Starting /dee2/code/volunteer_pipeline.sh SRR13695468 current disk space = 3049127084032 free memory = 875322636 SRR13695468 SRAfilesize 59a1b79523c4d272d166df598d474ba6 SRR13695468.sra SRR13695468.sra file validated SRR13695468 is paired end SRR13695468 is conventional basespace SRR13695468 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695468_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5105 37.0 37.0 37.0 37.0 37.0 2 36.31625 37.0 37.0 37.0 37.0 37.0 3 36.557 37.0 37.0 37.0 37.0 37.0 4 36.4915 37.0 37.0 37.0 37.0 37.0 5 36.5125 37.0 37.0 37.0 37.0 37.0 6 36.574 37.0 37.0 37.0 37.0 37.0 7 36.443 37.0 37.0 37.0 37.0 37.0 8 36.547 37.0 37.0 37.0 37.0 37.0 9 36.573 37.0 37.0 37.0 37.0 37.0 10-14 36.5154 37.0 37.0 37.0 37.0 37.0 15-19 36.5197 37.0 37.0 37.0 37.0 37.0 20-24 36.5054 37.0 37.0 37.0 37.0 37.0 25-29 36.4517 37.0 37.0 37.0 37.0 37.0 30-34 36.425200000000004 37.0 37.0 37.0 37.0 37.0 35-39 36.3743 37.0 37.0 37.0 37.0 37.0 40-44 36.3745 37.0 37.0 37.0 37.0 37.0 45-49 36.378499999999995 37.0 37.0 37.0 37.0 37.0 50-54 36.345600000000005 37.0 37.0 37.0 37.0 37.0 55-59 36.307900000000004 37.0 37.0 37.0 37.0 37.0 60-64 36.2978 37.0 37.0 37.0 37.0 37.0 65-69 36.293899999999994 37.0 37.0 37.0 37.0 37.0 70-74 36.2562 37.0 37.0 37.0 37.0 37.0 75-79 36.208600000000004 37.0 37.0 37.0 37.0 37.0 80-84 36.1524 37.0 37.0 37.0 37.0 37.0 85-89 36.1955 37.0 37.0 37.0 37.0 37.0 90-94 36.0475 37.0 37.0 37.0 37.0 37.0 95-99 36.0506 37.0 37.0 37.0 37.0 37.0 100-104 36.0298 37.0 37.0 37.0 37.0 37.0 105-109 36.0612 37.0 37.0 37.0 37.0 37.0 110-114 36.001799999999996 37.0 37.0 37.0 37.0 37.0 115-119 35.9748 37.0 37.0 37.0 37.0 37.0 120-124 35.944900000000004 37.0 37.0 37.0 37.0 37.0 125-129 35.836499999999994 37.0 37.0 37.0 37.0 37.0 130-134 35.868399999999994 37.0 37.0 37.0 37.0 37.0 135-139 35.7937 37.0 37.0 37.0 37.0 37.0 140-144 35.7069 37.0 37.0 37.0 37.0 37.0 145-149 35.579699999999995 37.0 37.0 37.0 37.0 37.0 150-151 35.5295 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 1.0 21 0.0 22 0.0 23 2.0 24 0.0 25 4.0 26 6.0 27 13.0 28 15.0 29 30.0 30 30.0 31 30.0 32 55.0 33 75.0 34 131.0 35 335.0 36 2937.0 37 335.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.175 10.85 8.325000000000001 42.65 2 19.84465046354297 14.056627411676272 35.95590077674768 30.14282134803307 3 18.35 18.325 28.549999999999997 34.775 4 24.525 23.1 23.65 28.725 5 23.200000000000003 32.025 23.0 21.775 6 20.525 35.35 23.25 20.875 7 16.125 27.125 40.225 16.525000000000002 8 19.125 24.525 33.525 22.825 9 18.375 25.424999999999997 32.775 23.425 10-14 20.1 29.42 27.779999999999998 22.7 15-19 19.885 28.18 27.875 24.060000000000002 20-24 20.169999999999998 28.410000000000004 27.12 24.3 25-29 20.035 28.035 27.605 24.325 30-34 20.810000000000002 28.189999999999998 27.74 23.26 35-39 20.105 29.020000000000003 27.115000000000002 23.76 40-44 20.165 28.79 27.084999999999997 23.96 45-49 19.685 28.355000000000004 28.144999999999996 23.815 50-54 20.205000000000002 27.735 28.305000000000003 23.755000000000003 55-59 21.145 27.325 27.54 23.990000000000002 60-64 20.165 27.905 28.044999999999998 23.885 65-69 20.84 28.26 27.495000000000005 23.405 70-74 20.125 28.225 27.925 23.724999999999998 75-79 20.125 28.910000000000004 27.095000000000002 23.87 80-84 21.2 27.76 27.365000000000002 23.674999999999997 85-89 20.635 28.544999999999998 27.935 22.884999999999998 90-94 20.72 28.449999999999996 27.51 23.32 95-99 21.48 29.015 26.474999999999998 23.03 100-104 20.95 28.64 28.249999999999996 22.16 105-109 21.235 27.66 28.349999999999998 22.755 110-114 21.855 27.48 27.565 23.1 115-119 21.185000000000002 27.950000000000003 27.650000000000002 23.215 120-124 20.724999999999998 28.375 27.37 23.53 125-129 21.135 27.860000000000003 27.42 23.585 130-134 20.565 28.610000000000003 27.275 23.549999999999997 135-139 21.445 28.93 26.305 23.32 140-144 22.03 27.500000000000004 26.46 24.01 145-149 21.44 27.994999999999997 26.495 24.07 150-151 22.375 28.762500000000003 24.637500000000003 24.224999999999998 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.0 20 1.5 21 1.5 22 1.0 23 0.5 24 1.0 25 2.0 26 4.0 27 8.0 28 13.5 29 18.5 30 18.5 31 20.0 32 30.0 33 37.5 34 58.0 35 78.5 36 88.5 37 97.5 38 119.0 39 145.5 40 159.5 41 193.5 42 232.0 43 244.5 44 244.0 45 273.5 46 281.0 47 250.5 48 242.5 49 234.0 50 176.0 51 136.5 52 125.0 53 95.0 54 81.5 55 74.5 56 59.5 57 37.5 58 32.5 59 29.5 60 22.5 61 15.5 62 5.0 63 2.5 64 1.0 65 1.5 66 2.0 67 1.0 68 0.5 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.22499999999999998 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.3 #Duplication Level Percentage of deduplicated Percentage of total 1 68.43891402714932 45.375 2 19.570135746606336 25.95 3 7.239819004524888 14.399999999999999 4 3.205128205128205 8.5 5 0.904977375565611 3.0 6 0.3770739064856712 1.5 7 0.1885369532428356 0.8750000000000001 8 0.07541478129713425 0.4 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCTCATCCTCTGCACTAGGAAGCACTCTCACAAGTTCCATATCTGAAACT 8 0.2 No Hit GGAGAAGAAAGGAGCAAAGAAGATATGGTGAGACTGACAGCCGACTTGAT 8 0.2 No Hit GTCGGATAACTTCCTCGACAGCTGCCTCTGCACTTGCAACCAGTTGCTCT 7 0.17500000000000002 No Hit CCTCCCCATTAAGTCCAAATCCTCCACAGCTCCCTCTATAGCCTTTTGCA 7 0.17500000000000002 No Hit GTTTTCTTGGTGACCCATCATCAGGTCTTGAAGCTTTCACAAATGGGCAA 7 0.17500000000000002 No Hit GTTGCAAAACCTTGGAGGAGGAGGAGTAGGAGACGGAAGCAGAGTTGATG 7 0.17500000000000002 No Hit ACTCTCCAATCACCTTATTGCTATTATTACTACTAATCCCGTTACTCTTC 7 0.17500000000000002 No Hit GTCGTTAAGAGACCACCAATATTCCACAGAATATGGAGGTATGCCCCAGC 6 0.15 No Hit CCAATCAGAATCAGCCCATCCTGATGATATATTATCTCCAACGCCGACAA 6 0.15 No Hit AACGGAAACACTCTGCACCAATGGTGATAACCTGGCCATCAGGTAGCTCA 6 0.15 No Hit CCCGAATTCAGATGAAATGGCAACACTTTTTAGAAACCGAGAGGACAAAG 6 0.15 No Hit CACCATCCTCATTTTTAACTTGTAATTTCTGTTCAAGTACCCTAGTAGAA 6 0.15 No Hit AGCAGAGGAACCAGCTTTCCTTCCTGGTGCATGCCTGTGGTTTTGTCACA 6 0.15 No Hit GGGAAATGGAGATGGATACATTGGGAAAGTTGTTAGTGGTTCAATCTCCC 6 0.15 No Hit CCTGTGATCCCCAAATTTCGGACGTGAGCTGACTAAAGAACCAGTAAAAC 6 0.15 No Hit CCAGCAACCACCTGTTCTTTGGCCTTCACCACCCTCACAAACTCAAGAAG 6 0.15 No Hit CGGGAGGTCAATCTCTTCTGCCTCCTCAGCATGGTCAAGGATGTAGATGT 6 0.15 No Hit GATCCTTTTACTTCCATGTATGTCTTGCTCTGGATTTCTTCATAGGTCAG 5 0.125 No Hit CAAGAACTAAAAGAAGCTTCAACAGGATCAGGGATTTTGTCTTCCCTTGT 5 0.125 No Hit ACGGATAGTTCCAGGTTCAGAAGCCGCAGGATTTGTGGCTCCGATAATCT 5 0.125 No Hit AGGGCGAGGTACTGGACGAGGATAATGATTCTCATTTTGCATGAACTGGT 5 0.125 No Hit GCCACTTGTTCCCCTGTACATTGAAGTTTACTTGTTTACATCGATACATA 5 0.125 No Hit CTTGCTTTTACCGCTACATCAGGAGTTACTCCATTCACTGCCTCACGTAA 5 0.125 No Hit CGTGCGTGGATCAAGCTTGGGTTGCCCAAGTAGTCAAGTCCACCCTCGCT 5 0.125 No Hit TCTGCTATTTCAGCTATTTGTTGTGCAGCAAAAGCAGGCTCACATGAGCT 5 0.125 No Hit AGCACACTTAACCTTCACAGTGTCAAAGAAGGGAAGGCCCTGGACTTCAA 5 0.125 No Hit TGAGGAATAATAATAGCGCACCCAAACTGCCACGCTAAAAAAAAAAAAAA 5 0.125 No Hit CCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCC 5 0.125 No Hit GTATCGGTCTTGATCTTCTTGACACCACTGGCTTCAATCTTGAAAGTCCT 5 0.125 No Hit GCTCTCTGAGGACATAGTTACAAAGCCAAAACCACGTGAACGGCCAGTCT 5 0.125 No Hit CTCGAGATTCTCAGGGACCTCACCCAGTCTAAGTGGGTCGAACCCAAAGT 5 0.125 No Hit GCTCCACCATCCAAAACTCAGCCAAATGCCTTGAAGTGTGAGAATGCTCA 5 0.125 No Hit GACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTG 5 0.125 No Hit ATCAGGATCCGTAACATTAAAAACTGGTGGGAAAAAAAACTCTAAATAAT 5 0.125 No Hit CAGAGAAGGAGTTGGGGAGAGTTAGGGTTTTAGAGAGAGGAGTGGCCATG 5 0.125 No Hit CTCAGGTGTTACCTGTACAGAATCAAGTTAGCCCTTTTGAGATTAATTAA 5 0.125 No Hit AGGCGGTTGAACCATGTTGATTTCCCTGCGTTTGTATAGCCGACAAGCGC 5 0.125 No Hit TGCTACTGTTTTTAATGCCATAGCCGCCGGGTACGGCGGCCCACGTGGGG 5 0.125 No Hit CACTATACAAGGTTTACACAAGTGAAGCACAATCGATTAAAGAAATGGTG 5 0.125 No Hit CTAAAGCTAAGGTTTCACCCTCCAAACCTAAAGAGAAAAGGTGAGCGCTT 5 0.125 No Hit CCTCATCTGCCTTCCTCACCAAAGCCTTAACAGTGTAACCCCTGAGCATA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0375 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.1125 0.0 0.0 0.0 0.0 78-79 0.2 0.0 0.0 0.0 0.0 80-81 0.21250000000000002 0.0 0.0 0.0 0.0 82-83 0.25 0.0 0.0 0.0 0.0 84-85 0.32499999999999996 0.0 0.0 0.0 0.0 86-87 0.4125 0.0 0.0 0.0 0.0 88-89 0.475 0.0 0.0 0.0 0.0 90-91 0.6125 0.0 0.0 0.0 0.0 92-93 0.675 0.0 0.0 0.0 0.0 94-95 0.8 0.0 0.0 0.0 0.0 96-97 1.0375 0.0 0.0 0.0 0.0 98-99 1.1375 0.0 0.0 0.0 0.0 100-101 1.4625 0.0 0.0 0.0 0.0 102-103 1.7 0.0 0.0 0.0 0.0 104-105 2.1625 0.0 0.0 0.0 0.0 106-107 2.4 0.0 0.0 0.0 0.0 108-109 2.925 0.0 0.0 0.0 0.0 110-111 3.25 0.0 0.0 0.0 0.0 112-113 3.4875 0.0 0.0 0.0 0.0 114-115 3.6375 0.0 0.0 0.0 0.0 116-117 3.9250000000000003 0.0 0.0 0.0 0.0 118-119 4.3625 0.0 0.0 0.0 0.0 120-121 4.675000000000001 0.0 0.0 0.0 0.0 122-123 5.15 0.0 0.0 0.0 0.0 124-125 5.575 0.0 0.0 0.0 0.0 126-127 5.95 0.0 0.0 0.0 0.0 128-129 6.4125 0.0 0.0 0.0 0.0 130-131 7.237500000000001 0.0 0.0 0.0 0.0 132-133 7.7875 0.0 0.0 0.0 0.0 134-135 8.2875 0.0 0.0 0.0 0.0 136-137 9.075 0.0 0.0 0.0 0.0 138-139 9.9375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTAGAA 10 0.006830828 145.0 5 >>END_MODULE SRR13695468 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695468_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.06075 37.0 37.0 37.0 37.0 37.0 2 36.09 37.0 37.0 37.0 37.0 37.0 3 36.1655 37.0 37.0 37.0 37.0 37.0 4 36.29 37.0 37.0 37.0 37.0 37.0 5 36.24 37.0 37.0 37.0 37.0 37.0 6 36.1835 37.0 37.0 37.0 37.0 37.0 7 36.193 37.0 37.0 37.0 37.0 37.0 8 36.193 37.0 37.0 37.0 37.0 37.0 9 36.224 37.0 37.0 37.0 37.0 37.0 10-14 36.2625 37.0 37.0 37.0 37.0 37.0 15-19 36.2178 37.0 37.0 37.0 37.0 37.0 20-24 36.16199999999999 37.0 37.0 37.0 37.0 37.0 25-29 36.1736 37.0 37.0 37.0 37.0 37.0 30-34 36.186299999999996 37.0 37.0 37.0 37.0 37.0 35-39 36.1394 37.0 37.0 37.0 37.0 37.0 40-44 36.1271 37.0 37.0 37.0 37.0 37.0 45-49 36.0863 37.0 37.0 37.0 37.0 37.0 50-54 36.0575 37.0 37.0 37.0 37.0 37.0 55-59 36.0707 37.0 37.0 37.0 37.0 37.0 60-64 36.0721 37.0 37.0 37.0 37.0 37.0 65-69 35.966 37.0 37.0 37.0 37.0 37.0 70-74 35.94760000000001 37.0 37.0 37.0 37.0 37.0 75-79 35.938 37.0 37.0 37.0 37.0 37.0 80-84 35.9465 37.0 37.0 37.0 37.0 37.0 85-89 35.8524 37.0 37.0 37.0 37.0 37.0 90-94 35.8538 37.0 37.0 37.0 37.0 37.0 95-99 35.851 37.0 37.0 37.0 37.0 37.0 100-104 35.83570000000001 37.0 37.0 37.0 37.0 37.0 105-109 35.7487 37.0 37.0 37.0 37.0 37.0 110-114 35.70360000000001 37.0 37.0 37.0 37.0 37.0 115-119 35.6437 37.0 37.0 37.0 37.0 37.0 120-124 35.604499999999994 37.0 37.0 37.0 37.0 37.0 125-129 35.5947 37.0 37.0 37.0 37.0 37.0 130-134 35.455 37.0 37.0 37.0 34.6 37.0 135-139 35.48945 37.0 37.0 37.0 34.6 37.0 140-144 35.3075 37.0 37.0 37.0 34.6 37.0 145-149 35.15845 37.0 37.0 37.0 27.4 37.0 150-151 34.7485 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 2.0 14 0.0 15 0.0 16 1.0 17 0.0 18 1.0 19 0.0 20 1.0 21 4.0 22 1.0 23 4.0 24 9.0 25 8.0 26 10.0 27 11.0 28 11.0 29 23.0 30 33.0 31 35.0 32 70.0 33 97.0 34 213.0 35 622.0 36 2645.0 37 198.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.922403003754695 20.425531914893615 12.11514392991239 29.536921151439298 2 27.675 26.025 30.475 15.825 3 20.875 28.849999999999998 31.05 19.225 4 25.025 34.55 22.8 17.625 5 26.85 35.5 21.075 16.575 6 21.45 38.1 22.475 17.974999999999998 7 20.7 20.9 39.1 19.3 8 19.75 26.450000000000003 29.725 24.075 9 20.3 26.0 29.849999999999998 23.849999999999998 10-14 22.895 29.404999999999998 26.590000000000003 21.11 15-19 22.595000000000002 29.14 27.165 21.099999999999998 20-24 22.939999999999998 28.875 27.195000000000004 20.990000000000002 25-29 22.39 29.349999999999998 27.965 20.294999999999998 30-34 21.975 28.255000000000003 28.444999999999997 21.325 35-39 22.685 28.65 27.985 20.68 40-44 22.935 28.075 28.29 20.7 45-49 21.965 29.615000000000002 27.060000000000002 21.36 50-54 22.685 28.549999999999997 28.355000000000004 20.41 55-59 22.830000000000002 27.76 28.02 21.39 60-64 22.98 27.685 27.82 21.515 65-69 22.935 27.495000000000005 27.26 22.31 70-74 23.465 27.49 28.235 20.810000000000002 75-79 22.71 28.044999999999998 28.199999999999996 21.044999999999998 80-84 22.915 28.365000000000002 26.875 21.845 85-89 23.565 28.4 27.74 20.294999999999998 90-94 22.689999999999998 28.09 27.500000000000004 21.72 95-99 23.715 27.82 27.49 20.974999999999998 100-104 23.925 27.63 27.284999999999997 21.16 105-109 23.56 28.52 27.095000000000002 20.825 110-114 23.575 28.305000000000003 27.065 21.055 115-119 23.400000000000002 28.34 27.275 20.985 120-124 23.87 27.150000000000002 28.26 20.72 125-129 25.314999999999998 27.634999999999998 26.505000000000003 20.544999999999998 130-134 25.217521752175216 27.527752775277527 27.122712271227122 20.13201320132013 135-139 25.351267563378173 28.07140357017851 26.936346817340866 19.640982049102455 140-144 25.629999999999995 27.389999999999997 26.36 20.62 145-149 25.336266813340668 27.651382569128458 27.326366318315916 19.68598429921496 150-151 27.51937984496124 26.906726681670417 26.644161040260066 18.929732433108278 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 1.0 6 1.0 7 0.5 8 0.5 9 0.5 10 0.0 11 0.0 12 0.5 13 1.0 14 1.0 15 1.0 16 1.0 17 1.0 18 1.5 19 2.0 20 1.5 21 0.5 22 1.5 23 3.5 24 2.0 25 2.5 26 6.0 27 7.5 28 15.0 29 22.0 30 19.0 31 21.0 32 30.0 33 50.5 34 51.5 35 45.0 36 75.5 37 118.5 38 154.0 39 180.0 40 198.0 41 214.5 42 238.0 43 248.0 44 250.0 45 265.5 46 267.5 47 235.0 48 201.0 49 185.0 50 180.5 51 154.0 52 118.5 53 95.5 54 78.5 55 70.5 56 52.5 57 34.5 58 23.5 59 20.0 60 18.5 61 13.5 62 8.0 63 3.0 64 0.5 65 0.0 66 1.0 67 1.0 68 0.0 69 0.0 70 0.5 71 1.0 72 0.5 73 0.5 74 0.5 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.125 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.01 135-139 0.005 140-144 0.0 145-149 0.005 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.14999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 68.36734693877551 45.225 2 19.690098261526835 26.05 3 7.029478458049887 13.950000000000001 4 3.2879818594104306 8.7 5 0.9070294784580499 3.0 6 0.45351473922902497 1.7999999999999998 7 0.1889644746787604 0.8750000000000001 8 0.07558578987150416 0.4 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTAGTTTTGGCAAGTACTCTCCAATGTTGGGATTGATTCGGGTAATTCTG 8 0.2 No Hit GGTCGACGCCACGTATCCCTTCTAAAAAGCATTGTCATTGATGGGGAAAC 8 0.2 No Hit TACATCGACTGCGCGTGAAACACCACGCGCCCAACAAGGCCTGTCTTTGA 7 0.17500000000000002 No Hit CCAGAAGAAATATTCATAAGCTTTGGTGTCACTTGTAACTCTGATTTTGA 7 0.17500000000000002 No Hit AGTAAAGATGCTGCAGGACAAGTTCGTGGTTCTCCTAATGAATTTGCTGG 7 0.17500000000000002 No Hit GGGAGGTGAAAAAAGGTGAGAAAGTTGGATTAGTTGGTGTAAATGGAGCA 7 0.17500000000000002 No Hit TGCTGGATTCTGGTGATGGAGTTAGTCACACAGTTCCCATATATGAGGGC 7 0.17500000000000002 No Hit ATTGACTTTTTCTATCCACCAAACAATGGACGCCTTCGCTTCCTTCTTTG 6 0.15 No Hit CTGCATAGCTTATAAGCTTGTAAGAGATGGCTTCCTCCTCTATGATCTCA 6 0.15 No Hit TGGTCAGCACATCGTTTACTCGCCGGGTGCCAGTGACAAGCCTACGGGCA 6 0.15 No Hit TCTATCTCTCTCTCTCAAACCCAAACAAACAGAAAAATGGATCTGTTCAA 6 0.15 No Hit AGAGAATCGAATAAATCTCTTTTGTTTGACGAAGACGACGACGAAGACGA 6 0.15 No Hit AGGCGAAGGAGCTGGTATCCACCAATCCCGTGGTGGTTTTCAGCAAGACA 6 0.15 No Hit CAAACCCACTTCGCTCTCTTTTTTATCCCTCTCCTCCACTTCTTCTATCA 6 0.15 No Hit CATGGGTACGATAACTGGCATAAGTGACTTAGATGTTGCTAGATGGCCAA 6 0.15 No Hit GAAAGAGATTCATAACTAATTCATCAATGGAATCGTCATGGGTGAACACT 6 0.15 No Hit AGAAGACGACCATGACGGACGCGTGGATTCGAGGTGTAGTGGAAGCCATA 6 0.15 No Hit CATGCATCTAGGGGTTCTTGCTACTGCTTCTCATGCCATTGCGACTGGAA 6 0.15 No Hit CTTCGATTAACAGCTGGTGTCTCACCTCTGTCTCTGCCTCTAAGAGATCA 6 0.15 No Hit CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT 5 0.125 No Hit CTTGTTTTTATTTCTAAGAACTAAAATGGCAGCCTCACTGCAAGCAGCAG 5 0.125 No Hit GGCAAAACAAGTGCCTGAGCAGAATGGTTTGACTCACTTTGAGGCAGGAG 5 0.125 No Hit CTCAAAAAAAATCTTAACTGCACCCGCTCATCCAGCAATGGCAGCAGCAA 5 0.125 No Hit AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 5 0.125 No Hit CTGCAACAGAAGAGGAAAAGACTCAAGGGAGAGAAATGGAGCAAACCTTC 5 0.125 No Hit ACCAGGCAGAGAGAGAGAGATGAACAAAACGCATTCAAAGCTGTTGAAGA 5 0.125 No Hit CTCAGGGTTGGGACAGATATCAAGAACAACACGTGCAGATGACAAGGAAG 5 0.125 No Hit TTTTAGTATTCGCTGTTTCTTTCTTTTTTGAAATGGCTTCAACTGCTACT 5 0.125 No Hit GTGAGATTGTTGCTCAACTCAAAGCTGCTAAGGCAACCAGGGACGATATT 5 0.125 No Hit GTGGGCAACCTACCATGGGGAGTGGATAGTGGTCGTCTTGAGGAAGTCTT 5 0.125 No Hit CAACGCCAGCCACCCAGCTGCGACATCCGAAATCGACATGGTAAAAAAAC 5 0.125 No Hit GTGATACTTCTTTTCCTTTTTTCGACACTTCTTTTCCTTTTTTCTTTCTT 5 0.125 No Hit TCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAATCATCATGT 5 0.125 No Hit GCATATTCGCAGGGACAAGGCTACCAGCAACATCTGTACTGCTCAAGCAT 5 0.125 No Hit ATCATAAATTAAGTTAGCCATTGGTTGAATTCTTCTATGAGTGTTACTTT 5 0.125 No Hit CTCACATCAACATCGTGGTCATTGGCCATGTGGACTCTGGAAAGTCAACC 5 0.125 No Hit AGGGATCAACCTTTCCCGGCAAGGCGGAGGAATTGGGGCAAGAGGTCCCG 5 0.125 No Hit GTCTGGTGCCTATTGTGGAGCCTGAGATTTTGACTGATGGATCTCATGAC 5 0.125 No Hit CAGCAATAGCTAAGCCGCACCATCAGCTAAACAATGGCAGCAGCAACAAT 5 0.125 No Hit AAGCTAGATCACCAAAAAGTACTAAAATCCTAACAAAAGCAAGAATCAAT 5 0.125 No Hit GGATTTTCAAGCTAAGTCTTCAGATTCTCAATCTATTGGAAACTCTTCTC 5 0.125 No Hit GGACCGTTCGACAACCTCTTGCAGCACATCTCAGACCCGTGGCACAACAC 5 0.125 No Hit TTCAAAGTCCAAATTTGCCTCTTCAATTCCACTTCCTAGTGTCAATGGCA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0375 0.0 0.0 0.0 0.0 68-69 0.075 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.075 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.1125 0.0 0.0 0.0 0.0 78-79 0.2 0.0 0.0 0.0 0.0 80-81 0.21250000000000002 0.0 0.0 0.0 0.0 82-83 0.25 0.0 0.0 0.0 0.0 84-85 0.32499999999999996 0.0 0.0 0.0 0.0 86-87 0.4125 0.0 0.0 0.0 0.0 88-89 0.475 0.0 0.0 0.0 0.0 90-91 0.5875 0.0 0.0 0.0 0.0 92-93 0.65 0.0 0.0 0.0 0.0 94-95 0.775 0.0 0.0 0.0 0.0 96-97 1.0125 0.0 0.0 0.0 0.0 98-99 1.1125 0.0 0.0 0.0 0.0 100-101 1.4375 0.0 0.0 0.0 0.0 102-103 1.7 0.0 0.0 0.0 0.0 104-105 2.1625 0.0 0.0 0.0 0.0 106-107 2.4124999999999996 0.0 0.0 0.0 0.0 108-109 2.95 0.0 0.0 0.0 0.0 110-111 3.275 0.0 0.0 0.0 0.0 112-113 3.5125 0.0 0.0 0.0 0.0 114-115 3.6624999999999996 0.0 0.0 0.0 0.0 116-117 3.95 0.0 0.0 0.0 0.0 118-119 4.3875 0.0 0.0 0.0 0.0 120-121 4.699999999999999 0.0 0.0 0.0 0.0 122-123 5.1875 0.0 0.0 0.0 0.0 124-125 5.6625 0.0 0.0 0.0 0.0 126-127 6.075 0.0 0.0 0.0 0.0 128-129 6.5375 0.0 0.0 0.0 0.0 130-131 7.35 0.0 0.0 0.0 0.0 132-133 7.8875 0.0 0.0 0.0 0.0 134-135 8.4125 0.0 0.0 0.0 0.0 136-137 9.225 0.0 0.0 0.0 0.0 138-139 10.1125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGCAATC 10 0.006830828 145.0 2 GCTTATA 10 0.006830828 145.0 2 >>END_MODULE Read 743385 spots for SRR13695468.sra Written 743385 spots for SRR13695468.sra Read 743385 spots for SRR13695468.sra Written 743385 spots for SRR13695468.sra Read 743385 spots for SRR13695468.sra Written 743385 spots for SRR13695468.sra Read 743385 spots for SRR13695468.sra Written 743385 spots for SRR13695468.sra Read 743385 spots for SRR13695468.sra Written 743385 spots for SRR13695468.sra Read 743385 spots for SRR13695468.sra Written 743385 spots for SRR13695468.sra Read 743385 spots for SRR13695468.sra Written 743385 spots for SRR13695468.sra Read 743385 spots for SRR13695468.sra Written 743385 spots for SRR13695468.sra Read 743386 spots for SRR13695468.sra Written 743386 spots for SRR13695468.sra Read 743385 spots for SRR13695468.sra Written 743385 spots for SRR13695468.sra Read 743385 spots for SRR13695468.sra Written 743385 spots for SRR13695468.sra Read 743385 spots for SRR13695468.sra Written 743385 spots for SRR13695468.sra Read 743385 spots for SRR13695468.sra Written 743385 spots for SRR13695468.sra Read 743385 spots for SRR13695468.sra Written 743385 spots for SRR13695468.sra Read 743385 spots for SRR13695468.sra Written 743385 spots for SRR13695468.sra Read 743385 spots for SRR13695468.sra Written 743385 spots for SRR13695468.sra Read 743385 spots for SRR13695468.sra Written 743385 spots for SRR13695468.sra Read 743385 spots for SRR13695468.sra Written 743385 spots for SRR13695468.sra Read 743385 spots for SRR13695468.sra Written 743385 spots for SRR13695468.sra Read 743385 spots for SRR13695468.sra Written 743385 spots for SRR13695468.sra SRR ids: ['SRR13695468.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_egmcu562 SRR13695468.sra spots: 14867701 blocks: [[1, 743385], [743386, 1486770], [1486771, 2230155], [2230156, 2973540], [2973541, 3716925], [3716926, 4460310], [4460311, 5203695], [5203696, 5947080], [5947081, 6690465], [6690466, 7433850], [7433851, 8177235], [8177236, 8920620], [8920621, 9664005], [9664006, 10407390], [10407391, 11150775], [11150776, 11894160], [11894161, 12637545], [12637546, 13380930], [13380931, 14124315], [14124316, 14867701]] SRR13695468 file size 5030994 SRR13695468 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695468 SRR13695468_1.fastq SRR13695468_2.fastq Input file: SRR13695468_1.fastq Paired file: SRR13695468_2.fastq trimmed: SRR13695468-trimmed-pair1.fastq, SRR13695468-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 04:53:41 2025 >> started Wed Feb 12 04:53:58 2025 >> done (16.790s) 14867701 read pairs processed; of these: 96 ( 0.00%) short read pairs filtered out after trimming by size control 3121 ( 0.02%) empty read pairs filtered out after trimming by size control 14864484 (99.98%) read pairs available; of these: 1931518 (12.99%) trimmed read pairs available after processing 12932966 (87.01%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 5 0.00% 19 1 0.00% 20 3 0.00% 21 2 0.00% 22 3 0.00% 23 5 0.00% 24 1 0.00% 25 4 0.00% 26 0 0.00% 27 1 0.00% 28 4 0.00% 29 1 0.00% 30 5 0.00% 31 0 0.00% 32 3 0.00% 33 6 0.00% 34 4 0.00% 35 10 0.00% 36 7 0.00% 37 6 0.00% 38 11 0.00% 39 20 0.00% 40 16 0.00% 41 11 0.00% 42 9 0.00% 43 31 0.00% 44 18 0.00% 45 16 0.00% 46 39 0.00% 47 33 0.00% 48 59 0.00% 49 55 0.00% 50 69 0.00% 51 69 0.00% 52 87 0.00% 53 112 0.00% 54 108 0.00% 55 116 0.00% 56 135 0.00% 57 152 0.00% 58 179 0.00% 59 253 0.00% 60 269 0.00% 61 357 0.00% 62 370 0.00% 63 452 0.00% 64 482 0.00% 65 532 0.00% 66 584 0.00% 67 625 0.00% 68 720 0.00% 69 887 0.01% 70 999 0.01% 71 1152 0.01% 72 1279 0.01% 73 1525 0.01% 74 1722 0.01% 75 1825 0.01% 76 2117 0.01% 77 2352 0.02% 78 2663 0.02% 79 2984 0.02% 80 3278 0.02% 81 3742 0.03% 82 4027 0.03% 83 4535 0.03% 84 5154 0.03% 85 5676 0.04% 86 6173 0.04% 87 6448 0.04% 88 7264 0.05% 89 7436 0.05% 90 8141 0.05% 91 8685 0.06% 92 9255 0.06% 93 10159 0.07% 94 10972 0.07% 95 11597 0.08% 96 12302 0.08% 97 12555 0.08% 98 13367 0.09% 99 14270 0.10% 100 15022 0.10% 101 15623 0.11% 102 16345 0.11% 103 17261 0.12% 104 18080 0.12% 105 18821 0.13% 106 19605 0.13% 107 20291 0.14% 108 20925 0.14% 109 22042 0.15% 110 22519 0.15% 111 23105 0.16% 112 24016 0.16% 113 24166 0.16% 114 25472 0.17% 115 26446 0.18% 116 27318 0.18% 117 28256 0.19% 118 29341 0.20% 119 29388 0.20% 120 30779 0.21% 121 31725 0.21% 122 32394 0.22% 123 32907 0.22% 124 33355 0.22% 125 34352 0.23% 126 35243 0.24% 127 36297 0.24% 128 37274 0.25% 129 37922 0.26% 130 38625 0.26% 131 39071 0.26% 132 40108 0.27% 133 40287 0.27% 134 40904 0.28% 135 42181 0.28% 136 42293 0.28% 137 43332 0.29% 138 43977 0.30% 139 45577 0.31% 140 45311 0.30% 141 46776 0.31% 142 46872 0.32% 143 47608 0.32% 144 48459 0.33% 145 49406 0.33% 146 48646 0.33% 147 50108 0.34% 148 51204 0.34% 149 51712 0.35% 150 52170 0.35% 151 12932966 87.01% 14864484 reads passed initial QC criterion=sequence-density sequence-density=0.40 sequence-density-rank=1 fanout-score=2.42 fanout-score-rank=28 prefix-density=0.44 prefix-fanout=2.2 sequence=TGAGATGCCTCAGTGCATCCAAACATGGGTAGCTTCCACATAGTCCAGTAGCGTCCATCATAGTACCCTGGTGAGCTGTGGTGCTCACGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTTCGAGCGAAGAAGGTAATCAATTTCCTTGGCCAATTCCTCCTCTGTGAGATCTGGAAGGTAAGAAAGAGTCTCGAACTTCTTCAATCCAGTTGGTGGCCACACCTGCATGCATTGAACTCGTCCACCATTGCTTGCAATGGAAGTAATGTCATTAGCCTTTCTGGTACTGACTGGGAAAGCTGCGGCAGACTTGAGACCATTGAATGGTGCCACCATGTTGGCTTGTGCCGGGGTGCGGTTGACGGTGGCAACGGCTGCCGATGAGATCATAGAGGAGGAAGCCAT criterion=fanout-score sequence-density=0.01 sequence-density-rank=38 fanout-score=398.91 fanout-score-rank=1 prefix-density=0.28 prefix-fanout=17.1 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGAGTATGAATGTGTTCTCTGTG criterion=sequence-density sequence-density=0.65 sequence-density-rank=1 fanout-score=2.09 fanout-score-rank=28 prefix-density=0.66 prefix-fanout=2.1 sequence=TGTAAGAGATGGCTTCCTC criterion=fanout-score sequence-density=0.04 sequence-density-rank=31 fanout-score=188.67 fanout-score-rank=1 prefix-density=0.32 prefix-fanout=23.4 sequence=AGAGAGAGAGACAGAGAGAATGGCCACCACAGCAGCCCTCTCCAGCGCCATGGTCAGCACATCGTTTACTCGCCGGGTGCCAGTGACAAGCCTACGGGCACTTCCCAACGTGGGGGAGTCTCTTCTTGGCTTGAAAGCCAGTCGAGGAGGACGTGTTAAAGCAATGGCAG SRR13695468 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 04:54:44 Started mapping on | Feb 12 04:54:44 Finished on | Feb 12 04:56:38 Mapping speed, Million of reads per hour | 469.40 Number of input reads | 14864484 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 13847603 Uniquely mapped reads % | 93.16% Average mapped length | 294.10 Number of splices: Total | 13551675 Number of splices: Annotated (sjdb) | 13274766 Number of splices: GT/AG | 13278572 Number of splices: GC/AG | 220319 Number of splices: AT/AC | 6963 Number of splices: Non-canonical | 45821 Mismatch rate per base, % | 0.38% Deletion rate per base | 0.03% Deletion average length | 2.89 Insertion rate per base | 0.02% Insertion average length | 2.42 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 322078 % of reads mapped to multiple loci | 2.17% Number of reads mapped to too many loci | 96230 % of reads mapped to too many loci | 0.65% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.86% % of reads unmapped: other | 0.17% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 694961 694961 694961 N_multimapping 322078 322078 322078 N_noFeature 562936 13488301 791044 N_ambiguous 209326 1446 77087 UnstrandedReadsAssigned:13075341 PositiveStrandReadsAssigned:357856 NegativeStrandReadsAssigned:12979472 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695468 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695468-trimmed-pair1.fastq SRR13695468-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 14,864,484 reads, 13,038,540 reads pseudoaligned [quant] estimated average fragment length: 246.39 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,224 rounds 52401 SRR13695468.ke.tsv 34699 SRR13695468.se.tsv 87100 total ==> SRR13695468.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1772.61 383 14.7908 Potri.005G024800.1.v4.1 1035 789.61 153 13.2643 Potri.004G059700.1.v4.1 961 715.719 3 0.286936 Potri.007G009000.2.v4.1 1416 1170.61 0 0 Potri.003G141000.2.v4.1 2943 2697.61 656.479 16.6589 Potri.016G087400.1.v4.1 270 87.5841 694 542.425 Potri.015G069301.1.v4.1 564 327.983 0 0 Potri.010G195200.1.v4.1 1773 1527.61 54 2.41984 Potri.012G127500.1.v4.1 977 731.671 57 5.33291 ==> SRR13695468.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 34 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 200 Potri.001G212900.v4.1 1 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 1 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 6 SRR13695468 completed mapping pipeline successfully