Starting /dee2/code/volunteer_pipeline.sh SRR13695463 current disk space = 3049125216256 free memory = 1433273416 SRR13695463 SRAfilesize ced9c95dc335b30261be110aa830c650 SRR13695463.sra SRR13695463.sra file validated SRR13695463 is paired end SRR13695463 is conventional basespace SRR13695463 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695463_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.554 37.0 37.0 37.0 37.0 37.0 2 36.2435 37.0 37.0 37.0 37.0 37.0 3 36.6465 37.0 37.0 37.0 37.0 37.0 4 36.5525 37.0 37.0 37.0 37.0 37.0 5 36.6335 37.0 37.0 37.0 37.0 37.0 6 36.6315 37.0 37.0 37.0 37.0 37.0 7 36.463 37.0 37.0 37.0 37.0 37.0 8 36.5515 37.0 37.0 37.0 37.0 37.0 9 36.62 37.0 37.0 37.0 37.0 37.0 10-14 36.5675 37.0 37.0 37.0 37.0 37.0 15-19 36.546299999999995 37.0 37.0 37.0 37.0 37.0 20-24 36.53830000000001 37.0 37.0 37.0 37.0 37.0 25-29 36.4645 37.0 37.0 37.0 37.0 37.0 30-34 36.4856 37.0 37.0 37.0 37.0 37.0 35-39 36.42810000000001 37.0 37.0 37.0 37.0 37.0 40-44 36.45309999999999 37.0 37.0 37.0 37.0 37.0 45-49 36.41289999999999 37.0 37.0 37.0 37.0 37.0 50-54 36.405 37.0 37.0 37.0 37.0 37.0 55-59 36.388400000000004 37.0 37.0 37.0 37.0 37.0 60-64 36.4195 37.0 37.0 37.0 37.0 37.0 65-69 36.3296 37.0 37.0 37.0 37.0 37.0 70-74 36.347300000000004 37.0 37.0 37.0 37.0 37.0 75-79 36.271100000000004 37.0 37.0 37.0 37.0 37.0 80-84 36.2252 37.0 37.0 37.0 37.0 37.0 85-89 36.264700000000005 37.0 37.0 37.0 37.0 37.0 90-94 36.1781 37.0 37.0 37.0 37.0 37.0 95-99 36.1869 37.0 37.0 37.0 37.0 37.0 100-104 36.1915 37.0 37.0 37.0 37.0 37.0 105-109 36.1786 37.0 37.0 37.0 37.0 37.0 110-114 36.094100000000005 37.0 37.0 37.0 37.0 37.0 115-119 36.05 37.0 37.0 37.0 37.0 37.0 120-124 36.012600000000006 37.0 37.0 37.0 37.0 37.0 125-129 35.9819 37.0 37.0 37.0 37.0 37.0 130-134 36.0399 37.0 37.0 37.0 37.0 37.0 135-139 35.914300000000004 37.0 37.0 37.0 37.0 37.0 140-144 35.9385 37.0 37.0 37.0 37.0 37.0 145-149 35.69279999999999 37.0 37.0 37.0 37.0 37.0 150-151 35.5105 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 2.0 25 2.0 26 4.0 27 11.0 28 11.0 29 14.0 30 23.0 31 36.0 32 60.0 33 55.0 34 127.0 35 316.0 36 2943.0 37 396.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.175 11.85 8.575000000000001 41.4 2 20.80823293172691 14.307228915662652 35.6425702811245 29.24196787148594 3 18.975 19.1 26.025 35.9 4 23.925 22.975 22.175 30.925000000000004 5 25.15 29.25 24.875 20.724999999999998 6 20.7 36.1 21.925 21.275 7 15.55 28.475 39.800000000000004 16.175 8 17.95 24.9 33.675 23.474999999999998 9 16.950000000000003 25.174999999999997 34.575 23.3 10-14 18.87 30.070000000000004 27.76 23.3 15-19 20.235 28.29 27.325 24.15 20-24 19.55 28.294999999999998 27.76 24.395 25-29 20.84 28.804999999999996 27.045 23.31 30-34 20.265 29.075 27.955000000000002 22.705000000000002 35-39 21.46 28.22 27.37 22.95 40-44 20.355 28.315 27.57 23.76 45-49 20.165 27.55 27.72 24.565 50-54 20.32 28.935 27.315 23.43 55-59 19.355 29.265 27.584999999999997 23.794999999999998 60-64 20.09 29.325000000000003 26.545 24.04 65-69 20.685000000000002 28.095 27.42 23.799999999999997 70-74 20.575 28.425 27.279999999999998 23.72 75-79 19.79 29.375 27.02 23.815 80-84 20.84 28.665000000000003 26.93 23.565 85-89 20.549999999999997 28.015 27.98 23.455000000000002 90-94 21.175 27.445000000000004 28.065 23.315 95-99 19.88 27.765 28.055000000000003 24.3 100-104 21.490000000000002 27.73 27.41 23.369999999999997 105-109 21.14 28.225 27.605 23.03 110-114 21.01 28.215 27.279999999999998 23.494999999999997 115-119 21.445 27.694999999999997 27.625 23.235 120-124 21.65 27.67 27.169999999999998 23.51 125-129 21.099999999999998 28.235 27.42 23.244999999999997 130-134 21.515 27.93 27.150000000000002 23.405 135-139 21.709999999999997 27.165 27.339999999999996 23.785 140-144 21.93 27.315 27.310000000000002 23.445 145-149 21.805 27.22 26.974999999999998 24.0 150-151 20.7875 27.525 26.7125 24.975 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 1.0 24 2.0 25 2.0 26 8.5 27 14.0 28 10.5 29 9.5 30 20.0 31 26.5 32 24.5 33 31.0 34 42.0 35 74.5 36 97.5 37 109.5 38 130.5 39 145.5 40 173.0 41 213.5 42 251.5 43 249.0 44 227.0 45 230.5 46 260.5 47 266.0 48 247.5 49 229.5 50 188.5 51 149.5 52 133.5 53 106.5 54 74.0 55 62.0 56 52.0 57 40.5 58 29.0 59 19.0 60 17.5 61 10.5 62 4.5 63 7.0 64 3.0 65 0.0 66 2.0 67 2.5 68 0.5 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.475 #Duplication Level Percentage of deduplicated Percentage of total 1 69.87589319292967 46.45 2 17.90146671681083 23.799999999999997 3 7.484016547574276 14.924999999999999 4 2.557352388115833 6.800000000000001 5 1.4667168108311395 4.875 6 0.45129748025573524 1.7999999999999998 7 0.07521624670928921 0.35000000000000003 8 0.188040616773223 1.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTCTGCTTTGCCATTAGATCCCGTAAGGTTTGGGTGAGTAAATCATCCT 8 0.2 No Hit GTGCTAAATTCTTTCAATTGAATAAAAGAAACTGGCAAAAAGACACCCAG 8 0.2 No Hit CTCTGGTGATATCTTGTTGATCATCAGACATGTCTGTAACAAGGCCTCCC 8 0.2 No Hit CCCCTCTGTCCTTTTCGGTCTGCTTCTCCTCATCGTACTCCTCAGCAACA 8 0.2 No Hit GTTGTAAATTATCAATGGAGATTTGGCATATAACGAGACAATGCAACCAT 8 0.2 No Hit TAAACATAAGATTATCCTTAAAAATCTCAAATCTCTTTTCTTTCTCTCCT 7 0.17500000000000002 No Hit CTCCTGTTAGATTTGGATCATCACCGGTTAAACTCTTCTGTAGTGCAGCG 7 0.17500000000000002 No Hit TGGCGTGATCGGACTCGTCATCTCCTGAGTCCGTTTAGTAACTCAAGGAA 6 0.15 No Hit CCCCAATATCCTTTGGACACAGTGTGGAAAGAAACAAGCTGGACTTCCTT 6 0.15 No Hit CAGGCTCTTAATCCGATTGTAACTGCTATCCTTCTCATCATAATCAAGAT 6 0.15 No Hit GCCCGATTCAGCATCCGAATCCAGAAGCTAAAAACAAAAACAAAGTAGAA 6 0.15 No Hit ATCCAAACATGGGTAGTTTCCACATAGTCCAGTAGCGTCCATCATAGTAC 6 0.15 No Hit AGAGGACAATAAGCTTAATTAACATACTTGCTACAAAATATGTAATTGTA 6 0.15 No Hit CTGGCCAACAACATTGAACAATGTGGCTCGGTTTCCAGATCCCTTGAAGG 6 0.15 No Hit CACCAGACTTCTTGGCTGAGGTGGAACCCTCATCGACAACAGGAATCAGG 6 0.15 No Hit CTGATCCAATGGCTGGTTGAGGTCAACTTTAGAATGGGTTTTCTCTGTCA 6 0.15 No Hit CCAGTACCAAGGACTATTATCTGCACATTGTTTTTAACCAACATTGGAAT 6 0.15 No Hit TGGAAGTAATGTCATTAGCCTTTCTGGTACTGACTGGGAAAGCTGCGGCA 6 0.15 No Hit ACCACAAACAGAACACACTGCAAGTTAACATGCCATGACCAGGTCTAATC 6 0.15 No Hit GTGACAATTCAAGTTCTGAAATATGGATAAGAATGATGAAACCTGTGTGA 5 0.125 No Hit CTTCACTATACTTCTTCTCATACTCAGCAAACTTGGTGTTCCATTCGGCT 5 0.125 No Hit ATCCCCATCCTCGTCAACACACGATCCCAAGCAATGCACCACTCCTGCAC 5 0.125 No Hit CTCACGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCC 5 0.125 No Hit GTGGAATGGTAATTTTATAATGATCATTGAGGTTCACCGGTTAACGGATC 5 0.125 No Hit GCTTGCTTATGTTTTAATGGCTGATTTGAGTATGAGGTTCCTGCAATATC 5 0.125 No Hit GAATTATTTGAGACAGCATTTACCATAGCGCTTTAGCAGGCATCAAGATC 5 0.125 No Hit GCACAGTCTAAGGGAAGAAATACGACTTCACATCCCCTCACTAATAGTAA 5 0.125 No Hit GCCATCCCAGTAAAAACCTGTCGCCGGTTCGAACCGTCCCGGTCCACAAC 5 0.125 No Hit TGATGATTGGCAACAAGCTAGCTTGGGAGCTCTTTAGGCATCTGCTTGAA 5 0.125 No Hit GCCACTGCGAGTAGACAGGATTTTTCCGGAGTAAATTGGGGTCTCTTCAT 5 0.125 No Hit GGGCAGGTTGCTATCTGAGAATTCTCTCCGTTATCATTTGAGATTGAAAG 5 0.125 No Hit CCTGGAAGGCTGCCATTTAGATAAGATGGAGAAGGCAAACCAGGTAACCA 5 0.125 No Hit CTCAGCCTGTTCAGCATTCTTGGCCCCTGGGATTGGAACGACATTCTCCT 5 0.125 No Hit CATCAAATAAAAGTTGAAAATGAAACTAGAAATTCCATTATATAGGTCAA 5 0.125 No Hit CTCCTTCCAAAACAACTGCTAGCCTGCGAAGTCTACCAATTGACCTTGCT 5 0.125 No Hit TTCGGTTTAGGCTTCATAATTACAGGTAGCTTAGGTGGGCGGCCTGGTCG 5 0.125 No Hit CTTCCAAAGCTAGCCTATTCCAGAATGGTGGCAAATCTGTTGTAGAAACT 5 0.125 No Hit GGAGGTTTTCTTAATGCTACTTTCTTGTATGGAAGGTGGGGCGCTACCAA 5 0.125 No Hit CTTTCTTGGCTTCATCTGACTCTCCCTCACCGGTGTACTTGCCAAGCTGA 5 0.125 No Hit CATCCACTTTGCAACTGGAGACCGAATGCCATCACAGCCAATAACAATCT 5 0.125 No Hit TAAGAATGTAATCACACACATACATTGCCTCTTTCATATCCTTCACAATA 5 0.125 No Hit ATCGGCACTGGACACAGTGAATTCTCCACCCCCTTCAATATTTGCAGCTT 5 0.125 No Hit CCACAGTTTCCTTGCCAATGCTTCATCTCTAGCAAATGCACTGGGCTCCA 5 0.125 No Hit CCGGCACCAGTTTCACTGAAAAAGGTGTTAAAGGCATCATCCCCTCCACC 5 0.125 No Hit GTGTATGAGTGAGTGGTGGTCATGGTGCCCTTGATGATGCCAAACTTCTG 5 0.125 No Hit GTTTTCTCATCACCATTCACGTCCACAGTGTTGTCAACCACCAAATTCAT 5 0.125 No Hit CCCACCATCTTCAACCACTGTTGTCCATATAGAAACTAATTTTCAGAGTG 5 0.125 No Hit TCGGTAAACAAACAATTCTGCAGAAACAGCATTCCTTGGCTGGCTGGAAG 5 0.125 No Hit CACAGAACATGTCTACAGCCTGAACTAAGCATGAAATTGCCTCACTAGTG 5 0.125 No Hit AGGGGAAGTAATTGTAAATCTTCTTGTTAAGTATGTTAAATATCACGTTC 5 0.125 No Hit TGCACAAACTCCATCGAAGGAAAATAGTCTCCAAGACTGAATCCTCCAAG 5 0.125 No Hit CGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCA 5 0.125 No Hit AGCGAATGTGAAGTTTTTAAGATTGGAGAAAATAAAGCAGAAGATGATGA 5 0.125 No Hit GTTGTGCAGCAAAAGCAGGCTCACATGAGCTCAAGGTAGTGAAGATAGCA 5 0.125 No Hit CCAAGAACAACCATCGCCACTATTGCCCATGGAGGAGGTAACCAGTTATT 5 0.125 No Hit CCGAGAACATGGCAGCCCCCATCATACACTTTCTGGGATATCAATCCCAT 5 0.125 No Hit CCAACTGGTGAGCCACTGGTTCATCGAGTGTTTGATAATCGATCGGCTGA 5 0.125 No Hit CCTGTTCTCTGGTTTCATACGGTGGTGGAAGCAATCCCCGTACATTAGCA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0125 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.05 0.0 0.0 0.0 0.0 82-83 0.2125 0.0 0.0 0.0 0.0 84-85 0.2625 0.0 0.0 0.0 0.0 86-87 0.375 0.0 0.0 0.0 0.0 88-89 0.45 0.0 0.0 0.0 0.0 90-91 0.525 0.0 0.0 0.0 0.0 92-93 0.5875 0.0 0.0 0.0 0.0 94-95 0.7375 0.0 0.0 0.0 0.0 96-97 0.8 0.0 0.0 0.0 0.0 98-99 1.0625 0.0 0.0 0.0 0.0 100-101 1.3 0.0 0.0 0.0 0.0 102-103 1.7375 0.0 0.0 0.0 0.0 104-105 2.0875 0.0 0.0 0.0 0.0 106-107 2.4375 0.0 0.0 0.0 0.0 108-109 2.725 0.0 0.0 0.0 0.0 110-111 2.9875 0.0 0.0 0.0 0.0 112-113 3.4125 0.0 0.0 0.0 0.0 114-115 3.7 0.0 0.0 0.0 0.0 116-117 4.2875 0.0 0.0 0.0 0.0 118-119 5.0375 0.0 0.0 0.0 0.0 120-121 5.262499999999999 0.0 0.0 0.0 0.0 122-123 5.7125 0.0 0.0 0.0 0.0 124-125 6.475 0.0 0.0 0.0 0.0 126-127 7.0625 0.0 0.0 0.0 0.0 128-129 7.7625 0.0 0.0 0.0 0.0 130-131 8.2125 0.0 0.0 0.0 0.0 132-133 8.6875 0.0 0.0 0.0 0.0 134-135 9.2625 0.0 0.0 0.0 0.0 136-137 9.85 0.0 0.0 0.0 0.0 138-139 10.475 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGAAATA 10 0.006830828 145.0 3 >>END_MODULE SRR13695463 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695463_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.0635 37.0 37.0 37.0 37.0 37.0 2 36.04 37.0 37.0 37.0 37.0 37.0 3 36.0675 37.0 37.0 37.0 37.0 37.0 4 36.18 37.0 37.0 37.0 37.0 37.0 5 36.2095 37.0 37.0 37.0 37.0 37.0 6 36.205 37.0 37.0 37.0 37.0 37.0 7 36.0915 37.0 37.0 37.0 37.0 37.0 8 36.2855 37.0 37.0 37.0 37.0 37.0 9 36.2565 37.0 37.0 37.0 37.0 37.0 10-14 36.2161 37.0 37.0 37.0 37.0 37.0 15-19 36.2342 37.0 37.0 37.0 37.0 37.0 20-24 36.17765000000001 37.0 37.0 37.0 37.0 37.0 25-29 36.12814999999999 37.0 37.0 37.0 37.0 37.0 30-34 36.0509 37.0 37.0 37.0 37.0 37.0 35-39 36.04105 37.0 37.0 37.0 37.0 37.0 40-44 36.02395 37.0 37.0 37.0 37.0 37.0 45-49 36.0854 37.0 37.0 37.0 37.0 37.0 50-54 35.977850000000004 37.0 37.0 37.0 37.0 37.0 55-59 35.94575 37.0 37.0 37.0 37.0 37.0 60-64 35.97215 37.0 37.0 37.0 37.0 37.0 65-69 35.859 37.0 37.0 37.0 37.0 37.0 70-74 35.85855 37.0 37.0 37.0 37.0 37.0 75-79 35.89805 37.0 37.0 37.0 37.0 37.0 80-84 35.80290000000001 37.0 37.0 37.0 37.0 37.0 85-89 35.773250000000004 37.0 37.0 37.0 37.0 37.0 90-94 35.7355 37.0 37.0 37.0 37.0 37.0 95-99 35.785250000000005 37.0 37.0 37.0 37.0 37.0 100-104 35.69155 37.0 37.0 37.0 37.0 37.0 105-109 35.75305 37.0 37.0 37.0 37.0 37.0 110-114 35.63035 37.0 37.0 37.0 37.0 37.0 115-119 35.518550000000005 37.0 37.0 37.0 37.0 37.0 120-124 35.45085 37.0 37.0 37.0 37.0 37.0 125-129 35.499 37.0 37.0 37.0 37.0 37.0 130-134 35.326249999999995 37.0 37.0 37.0 32.2 37.0 135-139 35.3024 37.0 37.0 37.0 29.8 37.0 140-144 35.09615 37.0 37.0 37.0 29.8 37.0 145-149 34.912 37.0 37.0 37.0 27.4 37.0 150-151 34.5145 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 3.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 1.0 15 3.0 16 5.0 17 0.0 18 0.0 19 1.0 20 1.0 21 4.0 22 2.0 23 5.0 24 8.0 25 5.0 26 9.0 27 9.0 28 13.0 29 24.0 30 24.0 31 37.0 32 78.0 33 113.0 34 236.0 35 614.0 36 2618.0 37 185.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.07329317269076 22.08835341365462 12.073293172690763 28.76506024096386 2 28.875 23.5 30.925000000000004 16.7 3 20.875 27.675 32.475 18.975 4 23.375 33.275 24.75 18.6 5 24.65 37.0 21.05 17.299999999999997 6 21.075 40.825 22.825 15.275 7 21.349999999999998 23.0 38.275 17.375 8 21.325 27.975 27.025 23.674999999999997 9 21.325 25.025 30.45 23.200000000000003 10-14 23.93 29.595 25.91 20.565 15-19 24.22 27.35 27.029999999999998 21.4 20-24 22.268381800890936 28.40983032183793 27.874267981380452 21.447519895890686 25-29 23.013767209011263 28.035043804755944 27.5694618272841 21.381727158948685 30-34 22.341810172206646 28.344012815378456 27.913496195434522 21.400680816980376 35-39 22.89403874067771 28.890334851594172 26.958306221532606 21.257320186195507 40-44 22.54592781698954 27.9821795064324 28.062271612354206 21.409621064223856 45-49 22.179397337070778 28.075883471819 28.346180798878766 21.398538392231455 50-54 22.92907552930577 28.39981981080134 27.24360578607538 21.42749887381751 55-59 23.434292866082604 28.055068836045056 27.914893617021274 20.595744680851062 60-64 21.837929826317634 28.039441413484155 28.124530757295158 21.99809800290305 65-69 22.835985189632744 27.56429500650455 27.599319523666566 22.000400280196136 70-74 23.133917396745932 28.035043804755944 27.449311639549435 21.381727158948685 75-79 22.96871088861076 27.619524405506883 27.404255319148934 22.007509386733418 80-84 23.453453453453456 27.762762762762762 27.64764764764765 21.136136136136134 85-89 23.85481852315394 27.694618272841055 26.853566958698373 21.59699624530663 90-94 23.17048753628992 28.611472619881873 27.28000800880969 20.93803183501852 95-99 24.370589118574504 27.69407878272186 27.21857950848391 20.71675259021973 100-104 23.74468085106383 27.669586983729662 28.16520650813517 20.42052565707134 105-109 23.1639549436796 28.52565707133917 27.28911138923655 21.02127659574468 110-114 22.713849542019123 28.835277040892937 27.23359527503879 21.21727814204915 115-119 23.844806007509387 28.130162703379224 26.95369211514393 21.07133917396746 120-124 25.08635794743429 27.939924906132667 26.382978723404253 20.59073842302879 125-129 24.419303163796556 27.733279935923104 27.102523027633158 20.74489387264718 130-134 24.227707404996746 27.041506033144746 28.63365543483703 20.097131127021477 135-139 25.483128066486433 27.250425553219188 27.02012616401322 20.246320216281166 140-144 25.691976575404173 28.394814555283048 26.30762300415436 19.605585865158417 145-149 26.904976469410236 27.505757484730147 26.334234504856312 19.255031541003305 150-151 26.4521782674011 28.84326489734602 26.139208813219827 18.56534802203305 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 1.5 9 1.0 10 0.5 11 0.5 12 0.0 13 0.5 14 0.5 15 0.0 16 1.0 17 1.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 2.5 24 4.0 25 7.0 26 10.0 27 7.0 28 7.0 29 8.5 30 15.0 31 27.0 32 30.0 33 36.0 34 47.5 35 59.0 36 70.0 37 86.0 38 137.5 39 177.0 40 192.0 41 204.5 42 219.5 43 257.5 44 301.0 45 298.0 46 257.5 47 234.0 48 225.5 49 217.0 50 169.5 51 126.5 52 113.0 53 94.5 54 83.0 55 76.5 56 60.5 57 39.0 58 22.0 59 16.5 60 17.0 61 13.0 62 9.5 63 4.5 64 1.5 65 2.0 66 1.0 67 0.0 68 0.0 69 1.0 70 1.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.105 25-29 0.125 30-34 0.12 35-39 0.105 40-44 0.11499999999999999 45-49 0.11 50-54 0.105 55-59 0.125 60-64 0.105 65-69 0.06999999999999999 70-74 0.125 75-79 0.125 80-84 0.1 85-89 0.125 90-94 0.11 95-99 0.105 100-104 0.125 105-109 0.125 110-114 0.105 115-119 0.125 120-124 0.125 125-129 0.12 130-134 0.135 135-139 0.13 140-144 0.105 145-149 0.13 150-151 0.15 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.925 #Duplication Level Percentage of deduplicated Percentage of total 1 71.01232723197609 47.525 2 17.146059021292494 22.95 3 7.022786701531565 14.099999999999998 4 2.577512140455734 6.9 5 1.419499439671274 4.75 6 0.44826298094882333 1.7999999999999998 7 0.0747104968248039 0.35000000000000003 8 0.2614867388868136 1.4000000000000001 9 0.03735524841240195 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGCTATGAATACAAGTACATTGTTGATGGTGAGTGGACCATCAACAAACA 9 0.22499999999999998 No Hit AGAACACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATC 8 0.2 No Hit GCTAGAAGTGACTCTACCCTTTGCATTACTTTTTCAATCAATCACTGCTG 8 0.2 No Hit AAATGGAAGTAAGGGAAAAATTCCAGGAAATAAGTAAAGAGGCTGCCAAA 8 0.2 No Hit ACAGCCTCTTGTTCAAGCAAAGCTAGGCAATTAAAGACCTTCATCGCAGC 8 0.2 No Hit CTATACATGTGGTTTGTTGGGTCTAAATAGTGTCAAACATAACTTGATGC 8 0.2 No Hit ACATGATGCCGGGTATGGTGCAGGTGGTGGGAAGGGTGAGGGTGGCGGCC 8 0.2 No Hit CTTCGATTAACAGCTGGTGTCTCACCTCTGTCTCTGCCTCTAAGAGATCA 8 0.2 No Hit GTTCAATGCATGCAGGTGTGGCCTCCAACTGGATTGAAGAAGTTCGAGAC 7 0.17500000000000002 No Hit GCTGGAGAACTGATGATGAGGTTATGGCCATGTACGAGGAGTGGCTTGTA 7 0.17500000000000002 No Hit CGGAACTCGGGTAGAAAGGGGAACAAAAATCAAACAACGAAGAAAGAAAG 6 0.15 No Hit GCAATGGTGATTATTAACCCAGGCAACCCCACTGGTCAGTGTCTTAGTGA 6 0.15 No Hit GAAGATGAAGCTGCCCCCTACTTTGCTAGTAATTTCCTACTCTTTGCAAT 6 0.15 No Hit CACAGAGTGGTCGTGTAGGAGCAGTGACAATCGCTACAAATATGGCAGGT 6 0.15 No Hit GTGGAATTGGATAACTACCTCCGTACAACTGGCATTACTGGGATAGTAAA 6 0.15 No Hit AGCAGTTCGACCAATGCTGAGGGTGCTCAAAATAATCTTAAGGTTGATTT 6 0.15 No Hit TGAGTGAGTACATGGAAAGACACACTGTTGCTAAGCTGATTGGTTCGCCT 6 0.15 No Hit GCTTGTAAGAGATGGCTTCCTCCTCTATGATCTCATCGGCAGCCGTTGCC 6 0.15 No Hit GTGGACCACATCAGGAAGGATGAAGATGCCATTTTGCACGAAGAAAGAAG 6 0.15 No Hit GGTCAATGTCTTTTGTTGGAACCCACGAGTATTTGGCACCTGAGATCATC 6 0.15 No Hit GGTGAACTAAAAAGTAAAGAAAAGAAAGGTAGACCCACGCGAACTCGGTG 6 0.15 No Hit AGTGGACATACTGGATGCAGTTGACATCCTTGCTTTATTCATAGCTCGTG 6 0.15 No Hit CGCAAGGAAGGAGATTCTCCGGCCGGTTATGGCAGCGGCTTCGTCTCCTG 5 0.125 No Hit TTTTAGTGCAAAAGCTCTTGTATAATCTTTGATTCTAGTTACCGTATCTG 5 0.125 No Hit AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 5 0.125 No Hit GTCGTTTCCTTCCTTCTCACCCGGGGGTGACCGTATTGCTTACGTTGATT 5 0.125 No Hit GCAGCTTGAAGCATGTGCCGGGGTTTATCGAGAAAGCAGGGGAGTTAAAA 5 0.125 No Hit GCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGC 5 0.125 No Hit GCTGTTATAGGTATCCACCTCCATCATACTAGAACTAGATTTATTTATTT 5 0.125 No Hit ACTTTATGACCCATCCGAGTTCCATGTGGTTAACCCGATGAAAAGGACTC 5 0.125 No Hit CTCTAGAAGGCGTCTTCATAAACGTCTTCGTTCCTAATCTCCTTCTTCAA 5 0.125 No Hit CCTGAACAAGTTGCTGAGTACACACTCAAGCTCCTTCAGAGGAGAATCCC 5 0.125 No Hit AGCTCAAAGAGGCATTCAGAGTTTTTGACAAGGATCAGAATGGTTTCATC 5 0.125 No Hit GTGTAGAGTAGAGAGTTTTACAGTTCAACTAACTTTTTTTTAAGGATTTC 5 0.125 No Hit ATCAAAGTCAGATACCAAGAGCGCTAAGCTCTCTGTGAACAAGAAACCAA 5 0.125 No Hit AGAAAATCCACCAACTGGTCCTCGAGGCCAGATTTACACTCCTGAGTTTC 5 0.125 No Hit AGAGAGGGGCACAACTGGAAGGATTAGAACTTGCCGCATGCATGATCTTA 5 0.125 No Hit CCACGTTCAATCCTAAGGTTTTCGAGCCTGTTGAGGGTTCCAAAAATCTT 5 0.125 No Hit GTTCAGTTCTCTTCAGGGCTAGCAAGGATGGAGAGAAGAGAAAATGGGAA 5 0.125 No Hit CTGCCAATATCAGAGATATCAATGATCAACCACCCAACCCTTACCAGCAA 5 0.125 No Hit AGTGGATGAGATGTTGACTTTGAGTAGCAAAAATGTACAAATTGTTGATA 5 0.125 No Hit GCAGTCTTCCAAGTCCATTAACTAGAAAGAAATTTACCATCGGGCTAATG 5 0.125 No Hit CTTCAATCGAGCGCTCGTTCTCCTGCTTTCTATCGCGACCAGCACATAAA 5 0.125 No Hit ATTTCTTCAACTCTTCTTCTTCTTTTTGACTCGCATCCCAAAATCTCTCC 5 0.125 No Hit CTCGAGAAGAAAGCAGAAAAGAAATTGAACGGTTGGGGTATTTTCGGTTC 5 0.125 No Hit GGAATTCCTGTTCTTGCTAAGCTAGATCACCAAAAAGTACTAAAATCCTA 5 0.125 No Hit GTTTCAGTTTATCTTCCCATGTTTCCTGTCCAGGTCCTATTTTAAATTTC 5 0.125 No Hit GCCTACAGAGAGTTTGTCAACGGTGATCTTGTTGTCAAGGATGTTTAGAT 5 0.125 No Hit CATGAGAGAAGGTATAGCTTAACTGAGAAACCATGAATACTCTAGTTTGT 5 0.125 No Hit CAGCTTTCCCAGTCAGTACCAGAAAGGCTAATGACATTACTTCCATTGCA 5 0.125 No Hit GAGCTGACTTAGATTGGATGAGGGTGCTATAGCGTAATTAAGATGATCAC 5 0.125 No Hit CAAGTTCAAGAGGGTGTGTGTGTTTTGTGGAAGCAACTCTGGCAACAGAC 5 0.125 No Hit CCGCAACCCTCTTGACCTCCCCTGGAAGGACTTGGAGATCGATCTGGTGA 5 0.125 No Hit GTCACTGTGGAGGCAGTTCAAAGCAGAGACCGAGTATAGTGTGACCCAAG 5 0.125 No Hit GGAGAGTATGATCGGCACGGGTTCCAAAAGATGCTTGACAATTTTCAAGC 5 0.125 No Hit CAAGATTCTCTCCTCCTTTCTTTCCCTTTTCCCAAGAAACCAATCAGAAA 5 0.125 No Hit GTTTTGTTTATTTAGATATAGCTTGTCAATCCATTACTGCGATTTTGTGC 5 0.125 No Hit TGTTACTCCAAAACGAGTGGTTGAGATAGTTGAGATGCTAAGAAAGGGGG 5 0.125 No Hit TGGATGGCCATTTGAGCCTTTCCATGTTCCAGAGGATGTTAAGCAGCACT 5 0.125 No Hit GGAAACAATTTGTGGAAGCGCAAAGCTGATGCTGCTTTCCAGATGAAGAA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0125 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.05 0.0 0.0 0.0 0.0 82-83 0.2125 0.0 0.0 0.0 0.0 84-85 0.2625 0.0 0.0 0.0 0.0 86-87 0.375 0.0 0.0 0.0 0.0 88-89 0.45 0.0 0.0 0.0 0.0 90-91 0.525 0.0 0.0 0.0 0.0 92-93 0.5875 0.0 0.0 0.0 0.0 94-95 0.75 0.0 0.0 0.0 0.0 96-97 0.8375 0.0 0.0 0.0 0.0 98-99 1.1124999999999998 0.0 0.0 0.0 0.0 100-101 1.35 0.0 0.0 0.0 0.0 102-103 1.7875 0.0 0.0 0.0 0.0 104-105 2.1375 0.0 0.0 0.0 0.0 106-107 2.4875 0.0 0.0 0.0 0.0 108-109 2.775 0.0 0.0 0.0 0.0 110-111 3.0375 0.0 0.0 0.0 0.0 112-113 3.425 0.0 0.0 0.0 0.0 114-115 3.7 0.0 0.0 0.0 0.0 116-117 4.275 0.0 0.0 0.0 0.0 118-119 5.0375 0.0 0.0 0.0 0.0 120-121 5.262499999999999 0.0 0.0 0.0 0.0 122-123 5.7 0.0 0.0 0.0 0.0 124-125 6.4 0.0 0.0 0.0 0.0 126-127 7.0 0.0 0.0 0.0 0.0 128-129 7.6625 0.0 0.0 0.0 0.0 130-131 8.0625 0.0 0.0 0.0 0.0 132-133 8.525 0.0 0.0 0.0 0.0 134-135 9.1125 0.0 0.0 0.0 0.0 136-137 9.7 0.0 0.0 0.0 0.0 138-139 10.325 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTTTTT 35 0.0035136614 20.735985 100-104 >>END_MODULE Read 1016919 spots for SRR13695463.sra Written 1016919 spots for SRR13695463.sra Read 1016919 spots for SRR13695463.sra Written 1016919 spots for SRR13695463.sra Read 1016919 spots for SRR13695463.sra Written 1016919 spots for SRR13695463.sra Read 1016919 spots for SRR13695463.sra Written 1016919 spots for SRR13695463.sra Read 1016919 spots for SRR13695463.sra Written 1016919 spots for SRR13695463.sra Read 1016919 spots for SRR13695463.sra Written 1016919 spots for SRR13695463.sra Read 1016919 spots for SRR13695463.sra Written 1016919 spots for SRR13695463.sra Read 1016919 spots for SRR13695463.sra Written 1016919 spots for SRR13695463.sra Read 1016919 spots for SRR13695463.sra Written 1016919 spots for SRR13695463.sra Read 1016919 spots for SRR13695463.sra Written 1016919 spots for SRR13695463.sra Read 1016919 spots for SRR13695463.sra Written 1016919 spots for SRR13695463.sra Read 1016919 spots for SRR13695463.sra Written 1016919 spots for SRR13695463.sra Read 1016919 spots for SRR13695463.sra Written 1016919 spots for SRR13695463.sra Read 1016919 spots for SRR13695463.sra Written 1016919 spots for SRR13695463.sra Read 1016919 spots for SRR13695463.sra Written 1016919 spots for SRR13695463.sra Read 1016919 spots for SRR13695463.sra Written 1016919 spots for SRR13695463.sra Read 1016924 spots for SRR13695463.sra Written 1016924 spots for SRR13695463.sra Read 1016919 spots for SRR13695463.sra Written 1016919 spots for SRR13695463.sra Read 1016919 spots for SRR13695463.sra Written 1016919 spots for SRR13695463.sra Read 1016919 spots for SRR13695463.sra Written 1016919 spots for SRR13695463.sra SRR ids: ['SRR13695463.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_f50t7evn SRR13695463.sra spots: 20338385 blocks: [[1, 1016919], [1016920, 2033838], [2033839, 3050757], [3050758, 4067676], [4067677, 5084595], [5084596, 6101514], [6101515, 7118433], [7118434, 8135352], [8135353, 9152271], [9152272, 10169190], [10169191, 11186109], [11186110, 12203028], [12203029, 13219947], [13219948, 14236866], [14236867, 15253785], [15253786, 16270704], [16270705, 17287623], [17287624, 18304542], [18304543, 19321461], [19321462, 20338385]] SRR13695463 file size 6890172 SRR13695463 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695463 SRR13695463_1.fastq SRR13695463_2.fastq Input file: SRR13695463_1.fastq Paired file: SRR13695463_2.fastq trimmed: SRR13695463-trimmed-pair1.fastq, SRR13695463-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 05:04:27 2025 >> started Wed Feb 12 05:04:51 2025 >> done (23.816s) 20338385 read pairs processed; of these: 147 ( 0.00%) short read pairs filtered out after trimming by size control 827 ( 0.00%) empty read pairs filtered out after trimming by size control 20337411 (100.00%) read pairs available; of these: 2729625 (13.42%) trimmed read pairs available after processing 17607786 (86.58%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 3 0.00% 19 4 0.00% 20 0 0.00% 21 0 0.00% 22 2 0.00% 23 1 0.00% 24 2 0.00% 25 4 0.00% 26 4 0.00% 27 2 0.00% 28 9 0.00% 29 2 0.00% 30 2 0.00% 31 5 0.00% 32 4 0.00% 33 5 0.00% 34 7 0.00% 35 8 0.00% 36 4 0.00% 37 5 0.00% 38 6 0.00% 39 18 0.00% 40 9 0.00% 41 17 0.00% 42 20 0.00% 43 6 0.00% 44 16 0.00% 45 20 0.00% 46 35 0.00% 47 29 0.00% 48 32 0.00% 49 39 0.00% 50 73 0.00% 51 45 0.00% 52 57 0.00% 53 67 0.00% 54 96 0.00% 55 90 0.00% 56 112 0.00% 57 138 0.00% 58 159 0.00% 59 189 0.00% 60 231 0.00% 61 269 0.00% 62 334 0.00% 63 363 0.00% 64 435 0.00% 65 471 0.00% 66 546 0.00% 67 610 0.00% 68 742 0.00% 69 883 0.00% 70 953 0.00% 71 1211 0.01% 72 1510 0.01% 73 1597 0.01% 74 1819 0.01% 75 2003 0.01% 76 2334 0.01% 77 2576 0.01% 78 2945 0.01% 79 3328 0.02% 80 3989 0.02% 81 4439 0.02% 82 4900 0.02% 83 5559 0.03% 84 6535 0.03% 85 7110 0.03% 86 7438 0.04% 87 8160 0.04% 88 8938 0.04% 89 9215 0.05% 90 10683 0.05% 91 10780 0.05% 92 12158 0.06% 93 13482 0.07% 94 14533 0.07% 95 15944 0.08% 96 16293 0.08% 97 17590 0.09% 98 18653 0.09% 99 19297 0.09% 100 20286 0.10% 101 20677 0.10% 102 22685 0.11% 103 23520 0.12% 104 25491 0.13% 105 26361 0.13% 106 27241 0.13% 107 28892 0.14% 108 29281 0.14% 109 30358 0.15% 110 30728 0.15% 111 32245 0.16% 112 34033 0.17% 113 34710 0.17% 114 36004 0.18% 115 37474 0.18% 116 39344 0.19% 117 41105 0.20% 118 41543 0.20% 119 42141 0.21% 120 43873 0.22% 121 44643 0.22% 122 45765 0.23% 123 46836 0.23% 124 48357 0.24% 125 49775 0.24% 126 51959 0.26% 127 52716 0.26% 128 54159 0.27% 129 55054 0.27% 130 55881 0.27% 131 55601 0.27% 132 56627 0.28% 133 58409 0.29% 134 58818 0.29% 135 60609 0.30% 136 61531 0.30% 137 62708 0.31% 138 63215 0.31% 139 65777 0.32% 140 65686 0.32% 141 66628 0.33% 142 67597 0.33% 143 67532 0.33% 144 68900 0.34% 145 69708 0.34% 146 70571 0.35% 147 71722 0.35% 148 73686 0.36% 149 73856 0.36% 150 75105 0.37% 151 17607786 86.58% 20337411 reads passed initial QC criterion=sequence-density sequence-density=0.55 sequence-density-rank=1 fanout-score=2.16 fanout-score-rank=13 prefix-density=0.57 prefix-fanout=2.1 sequence=TGAGATGCCTCAGTGCATCCAAACATGGGTAG criterion=fanout-score sequence-density=0.01 sequence-density-rank=22 fanout-score=319.79 fanout-score-rank=1 prefix-density=0.27 prefix-fanout=16.4 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTC criterion=sequence-density sequence-density=0.74 sequence-density-rank=1 fanout-score=2.07 fanout-score-rank=23 prefix-density=0.74 prefix-fanout=2.1 sequence=CCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAATCATCATGTCTAGCACCTGCGACACCTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGAC criterion=fanout-score sequence-density=0.01 sequence-density-rank=27 fanout-score=23.96 fanout-score-rank=1 prefix-density=0.09 prefix-fanout=2.0 sequence=CCACCACTAAACCCAAAGCTCAAAAAAAATCTTAACTGCACCCGCTCATCCAGCAATGGCAGCAGCAACAATGGCCCTCTCCTCCCCTTCGCTAGCCGGAAAGGCGGTGAAGCTCAACCCCTCCTCCTCTGAGATCATGGGCAATGGCCGTGTCTCCATGAGGAAAACCACCAAGCCTGTTCCCTCCGGGAGCCCATGGTACGGACCAGACCGTGTTAAATACTTGGGCCCGTTCTCTGGTGAGCCCCCATCCTACTTGACTGGTGAGTTCCCTGGTGACTACGGCTGGGACACTGCTGG SRR13695463 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 05:05:28 Started mapping on | Feb 12 05:05:28 Finished on | Feb 12 05:07:28 Mapping speed, Million of reads per hour | 610.12 Number of input reads | 20337411 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 19201947 Uniquely mapped reads % | 94.42% Average mapped length | 293.99 Number of splices: Total | 18514258 Number of splices: Annotated (sjdb) | 18138694 Number of splices: GT/AG | 18127860 Number of splices: GC/AG | 309217 Number of splices: AT/AC | 10586 Number of splices: Non-canonical | 66595 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.03% Deletion average length | 2.85 Insertion rate per base | 0.02% Insertion average length | 2.32 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 497322 % of reads mapped to multiple loci | 2.45% Number of reads mapped to too many loci | 79696 % of reads mapped to too many loci | 0.39% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.63% % of reads unmapped: other | 0.11% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 638409 638409 638409 N_multimapping 497322 497322 497322 N_noFeature 696426 18709083 984759 N_ambiguous 322009 1790 116144 UnstrandedReadsAssigned:18183512 PositiveStrandReadsAssigned:491074 NegativeStrandReadsAssigned:18101044 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695463 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695463-trimmed-pair1.fastq SRR13695463-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 20,337,411 reads, 18,216,698 reads pseudoaligned [quant] estimated average fragment length: 239.568 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,044 rounds 52401 SRR13695463.ke.tsv 34699 SRR13695463.se.tsv 87100 total ==> SRR13695463.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1779.43 475 12.921 Potri.005G024800.1.v4.1 1035 796.432 198 12.0337 Potri.004G059700.1.v4.1 961 722.507 11 0.736942 Potri.007G009000.2.v4.1 1416 1177.43 0 0 Potri.003G141000.2.v4.1 2943 2704.43 964.432 17.2615 Potri.016G087400.1.v4.1 270 88.1542 744 408.519 Potri.015G069301.1.v4.1 564 332.915 0 0 Potri.010G195200.1.v4.1 1773 1534.43 48 1.51418 Potri.012G127500.1.v4.1 977 738.47 205 13.437 ==> SRR13695463.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 548 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 318 Potri.001G212900.v4.1 6 Potri.001G182400.v4.1 3 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 3 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR13695463 completed mapping pipeline successfully