Starting /dee2/code/volunteer_pipeline.sh SRR13695460 current disk space = 3049150599168 free memory = 1510955920 SRR13695460 SRAfilesize a0643c6d3b16713d6c36d80c86bd22e9 SRR13695460.sra SRR13695460.sra file validated SRR13695460 is paired end SRR13695460 is conventional basespace SRR13695460 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695460_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.557 37.0 37.0 37.0 37.0 37.0 2 36.299 37.0 37.0 37.0 37.0 37.0 3 36.5445 37.0 37.0 37.0 37.0 37.0 4 36.601 37.0 37.0 37.0 37.0 37.0 5 36.575 37.0 37.0 37.0 37.0 37.0 6 36.64 37.0 37.0 37.0 37.0 37.0 7 36.4035 37.0 37.0 37.0 37.0 37.0 8 36.5355 37.0 37.0 37.0 37.0 37.0 9 36.51 37.0 37.0 37.0 37.0 37.0 10-14 36.604200000000006 37.0 37.0 37.0 37.0 37.0 15-19 36.5578 37.0 37.0 37.0 37.0 37.0 20-24 36.5313 37.0 37.0 37.0 37.0 37.0 25-29 36.50279999999999 37.0 37.0 37.0 37.0 37.0 30-34 36.4639 37.0 37.0 37.0 37.0 37.0 35-39 36.46419999999999 37.0 37.0 37.0 37.0 37.0 40-44 36.4568 37.0 37.0 37.0 37.0 37.0 45-49 36.4061 37.0 37.0 37.0 37.0 37.0 50-54 36.4101 37.0 37.0 37.0 37.0 37.0 55-59 36.44259999999999 37.0 37.0 37.0 37.0 37.0 60-64 36.3966 37.0 37.0 37.0 37.0 37.0 65-69 36.3702 37.0 37.0 37.0 37.0 37.0 70-74 36.333800000000004 37.0 37.0 37.0 37.0 37.0 75-79 36.334900000000005 37.0 37.0 37.0 37.0 37.0 80-84 36.2621 37.0 37.0 37.0 37.0 37.0 85-89 36.287099999999995 37.0 37.0 37.0 37.0 37.0 90-94 36.214200000000005 37.0 37.0 37.0 37.0 37.0 95-99 36.1731 37.0 37.0 37.0 37.0 37.0 100-104 36.180899999999994 37.0 37.0 37.0 37.0 37.0 105-109 36.193400000000004 37.0 37.0 37.0 37.0 37.0 110-114 36.15560000000001 37.0 37.0 37.0 37.0 37.0 115-119 36.131600000000006 37.0 37.0 37.0 37.0 37.0 120-124 36.0129 37.0 37.0 37.0 37.0 37.0 125-129 35.995799999999996 37.0 37.0 37.0 37.0 37.0 130-134 36.043 37.0 37.0 37.0 37.0 37.0 135-139 35.9704 37.0 37.0 37.0 37.0 37.0 140-144 35.875099999999996 37.0 37.0 37.0 37.0 37.0 145-149 35.785900000000005 37.0 37.0 37.0 37.0 37.0 150-151 35.46875 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 1.0 25 3.0 26 6.0 27 7.0 28 8.0 29 21.0 30 20.0 31 39.0 32 47.0 33 59.0 34 128.0 35 322.0 36 2960.0 37 379.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.125 12.45 7.9 40.525 2 20.592667001506783 12.656956303365144 37.24259166248117 29.50778503264691 3 17.925 17.424999999999997 26.575 38.074999999999996 4 22.05 26.85 22.925 28.175 5 23.65 30.925000000000004 23.724999999999998 21.7 6 20.3 33.825 25.724999999999998 20.150000000000002 7 15.925 27.275 40.425 16.375 8 19.05 27.375 30.625000000000004 22.95 9 16.075 25.6 34.4 23.925 10-14 20.5 29.395 27.134999999999998 22.97 15-19 20.325 28.075 27.57 24.03 20-24 20.200000000000003 28.685 28.115000000000002 23.0 25-29 20.075000000000003 27.994999999999997 28.084999999999997 23.845 30-34 19.86 28.99 26.939999999999998 24.21 35-39 20.11 29.15 26.97 23.77 40-44 20.119999999999997 28.599999999999998 26.875 24.404999999999998 45-49 20.255000000000003 27.860000000000003 27.950000000000003 23.935000000000002 50-54 20.375 29.099999999999998 27.595 22.93 55-59 20.135 28.794999999999998 27.744999999999997 23.325000000000003 60-64 20.68 28.595 27.455000000000002 23.27 65-69 20.685000000000002 27.715 28.235 23.365 70-74 20.685000000000002 27.765 27.655 23.895 75-79 20.94 27.58 27.685 23.794999999999998 80-84 21.455 28.139999999999997 27.235 23.169999999999998 85-89 20.775 28.994999999999997 26.96 23.27 90-94 20.225 28.134999999999998 27.91 23.73 95-99 21.065 28.04 27.055 23.84 100-104 21.19 28.265 26.974999999999998 23.57 105-109 20.53 28.46 27.705000000000002 23.305 110-114 20.74 28.515 26.540000000000003 24.205 115-119 20.875 27.925 27.665 23.535 120-124 21.415 28.084999999999997 27.0 23.5 125-129 21.349999999999998 28.505000000000003 26.290000000000003 23.855 130-134 20.415 28.945 27.165 23.474999999999998 135-139 21.915000000000003 27.785 26.314999999999998 23.985 140-144 21.625 28.18 26.625 23.57 145-149 22.02 28.1 26.245 23.635 150-151 21.7875 27.187499999999996 26.5375 24.4875 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.0 21 0.0 22 0.0 23 0.0 24 1.5 25 3.0 26 6.5 27 7.0 28 13.0 29 15.5 30 18.0 31 27.5 32 34.0 33 37.0 34 54.5 35 82.5 36 90.0 37 95.0 38 119.5 39 152.0 40 163.5 41 187.0 42 229.5 43 238.0 44 251.5 45 271.5 46 252.5 47 237.5 48 244.0 49 244.0 50 197.5 51 145.5 52 136.0 53 121.0 54 86.0 55 56.5 56 47.0 57 44.0 58 31.0 59 21.0 60 15.0 61 8.0 62 3.5 63 0.5 64 2.0 65 4.5 66 2.5 67 0.0 68 0.0 69 0.0 70 0.5 71 0.5 72 0.0 73 0.0 74 0.5 75 0.5 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.44999999999999996 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.525 #Duplication Level Percentage of deduplicated Percentage of total 1 67.64593666539488 44.324999999999996 2 19.954215948111408 26.150000000000002 3 7.706982067913009 15.15 4 2.823349866463182 7.3999999999999995 5 1.06829454406715 3.5000000000000004 6 0.5723006486074018 2.25 7 0.07630675314765357 0.35000000000000003 8 0.07630675314765357 0.4 9 0.03815337657382679 0.22499999999999998 >10 0.03815337657382679 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCACCAACATTATCACCTACATTGTCAGCGATCACAGCAGGATTTCTTG 10 0.25 No Hit CTGCGTTTGGTAGGGACTTGAGGAGGCCTACTTTGGATTTGTCCCCCAAA 9 0.22499999999999998 No Hit GTGCAGGTGAGGTTGCGGTCACCATAGACATTGTCGACACGACCTGTACT 8 0.2 No Hit ACTAGTTCATCCATTCACATAAATATTACATTTTGAATTGTAAAACGAAG 8 0.2 No Hit CATAGATGCAGGGTTAATTGATGGCTCTTCACCCTTCTGATCGTCCTTGA 7 0.17500000000000002 No Hit CTCCTATCCAGCCCATTCTCAAAGAGAACGCCAACAACAACATCAGAAAC 7 0.17500000000000002 No Hit CTCAGAATCATCAAGGAAATGTTTTTCTTTAAGAGCCCTCTCACAAGCTT 6 0.15 No Hit GCACCATCAGAGGCCTCCTGGAAAGTTAATGGAGAAACATCTGACCACTT 6 0.15 No Hit ACCACCACCACCACCACCGTCGCCGCCTCCGCCTCCGCCGCTAATATCTT 6 0.15 No Hit GCGGTTGTTTTCTAATTTTCCAGTAGCCCACGAAAATGCCTGAGCTCCAA 6 0.15 No Hit GTCGTTCCCTCCTGTCAGTCCCACGCGAAGCCTCAATGAGATTAATCATT 6 0.15 No Hit ATCCGTCTCCTGCCACCTTTATTCTTTCCAAATCAATCATTATTGTCACA 6 0.15 No Hit GTTCAGTGAGTATTTCTTGCAGGGTATAGATTGGTTGTTGCTATAAACAT 6 0.15 No Hit GCCTTGGGGTCAGAAGATGAGGCGGTTTGTAATTCAGCTGTTATCTGCTC 6 0.15 No Hit GGAGCATTAGCAGGGAGCTGCAGATAGTTTGGTCCAAGACGGTGCCTCTG 6 0.15 No Hit GCTCATTGTTACTGGCATCCTTGTCTTGCTTCAACTCTTCGTCCTGTTCC 6 0.15 No Hit GTCCTGTCCTTATTGTAGCATAAATCTTAAACACCAAAAGCACAATGTTG 6 0.15 No Hit GTGCAACATCAAGTCCAGCTTTTACCAGCCCCCCCGGATTGTTTCGTAGA 6 0.15 No Hit GGTTGCCCAAGTAGTCAAGTCCACCCTCGCTGAAGATCTGGGCCCCAGCC 6 0.15 No Hit CTACTGTGCCAAAGCACTTGTGCTGTAAACAAGAAGTGCACCTCCGGCAA 6 0.15 No Hit CTCGGAGTACAGTCCCGGATTCTTGTCATATTTCTCCTTCTTGAAGTGAA 6 0.15 No Hit CTCAACTTTGCTTGCTTCTAATCTTAAGGGCGCCCACAATTACGCTTGTA 5 0.125 No Hit CTGCCACCTAAGCCACCAACTATTCTTGCAAAAAGTTGCTGCTTGGAGAT 5 0.125 No Hit GCCATCGTTCTCAGCTGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCA 5 0.125 No Hit AGCGGTGTTTTTTGGGAATTATTTTGAACTTCCGGTCGTAACTTTTCTCC 5 0.125 No Hit CCATGGAGTATGTCTTCAATCAAGGCTTCTGAAGTCTCTGGAAGGCCCTC 5 0.125 No Hit GCTATGGGCATGTCTTTTGCGATCCCGACTCCTACTTTGGCTCTGAGATA 5 0.125 No Hit CCAGTATATCTAACTAAAACCTGATAAAAACCCATATTCCATTTCATTGT 5 0.125 No Hit CCCCAACCCAAACTGCCCCTCTCTAAATCAATAAAACTCACCAGCAATTT 5 0.125 No Hit GTAGTCCACATCTCTCAACCGCCCCTTGATTATGCTTTCCATAGTTTGGA 5 0.125 No Hit GTGACTTTGGAGCAGCCCCCACATCCTTCAAAACAACAGGTTTCTTAATG 5 0.125 No Hit AGCATGTCTGGAGTCAGAAAAGCAGCAACATGGTTCACTTGCCCGTGATG 5 0.125 No Hit ATCTATTGAAAGAACCAACCACCACATTAGACTCACACAAAAACTCCAGG 5 0.125 No Hit GCTCGGGTTTCTTTGGATTGTATTCGCTCTCCCTCTATCACGCCTTGATG 5 0.125 No Hit GTACTTCTGGTCAGGGGATGGATCCATTGATGTATATACAGCTGGATTTC 5 0.125 No Hit GGGCCGTTGGGAATGCTCACAACAGTACGCCATTTGGCAAAACGAGCTCC 5 0.125 No Hit CTTTCTTCGCCTCCTCGAGCTCAATCAGCACCTGAGATGCCTCAGTGCAT 5 0.125 No Hit CAACAAAGAAAAACTAGCATCTCCCAACTATCAGATTGGAGTGAAGATAT 5 0.125 No Hit GGCACATTCAAATCAGCTCTAACAAACACTTTCTTCCCTTTCAAGTCAGC 5 0.125 No Hit CGTACGTACAGTACGGATAAGTTGATACTTTGAGCGACGGCGATCAAATG 5 0.125 No Hit CATGGGCATATCATCTTGACAAAGCTGACTGTATAGGGCTCGTAATTCAG 5 0.125 No Hit CCACACTTAAGGCCATAGACATGGCCCTTACATAACATTGAAGAAGAAGA 5 0.125 No Hit CCGAACGAAAAGCTGTGACAAAGGCCTTTCCTACACCCTTCCTTGCTTCT 5 0.125 No Hit CATTTTTCTCCCTTGAAGTTTTATAGAATTACAGAGATCAATTGTTGAAG 5 0.125 No Hit GTAACTTTTCCCACCATGCCAGCTCCCTGGTGAGGAGAACAGTAGAAGCT 5 0.125 No Hit AGCTCCTGTTGTTCTGGGATCAATTCCTTCAGCTGAGAATGAAGGTCAAG 5 0.125 No Hit ACCGTTGTCGCCCCAGTCTTCTCCCCAAGAGTTCTTGATCAGCCAATATG 5 0.125 No Hit GCGTTATCTGGGCTATGTCTCCAGCTACCAAAAAAAGCAGCACTCCAAGA 5 0.125 No Hit CTCCCCATAAAGGACATTACCGGCCATAATCCCGAAGCTTCAGTGGTGCT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.1625 0.0 0.0 0.0 0.0 76-77 0.2375 0.0 0.0 0.0 0.0 78-79 0.275 0.0 0.0 0.0 0.0 80-81 0.375 0.0 0.0 0.0 0.0 82-83 0.375 0.0 0.0 0.0 0.0 84-85 0.4375 0.0 0.0 0.0 0.0 86-87 0.5875 0.0 0.0 0.0 0.0 88-89 0.7625 0.0 0.0 0.0 0.0 90-91 0.8375 0.0 0.0 0.0 0.0 92-93 0.9750000000000001 0.0 0.0 0.0 0.0 94-95 1.1375 0.0 0.0 0.0 0.0 96-97 1.225 0.0 0.0 0.0 0.0 98-99 1.375 0.0 0.0 0.0 0.0 100-101 1.4500000000000002 0.0 0.0 0.0 0.0 102-103 1.5875 0.0 0.0 0.0 0.0 104-105 1.8 0.0 0.0 0.0 0.0 106-107 1.9875 0.0 0.0 0.0 0.0 108-109 2.1625 0.0 0.0 0.0 0.0 110-111 2.3375 0.0 0.0 0.0 0.0 112-113 2.575 0.0 0.0 0.0 0.0 114-115 2.7750000000000004 0.0 0.0 0.0 0.0 116-117 3.1624999999999996 0.0 0.0 0.0 0.0 118-119 3.5375 0.0 0.0 0.0 0.0 120-121 3.8 0.0 0.0 0.0 0.0 122-123 4.225 0.0 0.0 0.0 0.0 124-125 4.625 0.0 0.0 0.0 0.0 126-127 4.925 0.0 0.0 0.0 0.0 128-129 5.4 0.0 0.0 0.0 0.0 130-131 6.15 0.0 0.0 0.0 0.0 132-133 6.95 0.0 0.0 0.0 0.0 134-135 7.5875 0.0 0.0 0.0 0.0 136-137 8.175 0.0 0.0 0.0 0.0 138-139 8.8625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR13695460 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695460_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.0225 37.0 37.0 37.0 37.0 37.0 2 36.008 37.0 37.0 37.0 37.0 37.0 3 36.018 37.0 37.0 37.0 37.0 37.0 4 36.194 37.0 37.0 37.0 37.0 37.0 5 36.2005 37.0 37.0 37.0 37.0 37.0 6 36.158 37.0 37.0 37.0 37.0 37.0 7 36.1705 37.0 37.0 37.0 37.0 37.0 8 36.289 37.0 37.0 37.0 37.0 37.0 9 36.1905 37.0 37.0 37.0 37.0 37.0 10-14 36.1796 37.0 37.0 37.0 37.0 37.0 15-19 36.148399999999995 37.0 37.0 37.0 37.0 37.0 20-24 36.18885 37.0 37.0 37.0 37.0 37.0 25-29 36.069 37.0 37.0 37.0 37.0 37.0 30-34 36.060649999999995 37.0 37.0 37.0 37.0 37.0 35-39 36.0523 37.0 37.0 37.0 37.0 37.0 40-44 36.002449999999996 37.0 37.0 37.0 37.0 37.0 45-49 36.0496 37.0 37.0 37.0 37.0 37.0 50-54 35.971349999999994 37.0 37.0 37.0 37.0 37.0 55-59 35.969800000000006 37.0 37.0 37.0 37.0 37.0 60-64 35.914100000000005 37.0 37.0 37.0 37.0 37.0 65-69 35.9113 37.0 37.0 37.0 37.0 37.0 70-74 35.8624 37.0 37.0 37.0 37.0 37.0 75-79 35.85080000000001 37.0 37.0 37.0 37.0 37.0 80-84 35.83985 37.0 37.0 37.0 37.0 37.0 85-89 35.67475 37.0 37.0 37.0 37.0 37.0 90-94 35.7355 37.0 37.0 37.0 37.0 37.0 95-99 35.74015 37.0 37.0 37.0 37.0 37.0 100-104 35.680600000000005 37.0 37.0 37.0 37.0 37.0 105-109 35.575649999999996 37.0 37.0 37.0 37.0 37.0 110-114 35.5598 37.0 37.0 37.0 37.0 37.0 115-119 35.5016 37.0 37.0 37.0 37.0 37.0 120-124 35.4643 37.0 37.0 37.0 37.0 37.0 125-129 35.522800000000004 37.0 37.0 37.0 37.0 37.0 130-134 35.272499999999994 37.0 37.0 37.0 32.2 37.0 135-139 35.2962 37.0 37.0 37.0 32.2 37.0 140-144 35.2533 37.0 37.0 37.0 32.2 37.0 145-149 35.107 37.0 37.0 37.0 27.4 37.0 150-151 34.77525 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 2.0 14 0.0 15 0.0 16 0.0 17 1.0 18 2.0 19 1.0 20 2.0 21 2.0 22 6.0 23 5.0 24 4.0 25 7.0 26 7.0 27 12.0 28 15.0 29 24.0 30 27.0 31 51.0 32 65.0 33 123.0 34 242.0 35 679.0 36 2564.0 37 158.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 36.97942799799298 21.876567987957852 12.56899147014551 28.57501254390366 2 27.224999999999998 26.125 31.25 15.4 3 21.45 28.125 31.374999999999996 19.05 4 24.65 35.5 21.75 18.099999999999998 5 26.525 36.4 19.275000000000002 17.8 6 19.05 40.300000000000004 22.025 18.625 7 20.0 22.375 39.050000000000004 18.575 8 21.05 24.2 29.425 25.324999999999996 9 20.674999999999997 24.825 29.975 24.525 10-14 22.29 29.794999999999998 26.63 21.285 15-19 22.040000000000003 27.689999999999998 28.53 21.740000000000002 20-24 23.190797699424856 27.786946736684172 27.541885471367845 21.48037009252313 25-29 22.826413206603302 28.81940970485243 27.793896948474238 20.560280140070038 30-34 22.83799329765418 28.569999499824938 27.799729905466915 20.792277297053968 35-39 22.956887066119837 27.47324197259178 28.183455036510953 21.38641592477743 40-44 22.87300555194318 28.114840194067924 27.279547841744613 21.732606412244284 45-49 23.036911073321996 28.04341302390717 28.573572071621488 20.346103831149346 50-54 22.55563890972743 27.76194048512128 28.017004251062765 21.66541635408852 55-59 23.676838419209606 27.738869434717362 27.70385192596298 20.880440220110057 60-64 22.7518255476643 27.553265979793938 27.463238971691506 22.231669500850256 65-69 22.922292229222922 27.117711771177117 27.817781778177817 22.142214221422144 70-74 22.236118059029515 27.41870935467734 28.429214607303656 21.915957978989496 75-79 22.644057623049218 27.62104841936775 27.521008403361346 22.213885554221687 80-84 24.246061515378845 27.361840460115026 27.301825456364092 21.090272568142034 85-89 23.610624781151518 26.98214196388375 28.31274073333 21.094492521634738 90-94 23.67210163048915 27.728318495548663 26.788036410923276 21.811543463038912 95-99 23.10077519379845 27.161790447611907 28.18704676169042 21.550387596899228 100-104 23.676838419209606 28.009004502251127 27.028514257128567 21.285642821410704 105-109 23.57060677304787 27.39732879795908 26.98214196388375 22.049922465109297 110-114 24.03220966289887 28.258477543262977 27.928378513554065 19.780934280284086 115-119 24.054621848739497 28.38635454181673 27.100840336134453 20.458183273309324 120-124 23.681840920460232 27.723861930965484 27.878939469734863 20.715357678839418 125-129 24.08463385354142 28.096238495398158 27.235894357743096 20.583233293317328 130-134 25.027513756878438 28.644322161080538 25.807903951975987 20.520260130065033 135-139 25.812906453226613 27.348674337168582 27.028514257128567 19.809904952476238 140-144 26.172851855556665 27.35820746223867 26.302890867260174 20.166049814944483 145-149 26.178089044522263 27.263631815907953 26.728364182091045 19.82991495747874 150-151 26.150575287643825 27.388694347173587 27.301150575287643 19.15957978989495 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 0.0 10 0.5 11 0.5 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.5 18 1.5 19 2.0 20 1.5 21 0.5 22 1.0 23 1.5 24 2.0 25 4.0 26 3.0 27 3.0 28 7.5 29 16.0 30 23.5 31 21.5 32 20.5 33 29.5 34 49.0 35 65.5 36 74.5 37 92.0 38 122.5 39 164.0 40 185.0 41 197.0 42 239.5 43 267.5 44 282.5 45 295.0 46 292.0 47 263.5 48 224.0 49 196.5 50 179.0 51 148.5 52 111.0 53 89.5 54 70.5 55 62.5 56 50.5 57 33.5 58 18.0 59 15.5 60 17.5 61 14.5 62 8.0 63 4.0 64 6.0 65 5.0 66 3.5 67 1.0 68 0.5 69 1.0 70 0.5 71 0.0 72 0.0 73 0.5 74 0.5 75 0.0 76 0.5 77 1.5 78 1.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35000000000000003 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.025 25-29 0.05 30-34 0.034999999999999996 35-39 0.03 40-44 0.034999999999999996 45-49 0.03 50-54 0.025 55-59 0.05 60-64 0.03 65-69 0.01 70-74 0.05 75-79 0.04 80-84 0.025 85-89 0.045 90-94 0.03 95-99 0.025 100-104 0.05 105-109 0.045 110-114 0.03 115-119 0.04 120-124 0.05 125-129 0.04 130-134 0.05 135-139 0.05 140-144 0.03 145-149 0.05 150-151 0.05 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.0 #Duplication Level Percentage of deduplicated Percentage of total 1 68.48484848484848 45.2 2 19.242424242424242 25.4 3 7.878787878787878 15.6 4 2.5757575757575757 6.800000000000001 5 0.946969696969697 3.125 6 0.7196969696969697 2.85 7 0.0 0.0 8 0.03787878787878788 0.2 9 0.03787878787878788 0.22499999999999998 >10 0.07575757575757576 0.6 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CAGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 14 0.35000000000000003 No Hit CATTACCATCAATGTCTTCAAGCTATACTATGGTGATGACTGGGAAGGGC 10 0.25 No Hit AGGAGGTTCTGGATATATTGGTTCCTGGCTCGTCATGAAGCTGCTGGGGA 9 0.22499999999999998 No Hit ATAGAATGTCTTCTTATTGTTACTGCAATGGATGAAACCAGATCACTACC 8 0.2 No Hit CAAAAGACTGCTAAAACAGATGAAAGTTCCAGGAACGAGTTATTTAGTGG 6 0.15 No Hit TATCAATGTAAACAACGCTTTGAACTACATAGAGGAAGGAGCCAGCCATG 6 0.15 No Hit AAGAAGATCAAGACCGATACGCCTTATGGAACTGGTGGTGGCATGAACCT 6 0.15 No Hit TCGGATTTTACAAGGGAGTGCAGAGTGCAGGAGCCGCAGTTGCCTGGCAA 6 0.15 No Hit GGAGAGGCGAAGAAATGGGAAAGGACTACAACGAGGCCATTGAATCTCTC 6 0.15 No Hit GCAGCTTCGGGCAATCCACACTCCAAATTCCAAAATAAAGCAAAATCACC 6 0.15 No Hit ACAGCAATAGCTAGTCCGCATCATCAGCTAAACAATGGCAGCAGCAACAA 6 0.15 No Hit GCAAAATTCACCCTTGCTTGCTGCTTTACAGGCTCACTATATGTTGGGAC 6 0.15 No Hit CACCAATTAATGGAGTGCTTAAGCAGGGGCCTTTTTTTGAACCTTCTGTG 6 0.15 No Hit GTGAAATCGATCCAAATGGCCGAAATTGCGTTTTTAGGCGGTGGTGGTGT 6 0.15 No Hit AAACCACATATAGAGGGTGTAATAGCTAAGTAGCCTGTAAGAGATGGCTT 6 0.15 No Hit GGAAGAGCTGAAAACTGTAGCAGCTGCGAAAGTGGAGCAGATAACAGCTG 6 0.15 No Hit CTGAGAGTGAAAGCAAGGCAGAGTTAGGCAGGTTCTGTGATGCGCTTATC 6 0.15 No Hit GAAGAAATCAGACCTTCACCAGTGCAAGCACTTGGATGTGGTGTGGGTTT 6 0.15 No Hit GTTCTTGATCTCGAATCTTCTTTTCACTTTAATGTCTCTAAAACTAAAGG 6 0.15 No Hit AGGGCTGACTACAAAATCAGGAAAATTGAGAGAAATGACCCCAATACTCA 6 0.15 No Hit GTATCAATGGACCTCTCATAGAAGCTATTTCCGTCACTCGTGCTTTTTAT 6 0.15 No Hit GTCAAAGTAAAAGTCCACAGTGGCATGGAGTCAAGTAGGGATGCCGGTAT 6 0.15 No Hit GAGGCAGTTAAAGTAGGAGGCACAAATCACAGTCATGCTACTCAGGATCT 6 0.15 No Hit GAATGTATGGCTTTGGACGAGTCTCTTGGCTGGAGGACAGCCACTCCCTG 5 0.125 No Hit AGAGGGTGTAATAGCTAAGTAGCCTGTAAGAGATGGCTTCCTCTGTGATT 5 0.125 No Hit ACCTCTTGAATGAGGCAGCTATCCTAGCTGGGCGGCGTGGAAAGACAGCA 5 0.125 No Hit TGGGACAGCAGCCTACCTTAATGTCTTCTCCTCCATCTTCTCGGTTCTGA 5 0.125 No Hit GAACTTCCCGGATCTAGCAGGAGCGGTTAATAAGCTGAGCGAGAGCGTCA 5 0.125 No Hit CAAGGGACAAAGCTAGAATCTCAATCACGTGTTCCACTGAAACCTCCTCA 5 0.125 No Hit TGAAGACGAATTGAAGCATGCAGTTGCATTTGTTCGTCCAGTTAGCGTTG 5 0.125 No Hit GGCGCAGGGAAGCACATCACAGCAGGGGCCAAGAAGGTGCTCATCACAGC 5 0.125 No Hit GCTATAGATGACATGTTATTCCGCTGTTTTGTATTAATCTGTTTATGTTT 5 0.125 No Hit TGCACATACAACAAAGGGAGTCTTTGTAAACCAGCTCTTGCCAATGCTGC 5 0.125 No Hit GAGAGACTGAGAGTGTTTCAGAATTCAGGAATGGCTTCCACTTCTTCTCT 5 0.125 No Hit GGTCACTCCTGGCGCTGAATGCAAGGACAGGGCCTCCCCTGACCAAGTTG 5 0.125 No Hit GCCGTCCAAGAAAAGTTTACTTGGTGGATTTTTCATGTTACAAGCCCGGA 5 0.125 No Hit GTTAGAACCTTTCAGGACTTATTGAAGGATGAGTATGATGAAGATTTGTT 5 0.125 No Hit GAACAACTTCGTATTTAGTTCATCCATTTGCTTCATCAATCAATCACCAT 5 0.125 No Hit GTTCTTCGATGGGATGCCAAAATGGCCACCTTCCAAGTATCATCTGACCT 5 0.125 No Hit GGAGAAAATACCATCACATAAGAAAACTGAAGAGGTCCCTCCCCATCCAG 5 0.125 No Hit CACATATATTTCCAAGATGAAGGCCTTTCTTATCGCATGCATTCTCTTGG 5 0.125 No Hit CCTGTACTCTCTAGTATTAATCCTAATTCCCATATTATCATAAATAGGTT 5 0.125 No Hit GGGTAAATCCAAGAGGCGTAGCTTATTCGATGATGAGGATGACCGATACT 5 0.125 No Hit AAAAAGATTATGAAAAAGATTATTGTGAACATAAAGGCAAAGGATGTTGG 5 0.125 No Hit CCCAGTCTCCACCTCTGGCCTCCGCACAACCAGCCTCCGTGGTCTTGGTT 5 0.125 No Hit AGAACACCGAGGCTAACCGCCAGGCATACCGTACCCTTCTTGTGACAGTC 5 0.125 No Hit CTTATGGCCACCGCTGCTCTCCTCCGCTCTCTACGACGCCGTGACGTCGC 5 0.125 No Hit AGCTGATATAGGTTGGGATTCGAAGGCGTGGGAGGATCCCATGGCATTCA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.025 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.1625 0.0 0.0 0.0 0.0 76-77 0.2375 0.0 0.0 0.0 0.0 78-79 0.275 0.0 0.0 0.0 0.0 80-81 0.375 0.0 0.0 0.0 0.0 82-83 0.375 0.0 0.0 0.0 0.0 84-85 0.4375 0.0 0.0 0.0 0.0 86-87 0.5875 0.0 0.0 0.0 0.0 88-89 0.7625 0.0 0.0 0.0 0.0 90-91 0.8375 0.0 0.0 0.0 0.0 92-93 0.9875 0.0 0.0 0.0 0.0 94-95 1.1625 0.0 0.0 0.0 0.0 96-97 1.25 0.0 0.0 0.0 0.0 98-99 1.4 0.0 0.0 0.0 0.0 100-101 1.475 0.0 0.0 0.0 0.0 102-103 1.6125 0.0 0.0 0.0 0.0 104-105 1.825 0.0 0.0 0.0 0.0 106-107 2.0125 0.0 0.0 0.0 0.0 108-109 2.1875 0.0 0.0 0.0 0.0 110-111 2.3625 0.0 0.0 0.0 0.0 112-113 2.6 0.0 0.0 0.0 0.0 114-115 2.8 0.0 0.0 0.0 0.0 116-117 3.15 0.0 0.0 0.0 0.0 118-119 3.5125 0.0 0.0 0.0 0.0 120-121 3.775 0.0 0.0 0.0 0.0 122-123 4.199999999999999 0.0 0.0 0.0 0.0 124-125 4.5875 0.0 0.0 0.0 0.0 126-127 4.875 0.0 0.0 0.0 0.0 128-129 5.35 0.0 0.0 0.0 0.0 130-131 6.0875 0.0 0.0 0.0 0.0 132-133 6.9 0.0 0.0 0.0 0.0 134-135 7.5625 0.0 0.0 0.0 0.0 136-137 8.15 0.0 0.0 0.0 0.0 138-139 8.8375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTACT 10 0.006830828 145.0 7 GTTCTGG 10 0.006830828 145.0 6 CACTGCA 10 0.006830828 145.0 6 ATTGTAC 10 0.006830828 145.0 6 TCTCTCT 30 0.0014437955 24.166668 120-124 >>END_MODULE Read 882343 spots for SRR13695460.sra Written 882343 spots for SRR13695460.sra Read 882343 spots for SRR13695460.sra Written 882343 spots for SRR13695460.sra Read 882343 spots for SRR13695460.sra Written 882343 spots for SRR13695460.sra Read 882343 spots for SRR13695460.sra Written 882343 spots for SRR13695460.sra Read 882343 spots for SRR13695460.sra Written 882343 spots for SRR13695460.sra Read 882343 spots for SRR13695460.sra Written 882343 spots for SRR13695460.sra Read 882343 spots for SRR13695460.sra Written 882343 spots for SRR13695460.sra Read 882343 spots for SRR13695460.sra Written 882343 spots for SRR13695460.sra Read 882343 spots for SRR13695460.sra Written 882343 spots for SRR13695460.sra Read 882343 spots for SRR13695460.sra Written 882343 spots for SRR13695460.sra Read 882343 spots for SRR13695460.sra Written 882343 spots for SRR13695460.sra Read 882343 spots for SRR13695460.sra Written 882343 spots for SRR13695460.sra Read 882343 spots for SRR13695460.sra Written 882343 spots for SRR13695460.sra Read 882343 spots for SRR13695460.sra Written 882343 spots for SRR13695460.sra Read 882343 spots for SRR13695460.sra Written 882343 spots for SRR13695460.sra Read 882343 spots for SRR13695460.sra Written 882343 spots for SRR13695460.sra Read 882343 spots for SRR13695460.sra Written 882343 spots for SRR13695460.sra Read 882343 spots for SRR13695460.sra Written 882343 spots for SRR13695460.sra Read 882343 spots for SRR13695460.sra Written 882343 spots for SRR13695460.sra Read 882359 spots for SRR13695460.sra Written 882359 spots for SRR13695460.sra SRR ids: ['SRR13695460.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_z3064nz8 SRR13695460.sra spots: 17646876 blocks: [[1, 882343], [882344, 1764686], [1764687, 2647029], [2647030, 3529372], [3529373, 4411715], [4411716, 5294058], [5294059, 6176401], [6176402, 7058744], [7058745, 7941087], [7941088, 8823430], [8823431, 9705773], [9705774, 10588116], [10588117, 11470459], [11470460, 12352802], [12352803, 13235145], [13235146, 14117488], [14117489, 14999831], [14999832, 15882174], [15882175, 16764517], [16764518, 17646876]] SRR13695460 file size 5975480 SRR13695460 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695460 SRR13695460_1.fastq SRR13695460_2.fastq Input file: SRR13695460_1.fastq Paired file: SRR13695460_2.fastq trimmed: SRR13695460-trimmed-pair1.fastq, SRR13695460-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 04:48:59 2025 >> started Wed Feb 12 04:49:18 2025 >> done (18.904s) 17646876 read pairs processed; of these: 128 ( 0.00%) short read pairs filtered out after trimming by size control 1854 ( 0.01%) empty read pairs filtered out after trimming by size control 17644894 (99.99%) read pairs available; of these: 2026301 (11.48%) trimmed read pairs available after processing 15618593 (88.52%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 4 0.00% 19 0 0.00% 20 4 0.00% 21 0 0.00% 22 2 0.00% 23 1 0.00% 24 1 0.00% 25 1 0.00% 26 1 0.00% 27 3 0.00% 28 2 0.00% 29 2 0.00% 30 4 0.00% 31 1 0.00% 32 8 0.00% 33 3 0.00% 34 4 0.00% 35 3 0.00% 36 2 0.00% 37 9 0.00% 38 6 0.00% 39 9 0.00% 40 10 0.00% 41 12 0.00% 42 20 0.00% 43 31 0.00% 44 19 0.00% 45 10 0.00% 46 33 0.00% 47 27 0.00% 48 34 0.00% 49 59 0.00% 50 66 0.00% 51 92 0.00% 52 94 0.00% 53 83 0.00% 54 81 0.00% 55 109 0.00% 56 148 0.00% 57 166 0.00% 58 203 0.00% 59 226 0.00% 60 301 0.00% 61 345 0.00% 62 318 0.00% 63 393 0.00% 64 487 0.00% 65 507 0.00% 66 599 0.00% 67 610 0.00% 68 725 0.00% 69 928 0.01% 70 1023 0.01% 71 1202 0.01% 72 1308 0.01% 73 1602 0.01% 74 1791 0.01% 75 2005 0.01% 76 2073 0.01% 77 2452 0.01% 78 2633 0.01% 79 3052 0.02% 80 3092 0.02% 81 3785 0.02% 82 4096 0.02% 83 4714 0.03% 84 5079 0.03% 85 5812 0.03% 86 5949 0.03% 87 6373 0.04% 88 7069 0.04% 89 7655 0.04% 90 8204 0.05% 91 8843 0.05% 92 9371 0.05% 93 10084 0.06% 94 10809 0.06% 95 11560 0.07% 96 12210 0.07% 97 13027 0.07% 98 13649 0.08% 99 14105 0.08% 100 15184 0.09% 101 15526 0.09% 102 16367 0.09% 103 17417 0.10% 104 18003 0.10% 105 18619 0.11% 106 19570 0.11% 107 20351 0.12% 108 20995 0.12% 109 22083 0.13% 110 22450 0.13% 111 23356 0.13% 112 24345 0.14% 113 24764 0.14% 114 25955 0.15% 115 27123 0.15% 116 28366 0.16% 117 28948 0.16% 118 30000 0.17% 119 30509 0.17% 120 31630 0.18% 121 32380 0.18% 122 32937 0.19% 123 34307 0.19% 124 35307 0.20% 125 35335 0.20% 126 37131 0.21% 127 38091 0.22% 128 38819 0.22% 129 39664 0.22% 130 40845 0.23% 131 41235 0.23% 132 42104 0.24% 133 42779 0.24% 134 43700 0.25% 135 44376 0.25% 136 45991 0.26% 137 45900 0.26% 138 47491 0.27% 139 48998 0.28% 140 49213 0.28% 141 49910 0.28% 142 50575 0.29% 143 51281 0.29% 144 52324 0.30% 145 53224 0.30% 146 53522 0.30% 147 55453 0.31% 148 56018 0.32% 149 56873 0.32% 150 57524 0.33% 151 15618593 88.52% 17644894 reads passed initial QC criterion=sequence-density sequence-density=0.42 sequence-density-rank=1 fanout-score=2.01 fanout-score-rank=21 prefix-density=0.42 prefix-fanout=2.0 sequence=CGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTT criterion=fanout-score sequence-density=0.01 sequence-density-rank=25 fanout-score=435.31 fanout-score-rank=1 prefix-density=0.29 prefix-fanout=16.8 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGAGTATGAATGTGTTCTCTGT criterion=sequence-density sequence-density=0.64 sequence-density-rank=1 fanout-score=2.05 fanout-score-rank=27 prefix-density=0.65 prefix-fanout=2.0 sequence=AACCGCACCCCGGCACAGGCCAACATGGTTGCACCATTCAACGGCCTCAAGTCTACCTCAGCTTTCCCGGTCACCAGAAAGGC criterion=fanout-score sequence-density=0.01 sequence-density-rank=35 fanout-score=37.06 fanout-score-rank=1 prefix-density=0.17 prefix-fanout=1.8 sequence=GGAACTCAAACGCATAGCTTCCTACAAATACCCCAGCTAGCCAATACTCTCCACCACTAAACCCAAAGCTCAAAAAAAATCTTAACTGCACCCGCTCATCCAGCAATGGCAGCAGCAACAATGGCCCTCTC SRR13695460 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 04:49:59 Started mapping on | Feb 12 04:49:59 Finished on | Feb 12 04:51:46 Mapping speed, Million of reads per hour | 593.66 Number of input reads | 17644894 Average input read length | 296 UNIQUE READS: Uniquely mapped reads number | 16562533 Uniquely mapped reads % | 93.87% Average mapped length | 294.78 Number of splices: Total | 16311758 Number of splices: Annotated (sjdb) | 15965210 Number of splices: GT/AG | 15972777 Number of splices: GC/AG | 275817 Number of splices: AT/AC | 9158 Number of splices: Non-canonical | 54006 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 2.85 Insertion rate per base | 0.02% Insertion average length | 2.39 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 409089 % of reads mapped to multiple loci | 2.32% Number of reads mapped to too many loci | 91125 % of reads mapped to too many loci | 0.52% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.17% % of reads unmapped: other | 0.13% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 673483 673483 673483 N_multimapping 409089 409089 409089 N_noFeature 633800 16155436 884220 N_ambiguous 263768 1852 105848 UnstrandedReadsAssigned:15664965 PositiveStrandReadsAssigned:405245 NegativeStrandReadsAssigned:15572465 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695460 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695460-trimmed-pair1.fastq SRR13695460-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 17,644,894 reads, 15,611,363 reads pseudoaligned [quant] estimated average fragment length: 249.035 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,098 rounds 52401 SRR13695460.ke.tsv 34699 SRR13695460.se.tsv 87100 total ==> SRR13695460.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1769.96 369 11.9741 Potri.005G024800.1.v4.1 1035 786.965 305 22.2601 Potri.004G059700.1.v4.1 961 712.999 0 0 Potri.007G009000.2.v4.1 1416 1167.96 0 0 Potri.003G141000.2.v4.1 2943 2694.96 831 17.7105 Potri.016G087400.1.v4.1 270 84.2316 714 486.862 Potri.015G069301.1.v4.1 564 324.399 0 0 Potri.010G195200.1.v4.1 1773 1524.96 97 3.65338 Potri.012G127500.1.v4.1 977 728.98 74 5.83041 ==> SRR13695460.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 102 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 202 Potri.001G212900.v4.1 7 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 5 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 4 SRR13695460 completed mapping pipeline successfully