Starting /dee2/code/volunteer_pipeline.sh SRR13695465 current disk space = 3048986505216 free memory = 1437533492 SRR13695465 SRAfilesize 1a9daaba72a954c9dc52ebf7b61fa492 SRR13695465.sra SRR13695465.sra file validated SRR13695465 is paired end SRR13695465 is conventional basespace SRR13695465 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695465_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.568 37.0 37.0 37.0 37.0 37.0 2 36.307 37.0 37.0 37.0 37.0 37.0 3 36.6025 37.0 37.0 37.0 37.0 37.0 4 36.541 37.0 37.0 37.0 37.0 37.0 5 36.5895 37.0 37.0 37.0 37.0 37.0 6 36.587 37.0 37.0 37.0 37.0 37.0 7 36.558 37.0 37.0 37.0 37.0 37.0 8 36.601 37.0 37.0 37.0 37.0 37.0 9 36.6105 37.0 37.0 37.0 37.0 37.0 10-14 36.5783 37.0 37.0 37.0 37.0 37.0 15-19 36.5523 37.0 37.0 37.0 37.0 37.0 20-24 36.552200000000006 37.0 37.0 37.0 37.0 37.0 25-29 36.4499 37.0 37.0 37.0 37.0 37.0 30-34 36.410900000000005 37.0 37.0 37.0 37.0 37.0 35-39 36.4002 37.0 37.0 37.0 37.0 37.0 40-44 36.3996 37.0 37.0 37.0 37.0 37.0 45-49 36.3566 37.0 37.0 37.0 37.0 37.0 50-54 36.359300000000005 37.0 37.0 37.0 37.0 37.0 55-59 36.3395 37.0 37.0 37.0 37.0 37.0 60-64 36.373599999999996 37.0 37.0 37.0 37.0 37.0 65-69 36.2995 37.0 37.0 37.0 37.0 37.0 70-74 36.311 37.0 37.0 37.0 37.0 37.0 75-79 36.3056 37.0 37.0 37.0 37.0 37.0 80-84 36.1675 37.0 37.0 37.0 37.0 37.0 85-89 36.1981 37.0 37.0 37.0 37.0 37.0 90-94 36.1461 37.0 37.0 37.0 37.0 37.0 95-99 36.0638 37.0 37.0 37.0 37.0 37.0 100-104 36.0758 37.0 37.0 37.0 37.0 37.0 105-109 36.0775 37.0 37.0 37.0 37.0 37.0 110-114 36.0595 37.0 37.0 37.0 37.0 37.0 115-119 36.055899999999994 37.0 37.0 37.0 37.0 37.0 120-124 35.981700000000004 37.0 37.0 37.0 37.0 37.0 125-129 35.9468 37.0 37.0 37.0 37.0 37.0 130-134 35.8937 37.0 37.0 37.0 37.0 37.0 135-139 35.7744 37.0 37.0 37.0 37.0 37.0 140-144 35.5847 37.0 37.0 37.0 37.0 37.0 145-149 35.3082 37.0 37.0 37.0 34.6 37.0 150-151 35.09725 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 0.0 22 1.0 23 0.0 24 1.0 25 2.0 26 3.0 27 18.0 28 15.0 29 22.0 30 24.0 31 34.0 32 48.0 33 93.0 34 134.0 35 333.0 36 2946.0 37 325.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 30.975 10.375 12.049999999999999 46.6 2 21.001006036217305 14.43661971830986 36.896378269617706 27.66599597585513 3 20.0 17.1 24.224999999999998 38.675 4 22.425 25.275 24.175 28.125 5 22.925 30.875000000000004 25.2 21.0 6 19.35 35.15 24.7 20.8 7 15.174999999999999 26.5 41.975 16.35 8 17.275 27.800000000000004 31.075000000000003 23.849999999999998 9 17.275 25.05 33.2 24.474999999999998 10-14 18.529999999999998 29.65 27.839999999999996 23.98 15-19 19.695 28.38 28.175 23.75 20-24 19.564999999999998 28.549999999999997 27.994999999999997 23.89 25-29 19.375 27.884999999999998 29.18 23.56 30-34 19.68 29.325000000000003 27.325 23.669999999999998 35-39 19.77 27.98 27.994999999999997 24.255 40-44 19.045 28.904999999999998 28.810000000000002 23.24 45-49 19.23 29.160000000000004 27.85 23.76 50-54 20.195 28.34 28.015 23.45 55-59 19.695 28.084999999999997 28.34 23.880000000000003 60-64 19.81 28.439999999999998 28.23 23.52 65-69 19.48 28.615000000000002 28.53 23.375 70-74 19.99 28.505000000000003 28.01 23.494999999999997 75-79 19.615 28.360000000000003 28.49 23.535 80-84 20.135 27.76 27.55 24.555 85-89 20.855 29.2 27.139999999999997 22.805 90-94 20.990000000000002 28.315 27.18 23.515 95-99 19.855 28.54 27.750000000000004 23.855 100-104 20.635 28.73 27.544999999999998 23.09 105-109 20.424999999999997 29.244999999999997 26.655 23.674999999999997 110-114 20.155 28.884999999999998 27.665 23.294999999999998 115-119 21.01 28.860000000000003 26.619999999999997 23.51 120-124 20.49 29.04 26.779999999999998 23.69 125-129 21.22 28.139999999999997 27.615000000000002 23.025000000000002 130-134 20.849999999999998 28.544999999999998 25.95 24.654999999999998 135-139 22.165000000000003 28.32 26.145000000000003 23.369999999999997 140-144 20.93 28.26 26.825 23.985 145-149 20.41 28.54 26.57 24.48 150-151 20.95 28.925 25.637500000000003 24.4875 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.0 20 0.5 21 0.5 22 2.0 23 2.5 24 6.0 25 6.5 26 4.0 27 4.0 28 12.0 29 20.0 30 21.5 31 24.5 32 26.5 33 42.0 34 53.5 35 62.5 36 97.5 37 121.5 38 135.0 39 176.0 40 200.5 41 227.5 42 247.0 43 256.0 44 269.5 45 280.0 46 275.5 47 253.0 48 224.0 49 179.0 50 158.5 51 141.5 52 120.0 53 87.0 54 57.5 55 43.5 56 45.0 57 37.5 58 17.5 59 16.0 60 16.5 61 12.5 62 4.5 63 1.5 64 2.5 65 2.5 66 1.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.0 72 1.0 73 1.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.6 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.95 #Duplication Level Percentage of deduplicated Percentage of total 1 72.79774489076814 51.65 2 18.217054263565892 25.85 3 6.025369978858351 12.825000000000001 4 1.8322762508809023 5.2 5 0.5637773079633545 2.0 6 0.4580690627202256 1.95 7 0.10570824524312897 0.525 8 0.0 0.0 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CAGTCAACAAGATTCGAGGGATTAAATTATAGAGTTTTCAGAAAAAAGAG 7 0.17500000000000002 No Hit CACAATTTCAATTTTCCGAAGGACCTGCTCCAAGGATTTATTACCATCTC 7 0.17500000000000002 No Hit GTTGTTGTGTCTGCAATGTAAGCATTAGGCTTCTTCTTATCCTTCCTCAA 7 0.17500000000000002 No Hit GGCAACTTTTGTATCTGCTTCTTTTGCAACAACGGCAACTTCTTCTGCAG 6 0.15 No Hit CTCGTGTCCCTAGGCTCCATTCTCTCACAGCAACAGCAACGAGGAGTTCC 6 0.15 No Hit ACCACATCAACCTCCAAAGTTTTCCTCTCTCCAGTTCCACCTTTCTTCCC 6 0.15 No Hit GCATACAGTACAAACTACATCGTGTACGCCTCATCGTTTGATTGGAGTGT 6 0.15 No Hit GTGCATTATTTGTTGCTACTGCCGGCAGTGTCTTCATGTACTCAAATATC 6 0.15 No Hit CTCGCCTTCTCTCGGTATAAACACGGTCTCTCTGGCTGTTCAGAACTTCA 6 0.15 No Hit CCTGACCACTTCGTATTGTTCCTGTTGATTCCTCTGATGTTAGTCTTGTC 6 0.15 No Hit CTCCTCCTCCCAGGCATTGGCATTGTGGAATATGAAGCAATTTGGAAGCT 6 0.15 No Hit GACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTG 6 0.15 No Hit GGCAAAAGCTCATCAAATTCATCTGAGATGAAGACTCGTCTAACATACAG 6 0.15 No Hit CTCTTTTGGTGGCCCTTCATTGAGCACGTCAAATTCAGATGCTTGAGCAT 6 0.15 No Hit GAGTCAGAAAATCAGCAACATGGTTCACTTGCCCGTGATGAATGGGTGAG 6 0.15 No Hit GTGGCTGCAGATGTTATTACCAGCATTCCAAGAATCAGAGAGTAGTGAAA 6 0.15 No Hit TCTGAAAGTCCGAGTGGGTCGAATCCGAAGTCACCTGGAAGGCTATAGAG 5 0.125 No Hit ATTAGATCTAGAAATCTCCAATAAGCTACCATCCTTTGACAGCAATTCAA 5 0.125 No Hit TATCGATAAAATATCAACAGCCATCTTGGAAAGTGTTGGATACTTCAGCC 5 0.125 No Hit ATCCAATCACCTTCTGAATCTGGACAACTTGGGAAAGTTTATAGCCAAAA 5 0.125 No Hit CCCCATTCAAAACGACCTGTCGTTCCTCTTCAACAATGCAGGATCCGTAA 5 0.125 No Hit GTGCAAAGCGATTAGAATATTCAATCTTGTCCTCTGGCATTGGCCTGTGG 5 0.125 No Hit CGGGAACATTGTCAGTCTTGAAAATTCCAATGCAGACACCAATCCTATCT 5 0.125 No Hit CTCCTAGCAAAGTGGATCCAACAATCCCAACATTCCCAATCAACAGCTTC 5 0.125 No Hit CTCGAAGAAGGCCATAAGGAACTGCTCCAAAGTTTCTTGAATCCTTGCTG 5 0.125 No Hit CTTCGAAGGAGTCTGCTGCTCGTTTTGTGGAGATCGGAATCCTTGTGCAG 5 0.125 No Hit AGAGAAACTAGTTACGTTGTTGTGCATAGTGGGGTGTGATTCCTTCTCTT 5 0.125 No Hit GGACATCAATCCTGTTAAGTAGTTCTGTATAGGAGGAAGTCAGTGAAAGT 5 0.125 No Hit CTGAAATATAATAGCTGGTTTGAAAAGAAGCCACAATATCCACCTGGTTC 5 0.125 No Hit CTGAATTTCAGCTCGACAGCTTCTTTCTTCGACTTCTTTGGATTTGGTGA 5 0.125 No Hit ATTCCTGCAACGCCCAACATGGCCCACCGACCATTCACAAGCTCAGCCTG 5 0.125 No Hit CCGACGACCAAAATGTTTATGTGATCCATTATCTAAAGAAGAATGATTAC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0125 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.1125 0.0 0.0 0.0 0.0 80-81 0.2375 0.0 0.0 0.0 0.0 82-83 0.275 0.0 0.0 0.0 0.0 84-85 0.3 0.0 0.0 0.0 0.0 86-87 0.42500000000000004 0.0 0.0 0.0 0.0 88-89 0.55 0.0 0.0 0.0 0.0 90-91 0.7375 0.0 0.0 0.0 0.0 92-93 1.1124999999999998 0.0 0.0 0.0 0.0 94-95 1.375 0.0 0.0 0.0 0.0 96-97 1.5625 0.0 0.0 0.0 0.0 98-99 1.8125 0.0 0.0 0.0 0.0 100-101 2.1125 0.0 0.0 0.0 0.0 102-103 2.2875 0.0 0.0 0.0 0.0 104-105 2.5125 0.0 0.0 0.0 0.0 106-107 3.1125 0.0 0.0 0.0 0.0 108-109 3.55 0.0 0.0 0.0 0.0 110-111 4.050000000000001 0.0 0.0 0.0 0.0 112-113 4.475 0.0 0.0 0.0 0.0 114-115 4.6625 0.0 0.0 0.0 0.0 116-117 5.225 0.0 0.0 0.0 0.0 118-119 5.5875 0.0 0.0 0.0 0.0 120-121 5.875 0.0 0.0 0.0 0.0 122-123 6.4 0.0 0.0 0.0 0.0 124-125 6.7 0.0 0.0 0.0 0.0 126-127 7.4 0.0 0.0 0.0 0.0 128-129 8.1875 0.0 0.0 0.0 0.0 130-131 8.587499999999999 0.0 0.0 0.0 0.0 132-133 9.125 0.0 0.0 0.0 0.0 134-135 9.6375 0.0 0.0 0.0 0.0 136-137 9.962499999999999 0.0 0.0 0.0 0.0 138-139 10.4875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAAAAAA 30 0.0014437955 24.166668 45-49 >>END_MODULE SRR13695465 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695465_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.23475 37.0 37.0 37.0 37.0 37.0 2 36.2195 37.0 37.0 37.0 37.0 37.0 3 36.2645 37.0 37.0 37.0 37.0 37.0 4 36.2725 37.0 37.0 37.0 37.0 37.0 5 36.2395 37.0 37.0 37.0 37.0 37.0 6 36.3225 37.0 37.0 37.0 37.0 37.0 7 36.293 37.0 37.0 37.0 37.0 37.0 8 36.3385 37.0 37.0 37.0 37.0 37.0 9 36.327 37.0 37.0 37.0 37.0 37.0 10-14 36.3857 37.0 37.0 37.0 37.0 37.0 15-19 36.347699999999996 37.0 37.0 37.0 37.0 37.0 20-24 36.28335 37.0 37.0 37.0 37.0 37.0 25-29 36.28255 37.0 37.0 37.0 37.0 37.0 30-34 36.207049999999995 37.0 37.0 37.0 37.0 37.0 35-39 36.198150000000005 37.0 37.0 37.0 37.0 37.0 40-44 36.18255 37.0 37.0 37.0 37.0 37.0 45-49 36.1454 37.0 37.0 37.0 37.0 37.0 50-54 36.16185 37.0 37.0 37.0 37.0 37.0 55-59 36.06255 37.0 37.0 37.0 37.0 37.0 60-64 36.07755 37.0 37.0 37.0 37.0 37.0 65-69 36.04305000000001 37.0 37.0 37.0 37.0 37.0 70-74 35.996449999999996 37.0 37.0 37.0 37.0 37.0 75-79 35.9708 37.0 37.0 37.0 37.0 37.0 80-84 35.964299999999994 37.0 37.0 37.0 37.0 37.0 85-89 35.88915000000001 37.0 37.0 37.0 37.0 37.0 90-94 35.842 37.0 37.0 37.0 37.0 37.0 95-99 35.82305 37.0 37.0 37.0 37.0 37.0 100-104 35.82505 37.0 37.0 37.0 37.0 37.0 105-109 35.87135 37.0 37.0 37.0 37.0 37.0 110-114 35.723349999999996 37.0 37.0 37.0 37.0 37.0 115-119 35.694050000000004 37.0 37.0 37.0 37.0 37.0 120-124 35.62025 37.0 37.0 37.0 37.0 37.0 125-129 35.5683 37.0 37.0 37.0 37.0 37.0 130-134 35.47475 37.0 37.0 37.0 37.0 37.0 135-139 35.382400000000004 37.0 37.0 37.0 32.2 37.0 140-144 35.261250000000004 37.0 37.0 37.0 34.6 37.0 145-149 35.1419 37.0 37.0 37.0 27.4 37.0 150-151 34.79275 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 0.0 15 1.0 16 0.0 17 1.0 18 1.0 19 2.0 20 2.0 21 2.0 22 2.0 23 3.0 24 3.0 25 5.0 26 6.0 27 11.0 28 15.0 29 16.0 30 24.0 31 39.0 32 64.0 33 108.0 34 209.0 35 598.0 36 2672.0 37 213.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 31.63700175482577 20.055151667084484 15.818500877412886 32.489345700676864 2 29.45 22.825 30.15 17.575 3 21.099999999999998 28.125 30.025000000000002 20.75 4 24.45 33.1 22.75 19.7 5 25.0 36.225 22.525000000000002 16.25 6 19.55 41.0 22.175 17.275 7 19.825 23.375 38.525 18.275 8 21.4 26.424999999999997 29.425 22.75 9 21.9 25.650000000000002 32.324999999999996 20.125 10-14 22.52 30.095 26.69 20.695 15-19 23.05 28.815 27.35 20.785 20-24 22.567412076642153 29.251088098454147 27.650207614187806 20.531292210715893 25-29 23.252439329497125 28.821616212159118 27.815861896422316 20.11008256192144 30-34 22.654725571621555 29.269024866163008 27.142642717766545 20.93360684444889 35-39 22.117164440442245 28.425634098754315 27.870328680774424 21.586872780029015 40-44 22.494621503977584 27.883124030619904 28.82373542802822 20.798519037374295 45-49 23.118871322793677 28.191915149089454 28.22193315989594 20.467280368220933 50-54 22.11216168892891 28.085446995847718 28.830856971334235 20.97153434388914 55-59 23.042281711283465 27.92594445834376 28.426319739804857 20.605454090567925 60-64 22.617439591775476 27.820301165641105 28.54069738356096 21.021561859022462 65-69 23.028059820937326 27.97979292752463 27.864752663432203 21.127394588105837 70-74 22.882161621215914 27.700775581686266 27.52064048036027 21.896422316737553 75-79 22.595817071950368 27.7944561192835 28.064645251676172 21.54508155708996 80-84 23.081540770385192 27.913956978489246 28.084042021010507 20.920460230115058 85-89 23.062296722541905 27.530647985989493 28.286214660995746 21.120840630472852 90-94 23.16890134080448 28.762257354412647 27.30638383029818 20.76245747448469 95-99 23.487918355095303 27.885336935314424 27.62019110510781 21.006553604482463 100-104 24.3832874655992 27.995996997748314 27.790843132349263 19.829872404303227 105-109 23.947960970728047 28.07605704278209 27.775831873905428 20.20015011258444 110-114 24.1682925609085 28.400620341187654 27.670218620241133 19.760868477662715 115-119 24.563422566925194 28.68651488616462 27.090317738303725 19.659744808606455 120-124 25.023767825869403 28.04603452589442 27.20040030022517 19.729797348011008 125-129 24.74732312618833 28.30481336935855 27.098969278494945 19.848894225958173 130-134 24.986238302557172 28.188960616524046 26.747735575238952 20.07706550567983 135-139 25.475380304243394 27.9273418734988 26.89151321056846 19.705764611689354 140-144 26.77972885086798 28.510680874480965 25.614087748261543 19.095502526389517 145-149 26.611289031224977 27.502001601281023 26.526220976781424 19.36048839071257 150-151 27.289789789789793 27.515015015015017 25.900900900900904 19.294294294294296 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.5 7 0.5 8 0.0 9 1.5 10 1.5 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.5 18 0.5 19 0.0 20 0.0 21 0.5 22 0.5 23 0.0 24 1.5 25 4.0 26 5.5 27 7.0 28 8.5 29 14.0 30 19.5 31 27.0 32 38.0 33 48.0 34 57.5 35 65.5 36 84.0 37 118.0 38 140.0 39 166.5 40 201.0 41 231.0 42 262.5 43 281.5 44 285.5 45 287.0 46 245.5 47 208.0 48 227.0 49 216.0 50 176.0 51 122.5 52 80.5 53 71.0 54 64.0 55 55.5 56 46.0 57 29.5 58 21.5 59 20.5 60 17.5 61 17.5 62 9.0 63 1.0 64 2.0 65 2.0 66 0.5 67 0.0 68 0.0 69 0.5 70 0.5 71 0.0 72 0.5 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 1.0 84 1.5 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27499999999999997 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.055 25-29 0.075 30-34 0.065 35-39 0.055 40-44 0.065 45-49 0.06 50-54 0.055 55-59 0.075 60-64 0.055 65-69 0.034999999999999996 70-74 0.075 75-79 0.06999999999999999 80-84 0.05 85-89 0.075 90-94 0.06 95-99 0.055 100-104 0.075 105-109 0.075 110-114 0.055 115-119 0.075 120-124 0.075 125-129 0.06999999999999999 130-134 0.08499999999999999 135-139 0.08 140-144 0.055 145-149 0.08 150-151 0.1 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.675 #Duplication Level Percentage of deduplicated Percentage of total 1 72.97488503714185 51.575 2 17.75733993632826 25.1 3 6.15493455960382 13.05 4 1.8040325433321542 5.1 5 0.7782101167315175 2.75 6 0.3537318712415989 1.5 7 0.14149274849663954 0.7000000000000001 8 0.0 0.0 9 0.035373187124159884 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CAGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 9 0.22499999999999998 No Hit GCCCTTGGTGTTGACATTCGGGAAGCAGCAACACGTATATTCTCTAATGC 7 0.17500000000000002 No Hit AGGGCCTCCTCACCCTTCGTCGAAAGGTTAGCTGCTGCTTTTTTCTCTGT 7 0.17500000000000002 No Hit GTTGATGGCAATGATAAATCTGGCGTTGACAATGACGAAATGTTTATTGA 7 0.17500000000000002 No Hit AGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATC 7 0.17500000000000002 No Hit GTTAAGGCTATATGGTTGAGAAATCCAAAGGAGGTGACAGAAGAAGAGTA 6 0.15 No Hit GAAACCTTACTTTCTTCGCACTCTCTTTCTCCTCTCCTCAACTCAAAACC 6 0.15 No Hit TGCTGATTTCGAAAGTGTGAGTAGTAAATGTGAATTCCTTAATTTCTGAT 6 0.15 No Hit TGATATTGGACGAACCTTGAAGCCTCACGAGTACATTGGCATGGTGAGGC 6 0.15 No Hit ACCAAGGCCAAAGACAGTAGTGACTTGAATACAGTTTCAGTATCATTTGA 6 0.15 No Hit TGGAGATCTGCAAACTATTGGCCTGTTGGATTATGAAAAAAGACTAAAAC 6 0.15 No Hit GTCCTAATCAAATAGCAGCTTCGAACATGAAATCGACTAAATGTTCTAAT 6 0.15 No Hit AGGTAACCTTCATTTTTCCATATCCATTGACATGTGCATTTAGTGATTCA 6 0.15 No Hit CCTGGAAGTTCCCGCTTCTTCCTAAGTCTTGAAGATAACCTCTTTAGGAT 6 0.15 No Hit GTTTGGTCATTGAAATGTTTTCTTTCATTTAAAAACTGCTCGTTGATTTA 6 0.15 No Hit CCGGAGAATTGGAAAGTGGAAACGCTGGTGAACCCGCAAAACTTATCAGG 5 0.125 No Hit ATCGACCAGTAATGCCAAACGGAATTTGACGAAGGAGTTCTGTGGCAGTC 5 0.125 No Hit GGAAGAGATCGGAGAGGGAAAGAACAAGAGACCAAGTCATCATAAAGGAA 5 0.125 No Hit CGAAGAATAACTTTTATTGAAAGACCGCGAGGGAGTCCGCGTCCGGAGCT 5 0.125 No Hit ATCCTGTTAAAAAAAGGTAAAGGTGTACGTTTGGCAACCTTAGTACTGCT 5 0.125 No Hit GTTTAGATATAGTTGAGAAACCAAGTAGTATGCCTATTGTAGATGCTGAG 5 0.125 No Hit TTTTTTTTCTCGATTTTGGATGTCGAAGTTTCTTTGTTAAAGTTACGGAT 5 0.125 No Hit GGCAGTTGTGGTTCGCATCGAAACAAAGCCTGTCTTACTTGGATGGCAGG 5 0.125 No Hit GTCAGGAAGAACACCGGAGATGAAAAATCAATTTGCTACCTTCGATGAGA 5 0.125 No Hit CGGGTAGAAAGGGGAACAAAAATCAAACAACGAAGAAAGAAAGAAAAGAA 5 0.125 No Hit GGATAGACCTGATCGTGGAGTGTGGACTCCTCTCCGCAGATCAGATGGAT 5 0.125 No Hit GGCAGCTCAAGCCTCTCTCTTTACTCCTCCAACCCTCTCCACCCTAAAAT 5 0.125 No Hit GTGCACATTCTTTTGGCAAAGACTTTACTGGAAACAGGAGCTTCAACCCC 5 0.125 No Hit CGCAGATATATAATATCTTCAGACCATACCGATTCTGAAGGAGTAGGTTT 5 0.125 No Hit GGGAATGAAGGGTGTTAGTCAAGTGCTTGTGGATAGAAAGTCAAACAAGG 5 0.125 No Hit GTTGACGGAGGCTGTTAGGCGTCGCCCTTACACTGTGGTACTCTTTGATG 5 0.125 No Hit CTGCGCTGTTTACTGCTGCAGATAAAGGTCATCTTGAGGTGGTTAAGGAA 5 0.125 No Hit ATTGGAGATGGGCTTTCAGATTTTGATATATACATCTCTGAGATTACAAG 5 0.125 No Hit TCCACCTTATGCATTCTTCTTTCCATCAAGCTACAGGATCTGTGCTGGGT 5 0.125 No Hit CGAAAGCCATTCTCTGAAAGAACATCAATATGGCTCCTAAACTTTCCTGT 5 0.125 No Hit CATACAAGTCGTATCAGACGGCGAGGATGATCTCCTTAACGCAGAGCATC 5 0.125 No Hit CAAGAAAAAGTATGACTAAAAGAGTTATAGGTGTCGAGGGTGATTCTGTT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0125 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.05 0.0 0.0 0.0 0.0 76-77 0.05 0.0 0.0 0.0 0.0 78-79 0.1125 0.0 0.0 0.0 0.0 80-81 0.2375 0.0 0.0 0.0 0.0 82-83 0.275 0.0 0.0 0.0 0.0 84-85 0.3 0.0 0.0 0.0 0.0 86-87 0.42500000000000004 0.0 0.0 0.0 0.0 88-89 0.55 0.0 0.0 0.0 0.0 90-91 0.7375 0.0 0.0 0.0 0.0 92-93 1.125 0.0 0.0 0.0 0.0 94-95 1.4 0.0 0.0 0.0 0.0 96-97 1.5875 0.0 0.0 0.0 0.0 98-99 1.8125 0.0 0.0 0.0 0.0 100-101 2.0875 0.0 0.0 0.0 0.0 102-103 2.2625 0.0 0.0 0.0 0.0 104-105 2.4875 0.0 0.0 0.0 0.0 106-107 3.0999999999999996 0.0 0.0 0.0 0.0 108-109 3.55 0.0 0.0 0.0 0.0 110-111 4.050000000000001 0.0 0.0 0.0 0.0 112-113 4.475 0.0 0.0 0.0 0.0 114-115 4.65 0.0 0.0 0.0 0.0 116-117 5.2 0.0 0.0 0.0 0.0 118-119 5.5625 0.0 0.0 0.0 0.0 120-121 5.85 0.0 0.0 0.0 0.0 122-123 6.35 0.0 0.0 0.0 0.0 124-125 6.675 0.0 0.0 0.0 0.0 126-127 7.375 0.0 0.0 0.0 0.0 128-129 8.162500000000001 0.0 0.0 0.0 0.0 130-131 8.5625 0.0 0.0 0.0 0.0 132-133 9.1 0.0 0.0 0.0 0.0 134-135 9.6125 0.0 0.0 0.0 0.0 136-137 9.962499999999999 0.0 0.0 0.0 0.0 138-139 10.4875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AATCTAA 10 0.006830828 145.0 4 AAATCTA 10 0.006830828 145.0 3 TCTAAGT 10 0.006830828 145.0 6 >>END_MODULE Read 986249 spots for SRR13695465.sra Written 986249 spots for SRR13695465.sra Read 986249 spots for SRR13695465.sra Written 986249 spots for SRR13695465.sra Read 986249 spots for SRR13695465.sra Written 986249 spots for SRR13695465.sra Read 986249 spots for SRR13695465.sra Written 986249 spots for SRR13695465.sra Read 986249 spots for SRR13695465.sra Written 986249 spots for SRR13695465.sra Read 986249 spots for SRR13695465.sra Written 986249 spots for SRR13695465.sra Read 986249 spots for SRR13695465.sra Written 986249 spots for SRR13695465.sra Read 986249 spots for SRR13695465.sra Written 986249 spots for SRR13695465.sra Read 986249 spots for SRR13695465.sra Written 986249 spots for SRR13695465.sra Read 986249 spots for SRR13695465.sra Written 986249 spots for SRR13695465.sra Read 986249 spots for SRR13695465.sra Written 986249 spots for SRR13695465.sra Read 986249 spots for SRR13695465.sra Written 986249 spots for SRR13695465.sra Read 986249 spots for SRR13695465.sra Written 986249 spots for SRR13695465.sra Read 986249 spots for SRR13695465.sra Written 986249 spots for SRR13695465.sra Read 986249 spots for SRR13695465.sra Written 986249 spots for SRR13695465.sra Read 986249 spots for SRR13695465.sra Written 986249 spots for SRR13695465.sra Read 986249 spots for SRR13695465.sra Written 986249 spots for SRR13695465.sra Read 986261 spots for SRR13695465.sra Written 986261 spots for SRR13695465.sra Read 986249 spots for SRR13695465.sra Written 986249 spots for SRR13695465.sra Read 986249 spots for SRR13695465.sra Written 986249 spots for SRR13695465.sra SRR ids: ['SRR13695465.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_v171sw9g SRR13695465.sra spots: 19724992 blocks: [[1, 986249], [986250, 1972498], [1972499, 2958747], [2958748, 3944996], [3944997, 4931245], [4931246, 5917494], [5917495, 6903743], [6903744, 7889992], [7889993, 8876241], [8876242, 9862490], [9862491, 10848739], [10848740, 11834988], [11834989, 12821237], [12821238, 13807486], [13807487, 14793735], [14793736, 15779984], [15779985, 16766233], [16766234, 17752482], [17752483, 18738731], [18738732, 19724992]] SRR13695465 file size 6681714 SRR13695465 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695465 SRR13695465_1.fastq SRR13695465_2.fastq Input file: SRR13695465_1.fastq Paired file: SRR13695465_2.fastq trimmed: SRR13695465-trimmed-pair1.fastq, SRR13695465-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 05:21:15 2025 >> started Wed Feb 12 05:21:38 2025 >> done (23.093s) 19724992 read pairs processed; of these: 115 ( 0.00%) short read pairs filtered out after trimming by size control 4768 ( 0.02%) empty read pairs filtered out after trimming by size control 19720109 (99.98%) read pairs available; of these: 2669438 (13.54%) trimmed read pairs available after processing 17050671 (86.46%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 3 0.00% 19 1 0.00% 20 2 0.00% 21 2 0.00% 22 0 0.00% 23 6 0.00% 24 0 0.00% 25 1 0.00% 26 1 0.00% 27 3 0.00% 28 5 0.00% 29 4 0.00% 30 4 0.00% 31 2 0.00% 32 2 0.00% 33 11 0.00% 34 8 0.00% 35 5 0.00% 36 10 0.00% 37 16 0.00% 38 19 0.00% 39 26 0.00% 40 27 0.00% 41 35 0.00% 42 19 0.00% 43 31 0.00% 44 41 0.00% 45 45 0.00% 46 52 0.00% 47 71 0.00% 48 74 0.00% 49 140 0.00% 50 152 0.00% 51 155 0.00% 52 198 0.00% 53 173 0.00% 54 180 0.00% 55 236 0.00% 56 272 0.00% 57 307 0.00% 58 372 0.00% 59 417 0.00% 60 482 0.00% 61 628 0.00% 62 607 0.00% 63 717 0.00% 64 879 0.00% 65 923 0.00% 66 1046 0.01% 67 1126 0.01% 68 1360 0.01% 69 1574 0.01% 70 1881 0.01% 71 1991 0.01% 72 2261 0.01% 73 2660 0.01% 74 2981 0.02% 75 3350 0.02% 76 3671 0.02% 77 3820 0.02% 78 4416 0.02% 79 5134 0.03% 80 5417 0.03% 81 5990 0.03% 82 6625 0.03% 83 7416 0.04% 84 8180 0.04% 85 8909 0.05% 86 9418 0.05% 87 9939 0.05% 88 10688 0.05% 89 11261 0.06% 90 12104 0.06% 91 13087 0.07% 92 13642 0.07% 93 15055 0.08% 94 15932 0.08% 95 17003 0.09% 96 17491 0.09% 97 18494 0.09% 98 19277 0.10% 99 20341 0.10% 100 21166 0.11% 101 21784 0.11% 102 22889 0.12% 103 24189 0.12% 104 25350 0.13% 105 25892 0.13% 106 27339 0.14% 107 28050 0.14% 108 28789 0.15% 109 30238 0.15% 110 31358 0.16% 111 31368 0.16% 112 33290 0.17% 113 33926 0.17% 114 35004 0.18% 115 36802 0.19% 116 37774 0.19% 117 38897 0.20% 118 40109 0.20% 119 40768 0.21% 120 41645 0.21% 121 42559 0.22% 122 43529 0.22% 123 45074 0.23% 124 45979 0.23% 125 47485 0.24% 126 48905 0.25% 127 50018 0.25% 128 50783 0.26% 129 51668 0.26% 130 52782 0.27% 131 53156 0.27% 132 53685 0.27% 133 55490 0.28% 134 56131 0.28% 135 57049 0.29% 136 58036 0.29% 137 59032 0.30% 138 60749 0.31% 139 62044 0.31% 140 61904 0.31% 141 62816 0.32% 142 63702 0.32% 143 63879 0.32% 144 64669 0.33% 145 65983 0.33% 146 67546 0.34% 147 68158 0.35% 148 68958 0.35% 149 69824 0.35% 150 70314 0.36% 151 17050671 86.46% 19720109 reads passed initial QC criterion=sequence-density sequence-density=0.33 sequence-density-rank=1 fanout-score=2.23 fanout-score-rank=26 prefix-density=0.34 prefix-fanout=2.1 sequence=TGAGATGCCTCAGTGCATCCAAACATGGGTAG criterion=fanout-score sequence-density=0.01 sequence-density-rank=33 fanout-score=484.46 fanout-score-rank=1 prefix-density=0.26 prefix-fanout=17.4 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGAGTATGAATGTGTTCTCTG criterion=sequence-density sequence-density=0.44 sequence-density-rank=1 fanout-score=2.09 fanout-score-rank=28 prefix-density=0.44 prefix-fanout=2.1 sequence=TGTAAGAGATGGCTTCCTC criterion=fanout-score sequence-density=0.13 sequence-density-rank=17 fanout-score=39.30 fanout-score-rank=1 prefix-density=0.38 prefix-fanout=13.0 sequence=AAAGAAAAGAAAA SRR13695465 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 05:22:18 Started mapping on | Feb 12 05:22:18 Finished on | Feb 12 05:24:12 Mapping speed, Million of reads per hour | 622.74 Number of input reads | 19720109 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 18691111 Uniquely mapped reads % | 94.78% Average mapped length | 293.56 Number of splices: Total | 18336260 Number of splices: Annotated (sjdb) | 17928795 Number of splices: GT/AG | 17961815 Number of splices: GC/AG | 300024 Number of splices: AT/AC | 10591 Number of splices: Non-canonical | 63830 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 2.78 Insertion rate per base | 0.02% Insertion average length | 2.33 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 462309 % of reads mapped to multiple loci | 2.34% Number of reads mapped to too many loci | 72809 % of reads mapped to too many loci | 0.37% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.41% % of reads unmapped: other | 0.10% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 566928 566928 566928 N_multimapping 462309 462309 462309 N_noFeature 755547 18268158 1025704 N_ambiguous 268532 1989 114321 UnstrandedReadsAssigned:17667032 PositiveStrandReadsAssigned:420964 NegativeStrandReadsAssigned:17551086 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695465 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695465-trimmed-pair1.fastq SRR13695465-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 19,720,109 reads, 17,545,374 reads pseudoaligned [quant] estimated average fragment length: 243.834 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,100 rounds 52401 SRR13695465.ke.tsv 34699 SRR13695465.se.tsv 87100 total ==> SRR13695465.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1775.17 629 19.969 Potri.005G024800.1.v4.1 1035 792.166 281 19.991 Potri.004G059700.1.v4.1 961 718.238 1 0.0784649 Potri.007G009000.2.v4.1 1416 1173.17 0 0 Potri.003G141000.2.v4.1 2943 2700.17 960.874 20.0549 Potri.016G087400.1.v4.1 270 88.2249 916 585.124 Potri.015G069301.1.v4.1 564 329.647 0 0 Potri.010G195200.1.v4.1 1773 1530.17 94.8585 3.49366 Potri.012G127500.1.v4.1 977 734.208 90 6.90824 ==> SRR13695465.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 181 Potri.001G233950.v4.1 3 Potri.001G122700.v4.1 286 Potri.001G212900.v4.1 4 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 25 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 8 SRR13695465 completed mapping pipeline successfully