Starting /dee2/code/volunteer_pipeline.sh SRR13695470 current disk space = 3049665671168 free memory = 1515674892 SRR13695470 SRAfilesize 7694d01ca58d4b4a833e1c0432626336 SRR13695470.sra SRR13695470.sra file validated SRR13695470 is paired end SRR13695470 is conventional basespace SRR13695470 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695470_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.508 37.0 37.0 37.0 37.0 37.0 2 36.232 37.0 37.0 37.0 37.0 37.0 3 36.523 37.0 37.0 37.0 37.0 37.0 4 36.492 37.0 37.0 37.0 37.0 37.0 5 36.5005 37.0 37.0 37.0 37.0 37.0 6 36.476 37.0 37.0 37.0 37.0 37.0 7 36.432 37.0 37.0 37.0 37.0 37.0 8 36.5415 37.0 37.0 37.0 37.0 37.0 9 36.547 37.0 37.0 37.0 37.0 37.0 10-14 36.5368 37.0 37.0 37.0 37.0 37.0 15-19 36.5045 37.0 37.0 37.0 37.0 37.0 20-24 36.4707 37.0 37.0 37.0 37.0 37.0 25-29 36.403800000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.377300000000005 37.0 37.0 37.0 37.0 37.0 35-39 36.40560000000001 37.0 37.0 37.0 37.0 37.0 40-44 36.42 37.0 37.0 37.0 37.0 37.0 45-49 36.3394 37.0 37.0 37.0 37.0 37.0 50-54 36.321999999999996 37.0 37.0 37.0 37.0 37.0 55-59 36.3002 37.0 37.0 37.0 37.0 37.0 60-64 36.314 37.0 37.0 37.0 37.0 37.0 65-69 36.2709 37.0 37.0 37.0 37.0 37.0 70-74 36.28680000000001 37.0 37.0 37.0 37.0 37.0 75-79 36.2654 37.0 37.0 37.0 37.0 37.0 80-84 36.2257 37.0 37.0 37.0 37.0 37.0 85-89 36.1622 37.0 37.0 37.0 37.0 37.0 90-94 36.1272 37.0 37.0 37.0 37.0 37.0 95-99 36.0635 37.0 37.0 37.0 37.0 37.0 100-104 36.054700000000004 37.0 37.0 37.0 37.0 37.0 105-109 36.126200000000004 37.0 37.0 37.0 37.0 37.0 110-114 36.061899999999994 37.0 37.0 37.0 37.0 37.0 115-119 36.0376 37.0 37.0 37.0 37.0 37.0 120-124 35.962 37.0 37.0 37.0 37.0 37.0 125-129 35.9277 37.0 37.0 37.0 37.0 37.0 130-134 35.924899999999994 37.0 37.0 37.0 37.0 37.0 135-139 35.9243 37.0 37.0 37.0 37.0 37.0 140-144 35.8101 37.0 37.0 37.0 37.0 37.0 145-149 35.7141 37.0 37.0 37.0 37.0 37.0 150-151 35.3875 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 1.0 23 1.0 24 1.0 25 4.0 26 8.0 27 11.0 28 14.0 29 17.0 30 30.0 31 45.0 32 47.0 33 82.0 34 128.0 35 312.0 36 2932.0 37 367.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.625 12.975 6.825 40.575 2 18.897835933568192 14.091595369904377 38.50025163563161 28.510317060895822 3 17.849999999999998 18.099999999999998 27.650000000000002 36.4 4 24.175 23.375 23.849999999999998 28.599999999999998 5 22.875 32.35 24.349999999999998 20.424999999999997 6 19.900000000000002 33.25 24.425 22.425 7 15.825 27.650000000000002 40.050000000000004 16.475 8 18.224999999999998 26.55 31.35 23.875 9 17.95 22.725 34.875 24.45 10-14 19.225 29.099999999999998 27.860000000000003 23.815 15-19 19.134999999999998 28.605000000000004 28.349999999999998 23.91 20-24 20.28 27.665 28.405 23.65 25-29 19.725 28.7 28.275 23.3 30-34 19.125 29.025000000000002 27.43 24.42 35-39 20.26 28.4 27.62 23.72 40-44 19.46 29.2 27.465 23.875 45-49 19.885 28.645 27.375 24.095 50-54 20.765 28.485 27.500000000000004 23.25 55-59 20.419999999999998 29.14 26.919999999999998 23.52 60-64 19.89 29.020000000000003 28.084999999999997 23.005 65-69 19.830000000000002 28.615000000000002 26.995 24.560000000000002 70-74 20.57 28.365000000000002 27.615000000000002 23.45 75-79 20.06 28.22 27.77 23.95 80-84 20.155 27.965 27.66 24.22 85-89 19.6 28.79 27.67 23.94 90-94 20.57 28.51 27.455000000000002 23.465 95-99 20.085 28.9 27.084999999999997 23.93 100-104 20.57 27.955000000000002 28.365000000000002 23.11 105-109 20.39 28.1 28.16 23.35 110-114 20.4 28.255000000000003 28.215 23.13 115-119 20.91 28.549999999999997 27.229999999999997 23.31 120-124 20.275000000000002 27.98 27.544999999999998 24.2 125-129 21.55 28.48 26.290000000000003 23.68 130-134 20.905 28.23 27.575 23.29 135-139 21.05 28.610000000000003 26.61 23.73 140-144 20.5 28.21 26.96 24.33 145-149 20.735 28.51 26.924999999999997 23.830000000000002 150-151 19.9125 28.849999999999998 27.200000000000003 24.0375 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 0.5 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.5 12 0.5 13 0.0 14 0.0 15 0.0 16 0.5 17 0.5 18 0.0 19 0.0 20 0.0 21 1.0 22 1.0 23 2.0 24 5.0 25 4.5 26 5.0 27 7.5 28 9.5 29 15.0 30 25.5 31 33.0 32 37.0 33 45.0 34 53.5 35 69.5 36 109.0 37 123.0 38 118.5 39 142.0 40 173.5 41 203.5 42 241.5 43 254.5 44 252.5 45 260.5 46 244.5 47 254.5 48 256.5 49 199.0 50 154.5 51 146.5 52 128.0 53 98.0 54 77.0 55 51.0 56 35.5 57 38.0 58 35.5 59 23.5 60 18.5 61 14.5 62 8.5 63 6.5 64 3.0 65 0.5 66 0.0 67 1.5 68 1.5 69 0.0 70 0.0 71 0.0 72 0.0 73 3.0 74 3.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.65 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.27499999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 68.65333836288194 45.5 2 18.93625047152018 25.1 3 8.03470388532629 15.975 4 2.7536778574122973 7.3 5 1.018483591097699 3.375 6 0.4149377593360996 1.6500000000000001 7 0.03772161448509996 0.17500000000000002 8 0.03772161448509996 0.2 9 0.03772161448509996 0.22499999999999998 >10 0.07544322897019992 0.5 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGC 10 0.25 No Hit CTTTTTGGCATATAGGATCTTATCATAAACTGAGGGCGAATACCCCATTG 10 0.25 No Hit GAAGGTAATGCTCCAAAACAGCCAAGAGCTCTTTCCCTTGGCTCACATCT 9 0.22499999999999998 No Hit AGAAGAGATGTCACTGTAGTCAACGGTAGGAATAAGCTGTCGAAGTTCGT 8 0.2 No Hit GCGGGCAAGACCCCAGGCAGCCTCCTTCACTGCCAAGGCAGATGGGCCGT 7 0.17500000000000002 No Hit GTACGTGTCCCAATAATATCTCCAGCCAGATTCTTAGCCAAGTTTTGGTC 6 0.15 No Hit CGTTAATCTTGCCAGGAACGATGCTCCCTGTGGTGTTGTAAGCTATGTAC 6 0.15 No Hit CCTCAACTTTCGTTCTTGATTAATGAAAACATCCTTGGCAAATGCTTTCG 6 0.15 No Hit CACCACATCACCATCTCTGAAGAATTTAACTTTAACTGCAAATCAATTCA 6 0.15 No Hit GGGGCTTTGGCCTTTGGCAAGCTCGGAGTACAGTCCCGGATTCTTGTCAT 6 0.15 No Hit CAACAACAAGCAAAAATGCTAATGCTCACAAATTATACAAGGTAGAAAAC 6 0.15 No Hit CTTCTCAGCAGCAGCAAGGCTGTCGCTAATCCTTTCTGCGGTCTCGAAAT 6 0.15 No Hit ACTCCCTGTATTTCTCTGGTGTGTGATCTGGAGATTTTCCAGAGAAAAAA 6 0.15 No Hit GTCTCCACACTAGAAGTCGACGAACAACGAAGAGCGACGATGCCCGTTTC 6 0.15 No Hit GTCTTGTCAATGTTGTAATGACTGGTCTATGGTGAGCAGGAACATTTGAG 6 0.15 No Hit CCCGACTCGCAGACAGCGCCTCGTGGTGCGGCAGGGTCCAGCCACGACGG 6 0.15 No Hit GTTTCCGATGCAGCAACAGAATCAAGGAGTGCTGGTGTATCTTCTTCAGG 5 0.125 No Hit CTCCACTTTTCTCTTACACCCTTTTCTACTATCATTCACATCCACAGCCT 5 0.125 No Hit TTGGCCTCTTTCCTTTAACGGGATTCTCCTCCACCTTAGGCTCCTCCGTA 5 0.125 No Hit CATGTCGACAAATTCGTTTTCCTTCATTTTCTTGAAGAAAACTTGAGAAA 5 0.125 No Hit GTGAACATCATTACTATCCAATATTGAAACCGACTGGATTGTAGTCGACG 5 0.125 No Hit GTCAATGGTTGAAGAAACATCAGAGCATCGGAAGTCAACAGACACAAGAG 5 0.125 No Hit ATTGCCTTTAGCTTTGAGTTTCTCAGCTGCCTCATCTACATTTTTCTGCT 5 0.125 No Hit GCTGAAACGAACTCTTTCCACTTTCCCTTTCTTGACCGCATTCAGAAACT 5 0.125 No Hit GGGGATGATACTCATAAACACAAGAAGAAAAAAAATCTCTAAAAAGATCC 5 0.125 No Hit TCTGCATATTCCCATTTGCATAAAAGTCTGTCATAGAAACCAATCCATTG 5 0.125 No Hit GGCAAATGGGACAAAAGGAGGCAGCACACCAGTCAGCGATGCCATTCCTC 5 0.125 No Hit TTTTTTTTTTATGCAAAATCGATTCTCATAATTCAACATCATTGCCCTTT 5 0.125 No Hit CAACAAATCTCATCCATGTCCAACAACAAAAAACAAACAAAAGTTGATAA 5 0.125 No Hit GGGGTGGTGTCAACATCAGTAATGTTGATGAAATTGGAAAAAGCAGTCTG 5 0.125 No Hit CACGGTGATATCAGAAGAGATGATGGAGTGAATGGGTTTGTTAGGGGAAG 5 0.125 No Hit ATGCAGAAGCAGTTTTCACACCCTCGAGTATACCAGTTCTTGTTATAAAC 5 0.125 No Hit GCGCTCTGCTTCAGCCAGAAGGGCCATCCTCCTAGCAGTCTTAGCATATG 5 0.125 No Hit GCGAATCATATTAAACTTTTTTGAACTCGTCCTAAACTAAAGCTATGAAA 5 0.125 No Hit TGATCCTGAACGGCTCAGGGCCCATAGATAGAGCAGCGACAACACAGAGA 5 0.125 No Hit CCTCCATCAAACTTTGAGAACTTCATAGAATCAAGTCTGATAGATTTAGG 5 0.125 No Hit AGCAAGACAACACATAAATTGCACAGATAAATTATGAAAGTTTTGGTACA 5 0.125 No Hit CTGATGTAGAAAAACTCCATGTCTATGAAACCAACATCTCCTTGTTCATC 5 0.125 No Hit GTTGGTGTGTAGCCCCCATGGTGTTTTGGGCTTCTGTTGGGTGTCCCTGG 5 0.125 No Hit CATGGGAGGTTGGGATCATCAACAAAAGAGGTTACTTGCCTCTCCAATTT 5 0.125 No Hit TCTAAGAATTAGCAGGTGAGCACTAACAGAGGGTATCAACAAAAAGAAGT 5 0.125 No Hit CCATTTTTAGTTATAATGATGCCTTATGTGATAGATGCCTCTTTAAAATA 5 0.125 No Hit GTGAATGATAGCTCGAGTTCAGTGATATCATCTTGAGCCTCAAGAGTAGC 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.05 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.05 0.0 0.0 0.0 0.0 72-73 0.0875 0.0 0.0 0.0 0.0 74-75 0.1 0.0 0.0 0.0 0.0 76-77 0.1 0.0 0.0 0.0 0.0 78-79 0.1 0.0 0.0 0.0 0.0 80-81 0.1 0.0 0.0 0.0 0.0 82-83 0.1 0.0 0.0 0.0 0.0 84-85 0.1 0.0 0.0 0.0 0.0 86-87 0.125 0.0 0.0 0.0 0.0 88-89 0.15 0.0 0.0 0.0 0.0 90-91 0.175 0.0 0.0 0.0 0.0 92-93 0.225 0.0 0.0 0.0 0.0 94-95 0.25 0.0 0.0 0.0 0.0 96-97 0.2875 0.0 0.0 0.0 0.0 98-99 0.4 0.0 0.0 0.0 0.0 100-101 0.5375 0.0 0.0 0.0 0.0 102-103 0.7625 0.0 0.0 0.0 0.0 104-105 0.8875 0.0 0.0 0.0 0.0 106-107 0.9375 0.0 0.0 0.0 0.0 108-109 1.025 0.0 0.0 0.0 0.0 110-111 1.3250000000000002 0.0 0.0 0.0 0.0 112-113 1.7 0.0 0.0 0.0 0.0 114-115 1.9874999999999998 0.0125 0.0 0.0 0.0 116-117 2.175 0.025 0.0 0.0 0.0 118-119 2.4000000000000004 0.025 0.0 0.0 0.0 120-121 2.625 0.025 0.0 0.0 0.0 122-123 2.875 0.025 0.0 0.0 0.0 124-125 3.15 0.025 0.0 0.0 0.0 126-127 3.5875000000000004 0.025 0.0 0.0 0.0 128-129 4.1875 0.025 0.0 0.0 0.0 130-131 4.5375 0.025 0.0 0.0 0.0 132-133 5.1 0.025 0.0 0.0 0.0 134-135 5.6 0.025 0.0 0.0 0.0 136-137 5.975 0.025 0.0 0.0 0.0 138-139 6.325 0.025 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATCTAC 10 0.006830828 145.0 5 AATGTAC 10 0.006830828 145.0 9 >>END_MODULE SRR13695470 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695470_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 35.9985 37.0 37.0 37.0 37.0 37.0 2 35.895 37.0 37.0 37.0 37.0 37.0 3 35.9055 37.0 37.0 37.0 37.0 37.0 4 35.8355 37.0 37.0 37.0 37.0 37.0 5 36.006 37.0 37.0 37.0 37.0 37.0 6 35.9475 37.0 37.0 37.0 37.0 37.0 7 36.083 37.0 37.0 37.0 37.0 37.0 8 36.1015 37.0 37.0 37.0 37.0 37.0 9 36.0475 37.0 37.0 37.0 37.0 37.0 10-14 36.037600000000005 37.0 37.0 37.0 37.0 37.0 15-19 35.9293 37.0 37.0 37.0 37.0 37.0 20-24 35.930600000000005 37.0 37.0 37.0 37.0 37.0 25-29 35.8744 37.0 37.0 37.0 37.0 37.0 30-34 35.83069999999999 37.0 37.0 37.0 37.0 37.0 35-39 35.812200000000004 37.0 37.0 37.0 37.0 37.0 40-44 35.84439999999999 37.0 37.0 37.0 37.0 37.0 45-49 35.8002 37.0 37.0 37.0 37.0 37.0 50-54 35.6845 37.0 37.0 37.0 37.0 37.0 55-59 35.673500000000004 37.0 37.0 37.0 37.0 37.0 60-64 35.6365 37.0 37.0 37.0 37.0 37.0 65-69 35.642649999999996 37.0 37.0 37.0 37.0 37.0 70-74 35.637800000000006 37.0 37.0 37.0 37.0 37.0 75-79 35.60505 37.0 37.0 37.0 37.0 37.0 80-84 35.6278 37.0 37.0 37.0 37.0 37.0 85-89 35.4702 37.0 37.0 37.0 37.0 37.0 90-94 35.52115 37.0 37.0 37.0 37.0 37.0 95-99 35.4363 37.0 37.0 37.0 37.0 37.0 100-104 35.4364 37.0 37.0 37.0 37.0 37.0 105-109 35.4393 37.0 37.0 37.0 37.0 37.0 110-114 35.27569999999999 37.0 37.0 37.0 32.2 37.0 115-119 35.41270000000001 37.0 37.0 37.0 37.0 37.0 120-124 35.3457 37.0 37.0 37.0 37.0 37.0 125-129 35.4075 37.0 37.0 37.0 37.0 37.0 130-134 35.220299999999995 37.0 37.0 37.0 29.8 37.0 135-139 35.199 37.0 37.0 37.0 32.2 37.0 140-144 35.0655 37.0 37.0 37.0 25.0 37.0 145-149 35.0212 37.0 37.0 37.0 27.4 37.0 150-151 34.614000000000004 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 1.0 13 4.0 14 5.0 15 3.0 16 3.0 17 1.0 18 2.0 19 2.0 20 0.0 21 2.0 22 1.0 23 4.0 24 9.0 25 11.0 26 14.0 27 19.0 28 26.0 29 26.0 30 34.0 31 62.0 32 78.0 33 119.0 34 263.0 35 711.0 36 2472.0 37 126.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 38.75502008032129 22.31425702811245 10.266064257028113 28.664658634538153 2 28.599999999999998 26.174999999999997 31.05 14.174999999999999 3 21.375 27.150000000000002 30.049999999999997 21.425 4 26.275 33.0 22.45 18.275 5 24.474999999999998 36.5 21.85 17.175 6 20.4 38.4 23.474999999999998 17.724999999999998 7 22.5 22.725 36.675000000000004 18.099999999999998 8 21.224999999999998 25.3 29.175 24.3 9 20.349999999999998 25.900000000000002 31.175000000000004 22.575 10-14 23.54 29.020000000000003 27.08 20.36 15-19 22.79 28.9 27.055 21.255 20-24 22.07324394636782 28.952371422853712 27.86171703021813 21.112667600560336 25-29 22.162162162162165 28.893893893893896 28.063063063063066 20.88088088088088 30-34 22.87329863891113 27.837269815852682 27.987389911929544 21.302041633306644 35-39 22.708625175105063 28.61717030218131 27.996798078847306 20.67740644386632 40-44 23.038430744595676 27.917333867093674 27.90232185748599 21.14191353082466 45-49 22.700890623436408 28.6600620434304 27.909536675672967 20.729510657460224 50-54 22.84870922553532 28.357014208525115 28.14188513107865 20.652391434860917 55-59 22.84784784784785 28.07807807807808 28.058058058058062 21.016016016016014 60-64 23.514108465079048 27.501500900540325 27.88673203922353 21.097658595157096 65-69 23.33316660831291 27.52463362176762 27.529635372380334 21.61256439753914 70-74 23.173173173173172 27.63263263263263 28.263263263263262 20.93093093093093 75-79 22.459090226692688 27.97878196466997 27.893709653205224 21.668418155432118 80-84 23.08654327163582 27.458729364682345 28.50425212606303 20.95047523761881 85-89 23.18818818818819 27.792792792792792 27.757757757757755 21.26126126126126 90-94 23.610346725371492 28.033221594036124 27.387802071346375 20.96862960924601 95-99 23.23394036421853 28.572143285971585 27.891735041024614 20.302181308785272 100-104 22.907907907907905 28.44844844844845 27.547547547547545 21.096096096096094 105-109 23.158158158158155 27.81781781781782 28.393393393393396 20.63063063063063 110-114 22.898739243546128 28.6972183309986 27.911747048228936 20.492295377226334 115-119 23.823823823823822 28.233233233233236 28.008008008008005 19.934934934934933 120-124 23.913913913913916 28.143143143143146 27.467467467467465 20.475475475475474 125-129 23.836452807526772 27.97517765989391 27.780002001801623 20.4083675307777 130-134 23.643643643643646 28.86886886886887 27.43743743743744 20.05005005005005 135-139 24.96996996996997 27.47747747747748 27.54254254254254 20.01001001001001 140-144 24.279567740644385 28.47208324994997 27.076245747448468 20.172103261957176 145-149 25.25025025025025 28.78878878878879 27.087087087087085 18.873873873873872 150-151 25.988488488488485 27.852852852852855 26.664164164164166 19.494494494494493 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.5 3 0.5 4 0.5 5 0.5 6 0.5 7 1.0 8 1.0 9 0.5 10 1.0 11 1.0 12 0.0 13 0.5 14 0.5 15 0.0 16 0.5 17 0.5 18 0.5 19 1.0 20 0.5 21 0.0 22 2.0 23 4.5 24 4.5 25 4.5 26 9.0 27 10.0 28 12.0 29 16.0 30 17.5 31 28.5 32 34.0 33 40.0 34 52.5 35 64.5 36 92.5 37 106.0 38 118.0 39 166.0 40 220.0 41 243.5 42 241.5 43 264.0 44 261.5 45 252.5 46 255.0 47 221.0 48 207.0 49 204.5 50 178.0 51 141.0 52 106.0 53 80.0 54 66.5 55 59.5 56 52.5 57 48.0 58 29.5 59 15.0 60 13.5 61 12.0 62 11.0 63 3.0 64 3.0 65 3.0 66 0.5 67 0.5 68 1.5 69 2.5 70 1.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.5 79 0.5 80 0.0 81 0.0 82 0.0 83 1.0 84 1.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 0.5 91 0.0 92 1.0 93 1.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.06 25-29 0.1 30-34 0.08 35-39 0.06 40-44 0.08 45-49 0.06999999999999999 50-54 0.06 55-59 0.1 60-64 0.06 65-69 0.034999999999999996 70-74 0.1 75-79 0.08499999999999999 80-84 0.05 85-89 0.1 90-94 0.065 95-99 0.06 100-104 0.1 105-109 0.1 110-114 0.06 115-119 0.1 120-124 0.1 125-129 0.09 130-134 0.1 135-139 0.1 140-144 0.06 145-149 0.1 150-151 0.1 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.60000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 70.2292899408284 47.475 2 18.15828402366864 24.55 3 7.581360946745562 15.375 4 2.440828402366864 6.6000000000000005 5 0.9615384615384616 3.25 6 0.4807692307692308 1.95 7 0.07396449704142012 0.35000000000000003 8 0.03698224852071006 0.2 9 0.0 0.0 >10 0.03698224852071006 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTAGCTGATAAGGTCTTTTCTATTGCTGAAATCTATTTAGATGGCAATTA 10 0.25 No Hit GTAGAATTGAGATAAATTGTAATGGAGAAGGGGTGTTATTCGTTGAGGCT 8 0.2 No Hit GATGAGTCATGGTGCCAAGGTCTTGATGGACTCGCCTCCCGCACAGCTGC 7 0.17500000000000002 No Hit GTCCGACTTCACTGCCCCCTTTCAGCCTTTTGGGTCCTGTATCCCAATTC 7 0.17500000000000002 No Hit CATTAATTAGGGCTGAAAGCCCTAACTTAATGGACGGGAGGTATCCCAAT 6 0.15 No Hit CGCCTCTCCAGACACAACAGAGTTAATAATTACAAGAAAAGGAGCTGTCT 6 0.15 No Hit AGGGAGCACTGCATAGCTTATAAGCTTGTAAGAGATGGCTTCCTCCTCTA 6 0.15 No Hit AGGATAACAGGCATCACTGGCTATCAAGACTTCCCAGTATCTACTCTTGA 6 0.15 No Hit TGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAG 6 0.15 No Hit ATGGGAAAGGACTACAACGAGGCCATTGAATCTCTCAAGAAACTCCTCAG 6 0.15 No Hit GATGGAGGGAATACAGGGTATGTAGTGACGCCGCCGCAGATTATGGATGG 6 0.15 No Hit TGTTTATTGAGTGAGTCACTGGAGAAACTCAATGCAGCGAAGAAGGTTTA 6 0.15 No Hit GTTGTTGCAGTTAAAAAGCTCGTAGTTGGACTTTGGGTTGGGTCGGCCGG 6 0.15 No Hit GTTCAAAGACCTGGGATTGGTTCAATGCAACCGCCAAGTCCTACTCAGCC 6 0.15 No Hit CACATTCATACCCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATC 6 0.15 No Hit GATTTGGTGGCAAGAAAGCACCCAAGAAGTCTATAAAGCCTAGTACTCCA 6 0.15 No Hit GAAAGATGAAACCTTGTCCCAATTTGACCACCTTAAGTCCCAGATTTCTG 6 0.15 No Hit GGCATTCGGAAATTGAAAGAAACAAAATTTCTTGTATAACTAGTCATTCA 5 0.125 No Hit GCCGTGGGTTCTGTCATGACCAACAAGTACAGTGAAGGATATCCAGGTGC 5 0.125 No Hit ACCAGAACCGGCAAAACTAGCCATCCCATGGAGGACACGACTGGTCCTTA 5 0.125 No Hit CGCGTCAAGCCGTCAAAAACATCGTACACAGTGCAGAACCCACCGCCTGT 5 0.125 No Hit GTCTGGAACTGATGAAAGAAGTGTTCCAGGCAACACAATTGCAGTTCAAG 5 0.125 No Hit TTAAGTGAGATACTATCATTAAACTTATCACACAACAATCTCACTGGATC 5 0.125 No Hit GCCACCGAAGAAGCTGAAACTGAGGCTCCAGTCGTGGAGCAACCAACTGC 5 0.125 No Hit GGAGGAATGGCGTTGGCAGGACGGTTTATATGTTCAATAACTGGAATTGA 5 0.125 No Hit AGAGATTTGAAGGGAAAGTGGTAATTGTGACCGCTTCAACTCAAGGTATT 5 0.125 No Hit CCGACACCCAATGTTTCAGTTGTTGACCTTGTTGTGAATGTTGAGAAGAA 5 0.125 No Hit GTGGAAGAAGCCGGAGGCCATGTTACCGAGAAGAAAAGGCGCTTAAGTGG 5 0.125 No Hit GGAGGTACATTTCCCGCAAGGGACCCCTGATCGTGTATGGAACTGAAGGT 5 0.125 No Hit CATAATGTTCCTGCTGGTTATTATGGCATTGGGTCACAGATAAGATCCAT 5 0.125 No Hit TGTGTCTCCAAAAGAGACATCAGGGATCACCACTCCTTCGAGTAAACATC 5 0.125 No Hit CACTAGACTTCCACCACCACCGATCTCCTCCACAACTCCTCCTCCACTCC 5 0.125 No Hit GTCTTTTGTTTTTGTTTTTTTGTTTTTTTTTTGTTACATGGGGGGAGGGG 5 0.125 No Hit CTTCTCTCCCTCTATGTGATATGGATCTTGAAGAGTGGGAGCTACTACCC 5 0.125 No Hit GTTAGGTAGCGATGGGAATTTGAGAATGTATAGTGGTGGAACTACAACTA 5 0.125 No Hit GGGAAATTGAGAACAGATCGTCTTTCCTGTTGGCTGTGAAAGCAGATGTA 5 0.125 No Hit GGAAAGGCATGGTACAAGACAATGATCTCAGACAGCGATTACACAGAGTT 5 0.125 No Hit GCAGAACATCCCAGAAAACCAGAGCATGGCATCATCAACTACAAACCCTT 5 0.125 No Hit GTGCTAAAATCTCTCTAGATGGACGTCTTCTCGTTATCTCTTCTATCCCT 5 0.125 No Hit TGAAGGAACAGGACTGCCCTTTGGTTTGAGTAACAACCTTCTTGTTTGGA 5 0.125 No Hit GATGAAAGAATCAAAGAGGTAACTGCACCTGAACTTACATGAAGCCAGGA 5 0.125 No Hit GGCGCAACAATGTTTTGAAGAAAAACTTGGAGCAGCCTTGTTTTCATGTC 5 0.125 No Hit GTCGACGTTATCAAGAAGTGGAAGCTTCAGACCAGAAAACATAGGACAAA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.05 0.0 0.0 0.0 0.0 46-47 0.05 0.0 0.0 0.0 0.0 48-49 0.05 0.0 0.0 0.0 0.0 50-51 0.05 0.0 0.0 0.0 0.0 52-53 0.05 0.0 0.0 0.0 0.0 54-55 0.05 0.0 0.0 0.0 0.0 56-57 0.05 0.0 0.0 0.0 0.0 58-59 0.05 0.0 0.0 0.0 0.0 60-61 0.05 0.0 0.0 0.0 0.0 62-63 0.05 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.05 0.0 0.025 0.0 0.0 68-69 0.05 0.0 0.025 0.0 0.0 70-71 0.05 0.0 0.025 0.0 0.0 72-73 0.0875 0.0 0.025 0.0 0.0 74-75 0.1 0.0 0.025 0.0 0.0 76-77 0.1 0.0 0.025 0.0 0.0 78-79 0.1 0.0 0.025 0.0 0.0 80-81 0.1 0.0 0.025 0.0 0.0 82-83 0.1 0.0 0.025 0.0 0.0 84-85 0.1 0.0 0.025 0.0 0.0 86-87 0.125 0.0 0.025 0.0 0.0 88-89 0.15 0.0 0.025 0.0 0.0 90-91 0.175 0.0 0.025 0.0 0.0 92-93 0.225 0.0 0.025 0.0 0.0 94-95 0.25 0.0 0.025 0.0 0.0 96-97 0.2875 0.0 0.025 0.0 0.0 98-99 0.4 0.0 0.025 0.0 0.0 100-101 0.5375 0.0 0.025 0.0 0.0 102-103 0.7625 0.0 0.025 0.0 0.0 104-105 0.8875 0.0 0.025 0.0 0.0 106-107 0.9375 0.0 0.025 0.0 0.0 108-109 1.025 0.0 0.025 0.0 0.0 110-111 1.3375 0.0 0.025 0.0 0.0 112-113 1.725 0.0 0.025 0.0 0.0 114-115 2.0125 0.0 0.025 0.0 0.0 116-117 2.2 0.0 0.025 0.0 0.0 118-119 2.425 0.0 0.025 0.0 0.0 120-121 2.6500000000000004 0.0 0.025 0.0 0.0 122-123 2.875 0.0 0.025 0.0 0.0 124-125 3.1624999999999996 0.0 0.025 0.0 0.0 126-127 3.6125 0.0 0.025 0.0 0.0 128-129 4.225 0.0 0.025 0.0 0.0 130-131 4.5875 0.0 0.025 0.0 0.0 132-133 5.15 0.0 0.025 0.0 0.0 134-135 5.65 0.0 0.025 0.0 0.0 136-137 6.0 0.0 0.025 0.0 0.0 138-139 6.35 0.0 0.025 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE Read 831997 spots for SRR13695470.sra Written 831997 spots for SRR13695470.sra Read 831997 spots for SRR13695470.sra Written 831997 spots for SRR13695470.sra Read 831997 spots for SRR13695470.sra Written 831997 spots for SRR13695470.sra Read 831997 spots for SRR13695470.sra Written 831997 spots for SRR13695470.sra Read 831997 spots for SRR13695470.sra Written 831997 spots for SRR13695470.sra Read 831997 spots for SRR13695470.sra Written 831997 spots for SRR13695470.sra Read 831997 spots for SRR13695470.sra Written 831997 spots for SRR13695470.sra Read 831997 spots for SRR13695470.sra Written 831997 spots for SRR13695470.sra Read 831997 spots for SRR13695470.sra Written 831997 spots for SRR13695470.sra Read 831997 spots for SRR13695470.sra Written 831997 spots for SRR13695470.sra Read 831997 spots for SRR13695470.sra Written 831997 spots for SRR13695470.sra Read 831997 spots for SRR13695470.sra Written 831997 spots for SRR13695470.sra Read 831997 spots for SRR13695470.sra Written 831997 spots for SRR13695470.sra Read 831997 spots for SRR13695470.sra Written 831997 spots for SRR13695470.sra Read 831998 spots for SRR13695470.sra Written 831998 spots for SRR13695470.sra Read 831997 spots for SRR13695470.sra Written 831997 spots for SRR13695470.sra Read 831997 spots for SRR13695470.sra Written 831997 spots for SRR13695470.sra Read 831997 spots for SRR13695470.sra Written 831997 spots for SRR13695470.sra Read 831997 spots for SRR13695470.sra Written 831997 spots for SRR13695470.sra Read 831997 spots for SRR13695470.sra Written 831997 spots for SRR13695470.sra SRR ids: ['SRR13695470.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_5d5rggjl SRR13695470.sra spots: 16639941 blocks: [[1, 831997], [831998, 1663994], [1663995, 2495991], [2495992, 3327988], [3327989, 4159985], [4159986, 4991982], [4991983, 5823979], [5823980, 6655976], [6655977, 7487973], [7487974, 8319970], [8319971, 9151967], [9151968, 9983964], [9983965, 10815961], [10815962, 11647958], [11647959, 12479955], [12479956, 13311952], [13311953, 14143949], [14143950, 14975946], [14975947, 15807943], [15807944, 16639941]] SRR13695470 file size 5633279 SRR13695470 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695470 SRR13695470_1.fastq SRR13695470_2.fastq Input file: SRR13695470_1.fastq Paired file: SRR13695470_2.fastq trimmed: SRR13695470-trimmed-pair1.fastq, SRR13695470-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 05:37:56 2025 >> started Wed Feb 12 05:38:15 2025 >> done (18.409s) 16639941 read pairs processed; of these: 134 ( 0.00%) short read pairs filtered out after trimming by size control 931 ( 0.01%) empty read pairs filtered out after trimming by size control 16638876 (99.99%) read pairs available; of these: 1568283 ( 9.43%) trimmed read pairs available after processing 15070593 (90.57%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 3 0.00% 19 0 0.00% 20 1 0.00% 21 2 0.00% 22 0 0.00% 23 1 0.00% 24 1 0.00% 25 5 0.00% 26 1 0.00% 27 0 0.00% 28 2 0.00% 29 3 0.00% 30 0 0.00% 31 2 0.00% 32 1 0.00% 33 3 0.00% 34 1 0.00% 35 5 0.00% 36 9 0.00% 37 2 0.00% 38 5 0.00% 39 10 0.00% 40 7 0.00% 41 8 0.00% 42 5 0.00% 43 17 0.00% 44 3 0.00% 45 12 0.00% 46 20 0.00% 47 19 0.00% 48 18 0.00% 49 21 0.00% 50 23 0.00% 51 23 0.00% 52 31 0.00% 53 31 0.00% 54 39 0.00% 55 42 0.00% 56 53 0.00% 57 51 0.00% 58 78 0.00% 59 96 0.00% 60 100 0.00% 61 128 0.00% 62 125 0.00% 63 146 0.00% 64 157 0.00% 65 177 0.00% 66 162 0.00% 67 268 0.00% 68 251 0.00% 69 325 0.00% 70 359 0.00% 71 447 0.00% 72 498 0.00% 73 618 0.00% 74 692 0.00% 75 782 0.00% 76 868 0.01% 77 936 0.01% 78 1040 0.01% 79 1244 0.01% 80 1398 0.01% 81 1577 0.01% 82 1818 0.01% 83 2112 0.01% 84 2513 0.02% 85 2816 0.02% 86 3021 0.02% 87 3121 0.02% 88 3464 0.02% 89 3868 0.02% 90 4238 0.03% 91 4636 0.03% 92 5133 0.03% 93 5792 0.03% 94 5872 0.04% 95 6772 0.04% 96 7132 0.04% 97 7877 0.05% 98 8222 0.05% 99 9000 0.05% 100 9407 0.06% 101 9846 0.06% 102 10535 0.06% 103 11056 0.07% 104 11748 0.07% 105 12809 0.08% 106 13619 0.08% 107 14079 0.08% 108 14493 0.09% 109 14972 0.09% 110 15893 0.10% 111 16789 0.10% 112 17059 0.10% 113 18132 0.11% 114 18833 0.11% 115 20128 0.12% 116 21041 0.13% 117 21535 0.13% 118 22750 0.14% 119 23598 0.14% 120 24352 0.15% 121 24721 0.15% 122 26058 0.16% 123 26222 0.16% 124 27529 0.17% 125 27921 0.17% 126 29959 0.18% 127 30427 0.18% 128 30829 0.19% 129 31577 0.19% 130 33212 0.20% 131 33117 0.20% 132 33925 0.20% 133 35671 0.21% 134 35822 0.22% 135 37187 0.22% 136 37898 0.23% 137 38799 0.23% 138 39603 0.24% 139 41074 0.25% 140 41492 0.25% 141 42297 0.25% 142 43133 0.26% 143 43852 0.26% 144 44616 0.27% 145 45935 0.28% 146 46243 0.28% 147 47026 0.28% 148 48747 0.29% 149 49681 0.30% 150 50677 0.30% 151 15070593 90.57% 16638876 reads passed initial QC criterion=sequence-density sequence-density=0.41 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=22 prefix-density=0.41 prefix-fanout=1.9 sequence=CGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTTCGAGCGAAGAAGGT criterion=fanout-score sequence-density=0.01 sequence-density-rank=27 fanout-score=514.39 fanout-score-rank=1 prefix-density=0.27 prefix-fanout=18.1 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAG criterion=sequence-density sequence-density=0.97 sequence-density-rank=1 fanout-score=2.03 fanout-score-rank=30 prefix-density=0.97 prefix-fanout=2.0 sequence=CTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAGTGAACAAACAAAAGTGTCCGTGAGTGTCTTTATCTTTGTGTCTTCTTCA criterion=fanout-score sequence-density=0.14 sequence-density-rank=27 fanout-score=22.10 fanout-score-rank=1 prefix-density=0.35 prefix-fanout=8.4 sequence=CAAGGAAAATCCTTCCAGTGTGAACTTGTCTTTGCCAAGATGGGAATTAACCCAATCATGATGAGTGCTGGAGAATTGGAAAGTGGGAACGCTGGTGAACCCGCAAAGCTTATCAGGCAAAGGTACCGTGAGGCGGCTGATATAATCAAGAAGAAGGGAAAGATGTGCTGCCTCTTCATCAACGATCTTGATGC SRR13695470 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 05:39:21 Started mapping on | Feb 12 05:39:21 Finished on | Feb 12 05:41:05 Mapping speed, Million of reads per hour | 575.96 Number of input reads | 16638876 Average input read length | 289 UNIQUE READS: Uniquely mapped reads number | 14275530 Uniquely mapped reads % | 85.80% Average mapped length | 289.57 Number of splices: Total | 14075513 Number of splices: Annotated (sjdb) | 13780891 Number of splices: GT/AG | 13787856 Number of splices: GC/AG | 230446 Number of splices: AT/AC | 7353 Number of splices: Non-canonical | 49858 Mismatch rate per base, % | 0.41% Deletion rate per base | 0.03% Deletion average length | 2.82 Insertion rate per base | 0.02% Insertion average length | 2.45 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 365365 % of reads mapped to multiple loci | 2.20% Number of reads mapped to too many loci | 165682 % of reads mapped to too many loci | 1.00% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 10.84% % of reads unmapped: other | 0.17% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1998339 1998339 1998339 N_multimapping 365365 365365 365365 N_noFeature 656788 13950484 834008 N_ambiguous 268146 3043 117943 UnstrandedReadsAssigned:13350596 PositiveStrandReadsAssigned:322003 NegativeStrandReadsAssigned:13323579 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695470 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695470-trimmed-pair1.fastq SRR13695470-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 16,638,876 reads, 14,517,847 reads pseudoaligned [quant] estimated average fragment length: 247.241 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,071 rounds 52401 SRR13695470.ke.tsv 34699 SRR13695470.se.tsv 87100 total ==> SRR13695470.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1771.76 525 18.4289 Potri.005G024800.1.v4.1 1035 788.759 279 21.9991 Potri.004G059700.1.v4.1 961 714.878 0 0 Potri.007G009000.2.v4.1 1416 1169.76 0 0 Potri.003G141000.2.v4.1 2943 2696.76 945 21.7939 Potri.016G087400.1.v4.1 270 85.5542 817 593.916 Potri.015G069301.1.v4.1 564 327.023 0 0 Potri.010G195200.1.v4.1 1773 1526.76 77.9422 3.17502 Potri.012G127500.1.v4.1 977 730.806 73 6.21248 ==> SRR13695470.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 111 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 186 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 7 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 3 SRR13695470 completed mapping pipeline successfully