Starting /dee2/code/volunteer_pipeline.sh SRR13695473 current disk space = 3049932763136 free memory = 1299368228 SRR13695473 SRAfilesize a76b2725d8a0c900a58b0b4d2e258309 SRR13695473.sra SRR13695473.sra file validated SRR13695473 is paired end SRR13695473 is conventional basespace SRR13695473 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695473_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.51 37.0 37.0 37.0 37.0 37.0 2 36.298 37.0 37.0 37.0 37.0 37.0 3 36.544 37.0 37.0 37.0 37.0 37.0 4 36.574 37.0 37.0 37.0 37.0 37.0 5 36.611 37.0 37.0 37.0 37.0 37.0 6 36.569 37.0 37.0 37.0 37.0 37.0 7 36.485 37.0 37.0 37.0 37.0 37.0 8 36.5905 37.0 37.0 37.0 37.0 37.0 9 36.538 37.0 37.0 37.0 37.0 37.0 10-14 36.579299999999996 37.0 37.0 37.0 37.0 37.0 15-19 36.5308 37.0 37.0 37.0 37.0 37.0 20-24 36.5373 37.0 37.0 37.0 37.0 37.0 25-29 36.469100000000005 37.0 37.0 37.0 37.0 37.0 30-34 36.441 37.0 37.0 37.0 37.0 37.0 35-39 36.383900000000004 37.0 37.0 37.0 37.0 37.0 40-44 36.406 37.0 37.0 37.0 37.0 37.0 45-49 36.404700000000005 37.0 37.0 37.0 37.0 37.0 50-54 36.3492 37.0 37.0 37.0 37.0 37.0 55-59 36.34160000000001 37.0 37.0 37.0 37.0 37.0 60-64 36.3467 37.0 37.0 37.0 37.0 37.0 65-69 36.305899999999994 37.0 37.0 37.0 37.0 37.0 70-74 36.3355 37.0 37.0 37.0 37.0 37.0 75-79 36.2268 37.0 37.0 37.0 37.0 37.0 80-84 36.2204 37.0 37.0 37.0 37.0 37.0 85-89 36.244099999999996 37.0 37.0 37.0 37.0 37.0 90-94 36.1425 37.0 37.0 37.0 37.0 37.0 95-99 36.09310000000001 37.0 37.0 37.0 37.0 37.0 100-104 36.171899999999994 37.0 37.0 37.0 37.0 37.0 105-109 36.1229 37.0 37.0 37.0 37.0 37.0 110-114 36.099999999999994 37.0 37.0 37.0 37.0 37.0 115-119 36.1248 37.0 37.0 37.0 37.0 37.0 120-124 35.9747 37.0 37.0 37.0 37.0 37.0 125-129 35.92 37.0 37.0 37.0 37.0 37.0 130-134 35.967200000000005 37.0 37.0 37.0 37.0 37.0 135-139 35.881299999999996 37.0 37.0 37.0 37.0 37.0 140-144 35.7955 37.0 37.0 37.0 37.0 37.0 145-149 35.7327 37.0 37.0 37.0 37.0 37.0 150-151 35.526 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 25 3.0 26 6.0 27 6.0 28 14.0 29 20.0 30 31.0 31 36.0 32 62.0 33 72.0 34 113.0 35 318.0 36 2943.0 37 376.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.95 10.15 12.375 47.525 2 20.49221496735309 14.716223003515822 36.26318432948267 28.528377699648416 3 18.85 17.275 23.9 39.975 4 23.0 25.1 23.275000000000002 28.625 5 23.799999999999997 32.824999999999996 24.349999999999998 19.025 6 21.125 36.65 23.65 18.575 7 15.525 27.825 40.725 15.925 8 17.125 25.2 33.0 24.675 9 17.299999999999997 23.95 34.050000000000004 24.7 10-14 19.695 30.049999999999997 27.615000000000002 22.64 15-19 20.155 28.28 27.875 23.69 20-24 19.5 28.345 28.825 23.330000000000002 25-29 20.044999999999998 28.505000000000003 28.110000000000003 23.34 30-34 19.97 29.315 27.12 23.595 35-39 19.925 27.584999999999997 28.134999999999998 24.355 40-44 20.380000000000003 28.025 27.905 23.69 45-49 19.435 28.73 27.57 24.265 50-54 20.205000000000002 28.110000000000003 28.244999999999997 23.44 55-59 20.015 28.775000000000002 27.755000000000003 23.455000000000002 60-64 19.96 28.17 27.3 24.57 65-69 20.61 28.910000000000004 27.200000000000003 23.28 70-74 20.25 28.799999999999997 27.365000000000002 23.585 75-79 20.345 27.93 28.134999999999998 23.59 80-84 20.055 28.4 28.015 23.53 85-89 20.995 27.625 27.965 23.415 90-94 19.875 28.660000000000004 28.22 23.244999999999997 95-99 20.405 27.965 27.83 23.799999999999997 100-104 21.185000000000002 28.765 26.484999999999996 23.565 105-109 19.835 28.26 28.21 23.695 110-114 20.815 28.335 27.35 23.5 115-119 20.505000000000003 28.345 28.205000000000002 22.945 120-124 20.945 27.32 27.98 23.755000000000003 125-129 21.05 28.705000000000002 27.55 22.695 130-134 20.724999999999998 28.075 27.939999999999998 23.26 135-139 21.345 27.884999999999998 27.060000000000002 23.71 140-144 20.849999999999998 27.884999999999998 27.785 23.48 145-149 20.955 27.905 27.92 23.22 150-151 21.8125 28.125 26.437500000000004 23.625 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.5 14 0.5 15 0.5 16 0.5 17 0.0 18 0.0 19 0.0 20 0.5 21 2.0 22 4.5 23 4.0 24 2.0 25 2.5 26 9.0 27 12.5 28 9.0 29 16.5 30 19.5 31 18.5 32 35.0 33 50.5 34 57.5 35 73.0 36 95.0 37 101.0 38 111.0 39 147.5 40 180.5 41 218.0 42 258.0 43 255.0 44 224.5 45 240.0 46 278.0 47 259.0 48 227.5 49 212.5 50 176.5 51 164.0 52 139.5 53 102.0 54 76.5 55 50.0 56 49.5 57 41.0 58 28.5 59 19.5 60 12.5 61 6.5 62 2.5 63 2.0 64 1.0 65 0.5 66 0.5 67 0.5 68 0.0 69 0.5 70 0.5 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.44999999999999996 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.39999999999999 #Duplication Level Percentage of deduplicated Percentage of total 1 72.55043227665706 50.349999999999994 2 16.894812680115272 23.45 3 6.9164265129683 14.399999999999999 4 2.1253602305475505 5.8999999999999995 5 0.9726224783861672 3.375 6 0.2881844380403458 1.2 7 0.18011527377521613 0.8750000000000001 8 0.03602305475504323 0.2 9 0.0 0.0 >10 0.03602305475504323 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source TGCCAAGTTTCACCAGGTGCATCATCACCAGTGATCTTAGTGTTCAAAAG 10 0.25 No Hit TTTCTGACGATTTTCCCATCTGCTCTATTCAGTTCCCAGCAGCCAAGAAA 8 0.2 No Hit TGCAGGAGAAATTATCCAGGAAAAGTCACAATCTCTATTGTTCAACATCG 7 0.17500000000000002 No Hit ATTAGATGCTGCAACCACTGAATCGACCAAGGTTAGTCCTGCTCCAGCTG 7 0.17500000000000002 No Hit CGCTAAACTCTTCCTCTTTGTGTGTCTCGATGACAACATCAGACGTAGGA 7 0.17500000000000002 No Hit CTCCACAGCAATGGCACCAAGAGTACCCAACATTGCCCACCTTCCATGAA 7 0.17500000000000002 No Hit CTCTTTCTTACGCAACTCCTCGATAGCTCTCTCAAGAGAACCACATTCCT 7 0.17500000000000002 No Hit CATCTCCTCAAACTGAGCTTCCAAATTAGGAAACTCCTCCTCCACACCAA 6 0.15 No Hit CCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCAGAACC 6 0.15 No Hit ATGGAAAGGCCAGATGAAAGCAAGCCACCCAAGTAAAGATATTCTCTACG 6 0.15 No Hit GGCAGCCACTTAATGCCATAGTAAGTTCAAACTCCTCACGCAGCATTTGA 6 0.15 No Hit GACCAAACTGCCAGCCAGCAGGCACCGCATTGTAGCTTGTGACAGTTCTA 6 0.15 No Hit GCCCAGTTGTAGTAGTTGGATTCTATGTTTGAAATGATATCCTCAACCTC 6 0.15 No Hit GGGGTATAATGATACTGATGTTGAGAAGCTTAGCCTTAATGGTGGTGCAA 6 0.15 No Hit CTCCAACTGTTTCTTCACACTCATATCAATCATCTTAGACCCTGAGCTCC 6 0.15 No Hit CCACTAACAACACCGTGCTTTCTTTTTTTGAACCTCAAAAGCTGATTCAA 5 0.125 No Hit TGGTTTTGCCATAACTATTTCTGGAAGAAAATCCTGTTTACAACATCAAC 5 0.125 No Hit GGGTAGCTTCCACATAGTCCAGTAGCGTCCATCATAGTACCCTGGTGAGC 5 0.125 No Hit GAGGAAGAGGAAGTTCAAGCTGAAGCTGAAGTTGAAGGAGCTGTTAAAGA 5 0.125 No Hit GTTCGATTAGTCTTTCGCCCCTATACCCAAGTCAGACGAACGATTTGCAC 5 0.125 No Hit GCTTCAAACAATAATTAATAGACAGAAGATTGCAGCCAGACATAAAAAAA 5 0.125 No Hit TGTACATGTAAGGAATGTTAATTGCTCCTGCTGCATGTCCAGTACTAAAC 5 0.125 No Hit GTCCAGAGTAAATTATTGCGCATGGGGCTGCTGGACACCCAGGGATTGTT 5 0.125 No Hit GGCATATCCCAGCAAGAATATAAACAACATCATAGAGGCTTCCCTTGTCG 5 0.125 No Hit GCCAAAACAATACACATCATGAGCACAAGTTGCCGGAGATGAGCCAGAAG 5 0.125 No Hit CTCCCTTATTGTCACTTGCTAATATCTGCATGTATACTTTTGTTTCCTTC 5 0.125 No Hit GAGTGGATCAGAGTCTGTGGCATCATTGTGATCTACTGCAGGGGACGTTC 5 0.125 No Hit GTGTTTCATTCCATACTGAGTCAGTGATCCACAATGTTTTTCAAACAATC 5 0.125 No Hit CTAAGATTCCCTTCATCTTTCCCTCGGACTCCTCCTTGATTGCAGCTTTA 5 0.125 No Hit GTTGGCTAAACTTCTTGTGCACATATTGATTCATTAACGACGTCTTGCCG 5 0.125 No Hit CTCAGTAGTGAGATCTGGAAGGTAAGAAAGAGTCTCGAACTTCTTCAATC 5 0.125 No Hit CGATCTTTTTGCCAGAGCCCAGGTACAATTTGAACAAAGCAACCCTAACA 5 0.125 No Hit CCCACTATCACTCTGACCCTTCACTCCAACCTCACATCCTTCAAAGAACC 5 0.125 No Hit CTAGTATCCACTCTATCAAAACTGCCACGGTATCTGTTCCTCTAAAAATC 5 0.125 No Hit CAGGTATTCAGTTTCTTCAGGGGTGAAGGAGGAAGGGGGCTTAGCCTGCG 5 0.125 No Hit CCCGTGTCAGGATTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTG 5 0.125 No Hit TGGGTGTTTAATTAAATGCAAAATCGAGTGTTTTTCTCCAGGTGCTTGCA 5 0.125 No Hit GCGTGCGTTGGATAATGAAATAACTTGGAACGAATGTTACCTGCAATAAC 5 0.125 No Hit CTCCAAAGCAATCTTCTTATCAACCACAACAGCATCCACAGTGTACCCAT 5 0.125 No Hit CTTTATTTTATTTTTATGAAGAACATGTGTGACAAATTTATGGTTGCATG 5 0.125 No Hit CTCGACATTGTAACGAACTGTCCATCCATGACTGACACCAGGGAATATTT 5 0.125 No Hit CGGTACTACACGAAACTGATATCTTTATGGAAGCAACAGAATCTACTAGA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.1 0.0 0.0 0.0 0.0 80-81 0.125 0.0 0.0 0.0 0.0 82-83 0.125 0.0 0.0 0.0 0.0 84-85 0.175 0.0 0.0 0.0 0.0 86-87 0.3125 0.0 0.0 0.0 0.0 88-89 0.3875 0.0 0.0 0.0 0.0 90-91 0.48750000000000004 0.0 0.0 0.0 0.0 92-93 0.6375 0.0 0.0 0.0 0.0 94-95 0.6875 0.0 0.0 0.0 0.0 96-97 0.8875 0.0 0.0 0.0 0.0 98-99 1.1875 0.0 0.0 0.0 0.0 100-101 1.3375 0.0 0.0 0.0 0.0 102-103 1.5625 0.0 0.0 0.0 0.0 104-105 1.7375 0.0 0.0 0.0 0.0 106-107 2.0125 0.0 0.0 0.0 0.0 108-109 2.1625 0.0 0.0 0.0 0.0 110-111 2.45 0.0 0.0 0.0 0.0 112-113 2.675 0.0 0.0 0.0 0.0 114-115 2.8625 0.0 0.0 0.0 0.0 116-117 3.1875 0.0 0.0 0.0 0.0 118-119 3.55 0.0 0.0 0.0 0.0 120-121 3.8375 0.0 0.0 0.0 0.0 122-123 4.25 0.0 0.0 0.0 0.0 124-125 4.6125 0.0 0.0 0.0 0.0 126-127 4.949999999999999 0.0 0.0 0.0 0.0 128-129 5.2875 0.0 0.0 0.0 0.0 130-131 5.6625 0.0 0.0 0.0 0.0 132-133 6.0 0.0 0.0 0.0 0.0 134-135 6.55 0.0 0.0 0.0 0.0 136-137 7.2375 0.0 0.0 0.0 0.0 138-139 7.825 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR13695473 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695473_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.28875 37.0 37.0 37.0 37.0 37.0 2 36.134 37.0 37.0 37.0 37.0 37.0 3 36.2265 37.0 37.0 37.0 37.0 37.0 4 36.232 37.0 37.0 37.0 37.0 37.0 5 36.293 37.0 37.0 37.0 37.0 37.0 6 36.2955 37.0 37.0 37.0 37.0 37.0 7 36.3525 37.0 37.0 37.0 37.0 37.0 8 36.3615 37.0 37.0 37.0 37.0 37.0 9 36.339 37.0 37.0 37.0 37.0 37.0 10-14 36.283100000000005 37.0 37.0 37.0 37.0 37.0 15-19 36.2138 37.0 37.0 37.0 37.0 37.0 20-24 36.23270000000001 37.0 37.0 37.0 37.0 37.0 25-29 36.216449999999995 37.0 37.0 37.0 37.0 37.0 30-34 36.17685 37.0 37.0 37.0 37.0 37.0 35-39 36.16065 37.0 37.0 37.0 37.0 37.0 40-44 36.14015 37.0 37.0 37.0 37.0 37.0 45-49 36.133250000000004 37.0 37.0 37.0 37.0 37.0 50-54 36.10985 37.0 37.0 37.0 37.0 37.0 55-59 36.07045 37.0 37.0 37.0 37.0 37.0 60-64 36.03015 37.0 37.0 37.0 37.0 37.0 65-69 36.004650000000005 37.0 37.0 37.0 37.0 37.0 70-74 35.98524999999999 37.0 37.0 37.0 37.0 37.0 75-79 35.91775 37.0 37.0 37.0 37.0 37.0 80-84 35.92275 37.0 37.0 37.0 37.0 37.0 85-89 35.87235 37.0 37.0 37.0 37.0 37.0 90-94 35.836149999999996 37.0 37.0 37.0 37.0 37.0 95-99 35.84895 37.0 37.0 37.0 37.0 37.0 100-104 35.750249999999994 37.0 37.0 37.0 37.0 37.0 105-109 35.78395 37.0 37.0 37.0 37.0 37.0 110-114 35.664249999999996 37.0 37.0 37.0 37.0 37.0 115-119 35.66775 37.0 37.0 37.0 37.0 37.0 120-124 35.56515 37.0 37.0 37.0 37.0 37.0 125-129 35.64815 37.0 37.0 37.0 37.0 37.0 130-134 35.482150000000004 37.0 37.0 37.0 37.0 37.0 135-139 35.392250000000004 37.0 37.0 37.0 34.6 37.0 140-144 35.29795 37.0 37.0 37.0 34.6 37.0 145-149 35.16054999999999 37.0 37.0 37.0 29.8 37.0 150-151 34.912 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 1.0 19 0.0 20 2.0 21 0.0 22 1.0 23 3.0 24 6.0 25 10.0 26 10.0 27 14.0 28 13.0 29 12.0 30 23.0 31 44.0 32 63.0 33 111.0 34 239.0 35 619.0 36 2661.0 37 167.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 31.54597845151591 18.84239538962666 16.01102480581308 33.600601353044354 2 26.775 25.424999999999997 30.45 17.349999999999998 3 21.349999999999998 26.35 30.8 21.5 4 24.9 33.324999999999996 23.0 18.775 5 24.85 35.75 22.7 16.7 6 21.625 36.625 26.1 15.65 7 19.425 21.075 41.0 18.5 8 20.9 24.925 29.799999999999997 24.375 9 20.625 25.674999999999997 32.675 21.025 10-14 22.29 29.494999999999997 27.12 21.095 15-19 22.095000000000002 28.794999999999998 27.93 21.18 20-24 22.779555911182236 28.800760152030406 28.100620124024804 20.319063812762554 25-29 22.360590147536886 28.267066766691674 28.232058014503625 21.14028507126782 30-34 22.73068267066767 28.012003000750184 27.956989247311824 21.30032508127032 35-39 22.665666416604154 28.322080520130033 27.70692673168292 21.305326331582897 40-44 22.630657664416105 28.182045511377847 28.432108027006752 20.7551887971993 45-49 22.62065516379095 28.31707926981745 27.836959239809957 21.225306326581645 50-54 22.28557139284821 28.672168042010505 28.052013003250813 20.990247561890474 55-59 23.20080020005001 27.46686671667917 28.057014253563388 21.275318829707427 60-64 22.680670167541887 28.047011752938232 27.741935483870968 21.530382595648913 65-69 23.01345201780267 27.03405510826624 28.994349152372855 20.958143721558233 70-74 22.5856464116029 28.342085521380344 28.4671167791948 20.605151287821954 75-79 21.500375093773442 28.652163040760193 28.647161790447612 21.200300075018756 80-84 22.72068017004251 28.95223805951488 27.321830457614404 21.005251312828207 85-89 22.245561390347586 28.11702925731433 28.232058014503625 21.405351337834457 90-94 23.240810202550637 27.38684671167792 28.312078019504877 21.060265066266567 95-99 22.99574893723431 27.961990497624406 27.81695423855964 21.225306326581645 100-104 23.270817704426104 27.611902975743934 28.597149287321834 20.520130032508128 105-109 23.34583645911478 27.67691922980745 27.956989247311824 21.02025506376594 110-114 23.275818954738682 28.247061765441362 27.70692673168292 20.770192548137032 115-119 23.865966491622906 28.312078019504877 27.836959239809957 19.984996249062263 120-124 23.980995248812203 28.6271567891973 27.35183795948987 20.040010002500626 125-129 24.58614653663416 27.371842960740185 27.996999249812454 20.045011252813204 130-134 23.895973993498373 28.842210552638157 27.056764191047762 20.205051262815704 135-139 24.516129032258064 27.651912978244564 28.00700175043761 19.824956239059766 140-144 25.506376594148538 28.02700675168792 26.89672418104526 19.56989247311828 145-149 25.98649662415604 27.506876719179797 26.76669167291823 19.739934983745936 150-151 27.00675168792198 27.481870467616904 26.244061015253813 19.2673168292073 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 1.5 10 1.5 11 0.0 12 0.0 13 0.5 14 2.0 15 1.5 16 0.0 17 0.5 18 0.5 19 0.0 20 0.0 21 1.0 22 3.0 23 2.5 24 6.0 25 7.0 26 3.5 27 3.5 28 5.0 29 11.5 30 24.5 31 36.0 32 42.5 33 46.0 34 60.0 35 78.5 36 98.0 37 108.5 38 125.0 39 166.0 40 204.5 41 231.5 42 252.5 43 254.0 44 244.5 45 268.5 46 271.5 47 232.5 48 234.0 49 222.0 50 155.5 51 113.5 52 95.5 53 89.5 54 82.5 55 55.5 56 39.0 57 34.0 58 21.5 59 17.5 60 17.5 61 9.0 62 7.5 63 5.5 64 0.5 65 1.5 66 1.5 67 0.5 68 0.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22499999999999998 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.02 25-29 0.025 30-34 0.025 35-39 0.025 40-44 0.025 45-49 0.025 50-54 0.025 55-59 0.025 60-64 0.025 65-69 0.015 70-74 0.025 75-79 0.025 80-84 0.025 85-89 0.025 90-94 0.025 95-99 0.025 100-104 0.025 105-109 0.025 110-114 0.025 115-119 0.025 120-124 0.025 125-129 0.025 130-134 0.025 135-139 0.025 140-144 0.025 145-149 0.025 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.125 #Duplication Level Percentage of deduplicated Percentage of total 1 73.0837789661319 51.24999999999999 2 16.8270944741533 23.599999999999998 3 6.702317290552584 14.099999999999998 4 1.9964349376114083 5.6000000000000005 5 0.8912655971479502 3.125 6 0.32085561497326204 1.35 7 0.10695187165775401 0.525 8 0.035650623885918005 0.2 9 0.0 0.0 >10 0.035650623885918005 0.25 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CTCATTGCACCAGAAAGAATCACCTTGAAAAAGGCGCCAGTTTACTACAG 10 0.25 No Hit ACCTGAGATTCTCTTCTCTTTCATGTCTATTGTGTTTGCCTAATCTTCCA 8 0.2 No Hit GACAACTTCTATTTTCACACGCCAGTTTCCTTAGACACGAACATGGCTGC 7 0.17500000000000002 No Hit GCAGAAGAGATTGACCTCCCCTACTCATGCAGGGCTGGCTCATGCTCTTC 7 0.17500000000000002 No Hit TAACCAGGAGGCACATCCCGAATCCTATCTTAGAGTTCCTGCAATCGTGA 7 0.17500000000000002 No Hit TGAAATTCTTGGATTTATGAAAGACGAACAACTGCGAAAGCATTTGCCAA 6 0.15 No Hit CATGGCTTGGCTACTTTCCATATCTCCTTATGAAGAGCAAAAGAGGGTTG 6 0.15 No Hit CGCATCTTAGGTTGTCTCTTGGGGACAATCTGGAGGAGAAAATTGCTCGT 6 0.15 No Hit CTCGACTTGGTTCCGGGATTTTTGGTTGAGTCAACATCCAGAGGAGAGTT 6 0.15 No Hit GGTGAGTTCAGTTGTGGCTTTGGAGTCACAGCATTTGGCCCAGATAGCAA 6 0.15 No Hit AGAAAGCAGCTCTTGCAAGGTTGAGTGCTGTGCACAGGAGTCTTAAGGTA 6 0.15 No Hit ATCTTGAAGAAGTTTCCAGACCATGGAGAAACTTTATCCATGAAGGGTTT 6 0.15 No Hit AGACTTGCTCCATTGACACTCTCAAGCTAGGTGCATGTGTGGATGTGTTA 6 0.15 No Hit CCGATGTCTTCAAGGCGTTGGCACTTGGAGCCTCCGGCATCTTTATTGGT 6 0.15 No Hit GCACAGGAGCTGCAAAGGCTGTGGGGAAGGTGCTACCTGCTTTGAATGGC 5 0.125 No Hit CAATGATCCAACTGTGAGGGTTGAAGAGGTTCCGGGGAGAGTTGTTGCCG 5 0.125 No Hit CCCAGTCAGTACCAGAAAGGCTAATGACATTACTTCCATTGCAAGCAATG 5 0.125 No Hit GTTCTTTCTAGCCTGATTATGATTGACAGAGTTCATTGGATTAACAAGGA 5 0.125 No Hit TAGAGGGTGTAATAGCTAAGTAGCCTGTAAGAGATGGCTTCCTCTGTGAT 5 0.125 No Hit GGAGTTTGAACTTACTATGGCATTAAGTGGCTGCCGTTCCCTCAAGGAAA 5 0.125 No Hit CTAGCAAACAAAGAAAAAAAAAGGAGCTCAGTAGCAGAATGAAGAAAGCT 5 0.125 No Hit ATTTTGAAGACGATGGTTTGCAGTCCCTGGATTGGATTACAAGACTGAAA 5 0.125 No Hit CAGAGGTTTTCAAGAAAGCTGGAACTTATGATCCAAAGCGACTTCTAGGA 5 0.125 No Hit GGTGATTGTCGCTAAATTTCCCTTCCAAGATCCTAATCTGGCAGGAGTTA 5 0.125 No Hit GAGGGAACGTGGCAGTGAAATTAGCCAGTTCTAATGATATTCAAGCTGCA 5 0.125 No Hit GAGAATTCTACTAAAACATGTTTCAGCACTTGAAACTGGCCGTCACACAT 5 0.125 No Hit CTGATGTAGCCAAAGAAATCTTGAACAGTTCAGTCTTTGCTGATCGTCCA 5 0.125 No Hit CCCTAACGTTGTCGATCTTTACTTCAACCTTGCTGCTGCTGAAGGTGTGT 5 0.125 No Hit AAAAATGGCAGCAAAGGCTGTTGTTTGCTATGGTAGCTCAACTTCTAGCA 5 0.125 No Hit GTATTTATTCTGCACTCTATTTGTCTTTTATTTCTTTGACATCGCGCAAG 5 0.125 No Hit TGCTGGTATAAATTCTTGTAGGTTTTTCCTATTGCTTTTTTCTTTTGAAT 5 0.125 No Hit TTGAATTTGACATAAGGGCTTTTGGCATTTGTGTTGTTATAGGAGGGTTA 5 0.125 No Hit CTCTTTCTCGCCCCATCTCCTTTCTTACAAACAGTGAAAATCTGGAAAAA 5 0.125 No Hit GTGCCTGTAGCTTTTAGAAGGGTGCCTTGTGTTAAGAAGGGAGGAATTAG 5 0.125 No Hit GTGTGCGAGTCAACGGGCGAGTAAACCCGTAAGGCGCAAGGAAGCTGACT 5 0.125 No Hit CTCGGATAACCGTAGTAATTCTAGAGCTAATACGTGCAACAAACCCCGAC 5 0.125 No Hit GCTGGACTTGAAATCATCTGTTCTTGTAGGCCACTCCATGTCTGGTATGA 5 0.125 No Hit ATCTGAAGAAAGAAACAATTAAGCAGCAATATCATTCGGTGAAAGAGAGG 5 0.125 No Hit TGCCATTCGAGCGGGTTTTGATGGGGTTGAGATCCATGGGGGTTATGGTT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.025 0.0 0.0 0.0 0.0 68-69 0.025 0.0 0.0 0.0 0.0 70-71 0.025 0.0 0.0 0.0 0.0 72-73 0.025 0.0 0.0 0.0 0.0 74-75 0.075 0.0 0.0 0.0 0.0 76-77 0.075 0.0 0.0 0.0 0.0 78-79 0.1 0.0 0.0 0.0 0.0 80-81 0.125 0.0 0.0 0.0 0.0 82-83 0.125 0.0 0.0 0.0 0.0 84-85 0.175 0.0 0.0 0.0 0.0 86-87 0.3125 0.0 0.0 0.0 0.0 88-89 0.3875 0.0 0.0 0.0 0.0 90-91 0.48750000000000004 0.0 0.0 0.0 0.0 92-93 0.6375 0.0 0.0 0.0 0.0 94-95 0.6875 0.0 0.0 0.0 0.0 96-97 0.8875 0.0 0.0 0.0 0.0 98-99 1.0625 0.0 0.0 0.0 0.0 100-101 1.2125 0.0 0.0 0.0 0.0 102-103 1.4375 0.0 0.0 0.0 0.0 104-105 1.6125 0.0 0.0 0.0 0.0 106-107 1.875 0.0 0.0 0.0 0.0 108-109 2.0125 0.0 0.0 0.0 0.0 110-111 2.3 0.0 0.0 0.0 0.0 112-113 2.5250000000000004 0.0 0.0 0.0 0.0 114-115 2.7125 0.0 0.0 0.0 0.0 116-117 3.0374999999999996 0.0 0.0 0.0 0.0 118-119 3.4124999999999996 0.0 0.0 0.0 0.0 120-121 3.7125 0.0 0.0 0.0 0.0 122-123 4.1625 0.0 0.0 0.0 0.0 124-125 4.5125 0.0 0.0 0.0 0.0 126-127 4.85 0.0 0.0 0.0 0.0 128-129 5.1875 0.0 0.0 0.0 0.0 130-131 5.575 0.0 0.0 0.0 0.0 132-133 5.925 0.0 0.0 0.0 0.0 134-135 6.475 0.0 0.0 0.0 0.0 136-137 7.175 0.0 0.0 0.0 0.0 138-139 7.775 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGGGGGG 100 2.0008883E-11 21.75 145 >>END_MODULE Read 1036373 spots for SRR13695473.sra Written 1036373 spots for SRR13695473.sra Read 1036373 spots for SRR13695473.sra Written 1036373 spots for SRR13695473.sra Read 1036373 spots for SRR13695473.sra Written 1036373 spots for SRR13695473.sra Read 1036373 spots for SRR13695473.sra Written 1036373 spots for SRR13695473.sra Read 1036373 spots for SRR13695473.sra Written 1036373 spots for SRR13695473.sra Read 1036373 spots for SRR13695473.sra Written 1036373 spots for SRR13695473.sra Read 1036373 spots for SRR13695473.sra Written 1036373 spots for SRR13695473.sra Read 1036373 spots for SRR13695473.sra Written 1036373 spots for SRR13695473.sra Read 1036373 spots for SRR13695473.sra Written 1036373 spots for SRR13695473.sra Read 1036373 spots for SRR13695473.sra Written 1036373 spots for SRR13695473.sra Read 1036373 spots for SRR13695473.sra Written 1036373 spots for SRR13695473.sra Read 1036373 spots for SRR13695473.sra Written 1036373 spots for SRR13695473.sra Read 1036373 spots for SRR13695473.sra Written 1036373 spots for SRR13695473.sra Read 1036373 spots for SRR13695473.sra Written 1036373 spots for SRR13695473.sra Read 1036373 spots for SRR13695473.sra Written 1036373 spots for SRR13695473.sra Read 1036373 spots for SRR13695473.sra Written 1036373 spots for SRR13695473.sra Read 1036373 spots for SRR13695473.sra Written 1036373 spots for SRR13695473.sra Read 1036390 spots for SRR13695473.sra Written 1036390 spots for SRR13695473.sra Read 1036373 spots for SRR13695473.sra Written 1036373 spots for SRR13695473.sra Read 1036373 spots for SRR13695473.sra Written 1036373 spots for SRR13695473.sra SRR ids: ['SRR13695473.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_0d8_jpzp SRR13695473.sra spots: 20727477 blocks: [[1, 1036373], [1036374, 2072746], [2072747, 3109119], [3109120, 4145492], [4145493, 5181865], [5181866, 6218238], [6218239, 7254611], [7254612, 8290984], [8290985, 9327357], [9327358, 10363730], [10363731, 11400103], [11400104, 12436476], [12436477, 13472849], [13472850, 14509222], [14509223, 15545595], [15545596, 16581968], [16581969, 17618341], [17618342, 18654714], [18654715, 19691087], [19691088, 20727477]] SRR13695473 file size 7022403 SRR13695473 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695473 SRR13695473_1.fastq SRR13695473_2.fastq Input file: SRR13695473_1.fastq Paired file: SRR13695473_2.fastq trimmed: SRR13695473-trimmed-pair1.fastq, SRR13695473-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 06:01:35 2025 >> started Wed Feb 12 06:01:59 2025 >> done (24.322s) 20727477 read pairs processed; of these: 111 ( 0.00%) short read pairs filtered out after trimming by size control 4581 ( 0.02%) empty read pairs filtered out after trimming by size control 20722785 (99.98%) read pairs available; of these: 2382888 (11.50%) trimmed read pairs available after processing 18339897 (88.50%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 2 0.00% 19 3 0.00% 20 0 0.00% 21 2 0.00% 22 1 0.00% 23 1 0.00% 24 1 0.00% 25 4 0.00% 26 2 0.00% 27 1 0.00% 28 4 0.00% 29 6 0.00% 30 3 0.00% 31 7 0.00% 32 1 0.00% 33 3 0.00% 34 8 0.00% 35 6 0.00% 36 8 0.00% 37 11 0.00% 38 22 0.00% 39 7 0.00% 40 12 0.00% 41 29 0.00% 42 34 0.00% 43 27 0.00% 44 35 0.00% 45 41 0.00% 46 42 0.00% 47 35 0.00% 48 56 0.00% 49 80 0.00% 50 99 0.00% 51 119 0.00% 52 125 0.00% 53 148 0.00% 54 151 0.00% 55 175 0.00% 56 207 0.00% 57 219 0.00% 58 294 0.00% 59 297 0.00% 60 408 0.00% 61 515 0.00% 62 536 0.00% 63 572 0.00% 64 626 0.00% 65 739 0.00% 66 831 0.00% 67 953 0.00% 68 1068 0.01% 69 1250 0.01% 70 1420 0.01% 71 1663 0.01% 72 1963 0.01% 73 2014 0.01% 74 2413 0.01% 75 2570 0.01% 76 2916 0.01% 77 3123 0.02% 78 3536 0.02% 79 3890 0.02% 80 4181 0.02% 81 4743 0.02% 82 5317 0.03% 83 6087 0.03% 84 6540 0.03% 85 7260 0.04% 86 7501 0.04% 87 8067 0.04% 88 8492 0.04% 89 9294 0.04% 90 9684 0.05% 91 10862 0.05% 92 11334 0.05% 93 12100 0.06% 94 13025 0.06% 95 13984 0.07% 96 14447 0.07% 97 15510 0.07% 98 16120 0.08% 99 16797 0.08% 100 17502 0.08% 101 17996 0.09% 102 18832 0.09% 103 20223 0.10% 104 21026 0.10% 105 21944 0.11% 106 23285 0.11% 107 24220 0.12% 108 24698 0.12% 109 25884 0.12% 110 26518 0.13% 111 27458 0.13% 112 28038 0.14% 113 29187 0.14% 114 30255 0.15% 115 32012 0.15% 116 32908 0.16% 117 33912 0.16% 118 35196 0.17% 119 35547 0.17% 120 36399 0.18% 121 37988 0.18% 122 38551 0.19% 123 39487 0.19% 124 41172 0.20% 125 41901 0.20% 126 43479 0.21% 127 44688 0.22% 128 45442 0.22% 129 45830 0.22% 130 47779 0.23% 131 47966 0.23% 132 48364 0.23% 133 50968 0.25% 134 50742 0.24% 135 52240 0.25% 136 53265 0.26% 137 55272 0.27% 138 55366 0.27% 139 57400 0.28% 140 57621 0.28% 141 58008 0.28% 142 59731 0.29% 143 59817 0.29% 144 61488 0.30% 145 62392 0.30% 146 62908 0.30% 147 63819 0.31% 148 65781 0.32% 149 66638 0.32% 150 67066 0.32% 151 18339897 88.50% 20722785 reads passed initial QC criterion=sequence-density sequence-density=0.49 sequence-density-rank=1 fanout-score=1.99 fanout-score-rank=21 prefix-density=0.49 prefix-fanout=2.0 sequence=CGGTAGACCCAACCTTTCTCCAACTCGAATTCCAAGCAAGGAACCCACTT criterion=fanout-score sequence-density=0.20 sequence-density-rank=17 fanout-score=7.89 fanout-score-rank=1 prefix-density=0.48 prefix-fanout=3.3 sequence=CCAGCAGTGTCCCAGCCGTAGTCACCAGGGAACTCACCAGTCAAGTAGGATGGGGGCTCACCAGAGAACGGGCCCAAGTATTTAACACGGTCTGGTCCGTACCATGGGCTCCCGGAGGGAACAGGCTTGGTGGTTTTCCTCATGGAGACACGGCCATTGCCCATGATCTCAGAGGAGGAGGGGTTGAGCTTCACC criterion=sequence-density sequence-density=0.66 sequence-density-rank=1 fanout-score=2.11 fanout-score-rank=20 prefix-density=0.67 prefix-fanout=2.1 sequence=TGTAAGAGATGGCTTCCTC criterion=fanout-score sequence-density=0.01 sequence-density-rank=26 fanout-score=74.55 fanout-score-rank=1 prefix-density=0.09 prefix-fanout=6.1 sequence=ACAACTTCAAGGGCAGTAGTCTTAAACCATACTCTAAAATCTTCTTATAATTCCAGTTGTAATATTCTGCTAGCATATAATGGCTTCTTCAATGAGCTTGAAGCTGGCCTGTGCCATGCTTGTAGCGATGGTTGTTAGTGCACCACTAGCAGAAGCTGCCATCTCATGTGGCCAGGTGTCAAGCAGCTTGGCACAATGTATAACCTACCTCCAGAAGGGTGGGGCTGTGCCTGCAGCTTGCTGCAGTGGGTTGAAAGGACTTAATTCTGCAGCCACGACCACCGCCGACC SRR13695473 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 06:02:38 Started mapping on | Feb 12 06:02:39 Finished on | Feb 12 06:04:45 Mapping speed, Million of reads per hour | 592.08 Number of input reads | 20722785 Average input read length | 296 UNIQUE READS: Uniquely mapped reads number | 19647190 Uniquely mapped reads % | 94.81% Average mapped length | 294.91 Number of splices: Total | 19230615 Number of splices: Annotated (sjdb) | 18830202 Number of splices: GT/AG | 18841868 Number of splices: GC/AG | 320341 Number of splices: AT/AC | 10805 Number of splices: Non-canonical | 57601 Mismatch rate per base, % | 0.39% Deletion rate per base | 0.03% Deletion average length | 2.82 Insertion rate per base | 0.02% Insertion average length | 2.44 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 515789 % of reads mapped to multiple loci | 2.49% Number of reads mapped to too many loci | 98779 % of reads mapped to too many loci | 0.48% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 2.11% % of reads unmapped: other | 0.11% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 560053 560053 560053 N_multimapping 515789 515789 515789 N_noFeature 757566 19241545 1006307 N_ambiguous 280233 1798 122108 UnstrandedReadsAssigned:18609391 PositiveStrandReadsAssigned:403847 NegativeStrandReadsAssigned:18518775 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695473 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695473-trimmed-pair1.fastq SRR13695473-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 20,722,785 reads, 18,598,517 reads pseudoaligned [quant] estimated average fragment length: 250.29 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,094 rounds 52401 SRR13695473.ke.tsv 34699 SRR13695473.se.tsv 87100 total ==> SRR13695473.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1768.71 491 14.8172 Potri.005G024800.1.v4.1 1035 785.71 327 22.214 Potri.004G059700.1.v4.1 961 711.81 14 1.0498 Potri.007G009000.2.v4.1 1416 1166.71 0 0 Potri.003G141000.2.v4.1 2943 2693.71 873.755 17.3133 Potri.016G087400.1.v4.1 270 85.5165 803 501.196 Potri.015G069301.1.v4.1 564 324.467 0 0 Potri.010G195200.1.v4.1 1773 1523.71 57 1.99671 Potri.012G127500.1.v4.1 977 727.744 143 10.4882 ==> SRR13695473.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 1076 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 325 Potri.001G212900.v4.1 0 Potri.001G182400.v4.1 1 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 59 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 28 SRR13695473 completed mapping pipeline successfully