Starting /dee2/code/volunteer_pipeline.sh SRR13695471 current disk space = 3049943666688 free memory = 1582705560 SRR13695471 SRAfilesize 877d681326a6ab5597465ea6d0a7b147 SRR13695471.sra SRR13695471.sra file validated SRR13695471 is paired end SRR13695471 is conventional basespace SRR13695471 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695471_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.54 37.0 37.0 37.0 37.0 37.0 2 36.24 37.0 37.0 37.0 37.0 37.0 3 36.527 37.0 37.0 37.0 37.0 37.0 4 36.5545 37.0 37.0 37.0 37.0 37.0 5 36.5495 37.0 37.0 37.0 37.0 37.0 6 36.529 37.0 37.0 37.0 37.0 37.0 7 36.3695 37.0 37.0 37.0 37.0 37.0 8 36.4755 37.0 37.0 37.0 37.0 37.0 9 36.5325 37.0 37.0 37.0 37.0 37.0 10-14 36.558 37.0 37.0 37.0 37.0 37.0 15-19 36.5202 37.0 37.0 37.0 37.0 37.0 20-24 36.4431 37.0 37.0 37.0 37.0 37.0 25-29 36.372699999999995 37.0 37.0 37.0 37.0 37.0 30-34 36.395599999999995 37.0 37.0 37.0 37.0 37.0 35-39 36.3868 37.0 37.0 37.0 37.0 37.0 40-44 36.352 37.0 37.0 37.0 37.0 37.0 45-49 36.3183 37.0 37.0 37.0 37.0 37.0 50-54 36.3151 37.0 37.0 37.0 37.0 37.0 55-59 36.32770000000001 37.0 37.0 37.0 37.0 37.0 60-64 36.2864 37.0 37.0 37.0 37.0 37.0 65-69 36.290400000000005 37.0 37.0 37.0 37.0 37.0 70-74 36.2743 37.0 37.0 37.0 37.0 37.0 75-79 36.191500000000005 37.0 37.0 37.0 37.0 37.0 80-84 36.1509 37.0 37.0 37.0 37.0 37.0 85-89 36.1271 37.0 37.0 37.0 37.0 37.0 90-94 36.13380000000001 37.0 37.0 37.0 37.0 37.0 95-99 36.0728 37.0 37.0 37.0 37.0 37.0 100-104 36.061400000000006 37.0 37.0 37.0 37.0 37.0 105-109 36.0958 37.0 37.0 37.0 37.0 37.0 110-114 35.963899999999995 37.0 37.0 37.0 37.0 37.0 115-119 36.007999999999996 37.0 37.0 37.0 37.0 37.0 120-124 35.9628 37.0 37.0 37.0 37.0 37.0 125-129 35.9395 37.0 37.0 37.0 37.0 37.0 130-134 35.9474 37.0 37.0 37.0 37.0 37.0 135-139 35.8814 37.0 37.0 37.0 37.0 37.0 140-144 35.740700000000004 37.0 37.0 37.0 37.0 37.0 145-149 35.6101 37.0 37.0 37.0 37.0 37.0 150-151 35.34075 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 24 1.0 25 5.0 26 5.0 27 13.0 28 14.0 29 26.0 30 25.0 31 37.0 32 56.0 33 80.0 34 122.0 35 365.0 36 2954.0 37 297.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.800000000000004 12.275 7.124999999999999 40.8 2 18.97590361445783 13.654618473895583 38.83032128514056 28.539156626506024 3 17.925 17.075000000000003 28.675 36.325 4 23.45 25.95 20.674999999999997 29.925 5 23.35 32.5 24.925 19.225 6 20.424999999999997 34.65 23.075000000000003 21.85 7 13.575000000000001 27.175 42.075 17.175 8 17.025000000000002 26.325 32.425 24.224999999999998 9 17.5 22.625 36.375 23.5 10-14 19.24 29.465000000000003 28.725 22.57 15-19 19.075 28.299999999999997 27.88 24.745 20-24 19.495 29.049999999999997 27.43 24.025 25-29 19.625 28.71 27.66 24.005000000000003 30-34 19.37 29.09 27.73 23.810000000000002 35-39 20.16 28.000000000000004 27.62 24.22 40-44 20.415 28.915000000000003 27.16 23.51 45-49 19.685 29.025000000000002 27.944999999999997 23.345 50-54 20.02 28.494999999999997 27.49 23.995 55-59 20.585 28.715000000000003 27.525 23.175 60-64 20.315 28.485 28.389999999999997 22.81 65-69 20.73 28.595 28.29 22.384999999999998 70-74 20.599999999999998 28.804999999999996 27.26 23.335 75-79 20.13 28.235 28.000000000000004 23.635 80-84 20.405 29.24 27.6 22.755 85-89 19.415 28.825 27.73 24.03 90-94 20.549999999999997 27.99 27.58 23.880000000000003 95-99 20.4 27.755000000000003 28.025 23.82 100-104 20.205000000000002 29.525000000000002 27.205000000000002 23.064999999999998 105-109 20.674999999999997 28.799999999999997 27.57 22.955000000000002 110-114 20.14 28.77 27.555000000000003 23.535 115-119 20.47 27.99 27.634999999999998 23.905 120-124 21.15 27.975 26.840000000000003 24.035 125-129 20.535 28.625 27.134999999999998 23.705000000000002 130-134 20.31 28.515 27.485 23.69 135-139 21.015 27.82 27.54 23.625 140-144 20.76 28.59 27.26 23.39 145-149 20.23 29.585 26.71 23.474999999999998 150-151 20.375 28.549999999999997 26.35 24.725 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.5 18 2.0 19 1.5 20 2.0 21 5.0 22 5.0 23 3.5 24 2.0 25 2.5 26 5.5 27 8.0 28 16.5 29 16.5 30 12.5 31 23.0 32 29.5 33 39.0 34 67.0 35 83.5 36 88.0 37 92.5 38 111.5 39 143.5 40 181.5 41 219.0 42 262.5 43 271.0 44 268.5 45 298.5 46 288.5 47 248.5 48 210.0 49 175.0 50 152.5 51 153.5 52 132.0 53 99.0 54 76.0 55 50.5 56 37.5 57 25.5 58 24.0 59 26.0 60 14.5 61 6.0 62 6.0 63 5.5 64 2.5 65 1.0 66 0.5 67 1.5 68 1.5 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.4 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.1 #Duplication Level Percentage of deduplicated Percentage of total 1 71.64764621968617 50.224999999999994 2 19.293865905848786 27.05 3 5.741797432239657 12.075 4 2.1398002853067046 6.0 5 0.7132667617689016 2.5 6 0.24964336661911554 1.05 7 0.14265335235378032 0.7000000000000001 8 0.07132667617689016 0.4 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGAGCAATTCACCTTCAAGCTTTACCTCTCTCTCCTCCAATTCTCTTACT 8 0.2 No Hit CAACAATTGAACAAGATTCTCATGATGCAAAGATTTCAGGTTGAAGATTT 8 0.2 No Hit GTTTAATAAAGAAAGAAACACTTGGCTCTCTGCACAGAAGATTACGAGTT 7 0.17500000000000002 No Hit GTTCTGGTGTTTCTGAATAGAATGAAATGAGATTGTTTCGCATATCAATG 7 0.17500000000000002 No Hit ATCAACTAGGTCAGTGACATCGATATAGTCGCGTACACATGTGCCATCAG 7 0.17500000000000002 No Hit GTTGAAACATATATTGCTAGAGCATAACATTCAAAAGAAGCTGAATAATC 7 0.17500000000000002 No Hit AGCCAATCCAGCTACCCTTTTGACTAAGTTTAGATTGTTCTGTCTCTGAC 6 0.15 No Hit TGTGGGTCCGCTAAATGCCAACACATCAGCGAATTTTGTGCTGACCTTTG 6 0.15 No Hit GTGGTCTAAACATCCTTGACAACAAGATCACCGTTGACAAACTCTCTGTA 6 0.15 No Hit CCTCAGGAGGCTGGACCAGCTTCCGGTTGTGTGGTGCTGTCATCTCCTTC 6 0.15 No Hit GTACTCTTCTAAAGAAAGTGTTACACTGGTAGGTGTATCCACATCACTGG 6 0.15 No Hit CTTGGGGATAACTTTGCCTTTTTCTTGTCTTATTATGTTCCAGAACTCGT 6 0.15 No Hit GTGGATGTTTGTTGTCGCCCCCCACCCCCAACATCGATGGTGTCAGGAAG 6 0.15 No Hit GTAGCACCAGTAATCATGTTCTTAACATAATCAGCATGACCAGGACAATC 5 0.125 No Hit CTTGAACAATATATCACAGGAAACTTCACTAAAAATTGACCATGAAAAAA 5 0.125 No Hit GTGCGGAAAGATGACGGCGGATGCAGAAGGTGGAGCTCCAGAGGTTACTC 5 0.125 No Hit CAAACAAGGTTTCCTTCACCCACGGTCAATGCCTCTTTCCTTCTCATGCT 5 0.125 No Hit CAGAAATCAAAAGGGGATCCACACTGAACTAAGATCGCCACTCAAGAGGA 5 0.125 No Hit GTTCGGCGCTTCATATCCATAAACATCAGAGACAAGAAGAATGGCAAGTT 5 0.125 No Hit GTTCTGCCAAGGATCCATGCTCCATGTACTTGTATACCAAAAGCCTGTGC 5 0.125 No Hit GTGGATTTATGAGAGGTTGAGAGAGTATTCGGAGGAGAGGGGAGTGCTCG 5 0.125 No Hit CTTTAAAAAAAAATAAACATTTGTTGTCCATCCCCTTAAGAAATTAAAAA 5 0.125 No Hit GCTAGATTTCATAAACTTCTTCAGCAAAAGAACCACGATTGCAATGGCGA 5 0.125 No Hit CTTAGAAAGTGCTTCTCCTTACACACCCACAAGCCTAGAAAAGGAATTAG 5 0.125 No Hit TATATTTCTTCAAGTTCCTCTTTGCTCGAAATTGACCAAGTTTGTCTCTC 5 0.125 No Hit CTTTACCTTTTTCTTCAACCACAAAAGCTGACACAGGGAAAGTCCTGTAG 5 0.125 No Hit CGCTAATCGCCAGTGTCAAACGAGCCTTCCTTTCGGTCCTACTCCTATGA 5 0.125 No Hit CGACATCTTTAAATAAAAAGGCATGCTCTCAACTTCCCAGAGTAAGTTAT 5 0.125 No Hit GGTGTCTCCAAGGGCATGGACGTGGAAGCCATGAAGGCCTGGCTTAAGAC 5 0.125 No Hit CTCAAGTCCATTCCAGATCCACCAATCCATATGTATTCAGCAATGATCTT 5 0.125 No Hit TCCACGATTAGTTCATCAACTCCCCAGTCTTCATAACTTCCATCAGGCAA 5 0.125 No Hit ACCTGATGCAGCCAATCCACCAAGTCCAAGCCCAACGATCACCCCTTTGG 5 0.125 No Hit CCCAAAATTCCATTCTGCACAGACATAAGGTCCAATGCGGAGATGAACAT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.2 0.0 0.0 0.0 0.0 84-85 0.225 0.0 0.0 0.0 0.0 86-87 0.25 0.0 0.0 0.0 0.0 88-89 0.2625 0.0 0.0 0.0 0.0 90-91 0.3125 0.0 0.0 0.0 0.0 92-93 0.375 0.0 0.0 0.0 0.0 94-95 0.3875 0.0 0.0 0.0 0.0 96-97 0.475 0.0 0.0 0.0 0.0 98-99 0.55 0.0 0.0 0.0 0.0 100-101 0.7625 0.0 0.0 0.0 0.0 102-103 0.8500000000000001 0.0 0.0 0.0 0.0 104-105 1.0625 0.0 0.0 0.0 0.0 106-107 1.175 0.0 0.0 0.0 0.0 108-109 1.35 0.0 0.0 0.0 0.0 110-111 1.5625 0.0 0.0 0.0 0.0 112-113 1.75 0.0 0.0 0.0 0.0 114-115 1.9625 0.0 0.0 0.0 0.0 116-117 2.2625 0.0 0.0 0.0 0.0 118-119 2.4875 0.0 0.0 0.0 0.0 120-121 2.6875 0.0 0.0 0.0 0.0 122-123 2.95 0.0 0.0 0.0 0.0 124-125 3.175 0.0 0.0 0.0 0.0 126-127 3.4 0.0 0.0 0.0 0.0 128-129 3.7875 0.0 0.0 0.0 0.0 130-131 3.9124999999999996 0.0 0.0 0.0 0.0 132-133 4.2125 0.0 0.0 0.0 0.0 134-135 4.75 0.0 0.0 0.0 0.0 136-137 5.3125 0.0 0.0 0.0 0.0 138-139 5.7625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTTCCGG 10 0.006830828 145.0 7 TTCCGGC 10 0.006830828 145.0 8 GTTTCCG 10 0.006830828 145.0 6 TCCGGCA 10 0.006830828 145.0 9 CGGTTTC 10 0.006830828 145.0 4 CCGGTTT 10 0.006830828 145.0 3 >>END_MODULE SRR13695471 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695471_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.10225 37.0 37.0 37.0 37.0 37.0 2 36.249 37.0 37.0 37.0 37.0 37.0 3 36.172 37.0 37.0 37.0 37.0 37.0 4 36.344 37.0 37.0 37.0 37.0 37.0 5 36.254 37.0 37.0 37.0 37.0 37.0 6 36.2645 37.0 37.0 37.0 37.0 37.0 7 36.3315 37.0 37.0 37.0 37.0 37.0 8 36.371 37.0 37.0 37.0 37.0 37.0 9 36.283 37.0 37.0 37.0 37.0 37.0 10-14 36.2568 37.0 37.0 37.0 37.0 37.0 15-19 36.2444 37.0 37.0 37.0 37.0 37.0 20-24 36.2452 37.0 37.0 37.0 37.0 37.0 25-29 36.1892 37.0 37.0 37.0 37.0 37.0 30-34 36.16245 37.0 37.0 37.0 37.0 37.0 35-39 36.0524 37.0 37.0 37.0 37.0 37.0 40-44 36.1178 37.0 37.0 37.0 37.0 37.0 45-49 36.0893 37.0 37.0 37.0 37.0 37.0 50-54 36.0273 37.0 37.0 37.0 37.0 37.0 55-59 36.03 37.0 37.0 37.0 37.0 37.0 60-64 36.005100000000006 37.0 37.0 37.0 37.0 37.0 65-69 35.96685 37.0 37.0 37.0 37.0 37.0 70-74 35.924600000000005 37.0 37.0 37.0 37.0 37.0 75-79 35.9146 37.0 37.0 37.0 37.0 37.0 80-84 35.92385 37.0 37.0 37.0 37.0 37.0 85-89 35.8089 37.0 37.0 37.0 37.0 37.0 90-94 35.83165 37.0 37.0 37.0 37.0 37.0 95-99 35.80775 37.0 37.0 37.0 37.0 37.0 100-104 35.763099999999994 37.0 37.0 37.0 37.0 37.0 105-109 35.775400000000005 37.0 37.0 37.0 37.0 37.0 110-114 35.6712 37.0 37.0 37.0 37.0 37.0 115-119 35.6658 37.0 37.0 37.0 37.0 37.0 120-124 35.661 37.0 37.0 37.0 37.0 37.0 125-129 35.59875 37.0 37.0 37.0 37.0 37.0 130-134 35.48855 37.0 37.0 37.0 37.0 37.0 135-139 35.57860000000001 37.0 37.0 37.0 37.0 37.0 140-144 35.445100000000004 37.0 37.0 37.0 37.0 37.0 145-149 35.3309 37.0 37.0 37.0 32.2 37.0 150-151 35.054625 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 3.0 14 1.0 15 6.0 16 1.0 17 0.0 18 0.0 19 1.0 20 2.0 21 0.0 22 4.0 23 2.0 24 1.0 25 8.0 26 11.0 27 13.0 28 9.0 29 22.0 30 30.0 31 40.0 32 68.0 33 118.0 34 182.0 35 545.0 36 2691.0 37 241.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 39.70920030082728 22.286287290047632 10.629230383554775 27.375282025570318 2 27.450000000000003 23.95 32.725 15.875 3 22.175 26.85 30.0 20.974999999999998 4 25.45 33.175 22.55 18.825 5 24.224999999999998 37.35 22.175 16.25 6 20.349999999999998 38.975 23.625 17.05 7 20.075000000000003 21.775 38.5 19.650000000000002 8 20.925 25.2 29.45 24.425 9 21.4 26.224999999999998 30.85 21.525 10-14 23.445 29.085 26.93 20.54 15-19 22.38 28.17 28.799999999999997 20.65 20-24 23.121936580974292 28.763629088726617 27.293187956386916 20.821246373912174 25-29 22.576288144072034 27.72886443221611 28.499249624812407 21.19559779889945 30-34 22.700215096793556 29.503276474413486 27.10719823920764 20.689310189585314 35-39 22.58177453235971 28.048414524357305 28.258477543262977 21.111333400020005 40-44 23.07923169267707 27.761104441776713 28.666466586634655 20.493197278911566 45-49 22.398959583833534 27.43097238895558 29.081632653061224 21.08843537414966 50-54 23.56707012103631 28.183455036510953 27.478243473041914 20.771231369410824 55-59 23.39669834917459 27.668834417208604 27.963981990995496 20.97048524262131 60-64 22.64179253776133 27.758327498249475 28.543563068920676 21.05631689506852 65-69 22.91072768192048 28.012003000750184 27.236809202300577 21.840460115028755 70-74 22.736368184092047 28.019009504752372 27.54377188594297 21.700850425212607 75-79 23.216608304152075 27.48874437218609 27.71385692846423 21.5807903951976 80-84 22.93573393348337 27.926981745436358 27.82195548887222 21.315328832208053 85-89 23.001500750375186 28.734367183591797 27.2736368184092 20.990495247623812 90-94 23.478217376081627 27.924773670784774 27.95478417446106 20.642224778672535 95-99 22.50287600660231 28.615015255339372 27.61466513279648 21.26744360526184 100-104 23.736868434217108 26.663331665832917 28.849424712356175 20.750375187593797 105-109 23.2016008004002 27.473736868434216 28.494247123561784 20.830415207603803 110-114 23.221966589976994 27.853356006802038 28.64359307792338 20.281084325297588 115-119 23.676838419209606 27.24862431215608 28.029014507253624 21.045522761380692 120-124 24.607303651825912 27.893946973486745 26.7983991995998 20.700350175087546 125-129 24.34095342904307 27.922565154319447 27.262268020609277 20.47421339602821 130-134 24.283355845715143 27.905347941367754 27.890339686827755 19.92095652608935 135-139 24.082041020510257 28.174087043521762 26.97848924462231 20.765382691345675 140-144 24.25227568270481 27.068120436130837 27.89336801040312 20.786235870761228 145-149 24.732366183091546 28.479239619809903 27.333666833416707 19.45472736368184 150-151 24.853033145716072 27.70481550969356 27.31707317073171 20.12507817385866 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.5 9 0.5 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.5 16 0.5 17 0.0 18 0.0 19 0.0 20 1.0 21 1.0 22 3.0 23 3.5 24 3.0 25 4.0 26 8.5 27 11.5 28 10.0 29 12.5 30 20.0 31 21.0 32 20.5 33 42.0 34 54.5 35 71.0 36 85.0 37 96.5 38 140.5 39 173.0 40 206.0 41 222.0 42 229.5 43 266.5 44 285.5 45 271.0 46 269.5 47 257.5 48 226.0 49 183.0 50 154.0 51 140.0 52 105.0 53 90.5 54 73.0 55 62.5 56 56.0 57 34.0 58 19.0 59 12.0 60 15.0 61 12.5 62 6.0 63 7.5 64 5.5 65 1.0 66 0.0 67 0.5 68 0.5 69 1.0 70 1.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27499999999999997 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.03 25-29 0.05 30-34 0.045 35-39 0.03 40-44 0.04 45-49 0.04 50-54 0.03 55-59 0.05 60-64 0.03 65-69 0.025 70-74 0.05 75-79 0.05 80-84 0.025 85-89 0.05 90-94 0.034999999999999996 95-99 0.034999999999999996 100-104 0.05 105-109 0.05 110-114 0.03 115-119 0.05 120-124 0.05 125-129 0.045 130-134 0.055 135-139 0.05 140-144 0.03 145-149 0.05 150-151 0.0625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.05 #Duplication Level Percentage of deduplicated Percentage of total 1 71.87723054960742 50.349999999999994 2 18.843683083511777 26.400000000000002 3 5.924339757316202 12.45 4 2.212705210563883 6.2 5 0.6423982869379015 2.25 6 0.21413276231263384 0.8999999999999999 7 0.21413276231263384 1.05 8 0.07137758743754462 0.4 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GGGGACGCTTTGGAATAGTATACAAGGCTGAATTGCCGAACGAGATAAAA 8 0.2 No Hit ATCGATTATCCATTACCTGGGGAAAAGTTTGACCAAGCAGAGAAGGACAA 8 0.2 No Hit GGCAATAGTCGAGTGGTTTCTGAGAAGAAAGCAAAGGCATGGTGTGCTGC 7 0.17500000000000002 No Hit GGAAAACACTGGACTTGAAGGTCAGATTCCTCCTACCCTCTTCGATCTTC 7 0.17500000000000002 No Hit CTTATGGCGATGGACGCTGGAACCCATCAACGATTATTCAGCTTCTTTTG 7 0.17500000000000002 No Hit CTCAATCACAGCCATTGCGCCATAACTTCTTTAGTCCCTTCACAGTTCAT 7 0.17500000000000002 No Hit ATGTTATCTGATCATAGAATAGCAGCCGTTAAGCGACTGAATGATGCTTA 7 0.17500000000000002 No Hit ATGGCTGTCATGATCCTAAGATACTTCAATGTTATTGGGTCTGACCCGGA 7 0.17500000000000002 No Hit TATCAGTTAAAGCTGTTCTGTGATAAAGATTACCCGGAGAAGCCACCAAG 6 0.15 No Hit CTTGTTTTCTGCTACGTATGTGCCACGACTACGTGGCAGTGCTAGCAAAC 6 0.15 No Hit GATGATGCCATGGTGGAGAAAAACGTGTTAGATCCATCAGCACCTTGCAT 6 0.15 No Hit GAGTAGATTACTTGCAGCTCAAAAGGAGATAGAAGCTTCCAGGGCCTCTG 6 0.15 No Hit GAGTTACTTGATTGCTATAAGTGACGAGAAGAAGCCAGCAATGAAGGAGA 6 0.15 No Hit CAACAGTAGCATACCTTTTTCTAGTGATCTTAAAGATACTACTCTTCGTG 6 0.15 No Hit CCACGCCTTCCTTTCTCTACCACTCACCTCTCCTCTCCTCTTGATTATTT 5 0.125 No Hit CCCGCATACTCTTCCAAAAATGGTGTGTTTTCCATCTAGAGATGGAGTCG 5 0.125 No Hit CTCACTTTCTTAAAAAAAAAAAATAACAATAAAATGCAAACGCTACTCCT 5 0.125 No Hit AAGCCAGCCTCCCCTTCTCAATTAATAAGCGAAGCCCCTCCTCCTCTAGG 5 0.125 No Hit CATCCACCCCTACTTTCTCCCTAACACCCACCTCGAAACTCCCCTCAAAA 5 0.125 No Hit GTTTTATCACCTCTACAATCAGTTGGCTACCAATACCTTGGAGAGGGTTG 5 0.125 No Hit GGGGAACCAATTGAAACTGAAGACAAAGAGGAACAGGAACCAGTGGAGCG 5 0.125 No Hit GAATAATGGTGAAGGCTGTAGCTGTTCTTAATAGCAGTGAAGGTGTGAGT 5 0.125 No Hit TGTGAACCTGCTTTTGCTGCGCAACAAATCGCTGAAATAGCAGAAGGTGA 5 0.125 No Hit GAGGCTGTAACTGCTGCTGAAGACCTTCCAAATCCAGCTGTTGGTGAAAT 5 0.125 No Hit GTCTTTTGCCCTTGCACGATATTGTAGAGTGCGGGATTGTGAGGGAGACA 5 0.125 No Hit GTTGAATCAAGAAAAAATGGCAGGGCCTCAGTGCTGTGCTCACCCACCAA 5 0.125 No Hit TTTAGATTTAGATAAAGTGATTAGTACTGAGGTTTCGAGGCCAAAGAAAT 5 0.125 No Hit GGTGTACTCACTGCTGAGGATGCAAGGCTTTCAGTTCAAGCTGGAGCAGC 5 0.125 No Hit AGTTCTTAACCCTCTTCTTGATGGTCTTGATTGTTGGTTCTAAGCTGATC 5 0.125 No Hit GTGGAATTGAAGATGAGCTGCTTCAGCCTCAATTTAGTATTGGGTTGAAG 5 0.125 No Hit AGGAAGACAAAGCAAGACATAAACCTACCAGTGATTCAATCAAGCACAAG 5 0.125 No Hit GCAAAGGTAGATTATTGATAATAAATGGTGAAATTGCATTATTTTAGGTT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.025 0.0 0.0 0.0 0.0 76-77 0.025 0.0 0.0 0.0 0.0 78-79 0.025 0.0 0.0 0.0 0.0 80-81 0.025 0.0 0.0 0.0 0.0 82-83 0.2 0.0 0.0 0.0 0.0 84-85 0.225 0.0 0.0 0.0 0.0 86-87 0.2625 0.0 0.0 0.0 0.0 88-89 0.2875 0.0 0.0 0.0 0.0 90-91 0.3375 0.0 0.0 0.0 0.0 92-93 0.4 0.0 0.0 0.0 0.0 94-95 0.4125 0.0 0.0 0.0 0.0 96-97 0.5 0.0 0.0 0.0 0.0 98-99 0.575 0.0 0.0 0.0 0.0 100-101 0.7875 0.0 0.0 0.0 0.0 102-103 0.875 0.0 0.0 0.0 0.0 104-105 1.0875 0.0 0.0 0.0 0.0 106-107 1.2 0.0 0.0 0.0 0.0 108-109 1.375 0.0 0.0 0.0 0.0 110-111 1.5875 0.0 0.0 0.0 0.0 112-113 1.775 0.0 0.0 0.0 0.0 114-115 1.9875 0.0 0.0 0.0 0.0 116-117 2.2875 0.0 0.0 0.0 0.0 118-119 2.5125 0.0 0.0 0.0 0.0 120-121 2.7125 0.0 0.0 0.0 0.0 122-123 2.9749999999999996 0.0 0.0 0.0 0.0 124-125 3.225 0.0 0.0 0.0 0.0 126-127 3.45 0.0 0.0 0.0 0.0 128-129 3.8375 0.0 0.0 0.0 0.0 130-131 3.9625000000000004 0.0 0.0 0.0 0.0 132-133 4.2625 0.0 0.0 0.0 0.0 134-135 4.8125 0.0 0.0 0.0 0.0 136-137 5.375 0.0 0.0 0.0 0.0 138-139 5.8125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTATT 10 0.006830828 145.0 5 CCGCCGT 10 0.006830828 145.0 1 CCGTTAT 10 0.006830828 145.0 4 GCCGTTA 10 0.006830828 145.0 3 AAAATCA 10 0.006830828 145.0 4 CGCCGTT 10 0.006830828 145.0 2 >>END_MODULE Read 786015 spots for SRR13695471.sra Written 786015 spots for SRR13695471.sra Read 786015 spots for SRR13695471.sra Written 786015 spots for SRR13695471.sra Read 786015 spots for SRR13695471.sra Written 786015 spots for SRR13695471.sra Read 786015 spots for SRR13695471.sra Written 786015 spots for SRR13695471.sra Read 786015 spots for SRR13695471.sra Written 786015 spots for SRR13695471.sra Read 786015 spots for SRR13695471.sra Written 786015 spots for SRR13695471.sra Read 786015 spots for SRR13695471.sra Written 786015 spots for SRR13695471.sra Read 786015 spots for SRR13695471.sra Written 786015 spots for SRR13695471.sra Read 786015 spots for SRR13695471.sra Written 786015 spots for SRR13695471.sra Read 786015 spots for SRR13695471.sra Written 786015 spots for SRR13695471.sra Read 786015 spots for SRR13695471.sra Written 786015 spots for SRR13695471.sra Read 786015 spots for SRR13695471.sra Written 786015 spots for SRR13695471.sra Read 786015 spots for SRR13695471.sra Written 786015 spots for SRR13695471.sra Read 786015 spots for SRR13695471.sra Written 786015 spots for SRR13695471.sra Read 786015 spots for SRR13695471.sra Written 786015 spots for SRR13695471.sra Read 786015 spots for SRR13695471.sra Written 786015 spots for SRR13695471.sra Read 786015 spots for SRR13695471.sra Written 786015 spots for SRR13695471.sra Read 786015 spots for SRR13695471.sra Written 786015 spots for SRR13695471.sra Read 786015 spots for SRR13695471.sra Written 786015 spots for SRR13695471.sra Read 786033 spots for SRR13695471.sra Written 786033 spots for SRR13695471.sra SRR ids: ['SRR13695471.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_3g3t4pfb SRR13695471.sra spots: 15720318 blocks: [[1, 786015], [786016, 1572030], [1572031, 2358045], [2358046, 3144060], [3144061, 3930075], [3930076, 4716090], [4716091, 5502105], [5502106, 6288120], [6288121, 7074135], [7074136, 7860150], [7860151, 8646165], [8646166, 9432180], [9432181, 10218195], [10218196, 11004210], [11004211, 11790225], [11790226, 12576240], [12576241, 13362255], [13362256, 14148270], [14148271, 14934285], [14934286, 15720318]] SRR13695471 file size 5320751 SRR13695471 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695471 SRR13695471_1.fastq SRR13695471_2.fastq Input file: SRR13695471_1.fastq Paired file: SRR13695471_2.fastq trimmed: SRR13695471-trimmed-pair1.fastq, SRR13695471-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 06:02:54 2025 >> started Wed Feb 12 06:03:11 2025 >> done (17.048s) 15720318 read pairs processed; of these: 104 ( 0.00%) short read pairs filtered out after trimming by size control 689 ( 0.00%) empty read pairs filtered out after trimming by size control 15719525 (99.99%) read pairs available; of these: 1424785 ( 9.06%) trimmed read pairs available after processing 14294740 (90.94%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 2 0.00% 20 1 0.00% 21 0 0.00% 22 2 0.00% 23 5 0.00% 24 4 0.00% 25 5 0.00% 26 2 0.00% 27 2 0.00% 28 1 0.00% 29 3 0.00% 30 1 0.00% 31 1 0.00% 32 4 0.00% 33 6 0.00% 34 8 0.00% 35 4 0.00% 36 4 0.00% 37 7 0.00% 38 5 0.00% 39 6 0.00% 40 4 0.00% 41 6 0.00% 42 8 0.00% 43 10 0.00% 44 12 0.00% 45 15 0.00% 46 17 0.00% 47 13 0.00% 48 27 0.00% 49 30 0.00% 50 43 0.00% 51 19 0.00% 52 46 0.00% 53 31 0.00% 54 53 0.00% 55 50 0.00% 56 69 0.00% 57 81 0.00% 58 67 0.00% 59 103 0.00% 60 120 0.00% 61 142 0.00% 62 162 0.00% 63 181 0.00% 64 206 0.00% 65 213 0.00% 66 302 0.00% 67 293 0.00% 68 378 0.00% 69 408 0.00% 70 448 0.00% 71 577 0.00% 72 644 0.00% 73 712 0.00% 74 821 0.01% 75 886 0.01% 76 982 0.01% 77 1198 0.01% 78 1243 0.01% 79 1387 0.01% 80 1651 0.01% 81 1839 0.01% 82 2057 0.01% 83 2280 0.01% 84 2664 0.02% 85 2930 0.02% 86 3209 0.02% 87 3280 0.02% 88 3785 0.02% 89 4058 0.03% 90 4518 0.03% 91 4842 0.03% 92 5040 0.03% 93 5837 0.04% 94 6197 0.04% 95 6679 0.04% 96 7322 0.05% 97 7755 0.05% 98 8241 0.05% 99 8561 0.05% 100 9051 0.06% 101 9428 0.06% 102 10202 0.06% 103 10858 0.07% 104 11769 0.07% 105 12072 0.08% 106 12548 0.08% 107 13185 0.08% 108 13815 0.09% 109 14517 0.09% 110 15031 0.10% 111 15677 0.10% 112 16298 0.10% 113 16573 0.11% 114 17557 0.11% 115 18452 0.12% 116 18999 0.12% 117 19857 0.13% 118 20601 0.13% 119 20767 0.13% 120 21808 0.14% 121 22323 0.14% 122 23298 0.15% 123 23981 0.15% 124 24707 0.16% 125 25258 0.16% 126 26706 0.17% 127 26726 0.17% 128 27505 0.17% 129 28854 0.18% 130 28946 0.18% 131 29657 0.19% 132 30373 0.19% 133 31205 0.20% 134 31644 0.20% 135 32542 0.21% 136 33946 0.22% 137 33943 0.22% 138 34918 0.22% 139 36324 0.23% 140 37141 0.24% 141 37205 0.24% 142 37941 0.24% 143 39165 0.25% 144 40368 0.26% 145 41013 0.26% 146 41120 0.26% 147 42467 0.27% 148 43161 0.27% 149 44046 0.28% 150 44411 0.28% 151 14294740 90.94% 15719525 reads passed initial QC criterion=sequence-density sequence-density=0.37 sequence-density-rank=1 fanout-score=1.96 fanout-score-rank=29 prefix-density=0.38 prefix-fanout=1.9 sequence=CGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTTCGAGCGAAGAAGGTAATCAATTTCCTTGGCCAATTCCTCCTCTGTGAGATCTGGAAGGTAAGAAAGAGTCTCGAACTTCTTCAATCCAGTTGGTGGCCACACCTGCATGCATTGAACTC criterion=fanout-score sequence-density=0.10 sequence-density-rank=23 fanout-score=8.83 fanout-score-rank=1 prefix-density=0.18 prefix-fanout=4.9 sequence=GCAGCTTCCATCATCACTCCT criterion=sequence-density sequence-density=0.54 sequence-density-rank=1 fanout-score=2.21 fanout-score-rank=26 prefix-density=0.56 prefix-fanout=2.1 sequence=CCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAATCATCATGTCTAGCACCTGCGACACCTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAGTGAACAAACAAAAGTGTCCGTGAGTGTCTTTATCTTTGTGTCTT criterion=fanout-score sequence-density=0.13 sequence-density-rank=26 fanout-score=34.06 fanout-score-rank=1 prefix-density=0.37 prefix-fanout=12.0 sequence=AAAGAAAAGAAAA SRR13695471 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 06:04:09 Started mapping on | Feb 12 06:04:10 Finished on | Feb 12 06:05:34 Mapping speed, Million of reads per hour | 673.69 Number of input reads | 15719525 Average input read length | 289 UNIQUE READS: Uniquely mapped reads number | 13740020 Uniquely mapped reads % | 87.41% Average mapped length | 289.59 Number of splices: Total | 13621367 Number of splices: Annotated (sjdb) | 13328999 Number of splices: GT/AG | 13348691 Number of splices: GC/AG | 216905 Number of splices: AT/AC | 7066 Number of splices: Non-canonical | 48705 Mismatch rate per base, % | 0.40% Deletion rate per base | 0.03% Deletion average length | 2.88 Insertion rate per base | 0.02% Insertion average length | 2.45 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 346252 % of reads mapped to multiple loci | 2.20% Number of reads mapped to too many loci | 105700 % of reads mapped to too many loci | 0.67% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 9.59% % of reads unmapped: other | 0.13% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 1633423 1633423 1633423 N_multimapping 346252 346252 346252 N_noFeature 599477 13438300 777661 N_ambiguous 250202 3346 124016 UnstrandedReadsAssigned:12890341 PositiveStrandReadsAssigned:298374 NegativeStrandReadsAssigned:12838343 Dataset is classified negative stranded MeadianReadLen=143 20thPercentileLength=143 echo kmer=139 SRR13695471 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695471-trimmed-pair1.fastq SRR13695471-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 15,719,525 reads, 13,807,588 reads pseudoaligned [quant] estimated average fragment length: 253.078 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,121 rounds 52401 SRR13695471.ke.tsv 34699 SRR13695471.se.tsv 87100 total ==> SRR13695471.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1765.92 497 19.426 Potri.005G024800.1.v4.1 1035 782.922 383 33.7659 Potri.004G059700.1.v4.1 961 709.13 6 0.584015 Potri.007G009000.2.v4.1 1416 1163.92 0 0 Potri.003G141000.2.v4.1 2943 2690.92 831.513 21.3288 Potri.016G087400.1.v4.1 270 85.9082 650 522.249 Potri.015G069301.1.v4.1 564 323.355 0 0 Potri.010G195200.1.v4.1 1773 1520.92 119 5.40056 Potri.012G127500.1.v4.1 977 725.037 126 11.9952 ==> SRR13695471.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 132 Potri.001G233950.v4.1 1 Potri.001G122700.v4.1 203 Potri.001G212900.v4.1 1 Potri.001G182400.v4.1 1 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 6 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 8 SRR13695471 completed mapping pipeline successfully