Starting /dee2/code/volunteer_pipeline.sh SRR13695467 current disk space = 3049031159808 free memory = 1571395204 SRR13695467 SRAfilesize 09d71af1399c5c1bbf654682e18a1d0b SRR13695467.sra SRR13695467.sra file validated SRR13695467 is paired end SRR13695467 is conventional basespace SRR13695467 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695467_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5565 37.0 37.0 37.0 37.0 37.0 2 36.32125 37.0 37.0 37.0 37.0 37.0 3 36.5285 37.0 37.0 37.0 37.0 37.0 4 36.5135 37.0 37.0 37.0 37.0 37.0 5 36.5145 37.0 37.0 37.0 37.0 37.0 6 36.615 37.0 37.0 37.0 37.0 37.0 7 36.5365 37.0 37.0 37.0 37.0 37.0 8 36.4965 37.0 37.0 37.0 37.0 37.0 9 36.556 37.0 37.0 37.0 37.0 37.0 10-14 36.538799999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.5009 37.0 37.0 37.0 37.0 37.0 20-24 36.4949 37.0 37.0 37.0 37.0 37.0 25-29 36.4094 37.0 37.0 37.0 37.0 37.0 30-34 36.39829999999999 37.0 37.0 37.0 37.0 37.0 35-39 36.406 37.0 37.0 37.0 37.0 37.0 40-44 36.3232 37.0 37.0 37.0 37.0 37.0 45-49 36.25580000000001 37.0 37.0 37.0 37.0 37.0 50-54 36.2568 37.0 37.0 37.0 37.0 37.0 55-59 36.2351 37.0 37.0 37.0 37.0 37.0 60-64 36.263999999999996 37.0 37.0 37.0 37.0 37.0 65-69 36.1811 37.0 37.0 37.0 37.0 37.0 70-74 36.217499999999994 37.0 37.0 37.0 37.0 37.0 75-79 36.2281 37.0 37.0 37.0 37.0 37.0 80-84 36.1243 37.0 37.0 37.0 37.0 37.0 85-89 36.183200000000014 37.0 37.0 37.0 37.0 37.0 90-94 36.120799999999996 37.0 37.0 37.0 37.0 37.0 95-99 36.1298 37.0 37.0 37.0 37.0 37.0 100-104 36.0721 37.0 37.0 37.0 37.0 37.0 105-109 36.0487 37.0 37.0 37.0 37.0 37.0 110-114 36.0261 37.0 37.0 37.0 37.0 37.0 115-119 35.979 37.0 37.0 37.0 37.0 37.0 120-124 35.9386 37.0 37.0 37.0 37.0 37.0 125-129 35.9456 37.0 37.0 37.0 37.0 37.0 130-134 35.9076 37.0 37.0 37.0 37.0 37.0 135-139 35.835499999999996 37.0 37.0 37.0 37.0 37.0 140-144 35.785199999999996 37.0 37.0 37.0 37.0 37.0 145-149 35.5572 37.0 37.0 37.0 37.0 37.0 150-151 35.42425 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 17 1.0 18 0.0 19 0.0 20 1.0 21 0.0 22 0.0 23 0.0 24 1.0 25 3.0 26 7.0 27 6.0 28 16.0 29 17.0 30 25.0 31 41.0 32 83.0 33 77.0 34 146.0 35 324.0 36 2943.0 37 309.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.374999999999996 10.85 13.525 44.25 2 19.482801908109465 15.641476274165203 34.44639718804921 30.429324629676124 3 19.975 17.25 22.525000000000002 40.25 4 21.325 25.174999999999997 23.400000000000002 30.099999999999998 5 24.425 29.275000000000002 24.625 21.675 6 20.549999999999997 34.925 22.825 21.7 7 15.25 26.8 41.0 16.950000000000003 8 19.5 26.125 30.675 23.7 9 17.474999999999998 25.324999999999996 35.449999999999996 21.75 10-14 20.185 29.575000000000003 27.150000000000002 23.09 15-19 21.029999999999998 27.810000000000002 28.15 23.01 20-24 19.02 28.78 28.15 24.05 25-29 19.945 28.310000000000002 27.505000000000003 24.240000000000002 30-34 20.119999999999997 28.999999999999996 26.935 23.945 35-39 20.48 27.815 27.584999999999997 24.12 40-44 20.735 28.64 28.115000000000002 22.509999999999998 45-49 20.565 28.415000000000003 27.685 23.335 50-54 20.580000000000002 28.27 26.935 24.215 55-59 19.97 27.73 27.87 24.43 60-64 21.525 27.785 27.665 23.025000000000002 65-69 20.07 28.345 28.03 23.555 70-74 20.62 28.58 26.905 23.895 75-79 19.685 28.299999999999997 28.16 23.855 80-84 20.94 28.110000000000003 28.015 22.935 85-89 20.625 28.439999999999998 27.67 23.265 90-94 20.625 28.689999999999998 27.139999999999997 23.544999999999998 95-99 20.015 29.14 27.38 23.465 100-104 20.445 27.805000000000003 27.815 23.935000000000002 105-109 20.31 28.59 27.61 23.49 110-114 20.549999999999997 27.985 27.839999999999996 23.625 115-119 21.445 28.499999999999996 27.195000000000004 22.86 120-124 20.93 27.255000000000003 27.785 24.03 125-129 20.91 28.215 26.590000000000003 24.285 130-134 21.65 28.76 27.13 22.46 135-139 21.125 27.950000000000003 26.889999999999997 24.035 140-144 21.425 27.560000000000002 27.589999999999996 23.425 145-149 21.55 28.18 26.924999999999997 23.345 150-151 20.8625 28.037499999999998 26.3 24.8 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 0.5 19 1.5 20 2.5 21 1.5 22 1.0 23 1.5 24 4.0 25 7.0 26 6.5 27 8.5 28 13.0 29 21.0 30 22.0 31 20.0 32 32.0 33 40.5 34 54.0 35 62.0 36 68.5 37 109.5 38 134.0 39 144.5 40 173.0 41 190.5 42 224.5 43 255.0 44 270.5 45 285.0 46 274.5 47 253.0 48 223.5 49 203.5 50 206.5 51 152.0 52 102.0 53 87.0 54 58.5 55 58.0 56 56.5 57 44.5 58 32.0 59 25.0 60 25.5 61 14.5 62 2.5 63 4.0 64 4.0 65 2.0 66 1.0 67 1.5 68 2.0 69 4.0 70 3.0 71 1.5 72 2.0 73 1.0 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.42500000000000004 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.425 #Duplication Level Percentage of deduplicated Percentage of total 1 71.44400432120995 49.6 2 18.83327331652863 26.150000000000002 3 6.049693914296003 12.6 4 2.3046453006841916 6.4 5 0.9002520705797623 3.125 6 0.25207057976233344 1.05 7 0.18005041411595246 0.8750000000000001 8 0.036010082823190494 0.2 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GCCAGACATATTGTAACCGAGTATCCTTTCTGAAGGATTGCCATTAGAAT 8 0.2 No Hit GCATTATGGTGGGGCTTGAAGACTATAGACAATGGTGTCCAAGTCTTTTT 7 0.17500000000000002 No Hit CCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAAT 7 0.17500000000000002 No Hit GCTCCACCACATCCACATCCACATCCTCCACCTCAGGAGCTGCTGCTATT 7 0.17500000000000002 No Hit TGAAGAAATGAAAGACAGTCGGTTACAGAAAGAAAGACATGTAAGAGAGC 7 0.17500000000000002 No Hit CAGCCGGGCTCACACTTCCCAACGTCCGAGCAGGAGTCTTTCCTCCTTTC 7 0.17500000000000002 No Hit CAACGTAGGTAGGTATATCACCCTTTCCAGGGGCTGTGATGAGCACCTTC 6 0.15 No Hit ATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATA 6 0.15 No Hit TGCGGGACAAGAGCTCGGGGAAGACGCATCCAAGAGCTCCAAGCATGGCC 6 0.15 No Hit CACCGCTGTCAATTTGCTCAAGTACACCATCTTTATCAAAAACCACCGAA 6 0.15 No Hit GCGTCTTTCTCAACATACCCTGTCCAACCGCGTTTACTCGGAGGGAATGT 6 0.15 No Hit CACAGATTCGTTCATGGCAAGATATTCTGGAGCCCGTCCTCCAGGTATCA 6 0.15 No Hit CACAAAATTCATCCCAGCCTGCTTTCTGTAAATAAATAGAGTTGCTGATC 6 0.15 No Hit TAAAAAAGTGTGGCATATACAACAGCTGGACTAAGATCATCATTGTTTTA 5 0.125 No Hit CGAGGCAGGCACGAAGCAATCAGGGGTCAAAGTTCCTGAGCCAAGCCTTG 5 0.125 No Hit ACCAAAAAAAAAGAAAAAAAAAAGAACCTAGATCTGGAAACTAGAAATTT 5 0.125 No Hit CTTTCAGTTTGTTGTCTTCTTGGTGTTTTCCTCGCGGAGAAAATCAAGCA 5 0.125 No Hit CGACGGAGTATTGAGGGGAGTGGCTCCATGTTTTCTCACAGAAGCAGTCC 5 0.125 No Hit GTTCCATCAAAACCATTTGTTTCTGCTCCCTTCAGAACAGAGTCTGATAT 5 0.125 No Hit CTGCTAGAGTCCACTGGTCCTCTGCCCCAGAAGCATAGTAGTCAAAGACC 5 0.125 No Hit TCCGCCCTTGCTGCTCTCATAACAGCAGCAGCCTGTTCTTCAAGTTGCTT 5 0.125 No Hit CACCAAAACCTCTACTTCCCATTATCACAGCACTTAACCCCAACCTCTCA 5 0.125 No Hit CATGATACAGTAGTTCTTCAGGTGAAGAAAGCGAATCCAGCTTCATTGTC 5 0.125 No Hit ACGCACAAATACATTGAACTTCAGCACATTTAAGTGTTTACTTGAAACTT 5 0.125 No Hit ATCAGAAATTGCAGCTGATATATCAATTATCAATTTCACTGATCTGTTGA 5 0.125 No Hit GTGGTTCCTGAAGAAGCATGCTCAATGATGACCATTTTCTAGATGCTGAG 5 0.125 No Hit GCCTTTCTCAATCTCTGCAATTTCTTCCCTAATGGAGATAAGCGCATCAC 5 0.125 No Hit GGCGATAATCCGAGTTAGGTCTTCTCGTGGCTACTGCTATAAACTTCTTG 5 0.125 No Hit GCATCGCCAAGATTGTTCCAGGCTGTCACATACCCTGGTTGAAGCTTCAC 5 0.125 No Hit CACGCATAGGTGGATATCTTTCTTTTGGATTGATGCCCAAATTGAAACAG 5 0.125 No Hit CATCGATCACATTGCCTTGACTTCGGAAGTGATTGCCCTGTTGGTGTTGG 5 0.125 No Hit GGGGGAAAAAAAAAAGGAAAAAAAAAAGGGACCCCAGACACAGCCAAGAT 5 0.125 No Hit CTCAGGATTATATGTGGGGGCTCCAAATATCGCATACCAATCACAGACAT 5 0.125 No Hit TGCTTGTGTTGTGCTGTTCTCACTGGGCAGGGATTCCAAGGATTATAAGG 5 0.125 No Hit CAATACCCCAACCATTGCAAGTCCTATTTGCTCTCTGACAATACCCTTTA 5 0.125 No Hit GCACTCTCAGAAGATGAGGATTTGCACATCCAGCAACAAGGCGGATGGCA 5 0.125 No Hit TCGGCATCAAATACTCTAACTTGAGCATCGTTGTTCGCGGCCATAATCCT 5 0.125 No Hit GTCTTCATGCCACATGTTTCCTGAAAATTCCCAATTAAAGTGAGGAGGAC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.07500000000000001 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.1 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.1375 0.0 0.0 0.0 0.0 82-83 0.16249999999999998 0.0 0.0 0.0 0.0 84-85 0.175 0.0 0.0 0.0 0.0 86-87 0.1875 0.0 0.0 0.0 0.0 88-89 0.30000000000000004 0.0 0.0 0.0 0.0 90-91 0.325 0.0 0.0 0.0 0.0 92-93 0.375 0.0 0.0 0.0 0.0 94-95 0.4375 0.0 0.0 0.0 0.0 96-97 0.48750000000000004 0.0 0.0 0.0 0.0 98-99 0.55 0.0 0.0 0.0 0.0 100-101 0.6 0.0 0.0 0.0 0.0 102-103 0.7875000000000001 0.0 0.0 0.0 0.0 104-105 0.8625 0.0 0.0 0.0 0.0 106-107 0.9375 0.0 0.0 0.0 0.0 108-109 1.05 0.0 0.0 0.0 0.0 110-111 1.2 0.0 0.0 0.0 0.0 112-113 1.3125 0.0 0.0 0.0 0.0 114-115 1.4625 0.0 0.0 0.0 0.0 116-117 1.6 0.0 0.0 0.0 0.0 118-119 1.85 0.0 0.0 0.0 0.0 120-121 2.0250000000000004 0.0 0.0 0.0 0.0 122-123 2.2249999999999996 0.0 0.0 0.0 0.0 124-125 2.65 0.0 0.0 0.0 0.0 126-127 2.9749999999999996 0.0 0.0 0.0 0.0 128-129 3.2625 0.0 0.0 0.0 0.0 130-131 3.75 0.0 0.0 0.0 0.0 132-133 3.95 0.0 0.0 0.0 0.0 134-135 4.25 0.0 0.0 0.0 0.0 136-137 4.7375 0.0 0.0 0.0 0.0 138-139 5.175000000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GAGGCGG 10 0.006830828 145.0 9 GAGTGAT 10 0.006830828 145.0 145 GATACAT 10 0.006830828 145.0 7 TACATTG 10 0.006830828 145.0 9 AAGACTT 10 0.006830828 145.0 2 GAGTAGC 10 0.006830828 145.0 145 GCAGATA 10 0.006830828 145.0 4 TCAGAAA 10 0.006830828 145.0 2 CAGATAC 10 0.006830828 145.0 5 TGAGGCG 10 0.006830828 145.0 8 TCACACA 10 0.006830828 145.0 8 AGATACA 10 0.006830828 145.0 6 >>END_MODULE SRR13695467 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695467_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.1105 37.0 37.0 37.0 37.0 37.0 2 36.089 37.0 37.0 37.0 37.0 37.0 3 36.124 37.0 37.0 37.0 37.0 37.0 4 36.233 37.0 37.0 37.0 37.0 37.0 5 36.143 37.0 37.0 37.0 37.0 37.0 6 36.303 37.0 37.0 37.0 37.0 37.0 7 36.187 37.0 37.0 37.0 37.0 37.0 8 36.264 37.0 37.0 37.0 37.0 37.0 9 36.2275 37.0 37.0 37.0 37.0 37.0 10-14 36.198 37.0 37.0 37.0 37.0 37.0 15-19 36.167500000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.1476 37.0 37.0 37.0 37.0 37.0 25-29 36.01835 37.0 37.0 37.0 37.0 37.0 30-34 35.9952 37.0 37.0 37.0 37.0 37.0 35-39 35.94225 37.0 37.0 37.0 37.0 37.0 40-44 35.9471 37.0 37.0 37.0 37.0 37.0 45-49 35.96105 37.0 37.0 37.0 37.0 37.0 50-54 35.9408 37.0 37.0 37.0 37.0 37.0 55-59 35.90045 37.0 37.0 37.0 37.0 37.0 60-64 35.897349999999996 37.0 37.0 37.0 37.0 37.0 65-69 35.834500000000006 37.0 37.0 37.0 37.0 37.0 70-74 35.81265 37.0 37.0 37.0 37.0 37.0 75-79 35.723349999999996 37.0 37.0 37.0 37.0 37.0 80-84 35.79615 37.0 37.0 37.0 37.0 37.0 85-89 35.7044 37.0 37.0 37.0 37.0 37.0 90-94 35.70945 37.0 37.0 37.0 37.0 37.0 95-99 35.7031 37.0 37.0 37.0 37.0 37.0 100-104 35.64425 37.0 37.0 37.0 37.0 37.0 105-109 35.6119 37.0 37.0 37.0 37.0 37.0 110-114 35.57084999999999 37.0 37.0 37.0 37.0 37.0 115-119 35.60405 37.0 37.0 37.0 37.0 37.0 120-124 35.48975 37.0 37.0 37.0 37.0 37.0 125-129 35.4864 37.0 37.0 37.0 37.0 37.0 130-134 35.3925 37.0 37.0 37.0 34.6 37.0 135-139 35.50765 37.0 37.0 37.0 37.0 37.0 140-144 35.39375 37.0 37.0 37.0 34.6 37.0 145-149 35.31385 37.0 37.0 37.0 34.6 37.0 150-151 35.017875000000004 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 3.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 1.0 14 0.0 15 2.0 16 3.0 17 0.0 18 1.0 19 1.0 20 1.0 21 1.0 22 5.0 23 8.0 24 6.0 25 9.0 26 10.0 27 12.0 28 14.0 29 24.0 30 35.0 31 47.0 32 66.0 33 106.0 34 217.0 35 613.0 36 2607.0 37 208.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 29.191767068273094 19.126506024096386 19.126506024096386 32.55522088353413 2 27.925 25.174999999999997 30.599999999999998 16.3 3 22.15 26.8 30.0 21.05 4 23.200000000000003 34.300000000000004 23.75 18.75 5 25.75 35.025 22.375 16.85 6 20.225 38.275 23.35 18.15 7 20.875 21.625 39.375 18.125 8 22.15 26.674999999999997 27.325 23.849999999999998 9 22.175 26.875 28.849999999999998 22.1 10-14 23.28 29.330000000000002 26.224999999999998 21.165 15-19 23.145 28.575 27.365000000000002 20.915 20-24 22.553042433947155 28.262610088070456 27.692153722978386 21.492193755004003 25-29 23.058823529411764 28.300375469336668 27.92991239048811 20.710888610763455 30-34 22.217217217217218 27.842842842842842 28.723723723723726 21.216216216216218 35-39 22.904468798478707 27.823650102587198 27.753590551969175 21.51829054696492 40-44 21.866866866866864 28.163163163163162 28.98898898898899 20.98098098098098 45-49 22.09098643711526 27.581202142034932 29.337870977428558 20.98994044342125 50-54 22.573058446757404 28.848078462770214 27.111689351481182 21.467173738991193 55-59 22.377972465581976 27.76971214017522 28.445556946182727 21.406758448060074 60-64 22.95451133463444 26.67267177100535 28.914577390782163 21.458239503578042 65-69 23.12656328164082 27.668834417208604 27.658829414707352 21.54577288644322 70-74 23.44430538172716 28.075093867334168 27.544430538172715 20.93617021276596 75-79 22.41353421092147 27.67405776064868 28.11452024625857 21.79788778217128 80-84 23.947960970728047 27.390542907180386 27.36052039029272 21.30097573179885 85-89 24.20905086103324 27.93352022426912 27.017420905086105 20.840008009611534 90-94 23.009558124405746 27.788620327278185 27.838662863433917 21.36315868488215 95-99 22.788230584467577 28.14251401120897 27.937349879903923 21.131905524419537 100-104 24.14518147684606 27.899874843554446 26.84355444305382 21.111389236545683 105-109 22.63215859030837 28.464156988386062 27.75330396475771 21.150380456547857 110-114 24.22559175299004 27.263173697642994 27.97878196466997 20.53245258469699 115-119 23.436952495369674 28.48776092506382 27.526655654002102 20.5486309255644 120-124 23.819774718397998 27.614518147684606 27.879849812265334 20.685857321652065 125-129 23.896285914505956 28.56642306537191 26.904595054560016 20.632695965562117 130-134 24.546910984279563 28.552117753079003 26.499449284069286 20.40152197857214 135-139 24.430538172715895 28.01501877346683 27.50438047559449 20.05006257822278 140-144 25.14137016463994 28.294049942451082 26.77275684331682 19.791823049592153 145-149 24.81602002503129 27.704630788485606 27.32916145181477 20.150187734668336 150-151 25.973213168106145 27.888346476405058 26.32369508073601 19.814745274752784 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.5 8 0.5 9 0.5 10 0.5 11 0.0 12 1.0 13 1.0 14 0.5 15 1.0 16 1.0 17 1.0 18 1.5 19 1.5 20 2.0 21 2.5 22 1.5 23 1.5 24 1.0 25 7.0 26 13.5 27 14.0 28 12.0 29 9.5 30 22.0 31 33.5 32 34.5 33 43.5 34 52.5 35 57.5 36 79.0 37 112.0 38 129.5 39 151.0 40 201.5 41 237.0 42 237.5 43 261.0 44 279.0 45 278.5 46 263.5 47 216.0 48 208.5 49 194.0 50 151.5 51 125.5 52 112.5 53 100.5 54 79.5 55 62.0 56 44.0 57 33.0 58 28.0 59 25.5 60 20.0 61 12.5 62 9.0 63 5.5 64 3.0 65 1.5 66 1.0 67 1.0 68 0.0 69 0.0 70 0.0 71 0.0 72 0.5 73 0.5 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.5 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 1.0 95 1.5 96 2.0 97 1.5 98 0.0 99 0.0 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.08 25-29 0.125 30-34 0.1 35-39 0.08499999999999999 40-44 0.1 45-49 0.095 50-54 0.08 55-59 0.125 60-64 0.08499999999999999 65-69 0.05 70-74 0.125 75-79 0.105 80-84 0.075 85-89 0.12 90-94 0.08499999999999999 95-99 0.08 100-104 0.125 105-109 0.12 110-114 0.08499999999999999 115-119 0.11499999999999999 120-124 0.125 125-129 0.11 130-134 0.13 135-139 0.125 140-144 0.08499999999999999 145-149 0.125 150-151 0.13749999999999998 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.69999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 72.20229555236729 50.324999999999996 2 18.579626972740314 25.900000000000002 3 5.272596843615495 11.025 4 2.582496413199426 7.199999999999999 5 0.860832137733142 3.0 6 0.2152080344332855 0.8999999999999999 7 0.1793400286944046 0.8750000000000001 8 0.03586800573888092 0.2 9 0.0 0.0 >10 0.07173601147776185 0.575 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CAGAGAACACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 13 0.325 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 10 0.25 No Hit GATTGTGCTGCTCGAGGTGCTGATCTTTTCGAAAGTAGTGCTGCTGAAAC 8 0.2 No Hit TTAACCTGTCTGCTTTGCTTTGCTTGGCATTTTCCCCCCAAATAGAGCCA 7 0.17500000000000002 No Hit GCCTATAAATTGTAGTAGTTGAGCACCATTATAGACATCTCCAAGCTCAT 7 0.17500000000000002 No Hit TAGGGATAATGGGAAAGGCCATGTCCATGAATTTACTTAAAAATGGCTTC 7 0.17500000000000002 No Hit ATGGGTATGTCACAAAATAAGTGTACTATCAGACCACTTGTTACTGCTCT 7 0.17500000000000002 No Hit GTAATTAACTGTAAGTATGCTAATTGCCATTTTCTTTATTTCATGGTGAG 7 0.17500000000000002 No Hit AGCTCAAAAAAAATCTTAACTGCACCCGCTCATCCAGCAATGGCAGCAGC 6 0.15 No Hit CACACCTTCTCAAGTATGACTCCACCCTTGGCATCTTCGCAGCTGATGTT 6 0.15 No Hit CAAAAAAAATCTTAACTGCACCCGCTCATCCAGCAATGGCAGCAGCAACA 6 0.15 No Hit TTCCATTTCAAGCTCAATTAATGCTAAAGCCATGACAGTTGAGGGACAAA 6 0.15 No Hit TGTGGACTTGAGAGGCGTTAAGAACACCGCTGGAATTGAGCCTGAAGATG 6 0.15 No Hit GTTCCTGAGTGCTAACTCGAGTGCTGTTCTTTTTGAGGTCATCTCTGCTA 6 0.15 No Hit CGAGAGTGCATATGCAGTCAAATGGGCGGTCCAGAACTACCTCCGTCCAG 5 0.125 No Hit TGGTGGAAGGGTTTTTGAACTTAGCCCTGAGCAGTATGTCCTCAAAGTTG 5 0.125 No Hit GTGCATTAAATTTATCACAAGACCATCTTTTGTTCAAGAGAGAAGTATTA 5 0.125 No Hit GCAAATTCAAGTAAAAATGTCTTCATAATTCTGGCATCTTCAGTTTGATT 5 0.125 No Hit CGAGTCCACGTTATACTTCCACAGAAGAAGCGTAGAAGATAACTGGATAA 5 0.125 No Hit CCTGGATAAAGCCTTTTCTGCTGGGATTCACATTCTTCTGACTTCTGGTG 5 0.125 No Hit TCTTTGCCTGCAATATCAATGAAACCGTCATCAAGGAAACAGCTGATGCA 5 0.125 No Hit ATCATCACCATCGGATTCAACAACATTATCATTTCATAAAACCTAACGAA 5 0.125 No Hit CTTGGTCCTCATGGTTGCCTTGGACAAAATCTGGTTCAGCCCACTTGGTA 5 0.125 No Hit AGAACACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATC 5 0.125 No Hit TTCAGACAGCCCACATTACGCTGATGGGGTGCATTCCTCACTCTTAGAGG 5 0.125 No Hit GTCAAAACTTTGTATGGGGTGGTTTTCCAGGCCCGGAGAAGGAGTTTGAA 5 0.125 No Hit TTCACGTGGGTTGATTAAGATTCCAGGTTTCAATTTAGGTGCATTCAAAT 5 0.125 No Hit AAGTAAGCTTAGTGAAGAGCATCTGAAGCAGGCTTTGGATGCAGAGTCAA 5 0.125 No Hit CTCATGTGTTTCATCGGAGCCTGAAGACATGAGTACTGTCAACTACCCCA 5 0.125 No Hit GTACAATCCAAGCCAGCTGCTCCCTTGGTGAACCCTGCCCCCCCTTCCTT 5 0.125 No Hit CTAAAGAATTGCAGGAGCAAGTAAGAAGCGGTGATGCCTCTGCAACTGGA 5 0.125 No Hit TGATAGCTCCGATACCTCTCTCAAATTAGGGTTGGCCATCTGATGCTGAA 5 0.125 No Hit GTTTGGCGAAGGAAGTTTAGGTGCTATTACTGTTAGGCACCCATGATGAA 5 0.125 No Hit GTAAGAGGGGGGATTTAACCGTTGATTTTAAAGATGGAGATAACCAATGT 5 0.125 No Hit CTTTTCTTCTATTCACAGTAATTCTTTCACTTCAAGAGATGTATCGCTCT 5 0.125 No Hit CAGAGAGCATGCAGAAGATTTTACCATTTTCTTTTCTGAAAAAATCCACA 5 0.125 No Hit CAGAGACCGTAGAGTTGTCTGCCTGGTATTTAGCAGGATTAACTACGCAT 5 0.125 No Hit GCTGAGGAATCTGCTGTGGGCAACATCAAAGCATGATGTTTACCTTATGC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.07500000000000001 0.0 0.0 0.0 0.0 70-71 0.1 0.0 0.0 0.0 0.0 72-73 0.1 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.125 0.0 0.0 0.0 0.0 78-79 0.125 0.0 0.0 0.0 0.0 80-81 0.1375 0.0 0.0 0.0 0.0 82-83 0.16249999999999998 0.0 0.0 0.0 0.0 84-85 0.175 0.0 0.0 0.0 0.0 86-87 0.1875 0.0 0.0 0.0 0.0 88-89 0.30000000000000004 0.0 0.0 0.0 0.0 90-91 0.325 0.0 0.0 0.0 0.0 92-93 0.375 0.0 0.0 0.0 0.0 94-95 0.4375 0.0 0.0 0.0 0.0 96-97 0.48750000000000004 0.0 0.0 0.0 0.0 98-99 0.55 0.0 0.0 0.0 0.0 100-101 0.6 0.0 0.0 0.0 0.0 102-103 0.7875000000000001 0.0 0.0 0.0 0.0 104-105 0.8625 0.0 0.0 0.0 0.0 106-107 0.9375 0.0 0.0 0.0 0.0 108-109 1.05 0.0 0.0 0.0 0.0 110-111 1.2 0.0 0.0 0.0 0.0 112-113 1.2875 0.0 0.0 0.0 0.0 114-115 1.4249999999999998 0.0 0.0 0.0 0.0 116-117 1.55 0.0 0.0 0.0 0.0 118-119 1.825 0.0 0.0 0.0 0.0 120-121 2.0 0.0 0.0 0.0 0.0 122-123 2.2 0.0 0.0 0.0 0.0 124-125 2.625 0.0 0.0 0.0 0.0 126-127 2.9625 0.0 0.0 0.0 0.0 128-129 3.2625 0.0 0.0 0.0 0.0 130-131 3.75 0.0 0.0 0.0 0.0 132-133 3.95 0.0 0.0 0.0 0.0 134-135 4.25 0.0 0.0 0.0 0.0 136-137 4.7375 0.0 0.0 0.0 0.0 138-139 5.175000000000001 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAACTTT 10 0.006830828 145.0 5 CTGAGGT 10 0.006830828 145.0 1 CTTTAAA 10 0.006830828 145.0 6 TAAAATT 10 0.006830828 145.0 9 TAAAAGG 10 0.006830828 145.0 2 AAGAGAA 10 0.006830828 145.0 4 TGACACT 10 0.006830828 145.0 1 AACTTTG 10 0.006830828 145.0 6 ACTTTAA 10 0.006830828 145.0 5 ATGTACT 10 0.006830828 145.0 145 TTAAAAT 10 0.006830828 145.0 8 TTTAAAA 10 0.006830828 145.0 7 GTAAAAG 10 0.006830828 145.0 1 >>END_MODULE Read 1002055 spots for SRR13695467.sra Written 1002055 spots for SRR13695467.sra Read 1002055 spots for SRR13695467.sra Written 1002055 spots for SRR13695467.sra Read 1002055 spots for SRR13695467.sra Written 1002055 spots for SRR13695467.sra Read 1002055 spots for SRR13695467.sra Written 1002055 spots for SRR13695467.sra Read 1002055 spots for SRR13695467.sra Written 1002055 spots for SRR13695467.sra Read 1002055 spots for SRR13695467.sra Written 1002055 spots for SRR13695467.sra Read 1002055 spots for SRR13695467.sra Written 1002055 spots for SRR13695467.sra Read 1002055 spots for SRR13695467.sra Written 1002055 spots for SRR13695467.sra Read 1002055 spots for SRR13695467.sra Written 1002055 spots for SRR13695467.sra Read 1002055 spots for SRR13695467.sra Written 1002055 spots for SRR13695467.sra Read 1002055 spots for SRR13695467.sra Written 1002055 spots for SRR13695467.sra Read 1002055 spots for SRR13695467.sra Written 1002055 spots for SRR13695467.sra Read 1002055 spots for SRR13695467.sra Written 1002055 spots for SRR13695467.sra Read 1002055 spots for SRR13695467.sra Written 1002055 spots for SRR13695467.sra Read 1002055 spots for SRR13695467.sra Written 1002055 spots for SRR13695467.sra Read 1002055 spots for SRR13695467.sra Written 1002055 spots for SRR13695467.sra Read 1002055 spots for SRR13695467.sra Written 1002055 spots for SRR13695467.sra Read 1002055 spots for SRR13695467.sra Written 1002055 spots for SRR13695467.sra Read 1002055 spots for SRR13695467.sra Written 1002055 spots for SRR13695467.sra Read 1002072 spots for SRR13695467.sra Written 1002072 spots for SRR13695467.sra SRR ids: ['SRR13695467.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_1_t7l9oi SRR13695467.sra spots: 20041117 blocks: [[1, 1002055], [1002056, 2004110], [2004111, 3006165], [3006166, 4008220], [4008221, 5010275], [5010276, 6012330], [6012331, 7014385], [7014386, 8016440], [8016441, 9018495], [9018496, 10020550], [10020551, 11022605], [11022606, 12024660], [12024661, 13026715], [13026716, 14028770], [14028771, 15030825], [15030826, 16032880], [16032881, 17034935], [17034936, 18036990], [18036991, 19039045], [19039046, 20041117]] SRR13695467 file size 6789147 SRR13695467 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695467 SRR13695467_1.fastq SRR13695467_2.fastq Input file: SRR13695467_1.fastq Paired file: SRR13695467_2.fastq trimmed: SRR13695467-trimmed-pair1.fastq, SRR13695467-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 05:08:02 2025 >> started Wed Feb 12 05:08:23 2025 >> done (21.370s) 20041117 read pairs processed; of these: 121 ( 0.00%) short read pairs filtered out after trimming by size control 43660 ( 0.22%) empty read pairs filtered out after trimming by size control 19997336 (99.78%) read pairs available; of these: 1472119 ( 7.36%) trimmed read pairs available after processing 18525217 (92.64%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 0 0.00% 20 2 0.00% 21 1 0.00% 22 1 0.00% 23 2 0.00% 24 1 0.00% 25 3 0.00% 26 0 0.00% 27 0 0.00% 28 4 0.00% 29 1 0.00% 30 4 0.00% 31 6 0.00% 32 5 0.00% 33 6 0.00% 34 5 0.00% 35 4 0.00% 36 9 0.00% 37 9 0.00% 38 14 0.00% 39 8 0.00% 40 18 0.00% 41 17 0.00% 42 12 0.00% 43 22 0.00% 44 12 0.00% 45 23 0.00% 46 38 0.00% 47 30 0.00% 48 38 0.00% 49 50 0.00% 50 68 0.00% 51 53 0.00% 52 64 0.00% 53 75 0.00% 54 53 0.00% 55 88 0.00% 56 130 0.00% 57 103 0.00% 58 144 0.00% 59 155 0.00% 60 184 0.00% 61 217 0.00% 62 251 0.00% 63 261 0.00% 64 324 0.00% 65 278 0.00% 66 401 0.00% 67 378 0.00% 68 476 0.00% 69 505 0.00% 70 611 0.00% 71 748 0.00% 72 784 0.00% 73 892 0.00% 74 1080 0.01% 75 1204 0.01% 76 1283 0.01% 77 1390 0.01% 78 1412 0.01% 79 1786 0.01% 80 1907 0.01% 81 2231 0.01% 82 2618 0.01% 83 2770 0.01% 84 3052 0.02% 85 3477 0.02% 86 3814 0.02% 87 3826 0.02% 88 4158 0.02% 89 4545 0.02% 90 5011 0.03% 91 5287 0.03% 92 5678 0.03% 93 6136 0.03% 94 6901 0.03% 95 7526 0.04% 96 7593 0.04% 97 8334 0.04% 98 8748 0.04% 99 8910 0.04% 100 9699 0.05% 101 9826 0.05% 102 10589 0.05% 103 11462 0.06% 104 11882 0.06% 105 12583 0.06% 106 13106 0.07% 107 13614 0.07% 108 13925 0.07% 109 14701 0.07% 110 14851 0.07% 111 15980 0.08% 112 16206 0.08% 113 17060 0.09% 114 17648 0.09% 115 18475 0.09% 116 19473 0.10% 117 20059 0.10% 118 21137 0.11% 119 20895 0.10% 120 21688 0.11% 121 22396 0.11% 122 23328 0.12% 123 23854 0.12% 124 25025 0.13% 125 25572 0.13% 126 26978 0.13% 127 27892 0.14% 128 27966 0.14% 129 28706 0.14% 130 29463 0.15% 131 30235 0.15% 132 30990 0.15% 133 32085 0.16% 134 32499 0.16% 135 33624 0.17% 136 34187 0.17% 137 35696 0.18% 138 36032 0.18% 139 37672 0.19% 140 37969 0.19% 141 38829 0.19% 142 39228 0.20% 143 39596 0.20% 144 41357 0.21% 145 42459 0.21% 146 42687 0.21% 147 44053 0.22% 148 45451 0.23% 149 46146 0.23% 150 47019 0.24% 151 18525217 92.64% 19997336 reads passed initial QC criterion=sequence-density sequence-density=0.46 sequence-density-rank=1 fanout-score=2.07 fanout-score-rank=29 prefix-density=0.49 prefix-fanout=1.9 sequence=TTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGGGTATGAATGTGTTCTCTG criterion=fanout-score sequence-density=0.01 sequence-density-rank=34 fanout-score=17.80 fanout-score-rank=1 prefix-density=0.04 prefix-fanout=3.2 sequence=GACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTG criterion=sequence-density sequence-density=0.51 sequence-density-rank=1 fanout-score=2.22 fanout-score-rank=31 prefix-density=0.53 prefix-fanout=2.2 sequence=TGTAAGAGATGGCTTCCTC criterion=fanout-score sequence-density=0.17 sequence-density-rank=15 fanout-score=28.59 fanout-score-rank=1 prefix-density=0.43 prefix-fanout=11.1 sequence=AAAGAAAAGAAAA SRR13695467 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 05:09:02 Started mapping on | Feb 12 05:09:02 Finished on | Feb 12 05:11:12 Mapping speed, Million of reads per hour | 553.77 Number of input reads | 19997336 Average input read length | 298 UNIQUE READS: Uniquely mapped reads number | 18659927 Uniquely mapped reads % | 93.31% Average mapped length | 297.15 Number of splices: Total | 18245639 Number of splices: Annotated (sjdb) | 17859234 Number of splices: GT/AG | 17878349 Number of splices: GC/AG | 294109 Number of splices: AT/AC | 10440 Number of splices: Non-canonical | 62741 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.03% Deletion average length | 2.91 Insertion rate per base | 0.02% Insertion average length | 2.39 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 446375 % of reads mapped to multiple loci | 2.23% Number of reads mapped to too many loci | 85082 % of reads mapped to too many loci | 0.43% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.79% % of reads unmapped: other | 0.24% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 891291 891291 891291 N_multimapping 446375 446375 446375 N_noFeature 788511 18239246 1035561 N_ambiguous 287358 1922 112340 UnstrandedReadsAssigned:17584058 PositiveStrandReadsAssigned:418759 NegativeStrandReadsAssigned:17512026 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695467 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695467-trimmed-pair1.fastq SRR13695467-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 19,997,336 reads, 17,577,240 reads pseudoaligned [quant] estimated average fragment length: 270.506 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,234 rounds 52401 SRR13695467.ke.tsv 34699 SRR13695467.se.tsv 87100 total ==> SRR13695467.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1748.49 581.52 16.9756 Potri.005G024800.1.v4.1 1035 765.494 476 31.7387 Potri.004G059700.1.v4.1 961 691.679 0 0 Potri.007G009000.2.v4.1 1416 1146.49 0 0 Potri.003G141000.2.v4.1 2943 2673.49 1195.1 22.8166 Potri.016G087400.1.v4.1 270 76.8192 995.52 661.46 Potri.015G069301.1.v4.1 564 306.63 0 0 Potri.010G195200.1.v4.1 1773 1503.49 147 4.99044 Potri.012G127500.1.v4.1 977 707.601 111 8.00679 ==> SRR13695467.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 404 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 314 Potri.001G212900.v4.1 68 Potri.001G182400.v4.1 1 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 5 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 0 SRR13695467 completed mapping pipeline successfully