Starting /dee2/code/volunteer_pipeline.sh SRR13695466 current disk space = 3048959979520 free memory = 1419698840 SRR13695466 SRAfilesize e04cc13c25f6ca9dc7283c4f33f1261e SRR13695466.sra SRR13695466.sra file validated SRR13695466 is paired end SRR13695466 is conventional basespace SRR13695466 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695466_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.538 37.0 37.0 37.0 37.0 37.0 2 36.26525 37.0 37.0 37.0 37.0 37.0 3 36.526 37.0 37.0 37.0 37.0 37.0 4 36.538 37.0 37.0 37.0 37.0 37.0 5 36.542 37.0 37.0 37.0 37.0 37.0 6 36.5775 37.0 37.0 37.0 37.0 37.0 7 36.542 37.0 37.0 37.0 37.0 37.0 8 36.533 37.0 37.0 37.0 37.0 37.0 9 36.4555 37.0 37.0 37.0 37.0 37.0 10-14 36.538799999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.5178 37.0 37.0 37.0 37.0 37.0 20-24 36.46759999999999 37.0 37.0 37.0 37.0 37.0 25-29 36.4297 37.0 37.0 37.0 37.0 37.0 30-34 36.478300000000004 37.0 37.0 37.0 37.0 37.0 35-39 36.4086 37.0 37.0 37.0 37.0 37.0 40-44 36.462599999999995 37.0 37.0 37.0 37.0 37.0 45-49 36.3768 37.0 37.0 37.0 37.0 37.0 50-54 36.3634 37.0 37.0 37.0 37.0 37.0 55-59 36.3419 37.0 37.0 37.0 37.0 37.0 60-64 36.3514 37.0 37.0 37.0 37.0 37.0 65-69 36.317499999999995 37.0 37.0 37.0 37.0 37.0 70-74 36.3711 37.0 37.0 37.0 37.0 37.0 75-79 36.2747 37.0 37.0 37.0 37.0 37.0 80-84 36.152699999999996 37.0 37.0 37.0 37.0 37.0 85-89 36.2505 37.0 37.0 37.0 37.0 37.0 90-94 36.1485 37.0 37.0 37.0 37.0 37.0 95-99 36.1056 37.0 37.0 37.0 37.0 37.0 100-104 36.1063 37.0 37.0 37.0 37.0 37.0 105-109 36.184900000000006 37.0 37.0 37.0 37.0 37.0 110-114 36.0926 37.0 37.0 37.0 37.0 37.0 115-119 36.061099999999996 37.0 37.0 37.0 37.0 37.0 120-124 35.900800000000004 37.0 37.0 37.0 37.0 37.0 125-129 35.918899999999994 37.0 37.0 37.0 37.0 37.0 130-134 35.9123 37.0 37.0 37.0 37.0 37.0 135-139 35.8432 37.0 37.0 37.0 37.0 37.0 140-144 35.833600000000004 37.0 37.0 37.0 37.0 37.0 145-149 35.67210000000001 37.0 37.0 37.0 37.0 37.0 150-151 35.50875 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 2.0 25 2.0 26 2.0 27 12.0 28 17.0 29 25.0 30 31.0 31 35.0 32 45.0 33 66.0 34 126.0 35 345.0 36 2913.0 37 379.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.7 11.55 9.125 42.625 2 18.99020346646571 13.865862848530519 35.74478774177343 31.399145943230344 3 18.925 16.2 26.05 38.824999999999996 4 22.825 25.95 22.175 29.049999999999997 5 23.075000000000003 31.3 24.9 20.724999999999998 6 20.575 34.949999999999996 23.474999999999998 21.0 7 15.65 27.85 40.975 15.525 8 17.349999999999998 26.875 32.275 23.5 9 18.875 21.95 35.775 23.400000000000002 10-14 19.3 29.86 27.694999999999997 23.145 15-19 20.474999999999998 28.435 27.725 23.365 20-24 19.655 28.16 28.775000000000002 23.41 25-29 19.895 28.754999999999995 27.900000000000002 23.45 30-34 20.505000000000003 27.58 27.93 23.985 35-39 19.994999999999997 28.494999999999997 27.955000000000002 23.555 40-44 20.285 27.800000000000004 27.639999999999997 24.275 45-49 20.244999999999997 28.055000000000003 27.96 23.74 50-54 20.73 28.384999999999998 27.845 23.04 55-59 20.695 28.310000000000002 27.694999999999997 23.3 60-64 19.615 29.235 27.375 23.775 65-69 20.119999999999997 28.775000000000002 27.779999999999998 23.325000000000003 70-74 19.5 28.765 27.455000000000002 24.279999999999998 75-79 20.74 27.725 27.76 23.775 80-84 20.115 27.900000000000002 28.110000000000003 23.875 85-89 19.955000000000002 28.915000000000003 27.62 23.51 90-94 20.075000000000003 28.715000000000003 27.839999999999996 23.369999999999997 95-99 20.44 28.494999999999997 28.125 22.939999999999998 100-104 20.52 28.349999999999998 27.965 23.165 105-109 20.345 28.595 27.655 23.405 110-114 20.145 28.199999999999996 27.495000000000005 24.16 115-119 20.380000000000003 27.61 27.785 24.224999999999998 120-124 21.25 28.095 27.705000000000002 22.95 125-129 20.54 28.52 27.145000000000003 23.794999999999998 130-134 19.82 29.035 26.995 24.15 135-139 20.72 28.275 27.455000000000002 23.549999999999997 140-144 21.175 28.994999999999997 26.11 23.72 145-149 20.615 28.42 26.729999999999997 24.235 150-151 21.0625 27.287499999999998 28.1125 23.5375 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 1.0 17 1.0 18 0.5 19 0.5 20 0.0 21 0.5 22 0.5 23 1.5 24 2.5 25 3.0 26 3.0 27 3.5 28 8.5 29 10.0 30 13.5 31 19.0 32 30.5 33 47.5 34 63.0 35 66.0 36 75.5 37 113.5 38 154.5 39 180.5 40 188.0 41 222.0 42 245.0 43 253.5 44 258.0 45 249.5 46 232.0 47 241.0 48 252.5 49 230.0 50 193.5 51 143.0 52 107.5 53 84.5 54 72.5 55 56.0 56 46.5 57 42.0 58 28.0 59 22.5 60 19.0 61 6.0 62 2.0 63 1.5 64 0.5 65 1.0 66 1.5 67 0.5 68 0.0 69 0.5 70 0.5 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.475 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.975 #Duplication Level Percentage of deduplicated Percentage of total 1 68.51079954528231 45.2 2 19.552860932171274 25.8 3 7.351269420234938 14.549999999999999 4 2.5388404698749527 6.7 5 0.9852216748768473 3.25 6 0.7957559681697612 3.15 7 0.11367942402425162 0.525 8 0.11367942402425162 0.6 9 0.037893141341417205 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGCGGGGTTTGTCTTGTTGTCAGCTCCGCTACTGGTGGCCATTCTTGCTG 9 0.22499999999999998 No Hit GTTCCCTTCAAAGTACGTTGAGCTTCACTTTTGCTTTTTCCAGACTTAAC 8 0.2 No Hit TCTCGATGGAAATAATACTAGTCAACTCAATCACTTGTTTTCATTCATTT 8 0.2 No Hit AGCTTGTGCTAGAAAATTATCGAAACCTCTATTTCCATGAAGAGAGGAAA 8 0.2 No Hit CTTGGATTTTGATGATGTTTTTCCATGAATTCGTAAGAGGCATGAACTGG 7 0.17500000000000002 No Hit TTCTGTAACCTGCTTCCTATATAGTTTGCACCGGCCTTTAGATTCTTGTT 7 0.17500000000000002 No Hit GGGCCTATCAACATCATACCGATTTGAAACCGCAAAACAAATTAAACCAG 7 0.17500000000000002 No Hit GTGGCTGTGGCTGTGGCTGTGCGCTGTTTCGTAGAGGAGGCCAAGCAGAA 6 0.15 No Hit CCCAAGACCACATGAGGTTCACCAACAAAAGGGTTCTTCTCAATGCTGCT 6 0.15 No Hit CTCAGAGCTCTCGTAGAACATCCTAACTGGAGCTACACCAGCAATGATTG 6 0.15 No Hit CACTCACATAGACATGTCAAATACATATACAAGATCCATAAAACAGAAAG 6 0.15 No Hit CTCCAGTCCTGACTTCATCAATAACAGTGGGCTCAAGATCAACAAAGATA 6 0.15 No Hit CCCCTCTGTACTGATCACATGATATTGATTAGCATAAGCAACGGGTGTGT 6 0.15 No Hit GGTAAATAAAAATAAGAAAAGTGCATACGGAAGCAACCAGGGAGAGTATG 6 0.15 No Hit GCGGCAATAAAGCTGATGCACTGCACTTGACGCGTGTTGTCGAATCCGAT 6 0.15 No Hit GTCCTTATCAAACAAGGCTAGAGCCTTTTCAAACAGTTTGTTTTCCCTTG 6 0.15 No Hit CGGAAAACCTTATGGGGATGGTGAGGCAATCGAATAGTGTGACGAGTGGA 6 0.15 No Hit CCGAACTTGACACCGTTGCGGGACAAGAGCTCGGGGAAGACGCATCCAAG 6 0.15 No Hit GTCATCTTTACTTTCTCTTTTTAATGCCTCTGTTGTCAGCTGCTTAGCTG 6 0.15 No Hit TCTACTTCTTCAGGATTTGAAGGTAGCTTTCTATCATCCTGTATTGGTCT 6 0.15 No Hit CCCAGGTTTCTCAACTTCAACATTTACCTCCACAACCTGATTGAAAGCTT 6 0.15 No Hit GTCTCCACTTCCTGCTGCAGCCACTCCTGGCCACTTGTTGGCTACAAGAT 6 0.15 No Hit GGCGTGCTGCCGAGCAGTAGTCAAAGGCTAGCTGACTTCCCCTTGCCTTC 6 0.15 No Hit CAGGAATCCAGTGGCGTCATGCTATCAAATTCCTTGACAACATCAGTGAA 6 0.15 No Hit GGTAAATTTATGCCAACCACACCCCCAACAAAACCCATCCCACTTGTTTA 6 0.15 No Hit CTTGAGTATAGAGGAAGGGGAAAGAGGAGCGGTGTCCAACTGGTAAAGAT 6 0.15 No Hit CCCTGATACACTTGGTCCAAATTCCCAATAATTCCTTCGTTTCCTTGTGT 6 0.15 No Hit CAGAAAAGTTCTGTACACGAATTTGGGTGGATTGAAAACCCTATATGTAG 6 0.15 No Hit GTCCGAATTCATATAAAAGGCAGTGACAGTCCCAGCAGAGTCTCCAGGGA 5 0.125 No Hit GGAGTGAAGAAATTGATTGATGAGAAGAAAGTGGCTGGTGGGATGATACC 5 0.125 No Hit TCTGATATTCCCTCAGGGGCTGCTCTCACGGGTCGGACTTGCACGCGCCC 5 0.125 No Hit CTTGAAGGTGTGTATGATACAGGAGATGAAGAGTTTAACAAAGATGAATC 5 0.125 No Hit GTAACAAAGAACAAAAATAGTCTCCATGGCTATAAATCATTTAACACGTT 5 0.125 No Hit GACTAATTGAGAATTTTATTGCGCATACAGAGATTACATAACTCCGAATA 5 0.125 No Hit TCCAAATCCACAAAGGTCAAATTTGTTCCATATGCTATACGGTCGAATTC 5 0.125 No Hit ACCAAGAGTTGCAGAATTCTCACAGCGACATCGAACCAAACCAGCTTTTG 5 0.125 No Hit GCTGTGTCGATCCTTAGATAATTTAGCTAGACAGGAATACGATGAATGGA 5 0.125 No Hit CCGTCTTGTCTCCACTGTGGAATGAAAAGCACTTCATTGCCCCCAACGAA 5 0.125 No Hit GCCTCTCTTGCGATATCAATCTCCGGCACAGCTATCGCTTAGAAGCTCAC 5 0.125 No Hit AGCTGTAGAACATAGATTAATTTTCTCGAACAAGATTATGTATCTGATGG 5 0.125 No Hit CGCATAGAGCGGGTTTTGGGGTCATAGTGGGCAGAGTCAACATCAAGATT 5 0.125 No Hit GTTACGCTCTTTCAATTCCAAACCATTCAGGCATGCACTTTCTGCTGAGG 5 0.125 No Hit AGTCACTGTCCTTGCTACCAGCTTTGGCCGTTCCTGTGTTAGCTTTCCAA 5 0.125 No Hit CAGAACTCGAGAAGAATTATGTAACAAGGGAAGGGACCGCTACCCTCTGC 5 0.125 No Hit GCCACCACCAGTTCCATAAGGCGTATCGGTCTTGATCTTCTTGACACCAC 5 0.125 No Hit GGCCTTTTGCGCATAGTTCGAGCGCCTATATTTCTCAACAAGTCTTGATT 5 0.125 No Hit CAGCATCAAATGAAGGTGCTCCCCATTGAAAGGGATATGGAACTCTTATC 5 0.125 No Hit CTCGGAATTGGCAACCGAACTAATGGGTCTGGCAGCAAAGCAGGAAAATC 5 0.125 No Hit GGAGATAAGAGGCGTGTGTGGCGTTGCCTGTGGAGAGGTCGGATTTGGCG 5 0.125 No Hit CTCATGGTGATCAGCTCATTCCAGCAATATGATGCTCCACCAGATTCCAA 5 0.125 No Hit CTGGTTCATGTTAACTTTATGTAGCTGGTGACAAACTGACAATACTAGCA 5 0.125 No Hit GTCCTGGCTTCGAGTATGAAACTGAAGAACAATGCTTATGAGGAAAACGC 5 0.125 No Hit GCGCTTGCTAATGTCCTCCCATGTAAGGCATTCCTGATCTAAACCATGGT 5 0.125 No Hit CGCACAATGATAGCTCCTCGAGTGGAATAATAAAATCAAATAGAGAACTG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.075 0.0 0.0 0.0 0.0 84-85 0.125 0.0 0.0 0.0 0.0 86-87 0.2 0.0 0.0 0.0 0.0 88-89 0.25 0.0 0.0 0.0 0.0 90-91 0.3375 0.0 0.0 0.0 0.0 92-93 0.45 0.0 0.0 0.0 0.0 94-95 0.6625 0.0 0.0 0.0 0.0 96-97 0.7375 0.0 0.0 0.0 0.0 98-99 0.875 0.0 0.0 0.0 0.0 100-101 1.0 0.0 0.0 0.0 0.0 102-103 1.0875 0.0 0.0 0.0 0.0 104-105 1.2125 0.0 0.0 0.0 0.0 106-107 1.3375 0.0 0.0 0.0 0.0 108-109 1.525 0.0 0.0 0.0 0.0 110-111 1.6625 0.0 0.0 0.0 0.0 112-113 2.05 0.0 0.0 0.0 0.0 114-115 2.4124999999999996 0.0 0.0 0.0 0.0 116-117 2.625 0.0 0.0 0.0 0.0 118-119 2.95 0.0 0.0 0.0 0.0 120-121 3.2625 0.0 0.0 0.0 0.0 122-123 3.575 0.0 0.0 0.0 0.0 124-125 3.75 0.0 0.0 0.0 0.0 126-127 3.9000000000000004 0.0 0.0 0.0 0.0 128-129 4.3 0.0 0.0 0.0 0.0 130-131 4.862500000000001 0.0 0.0 0.0 0.0 132-133 5.3 0.0 0.0 0.0 0.0 134-135 5.85 0.0 0.0 0.0 0.0 136-137 6.237500000000001 0.0 0.0 0.0 0.0 138-139 6.7875 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CACATGA 10 0.006830828 145.0 9 TTACTTT 10 0.006830828 145.0 9 GATATTT 10 0.006830828 145.0 3 ACCACAT 10 0.006830828 145.0 7 TTACATC 10 0.006830828 145.0 8 TGATATT 10 0.006830828 145.0 2 TACATCA 10 0.006830828 145.0 9 ATTACAT 10 0.006830828 145.0 7 TACAAGA 10 0.006830828 145.0 145 TATTTTA 10 0.006830828 145.0 5 TATTACA 10 0.006830828 145.0 6 ATACTAT 10 0.006830828 145.0 2 CATACTA 10 0.006830828 145.0 1 TACTATT 10 0.006830828 145.0 3 ACTATTA 10 0.006830828 145.0 4 CTATTAC 10 0.006830828 145.0 5 >>END_MODULE SRR13695466 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695466_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.172 37.0 37.0 37.0 37.0 37.0 2 36.127 37.0 37.0 37.0 37.0 37.0 3 36.248 37.0 37.0 37.0 37.0 37.0 4 36.0955 37.0 37.0 37.0 37.0 37.0 5 36.36 37.0 37.0 37.0 37.0 37.0 6 36.3215 37.0 37.0 37.0 37.0 37.0 7 36.237 37.0 37.0 37.0 37.0 37.0 8 36.345 37.0 37.0 37.0 37.0 37.0 9 36.363 37.0 37.0 37.0 37.0 37.0 10-14 36.2872 37.0 37.0 37.0 37.0 37.0 15-19 36.2082 37.0 37.0 37.0 37.0 37.0 20-24 36.224599999999995 37.0 37.0 37.0 37.0 37.0 25-29 36.1929 37.0 37.0 37.0 37.0 37.0 30-34 36.1601 37.0 37.0 37.0 37.0 37.0 35-39 36.1551 37.0 37.0 37.0 37.0 37.0 40-44 36.1404 37.0 37.0 37.0 37.0 37.0 45-49 36.1584 37.0 37.0 37.0 37.0 37.0 50-54 36.119600000000005 37.0 37.0 37.0 37.0 37.0 55-59 36.0786 37.0 37.0 37.0 37.0 37.0 60-64 35.993100000000005 37.0 37.0 37.0 37.0 37.0 65-69 35.970600000000005 37.0 37.0 37.0 37.0 37.0 70-74 35.9565 37.0 37.0 37.0 37.0 37.0 75-79 36.03869999999999 37.0 37.0 37.0 37.0 37.0 80-84 35.9377 37.0 37.0 37.0 37.0 37.0 85-89 35.8643 37.0 37.0 37.0 37.0 37.0 90-94 35.7894 37.0 37.0 37.0 37.0 37.0 95-99 35.776399999999995 37.0 37.0 37.0 37.0 37.0 100-104 35.797 37.0 37.0 37.0 37.0 37.0 105-109 35.799400000000006 37.0 37.0 37.0 37.0 37.0 110-114 35.7564 37.0 37.0 37.0 37.0 37.0 115-119 35.7088 37.0 37.0 37.0 37.0 37.0 120-124 35.652 37.0 37.0 37.0 37.0 37.0 125-129 35.660000000000004 37.0 37.0 37.0 37.0 37.0 130-134 35.539500000000004 37.0 37.0 37.0 37.0 37.0 135-139 35.487700000000004 37.0 37.0 37.0 37.0 37.0 140-144 35.3906 37.0 37.0 37.0 34.6 37.0 145-149 35.3125 37.0 37.0 37.0 32.2 37.0 150-151 34.93775 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 1.0 14 1.0 15 0.0 16 0.0 17 1.0 18 0.0 19 2.0 20 1.0 21 0.0 22 2.0 23 1.0 24 2.0 25 7.0 26 12.0 27 8.0 28 16.0 29 24.0 30 31.0 31 42.0 32 89.0 33 95.0 34 204.0 35 567.0 36 2692.0 37 202.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 35.062656641604015 20.30075187969925 14.385964912280702 30.250626566416038 2 26.35 26.775 31.7 15.174999999999999 3 21.175 26.875 32.25 19.7 4 23.75 35.3 23.849999999999998 17.1 5 25.05 35.725 22.55 16.675 6 19.875 38.9 22.325 18.9 7 20.575 22.15 39.95 17.325 8 19.875 27.325 29.2 23.599999999999998 9 23.974999999999998 23.599999999999998 31.874999999999996 20.549999999999997 10-14 22.755 30.055 26.195 20.995 15-19 23.45 27.985 28.225 20.34 20-24 22.900000000000002 28.99 28.005000000000003 20.105 25-29 22.165000000000003 29.075 27.96 20.8 30-34 22.825 29.09 27.339999999999996 20.745 35-39 21.740000000000002 29.505 27.425 21.33 40-44 22.445 28.425 27.939999999999998 21.19 45-49 22.55 28.310000000000002 27.935 21.205 50-54 22.865 27.384999999999998 28.735 21.015 55-59 22.935 27.825 28.155 21.085 60-64 22.035 28.09 27.650000000000002 22.225 65-69 21.985 27.544999999999998 28.83 21.64 70-74 23.505000000000003 28.084999999999997 27.99 20.419999999999998 75-79 23.04 27.284999999999997 28.17 21.505 80-84 22.8 28.63 26.845000000000002 21.725 85-89 23.285 28.24 27.27 21.205 90-94 23.580000000000002 27.425 28.285 20.71 95-99 23.535 27.625 27.83 21.01 100-104 22.994999999999997 28.345 27.735 20.925 105-109 23.34 27.665 27.794999999999998 21.2 110-114 24.15 27.834999999999997 27.76 20.255000000000003 115-119 23.549999999999997 27.855 27.61 20.985 120-124 23.400000000000002 27.805000000000003 28.084999999999997 20.71 125-129 24.404999999999998 27.555000000000003 27.47 20.57 130-134 24.55 27.935 27.57 19.945 135-139 24.365000000000002 28.62 26.495 20.52 140-144 25.085 27.49 27.49 19.935 145-149 24.985 28.15 26.96 19.905 150-151 26.1625 27.987499999999997 25.937500000000004 19.9125 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 0.0 10 0.5 11 0.5 12 0.0 13 0.5 14 0.5 15 0.0 16 0.0 17 0.0 18 0.0 19 1.5 20 5.0 21 4.0 22 1.0 23 1.0 24 2.0 25 3.0 26 4.0 27 6.5 28 9.0 29 11.5 30 13.0 31 17.5 32 29.0 33 38.5 34 44.0 35 65.5 36 95.5 37 124.5 38 151.5 39 181.0 40 221.5 41 235.5 42 255.0 43 258.5 44 255.5 45 282.5 46 273.0 47 243.5 48 214.5 49 181.5 50 166.0 51 139.5 52 97.0 53 79.5 54 78.5 55 60.0 56 39.0 57 35.0 58 22.5 59 16.5 60 15.0 61 5.5 62 3.0 63 4.0 64 1.5 65 0.0 66 0.0 67 0.0 68 0.0 69 0.0 70 0.0 71 0.5 72 0.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.25 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.925 #Duplication Level Percentage of deduplicated Percentage of total 1 69.48076204706761 46.5 2 19.01382144191259 25.45 3 7.134852446768771 14.325 4 2.6522226372805378 7.1 5 0.7844602166604409 2.625 6 0.709749719835637 2.85 7 0.11206574523720583 0.525 8 0.0747104968248039 0.4 9 0.03735524841240195 0.22499999999999998 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTTATCTGTCTACTGTTTCAGTGTTCAAGCTCTTCACTAAAGAGACACCA 9 0.22499999999999998 No Hit GGAAGGGCTGTATGCTATCCATCAACTGAGATTCTACGAGATTACCTGGC 8 0.2 No Hit TGTAAATTAGTTGCAGATTCCTTTTCTGGCATGACATCTACACAGTTTGG 8 0.2 No Hit TCTCTCTCTTCTCCCTAGCTTCACCTCTAAAACAAGCAACCATGGAACTT 7 0.17500000000000002 No Hit ATTGTTTTTTAGTTCTGTTGTCTTCTAGGTGGTCTTATCGTGGTCGCTCA 7 0.17500000000000002 No Hit GGAAAGCATACAAGTTTGACATGAAAACCCTTGTTCTAAAGGTTCCTTCA 7 0.17500000000000002 No Hit GCAATTACCAATGCATTAGAGCGATATCAGGAGATGGATCCAACTTTTTC 6 0.15 No Hit AAAAAAAATCTTAACTGCACCCGCTCATCCAGCAATGGCAGCAGCAACAA 6 0.15 No Hit CTGACATACAAGAAATCGACATAGATAACAACACAGAAGTTCTTGTACTC 6 0.15 No Hit TACAATAAAAGGTCCAAGCATCCAATAAATCTCAAAGGATTTATGGTTGG 6 0.15 No Hit CGCCACCAGCAGCTCAACCACCAACCATCACTCCACCAGACACAGTGGAA 6 0.15 No Hit GGTTTTTTTCTTTTGTTTGTTGTTTGCTACCAATAGAGGTGCTGCTGTCT 6 0.15 No Hit CATTTCTGCAGAGGATTCACTGCTTCAATCTTGATAGGTCTTATTGTGAT 6 0.15 No Hit GGGCATTACAGCAGAAGATGTCAATGGAGCCTTCAGAAAGGCGGCTGAGG 6 0.15 No Hit TTTAGAGAGTTTGATCGAACAAGATTAGATTTGGAAGACGGTGTTGGTTC 6 0.15 No Hit AAATGAGAGAGTGCATTTCGATTCACATTGGTCAAGCCGGTATTCAGGTC 6 0.15 No Hit GAGCTTGAGTGCATTCTTCTAAGTAAAAGAAATCCCTTAATTTCATCACA 6 0.15 No Hit CTTTTGTTTTTGGTAATTATGGGAAATTGTTGCTGTAGATGGGAGCTTTC 6 0.15 No Hit GCAACAGAGTGTTTGGGGAGGGAAAAACAAGGGGACAGGAGTATTGTTTC 6 0.15 No Hit CAGTGATCAGCCCAACTAATATTCTTCTATTTGCAAAAGAGTCCCGTGTC 6 0.15 No Hit GAAGGATGTCGTGCGATATCCCTCGATTTCGCTCGACATGACCAAACTAA 6 0.15 No Hit CAAAACAAAAACAATGTCTCCTGCAGTGCCTCTCTCCGAAATTCAAATCG 6 0.15 No Hit GAAAATTAGTGGACAAAACTGTGGGTGCTGATAAAGATGGCCTGCCAACA 6 0.15 No Hit TGCTTGTTGTTGCTCCTGCTATTCTTTGGGTGCTCTACAATATTCTACAG 6 0.15 No Hit CGTGTTTTCTTGGCATGACCTTGATTGTTATTATAAGCTTTTTGAGGAAT 6 0.15 No Hit GTTTTCTTAATGCACATATTATTGTTGGAGTTCCAATTTTTGTAATCTAA 5 0.125 No Hit GTAAAATGGAAACTCACTCTCTCCATGTCACTGCAGAACAACTTGACGAC 5 0.125 No Hit TGTAAATGGAAGCAGCTACAGCAACATTTATTGGATCTTCAATTGGGATC 5 0.125 No Hit TTTATTTCACAGTACATGCCCCATTTGATAAAGTCAGGAAGACATGTCTT 5 0.125 No Hit CTCCATTGTCCCGTACCACAGCAACAAACTCAACACAAAACAAGAATGGC 5 0.125 No Hit AGTAGTGATAATTCAATATATATTTATGATCTTGAAGCAAATACCTGCAG 5 0.125 No Hit AATATGGATTTTTCTGGCACTTCTATCAGGATGGAAAAATCGAAGCTGAA 5 0.125 No Hit ATTAGTTTATTAAGGTCTTTTTGGGACATTGACTGAAGGGTCTCTTCTCA 5 0.125 No Hit CATGACACAAGAGAAGAGAGATTCTTCCTCCTCCTCCTCCTTTTCTTCCT 5 0.125 No Hit CAAAACCCTCACAATTGAAATCTTCACTTCTCTTCTCCAGCTACAAATCC 5 0.125 No Hit GGGAGCACTGCATAGCTTATAAGCTTGTAAGAGATGGCTTCCTCCTCTAT 5 0.125 No Hit CAGTAATCATAGTACCAATGCCTTCTTCAGACATAATCTCATGAAGCAAA 5 0.125 No Hit TTTGGGTCCATTATCTAGTGGTAAAAGCTGTGAGCTCAAAGCACCAGGGC 5 0.125 No Hit CAACGGTGGTGGAGATGCAGTGACAGCAGCAGCAGGAGTTATGGAGATGA 5 0.125 No Hit CTTTATTCTTTCAAGTGCAAGACATGCATTAGAAGACTATATTCATGCTG 5 0.125 No Hit ATTTCCTCTCCACGTCTCGGCTCGACCGGCAACTTTTCTTCAAGACTTTA 5 0.125 No Hit GTGCAATTTACTCTTTGATTGTGAGAGAGGAAGAGATTTTTATTGAAGAG 5 0.125 No Hit ATTGAGAGCAAGTAAACAATGGGCAAAGAAAACGAGACATCGGAGACACC 5 0.125 No Hit AGAAGAAGGAATCCTTCTGATCACTTTCTACGCTGTATAAATTCAGACTT 5 0.125 No Hit CTTCACTTTATTTCCTCTCTCTCAACAACCCCATTTCCTTTTTCGCCTCC 5 0.125 No Hit GGGTTAGTGAATTAGATCGAAACAAAAATGGAAAAGGCCTGGTGGATTTG 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.05 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.075 0.0 0.0 0.0 0.0 80-81 0.075 0.0 0.0 0.0 0.0 82-83 0.075 0.0 0.0 0.0 0.0 84-85 0.125 0.0 0.0 0.0 0.0 86-87 0.2 0.0 0.0 0.0 0.0 88-89 0.25 0.0 0.0 0.0 0.0 90-91 0.3375 0.0 0.0 0.0 0.0 92-93 0.45 0.0 0.0 0.0 0.0 94-95 0.6625 0.0 0.0 0.0 0.0 96-97 0.7375 0.0 0.0 0.0 0.0 98-99 0.875 0.0 0.0 0.0 0.0 100-101 1.0 0.0 0.0 0.0 0.0 102-103 1.0875 0.0 0.0 0.0 0.0 104-105 1.2125 0.0 0.0 0.0 0.0 106-107 1.35 0.0 0.0 0.0 0.0 108-109 1.55 0.0 0.0 0.0 0.0 110-111 1.6875 0.0 0.0 0.0 0.0 112-113 2.075 0.0 0.0 0.0 0.0 114-115 2.4375 0.0 0.0 0.0 0.0 116-117 2.6500000000000004 0.0 0.0 0.0 0.0 118-119 2.9749999999999996 0.0 0.0 0.0 0.0 120-121 3.2875 0.0 0.0 0.0 0.0 122-123 3.575 0.0 0.0 0.0 0.0 124-125 3.75 0.0 0.0 0.0 0.0 126-127 3.9000000000000004 0.0 0.0 0.0 0.0 128-129 4.325 0.0 0.0 0.0 0.0 130-131 4.887499999999999 0.0 0.0 0.0 0.0 132-133 5.325 0.0 0.0 0.0 0.0 134-135 5.875 0.0 0.0 0.0 0.0 136-137 6.262499999999999 0.0 0.0 0.0 0.0 138-139 6.8 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACAGTG 10 0.006830828 145.0 3 GTGCAAT 10 0.006830828 145.0 1 TGCAATT 10 0.006830828 145.0 2 TTACTCT 10 0.006830828 145.0 8 AATTTAC 10 0.006830828 145.0 5 CAACTCA 10 0.006830828 145.0 3 AGCTAGC 10 0.006830828 145.0 9 CAATTTA 10 0.006830828 145.0 4 GCAATTT 10 0.006830828 145.0 3 TCAGCTA 10 0.006830828 145.0 7 ATTACAG 10 0.006830828 145.0 1 GCCAACT 10 0.006830828 145.0 1 TTTACTC 10 0.006830828 145.0 7 TACTCTT 10 0.006830828 145.0 9 AGAGAGA 65 0.0076375785 13.384615 80-84 >>END_MODULE Read 953477 spots for SRR13695466.sra Written 953477 spots for SRR13695466.sra Read 953477 spots for SRR13695466.sra Written 953477 spots for SRR13695466.sra Read 953477 spots for SRR13695466.sra Written 953477 spots for SRR13695466.sra Read 953477 spots for SRR13695466.sra Written 953477 spots for SRR13695466.sra Read 953477 spots for SRR13695466.sra Written 953477 spots for SRR13695466.sra Read 953477 spots for SRR13695466.sra Written 953477 spots for SRR13695466.sra Read 953477 spots for SRR13695466.sra Written 953477 spots for SRR13695466.sra Read 953477 spots for SRR13695466.sra Written 953477 spots for SRR13695466.sra Read 953477 spots for SRR13695466.sra Written 953477 spots for SRR13695466.sra Read 953477 spots for SRR13695466.sra Written 953477 spots for SRR13695466.sra Read 953477 spots for SRR13695466.sra Written 953477 spots for SRR13695466.sra Read 953477 spots for SRR13695466.sra Written 953477 spots for SRR13695466.sra Read 953477 spots for SRR13695466.sra Written 953477 spots for SRR13695466.sra Read 953477 spots for SRR13695466.sra Written 953477 spots for SRR13695466.sra Read 953477 spots for SRR13695466.sra Written 953477 spots for SRR13695466.sra Read 953477 spots for SRR13695466.sra Written 953477 spots for SRR13695466.sra Read 953477 spots for SRR13695466.sra Written 953477 spots for SRR13695466.sra Read 953477 spots for SRR13695466.sra Written 953477 spots for SRR13695466.sra Read 953477 spots for SRR13695466.sra Written 953477 spots for SRR13695466.sra Read 953486 spots for SRR13695466.sra Written 953486 spots for SRR13695466.sra SRR ids: ['SRR13695466.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_p0ht6khp SRR13695466.sra spots: 19069549 blocks: [[1, 953477], [953478, 1906954], [1906955, 2860431], [2860432, 3813908], [3813909, 4767385], [4767386, 5720862], [5720863, 6674339], [6674340, 7627816], [7627817, 8581293], [8581294, 9534770], [9534771, 10488247], [10488248, 11441724], [11441725, 12395201], [12395202, 13348678], [13348679, 14302155], [14302156, 15255632], [15255633, 16209109], [16209110, 17162586], [17162587, 18116063], [18116064, 19069549]] SRR13695466 file size 6458966 SRR13695466 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695466 SRR13695466_1.fastq SRR13695466_2.fastq Input file: SRR13695466_1.fastq Paired file: SRR13695466_2.fastq trimmed: SRR13695466-trimmed-pair1.fastq, SRR13695466-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 05:24:27 2025 >> started Wed Feb 12 05:24:51 2025 >> done (23.539s) 19069549 read pairs processed; of these: 138 ( 0.00%) short read pairs filtered out after trimming by size control 1101 ( 0.01%) empty read pairs filtered out after trimming by size control 19068310 (99.99%) read pairs available; of these: 1819451 ( 9.54%) trimmed read pairs available after processing 17248859 (90.46%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 1 0.00% 20 0 0.00% 21 0 0.00% 22 5 0.00% 23 0 0.00% 24 1 0.00% 25 4 0.00% 26 1 0.00% 27 4 0.00% 28 5 0.00% 29 2 0.00% 30 5 0.00% 31 5 0.00% 32 2 0.00% 33 3 0.00% 34 4 0.00% 35 2 0.00% 36 5 0.00% 37 3 0.00% 38 11 0.00% 39 9 0.00% 40 8 0.00% 41 3 0.00% 42 6 0.00% 43 22 0.00% 44 9 0.00% 45 13 0.00% 46 13 0.00% 47 13 0.00% 48 8 0.00% 49 24 0.00% 50 31 0.00% 51 25 0.00% 52 23 0.00% 53 36 0.00% 54 59 0.00% 55 55 0.00% 56 45 0.00% 57 63 0.00% 58 81 0.00% 59 83 0.00% 60 122 0.00% 61 126 0.00% 62 142 0.00% 63 153 0.00% 64 194 0.00% 65 240 0.00% 66 264 0.00% 67 348 0.00% 68 311 0.00% 69 365 0.00% 70 483 0.00% 71 611 0.00% 72 693 0.00% 73 756 0.00% 74 1001 0.01% 75 1043 0.01% 76 1093 0.01% 77 1313 0.01% 78 1415 0.01% 79 1610 0.01% 80 1965 0.01% 81 2106 0.01% 82 2446 0.01% 83 2825 0.01% 84 3281 0.02% 85 3726 0.02% 86 3637 0.02% 87 4136 0.02% 88 4665 0.02% 89 4836 0.03% 90 5360 0.03% 91 5812 0.03% 92 6189 0.03% 93 6891 0.04% 94 7653 0.04% 95 8082 0.04% 96 8842 0.05% 97 9427 0.05% 98 9919 0.05% 99 10518 0.06% 100 11039 0.06% 101 11500 0.06% 102 12449 0.07% 103 13103 0.07% 104 14142 0.07% 105 14735 0.08% 106 15763 0.08% 107 16636 0.09% 108 17037 0.09% 109 17730 0.09% 110 18682 0.10% 111 19220 0.10% 112 20368 0.11% 113 20560 0.11% 114 21934 0.12% 115 23138 0.12% 116 24031 0.13% 117 25009 0.13% 118 26658 0.14% 119 26691 0.14% 120 27454 0.14% 121 28561 0.15% 122 29660 0.16% 123 30802 0.16% 124 31719 0.17% 125 32340 0.17% 126 33866 0.18% 127 34368 0.18% 128 36138 0.19% 129 36422 0.19% 130 38148 0.20% 131 38376 0.20% 132 39088 0.20% 133 40725 0.21% 134 41381 0.22% 135 42450 0.22% 136 43646 0.23% 137 44699 0.23% 138 45525 0.24% 139 47444 0.25% 140 47449 0.25% 141 48595 0.25% 142 50176 0.26% 143 50404 0.26% 144 51501 0.27% 145 53508 0.28% 146 53381 0.28% 147 54730 0.29% 148 55608 0.29% 149 57095 0.30% 150 58475 0.31% 151 17248859 90.46% 19068310 reads passed initial QC criterion=sequence-density sequence-density=0.40 sequence-density-rank=1 fanout-score=2.22 fanout-score-rank=35 prefix-density=0.42 prefix-fanout=2.1 sequence=TGAGATGCCTCAGTGCATCCAAACATGGGTAG criterion=fanout-score sequence-density=0.03 sequence-density-rank=39 fanout-score=98.60 fanout-score-rank=1 prefix-density=0.33 prefix-fanout=10.3 sequence=TGCTGCTGCTGCATTTATTAGAGAAGGAGATGCTGGTAATCTCCATTTCACAAGTTCATTAATCTCGATCGAAAATATGGCTCATTTAAGCATATACAAAGTACATCTGGGAAAGAAAGTTAAGAACCAAGATAGGGTCACTGATATTTGGATGATGTTCATACGGAGAGGGAGGGAGAAAGGCTGTACTCAAGCCCCCAGAGCTCAAAACTGCCTTTGACAAAATCATAATAACCACCCTTCAGTCCTAGAGTTTTGTTCACCAAGCCATCTCTCACAAACGGGTAGGTTAGCAAGTGTCCAAGGGACACGTTCACTGCCTCCTTTTCACATTGTGTACAGAGGTCTGGGAAAGGTGCATTGGCATGTTCTGCTAAAACCTTAGTCTTGGCAGGGTAGCAAACTTTGACCCAGTCTTCTATGAAATCAGTTGATGTTGTTCCATCATAAGGGAAGGACATGAGGCCCTTAATTCCACCACAGGCGCTGTGTCCAATGACCACAATGTATT criterion=sequence-density sequence-density=0.39 sequence-density-rank=1 fanout-score=2.06 fanout-score-rank=35 prefix-density=0.39 prefix-fanout=2.1 sequence=CTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAGTGAACAAACAAAAGTGTCCGTGAGTGTCTTTATCTTTGT criterion=fanout-score sequence-density=0.17 sequence-density-rank=10 fanout-score=29.09 fanout-score-rank=1 prefix-density=0.45 prefix-fanout=11.0 sequence=AAAGAAAAGAAAA SRR13695466 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 05:25:34 Started mapping on | Feb 12 05:25:34 Finished on | Feb 12 05:27:21 Mapping speed, Million of reads per hour | 641.55 Number of input reads | 19068310 Average input read length | 297 UNIQUE READS: Uniquely mapped reads number | 18066305 Uniquely mapped reads % | 94.75% Average mapped length | 296.36 Number of splices: Total | 17489087 Number of splices: Annotated (sjdb) | 17140773 Number of splices: GT/AG | 17148727 Number of splices: GC/AG | 278686 Number of splices: AT/AC | 9689 Number of splices: Non-canonical | 51985 Mismatch rate per base, % | 0.36% Deletion rate per base | 0.03% Deletion average length | 2.79 Insertion rate per base | 0.02% Insertion average length | 2.38 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 461641 % of reads mapped to multiple loci | 2.42% Number of reads mapped to too many loci | 58396 % of reads mapped to too many loci | 0.31% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.43% % of reads unmapped: other | 0.09% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 540586 540586 540586 N_multimapping 461641 461641 461641 N_noFeature 660048 17687298 905127 N_ambiguous 241106 1506 106189 UnstrandedReadsAssigned:17165151 PositiveStrandReadsAssigned:377501 NegativeStrandReadsAssigned:17054989 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695466 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695466-trimmed-pair1.fastq SRR13695466-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 19,068,310 reads, 17,152,666 reads pseudoaligned [quant] estimated average fragment length: 252.072 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,062 rounds 52401 SRR13695466.ke.tsv 34699 SRR13695466.se.tsv 87100 total ==> SRR13695466.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1766.93 632 21.1745 Potri.005G024800.1.v4.1 1035 783.928 378 28.545 Potri.004G059700.1.v4.1 961 710.008 5 0.416891 Potri.007G009000.2.v4.1 1416 1164.93 0 0 Potri.003G141000.2.v4.1 2943 2691.93 795 17.4831 Potri.016G087400.1.v4.1 270 80.4545 674 495.935 Potri.015G069301.1.v4.1 564 321.337 0 0 Potri.010G195200.1.v4.1 1773 1521.93 74 2.87841 Potri.012G127500.1.v4.1 977 725.979 63 5.13726 ==> SRR13695466.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 816 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 259 Potri.001G212900.v4.1 1 Potri.001G182400.v4.1 1 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR13695466 completed mapping pipeline successfully