Starting /dee2/code/volunteer_pipeline.sh SRR13695462 current disk space = 3049186488320 free memory = 1035657280 SRR13695462 SRAfilesize 9c551336550e7d9735825a0a2b90ef0d SRR13695462.sra SRR13695462.sra file validated SRR13695462 is paired end SRR13695462 is conventional basespace SRR13695462 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695462_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.581 37.0 37.0 37.0 37.0 37.0 2 36.1595 37.0 37.0 37.0 37.0 37.0 3 36.523 37.0 37.0 37.0 37.0 37.0 4 36.575 37.0 37.0 37.0 37.0 37.0 5 36.6115 37.0 37.0 37.0 37.0 37.0 6 36.526 37.0 37.0 37.0 37.0 37.0 7 36.4475 37.0 37.0 37.0 37.0 37.0 8 36.577 37.0 37.0 37.0 37.0 37.0 9 36.4905 37.0 37.0 37.0 37.0 37.0 10-14 36.5382 37.0 37.0 37.0 37.0 37.0 15-19 36.4969 37.0 37.0 37.0 37.0 37.0 20-24 36.4985 37.0 37.0 37.0 37.0 37.0 25-29 36.4404 37.0 37.0 37.0 37.0 37.0 30-34 36.421299999999995 37.0 37.0 37.0 37.0 37.0 35-39 36.3391 37.0 37.0 37.0 37.0 37.0 40-44 36.4085 37.0 37.0 37.0 37.0 37.0 45-49 36.334900000000005 37.0 37.0 37.0 37.0 37.0 50-54 36.3214 37.0 37.0 37.0 37.0 37.0 55-59 36.3452 37.0 37.0 37.0 37.0 37.0 60-64 36.284000000000006 37.0 37.0 37.0 37.0 37.0 65-69 36.2717 37.0 37.0 37.0 37.0 37.0 70-74 36.2755 37.0 37.0 37.0 37.0 37.0 75-79 36.2341 37.0 37.0 37.0 37.0 37.0 80-84 36.162800000000004 37.0 37.0 37.0 37.0 37.0 85-89 36.184999999999995 37.0 37.0 37.0 37.0 37.0 90-94 36.1633 37.0 37.0 37.0 37.0 37.0 95-99 36.063300000000005 37.0 37.0 37.0 37.0 37.0 100-104 36.0578 37.0 37.0 37.0 37.0 37.0 105-109 36.1389 37.0 37.0 37.0 37.0 37.0 110-114 36.0173 37.0 37.0 37.0 37.0 37.0 115-119 36.0579 37.0 37.0 37.0 37.0 37.0 120-124 35.942699999999995 37.0 37.0 37.0 37.0 37.0 125-129 35.9419 37.0 37.0 37.0 37.0 37.0 130-134 35.8882 37.0 37.0 37.0 37.0 37.0 135-139 35.796 37.0 37.0 37.0 37.0 37.0 140-144 35.7513 37.0 37.0 37.0 37.0 37.0 145-149 35.4893 37.0 37.0 37.0 37.0 37.0 150-151 35.425 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 23 1.0 24 2.0 25 5.0 26 5.0 27 9.0 28 16.0 29 18.0 30 35.0 31 28.0 32 41.0 33 89.0 34 160.0 35 342.0 36 2916.0 37 333.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.1 11.85 6.4 36.65 2 20.718232044198896 13.008538422903063 36.5143144148669 29.758915118031144 3 17.05 16.950000000000003 27.375 38.625 4 23.3 22.575 25.074999999999996 29.049999999999997 5 25.224999999999998 29.9 24.7 20.175 6 20.625 34.300000000000004 23.724999999999998 21.349999999999998 7 15.024999999999999 27.250000000000004 39.25 18.475 8 15.925 26.150000000000002 34.375 23.549999999999997 9 17.375 23.025000000000002 35.875 23.724999999999998 10-14 20.185 29.585 27.169999999999998 23.06 15-19 20.075000000000003 28.194999999999997 28.475 23.255 20-24 20.06 29.125 27.395000000000003 23.419999999999998 25-29 19.905 28.54 28.51 23.044999999999998 30-34 20.205000000000002 28.51 28.599999999999998 22.685 35-39 19.59 28.665000000000003 28.01 23.735 40-44 20.995 28.625 27.41 22.97 45-49 20.385 28.28 27.48 23.855 50-54 20.525 28.449999999999996 27.800000000000004 23.225 55-59 19.900000000000002 28.189999999999998 28.285 23.625 60-64 20.645 27.67 28.49 23.195 65-69 20.445 28.485 27.875 23.195 70-74 19.2 29.125 28.194999999999997 23.48 75-79 21.04 27.71 27.845 23.405 80-84 19.939999999999998 28.249999999999996 28.194999999999997 23.615 85-89 20.11 29.17 27.87 22.85 90-94 20.435 28.895 28.065 22.605 95-99 19.84 27.555000000000003 28.48 24.125 100-104 21.05 28.975 27.125 22.85 105-109 21.58 28.315 27.589999999999996 22.515 110-114 20.275000000000002 28.615000000000002 27.55 23.56 115-119 21.154999999999998 28.84 27.07 22.935 120-124 20.48 28.935 27.355 23.23 125-129 20.835 28.04 27.255000000000003 23.87 130-134 21.175 28.625 27.13 23.07 135-139 20.905 28.475 27.169999999999998 23.45 140-144 20.965 27.215 27.55 24.27 145-149 20.925 28.310000000000002 26.91 23.855 150-151 21.3 28.537499999999998 26.400000000000002 23.7625 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 0.0 17 0.0 18 0.0 19 0.0 20 1.0 21 1.0 22 1.0 23 3.0 24 2.5 25 1.5 26 3.0 27 7.0 28 9.5 29 13.0 30 23.5 31 26.0 32 35.0 33 52.0 34 65.5 35 79.0 36 81.5 37 98.0 38 143.5 39 168.5 40 204.0 41 233.5 42 229.0 43 240.0 44 254.5 45 265.5 46 264.5 47 234.5 48 216.5 49 212.5 50 179.5 51 148.5 52 114.5 53 89.5 54 79.5 55 54.0 56 37.0 57 32.0 58 25.0 59 19.5 60 15.5 61 12.0 62 7.0 63 5.0 64 3.0 65 0.0 66 4.0 67 4.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.44999999999999996 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.10000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 69.67213114754098 46.75 2 19.262295081967213 25.85 3 6.929955290611028 13.950000000000001 4 2.1982116244411327 5.8999999999999995 5 1.1549925484351715 3.875 6 0.48435171385991055 1.95 7 0.07451564828614009 0.35000000000000003 8 0.07451564828614009 0.4 9 0.037257824143070044 0.22499999999999998 >10 0.11177347242921014 0.75 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGTTGTTTTACTTGGCCTTTCTGGGCCTAGTGATTGAAAACTTTTTCCAA 10 0.25 No Hit GCCCGATTCAGCATCCGAATCCAGAAGCTAAAAACAAAAACAAAGTAGAA 10 0.25 No Hit GAGCATCACATGATATGGAATCAAAAAAGTCATCCTTCACATAAACAGGC 10 0.25 No Hit GCTCTTCAAGGGTTCAACTTGATTGACCAGATGAGTGAAGTCGTTTATGA 9 0.22499999999999998 No Hit CTTCTTTCCAACACTAATAATAGTATAGTCAATTCCAAGCTGCTTCAAGT 8 0.2 No Hit GTGCGTCAATGAATGAATGACTGGAATAAGTTCAAGCACCAGGAGTGTTA 8 0.2 No Hit TCCGCCGCCACATTCTCTTCTAATTCTGCTCTCTCTTCCTCATCTTGGAC 7 0.17500000000000002 No Hit CACATCATGACTGAAAGCATGAGTGGAACACCTGAAACCACTCAACAACC 7 0.17500000000000002 No Hit GTATGGTGAAGGTGCATCAGATCCATACAAGTCCCATTGCCCAGTAGTGT 6 0.15 No Hit GGAGCAATCATAATAGGCATTGATATTTTGAAGCCCAAAACAGTGGTGGC 6 0.15 No Hit GTCTCACTAAGCTGTGTATTAACCTCAACAATCTCACCGGAGATCGGAGA 6 0.15 No Hit GGGGTTATGAGTAGGGATGAGCATAAACCAACAACTCTCAAAGAAGATGG 6 0.15 No Hit GCGTACTGTATCTCCCTTGTTTTATTCTTTTGCGTGTTTCCATAAAAAGA 6 0.15 No Hit CGTTGATCGGGAGTGCCTGAAATAAGAAGAGAGTAATCTACGACAGGAAT 6 0.15 No Hit CATGCATGGCTCTTGTCGCACAACATAGGAGAGTACCATGACAAGGACTT 6 0.15 No Hit CCCGGTTTGAAGTTGAAAAATTTGATTGTCAATTTCCTGCACAGTCAGTA 6 0.15 No Hit CACCACCTGTGCCATTTGTTTGAAGCCTTGATGACAAAAGAACAAGTTCT 6 0.15 No Hit GTCCTGGACCAAGTTCTCATTCTGCTTCAACAATTCATTTAAGTAGTCCA 6 0.15 No Hit CTCTAGTTGATTGTCTATTAGCAACACTATAACCACCAACATAACCAGCA 6 0.15 No Hit CCCAGCCATCAAGACAAAAAGAATCACACCACATGACCACAAATCTGCCT 6 0.15 No Hit GACGCATCAACCCCACTTGGAATAGAATCCTCGTCGAAGACAATGTTGTG 6 0.15 No Hit CAACAGGATCGAATGCCTTGGGGTCAGAAGATGAGGCGGTTTGTAATTCA 5 0.125 No Hit CTGCAGCAGGTAGTTGATGGCGGTGAAGAGGACGAGATTGCTGGTAGGCA 5 0.125 No Hit GTCCTTTATATGCTGGATTTGGTATCTTAGGTGATTTCCATAGACCACTC 5 0.125 No Hit CCGCTGTGTAAAATATATAATCTAGGGCACCGATAAAGTCTCTAGTGCAA 5 0.125 No Hit GCCTTCTAGATCCTTCAAATATTTTCAATTTTTTTCTTTTTTTGTGAACT 5 0.125 No Hit AACGTTGTCATAATAACCTCGACGAGATAATTCAAGAAAGTTCCTGCAAG 5 0.125 No Hit CTACGAAGCAAAGTTTGATGGATGATCCATGGGAAACTTATTGATCACAA 5 0.125 No Hit CGCAGAATGGAAAGAAACAGGTTCAGAATGTCCAAATAGAGAGCAGCCGA 5 0.125 No Hit CCAACATTTTGATCGCATCATTTCCAACACTGTCCGATACATCTTCAGCA 5 0.125 No Hit CCATCTACTACCTTAGTAAGAAAATCTAGCCCTTGGGAAAGTTTTTCTGC 5 0.125 No Hit ATCTCCTCTCAAACTCAGCAGCAGTAAGTAACCGATTTGAACCAACTTCA 5 0.125 No Hit GCTACATATTCACATGCAGGCACATATTATTTTACAAGCCCGATTACCAT 5 0.125 No Hit AGCCAGGTGAGTTGCCAAGTTCTCCAATGGTCCAGTACCAGGATAAGCTG 5 0.125 No Hit TCTTGAGTTCCTTCACCTTCAACTCAGCGAATGCTTCGGGATCGTCAGCC 5 0.125 No Hit GCCATAACAACCTTAACTCCATGAGCATGAGCATTCTTTATAAACTCTCC 5 0.125 No Hit GTACACTAGCTCTTCAACTTCACTAAACCTGTTCATTTGAAGTCCTTCCA 5 0.125 No Hit TGGGGACACGTGGATTCTCCTCCTTGTTGTACATGCCGGGAAGTTGCACA 5 0.125 No Hit CGTTCAGCGGATTCTGTAACTGCTCACAACACCACCTGCAGAAAAAATCT 5 0.125 No Hit GTCCTATTCCCCAGAAATTCAAAATCAAGCTCATCTCGAACGGCATCGGT 5 0.125 No Hit GTGGCAATCAGCTGAGCAATGATAAAAGCCACCACAAGTAATAACCCAGG 5 0.125 No Hit GGCCTGTTTAACCCTGACTATATCTGGATTGAGAATTCAACTGGATACTG 5 0.125 No Hit GTGGATAAGGGAGGAGGACACCCTTCTGGATGTATTGGCACGAGAGCATG 5 0.125 No Hit GTCCGTTGTCTTGAGAAATGGCAGCATAGCGGGCAAGACCCCAGGCAGCC 5 0.125 No Hit CGCATTGCAGTCCGAGCATCTCTAATATCATAGTAAGATATCATCACAAA 5 0.125 No Hit CTCAGGCTTGCTCTTGGTAACACTTAGGGTGATTTTACCTGTTGATGATG 5 0.125 No Hit GGTGAATTAAGTGGTTTGGGGGGCTCTGTTTGTTTAACAGAAGAAGCTAA 5 0.125 No Hit TCATCACCAGCACCATCATCTTCCTCATCAAGACCTCCAGCAATACCTTC 5 0.125 No Hit CTGAGCAAAATGGTTGCGGACCTCCATGTAATGGAATCTGAGAATGAGGG 5 0.125 No Hit GCTCATCAGGTTTATTAGTTTCCACAGAATCAAAACCTTTTGACAACTTA 5 0.125 No Hit CCTTTCACTGTGACTTTTTGCTCCTTCAAATCAATGTCATATGATTCCAC 5 0.125 No Hit CCCCGATTGACAGTGGTTTCTACAGCTTTCATGGCTTCAAATCTTGGCTC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.125 0.0 0.0 0.0 0.0 82-83 0.1375 0.0 0.0 0.0 0.0 84-85 0.2375 0.0 0.0 0.0 0.0 86-87 0.25 0.0 0.0 0.0 0.0 88-89 0.3375 0.0 0.0 0.0 0.0 90-91 0.4375 0.0 0.0 0.0 0.0 92-93 0.5249999999999999 0.0 0.0 0.0 0.0 94-95 0.625 0.0 0.0 0.0 0.0 96-97 0.925 0.0 0.0 0.0 0.0 98-99 1.1 0.0 0.0 0.0 0.0 100-101 1.1875 0.0 0.0 0.0 0.0 102-103 1.35 0.0 0.0 0.0 0.0 104-105 1.5625 0.0 0.0 0.0 0.0 106-107 1.7999999999999998 0.0 0.0 0.0 0.0 108-109 2.0625 0.0 0.0 0.0 0.0 110-111 2.375 0.0 0.0 0.0 0.0 112-113 2.6625 0.0 0.0 0.0 0.0 114-115 2.7874999999999996 0.0 0.0 0.0 0.0 116-117 3.0875 0.0 0.0 0.0 0.0 118-119 3.3499999999999996 0.0 0.0 0.0 0.0 120-121 3.5999999999999996 0.0 0.0 0.0 0.0 122-123 3.9125 0.0 0.0 0.0 0.0 124-125 4.325 0.0 0.0 0.0 0.0 126-127 4.7375 0.0 0.0 0.0 0.0 128-129 5.125 0.0 0.0 0.0 0.0 130-131 5.4125 0.0 0.0 0.0 0.0 132-133 5.7125 0.0 0.0 0.0 0.0 134-135 6.1625 0.0 0.0 0.0 0.0 136-137 6.612500000000001 0.0 0.0 0.0 0.0 138-139 6.9625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCACGAA 10 0.006830828 145.0 1 >>END_MODULE SRR13695462 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695462_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.01275 37.0 37.0 37.0 37.0 37.0 2 36.0145 37.0 37.0 37.0 37.0 37.0 3 36.017 37.0 37.0 37.0 37.0 37.0 4 36.17 37.0 37.0 37.0 37.0 37.0 5 36.219 37.0 37.0 37.0 37.0 37.0 6 36.219 37.0 37.0 37.0 37.0 37.0 7 36.2915 37.0 37.0 37.0 37.0 37.0 8 36.24 37.0 37.0 37.0 37.0 37.0 9 36.122 37.0 37.0 37.0 37.0 37.0 10-14 36.186499999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.1676 37.0 37.0 37.0 37.0 37.0 20-24 36.075900000000004 37.0 37.0 37.0 37.0 37.0 25-29 36.0851 37.0 37.0 37.0 37.0 37.0 30-34 35.97365 37.0 37.0 37.0 37.0 37.0 35-39 35.983799999999995 37.0 37.0 37.0 37.0 37.0 40-44 36.019600000000004 37.0 37.0 37.0 37.0 37.0 45-49 35.9915 37.0 37.0 37.0 37.0 37.0 50-54 35.9339 37.0 37.0 37.0 37.0 37.0 55-59 35.9019 37.0 37.0 37.0 37.0 37.0 60-64 35.82809999999999 37.0 37.0 37.0 37.0 37.0 65-69 35.78 37.0 37.0 37.0 37.0 37.0 70-74 35.767300000000006 37.0 37.0 37.0 37.0 37.0 75-79 35.7556 37.0 37.0 37.0 37.0 37.0 80-84 35.7802 37.0 37.0 37.0 37.0 37.0 85-89 35.69205000000001 37.0 37.0 37.0 37.0 37.0 90-94 35.67 37.0 37.0 37.0 37.0 37.0 95-99 35.675850000000004 37.0 37.0 37.0 37.0 37.0 100-104 35.63539999999999 37.0 37.0 37.0 37.0 37.0 105-109 35.6286 37.0 37.0 37.0 37.0 37.0 110-114 35.5265 37.0 37.0 37.0 37.0 37.0 115-119 35.44154999999999 37.0 37.0 37.0 37.0 37.0 120-124 35.4076 37.0 37.0 37.0 37.0 37.0 125-129 35.42 37.0 37.0 37.0 37.0 37.0 130-134 35.3283 37.0 37.0 37.0 34.6 37.0 135-139 35.37005 37.0 37.0 37.0 37.0 37.0 140-144 35.287099999999995 37.0 37.0 37.0 34.6 37.0 145-149 35.08545 37.0 37.0 37.0 27.4 37.0 150-151 34.9255 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 4.0 14 6.0 15 6.0 16 2.0 17 3.0 18 0.0 19 2.0 20 2.0 21 0.0 22 5.0 23 9.0 24 8.0 25 7.0 26 14.0 27 9.0 28 13.0 29 11.0 30 29.0 31 40.0 32 51.0 33 123.0 34 201.0 35 663.0 36 2620.0 37 170.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.70639899623588 25.520702634880806 10.388958594730239 22.383939774153074 2 28.999999999999996 25.15 29.549999999999997 16.3 3 21.25 28.599999999999998 31.4 18.75 4 23.95 34.225 24.775 17.05 5 26.575 37.125 20.549999999999997 15.75 6 22.3 39.825 20.45 17.424999999999997 7 20.3 24.625 37.225 17.849999999999998 8 22.15 26.174999999999997 25.5 26.174999999999997 9 21.575 25.0 30.475 22.95 10-14 24.099999999999998 29.785 26.009999999999998 20.105 15-19 23.244999999999997 28.244999999999997 27.675 20.835 20-24 22.693616169701823 29.972983790274167 26.94616770062037 20.387232339403642 25-29 23.38838838838839 28.06806806806807 27.267267267267272 21.276276276276278 30-34 22.71203402551914 28.956717538153615 27.72579434575932 20.605454090567925 35-39 22.948769261556933 27.9467680608365 28.086852111266758 21.017610566339805 40-44 23.273618895116094 28.412730184147318 26.946557245796637 21.36709367493995 45-49 22.685880116081254 28.019613729610725 27.83448413889723 21.460022015410786 50-54 22.75865519311587 28.48208925355213 27.0162097258355 21.7430458274965 55-59 23.133133133133132 27.852852852852855 27.82782782782783 21.186186186186188 60-64 21.758054832899738 28.607164298579146 28.126876125675405 21.507904742845707 65-69 22.81184355306592 28.178453536060815 27.608282484745423 21.40142042612784 70-74 22.892892892892895 27.362362362362365 27.71271271271271 22.03203203203203 75-79 23.22357886309047 28.12750200160128 27.692153722978386 20.956765412329865 80-84 22.316158079039518 28.494247123561784 28.269134567283643 20.920460230115058 85-89 23.121965867574197 27.76137330463941 27.656273459786796 21.4603873679996 90-94 23.414048429057434 27.176305783470085 28.001801080648388 21.407844706824093 95-99 22.54239831907549 28.33558457151433 28.015408474661065 21.106608634749115 100-104 23.73873873873874 28.453453453453452 27.56756756756757 20.24024024024024 105-109 23.773773773773772 28.303303303303302 26.576576576576578 21.346346346346344 110-114 24.13448068841305 27.556533920352212 27.366419851911143 20.942565539323592 115-119 24.027826435113358 27.901506431109553 27.536159351383816 20.534507782393273 120-124 24.454454454454456 27.717717717717715 27.952952952952952 19.874874874874877 125-129 23.916524872385146 28.97107396656991 26.55389850865779 20.558502652387148 130-134 23.961357493242566 29.011913104414855 26.65932525778356 20.367404144559014 135-139 23.729916412232843 28.274688422843987 27.38375294058762 20.61164222433555 140-144 24.259555733440063 28.311987192315392 27.001200720432262 20.427256353812286 145-149 24.22543670854397 29.09555032784424 26.582912057660547 20.096100905951246 150-151 25.06883604505632 28.64831038798498 26.608260325406757 19.67459324155194 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.0 6 0.0 7 1.0 8 2.0 9 1.0 10 1.0 11 1.5 12 1.0 13 0.5 14 0.0 15 0.5 16 1.0 17 0.5 18 0.5 19 0.5 20 0.5 21 1.0 22 2.0 23 2.5 24 2.0 25 2.5 26 3.0 27 4.0 28 6.5 29 9.0 30 14.0 31 20.0 32 26.0 33 52.0 34 63.5 35 54.5 36 79.0 37 114.0 38 141.0 39 165.5 40 209.5 41 243.5 42 257.0 43 266.5 44 250.0 45 226.5 46 234.5 47 268.0 48 233.5 49 187.0 50 190.0 51 163.0 52 105.5 53 76.0 54 78.5 55 65.0 56 40.0 57 26.0 58 21.5 59 19.5 60 18.5 61 15.0 62 6.5 63 3.5 64 3.5 65 1.5 66 0.0 67 1.0 68 3.0 69 2.5 70 0.5 71 0.0 72 0.0 73 0.5 74 0.5 75 0.5 76 1.0 77 0.5 78 0.0 79 0.0 80 0.0 81 0.5 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 1.0 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.375 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.06 25-29 0.1 30-34 0.075 35-39 0.06 40-44 0.08 45-49 0.06999999999999999 50-54 0.06 55-59 0.1 60-64 0.06 65-69 0.03 70-74 0.1 75-79 0.08 80-84 0.05 85-89 0.095 90-94 0.06 95-99 0.055 100-104 0.1 105-109 0.1 110-114 0.06 115-119 0.095 120-124 0.1 125-129 0.09 130-134 0.11 135-139 0.105 140-144 0.06 145-149 0.105 150-151 0.125 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.425 #Duplication Level Percentage of deduplicated Percentage of total 1 70.15202076381163 47.3 2 18.909899888765295 25.5 3 6.674082313681868 13.5 4 2.261772339636633 6.1 5 1.260659992584353 4.25 6 0.482017055988135 1.95 7 0.11123470522803114 0.525 8 0.07415647015202076 0.4 9 0.03707823507601038 0.22499999999999998 >10 0.03707823507601038 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TAGAGGAGGACGTGGAAGGGGAAGAGAAAATGAGATTTCACGTTCTGCTA 10 0.25 No Hit CACAAATTAAGGCTTAAAGATATAGAGAGAAAGAAACAACATGTCGTCGA 9 0.22499999999999998 No Hit GTTGAAGTTCTTTATAACATCAATGAACAGCTTCAGACTGATGATGTTGA 8 0.2 No Hit AATTGCTTGTTAGTCAAATGTCCTCTTTAGTTTGACCTTTGATCCAGCTG 8 0.2 No Hit GGCTTATGCAGACTTTTATGAAAAGGGCTCATTATATGGGGATTCCACCT 7 0.17500000000000002 No Hit CTTCCATTTCTGAATTCTCTGGTCGTTCCCATCAAGGCTTTCGTCGTGCT 7 0.17500000000000002 No Hit AGAAAAGGCGTGGGCTGCATTCTAGCATTGAATGCTAACCTAGACTTCTC 7 0.17500000000000002 No Hit GTTTTTCTTGAGAGTAGGCAAACAATAGAGCAAGTTTACAAGTTCTTTGG 6 0.15 No Hit GCCTGAACGGTCCTCTCTCGCTTTCTCTCCGATTCGTAACAGGTTTTCCG 6 0.15 No Hit ATCAAGGCCTCAATGAAAGACGTTGGTGCTGCCGTTGTCGCCACCGCTGC 6 0.15 No Hit CGGGAACCAAAATCAAGGCTATGGCATCACTAGCAACCTTTGCTGCAGTG 6 0.15 No Hit TATGAACCACATGATTTATGATTACTGTGCCGACAAATCCCGTTATCCGA 6 0.15 No Hit ATGTGTCCCTAGCTATATCTGTTAGGGCCTTAGGGGTGGTTTGTTTAAAT 6 0.15 No Hit GGTACCATTGAATTTGGTTTCTGAGTCCAAATGCTGATTATATGACTGCA 6 0.15 No Hit ATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATAGGAG 6 0.15 No Hit GAAGCGAGAAAGTATTTTCAGCAACTTATCAATGCCGTGGACTACTGCCA 6 0.15 No Hit TATGCTTCTGGGGCAGAGGACCAGTGGACTCTAGCAGAAAACAGAAATGC 6 0.15 No Hit AAAGAACTCCTTGCAATTGAATGGCCTTCTGTTCATTTACAAAATGAAGA 6 0.15 No Hit TCTTTGCATCCAACACAGTTGCTGCACCACATTCAGTGCAAATGTCCCAA 6 0.15 No Hit CTCACTTCCTCCCCCTCTCTCAATGGCTGCTGAAACAGCTTCCCTTCTTC 6 0.15 No Hit GTTTGTTCTGCCCTCCCACCTAAAAAAATCCCAAAAAAATTCTCCTTTTC 5 0.125 No Hit AACAATACTCTTAATATCACTTTTTGTAACACAAAACTCTAATGATCATG 5 0.125 No Hit GAGTTATATGGTTTTCTTCGGTGGTGTCATTAGTGCAACATCTCTCCCTC 5 0.125 No Hit ACCTAGCCCATGCTTGGCTGTTGGGCTCTTGCACACGCAAAACCCCCCAT 5 0.125 No Hit AAAAGAAAAGAAAAAATAGCAATAACAAAATTATTGAAGATGAAGAAGGC 5 0.125 No Hit CTGGTGAACCCGCAAAGCTTATCAGGCAAAGGTACCGTGAGGCGGCTGAT 5 0.125 No Hit AGTTTCTGGTCTTCAGGCAAAAGAGCAAGAAGCTGTGAAAAAGGATGCAG 5 0.125 No Hit AGGGAGCACTGCATAGCTTATAAGCTTGTAAGAGATGGCTTCCTCCTCTA 5 0.125 No Hit GTGCGGAAAGATGACGGCGGATGCAGAAGGTGGAGCTCCAGAGGTTACTC 5 0.125 No Hit ATTCTCTATCCACCTCGGAGAGGCGAAGAAATGGGAAAGGACTACAACGA 5 0.125 No Hit TGGAAATTTGCTTCCTGATGATGATGAGCTTCTTGCTGGCATAATGGATG 5 0.125 No Hit CCCTGGAGAAAACAGCTCACGATGACTTCCGAAAGCTTGCATCAGCAATT 5 0.125 No Hit GCCTTTGTTGAGCACCAACGTAGCATGGAGAAAGACACAGAGAAGAAGAA 5 0.125 No Hit ACCACATATAGAGGGTGTAATAGCTAAGTAGCCTGTAAGAGATGGCTTCC 5 0.125 No Hit CGGGGAAGGGTGATCCTGGCAATACGTCTTCCAATGAAGTGGAAGTTGAG 5 0.125 No Hit AGTAGACCCTCTTGGAATCTTACGGCCTCACAACAAGCTTACACATCAGC 5 0.125 No Hit ATACAGACTGGGATATACTTCCTCCCCGGAAAATTAAAGCTGTCAAGGCG 5 0.125 No Hit ACCTAGCCCAAGCTTGGCTGTTGGGCTCTTGCACACGCAAAACCCCCCAT 5 0.125 No Hit CAGCAAAGCTCTAGTTTCTCCATAGATCTACTCTTCATGGATCATCCTTC 5 0.125 No Hit CTCATCGGCAGCCGTTGCCACCGTCAACCGCACCCCGGCACAAGCCAACA 5 0.125 No Hit CTGAAAACACGCCCCTTTGATGCTATTTTGGCTGAGGTGCGAGCATTCTT 5 0.125 No Hit TGAAGGGCTTCTTGACACGGACATGGTTGAAAGTGACTTATAGGCAATGG 5 0.125 No Hit GGGGATTATTATGTAATGGTTATGGACATGTTGGGCCCTAGCCTTTGGGA 5 0.125 No Hit GGTGGGTCTTCTGGAACGCCGAACAAGCCAATCTCTACGCCTCAAGCAAA 5 0.125 No Hit GAAGGAGTGGTCATTGAAAGGAGCAAGAAAGATGGCACCACACTAACAGT 5 0.125 No Hit AGAATTTATATCCCATGATCCAAGATGGTACGAGGCCTATTGAAGCTGAA 5 0.125 No Hit GGGCCCACACCGACGCTGGTGGCATCATCTTGCTATTCCAGGATGACAAA 5 0.125 No Hit CTTCAGTGGAGATTTCCTTGTACCATCCTACCGGGGCTCTTCTTTCCTGG 5 0.125 No Hit GGCAATTACGTCTCTGGTGCCATCCTTTTTGAGGAGACTCTCTACCAATC 5 0.125 No Hit CTGTATGGTTCAAGGCTGGAGCCCAGATCTTCAGCGAGGGTGGACTTGAC 5 0.125 No Hit GATGCACAAGAAGGTCTGGATTGCTGCGGGTGATATAATTCTCGTTGGCC 5 0.125 No Hit GTTCTGTTTATTACAGCAGCAATACCATAATCATGTCTCAGACTGTTGTC 5 0.125 No Hit CTCTTCACTGCCCTCATTATCCTCATCCTGACTAGTTTTATCCAGGTGTT 5 0.125 No Hit CAGTGATTGAAGATTAGTTAGGTGAATGGCTGATAGCTGAGGCACCATTA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.125 0.0 0.0 0.0 0.0 82-83 0.1375 0.0 0.0 0.0 0.0 84-85 0.2375 0.0 0.0 0.0 0.0 86-87 0.25 0.0 0.0 0.0 0.0 88-89 0.3375 0.0 0.0 0.0 0.0 90-91 0.4375 0.0 0.0 0.0 0.0 92-93 0.5249999999999999 0.0 0.0 0.0 0.0 94-95 0.625 0.0 0.0 0.0 0.0 96-97 0.925 0.0 0.0 0.0 0.0 98-99 1.1 0.0 0.0 0.0 0.0 100-101 1.2 0.0 0.0 0.0 0.0 102-103 1.375 0.0 0.0 0.0 0.0 104-105 1.5875 0.0 0.0 0.0 0.0 106-107 1.8250000000000002 0.0 0.0 0.0 0.0 108-109 2.0875 0.0 0.0 0.0 0.0 110-111 2.4000000000000004 0.0 0.0 0.0 0.0 112-113 2.6875 0.0 0.0 0.0 0.0 114-115 2.8125 0.0 0.0 0.0 0.0 116-117 3.1125 0.0 0.0 0.0 0.0 118-119 3.3499999999999996 0.0 0.0 0.0 0.0 120-121 3.5999999999999996 0.0 0.0 0.0 0.0 122-123 3.9125 0.0 0.0 0.0 0.0 124-125 4.3125 0.0 0.0 0.0 0.0 126-127 4.7 0.0 0.0 0.0 0.0 128-129 5.075 0.0 0.0 0.0 0.0 130-131 5.3625 0.0 0.0 0.0 0.0 132-133 5.6625 0.0 0.0 0.0 0.0 134-135 6.1 0.0 0.0 0.0 0.0 136-137 6.5375 0.0 0.0 0.0 0.0 138-139 6.8875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 718142 spots for SRR13695462.sra Written 718142 spots for SRR13695462.sra Read 718142 spots for SRR13695462.sra Written 718142 spots for SRR13695462.sra Read 718142 spots for SRR13695462.sra Written 718142 spots for SRR13695462.sra Read 718142 spots for SRR13695462.sra Written 718142 spots for SRR13695462.sra Read 718142 spots for SRR13695462.sra Written 718142 spots for SRR13695462.sra Read 718142 spots for SRR13695462.sra Written 718142 spots for SRR13695462.sra Read 718142 spots for SRR13695462.sra Written 718142 spots for SRR13695462.sra Read 718142 spots for SRR13695462.sra Written 718142 spots for SRR13695462.sra Read 718142 spots for SRR13695462.sra Written 718142 spots for SRR13695462.sra Read 718142 spots for SRR13695462.sra Written 718142 spots for SRR13695462.sra Read 718142 spots for SRR13695462.sra Written 718142 spots for SRR13695462.sra Read 718157 spots for SRR13695462.sra Written 718157 spots for SRR13695462.sra Read 718142 spots for SRR13695462.sra Written 718142 spots for SRR13695462.sra Read 718142 spots for SRR13695462.sra Written 718142 spots for SRR13695462.sra Read 718142 spots for SRR13695462.sra Written 718142 spots for SRR13695462.sra Read 718142 spots for SRR13695462.sra Written 718142 spots for SRR13695462.sra Read 718142 spots for SRR13695462.sra Written 718142 spots for SRR13695462.sra Read 718142 spots for SRR13695462.sra Written 718142 spots for SRR13695462.sra Read 718142 spots for SRR13695462.sra Written 718142 spots for SRR13695462.sra Read 718142 spots for SRR13695462.sra Written 718142 spots for SRR13695462.sra SRR ids: ['SRR13695462.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_y9od3nme SRR13695462.sra spots: 14362855 blocks: [[1, 718142], [718143, 1436284], [1436285, 2154426], [2154427, 2872568], [2872569, 3590710], [3590711, 4308852], [4308853, 5026994], [5026995, 5745136], [5745137, 6463278], [6463279, 7181420], [7181421, 7899562], [7899563, 8617704], [8617705, 9335846], [9335847, 10053988], [10053989, 10772130], [10772131, 11490272], [11490273, 12208414], [12208415, 12926556], [12926557, 13644698], [13644699, 14362855]] SRR13695462 file size 4859426 SRR13695462 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695462 SRR13695462_1.fastq SRR13695462_2.fastq Input file: SRR13695462_1.fastq Paired file: SRR13695462_2.fastq trimmed: SRR13695462-trimmed-pair1.fastq, SRR13695462-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 04:30:21 2025 >> started Wed Feb 12 04:30:36 2025 >> done (15.267s) 14362855 read pairs processed; of these: 115 ( 0.00%) short read pairs filtered out after trimming by size control 687 ( 0.00%) empty read pairs filtered out after trimming by size control 14362053 (99.99%) read pairs available; of these: 1601322 (11.15%) trimmed read pairs available after processing 12760731 (88.85%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 0 0.00% 20 1 0.00% 21 5 0.00% 22 3 0.00% 23 3 0.00% 24 2 0.00% 25 2 0.00% 26 1 0.00% 27 9 0.00% 28 1 0.00% 29 1 0.00% 30 5 0.00% 31 1 0.00% 32 14 0.00% 33 6 0.00% 34 5 0.00% 35 5 0.00% 36 7 0.00% 37 7 0.00% 38 7 0.00% 39 8 0.00% 40 5 0.00% 41 17 0.00% 42 5 0.00% 43 15 0.00% 44 15 0.00% 45 13 0.00% 46 7 0.00% 47 16 0.00% 48 30 0.00% 49 36 0.00% 50 38 0.00% 51 67 0.00% 52 42 0.00% 53 59 0.00% 54 57 0.00% 55 90 0.00% 56 70 0.00% 57 95 0.00% 58 134 0.00% 59 140 0.00% 60 183 0.00% 61 225 0.00% 62 239 0.00% 63 258 0.00% 64 276 0.00% 65 314 0.00% 66 412 0.00% 67 470 0.00% 68 531 0.00% 69 636 0.00% 70 711 0.00% 71 803 0.01% 72 998 0.01% 73 1157 0.01% 74 1386 0.01% 75 1436 0.01% 76 1602 0.01% 77 1747 0.01% 78 1935 0.01% 79 2304 0.02% 80 2405 0.02% 81 2806 0.02% 82 3132 0.02% 83 3606 0.03% 84 4050 0.03% 85 4597 0.03% 86 4749 0.03% 87 5139 0.04% 88 5412 0.04% 89 5806 0.04% 90 6146 0.04% 91 6999 0.05% 92 7387 0.05% 93 7834 0.05% 94 8514 0.06% 95 9487 0.07% 96 9791 0.07% 97 10007 0.07% 98 10720 0.07% 99 11187 0.08% 100 11671 0.08% 101 12114 0.08% 102 12747 0.09% 103 13797 0.10% 104 14231 0.10% 105 15196 0.11% 106 15989 0.11% 107 16347 0.11% 108 17178 0.12% 109 17701 0.12% 110 17571 0.12% 111 18608 0.13% 112 19131 0.13% 113 20063 0.14% 114 20852 0.15% 115 21740 0.15% 116 22608 0.16% 117 23317 0.16% 118 24193 0.17% 119 24269 0.17% 120 24569 0.17% 121 25948 0.18% 122 25787 0.18% 123 27177 0.19% 124 27996 0.19% 125 28623 0.20% 126 29573 0.21% 127 30265 0.21% 128 30390 0.21% 129 31553 0.22% 130 32103 0.22% 131 32354 0.23% 132 33165 0.23% 133 34288 0.24% 134 34497 0.24% 135 35079 0.24% 136 36228 0.25% 137 37426 0.26% 138 37418 0.26% 139 38467 0.27% 140 38418 0.27% 141 39519 0.28% 142 39735 0.28% 143 40394 0.28% 144 41061 0.29% 145 42213 0.29% 146 42305 0.29% 147 43104 0.30% 148 44280 0.31% 149 44278 0.31% 150 45344 0.32% 151 12760731 88.85% 14362053 reads passed initial QC criterion=sequence-density sequence-density=0.51 sequence-density-rank=1 fanout-score=2.01 fanout-score-rank=20 prefix-density=0.52 prefix-fanout=2.0 sequence=CGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTT criterion=fanout-score sequence-density=0.05 sequence-density-rank=23 fanout-score=11.81 fanout-score-rank=1 prefix-density=0.17 prefix-fanout=3.7 sequence=ACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAG criterion=sequence-density sequence-density=0.69 sequence-density-rank=1 fanout-score=2.14 fanout-score-rank=23 prefix-density=0.70 prefix-fanout=2.1 sequence=TGTAAGAGATGGCTTCCTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=26 fanout-score=88.36 fanout-score-rank=1 prefix-density=0.11 prefix-fanout=6.0 sequence=CAACTTCAAGGGCAGTAGTCTTAAACCATACTCTAAAATCTTCTTATAATTCCAGTTGTAATATTCTGCTAGCATATAATGGCTTCTTCAATGAGCTTGAAGCTGGCCTGTGCCATGCTTGTAGCGATGGTTGTTAGTGCACCACTAGCAGAAGCTGCCATCTCATGTGGCCAGGTGTCAAGCAGCTTGGCACAATGTATAACCTACCTCCAGAAGGGTGGGGCTGTGCCTGCAGCTTGCTGCAGTGGGTTGAAAGGACTTAATTCTGCAGCCACGACCACCGCCGACC SRR13695462 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 04:31:24 Started mapping on | Feb 12 04:31:25 Finished on | Feb 12 04:33:20 Mapping speed, Million of reads per hour | 449.59 Number of input reads | 14362053 Average input read length | 296 UNIQUE READS: Uniquely mapped reads number | 13237083 Uniquely mapped reads % | 92.17% Average mapped length | 295.01 Number of splices: Total | 12614017 Number of splices: Annotated (sjdb) | 12359942 Number of splices: GT/AG | 12361773 Number of splices: GC/AG | 208395 Number of splices: AT/AC | 7618 Number of splices: Non-canonical | 36231 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.03% Deletion average length | 2.88 Insertion rate per base | 0.02% Insertion average length | 2.42 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 367634 % of reads mapped to multiple loci | 2.56% Number of reads mapped to too many loci | 54580 % of reads mapped to too many loci | 0.38% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.73% % of reads unmapped: other | 0.17% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 757506 757506 757506 N_multimapping 367634 367634 367634 N_noFeature 489660 12930689 691447 N_ambiguous 188185 1271 82678 UnstrandedReadsAssigned:12559238 PositiveStrandReadsAssigned:305123 NegativeStrandReadsAssigned:12462958 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695462 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695462-trimmed-pair1.fastq SRR13695462-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 14,362,053 reads, 12,613,084 reads pseudoaligned [quant] estimated average fragment length: 251.437 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,061 rounds 52401 SRR13695462.ke.tsv 34699 SRR13695462.se.tsv 87100 total ==> SRR13695462.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1767.56 343 15.727 Potri.005G024800.1.v4.1 1035 784.563 92 9.50357 Potri.004G059700.1.v4.1 961 710.656 6 0.684255 Potri.007G009000.2.v4.1 1416 1165.56 0 0 Potri.003G141000.2.v4.1 2943 2692.56 608 18.3005 Potri.016G087400.1.v4.1 270 84.4894 790 757.794 Potri.015G069301.1.v4.1 564 323.369 0 0 Potri.010G195200.1.v4.1 1773 1522.56 8 0.425835 Potri.012G127500.1.v4.1 977 726.643 122 13.6071 ==> SRR13695462.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 684 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 244 Potri.001G212900.v4.1 1 Potri.001G182400.v4.1 1 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 1 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 4 SRR13695462 completed mapping pipeline successfully