Starting /dee2/code/volunteer_pipeline.sh SRR13695469 current disk space = 3048984805376 free memory = 1559102604 SRR13695469 SRAfilesize 952e4b6a5f817c5ac4f4d7201b4d0801 SRR13695469.sra SRR13695469.sra file validated SRR13695469 is paired end SRR13695469 is conventional basespace SRR13695469 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695469_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5395 37.0 37.0 37.0 37.0 37.0 2 36.3185 37.0 37.0 37.0 37.0 37.0 3 36.5555 37.0 37.0 37.0 37.0 37.0 4 36.5585 37.0 37.0 37.0 37.0 37.0 5 36.502 37.0 37.0 37.0 37.0 37.0 6 36.5465 37.0 37.0 37.0 37.0 37.0 7 36.4795 37.0 37.0 37.0 37.0 37.0 8 36.613 37.0 37.0 37.0 37.0 37.0 9 36.509 37.0 37.0 37.0 37.0 37.0 10-14 36.5475 37.0 37.0 37.0 37.0 37.0 15-19 36.4941 37.0 37.0 37.0 37.0 37.0 20-24 36.5332 37.0 37.0 37.0 37.0 37.0 25-29 36.4351 37.0 37.0 37.0 37.0 37.0 30-34 36.38699999999999 37.0 37.0 37.0 37.0 37.0 35-39 36.3724 37.0 37.0 37.0 37.0 37.0 40-44 36.364900000000006 37.0 37.0 37.0 37.0 37.0 45-49 36.3362 37.0 37.0 37.0 37.0 37.0 50-54 36.3036 37.0 37.0 37.0 37.0 37.0 55-59 36.2926 37.0 37.0 37.0 37.0 37.0 60-64 36.2829 37.0 37.0 37.0 37.0 37.0 65-69 36.229499999999994 37.0 37.0 37.0 37.0 37.0 70-74 36.235200000000006 37.0 37.0 37.0 37.0 37.0 75-79 36.27720000000001 37.0 37.0 37.0 37.0 37.0 80-84 36.2325 37.0 37.0 37.0 37.0 37.0 85-89 36.1543 37.0 37.0 37.0 37.0 37.0 90-94 36.1352 37.0 37.0 37.0 37.0 37.0 95-99 36.084 37.0 37.0 37.0 37.0 37.0 100-104 36.0712 37.0 37.0 37.0 37.0 37.0 105-109 36.1032 37.0 37.0 37.0 37.0 37.0 110-114 36.0699 37.0 37.0 37.0 37.0 37.0 115-119 36.048500000000004 37.0 37.0 37.0 37.0 37.0 120-124 35.9968 37.0 37.0 37.0 37.0 37.0 125-129 36.0177 37.0 37.0 37.0 37.0 37.0 130-134 36.0175 37.0 37.0 37.0 37.0 37.0 135-139 35.857099999999996 37.0 37.0 37.0 37.0 37.0 140-144 35.834500000000006 37.0 37.0 37.0 37.0 37.0 145-149 35.643100000000004 37.0 37.0 37.0 37.0 37.0 150-151 35.4555 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 0.0 21 1.0 22 0.0 23 3.0 24 2.0 25 7.0 26 7.0 27 12.0 28 14.0 29 25.0 30 18.0 31 40.0 32 50.0 33 82.0 34 105.0 35 313.0 36 2944.0 37 376.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.475 12.575 8.924999999999999 38.025 2 21.20603015075377 13.115577889447236 33.994974874371856 31.683417085427134 3 18.575 16.175 27.3 37.95 4 23.075000000000003 22.275 24.45 30.2 5 23.849999999999998 29.599999999999998 24.175 22.375 6 21.25 31.75 24.8 22.2 7 16.475 27.075 39.35 17.1 8 20.175 28.199999999999996 29.975 21.65 9 16.75 26.950000000000003 35.725 20.575 10-14 19.53 29.785 27.76 22.925 15-19 20.255000000000003 28.125 27.54 24.08 20-24 20.535 28.08 27.655 23.73 25-29 19.295 29.53 27.675 23.5 30-34 20.365 29.15 27.195000000000004 23.29 35-39 21.349999999999998 28.87 26.669999999999998 23.11 40-44 21.12 29.435 26.540000000000003 22.905 45-49 20.86 29.765000000000004 25.900000000000002 23.474999999999998 50-54 20.515 29.270000000000003 27.155 23.06 55-59 20.49 27.57 27.87 24.07 60-64 20.674999999999997 29.235 26.334999999999997 23.755000000000003 65-69 20.655 28.349999999999998 26.924999999999997 24.07 70-74 20.78 28.720000000000002 26.47 24.03 75-79 20.555 27.955000000000002 27.845 23.645 80-84 20.085 28.384999999999998 27.900000000000002 23.630000000000003 85-89 20.369999999999997 28.655 26.695 24.279999999999998 90-94 21.395 27.66 27.29 23.655 95-99 20.895 27.839999999999996 27.785 23.48 100-104 20.68 28.4 26.815 24.104999999999997 105-109 21.245 27.85 27.395000000000003 23.51 110-114 20.919999999999998 28.095 27.485 23.5 115-119 21.5 28.12 26.784999999999997 23.595 120-124 20.645 28.994999999999997 27.1 23.26 125-129 21.58 27.66 26.805 23.955000000000002 130-134 20.755000000000003 28.305000000000003 26.465 24.474999999999998 135-139 21.83 28.645 25.955000000000002 23.57 140-144 21.584999999999997 28.57 25.705 24.14 145-149 20.995 28.349999999999998 26.235000000000003 24.42 150-151 21.85 28.799999999999997 25.324999999999996 24.025 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.5 2 0.5 3 0.0 4 0.5 5 0.5 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 0.5 21 1.0 22 0.5 23 0.0 24 0.5 25 1.5 26 5.0 27 8.0 28 5.0 29 13.5 30 25.0 31 28.5 32 31.0 33 43.5 34 65.0 35 84.0 36 108.5 37 108.5 38 110.5 39 133.5 40 168.0 41 203.5 42 216.0 43 225.0 44 262.5 45 257.0 46 214.0 47 208.5 48 212.5 49 226.0 50 203.5 51 166.0 52 145.0 53 114.5 54 84.0 55 73.5 56 65.5 57 49.0 58 37.0 59 26.5 60 19.5 61 17.5 62 10.0 63 2.0 64 3.5 65 3.5 66 2.5 67 3.0 68 2.5 69 0.5 70 0.5 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.85 #Duplication Level Percentage of deduplicated Percentage of total 1 67.99544419134396 44.775 2 20.007593014426728 26.35 3 7.137433561123766 14.099999999999998 4 3.1511009870918754 8.3 5 0.9491268033409264 3.125 6 0.45558086560364464 1.7999999999999998 7 0.15186028853454822 0.7000000000000001 8 0.07593014426727411 0.4 9 0.07593014426727411 0.44999999999999996 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TTCCCCAGATGCCTTCCGTGCATCTACCATGAAAAGTGATGGAGCAACTT 9 0.22499999999999998 No Hit CTCCATTTCATACAGTTCTTTTCATGACAAATTTTCACAGCACTTGCCAT 9 0.22499999999999998 No Hit CTCCAGTGTATCATAATTTTCTATACAAATTAGCCAGGGATGATAAGCTT 8 0.2 No Hit GACCATACAGCCTCGCCGAACTTGACACCGTTGCGGGACAAGAGCTCGGG 8 0.2 No Hit GGCTTCATATAGTCCTCAAACACAAATTTCGGGTAGATTTGGCTTTCCTT 7 0.17500000000000002 No Hit AGCAGTAATCCGCTTCTCAGGGTCATAGGTTAGAAGCTTGTTCAACAAAT 7 0.17500000000000002 No Hit AAGGGCTGCTAACCTCCTTTACAAAATATCAATTAATACATTAAAAAGAA 7 0.17500000000000002 No Hit ATCAAGTTCTGGGACGACTTAGACACCTTTCGGCTTGGCGGCAATAAAGC 7 0.17500000000000002 No Hit TCCGAACATTGAGAACATAGCCAACCTACCATTCTTGAGTTCCTTCACCT 6 0.15 No Hit CTCCGATACAGGCTGCCTCTAGTTTCTCAGTGAAATCCAAGAGTCTGGCA 6 0.15 No Hit GCTGAAGTTGGGACTGTGGATCCAGCGCCAGAGCCATTCAAGGCCAGCGG 6 0.15 No Hit ATCCGCTTTGATATTCTCTGCATCCTATTTAGGGCTATTGATATTTAACA 6 0.15 No Hit TTTTTTTTTTACCCTAACATGTCTTGTATTATTAGGAAAGAAAGTGACCC 6 0.15 No Hit CTTTCTCATTAGCAGCTAACCTCATCCTTGCAGCAGCATTAATCTCATTC 6 0.15 No Hit GTTAATATAAATTCAGAGTTTTGGAGCACTAACTAGACATAAAGGAAAAT 6 0.15 No Hit AGGGCGTTAGTAGCGCATAAAGCTGTAATCCATCCACTTTAATTATCAAA 6 0.15 No Hit GATATTCTTTGGTATAGTTGGGATTTTAATATCATTAATAGCATGATGGT 6 0.15 No Hit CTCCACTCAATGGATGTGATAGGGCGTTTATGGTAATCAAAATGTGCCAC 6 0.15 No Hit GGGCTATCGGATAAAACTCAAACAAACAAACCGATTTCATAATTCAGTCA 6 0.15 No Hit GACCACCAAAACGACCACCACTGCCACCACCTCTGCCACTATCCCGACTG 6 0.15 No Hit TCACCGTGTATGCTGCCATTGCTTTAACACGTCCTCCTCGACTGGCTTTC 5 0.125 No Hit TGCGGACATCTTGCTCAGCCCAACTTATCCTTGGACTGCCGCAAGTTCTT 5 0.125 No Hit GTTGCCGACCTCATAGAAGAAGACCCGAAAGGCCTACTGTTTCTAAAAAG 5 0.125 No Hit CTCTACTGTGCCAAAGCACTTGTGCTGTAAACAAGAAGTGCACCTCCGGC 5 0.125 No Hit GGGGAAGGTAACCCTTGGTTGAAAGATGGTTAATTGATACATAGATGAAC 5 0.125 No Hit CTCTTTGACAAGAAGTCTAGACAAGAACACACCAATGAATCTAGTGCCTC 5 0.125 No Hit GCACCGCCAACAAGGATAACCTCAGCAACGACAGCAAGGACGAGGTTGAT 5 0.125 No Hit GCCTCCGATCCTGCATATCGCTACTCCATTGTCTGTCAAATACTGAGAAG 5 0.125 No Hit GCCCGATTCAGCATCCGAATCCAGAAGCTAAAAACAAAAACAAAGTAGAA 5 0.125 No Hit CCCCAGGAAGCATCGTGTCTTCTGGTAGATCACAGAATGCAACACCCTCT 5 0.125 No Hit CTCACAGTATGTAACAGATGATTAAAGCTCGTCAGCAACAGCCTCCAGCC 5 0.125 No Hit GTCTCCAAGTCCTTCCAGGGGAGGTCAAGAGGGTTGCGGCTAGAAACGAC 5 0.125 No Hit ATCGGAATCGGAGACTCTTGGATGTCCTTCAGAAGTTTCTCTTCAGGAAC 5 0.125 No Hit GTCACGTTCACGGTGTGTCTCTCTCCTATAAGGACCATCACGATAGGAAT 5 0.125 No Hit CGATGAAGTCTTTTGACGGTGAAGTGGAGAGGGTGGAGAGAGTTGATTTC 5 0.125 No Hit GTTGCATATATCAAGGATGTCATCCCAATGCTCGTATGCAAAGTTCTCCT 5 0.125 No Hit AACAGGGGACTGTGTTTTGTTCGAATTTGTGGAACTGTTCCCACCGACAT 5 0.125 No Hit GTCTGCAGAATATCCCTTATAGGAGAGCTAGTTTCACACAACAAAATGGA 5 0.125 No Hit CCTGGCAATTTCTCCTTGATTTTGTCAAGGAAACCCTTCTTATCCTCTGG 5 0.125 No Hit GCCGGGGGCTGAGCCATCGAGGTAAGGTGGACGAGGCTGGCCTGGCATCC 5 0.125 No Hit CTTGTATGCATTTTTTGGACCAACTCCACCATCCACTTCAATCCATGGGT 5 0.125 No Hit GGCTTCTATATTTTAAACTCGTGTAGTCTGTCGTGGAACTTTCGTGTTGC 5 0.125 No Hit CCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATA 5 0.125 No Hit CTACAAAAGCAAGAAAAAGAAAGAGCAAAATCAAACAGCGCACATACTCC 5 0.125 No Hit GTTGCTTCAACCTCGGATTGCCCACCAGACAAAAACATGATTCCAGGGAC 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0125 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.025 0.0 0.0 0.0 0.0 84-85 0.05 0.0 0.0 0.0 0.0 86-87 0.07500000000000001 0.0 0.0 0.0 0.0 88-89 0.125 0.0 0.0 0.0 0.0 90-91 0.1875 0.0 0.0 0.0 0.0 92-93 0.3375 0.0 0.0 0.0 0.0 94-95 0.5 0.0 0.0 0.0 0.0 96-97 0.6625 0.0 0.0 0.0 0.0 98-99 0.9 0.0 0.0 0.0 0.0 100-101 1.125 0.0 0.0 0.0 0.0 102-103 1.5375 0.0 0.0 0.0 0.0 104-105 1.6749999999999998 0.0 0.0 0.0 0.0 106-107 2.05 0.0 0.0 0.0 0.0 108-109 2.425 0.0 0.0 0.0 0.0 110-111 2.75 0.0 0.0 0.0 0.0 112-113 3.0875 0.0 0.0 0.0 0.0 114-115 3.6 0.0 0.0 0.0 0.0 116-117 4.125 0.0 0.0 0.0 0.0 118-119 4.5625 0.0 0.0 0.0 0.0 120-121 4.8875 0.0 0.0 0.0 0.0 122-123 5.1875 0.0 0.0 0.0 0.0 124-125 5.8125 0.0 0.0 0.0 0.0 126-127 6.4625 0.0 0.0 0.0 0.0 128-129 7.275 0.0 0.0 0.0 0.0 130-131 8.0125 0.0 0.0 0.0 0.0 132-133 8.5375 0.0 0.0 0.0 0.0 134-135 9.212499999999999 0.0 0.0 0.0 0.0 136-137 9.8625 0.0 0.0 0.0 0.0 138-139 10.5 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR13695469 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695469_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.94925 37.0 37.0 37.0 37.0 37.0 2 36.22 37.0 37.0 37.0 37.0 37.0 3 36.1845 37.0 37.0 37.0 37.0 37.0 4 36.233 37.0 37.0 37.0 37.0 37.0 5 36.401 37.0 37.0 37.0 37.0 37.0 6 36.2325 37.0 37.0 37.0 37.0 37.0 7 36.199 37.0 37.0 37.0 37.0 37.0 8 36.2465 37.0 37.0 37.0 37.0 37.0 9 36.2465 37.0 37.0 37.0 37.0 37.0 10-14 36.23440000000001 37.0 37.0 37.0 37.0 37.0 15-19 36.249399999999994 37.0 37.0 37.0 37.0 37.0 20-24 36.170899999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.130250000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.08705 37.0 37.0 37.0 37.0 37.0 35-39 36.08935 37.0 37.0 37.0 37.0 37.0 40-44 36.048950000000005 37.0 37.0 37.0 37.0 37.0 45-49 36.03885 37.0 37.0 37.0 37.0 37.0 50-54 35.99025 37.0 37.0 37.0 37.0 37.0 55-59 36.05825 37.0 37.0 37.0 37.0 37.0 60-64 35.96464999999999 37.0 37.0 37.0 37.0 37.0 65-69 35.8765 37.0 37.0 37.0 37.0 37.0 70-74 35.90175 37.0 37.0 37.0 37.0 37.0 75-79 35.88825 37.0 37.0 37.0 37.0 37.0 80-84 35.935950000000005 37.0 37.0 37.0 37.0 37.0 85-89 35.82355 37.0 37.0 37.0 37.0 37.0 90-94 35.77385 37.0 37.0 37.0 37.0 37.0 95-99 35.85415 37.0 37.0 37.0 37.0 37.0 100-104 35.830650000000006 37.0 37.0 37.0 37.0 37.0 105-109 35.78405 37.0 37.0 37.0 37.0 37.0 110-114 35.66525 37.0 37.0 37.0 37.0 37.0 115-119 35.66785 37.0 37.0 37.0 37.0 37.0 120-124 35.62245 37.0 37.0 37.0 37.0 37.0 125-129 35.665049999999994 37.0 37.0 37.0 37.0 37.0 130-134 35.54825 37.0 37.0 37.0 37.0 37.0 135-139 35.571450000000006 37.0 37.0 37.0 37.0 37.0 140-144 35.47005 37.0 37.0 37.0 37.0 37.0 145-149 35.30955 37.0 37.0 37.0 34.6 37.0 150-151 34.98625 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 3.0 14 3.0 15 6.0 16 2.0 17 2.0 18 1.0 19 3.0 20 1.0 21 3.0 22 3.0 23 3.0 24 9.0 25 6.0 26 4.0 27 14.0 28 10.0 29 18.0 30 19.0 31 27.0 32 59.0 33 85.0 34 194.0 35 605.0 36 2711.0 37 208.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.214554579673774 22.283563362609787 13.952321204516938 26.549560853199498 2 28.299999999999997 24.725 28.475 18.5 3 20.575 28.075 31.075000000000003 20.275000000000002 4 23.625 32.675 23.375 20.325 5 27.275 36.525 20.3 15.9 6 20.474999999999998 39.725 21.85 17.95 7 21.325 22.650000000000002 36.3 19.725 8 22.625 25.4 26.275 25.7 9 22.650000000000002 25.775 30.349999999999998 21.224999999999998 10-14 23.72 28.794999999999998 25.759999999999998 21.725 15-19 24.36 28.465 26.41 20.765 20-24 22.8245649129826 29.610922184436888 25.980196039207843 21.584316863372674 25-29 23.970992748187047 28.042010502625658 26.156539134783696 21.8304576144036 30-34 22.77069267316829 28.6271567891973 27.806951737934483 20.795198799699925 35-39 23.845961490372595 28.212053013253314 27.00675168792198 20.935233808452114 40-44 24.47111777944486 27.291822955738937 27.291822955738937 20.94523630907727 45-49 22.35058764691173 28.312078019504877 27.70692673168292 21.630407601900476 50-54 23.410852713178297 28.392098024506122 27.28182045511378 20.9152288072018 55-59 23.545886471617905 27.49687421855464 27.33183295823956 21.6254063515879 60-64 24.056014003500874 27.08677169292323 27.121780445111277 21.735433858464617 65-69 23.10731073107311 28.57285728572857 26.63766376637664 21.682168216821683 70-74 23.91097774443611 28.37209302325581 27.136784196049014 20.580145036259065 75-79 23.6609152288072 27.71692923230808 27.291822955738937 21.330332583145786 80-84 23.07576894223556 26.846711677919483 27.866966741685424 22.210552638159538 85-89 23.315828957239308 28.35708927231808 27.016754188547136 21.310327581895475 90-94 24.111027756939237 26.926731682920728 27.956989247311824 21.005251312828207 95-99 24.17604401100275 28.02700675168792 27.22180545136284 20.575143785946487 100-104 23.365841460365093 29.037259314828706 26.776694173543387 20.820205051262818 105-109 22.90072518129532 29.18729682420605 26.921730432608154 20.990247561890474 110-114 24.50612653163291 27.41685421355339 27.51187796949237 20.56514128532133 115-119 24.66616654163541 28.387096774193548 26.376594148537137 20.57014253563391 120-124 24.621155288822205 28.172043010752688 26.70667666916729 20.500125031257816 125-129 24.641160290072516 28.047011752938232 27.021755438859714 20.29007251812953 130-134 25.041260315078766 28.482120530132534 26.556639159789945 19.919979994998748 135-139 25.01625406351588 28.042010502625658 27.151787946986744 19.789947486871718 140-144 25.566391597899475 27.326831707926978 26.591647911977994 20.51512878219555 145-149 26.30657664416104 27.80195048762191 26.47161790447612 19.419854963740935 150-151 26.544136034008503 28.582145536384097 25.23130782695674 19.642410602650664 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.5 6 0.5 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.5 13 0.5 14 1.0 15 2.0 16 1.5 17 0.5 18 0.5 19 0.5 20 1.5 21 1.5 22 4.5 23 5.5 24 1.0 25 0.5 26 5.5 27 6.0 28 6.0 29 6.0 30 11.5 31 16.0 32 18.5 33 29.5 34 42.0 35 61.0 36 70.0 37 88.0 38 107.5 39 137.0 40 190.0 41 217.5 42 241.0 43 261.5 44 256.0 45 266.0 46 262.5 47 241.0 48 226.0 49 200.0 50 181.0 51 177.0 52 143.0 53 107.0 54 91.0 55 70.5 56 60.5 57 46.0 58 41.0 59 33.0 60 20.0 61 14.5 62 7.5 63 4.5 64 2.0 65 0.0 66 0.0 67 0.0 68 1.5 69 1.5 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 1.0 78 1.0 79 0.0 80 0.0 81 0.5 82 0.5 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.375 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.02 25-29 0.025 30-34 0.025 35-39 0.025 40-44 0.025 45-49 0.025 50-54 0.025 55-59 0.025 60-64 0.025 65-69 0.01 70-74 0.025 75-79 0.025 80-84 0.025 85-89 0.025 90-94 0.025 95-99 0.025 100-104 0.025 105-109 0.025 110-114 0.025 115-119 0.025 120-124 0.025 125-129 0.025 130-134 0.025 135-139 0.025 140-144 0.025 145-149 0.025 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.9 #Duplication Level Percentage of deduplicated Percentage of total 1 68.55083459787556 45.175 2 19.233687405159333 25.35 3 7.283763277693475 14.399999999999999 4 3.1866464339908953 8.4 5 0.9104704097116844 3.0 6 0.5311077389984825 2.1 7 0.15174506828528073 0.7000000000000001 8 0.07587253414264036 0.4 9 0.03793626707132018 0.22499999999999998 >10 0.03793626707132018 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGATAATTAACTTCTGTTTACGGGAATTACGTCCATTACCGCAATAAACT 10 0.25 No Hit GTCAGGATTATGTAAAAAAACAAACTCGTTTTGTATCCCGCAAACCAGCA 9 0.22499999999999998 No Hit GGAAAACCACCAAGCCTGTTCCCTCCGGGAGCCCATGGTACGGACCAGAC 8 0.2 No Hit TGACAATTTGCTTCCTTGGTTGGCTCATCTTGCTGCTTCATGACACATTC 8 0.2 No Hit ACCACATATAGAGGGTGTAATAGCTAAGTAGCCTGTAAGAGATGGCTTCC 7 0.17500000000000002 No Hit GGATGGCCAATGGATCGATGTTCCGCCCATGAAACACTCCATTGTTATCA 7 0.17500000000000002 No Hit AGAAGATTTATATATATAATATTAATAAATAGTGGAGGATCACAACAGAT 7 0.17500000000000002 No Hit AGTTGATCAACTTGACAAGATTTTCCGAATCCTTGGTACACCTAATGAGA 7 0.17500000000000002 No Hit TTACCAACACTCGATCTCAAATCCAAGCCTATGTTTTTGATGTGATCAGG 6 0.15 No Hit CGTGATCTTAGATTGTTATCACCAAAATCGGAAGAGGATAAATCTTTAGT 6 0.15 No Hit GCAGAGAATGTTCAAAAGAGCAGGATTTTCTTCATTGCATGGTCCCCTGA 6 0.15 No Hit GGAGATTAGTAGGATATCTATTCCCACAGATTATGAGACTGGCGCTATTA 6 0.15 No Hit ATTTGCTAGCTAGAAGTGACTCTACCCTTTGCATTACTTTTTCAATCAAT 6 0.15 No Hit GTGAGGCTCTCTCATTGGTTTATACTTCAATATAAGCCTTGGTAGGGATA 6 0.15 No Hit GCCAGCGAAGCACTTTCAGCAATGGATGGCCAGAATTTGGGAGGAAGAAC 6 0.15 No Hit CAGTGGGAATCCTTATGGTATAGCACAGGATATAGTTTTCAGCATGCCTT 6 0.15 No Hit CAGAGAACACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 6 0.15 No Hit TTCGGATCTCTTGGCTTGATGACCTCTGTATTGGTGTGCCCAGATGGAAA 6 0.15 No Hit CATCGATCAGGCAGCAGTTCTTCCAGCTCTTCTAGCGACGAGGAGGAAGG 6 0.15 No Hit TAAGATGCGTGAGGAGTACCCTCATCTTTTCGATAATCTCGATAATCCTG 6 0.15 No Hit CCCTAACTTAATGGACGGGAGGTATCCCAATAGGAGGTTTCCTCCTATGG 6 0.15 No Hit GGCCATCTGGGCTACACAGGTGGTCTTGATGGGTGCCGTTGAGGGTTACA 6 0.15 No Hit GGATAGTAGTAGTAGAGACTAGAGAGGTACAAGCATACCATGGCTAGGTT 5 0.125 No Hit AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 5 0.125 No Hit GCAGTGCCTCAGTCCAGCCTCCCAGACTCAGAGAGAGAGAGAGAGAGAGA 5 0.125 No Hit GGAAAGCTTTATGGACTATCACTTTGCATAAGATATTTGCAGCAATCGCA 5 0.125 No Hit GAGTCAGAGTACATGCAGGCTTGGATTGTTTCTTGTGGACTGAGCAAATG 5 0.125 No Hit GAGCTTCATTGAGAGCCAAGTAAAGAAAATCTCGGAGTTGAGAAGAATGT 5 0.125 No Hit CCCTGATTTAAGGATCATGCAATCATTAGGAGCCAAAGTTCGTTCATTTC 5 0.125 No Hit GCAGAAGCTAAGTCAGCAATGGCAGCCTCAGTAATGGCTTCATTGAGCCT 5 0.125 No Hit CTCACAAACGCAGCTTCACACTTGTTCACGGGTTCTAAGAGAATGGCCAC 5 0.125 No Hit GTATAATCTGAATTTGAAGTTGTAAATGTTATATTTTGTTTAGTTATCTT 5 0.125 No Hit GTGTTGGAGTTCCACCAGTACACTCCTGGCTCACGGTGGAGAAGCACTAC 5 0.125 No Hit ATGGGTTTGGCAGGATCGGCAGAAACTTCTTGAGGTGCTGGCATGGACGC 5 0.125 No Hit GGTTCTGCTGCGGTACATCCCTTTAACTGCAAAGCGCATGACAGGAATTG 5 0.125 No Hit CCTGATGTTGTGTCCATGAATCAGAAAGAAATGTCAGCCACTGAGTTCAT 5 0.125 No Hit ATCCGTGGAAGCACAGCAGCAAAGTGATCAAAGTGATTGCATTAATGCAG 5 0.125 No Hit GGTATGGGTCGGAGCCGTAGCAGGAGTAGGAGTCCCCGAGATCGCAGATT 5 0.125 No Hit ATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAATA 5 0.125 No Hit TCTCAGCAAGAAGCCAGACGACTTCGAAAAATATCAGGCATTTGAGTTGA 5 0.125 No Hit GCAAGTTCTGATTCTAATGCTTTTCCTGCGACTGTAATATTGGAGGGCGT 5 0.125 No Hit ATTACCAAAACCTCCAAGGAGCCGGTCTTTAGAATTCAAGCTGCAAAACT 5 0.125 No Hit AAAGCTGTCATTGAAGCAAAGGAGTATGTGGAGAAAGAAACTGCTGAAAA 5 0.125 No Hit AGAAGACACAAAACTGAAAGAAAAGGCAGCAATAACCAATCTTCATTCAG 5 0.125 No Hit GTATCATTGTGGAATTTGCAAAGGGGGTACCTCGTGGTTCTCGAGAATAT 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0 0.0 0.0 0.0 0.0 84-85 0.025 0.0 0.0 0.0 0.0 86-87 0.05 0.0 0.0 0.0 0.0 88-89 0.1 0.0 0.0 0.0 0.0 90-91 0.16249999999999998 0.0 0.0 0.0 0.0 92-93 0.3125 0.0 0.0 0.0 0.0 94-95 0.475 0.0 0.0 0.0 0.0 96-97 0.6375 0.0 0.0 0.0 0.0 98-99 0.875 0.0 0.0 0.0 0.0 100-101 1.1 0.0 0.0 0.0 0.0 102-103 1.5125 0.0 0.0 0.0 0.0 104-105 1.65 0.0 0.0 0.0 0.0 106-107 2.025 0.0 0.0 0.0 0.0 108-109 2.4 0.0 0.0 0.0 0.0 110-111 2.7249999999999996 0.0 0.0 0.0 0.0 112-113 3.0625 0.0 0.0 0.0 0.0 114-115 3.575 0.0 0.0 0.0 0.0 116-117 4.125 0.0 0.0 0.0 0.0 118-119 4.5625 0.0 0.0 0.0 0.0 120-121 4.8875 0.0 0.0 0.0 0.0 122-123 5.1875 0.0 0.0 0.0 0.0 124-125 5.8375 0.0 0.0 0.0 0.0 126-127 6.512499999999999 0.0 0.0 0.0 0.0 128-129 7.325 0.0 0.0 0.0 0.0 130-131 8.0625 0.0 0.0 0.0 0.0 132-133 8.5875 0.0 0.0 0.0 0.0 134-135 9.2625 0.0 0.0 0.0 0.0 136-137 9.925 0.0 0.0 0.0 0.0 138-139 10.575 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTGGTGC 10 0.006830828 145.0 1 >>END_MODULE Read 676050 spots for SRR13695469.sra Written 676050 spots for SRR13695469.sra Read 676050 spots for SRR13695469.sra Written 676050 spots for SRR13695469.sra Read 676050 spots for SRR13695469.sra Written 676050 spots for SRR13695469.sra Read 676050 spots for SRR13695469.sra Written 676050 spots for SRR13695469.sra Read 676050 spots for SRR13695469.sra Written 676050 spots for SRR13695469.sra Read 676050 spots for SRR13695469.sra Written 676050 spots for SRR13695469.sra Read 676050 spots for SRR13695469.sra Written 676050 spots for SRR13695469.sra Read 676050 spots for SRR13695469.sra Written 676050 spots for SRR13695469.sra Read 676050 spots for SRR13695469.sra Written 676050 spots for SRR13695469.sra Read 676050 spots for SRR13695469.sra Written 676050 spots for SRR13695469.sra Read 676050 spots for SRR13695469.sra Written 676050 spots for SRR13695469.sra Read 676050 spots for SRR13695469.sra Written 676050 spots for SRR13695469.sra Read 676050 spots for SRR13695469.sra Written 676050 spots for SRR13695469.sra Read 676050 spots for SRR13695469.sra Written 676050 spots for SRR13695469.sra Read 676050 spots for SRR13695469.sra Written 676050 spots for SRR13695469.sra Read 676050 spots for SRR13695469.sra Written 676050 spots for SRR13695469.sra Read 676065 spots for SRR13695469.sra Written 676065 spots for SRR13695469.sra Read 676050 spots for SRR13695469.sra Written 676050 spots for SRR13695469.sra Read 676050 spots for SRR13695469.sra Written 676050 spots for SRR13695469.sra Read 676050 spots for SRR13695469.sra Written 676050 spots for SRR13695469.sra SRR ids: ['SRR13695469.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_7hlndj0z SRR13695469.sra spots: 13521015 blocks: [[1, 676050], [676051, 1352100], [1352101, 2028150], [2028151, 2704200], [2704201, 3380250], [3380251, 4056300], [4056301, 4732350], [4732351, 5408400], [5408401, 6084450], [6084451, 6760500], [6760501, 7436550], [7436551, 8112600], [8112601, 8788650], [8788651, 9464700], [9464701, 10140750], [10140751, 10816800], [10816801, 11492850], [11492851, 12168900], [12168901, 12844950], [12844951, 13521015]] SRR13695469 file size 4573332 SRR13695469 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695469 SRR13695469_1.fastq SRR13695469_2.fastq Input file: SRR13695469_1.fastq Paired file: SRR13695469_2.fastq trimmed: SRR13695469-trimmed-pair1.fastq, SRR13695469-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 05:16:31 2025 >> started Wed Feb 12 05:16:47 2025 >> done (15.721s) 13521015 read pairs processed; of these: 112 ( 0.00%) short read pairs filtered out after trimming by size control 4040 ( 0.03%) empty read pairs filtered out after trimming by size control 13516863 (99.97%) read pairs available; of these: 1997266 (14.78%) trimmed read pairs available after processing 11519597 (85.22%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 21 1 0.00% 22 0 0.00% 23 1 0.00% 24 0 0.00% 25 2 0.00% 26 1 0.00% 27 4 0.00% 28 2 0.00% 29 4 0.00% 30 1 0.00% 31 1 0.00% 32 2 0.00% 33 4 0.00% 34 5 0.00% 35 3 0.00% 36 5 0.00% 37 4 0.00% 38 4 0.00% 39 10 0.00% 40 23 0.00% 41 10 0.00% 42 15 0.00% 43 9 0.00% 44 13 0.00% 45 29 0.00% 46 27 0.00% 47 36 0.00% 48 26 0.00% 49 30 0.00% 50 64 0.00% 51 80 0.00% 52 66 0.00% 53 75 0.00% 54 55 0.00% 55 96 0.00% 56 99 0.00% 57 113 0.00% 58 131 0.00% 59 132 0.00% 60 170 0.00% 61 256 0.00% 62 243 0.00% 63 326 0.00% 64 314 0.00% 65 419 0.00% 66 399 0.00% 67 424 0.00% 68 597 0.00% 69 615 0.00% 70 736 0.01% 71 848 0.01% 72 938 0.01% 73 1165 0.01% 74 1251 0.01% 75 1449 0.01% 76 1724 0.01% 77 1920 0.01% 78 2081 0.02% 79 2286 0.02% 80 2699 0.02% 81 2894 0.02% 82 3348 0.02% 83 3677 0.03% 84 4327 0.03% 85 4755 0.04% 86 5130 0.04% 87 5503 0.04% 88 5987 0.04% 89 6471 0.05% 90 7217 0.05% 91 7632 0.06% 92 8433 0.06% 93 9294 0.07% 94 9816 0.07% 95 10831 0.08% 96 11612 0.09% 97 12476 0.09% 98 13091 0.10% 99 13779 0.10% 100 14368 0.11% 101 14846 0.11% 102 15673 0.12% 103 16573 0.12% 104 17461 0.13% 105 18500 0.14% 106 19665 0.15% 107 20724 0.15% 108 21245 0.16% 109 22506 0.17% 110 22490 0.17% 111 23465 0.17% 112 24482 0.18% 113 25469 0.19% 114 26119 0.19% 115 27421 0.20% 116 28240 0.21% 117 29956 0.22% 118 30878 0.23% 119 30909 0.23% 120 31971 0.24% 121 32907 0.24% 122 33187 0.25% 123 34600 0.26% 124 35419 0.26% 125 36255 0.27% 126 37451 0.28% 127 38722 0.29% 128 39117 0.29% 129 39722 0.29% 130 41243 0.31% 131 41718 0.31% 132 41830 0.31% 133 43152 0.32% 134 43233 0.32% 135 44566 0.33% 136 45532 0.34% 137 45930 0.34% 138 47160 0.35% 139 49000 0.36% 140 48884 0.36% 141 49032 0.36% 142 50954 0.38% 143 50411 0.37% 144 51580 0.38% 145 52508 0.39% 146 52424 0.39% 147 53294 0.39% 148 54212 0.40% 149 55698 0.41% 150 56248 0.42% 151 11519597 85.22% 13516863 reads passed initial QC criterion=sequence-density sequence-density=0.80 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=22 prefix-density=0.78 prefix-fanout=2.0 sequence=GTGTTGTCGAATCC criterion=fanout-score sequence-density=0.02 sequence-density-rank=25 fanout-score=36.24 fanout-score-rank=1 prefix-density=0.09 prefix-fanout=7.4 sequence=AAACAGAATATTTACTTTTAGCGCAAGTTTCAATTCATGAGCTCGTTACATCACAAGTTAACATTACAAGCCAATAGTCCCAGCACAGAAAAAACTCTTTTGTTGCTCACTTTCCGGGAACGAAGTTTGTGGCATATGCCCAGGCGTTGTTGTTTACTGGGTCAGAAAGGTGGTCAGCCAGGTTCTCCAGTGGTCCCTTTCCGGTCACAATGGCCTGGACAAAGAATCCGAACATTGAGAACATAGCCAA criterion=sequence-density sequence-density=0.59 sequence-density-rank=1 fanout-score=1.96 fanout-score-rank=29 prefix-density=0.58 prefix-fanout=2.0 sequence=TACCTTCTTCGC criterion=fanout-score sequence-density=0.03 sequence-density-rank=26 fanout-score=21.32 fanout-score-rank=1 prefix-density=0.50 prefix-fanout=1.4 sequence=TTACTTCCATTTCCGCCCAAGCTGCTCACAGTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGGCTAGACTAGCGTACGAGCACTATGGTCAGTAATTCCTGGAGGAATAGGTACCAAGAAAAAAACGAACCTTTGGGTTCCAGAGCTGTACGGTCGCACTGAACTCGGATAGGTCTCAGAAAAACGAAATATAGGCTTACGGTAGGTCCGAATGGCACAAAGCTTGTTCCGTTAGCTGGCATAAGATTCCATGCCTAGATGTGATACACGTTTCTGGAAACTGCCTCGTCATGCGACTGTTCCCCGGGGTCAGGGCCGCTGGTATTTGCTGTAAAGAGGGGCGTTGAGTCCGTCCGACTTCACTGCCCCCTTTCAGCCTTTTGGGTCCTGTATCCCAATTCTCAGAGGTCCCGCCGTACGCTGAGGACCACCTGAAACGGGCATCGTCGCTCTTCGTTGTTCGTCGACTTCTAGTGTGGAGACGAATTGCCAGA SRR13695469 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 05:17:31 Started mapping on | Feb 12 05:17:32 Finished on | Feb 12 05:19:56 Mapping speed, Million of reads per hour | 337.92 Number of input reads | 13516863 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 12173312 Uniquely mapped reads % | 90.06% Average mapped length | 293.52 Number of splices: Total | 11604053 Number of splices: Annotated (sjdb) | 11396379 Number of splices: GT/AG | 11357209 Number of splices: GC/AG | 211940 Number of splices: AT/AC | 5879 Number of splices: Non-canonical | 29025 Mismatch rate per base, % | 0.37% Deletion rate per base | 0.03% Deletion average length | 2.97 Insertion rate per base | 0.02% Insertion average length | 2.31 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 287897 % of reads mapped to multiple loci | 2.13% Number of reads mapped to too many loci | 92344 % of reads mapped to too many loci | 0.68% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 6.96% % of reads unmapped: other | 0.17% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1055823 1055823 1055823 N_multimapping 287897 287897 287897 N_noFeature 316846 11913329 423694 N_ambiguous 229328 1060 75699 UnstrandedReadsAssigned:11627138 PositiveStrandReadsAssigned:258923 NegativeStrandReadsAssigned:11673919 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695469 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695469-trimmed-pair1.fastq SRR13695469-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 13,516,863 reads, 11,802,994 reads pseudoaligned [quant] estimated average fragment length: 229.39 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,235 rounds 52401 SRR13695469.ke.tsv 34699 SRR13695469.se.tsv 87100 total ==> SRR13695469.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1789.61 245 9.34704 Potri.005G024800.1.v4.1 1035 806.61 224 18.9605 Potri.004G059700.1.v4.1 961 732.626 10 0.931932 Potri.007G009000.2.v4.1 1416 1187.61 0 0 Potri.003G141000.2.v4.1 2943 2714.61 522.501 13.1415 Potri.016G087400.1.v4.1 270 87.8019 439.813 342.003 Potri.015G069301.1.v4.1 564 339.58 0 0 Potri.010G195200.1.v4.1 1773 1544.61 91 4.02243 Potri.012G127500.1.v4.1 977 748.621 131 11.9475 ==> SRR13695469.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 156 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 320 Potri.001G212900.v4.1 33 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 0 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 4 SRR13695469 completed mapping pipeline successfully