Starting /dee2/code/volunteer_pipeline.sh SRR13695461 current disk space = 3049109676032 free memory = 901674792 SRR13695461 SRAfilesize 3e7844e13a2c9b3f808ab03395e1b29e SRR13695461.sra SRR13695461.sra file validated SRR13695461 is paired end SRR13695461 is conventional basespace SRR13695461 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695461_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5165 37.0 37.0 37.0 37.0 37.0 2 36.32425 37.0 37.0 37.0 37.0 37.0 3 36.4715 37.0 37.0 37.0 37.0 37.0 4 36.535 37.0 37.0 37.0 37.0 37.0 5 36.5975 37.0 37.0 37.0 37.0 37.0 6 36.569 37.0 37.0 37.0 37.0 37.0 7 36.474 37.0 37.0 37.0 37.0 37.0 8 36.584 37.0 37.0 37.0 37.0 37.0 9 36.554 37.0 37.0 37.0 37.0 37.0 10-14 36.5662 37.0 37.0 37.0 37.0 37.0 15-19 36.505700000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.5256 37.0 37.0 37.0 37.0 37.0 25-29 36.418600000000005 37.0 37.0 37.0 37.0 37.0 30-34 36.3806 37.0 37.0 37.0 37.0 37.0 35-39 36.4222 37.0 37.0 37.0 37.0 37.0 40-44 36.4337 37.0 37.0 37.0 37.0 37.0 45-49 36.428900000000006 37.0 37.0 37.0 37.0 37.0 50-54 36.3487 37.0 37.0 37.0 37.0 37.0 55-59 36.3623 37.0 37.0 37.0 37.0 37.0 60-64 36.339800000000004 37.0 37.0 37.0 37.0 37.0 65-69 36.2843 37.0 37.0 37.0 37.0 37.0 70-74 36.2804 37.0 37.0 37.0 37.0 37.0 75-79 36.288599999999995 37.0 37.0 37.0 37.0 37.0 80-84 36.2115 37.0 37.0 37.0 37.0 37.0 85-89 36.2402 37.0 37.0 37.0 37.0 37.0 90-94 36.191 37.0 37.0 37.0 37.0 37.0 95-99 36.1364 37.0 37.0 37.0 37.0 37.0 100-104 36.1492 37.0 37.0 37.0 37.0 37.0 105-109 36.0882 37.0 37.0 37.0 37.0 37.0 110-114 36.027100000000004 37.0 37.0 37.0 37.0 37.0 115-119 36.0589 37.0 37.0 37.0 37.0 37.0 120-124 36.028600000000004 37.0 37.0 37.0 37.0 37.0 125-129 35.9803 37.0 37.0 37.0 37.0 37.0 130-134 35.918800000000005 37.0 37.0 37.0 37.0 37.0 135-139 35.9289 37.0 37.0 37.0 37.0 37.0 140-144 35.8341 37.0 37.0 37.0 37.0 37.0 145-149 35.664 37.0 37.0 37.0 37.0 37.0 150-151 35.432500000000005 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 1.0 23 2.0 24 1.0 25 2.0 26 3.0 27 10.0 28 11.0 29 15.0 30 22.0 31 46.0 32 58.0 33 62.0 34 140.0 35 323.0 36 2938.0 37 366.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.275 12.575 8.6 36.55 2 21.122525682786268 12.15234277123528 34.70308193435229 32.02204961162616 3 18.224999999999998 17.325 28.299999999999997 36.15 4 22.5 26.525 23.275000000000002 27.700000000000003 5 22.675 31.3 23.425 22.6 6 21.825 35.225 22.7 20.25 7 16.25 27.700000000000003 39.5 16.55 8 16.900000000000002 27.075 32.625 23.400000000000002 9 17.299999999999997 24.0 34.449999999999996 24.25 10-14 20.11 29.854999999999997 27.29 22.745 15-19 19.98 27.97 27.939999999999998 24.11 20-24 19.919999999999998 28.439999999999998 27.47 24.169999999999998 25-29 19.580000000000002 28.705000000000002 28.22 23.494999999999997 30-34 20.28 28.9 27.755000000000003 23.064999999999998 35-39 19.805 28.73 27.060000000000002 24.404999999999998 40-44 20.44 28.055000000000003 27.750000000000004 23.755000000000003 45-49 20.405 28.694999999999997 27.58 23.32 50-54 20.119999999999997 27.965 28.405 23.51 55-59 20.935000000000002 28.525 27.365000000000002 23.175 60-64 20.485 28.395 27.3 23.82 65-69 20.04 28.42 27.735 23.805 70-74 20.505000000000003 28.975 27.365000000000002 23.155 75-79 20.49 28.444999999999997 27.43 23.635 80-84 19.939999999999998 28.38 27.905 23.775 85-89 21.310000000000002 28.355000000000004 26.865 23.47 90-94 21.295 28.52 26.755000000000003 23.43 95-99 21.13 27.63 27.915 23.325000000000003 100-104 21.279999999999998 28.95 26.765 23.005 105-109 20.745 28.249999999999996 26.995 24.01 110-114 20.880000000000003 28.225 27.095000000000002 23.799999999999997 115-119 20.330000000000002 28.4 27.639999999999997 23.630000000000003 120-124 21.055 28.275 27.365000000000002 23.305 125-129 21.175 28.144999999999996 27.689999999999998 22.99 130-134 20.495 28.144999999999996 27.505000000000003 23.855 135-139 21.435000000000002 28.439999999999998 25.679999999999996 24.445 140-144 20.665 29.270000000000003 27.205000000000002 22.86 145-149 20.855 28.51 27.26 23.375 150-151 22.25 27.962500000000002 27.125 22.662499999999998 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.0 19 0.0 20 0.5 21 0.5 22 0.0 23 1.5 24 3.5 25 2.5 26 0.5 27 2.0 28 3.0 29 5.0 30 16.0 31 21.0 32 25.5 33 42.0 34 58.5 35 69.5 36 94.5 37 120.0 38 148.5 39 161.5 40 176.5 41 208.5 42 222.0 43 228.5 44 245.5 45 269.0 46 276.0 47 264.5 48 229.5 49 207.0 50 183.5 51 146.0 52 141.5 53 120.5 54 72.0 55 61.5 56 49.0 57 30.5 58 22.0 59 18.5 60 19.5 61 9.5 62 5.5 63 5.0 64 2.5 65 2.5 66 2.5 67 1.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.5 73 1.0 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.22499999999999998 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.7 #Duplication Level Percentage of deduplicated Percentage of total 1 69.79320531757755 47.25 2 18.94387001477105 25.650000000000002 3 7.348596750369277 14.924999999999999 4 2.363367799113737 6.4 5 1.1078286558345642 3.75 6 0.1846381093057607 0.75 7 0.1846381093057607 0.8750000000000001 8 0.07385524372230429 0.4 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTGACAACGGCAGCACCAACGTCTTTCATTGAGGCCTTGATGCTGAGCCT 8 0.2 No Hit CCAGTTAGTAACCACACCAGAAACTCTGCCTCCTTTCACTATTAAATCCT 8 0.2 No Hit GGACCTTACGAGAAGGAGGCCGTCGGAATAAACTTATAAGATCGTCAAGA 7 0.17500000000000002 No Hit CCTGATTTTATTACCACTCCATCACATATTATTGCATGCCTTATGTCACA 7 0.17500000000000002 No Hit GCTTTGTAGATACATTCCTCTCGCCGAAAGGTAGTCTCCAAGTCCTCTGG 7 0.17500000000000002 No Hit GGAAAAGAGAACCTGACAACAACAACAAAGCTGAAAACTCAACCATGGCT 7 0.17500000000000002 No Hit GCCCCAGCTGCTTCAGCTACCTCTGGAATCACCATGGAAGCTGTAAGTGC 7 0.17500000000000002 No Hit GCCACCTTGGACCACGTATGGTCTCTTAACCCCAAGCTTTCTCAAGGATC 6 0.15 No Hit ATGGGGGTGCAGCACTGCTAGATGGTGGGGTTGAGACACTTGTTAGTGCC 6 0.15 No Hit GTTTGTTCATGTTGTACCGCATGCTTCGTAAATTCAGCTGATAAGATTTG 6 0.15 No Hit CAGGAAACCGGTCTCCCATTAGCTCATAAGGCTTGGCAGGGAACACATAC 6 0.15 No Hit CTTGCATCCATGCACATAACCGTTAACTGCTTATGGATTAAATAAGCAAA 6 0.15 No Hit GCACCGGACAATGGAGGCAGAAGGCAAGCGGAGGGTCTGACCCTTAACAA 5 0.125 No Hit CTATCCCCATCACGATGAAATTTCAAAGATTACCCGGGCCTGTCGGCCAA 5 0.125 No Hit ATCTCGTATAGCTGCAGTGGGATTTTCGCAAGTTCCCAACATCCTTCCAT 5 0.125 No Hit GTGAAATCTTTGCTTTGTCTTCGACCAGAGAAGAAACAGCGTGGATGAAT 5 0.125 No Hit GCCATGATTACAATCTCCATCCGGGCTTTGATACCATAAAAGAGTTTGAC 5 0.125 No Hit GCTGGAACTTAAAGGACTGAAGATCATATGAAGGTTGTGTTAAGGACATG 5 0.125 No Hit GCTCACTCGAGACCGGTTTTTCACCAGACTACTGGCCACTGGAGATGCAA 5 0.125 No Hit ATTTCGTTCCTGACCATTCATTTGATCACAAGCAGGTGGAACTGCAAATC 5 0.125 No Hit CTCCAAAGCAAATGATTCAATGGCTGAGGCAGTTCTAGCACCCTCATATG 5 0.125 No Hit ATCTGGTAATTAATGTATAGGATACAAACGGCACACGAATGCTCAAAGAT 5 0.125 No Hit ACTCGACTTCCCTGCTTCCCTCATGTACCGAACATCTCCGTTCATCAGCT 5 0.125 No Hit CTCGAAAGAAGAGGGAAGAAATGAGGCAATTTCTTCTCAAACGAAGAGTC 5 0.125 No Hit GCTGTGCATGATGGAGACGTTGACAGATATCATTCCATTTATTTCGCAGC 5 0.125 No Hit CTCAATTCTTCTTCTTTCTCTGTCAACTCTCGATACTGATACCACTGTGC 5 0.125 No Hit AGCCAAAGGAAGTCCCATCTTGAAGCTGTAGAAAAGAGGACGCTGAATGA 5 0.125 No Hit ACCGAGACATTTGGGGTTGGAACTCGGAGGGCAATGCCATTGAGTTTGCC 5 0.125 No Hit TGCACATTCGACAACACCAGCATCTCCCCTCAAGCCACGGAGGCAGGCAT 5 0.125 No Hit CTCATGACTTTTCCAAGATGGTCGACTCTTTTGACCATTTAACTTCTTCT 5 0.125 No Hit GGGATCTTACTAACATCTAACACCCAAAAATCTCCCTTCCTGTGTCTCTG 5 0.125 No Hit CTTGCTTTATACCCTTAATTAGCTTCTAATGATTCATGTTCCACGGAGAC 5 0.125 No Hit CGGAACTTTCACATATCGAGGCCATTCACGAATCACGTCATGCATAAGGT 5 0.125 No Hit GGGGTCTTTCTGTCCAGGTGCAGGTAGTCCGCATCTTCACAGACATGTCT 5 0.125 No Hit ACAAGGTAGAAAAAAGCCAAACGTTTTGCATTATTTTTACATTCCTTGTC 5 0.125 No Hit ACCCAGTGTAAGCTCTTCAAGGGTAGTTTCAAAAGCATGAGTATATCCGA 5 0.125 No Hit GTGTGCTGTTTAAACTCTCGTCTGTGACCTGCTGATAGTGGCCTTTGACA 5 0.125 No Hit CGGAACTGTATCCCTGGAATCAGGTATCCAGAATGGTTGCTACGTGTACA 5 0.125 No Hit GGCTGCCATTACTGGTATGCTGTGATAGAAGCAATCCTTGCGCAATTTCT 5 0.125 No Hit CGCTGAAGCTAATTAACTCAACTTCTACTCTATCTCCTGTCCTAAGCAAA 5 0.125 No Hit CGCTGTGGAGAGTTTGATTCTTTGCTAGACGGACAGTGTTAACATATGCC 5 0.125 No Hit CTCGTGAAATAGTTTTCTAGCATCCTCAGGTGTGACTGATGGCACAGCTC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0125 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.037500000000000006 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.0875 0.0 0.0 0.0 0.0 84-85 0.1125 0.0 0.0 0.0 0.0 86-87 0.15 0.0 0.0 0.0 0.0 88-89 0.1875 0.0 0.0 0.0 0.0 90-91 0.225 0.0 0.0 0.0 0.0 92-93 0.2625 0.0 0.0 0.0 0.0 94-95 0.2875 0.0 0.0 0.0 0.0 96-97 0.4 0.0 0.0 0.0 0.0 98-99 0.45 0.0 0.0 0.0 0.0 100-101 0.525 0.0 0.0 0.0 0.0 102-103 0.7125 0.0 0.0 0.0 0.0 104-105 1.025 0.0 0.0 0.0 0.0 106-107 1.2375 0.0 0.0 0.0 0.0 108-109 1.4125 0.0 0.0 0.0 0.0 110-111 1.6625 0.0 0.0 0.0 0.0 112-113 1.925 0.0 0.0 0.0 0.0 114-115 2.2125 0.0 0.0 0.0 0.0 116-117 2.4124999999999996 0.0 0.0 0.0 0.0 118-119 2.9 0.0 0.0 0.0 0.0 120-121 3.3499999999999996 0.0 0.0 0.0 0.0 122-123 3.6875 0.0 0.0 0.0 0.0 124-125 3.9125 0.0 0.0 0.0 0.0 126-127 4.2125 0.0 0.0 0.0 0.0 128-129 4.6375 0.0 0.0 0.0 0.0 130-131 5.074999999999999 0.0 0.0 0.0 0.0 132-133 5.637499999999999 0.0 0.0 0.0 0.0 134-135 6.112500000000001 0.0 0.0 0.0 0.0 136-137 6.4 0.0 0.0 0.0 0.0 138-139 6.85 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATCACC 10 0.006830828 145.0 3 ACCGAAG 10 0.006830828 145.0 7 AAAAGAC 10 0.006830828 145.0 2 AAGACCG 10 0.006830828 145.0 4 GACCGAA 10 0.006830828 145.0 6 AGACCGA 10 0.006830828 145.0 5 CCGAAGC 10 0.006830828 145.0 8 >>END_MODULE SRR13695461 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695461_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.05725 37.0 37.0 37.0 37.0 37.0 2 36.1145 37.0 37.0 37.0 37.0 37.0 3 36.086 37.0 37.0 37.0 37.0 37.0 4 36.228 37.0 37.0 37.0 37.0 37.0 5 36.2855 37.0 37.0 37.0 37.0 37.0 6 36.238 37.0 37.0 37.0 37.0 37.0 7 36.282 37.0 37.0 37.0 37.0 37.0 8 36.257 37.0 37.0 37.0 37.0 37.0 9 36.198 37.0 37.0 37.0 37.0 37.0 10-14 36.242399999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.205 37.0 37.0 37.0 37.0 37.0 20-24 36.1967 37.0 37.0 37.0 37.0 37.0 25-29 36.1383 37.0 37.0 37.0 37.0 37.0 30-34 36.10574999999999 37.0 37.0 37.0 37.0 37.0 35-39 36.047 37.0 37.0 37.0 37.0 37.0 40-44 36.1021 37.0 37.0 37.0 37.0 37.0 45-49 36.03865 37.0 37.0 37.0 37.0 37.0 50-54 35.998000000000005 37.0 37.0 37.0 37.0 37.0 55-59 36.0074 37.0 37.0 37.0 37.0 37.0 60-64 35.9551 37.0 37.0 37.0 37.0 37.0 65-69 35.87075 37.0 37.0 37.0 37.0 37.0 70-74 35.853300000000004 37.0 37.0 37.0 37.0 37.0 75-79 35.82090000000001 37.0 37.0 37.0 37.0 37.0 80-84 35.881150000000005 37.0 37.0 37.0 37.0 37.0 85-89 35.72685 37.0 37.0 37.0 37.0 37.0 90-94 35.6958 37.0 37.0 37.0 37.0 37.0 95-99 35.77825 37.0 37.0 37.0 37.0 37.0 100-104 35.7223 37.0 37.0 37.0 37.0 37.0 105-109 35.723699999999994 37.0 37.0 37.0 37.0 37.0 110-114 35.5885 37.0 37.0 37.0 37.0 37.0 115-119 35.57425 37.0 37.0 37.0 37.0 37.0 120-124 35.593900000000005 37.0 37.0 37.0 37.0 37.0 125-129 35.61905 37.0 37.0 37.0 37.0 37.0 130-134 35.504999999999995 37.0 37.0 37.0 37.0 37.0 135-139 35.574 37.0 37.0 37.0 37.0 37.0 140-144 35.4424 37.0 37.0 37.0 37.0 37.0 145-149 35.3095 37.0 37.0 37.0 29.8 37.0 150-151 34.99725 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 0.0 15 0.0 16 1.0 17 2.0 18 2.0 19 4.0 20 0.0 21 2.0 22 9.0 23 6.0 24 3.0 25 4.0 26 11.0 27 10.0 28 9.0 29 21.0 30 28.0 31 36.0 32 58.0 33 108.0 34 224.0 35 634.0 36 2654.0 37 172.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.45181476846058 21.201501877346686 12.816020025031289 24.53066332916145 2 29.425 24.85 28.525 17.2 3 20.875 27.500000000000004 32.625 19.0 4 23.95 32.074999999999996 23.95 20.025000000000002 5 25.75 36.05 21.875 16.325 6 18.275 39.25 23.175 19.3 7 19.7 23.325000000000003 38.425 18.55 8 19.05 29.349999999999998 27.975 23.625 9 20.825 25.124999999999996 29.775000000000002 24.275 10-14 22.78 30.29 25.6 21.33 15-19 22.400000000000002 29.07 27.450000000000003 21.08 20-24 23.046914074222265 29.388816644993497 27.128138441532464 20.436130839251774 25-29 22.406203101550776 29.21960980490245 27.188594297148573 21.1855927963982 30-34 22.592907517631172 28.690041514530083 27.3445705997099 21.372480368128848 35-39 21.996598979693907 28.368510553165947 28.203461038311495 21.43142942882865 40-44 23.184273709483794 27.460984393757503 27.906162464985997 21.44857943177271 45-49 22.507877757215024 28.229880458160356 27.419596858900615 21.842644925724002 50-54 23.632089626888067 27.938381514454335 27.273181954586377 21.15634690407122 55-59 23.451725862931465 27.613806903451728 28.33416708354177 20.60030015007504 60-64 23.091927578273484 28.463539061718517 27.09812943883165 21.346403921176353 65-69 22.82342351352703 28.159223883582534 27.714157123568533 21.3031954793219 70-74 23.301650825412707 27.863931965982992 27.023511755877937 21.810905452726363 75-79 22.54901960784314 27.43097238895558 27.96618647458984 22.053821528611444 80-84 23.305826456614152 28.367091772943237 27.081770442610654 21.24531132783196 85-89 23.995798109149117 27.43234455504977 28.122655194837677 20.449202140963433 90-94 23.687106131839553 28.428528558567574 27.513253976192857 20.37111133340002 95-99 24.166041510377596 27.161790447611907 27.206801700425103 21.465366341585394 100-104 23.761880940470235 28.009004502251127 27.313656828414207 20.915457728864432 105-109 23.43671835917959 27.378689344672335 27.863931965982992 21.320660330165083 110-114 23.372011603481045 28.71361408422527 27.263178953686108 20.651195358607584 115-119 23.520584262918312 28.242709219148615 26.98214196388375 21.25456455404932 120-124 24.327163581790895 28.25912956478239 26.973486743371687 20.440220110055026 125-129 24.310939922965336 28.757941073483067 26.431894352458606 20.49922465109299 130-134 24.252126063031515 27.058529264632313 27.848924462231118 20.84042021010505 135-139 24.627313656828413 28.31415707853927 26.463231615807903 20.595297648824413 140-144 24.562368710613182 28.013404021206362 27.57827348204461 19.84595378613584 145-149 25.202601300650322 27.938969484742373 27.10855427713857 19.749874937468732 150-151 25.22511255627814 28.70185092546273 26.675837918959477 19.39719859929965 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 1.0 5 1.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.5 16 0.5 17 0.0 18 0.0 19 0.0 20 1.5 21 2.0 22 1.0 23 2.0 24 2.5 25 1.5 26 5.5 27 7.5 28 6.5 29 12.5 30 14.5 31 19.5 32 33.0 33 32.5 34 42.5 35 73.5 36 81.0 37 105.0 38 130.5 39 172.5 40 221.0 41 218.5 42 225.5 43 252.0 44 262.0 45 278.5 46 262.0 47 234.5 48 233.5 49 205.5 50 165.5 51 139.0 52 114.0 53 90.0 54 83.0 55 66.0 56 56.0 57 44.0 58 26.0 59 18.0 60 11.5 61 8.5 62 6.0 63 3.0 64 3.0 65 4.5 66 4.5 67 2.5 68 1.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.5 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 1.0 80 1.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.125 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.03 25-29 0.05 30-34 0.034999999999999996 35-39 0.03 40-44 0.04 45-49 0.034999999999999996 50-54 0.03 55-59 0.05 60-64 0.03 65-69 0.015 70-74 0.05 75-79 0.04 80-84 0.025 85-89 0.045 90-94 0.03 95-99 0.025 100-104 0.05 105-109 0.05 110-114 0.03 115-119 0.045 120-124 0.05 125-129 0.045 130-134 0.05 135-139 0.05 140-144 0.03 145-149 0.05 150-151 0.05 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.625 #Duplication Level Percentage of deduplicated Percentage of total 1 71.29326047358833 48.925000000000004 2 17.88706739526412 24.55 3 7.067395264116576 14.549999999999999 4 2.2222222222222223 6.1 5 0.9836065573770493 3.375 6 0.29143897996357016 1.2 7 0.14571948998178508 0.7000000000000001 8 0.1092896174863388 0.6 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGGTCTTTCTTGTGCTTATGAGCTAAGCAAGAACCCTTCAGTTCGTGTTG 8 0.2 No Hit CAGTAAGGACATAATTCAAAGGTGGACTTACCCATTTGTACCAGATGTGA 8 0.2 No Hit GCACACAATACCAGCAAAAACCAGAACAAAAAACCTCTAGAATGGCCACT 8 0.2 No Hit AGGGTCATATTTGATTATACTTTTCACTTTCCTTGTCATCATAACAAGGG 7 0.17500000000000002 No Hit GTTGAGAAGAGAGTAGAGAGGGCAGGAGCGAAGATCACAGAAACAAATGG 7 0.17500000000000002 No Hit TGGCATTTAATCTTTCTACTATGGAAGAAACATGTGAGCCCTTGGCCTCC 7 0.17500000000000002 No Hit AAAAAGTTTGAGATGTCATGAAAGTCAGACTCCTGATAAATTTGATGTTT 7 0.17500000000000002 No Hit AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 6 0.15 No Hit CAAGATAGAGAGGAGATAAGATATAGACACTTGTTATAGGCTATCTTGCA 6 0.15 No Hit GAAGCTCTTGGCAGAGAAGGGAGACACCATACTCATTGATGTCCGGCCTG 6 0.15 No Hit GTTCTGTTTTCCACATTGTTATTACTCACAGCTTGGCCTGTCACCAATTT 6 0.15 No Hit CTACTCTTTAGCTCTCTCAAACCCCAATTCATTTCACACCCTATACTCAT 6 0.15 No Hit GCTTACTACAGAACTCCTCCTGTCCAATCCACTGACGATAGAGAAACAGG 6 0.15 No Hit CAATTGTATTTTTGACTTGGTGGCAAGGAGTGGCAATTGCTCTTCTCTAC 6 0.15 No Hit CATGAATATGAGCACCATGTCGACTACTAGAGCTGGAGTATTATGTTCAA 6 0.15 No Hit GAAACTTCTACCAGTGGACAATGCCTGTGGCTGATTCCTTTAGGTTCTGG 5 0.125 No Hit GGGAAAGATAAGACCAAACAAAAGGAAACGAATGATAAAAACGAGGATCA 5 0.125 No Hit AAAGCTGATAAGTTTTACCAGAAAGAACTTGGAGTTCTATTGACACCTCC 5 0.125 No Hit CTTCATCTTTTCTACCACAGATGCTATTATCACAAGCCATGTTCAGTGGT 5 0.125 No Hit GATGAAGAGCAATGAAAAGGGCATTCGAATAATCAACGATGCAGCTGAAG 5 0.125 No Hit CCTCTAGAGTAGAAGATTTGAATCTTGTAAATGCTGAAAATCTTGGAAAT 5 0.125 No Hit GGTCAACAACGTCAGTTGCTGATCAGAGCTCAGAGAAGTTACCTTCTTTG 5 0.125 No Hit GGTCAATCAAGTTGAACCCTTGAAGAGCTTACACTGGGGCACTAATCTGG 5 0.125 No Hit TATTGGTTCGGTACCCAGAATCAAATCAATACAAAACTCAATCTCTCTAT 5 0.125 No Hit TCCGCTTTGCTTTTGTGTCGTCACCGGCGGAGCCTGAGTTCCGCCGTCGT 5 0.125 No Hit GTACCATAATCGGCAAAACCTATACCCTCTCATTTGCAGTAGGAGATGCG 5 0.125 No Hit GCTCTATAATTTGCAAGACATACCTGAAGGAGTCTCCCTCCCGCGAGTTG 5 0.125 No Hit GACAAAAACCCTTTAACAAAACCTTATCAAGAACTCCTTGAATTACAGCC 5 0.125 No Hit GCTCTTGTACACAAGCCATCACTAGGAGTCTCATGCTCACCTGTTCTTGG 5 0.125 No Hit ACCACATATAGAGGGTGTAATAGCTAAGTAGCCTGTAAGAGATGGCTTCC 5 0.125 No Hit GTTAACTTTCTCCTCCACCTATCTCTGAATTCCTTTTCATGGTTCTCCAA 5 0.125 No Hit TGGCACTCAGGATGTTCGGTTTGAGGAAAAACAGTCTTACGAGGCTAGGA 5 0.125 No Hit AGGCCATGCTGGAGTTACAATTTTGCCCCTTCTGTCGCAGGCTAAGCCCC 5 0.125 No Hit CGGGGGCAGACCAAGAACAAGATATTACTCGGGGTACTCCAATTCTTATC 5 0.125 No Hit AGCTCCCAGAGTATAAACAGAAAGCAAAAGGCACCAGAGGACCGAAACTG 5 0.125 No Hit ATCTTGAACCGGTGGGCCCACTTCTCAAAAAAATATTCGTTTCTTCCACT 5 0.125 No Hit CAAAGCAAAAAGCTCCAGCTTCAACTCACTGGTAGTGATAATCTCTCACT 5 0.125 No Hit CGCAGGGAAGCACATCACAGCAGGGGCCAAGAAGGTGCTCATCACAGCCC 5 0.125 No Hit ATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAAT 5 0.125 No Hit GAAACTATGACTGGTGATGAGTTCCGAGCAATCCTCTCTGAATTTGTTGA 5 0.125 No Hit AGTGCCGGAAGGTCAAGGAAGTTGGTGACCTGATGACAGGGGAGCCGGCG 5 0.125 No Hit CCTTAGAAATTGTCAGTGGAAAGAGCAACTCAAGTTACAGGCCAGAGAAC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0125 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.037500000000000006 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.0875 0.0 0.0 0.0 0.0 84-85 0.1125 0.0 0.0 0.0 0.0 86-87 0.15 0.0 0.0 0.0 0.0 88-89 0.1875 0.0 0.0 0.0 0.0 90-91 0.225 0.0 0.0 0.0 0.0 92-93 0.2625 0.0 0.0 0.0 0.0 94-95 0.2875 0.0 0.0 0.0 0.0 96-97 0.4 0.0 0.0 0.0 0.0 98-99 0.45 0.0 0.0 0.0 0.0 100-101 0.525 0.0 0.0 0.0 0.0 102-103 0.7125 0.0 0.0 0.0 0.0 104-105 1.0499999999999998 0.0 0.0 0.0 0.0 106-107 1.275 0.0 0.0 0.0 0.0 108-109 1.475 0.0 0.0 0.0 0.0 110-111 1.7375 0.0 0.0 0.0 0.0 112-113 2.0 0.0 0.0 0.0 0.0 114-115 2.2875 0.0 0.0 0.0 0.0 116-117 2.4875 0.0 0.0 0.0 0.0 118-119 2.9625000000000004 0.0 0.0 0.0 0.0 120-121 3.375 0.0 0.0 0.0 0.0 122-123 3.7125 0.0 0.0 0.0 0.0 124-125 3.9375 0.0 0.0 0.0 0.0 126-127 4.2375 0.0 0.0 0.0 0.0 128-129 4.6625 0.0 0.0 0.0 0.0 130-131 5.1 0.0 0.0 0.0 0.0 132-133 5.6625 0.0 0.0 0.0 0.0 134-135 6.125 0.0 0.0 0.0 0.0 136-137 6.4 0.0 0.0 0.0 0.0 138-139 6.8375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTACTCC 10 0.006830828 145.0 8 GAAGCCT 10 0.006830828 145.0 145 CATTCTT 10 0.006830828 145.0 5 ACATTCT 10 0.006830828 145.0 4 AACTTAC 10 0.006830828 145.0 5 ACTTACT 10 0.006830828 145.0 6 CTTACTC 10 0.006830828 145.0 7 TACTCCC 10 0.006830828 145.0 9 TCTCAAC 10 0.006830828 145.0 1 GAGAGAG 85 0.002429728 34.11765 5 TTGTTCT 20 0.00593511 29.0 120-124 >>END_MODULE Read 764709 spots for SRR13695461.sra Written 764709 spots for SRR13695461.sra Read 764709 spots for SRR13695461.sra Written 764709 spots for SRR13695461.sra Read 764709 spots for SRR13695461.sra Written 764709 spots for SRR13695461.sra Read 764709 spots for SRR13695461.sra Written 764709 spots for SRR13695461.sra Read 764709 spots for SRR13695461.sra Written 764709 spots for SRR13695461.sra Read 764709 spots for SRR13695461.sra Written 764709 spots for SRR13695461.sra Read 764709 spots for SRR13695461.sra Written 764709 spots for SRR13695461.sra Read 764709 spots for SRR13695461.sra Written 764709 spots for SRR13695461.sra Read 764709 spots for SRR13695461.sra Written 764709 spots for SRR13695461.sra Read 764709 spots for SRR13695461.sra Written 764709 spots for SRR13695461.sra Read 764709 spots for SRR13695461.sra Written 764709 spots for SRR13695461.sra Read 764709 spots for SRR13695461.sra Written 764709 spots for SRR13695461.sra Read 764709 spots for SRR13695461.sra Written 764709 spots for SRR13695461.sra Read 764709 spots for SRR13695461.sra Written 764709 spots for SRR13695461.sra Read 764718 spots for SRR13695461.sra Written 764718 spots for SRR13695461.sra Read 764709 spots for SRR13695461.sra Written 764709 spots for SRR13695461.sra Read 764709 spots for SRR13695461.sra Written 764709 spots for SRR13695461.sra Read 764709 spots for SRR13695461.sra Written 764709 spots for SRR13695461.sra Read 764709 spots for SRR13695461.sra Written 764709 spots for SRR13695461.sra Read 764709 spots for SRR13695461.sra Written 764709 spots for SRR13695461.sra SRR ids: ['SRR13695461.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_g7yl9cjk SRR13695461.sra spots: 15294189 blocks: [[1, 764709], [764710, 1529418], [1529419, 2294127], [2294128, 3058836], [3058837, 3823545], [3823546, 4588254], [4588255, 5352963], [5352964, 6117672], [6117673, 6882381], [6882382, 7647090], [7647091, 8411799], [8411800, 9176508], [9176509, 9941217], [9941218, 10705926], [10705927, 11470635], [11470636, 12235344], [12235345, 13000053], [13000054, 13764762], [13764763, 14529471], [14529472, 15294189]] SRR13695461 file size 5175934 SRR13695461 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695461 SRR13695461_1.fastq SRR13695461_2.fastq Input file: SRR13695461_1.fastq Paired file: SRR13695461_2.fastq trimmed: SRR13695461-trimmed-pair1.fastq, SRR13695461-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 04:07:17 2025 >> started Wed Feb 12 04:07:34 2025 >> done (17.150s) 15294189 read pairs processed; of these: 89 ( 0.00%) short read pairs filtered out after trimming by size control 1802 ( 0.01%) empty read pairs filtered out after trimming by size control 15292298 (99.99%) read pairs available; of these: 1622518 (10.61%) trimmed read pairs available after processing 13669780 (89.39%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 0 0.00% 20 9 0.00% 21 0 0.00% 22 2 0.00% 23 10 0.00% 24 3 0.00% 25 3 0.00% 26 0 0.00% 27 1 0.00% 28 2 0.00% 29 3 0.00% 30 6 0.00% 31 6 0.00% 32 5 0.00% 33 3 0.00% 34 5 0.00% 35 3 0.00% 36 0 0.00% 37 3 0.00% 38 3 0.00% 39 5 0.00% 40 4 0.00% 41 19 0.00% 42 3 0.00% 43 7 0.00% 44 21 0.00% 45 11 0.00% 46 17 0.00% 47 16 0.00% 48 16 0.00% 49 26 0.00% 50 22 0.00% 51 26 0.00% 52 27 0.00% 53 55 0.00% 54 60 0.00% 55 83 0.00% 56 72 0.00% 57 70 0.00% 58 102 0.00% 59 116 0.00% 60 158 0.00% 61 147 0.00% 62 195 0.00% 63 242 0.00% 64 204 0.00% 65 290 0.00% 66 334 0.00% 67 387 0.00% 68 430 0.00% 69 519 0.00% 70 596 0.00% 71 709 0.00% 72 859 0.01% 73 952 0.01% 74 1036 0.01% 75 1254 0.01% 76 1391 0.01% 77 1554 0.01% 78 1839 0.01% 79 2009 0.01% 80 2229 0.01% 81 2588 0.02% 82 2942 0.02% 83 3030 0.02% 84 3688 0.02% 85 4074 0.03% 86 4233 0.03% 87 4580 0.03% 88 5091 0.03% 89 5243 0.03% 90 5938 0.04% 91 6176 0.04% 92 6709 0.04% 93 7408 0.05% 94 7899 0.05% 95 8545 0.06% 96 8866 0.06% 97 9504 0.06% 98 10174 0.07% 99 10579 0.07% 100 10945 0.07% 101 11478 0.08% 102 12502 0.08% 103 13145 0.09% 104 13909 0.09% 105 14538 0.10% 106 15353 0.10% 107 15767 0.10% 108 16104 0.11% 109 17365 0.11% 110 17667 0.12% 111 18209 0.12% 112 18974 0.12% 113 19433 0.13% 114 20547 0.13% 115 21212 0.14% 116 22131 0.14% 117 23075 0.15% 118 23928 0.16% 119 24584 0.16% 120 25187 0.16% 121 25930 0.17% 122 26553 0.17% 123 27317 0.18% 124 28678 0.19% 125 28963 0.19% 126 30264 0.20% 127 30711 0.20% 128 31738 0.21% 129 32604 0.21% 130 33343 0.22% 131 33369 0.22% 132 34354 0.22% 133 35067 0.23% 134 35782 0.23% 135 36589 0.24% 136 37509 0.25% 137 38213 0.25% 138 39229 0.26% 139 40640 0.27% 140 40877 0.27% 141 40868 0.27% 142 41845 0.27% 143 42066 0.28% 144 43283 0.28% 145 44168 0.29% 146 44720 0.29% 147 45193 0.30% 148 47025 0.31% 149 46365 0.30% 150 47755 0.31% 151 13669780 89.39% 15292298 reads passed initial QC criterion=sequence-density sequence-density=0.60 sequence-density-rank=1 fanout-score=2.02 fanout-score-rank=17 prefix-density=0.60 prefix-fanout=2.0 sequence=CGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTT criterion=fanout-score sequence-density=0.01 sequence-density-rank=22 fanout-score=480.70 fanout-score-rank=1 prefix-density=0.26 prefix-fanout=14.8 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGAGTATGAATGTGTTCTCTGTG criterion=sequence-density sequence-density=0.96 sequence-density-rank=1 fanout-score=2.04 fanout-score-rank=17 prefix-density=0.97 prefix-fanout=2.0 sequence=AACCGCACCCCGGCACAGGCCAACATGGTTGCACCATTCAACGGCCTCAAGTCTACCTCAGCTTTCCCGGTCACCAGAAAGGC criterion=fanout-score sequence-density=0.17 sequence-density-rank=19 fanout-score=21.28 fanout-score-rank=1 prefix-density=0.70 prefix-fanout=5.0 sequence=AATGGCAGCCTCAGTTATGGCTTCACTGAACCTGAAACCATCTCCATTCACGGTTGAGAAGTCTTCAGTGAGAGGCCTCCCAACTCTTTCAAGGAGATCTTTCAAGATT SRR13695461 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 04:08:19 Started mapping on | Feb 12 04:08:19 Finished on | Feb 12 04:10:24 Mapping speed, Million of reads per hour | 440.42 Number of input reads | 15292298 Average input read length | 296 UNIQUE READS: Uniquely mapped reads number | 14165326 Uniquely mapped reads % | 92.63% Average mapped length | 295.53 Number of splices: Total | 13760521 Number of splices: Annotated (sjdb) | 13482380 Number of splices: GT/AG | 13478857 Number of splices: GC/AG | 223254 Number of splices: AT/AC | 8346 Number of splices: Non-canonical | 50064 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 2.88 Insertion rate per base | 0.02% Insertion average length | 2.35 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 390029 % of reads mapped to multiple loci | 2.55% Number of reads mapped to too many loci | 38125 % of reads mapped to too many loci | 0.25% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.47% % of reads unmapped: other | 0.10% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 737113 737113 737113 N_multimapping 390029 390029 390029 N_noFeature 434699 13814192 648629 N_ambiguous 236332 1272 98201 UnstrandedReadsAssigned:13494295 PositiveStrandReadsAssigned:349862 NegativeStrandReadsAssigned:13418496 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695461 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695461-trimmed-pair1.fastq SRR13695461-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 15,292,298 reads, 13,458,509 reads pseudoaligned [quant] estimated average fragment length: 252.988 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,037 rounds 52401 SRR13695461.ke.tsv 34699 SRR13695461.se.tsv 87100 total ==> SRR13695461.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1766.01 477 17.7168 Potri.005G024800.1.v4.1 1035 783.012 167 13.9897 Potri.004G059700.1.v4.1 961 709.11 34 3.14503 Potri.007G009000.2.v4.1 1416 1164.01 0 0 Potri.003G141000.2.v4.1 2943 2691.01 687 16.7456 Potri.016G087400.1.v4.1 270 83.5065 720 565.552 Potri.015G069301.1.v4.1 564 321.726 0 0 Potri.010G195200.1.v4.1 1773 1521.01 32 1.38 Potri.012G127500.1.v4.1 977 725.069 225 20.3547 ==> SRR13695461.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 641 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 147 Potri.001G212900.v4.1 36 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 1 Potri.001G416900.v4.1 1 Potri.001G452600.v4.1 1 SRR13695461 completed mapping pipeline successfully