000000001 8 0.037425149700598806 0.2 9 0.0 0.0 >10 0.07485029940119761 0.525 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATC 11 0.27499999999999997 No Hit AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 10 0.25 No Hit GGAAGCTCAGAAGGTAGATTAAAAAACTAAAGAGGAAGAATGTGGATGAG 8 0.2 No Hit TAATGATTTTGCTGGAAAGGATCAGACAATCTCATTGGAAGAAATCAGGG 7 0.17500000000000002 No Hit CATAATTGTTAGCACTAGACCCCATCTTCCATCCATCATTGAACACATTC 7 0.17500000000000002 No Hit GTTGAAGGCTTCTCACATGTCATGTTTGCCACTGGACGTCGGCCTAATAC 7 0.17500000000000002 No Hit CAAAACCACATATAGAGGGTGTAATAGCTAAGTAGCCTGTAAGAGATGGC 7 0.17500000000000002 No Hit GGAAAATCTTCTGCTTTCATCAAAGCTGCTGTTCTGTGTCATCCTTCATT 6 0.15 No Hit AGTTTGCACTGGAATTGGAAGGACTCGCCGAGCAACTTTTAGACTTGCTG 6 0.15 No Hit TGTCAATTCTCGAGAAATATCATGTGAAATGGTTTTGGATCCTGATCCAA 6 0.15 No Hit AAGCGGGTGTAAAATTGGCTGTTGTGTCAAACTTTGACACTCGTCTTAGA 6 0.15 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 6 0.15 No Hit TCATTTCTCTTCCACACTCACTGCGAGAGAGAAATGGAAATGGCAATTAA 6 0.15 No Hit ATGGGATGTGATTGTCTCCTCCCTCTCTGCTCACTCGATCTCCCCGGCCC 6 0.15 No Hit AGTTGAGAGAGATAGATCACATATCAAGTCGGAAGCTGTGTTGAAGATAA 6 0.15 No Hit AAACAGGCTGCAGGTCATACAGGCAATATTATGCATGCCAATATGTTGCC 6 0.15 No Hit CCCTCCCCAGAGGGAAACTTCCTTCTCAAGACTTCCCCAGAGGGAAAATT 6 0.15 No Hit CTTGAAGGAAGGAAACTTGATTTGCTGAAATGGCTGATAACAATAACAAT 6 0.15 No Hit AGTGATTCCAGATGGAATTGATAAGAATGGGAAGCCTCATAAACTAAGAT 6 0.15 No Hit CAGCAAAATAGCATTCGTGGAACTTCAGAGGAAGAAGGTCCATCGGTATG 6 0.15 No Hit GTTCCTTGCTTGGAATTCGAGTTGGAGAAAGGTTGGGTCTACCGTGAGCA 6 0.15 No Hit ATCCTACGTCTGATGTTGTCATCGAGACACACAAAGAGGAAGAGTTTAGC 6 0.15 No Hit TTTCTCTCGATGTTGAGTGGAATATCAAAGCACCTTTTCGGTGCATATCC 5 0.125 No Hit CTTAGGTAATGAAGCCTGGAAAAATATTAATGGATTCTTTTCTTGGGAAA 5 0.125 No Hit GTACAAAGTATTCAAGAGAAAGAGGAGTGAAAAACTAAAGAAAAAGTACT 5 0.125 No Hit TGCCTATTTGCATTTACTCTCTTGCATCCTTAAATCAATCCATCAAGACA 5 0.125 No Hit CATCAGCAATATTCTTTTAGCTCCCTTCAAAAGCTGGAGGGAGTTCGGAA 5 0.125 No Hit GTCTGTACTGGAAATGGATGGGCAATTTGACAGATTAGATGAGCTCATCT 5 0.125 No Hit CAGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 5 0.125 No Hit TGAAACTCCCTGAATCAAGACGCCAAATTGCAGAATTTGAATCGATGTTG 5 0.125 No Hit GTTCCCATGTTTTGGCTAGTGCTTATAGTGGTGGTCATGGGAGAGGCATT 5 0.125 No Hit AATTGTTCAAGGCCCCCCAGGCAGTTGATTATAATGATGGAATATCAGTT 5 0.125 No Hit ATAGCTACTTAATTCTCTCTTTGGCTGGTGATCTTAGATCTCAGCATCAA 5 0.125 No Hit GGCAAAGCAATCTGCAAATTCTAACAAAGGCGAACCGGTTCTAAAGTGGC 5 0.125 No Hit CCAAAAACGAAGAAAACCAGAACAAGAGCGAGGCTTCTACCCCAGCTTCA 5 0.125 No Hit TTTTACTTCTCCTTTTTGCAGTTCTTGCCATTCCCCTGCTGCAGTTGTTC 5 0.125 No Hit CTACGAGGGTGCCCCATTGACAGAATACACACTGCGGCTGATTGGTTCAG 5 0.125 No Hit CTTAAATCTCGTATGTAGTAAAAAAAATGGTTAATGCTCGGTAACTGCTT 5 0.125 No Hit CGTAGCAATGTTTGGAGGAGCGTGTTCCACCCTGGTAGCAACCTTGCTAC 5 0.125 No Hit GGGACTTATAAAGGACGTGAGGGCAAGGTGGTTCAAGTTTATCGCAGAAA 5 0.125 No Hit GTGAGGCGCAGTTTAGGTCTGGAATTGAAGAGTTGATACTTGTTGTTGCC 5 0.125 No Hit CCTGCAACCTAAGATAAAAGCTATTCAACAAAGATATGCAGGAAATCAGG 5 0.125 No Hit TGACAGAGCTAGTCTGTTCTTGTATAAACAGTAAACCACCATGTTCTTGC 5 0.125 No Hit AATGGAGACTGCAATTTGTGGGAGAATAGCTCTCTCACCTAATCACGTCT 5 0.125 No Hit AAACAGGCTAAGGAGCGAGTGTGCAGATTAATTCAAAGTGGTGTTGAAAG 5 0.125 No Hit CTAGTTGATGCTTGGAAGGGCAAACCGGTATGTATCTAGTCTCTCTATAG 5 0.125 No Hit TGCTTTCTCTGTTTGGGACTCGCCTCCACCTTCTCCCAATCCCATTCCTC 5 0.125 No Hit TCTGCTGGGAAAAAATTAATCTATTTGATTGCTATGGCTTTAAATCTGGA 5 0.125 No Hit TTCTAATGAGGTTAAAGTGCCTCTGGATATACTCATACTCAAACTCATGA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0125 0.0 0.0 0.0 0.0 74-75 0.0625 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.15000000000000002 0.0 0.0 0.0 0.0 80-81 0.225 0.0 0.0 0.0 0.0 82-83 0.3625 0.0 0.0 0.0 0.0 84-85 0.4375 0.0 0.0 0.0 0.0 86-87 0.475 0.0 0.0 0.0 0.0 88-89 0.5 0.0 0.0 0.0 0.0 90-91 0.5874999999999999 0.0 0.0 0.0 0.0 92-93 0.6 0.0 0.0 0.0 0.0 94-95 0.675 0.0 0.0 0.0 0.0 96-97 0.8625 0.0 0.0 0.0 0.0 98-99 1.1375 0.0 0.0 0.0 0.0 100-101 1.3875000000000002 0.0 0.0 0.0 0.0 102-103 1.5 0.0 0.0 0.0 0.0 104-105 2.0 0.0 0.0 0.0 0.0 106-107 2.1875 0.0 0.0 0.0 0.0 108-109 2.5375 0.0 0.0 0.0 0.0 110-111 2.6875 0.0 0.0 0.0 0.0 112-113 3.0375 0.0 0.0 0.0 0.0 114-115 3.6125 0.0 0.0 0.0 0.0 116-117 4.0375 0.0 0.0 0.0 0.0 118-119 4.3625 0.0 0.0 0.0 0.0 120-121 4.800000000000001 0.0 0.0 0.0 0.0 122-123 5.375 0.0 0.0 0.0 0.0 124-125 5.925000000000001 0.0 0.0 0.0 0.0 126-127 6.325 0.0 0.0 0.0 0.0 128-129 6.725 0.0 0.0 0.0 0.0 130-131 7.4 0.0 0.0 0.0 0.0 132-133 7.8125 0.0 0.0 0.0 0.0 134-135 8.35 0.0 0.0 0.0 0.0 136-137 8.8 0.0 0.0 0.0 0.0 138-139 9.6625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 935836 spots for SRR13695459.sra Written 935836 spots for SRR13695459.sra Read 935836 spots for SRR13695459.sra Written 935836 spots for SRR13695459.sra Read 935836 spots for SRR13695459.sra Written 935836 spots for SRR13695459.sra Read 935836 spots for SRR13695459.sra Written 935836 spots for SRR13695459.sra Read 935836 spots for SRR13695459.sra Written 935836 spots for SRR13695459.sra Read 935836 spots for SRR13695459.sra Written 935836 spots for SRR13695459.sra Read 935836 spots for SRR13695459.sra Written 935836 spots for SRR13695459.sra Read 935836 spots for SRR13695459.sra Written 935836 spots for SRR13695459.sra Read 935836 spots for SRR13695459.sra Written 935836 spots for SRR13695459.sra Read 935836 spots for SRR13695459.sra Written 935836 spots for SRR13695459.sra Read 935836 spots for SRR13695459.sra Written 935836 spots for SRR13695459.sra Read 935836 spots for SRR13695459.sra Written 935836 spots for SRR13695459.sra Read 935836 spots for SRR13695459.sra Written 935836 spots for SRR13695459.sra Read 935836 spots for SRR13695459.sra Written 935836 spots for SRR13695459.sra Read 935836 spots for SRR13695459.sra Written 935836 spots for SRR13695459.sra Read 935836 spots for SRR13695459.sra Written 935836 spots for SRR13695459.sra Read 935843 spots for SRR13695459.sra Written 935843 spots for SRR13695459.sra Read 935836 spots for SRR13695459.sra Written 935836 spots for SRR13695459.sra Read 935836 spots for SRR13695459.sra Written 935836 spots for SRR13695459.sra Read 935836 spots for SRR13695459.sra Written 935836 spots for SRR13695459.sra SRR ids: ['SRR13695459.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_f7uk6o1i SRR13695459.sra spots: 18716727 blocks: [[1, 935836], [935837, 1871672], [1871673, 2807508], [2807509, 3743344], [3743345, 4679180], [4679181, 5615016], [5615017, 6550852], [6550853, 7486688], [7486689, 8422524], [8422525, 9358360], [9358361, 10294196], [10294197, 11230032], [11230033, 12165868], [12165869, 13101704], [13101705, 14037540], [14037541, 14973376], [14973377, 15909212], [15909213, 16845048], [16845049, 17780884], [17780885, 18716727]] SRR13695459 file size 6339062 SRR13695459 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695459 SRR13695459_1.fastq SRR13695459_2.fastq Input file: SRR13695459_1.fastq Paired file: SRR13695459_2.fastq trimmed: SRR13695459-trimmed-pair1.fastq, SRR13695459-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 05:06:13 2025 >> started Wed Feb 12 05:06:33 2025 >> done (19.910s) 18716727 read pairs processed; of these: 131 ( 0.00%) short read pairs filtered out after trimming by size control 1053 ( 0.01%) empty read pairs filtered out after trimming by size control 18715543 (99.99%) read pairs available; of these: 2519965 (13.46%) trimmed read pairs available after processing 16195578 (86.54%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 2 0.00% 20 1 0.00% 21 1 0.00% 22 0 0.00% 23 1 0.00% 24 2 0.00% 25 3 0.00% 26 1 0.00% 27 1 0.00% 28 1 0.00% 29 2 0.00% 30 4 0.00% 31 1 0.00% 32 8 0.00% 33 3 0.00% 34 6 0.00% 35 5 0.00% 36 2 0.00% 37 4 0.00% 38 18 0.00% 39 5 0.00% 40 11 0.00% 41 17 0.00% 42 8 0.00% 43 22 0.00% 44 19 0.00% 45 20 0.00% 46 22 0.00% 47 31 0.00% 48 32 0.00% 49 51 0.00% 50 67 0.00% 51 73 0.00% 52 75 0.00% 53 76 0.00% 54 96 0.00% 55 112 0.00% 56 131 0.00% 57 158 0.00% 58 164 0.00% 59 236 0.00% 60 240 0.00% 61 248 0.00% 62 350 0.00% 63 396 0.00% 64 464 0.00% 65 586 0.00% 66 567 0.00% 67 694 0.00% 68 783 0.00% 69 938 0.01% 70 997 0.01% 71 1277 0.01% 72 1487 0.01% 73 1702 0.01% 74 2015 0.01% 75 2175 0.01% 76 2440 0.01% 77 2693 0.01% 78 2940 0.02% 79 3480 0.02% 80 3747 0.02% 81 4407 0.02% 82 4989 0.03% 83 5631 0.03% 84 6217 0.03% 85 6826 0.04% 86 7255 0.04% 87 7799 0.04% 88 8700 0.05% 89 8958 0.05% 90 10012 0.05% 91 10868 0.06% 92 11503 0.06% 93 12567 0.07% 94 13385 0.07% 95 14296 0.08% 96 15434 0.08% 97 16306 0.09% 98 17215 0.09% 99 18219 0.10% 100 18650 0.10% 101 19414 0.10% 102 20721 0.11% 103 21207 0.11% 104 23309 0.12% 105 23899 0.13% 106 25188 0.13% 107 25784 0.14% 108 26910 0.14% 109 27879 0.15% 110 28468 0.15% 111 29627 0.16% 112 30851 0.16% 113 31566 0.17% 114 33334 0.18% 115 34269 0.18% 116 35315 0.19% 117 36580 0.20% 118 37875 0.20% 119 39083 0.21% 120 40241 0.22% 121 41711 0.22% 122 42324 0.23% 123 42846 0.23% 124 44545 0.24% 125 45263 0.24% 126 47556 0.25% 127 46756 0.25% 128 48467 0.26% 129 49375 0.26% 130 51226 0.27% 131 51081 0.27% 132 51824 0.28% 133 53492 0.29% 134 54002 0.29% 135 54679 0.29% 136 56349 0.30% 137 57443 0.31% 138 57491 0.31% 139 60162 0.32% 140 60301 0.32% 141 61870 0.33% 142 62836 0.34% 143 62728 0.34% 144 64983 0.35% 145 65343 0.35% 146 66074 0.35% 147 67418 0.36% 148 68889 0.37% 149 69984 0.37% 150 70478 0.38% 151 16195578 86.54% 18715543 reads passed initial QC criterion=sequence-density sequence-density=0.34 sequence-density-rank=1 fanout-score=6.43 fanout-score-rank=5 prefix-density=0.65 prefix-fanout=3.4 sequence=TTGCAGCCATTCTCAGCACCAAAGTTCATCTCAGAGCTCTCGTAGAACATCCTAACTGGAGCTACACCAGCAATGATTGTCTGACTTGTGGTGGTCTCGGAGAAACTCAAGTCTGGGTACATGCTGCATCCATTGCAGCCACTGCCGCACTTGCA criterion=fanout-score sequence-density=0.15 sequence-density-rank=21 fanout-score=20.93 fanout-score-rank=1 prefix-density=0.37 prefix-fanout=8.4 sequence=TTCTTTCCAATGCT criterion=sequence-density sequence-density=0.52 sequence-density-rank=1 fanout-score=2.30 fanout-score-rank=28 prefix-density=0.54 prefix-fanout=2.2 sequence=TGCAAGTGCGGCAGTGGCTGCAATGGATGCAGCATGTACCCAGACTTGAGTTTCTCCGAGACCACCACAAGTCAGACAATCATTGCTGGTGTAGCTCCAGTTAGGATGTTCTACGAGAGCTCTGAGATGAACTTTGGTGCTGAGAATGGCTG criterion=fanout-score sequence-density=0.08 sequence-density-rank=37 fanout-score=37.50 fanout-score-rank=1 prefix-density=0.28 prefix-fanout=10.2 sequence=AAGGCCAAGATCCAGGACAAGGA SRR13695459 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 05:07:16 Started mapping on | Feb 12 05:07:16 Finished on | Feb 12 05:09:55 Mapping speed, Million of reads per hour | 423.75 Number of input reads | 18715543 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 17264959 Uniquely mapped reads % | 92.25% Average mapped length | 293.96 Number of splices: Total | 16761693 Number of splices: Annotated (sjdb) | 16370853 Number of splices: GT/AG | 16413735 Number of splices: GC/AG | 256965 Number of splices: AT/AC | 10967 Number of splices: Non-canonical | 80026 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.03% Deletion average length | 2.87 Insertion rate per base | 0.02% Insertion average length | 2.35 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 471177 % of reads mapped to multiple loci | 2.52% Number of reads mapped to too many loci | 75154 % of reads mapped to too many loci | 0.40% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.71% % of reads unmapped: other | 0.12% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 979640 979640 979640 N_multimapping 471177 471177 471177 N_noFeature 634880 16756242 884545 N_ambiguous 388938 2044 128394 UnstrandedReadsAssigned:16241141 PositiveStrandReadsAssigned:506673 NegativeStrandReadsAssigned:16252020 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695459 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695459-trimmed-pair1.fastq SRR13695459-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 18,715,543 reads, 16,180,261 reads pseudoaligned [quant] estimated average fragment length: 237.409 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,162 rounds 52401 SRR13695459.ke.tsv 34699 SRR13695459.se.tsv 87100 total ==> SRR13695459.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1781.59 1307 33.8665 Potri.005G024800.1.v4.1 1035 798.591 178 10.2896 Potri.004G059700.1.v4.1 961 724.654 3 0.191115 Potri.007G009000.2.v4.1 1416 1179.59 0 0 Potri.003G141000.2.v4.1 2943 2706.59 1159.55 19.7775 Potri.016G087400.1.v4.1 270 87.5298 997 525.827 Potri.015G069301.1.v4.1 564 333.798 0 0 Potri.010G195200.1.v4.1 1773 1536.59 294.89 8.85941 Potri.012G127500.1.v4.1 977 740.628 115 7.16805 ==> SRR13695459.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 101 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 269 Potri.001G212900.v4.1 33 Potri.001G182400.v4.1 1 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 9 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 1 SRR13695459 completed mapping pipeline successfully