Starting /dee2/code/volunteer_pipeline.sh SRR13695472 current disk space = 3048984489984 free memory = 1110861960 SRR13695472 SRAfilesize ce34e9bdad8631640c3d89f8ecc76180 SRR13695472.sra SRR13695472.sra file validated SRR13695472 is paired end SRR13695472 is conventional basespace SRR13695472 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695472_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.556 37.0 37.0 37.0 37.0 37.0 2 36.19475 37.0 37.0 37.0 37.0 37.0 3 36.523 37.0 37.0 37.0 37.0 37.0 4 36.5545 37.0 37.0 37.0 37.0 37.0 5 36.559 37.0 37.0 37.0 37.0 37.0 6 36.5675 37.0 37.0 37.0 37.0 37.0 7 36.505 37.0 37.0 37.0 37.0 37.0 8 36.5585 37.0 37.0 37.0 37.0 37.0 9 36.565 37.0 37.0 37.0 37.0 37.0 10-14 36.567499999999995 37.0 37.0 37.0 37.0 37.0 15-19 36.4808 37.0 37.0 37.0 37.0 37.0 20-24 36.538599999999995 37.0 37.0 37.0 37.0 37.0 25-29 36.466300000000004 37.0 37.0 37.0 37.0 37.0 30-34 36.4563 37.0 37.0 37.0 37.0 37.0 35-39 36.4161 37.0 37.0 37.0 37.0 37.0 40-44 36.3634 37.0 37.0 37.0 37.0 37.0 45-49 36.2995 37.0 37.0 37.0 37.0 37.0 50-54 36.3241 37.0 37.0 37.0 37.0 37.0 55-59 36.3676 37.0 37.0 37.0 37.0 37.0 60-64 36.285000000000004 37.0 37.0 37.0 37.0 37.0 65-69 36.2746 37.0 37.0 37.0 37.0 37.0 70-74 36.286 37.0 37.0 37.0 37.0 37.0 75-79 36.2659 37.0 37.0 37.0 37.0 37.0 80-84 36.2067 37.0 37.0 37.0 37.0 37.0 85-89 36.1814 37.0 37.0 37.0 37.0 37.0 90-94 36.15689999999999 37.0 37.0 37.0 37.0 37.0 95-99 36.1439 37.0 37.0 37.0 37.0 37.0 100-104 36.1456 37.0 37.0 37.0 37.0 37.0 105-109 36.1505 37.0 37.0 37.0 37.0 37.0 110-114 36.032700000000006 37.0 37.0 37.0 37.0 37.0 115-119 36.0394 37.0 37.0 37.0 37.0 37.0 120-124 35.9601 37.0 37.0 37.0 37.0 37.0 125-129 35.8301 37.0 37.0 37.0 37.0 37.0 130-134 35.769600000000004 37.0 37.0 37.0 37.0 37.0 135-139 35.7427 37.0 37.0 37.0 37.0 37.0 140-144 35.601000000000006 37.0 37.0 37.0 37.0 37.0 145-149 35.4166 37.0 37.0 37.0 37.0 37.0 150-151 35.208749999999995 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 18 1.0 19 0.0 20 0.0 21 1.0 22 0.0 23 0.0 24 1.0 25 1.0 26 7.0 27 11.0 28 12.0 29 21.0 30 37.0 31 39.0 32 57.0 33 73.0 34 144.0 35 330.0 36 2948.0 37 317.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.525 11.275 8.325000000000001 35.875 2 21.186227695400856 13.043478260869565 34.70721286755466 31.06308117617492 3 19.900000000000002 16.525000000000002 26.650000000000002 36.925000000000004 4 22.2 24.7 23.05 30.049999999999997 5 21.9 32.4 24.099999999999998 21.6 6 19.825 35.9 23.075000000000003 21.2 7 16.675 26.575 39.925 16.825000000000003 8 17.05 26.325 34.35 22.275 9 17.825 26.5 33.125 22.55 10-14 19.99 29.404999999999998 27.655 22.95 15-19 20.59 28.499999999999996 27.425 23.485 20-24 19.705000000000002 28.84 28.134999999999998 23.32 25-29 20.525 28.775000000000002 27.694999999999997 23.005 30-34 20.865000000000002 28.994999999999997 26.865 23.275000000000002 35-39 20.125 28.389999999999997 27.975 23.51 40-44 19.955000000000002 29.485 27.115000000000002 23.445 45-49 19.885 28.744999999999997 27.815 23.555 50-54 19.564999999999998 29.15 27.075 24.21 55-59 20.580000000000002 28.49 27.800000000000004 23.13 60-64 20.455000000000002 28.910000000000004 27.125 23.51 65-69 20.630000000000003 28.610000000000003 26.834999999999997 23.925 70-74 20.61 28.449999999999996 27.29 23.65 75-79 20.9 27.810000000000002 28.08 23.21 80-84 20.955 27.075 27.93 24.04 85-89 20.385 28.64 26.965 24.01 90-94 21.17 28.349999999999998 27.61 22.869999999999997 95-99 20.925 28.205000000000002 27.744999999999997 23.125 100-104 20.435 28.88 27.065 23.62 105-109 20.95 27.92 27.889999999999997 23.24 110-114 21.185000000000002 27.305 27.525 23.985 115-119 20.7 28.365000000000002 27.389999999999997 23.544999999999998 120-124 20.77 28.355000000000004 26.974999999999998 23.9 125-129 20.745 27.96 26.919999999999998 24.375 130-134 20.86 28.084999999999997 26.875 24.18 135-139 21.035 27.3 27.105 24.560000000000002 140-144 21.560000000000002 28.12 26.775 23.544999999999998 145-149 21.32 28.389999999999997 26.450000000000003 23.84 150-151 21.4 27.5875 27.150000000000002 23.8625 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.5 22 0.5 23 2.0 24 2.0 25 4.5 26 6.0 27 7.5 28 12.5 29 14.0 30 14.0 31 26.0 32 35.0 33 33.0 34 58.5 35 86.5 36 89.5 37 106.5 38 114.0 39 142.0 40 174.5 41 191.5 42 246.5 43 259.0 44 243.0 45 266.0 46 291.0 47 262.5 48 224.0 49 205.0 50 183.5 51 156.0 52 126.5 53 93.0 54 67.5 55 61.5 56 51.5 57 46.5 58 33.5 59 17.0 60 14.5 61 14.0 62 9.0 63 3.5 64 1.5 65 0.5 66 0.5 67 0.5 68 0.5 69 0.5 70 0.0 71 0.5 72 0.5 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.525 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.0 #Duplication Level Percentage of deduplicated Percentage of total 1 72.17857142857143 50.525 2 17.892857142857142 25.05 3 6.678571428571428 14.025000000000002 4 1.9285714285714284 5.4 5 0.9285714285714286 3.25 6 0.3214285714285714 1.35 7 0.0 0.0 8 0.07142857142857142 0.4 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCACCAAATAGGGTATCTTCCTCTGAAAGAGTGATGTTATGAGTTTCCTT 8 0.2 No Hit CAGCATTTTTTTAATAAGTTCAACCGATCCTTAGCCTGTAGTCTTTTGAA 8 0.2 No Hit CTCCAGATATTTCCTCAGGTCACTTTCATCCACCATCCTCTTGCCATTTC 6 0.15 No Hit CCTACATGTACAACAGCAAAACAAAAGTTGACAACATAATCAAGTAAATA 6 0.15 No Hit GCTCCTTGCCGTTGAGACGCAGTTGACACAGAAGTAGGTGAAAATGGATC 6 0.15 No Hit GGGAAGGTTCTAAGCATGAATGTAGAAGAACATTCTGGAAGAAGATATTA 6 0.15 No Hit GCCAGAACCATACACCAATCCTTTCTACTTCCAGACTTGTATCTCCAAGA 6 0.15 No Hit GCATTCAAAAACATCACTCTTGAGCAAATATATTTAACAGCATGCATTTT 6 0.15 No Hit GAGAGAAGAGAAAATGGGAAAATGCTTCTGGAACTTGTGGACGGCACTAG 6 0.15 No Hit CAGGATTCTAAACTGGAAATTTAAGACCTAAAACAAGTAAATTTGGGGAG 6 0.15 No Hit CCTGCGACCAAAAGGAGGCTCCAAGTCCTAACACCAACCTACGAGGTGCT 6 0.15 No Hit CAGCAAATAACATTACAAAGTAACCCACAAAGATGTCCACAAACCAGCAC 5 0.125 No Hit GCGGTGTGAAAGAAGGACCAGTCATGAGACTAGGGATGACAGTAATGAGA 5 0.125 No Hit TGCCTTTATGTCTCTGTGTACAATCTTCAGCCTTGACTCTTCATGAAGAT 5 0.125 No Hit CGGCATTGCAAAGGTTTGCAAGAGATCGCATGTGTTTCATCCCATACTGC 5 0.125 No Hit ACCTGGTTCACAAATCCCTCGAGAGCTTCTAACTTCTCCATGTCATCAGC 5 0.125 No Hit CCCAAATTCAGCATCAAACGAAAGAAAATCATACAGATCAAGCTCTTGAC 5 0.125 No Hit GTCACATATTTGCTGTGGTTTCCCTGATTTCTTCCAATGAGATTGTCTGA 5 0.125 No Hit GCCCAGAGTAAGATGGCAGAACTGGTGCAACTACATAAGTAGGATCTTTT 5 0.125 No Hit GTTTAATAATTGGCCCCATTTCTTTACCATGATGATATACTGTTCATGGA 5 0.125 No Hit GTAGCATTAACCATCTGGTTGTTGACGGTGTATTGGGTAGTTCCACCAAG 5 0.125 No Hit GTTGCCCTTGCTAATCAAGCAGGTTCCTTCATTAACGTTGCAGACTGGGT 5 0.125 No Hit CTGGATTAAAGAGAAAAGATGGGTATTAGCTAAAATTAGTACTGCTAATC 5 0.125 No Hit GCCCATCCCAGCATCATACATCCTGGCAAACTCACGCACTGAATCCCACA 5 0.125 No Hit GATCAAAAAGCAACGTCGCTATGAACGCTTGGCTGCCACAAGCCAGTTAT 5 0.125 No Hit CCATAGGACGCAACATTTCAGGTCTAAACATGCCAGAATTACCAATCTCC 5 0.125 No Hit GTGTAGATCATGTGGAGAGGTATCAACAGATGGCGCTCTAAGCCTTTCCC 5 0.125 No Hit CGGCGACGTTTTCTCATTTGCAGGAGCATGGATCACAGGTGCAGTTTGAT 5 0.125 No Hit CTCAGCAGGGTCACGAAGCTCATCAGGATCAGCCAATGAGTGTCCTCTAA 5 0.125 No Hit CTGGTACTCCAGTCTTTGGAAAGTCAACCGCAATTGAAAATTTACGTGCA 5 0.125 No Hit CCCATTTACTAGGACCACAAATAATTAACAAGACTACAATAAATTCAAGT 5 0.125 No Hit CCCAACCAAGATGCAAAATCACTTATATCCTTGTCAGTGAAATCCACAAA 5 0.125 No Hit GCCAGAAACAGTTGTCAAAGCACCGCCATGAGCACCATCAGGACTGGTAA 5 0.125 No Hit CCCAGGTTCACAGTAACCAGATATCCATTATCAAATTTGCCATCAATGAT 5 0.125 No Hit CCCATGTCTGTATTTAGGAAACTCAAGGAGGATTCTCTCTAAACAACCAA 5 0.125 No Hit CTCTCATTTTACTTCTTCGATTTCATCCAAGGCGTAGTTGTTTGTAGATA 5 0.125 No Hit CTGCACTCAACTTGATTCTTTTGCGGATAACATAGACAAACTGTCCTACA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0125 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.0875 0.0 0.0 0.0 0.0 70-71 0.16249999999999998 0.0 0.0 0.0 0.0 72-73 0.21250000000000002 0.0 0.0 0.0 0.0 74-75 0.2625 0.0 0.0 0.0 0.0 76-77 0.3125 0.0 0.0 0.0 0.0 78-79 0.5375000000000001 0.0 0.0 0.0 0.0 80-81 0.5625 0.0 0.0 0.0 0.0 82-83 0.725 0.0 0.0 0.0 0.0 84-85 0.7875000000000001 0.0 0.0 0.0 0.0 86-87 0.8374999999999999 0.0 0.0 0.0 0.0 88-89 0.9125 0.0 0.0 0.0 0.0 90-91 1.1 0.0 0.0 0.0 0.0 92-93 1.25 0.0 0.0 0.0 0.0 94-95 1.4 0.0 0.0 0.0 0.0 96-97 1.575 0.0 0.0 0.0 0.0 98-99 1.825 0.0 0.0 0.0 0.0 100-101 2.0375 0.0 0.0 0.0 0.0 102-103 2.3499999999999996 0.0 0.0 0.0 0.0 104-105 2.75 0.0 0.0 0.0 0.0 106-107 3.0250000000000004 0.0 0.0 0.0 0.0 108-109 3.3375 0.0 0.0 0.0 0.0 110-111 3.6625 0.0 0.0 0.0 0.0 112-113 4.1125 0.0 0.0 0.0 0.0 114-115 4.525 0.0 0.0 0.0 0.0 116-117 4.95 0.0 0.0 0.0 0.0 118-119 5.449999999999999 0.0 0.0 0.0 0.0 120-121 5.887499999999999 0.0 0.0 0.0 0.0 122-123 6.4375 0.0 0.0 0.0 0.0 124-125 6.8375 0.0 0.0 0.0 0.0 126-127 7.225 0.0 0.0 0.0 0.0 128-129 7.6625 0.0 0.0 0.0 0.0 130-131 8.175 0.0 0.0 0.0 0.0 132-133 8.925 0.0 0.0 0.0 0.0 134-135 9.45 0.0 0.0 0.0 0.0 136-137 9.8875 0.0 0.0 0.0 0.0 138-139 10.8625 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCCCAT 10 0.006830828 145.0 145 >>END_MODULE SRR13695472 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695472_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.222 37.0 37.0 37.0 37.0 37.0 2 36.1965 37.0 37.0 37.0 37.0 37.0 3 36.264 37.0 37.0 37.0 37.0 37.0 4 36.216 37.0 37.0 37.0 37.0 37.0 5 36.4045 37.0 37.0 37.0 37.0 37.0 6 36.3675 37.0 37.0 37.0 37.0 37.0 7 36.3355 37.0 37.0 37.0 37.0 37.0 8 36.357 37.0 37.0 37.0 37.0 37.0 9 36.3105 37.0 37.0 37.0 37.0 37.0 10-14 36.3637 37.0 37.0 37.0 37.0 37.0 15-19 36.3925 37.0 37.0 37.0 37.0 37.0 20-24 36.397349999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.26795 37.0 37.0 37.0 37.0 37.0 30-34 36.238099999999996 37.0 37.0 37.0 37.0 37.0 35-39 36.26435 37.0 37.0 37.0 37.0 37.0 40-44 36.2665 37.0 37.0 37.0 37.0 37.0 45-49 36.2444 37.0 37.0 37.0 37.0 37.0 50-54 36.147800000000004 37.0 37.0 37.0 37.0 37.0 55-59 36.163149999999995 37.0 37.0 37.0 37.0 37.0 60-64 36.12395 37.0 37.0 37.0 37.0 37.0 65-69 36.1044 37.0 37.0 37.0 37.0 37.0 70-74 36.05265 37.0 37.0 37.0 37.0 37.0 75-79 36.08505 37.0 37.0 37.0 37.0 37.0 80-84 36.0439 37.0 37.0 37.0 37.0 37.0 85-89 35.9574 37.0 37.0 37.0 37.0 37.0 90-94 35.97235 37.0 37.0 37.0 37.0 37.0 95-99 35.9427 37.0 37.0 37.0 37.0 37.0 100-104 35.90804999999999 37.0 37.0 37.0 37.0 37.0 105-109 35.9228 37.0 37.0 37.0 37.0 37.0 110-114 35.85785 37.0 37.0 37.0 37.0 37.0 115-119 35.80155 37.0 37.0 37.0 37.0 37.0 120-124 35.73245 37.0 37.0 37.0 37.0 37.0 125-129 35.66435 37.0 37.0 37.0 37.0 37.0 130-134 35.5743 37.0 37.0 37.0 37.0 37.0 135-139 35.554500000000004 37.0 37.0 37.0 37.0 37.0 140-144 35.42005 37.0 37.0 37.0 34.6 37.0 145-149 35.339999999999996 37.0 37.0 37.0 37.0 37.0 150-151 34.954375 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 1.0 13 0.0 14 2.0 15 1.0 16 0.0 17 0.0 18 1.0 19 1.0 20 0.0 21 2.0 22 3.0 23 2.0 24 2.0 25 1.0 26 14.0 27 9.0 28 10.0 29 10.0 30 26.0 31 41.0 32 58.0 33 92.0 34 193.0 35 532.0 36 2780.0 37 219.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.072252885097846 21.500250878073256 11.138986452584044 25.288509784244855 2 28.7 26.525 28.525 16.25 3 21.75 28.125 31.424999999999997 18.7 4 24.05 33.6 23.325000000000003 19.025 5 25.7 34.575 22.375 17.349999999999998 6 20.375 38.7 22.975 17.95 7 18.35 22.825 38.875 19.950000000000003 8 21.025 25.45 29.849999999999998 23.674999999999997 9 22.1 25.174999999999997 30.175 22.55 10-14 23.805 29.34 26.150000000000002 20.705000000000002 15-19 23.544999999999998 28.04 27.229999999999997 21.185000000000002 20-24 22.64613230661533 29.056452822641134 26.911345567278367 21.386069303465174 25-29 23.475868967241812 28.432108027006752 27.446861715428856 20.64516129032258 30-34 22.55225522552255 28.24282428242824 27.927792779277926 21.277127712771275 35-39 23.651182559127957 27.641382069103454 27.796389819490976 20.911045552277614 40-44 23.17231723172317 28.487848784878487 27.247724772477248 21.09210921092109 45-49 22.972297229722972 28.657865786578657 27.94279427942794 20.427042704270427 50-54 23.255 27.750000000000004 27.455000000000002 21.54 55-59 23.20580145036259 27.771942985746435 28.02200550137534 21.00025006251563 60-64 23.101155057752887 27.85139256962848 28.16140807040352 20.88604430221511 65-69 22.89 27.525 27.655 21.93 70-74 23.59089772443111 27.076769192298073 27.831957989497376 21.500375093773442 75-79 23.06345951892784 28.004200630094516 27.459118867830174 21.473220983147474 80-84 23.305 27.825 27.275 21.595 85-89 23.94478895779156 27.50550110022004 27.740548109621926 20.809161832366474 90-94 23.221161058052903 27.58137906895345 28.066403320166007 21.13105655282764 95-99 22.835 27.21 28.185 21.77 100-104 23.905976494123532 27.911977994498628 27.741935483870968 20.44011002750688 105-109 23.76475295059012 27.670534106821364 26.9003800760152 21.664332866573314 110-114 24.381219060953047 28.536426821341067 26.95134756737837 20.131006550327516 115-119 24.313647047057056 27.86918037705656 27.094064109616443 20.72310846626994 120-124 24.491122780695175 27.881970492623154 27.56689172293073 20.060015003750937 125-129 24.708706305945892 27.729159373906086 27.219082862429367 20.34305145771866 130-134 25.42517006802721 27.74609843937575 26.87575030012005 19.95298119247699 135-139 25.58267480244073 27.21816544963489 27.123136941082326 20.076022806842055 140-144 25.641282064103205 27.13635681784089 27.791389569478476 19.43097154857743 145-149 25.48264479343803 28.663599079723916 26.61298389516855 19.2407722316695 150-151 27.07942464040025 26.75422138836773 26.6541588492808 19.51219512195122 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.0 12 1.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 1.0 22 1.0 23 3.0 24 3.5 25 2.5 26 2.0 27 3.5 28 6.0 29 9.0 30 11.5 31 18.0 32 24.0 33 35.0 34 49.0 35 66.5 36 79.5 37 97.0 38 139.5 39 172.0 40 172.5 41 207.0 42 258.5 43 286.5 44 277.0 45 239.5 46 244.0 47 258.0 48 253.5 49 212.5 50 167.5 51 151.5 52 122.5 53 99.5 54 91.0 55 69.5 56 46.5 57 30.0 58 22.0 59 15.0 60 14.5 61 15.0 62 8.0 63 3.5 64 2.0 65 0.5 66 0.0 67 1.0 68 1.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.35000000000000003 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.005 25-29 0.025 30-34 0.01 35-39 0.005 40-44 0.01 45-49 0.01 50-54 0.0 55-59 0.025 60-64 0.005 65-69 0.0 70-74 0.025 75-79 0.015 80-84 0.0 85-89 0.02 90-94 0.005 95-99 0.0 100-104 0.025 105-109 0.02 110-114 0.005 115-119 0.015 120-124 0.025 125-129 0.015 130-134 0.04 135-139 0.03 140-144 0.005 145-149 0.03 150-151 0.0625 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.89999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 72.38912732474965 50.6 2 17.989985693848354 25.15 3 6.115879828326181 12.825000000000001 4 2.03862660944206 5.7 5 0.8583690987124464 3.0 6 0.4291845493562232 1.7999999999999998 7 0.1072961373390558 0.525 8 0.07153075822603719 0.4 9 0.0 0.0 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTTTCATCATTGGGAACGAACAAAGTCGGATTTCCTGCTGCTATTCTAAA 8 0.2 No Hit CCATATGCTATAACCAGTAAGGGTTCTTATTTGGTTTGCTCTCAACTAAT 8 0.2 No Hit AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 7 0.17500000000000002 No Hit AGACAACTCGAAAGGAGTATGCTATCCTCTTCAGATCCTGGTAGTGTCTC 7 0.17500000000000002 No Hit ATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATCAAT 7 0.17500000000000002 No Hit GTTTGTTCTGCCCTCCCACCTAAAAAAATCCCAAAAAAATTCTCCTTTTC 6 0.15 No Hit ATTTCAAAACCATAGCTACTGAGCTTCAGGTACTTATTAATCTCTTCAAC 6 0.15 No Hit CAGTGCCCTACATACCTGGGCTCGGAGAACCCCATCCACTTTGCAACTGG 6 0.15 No Hit CTGAGCTTGAGTGCATTCTTCTAAGTAAAAGAAATCCCTTAATTTCATCA 6 0.15 No Hit ACCGCTAGTGCTTCTGAAGGGAGCCAATTTTATAAGACTATTGAGTATTT 6 0.15 No Hit GAAACAGAGCCAGGGACAAGATCCTTGGTGGTAAGATTATACGAGTGGAA 6 0.15 No Hit CACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAATC 6 0.15 No Hit GAAAATGTCGAGGATGGTTAATCTTCTTGTACTTCCACATCCACAACAAA 6 0.15 No Hit GTCCAAGAAGGAGAAGCTCGACAGGAAGAGGAAACCTGTGTCGAAGGAGG 6 0.15 No Hit GGAAACCCCTTGCAACTTCAGCAGAATTATCCAGTTCCAATAGCTCCTCG 6 0.15 No Hit GCCTATTTTTGTTTGTTGAATTTGACGAACTGGGTTGTTCTTTGTTTTTC 6 0.15 No Hit CGTATTCATAGGAGGAATGGCGAAGAGTTACTTCAAGCAAGAGCATGGTC 6 0.15 No Hit CTTCAATTTCCCAACTACAATTACCAGTGCATCATTTGTCTTAAGGAAGC 5 0.125 No Hit AGATTACATCACTTGCAAAGGATTTTAAGCTTGGGAGGATGCCGAGAGAG 5 0.125 No Hit GTCGTGTTAGGTGTCCTTTATTGATAAAAATGATTCAGGTCATGATAGGA 5 0.125 No Hit CATCAACGAGTATGTCCAGAGCTGTGACATCGTGGCCTTCAACAAAATCT 5 0.125 No Hit GAGGGAGGTGGCCAAGGAGGCAATTGGAAGGGCTAGGAGAGGAGAAGGAC 5 0.125 No Hit GGATTCACTGGGTGCACCTCTTTGCCCCTGCAGGCATTATGATGACAAAG 5 0.125 No Hit CAGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 5 0.125 No Hit GAAGCAATGTCACATCGAATGCATATTGACCACAGCATAAAACTTATTGG 5 0.125 No Hit CTGGGAATCATCTTGCCAATATTATGATTTTCGAGTTCCAAATGTCGTCC 5 0.125 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 5 0.125 No Hit AGTTGATTCCTAGTCAGCAAATCTCACCAATGGACTATACCCCAGAGCCC 5 0.125 No Hit GTTGGATCTCCAAAACTACGAACCTGATCCTCGCGAAACAGTGGAGAACA 5 0.125 No Hit GGTTTATTGAAACACTGATCACTGCTCACAGATGAAGAGATGAGAGACAG 5 0.125 No Hit GTAGATCGAACTCATCTAGCAGAATTCCACCAGATAGAAGGTCTGGTGTG 5 0.125 No Hit GAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCA 5 0.125 No Hit AGAACTTCTAAATGTTCTCATGTCACAACTTGAGCCTTTGACTGATCAAC 5 0.125 No Hit CATTGCTAGTCCAACAGGAAAATGTGGAATTGCAATAGAACCCTCTTACC 5 0.125 No Hit TGATGATCTAGTTGCCAAAAGTGGATGTGAGGTGGTAGTTAATGGAAGTA 5 0.125 No Hit GTTTGCTTTGTCATGATATTTAAGGTCTTCACCTATAGCCCAAAAAGATC 5 0.125 No Hit GAGGAGTTCTTTGCCTGTTCATTCAATCATGTCCTGGTACCTTGCTCTTG 5 0.125 No Hit AATCTACCGTCTTCTGTAACCAAAACTGGAACACCTCTGCAATCTGCGAA 5 0.125 No Hit AGAAGGAGGATTTGGTCCAGTTTACAAGGGTATGTTGTCAGATGGTTCTG 5 0.125 No Hit TATATTTCTAAGGGGAACCAATTTGATGGAAGATGCTCGAGGAATGGCAT 5 0.125 No Hit CTGCCCCTCTTTTTGGACCCAAGGCCGCTTCGGCGGCCGATCCGGGCGGA 5 0.125 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0125 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.05 0.0 0.0 0.0 0.0 66-67 0.075 0.0 0.0 0.0 0.0 68-69 0.0875 0.0 0.0 0.0 0.0 70-71 0.16249999999999998 0.0 0.0 0.0 0.0 72-73 0.21250000000000002 0.0 0.0 0.0 0.0 74-75 0.2625 0.0 0.0 0.0 0.0 76-77 0.3125 0.0 0.0 0.0 0.0 78-79 0.5375000000000001 0.0 0.0 0.0 0.0 80-81 0.5625 0.0 0.0 0.0 0.0 82-83 0.725 0.0 0.0 0.0 0.0 84-85 0.775 0.0 0.0 0.0 0.0 86-87 0.8125 0.0 0.0 0.0 0.0 88-89 0.8875 0.0 0.0 0.0 0.0 90-91 1.0750000000000002 0.0 0.0 0.0 0.0 92-93 1.225 0.0 0.0 0.0 0.0 94-95 1.375 0.0 0.0 0.0 0.0 96-97 1.55 0.0 0.0 0.0 0.0 98-99 1.8 0.0 0.0 0.0 0.0 100-101 2.0 0.0 0.0 0.0 0.0 102-103 2.3 0.0 0.0 0.0 0.0 104-105 2.7 0.0 0.0 0.0 0.0 106-107 2.9749999999999996 0.0 0.0 0.0 0.0 108-109 3.2875 0.0 0.0 0.0 0.0 110-111 3.6125 0.0 0.0 0.0 0.0 112-113 4.0625 0.0 0.0 0.0 0.0 114-115 4.475 0.0 0.0 0.0 0.0 116-117 4.8625 0.0 0.0 0.0 0.0 118-119 5.35 0.0 0.0 0.0 0.0 120-121 5.7875 0.0 0.0 0.0 0.0 122-123 6.3375 0.0 0.0 0.0 0.0 124-125 6.737500000000001 0.0 0.0 0.0 0.0 126-127 7.125 0.0 0.0 0.0 0.0 128-129 7.5625 0.0 0.0 0.0 0.0 130-131 8.1 0.0 0.0 0.0 0.0 132-133 8.85 0.0 0.0 0.0 0.0 134-135 9.4 0.0 0.0 0.0 0.0 136-137 9.837499999999999 0.0 0.0 0.0 0.0 138-139 10.837499999999999 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCTTCAC 10 0.006830828 145.0 145 >>END_MODULE Read 910702 spots for SRR13695472.sra Written 910702 spots for SRR13695472.sra Read 910702 spots for SRR13695472.sra Written 910702 spots for SRR13695472.sra Read 910702 spots for SRR13695472.sra Written 910702 spots for SRR13695472.sra Read 910702 spots for SRR13695472.sra Written 910702 spots for SRR13695472.sra Read 910702 spots for SRR13695472.sra Written 910702 spots for SRR13695472.sra Read 910702 spots for SRR13695472.sra Written 910702 spots for SRR13695472.sra Read 910702 spots for SRR13695472.sra Written 910702 spots for SRR13695472.sra Read 910702 spots for SRR13695472.sra Written 910702 spots for SRR13695472.sra Read 910702 spots for SRR13695472.sra Written 910702 spots for SRR13695472.sra Read 910702 spots for SRR13695472.sra Written 910702 spots for SRR13695472.sra Read 910702 spots for SRR13695472.sra Written 910702 spots for SRR13695472.sra Read 910702 spots for SRR13695472.sra Written 910702 spots for SRR13695472.sra Read 910702 spots for SRR13695472.sra Written 910702 spots for SRR13695472.sra Read 910702 spots for SRR13695472.sra Written 910702 spots for SRR13695472.sra Read 910702 spots for SRR13695472.sra Written 910702 spots for SRR13695472.sra Read 910702 spots for SRR13695472.sra Written 910702 spots for SRR13695472.sra Read 910720 spots for SRR13695472.sra Written 910720 spots for SRR13695472.sra Read 910702 spots for SRR13695472.sra Written 910702 spots for SRR13695472.sra Read 910702 spots for SRR13695472.sra Written 910702 spots for SRR13695472.sra Read 910702 spots for SRR13695472.sra Written 910702 spots for SRR13695472.sra SRR ids: ['SRR13695472.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_hrvnduen SRR13695472.sra spots: 18214058 blocks: [[1, 910702], [910703, 1821404], [1821405, 2732106], [2732107, 3642808], [3642809, 4553510], [4553511, 5464212], [5464213, 6374914], [6374915, 7285616], [7285617, 8196318], [8196319, 9107020], [9107021, 10017722], [10017723, 10928424], [10928425, 11839126], [11839127, 12749828], [12749829, 13660530], [13660531, 14571232], [14571233, 15481934], [15481935, 16392636], [16392637, 17303338], [17303339, 18214058]] SRR13695472 file size 6168233 SRR13695472 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695472 SRR13695472_1.fastq SRR13695472_2.fastq Input file: SRR13695472_1.fastq Paired file: SRR13695472_2.fastq trimmed: SRR13695472-trimmed-pair1.fastq, SRR13695472-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 05:17:33 2025 >> started Wed Feb 12 05:17:52 2025 >> done (19.506s) 18214058 read pairs processed; of these: 128 ( 0.00%) short read pairs filtered out after trimming by size control 4024 ( 0.02%) empty read pairs filtered out after trimming by size control 18209906 (99.98%) read pairs available; of these: 2486008 (13.65%) trimmed read pairs available after processing 15723898 (86.35%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 1 0.00% 19 7 0.00% 20 3 0.00% 21 2 0.00% 22 4 0.00% 23 2 0.00% 24 4 0.00% 25 5 0.00% 26 5 0.00% 27 4 0.00% 28 1 0.00% 29 9 0.00% 30 4 0.00% 31 4 0.00% 32 2 0.00% 33 7 0.00% 34 10 0.00% 35 11 0.00% 36 5 0.00% 37 5 0.00% 38 12 0.00% 39 7 0.00% 40 21 0.00% 41 19 0.00% 42 32 0.00% 43 29 0.00% 44 15 0.00% 45 27 0.00% 46 33 0.00% 47 46 0.00% 48 41 0.00% 49 81 0.00% 50 99 0.00% 51 91 0.00% 52 146 0.00% 53 144 0.00% 54 144 0.00% 55 164 0.00% 56 198 0.00% 57 243 0.00% 58 264 0.00% 59 322 0.00% 60 390 0.00% 61 430 0.00% 62 499 0.00% 63 599 0.00% 64 669 0.00% 65 716 0.00% 66 818 0.00% 67 962 0.01% 68 1001 0.01% 69 1281 0.01% 70 1573 0.01% 71 1707 0.01% 72 1958 0.01% 73 2388 0.01% 74 2594 0.01% 75 2884 0.02% 76 3267 0.02% 77 3440 0.02% 78 3843 0.02% 79 4397 0.02% 80 4719 0.03% 81 5532 0.03% 82 5872 0.03% 83 6613 0.04% 84 7583 0.04% 85 8317 0.05% 86 8580 0.05% 87 9159 0.05% 88 9944 0.05% 89 10625 0.06% 90 11166 0.06% 91 12194 0.07% 92 13152 0.07% 93 14308 0.08% 94 14847 0.08% 95 16016 0.09% 96 16811 0.09% 97 17916 0.10% 98 18697 0.10% 99 19230 0.11% 100 20253 0.11% 101 20833 0.11% 102 21987 0.12% 103 22994 0.13% 104 24104 0.13% 105 25092 0.14% 106 26137 0.14% 107 26866 0.15% 108 27428 0.15% 109 28425 0.16% 110 29206 0.16% 111 30557 0.17% 112 31457 0.17% 113 31773 0.17% 114 32941 0.18% 115 34417 0.19% 116 35430 0.19% 117 36950 0.20% 118 38141 0.21% 119 37845 0.21% 120 39987 0.22% 121 40096 0.22% 122 40730 0.22% 123 41813 0.23% 124 43102 0.24% 125 43901 0.24% 126 45857 0.25% 127 46812 0.26% 128 47127 0.26% 129 47934 0.26% 130 48202 0.26% 131 48901 0.27% 132 49671 0.27% 133 51507 0.28% 134 51933 0.29% 135 52628 0.29% 136 53762 0.30% 137 54466 0.30% 138 55547 0.31% 139 57309 0.31% 140 57631 0.32% 141 58706 0.32% 142 58904 0.32% 143 58786 0.32% 144 60838 0.33% 145 60860 0.33% 146 61550 0.34% 147 62909 0.35% 148 63810 0.35% 149 64418 0.35% 150 64505 0.35% 151 15723898 86.35% 18209906 reads passed initial QC criterion=sequence-density sequence-density=0.51 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=23 prefix-density=0.52 prefix-fanout=2.0 sequence=CGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTTCGAGCGAAGAAGGT criterion=fanout-score sequence-density=0.02 sequence-density-rank=25 fanout-score=299.29 fanout-score-rank=1 prefix-density=0.31 prefix-fanout=16.0 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGTTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGAGTATGAATGTGTTCTCTGTG criterion=sequence-density sequence-density=0.99 sequence-density-rank=1 fanout-score=1.96 fanout-score-rank=28 prefix-density=0.99 prefix-fanout=2.0 sequence=CTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAGTGAACAAACAAAAGTGTCCGTGAGTGTCTTTATCTTTGTGTCTTCTTCA criterion=fanout-score sequence-density=0.13 sequence-density-rank=23 fanout-score=31.78 fanout-score-rank=1 prefix-density=0.36 prefix-fanout=11.4 sequence=AAAGAAAAGAAAA SRR13695472 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 05:18:29 Started mapping on | Feb 12 05:18:29 Finished on | Feb 12 05:20:08 Mapping speed, Million of reads per hour | 662.18 Number of input reads | 18209906 Average input read length | 294 UNIQUE READS: Uniquely mapped reads number | 17101527 Uniquely mapped reads % | 93.91% Average mapped length | 293.47 Number of splices: Total | 16893353 Number of splices: Annotated (sjdb) | 16553355 Number of splices: GT/AG | 16540256 Number of splices: GC/AG | 286577 Number of splices: AT/AC | 9255 Number of splices: Non-canonical | 57265 Mismatch rate per base, % | 0.37% Deletion rate per base | 0.03% Deletion average length | 2.79 Insertion rate per base | 0.02% Insertion average length | 2.32 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 384967 % of reads mapped to multiple loci | 2.11% Number of reads mapped to too many loci | 111864 % of reads mapped to too many loci | 0.61% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.21% % of reads unmapped: other | 0.15% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 723633 723633 723633 N_multimapping 384967 384967 384967 N_noFeature 646424 16649088 913122 N_ambiguous 289052 1875 102111 UnstrandedReadsAssigned:16166051 PositiveStrandReadsAssigned:450564 NegativeStrandReadsAssigned:16086294 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695472 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695472-trimmed-pair1.fastq SRR13695472-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 18,209,906 reads, 16,143,435 reads pseudoaligned [quant] estimated average fragment length: 240.558 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,086 rounds 52401 SRR13695472.ke.tsv 34699 SRR13695472.se.tsv 87100 total ==> SRR13695472.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1778.44 427 12.8073 Potri.005G024800.1.v4.1 1035 795.442 273 18.3072 Potri.004G059700.1.v4.1 961 721.519 5 0.36965 Potri.007G009000.2.v4.1 1416 1176.44 0 0 Potri.003G141000.2.v4.1 2943 2703.44 991.082 19.5552 Potri.016G087400.1.v4.1 270 88.6902 735 442.059 Potri.015G069301.1.v4.1 564 332.679 0 0 Potri.010G195200.1.v4.1 1773 1533.44 93 3.23507 Potri.012G127500.1.v4.1 977 737.47 155 11.2113 ==> SRR13695472.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 197 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 302 Potri.001G212900.v4.1 8 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 3 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 10 SRR13695472 completed mapping pipeline successfully