Starting /dee2/code/volunteer_pipeline.sh SRR13695475 current disk space = 3049672015872 free memory = 1579183364 SRR13695475 SRAfilesize a97415fa2c3972fd9d3422641d403ec0 SRR13695475.sra SRR13695475.sra file validated SRR13695475 is paired end SRR13695475 is conventional basespace SRR13695475 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695475_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5275 37.0 37.0 37.0 37.0 37.0 2 36.356 37.0 37.0 37.0 37.0 37.0 3 36.5325 37.0 37.0 37.0 37.0 37.0 4 36.4965 37.0 37.0 37.0 37.0 37.0 5 36.627 37.0 37.0 37.0 37.0 37.0 6 36.6405 37.0 37.0 37.0 37.0 37.0 7 36.4485 37.0 37.0 37.0 37.0 37.0 8 36.5455 37.0 37.0 37.0 37.0 37.0 9 36.609 37.0 37.0 37.0 37.0 37.0 10-14 36.5769 37.0 37.0 37.0 37.0 37.0 15-19 36.539500000000004 37.0 37.0 37.0 37.0 37.0 20-24 36.49309999999999 37.0 37.0 37.0 37.0 37.0 25-29 36.4865 37.0 37.0 37.0 37.0 37.0 30-34 36.4581 37.0 37.0 37.0 37.0 37.0 35-39 36.4593 37.0 37.0 37.0 37.0 37.0 40-44 36.4797 37.0 37.0 37.0 37.0 37.0 45-49 36.39909999999999 37.0 37.0 37.0 37.0 37.0 50-54 36.394099999999995 37.0 37.0 37.0 37.0 37.0 55-59 36.385299999999994 37.0 37.0 37.0 37.0 37.0 60-64 36.400600000000004 37.0 37.0 37.0 37.0 37.0 65-69 36.2904 37.0 37.0 37.0 37.0 37.0 70-74 36.3252 37.0 37.0 37.0 37.0 37.0 75-79 36.3168 37.0 37.0 37.0 37.0 37.0 80-84 36.2583 37.0 37.0 37.0 37.0 37.0 85-89 36.2652 37.0 37.0 37.0 37.0 37.0 90-94 36.1724 37.0 37.0 37.0 37.0 37.0 95-99 36.1166 37.0 37.0 37.0 37.0 37.0 100-104 36.148 37.0 37.0 37.0 37.0 37.0 105-109 36.1733 37.0 37.0 37.0 37.0 37.0 110-114 36.0715 37.0 37.0 37.0 37.0 37.0 115-119 36.10380000000001 37.0 37.0 37.0 37.0 37.0 120-124 35.9901 37.0 37.0 37.0 37.0 37.0 125-129 35.9784 37.0 37.0 37.0 37.0 37.0 130-134 35.910799999999995 37.0 37.0 37.0 37.0 37.0 135-139 35.8458 37.0 37.0 37.0 37.0 37.0 140-144 35.81 37.0 37.0 37.0 37.0 37.0 145-149 35.632 37.0 37.0 37.0 37.0 37.0 150-151 35.4135 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 22 2.0 23 2.0 24 3.0 25 6.0 26 2.0 27 2.0 28 6.0 29 16.0 30 25.0 31 34.0 32 52.0 33 78.0 34 146.0 35 320.0 36 2951.0 37 355.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 38.175 11.450000000000001 6.425 43.95 2 19.744104365278474 12.719518314099348 40.2157551430005 27.32062217762168 3 17.1 19.6 25.275 38.025 4 22.55 26.700000000000003 23.0 27.750000000000004 5 22.900000000000002 32.525 24.775 19.8 6 19.875 35.099999999999994 23.425 21.6 7 13.575000000000001 29.725 40.775 15.925 8 16.0 26.85 33.625 23.525 9 18.75 23.3 34.75 23.200000000000003 10-14 19.945 30.15 27.16 22.745 15-19 20.04 28.065 28.7 23.195 20-24 20.275000000000002 28.725 28.225 22.775000000000002 25-29 19.759999999999998 28.07 28.365000000000002 23.805 30-34 19.655 28.63 28.075 23.64 35-39 20.265 27.944999999999997 27.83 23.96 40-44 19.400000000000002 28.689999999999998 28.599999999999998 23.31 45-49 19.580000000000002 27.98 28.444999999999997 23.995 50-54 19.85 29.45 27.155 23.544999999999998 55-59 20.24 27.99 27.91 23.86 60-64 19.49 28.73 27.36 24.42 65-69 20.26 28.854999999999997 28.205000000000002 22.68 70-74 20.424999999999997 28.74 26.905 23.93 75-79 18.84 28.845 28.634999999999998 23.68 80-84 20.565 27.54 27.345000000000002 24.55 85-89 19.775000000000002 28.735 27.529999999999998 23.96 90-94 20.599999999999998 28.32 28.345 22.735 95-99 20.005 29.13 26.91 23.955000000000002 100-104 20.445 28.749999999999996 27.650000000000002 23.155 105-109 20.615 28.32 27.515 23.549999999999997 110-114 20.715 28.475 27.750000000000004 23.06 115-119 21.125 28.235 27.48 23.16 120-124 20.86 27.994999999999997 28.02 23.125 125-129 20.599999999999998 29.065 26.96 23.375 130-134 21.22 28.444999999999997 27.750000000000004 22.585 135-139 20.29 28.775000000000002 27.055 23.880000000000003 140-144 20.325 28.935 26.855 23.885 145-149 20.575 28.705000000000002 26.685 24.035 150-151 20.5375 28.537499999999998 26.974999999999998 23.95 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 0.5 8 0.0 9 0.0 10 0.5 11 0.5 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 0.5 20 1.0 21 2.0 22 3.5 23 3.5 24 6.0 25 9.0 26 4.5 27 6.5 28 12.0 29 11.5 30 14.0 31 23.0 32 33.5 33 40.0 34 49.0 35 69.5 36 105.5 37 129.0 38 140.0 39 149.0 40 169.0 41 214.5 42 239.0 43 250.0 44 267.0 45 293.5 46 278.0 47 241.0 48 242.5 49 211.5 50 178.0 51 145.0 52 101.5 53 87.5 54 67.0 55 45.5 56 45.5 57 40.0 58 23.0 59 16.0 60 10.5 61 5.5 62 4.0 63 4.0 64 3.0 65 0.0 66 0.5 67 1.5 68 1.0 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.35000000000000003 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.4 #Duplication Level Percentage of deduplicated Percentage of total 1 68.5617469879518 45.525 2 19.691265060240966 26.150000000000002 3 7.454819277108434 14.85 4 2.522590361445783 6.7 5 1.0542168674698795 3.5000000000000004 6 0.338855421686747 1.35 7 0.18825301204819278 0.8750000000000001 8 0.11295180722891565 0.6 9 0.07530120481927711 0.44999999999999996 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGGGAAAGAATTCATTAATCCAAACTGGCTCACTTGTGGTTTCTCTTCCA 9 0.22499999999999998 No Hit GGACATATCAATGTTGAACCTCATAACAACTGTAGCAATCTGTGGGGCCA 9 0.22499999999999998 No Hit GTGAAATTGAAGTGATTGGTGTTCCTTGAACTGGCACTTGCATTTGTTGT 8 0.2 No Hit CCGGTCATGAGAAACTGTAATGACTGTGCCACTGTATTCTGATATGGCCT 8 0.2 No Hit ACCAGGAGTAGCCATAGGGGGTTGTTGCAGGACAGGGTATCCCATTGGTA 8 0.2 No Hit CACGAACTGCACCATCATTAAATTGAGCATTTTGCTCGTGTTTCTTACAT 7 0.17500000000000002 No Hit CAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATG 7 0.17500000000000002 No Hit CTTCACAGCTGTTGATGTGAGGGCCACTCCAGCATCGCTGTAAGTAGAGA 7 0.17500000000000002 No Hit CAGCTGCTATCACTACCTTGTTGTGAGCTATTTCCGCAATATCCTCGACA 7 0.17500000000000002 No Hit TAAACCAACCCCAATGCAGAGAGGATTAGCAGCCGGAATCCTTACAGAAT 7 0.17500000000000002 No Hit CCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCA 6 0.15 No Hit ACTGGATGTATCATCTTATGGCATGTAATCTTAACGCCCGGAGAAATTCA 6 0.15 No Hit CTAAACTAAAGCTATGAAAAAACAAAAATCATATTACAAAGGAGTTTTAT 6 0.15 No Hit CTCAATCAAAAGTTGTCAAATTAATCATAACAGAGAAAACCCATTTCAAA 6 0.15 No Hit GTGAACAATCTCAGGTTCTGGGAACCCAGCAGCCTTCGCACATGCCTTAG 6 0.15 No Hit GGCTGAAATGCTTGTATCTCTTGTTGTATCCACCAAACATCTTTGAGCTA 6 0.15 No Hit CTTACCTTTTGCATCTCAGCCTGGTAAAACAAGCCAGTCCAGGCCTGTTA 6 0.15 No Hit GCCATACAAAATTCCTTGGTCATTATCTAGTAAAAAGGCAAGTGCGTCTA 6 0.15 No Hit TCCGATTACGATGGCGGCGGCGGAATAAATATGAACTCTGTCTGCAATTG 6 0.15 No Hit GTCGATCCTAGCAACATGTTGCCTGATAAATTTAAAGACTTGAGAGAAGA 5 0.125 No Hit ATGGACTAAAGAAGGCCCTCCTGCATCACTCGCAGCAAGATGTTCCATGA 5 0.125 No Hit CAACGAACAAAGACGAGTTTGAAATGGCATTAGCATATTTACTAGATCAA 5 0.125 No Hit CTTGCAGTTTCGGTGGAGGCCCTGGAACAGTAGGGATAGCTAGGATACCA 5 0.125 No Hit GTGGGATTATTTTTCTTCAATTTTCTAGTTTTACTCTGAATTTTTTATTT 5 0.125 No Hit GGGAGGAGATTGTGGTGGTTTGGAGCTCTCCTTCGGAGTCAAAGTAGGAG 5 0.125 No Hit CTCCTCGAAGTGGTTCATCACCGACTTCACGCTTCTCATCGAACTGAATA 5 0.125 No Hit CCAACATAAAGCGAAGTCGGCACAAACTGGATCGCTCCAGGTCCCGCCGC 5 0.125 No Hit GGGTGGTTTATTTTACAAGATCTTTCCAAAACCTTTCCAAATTTTAAAGC 5 0.125 No Hit ATAAAACAAAACCAATGTATTTAGCTCTCTGTCTAAACCTTTAAACTGAC 5 0.125 No Hit CCTTTGGCTGTATCCCCATTCATTGTCATACCAGGCGACAACCTTGATCA 5 0.125 No Hit GCCAAAACCAACACCACAGCCAGCACCAAAGCCGAAAGCAGGACCCACCC 5 0.125 No Hit CGGCACACACAGAATTATTACAGTCGAAGGTTCCCTCGAATAGTTTACAT 5 0.125 No Hit AGTAACAAGTCACAGAATATCTTGCCTTACCATCTGGGCCGTCATCTCCA 5 0.125 No Hit CTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCA 5 0.125 No Hit CAGGCAGGAACGAAACACACAGACTCGTGTGTTTTGTCCTTTTAGGAGGA 5 0.125 No Hit CCACACCAGAGGCCACAGACATGGCCAATACATAACAATGAAGAAGACAC 5 0.125 No Hit TCAACTTTGTCCCTTTGGTTTCAGCCTTCATCTTCACTGCCAACTTCACC 5 0.125 No Hit GCTCCATGCATTTCTTGTAGTGCTCCTTTGTCATCCTCTCTACGGTGTCC 5 0.125 No Hit CCCTTTTCCTCCAAAATAATGGAAGTACTTCAAGTGCCTTAAAAAGCATT 5 0.125 No Hit CACAGAAGAAGCTGAGTTTTCAACCGGAAGAGAAACTACCCAGTATCTAC 5 0.125 No Hit ATAAAGACAAGGGTGTCAGCAGATGTAAGATTCAAACCAAGCCCGCCAAC 5 0.125 No Hit GCATCCAATAAGTGGGTTGGTTCAAATCACCGAATTTTGGAACCGGTTCA 5 0.125 No Hit GGCCAAGGTTGTTAGGAATAAGGTCAATTCGTGAGTCGTCTAGGCAGGTA 5 0.125 No Hit CAACAGAGTAACCGATAGACCAGCTGTAGCCTTATTTTTCCTAAAATTAC 5 0.125 No Hit CATTAGGCACTGGCTGTGACGGCACTGCTTCCTCACTACAATTGTTTCGC 5 0.125 No Hit AGAATAACTAGTTGAGTCATGAATATTTCTTCCTTGGTTATGCCATCATG 5 0.125 No Hit TCTTTGAATCCAGAAGCTTGAGTAATGGCACCAAACCACCGTTGTGTGCT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0625 0.0 0.0 0.0 0.0 84-85 0.075 0.0 0.0 0.0 0.0 86-87 0.075 0.0 0.0 0.0 0.0 88-89 0.15 0.0 0.0 0.0 0.0 90-91 0.23750000000000002 0.0 0.0 0.0 0.0 92-93 0.3 0.0 0.0 0.0 0.0 94-95 0.4 0.0 0.0 0.0 0.0 96-97 0.5249999999999999 0.0 0.0 0.0 0.0 98-99 0.7125 0.0 0.0 0.0 0.0 100-101 0.8375 0.0 0.0 0.0 0.0 102-103 0.9625 0.0 0.0 0.0 0.0 104-105 1.05 0.0 0.0 0.0 0.0 106-107 1.175 0.0 0.0 0.0 0.0 108-109 1.275 0.0 0.0 0.0 0.0 110-111 1.4375 0.0 0.0 0.0 0.0 112-113 1.7 0.0 0.0 0.0 0.0 114-115 2.05 0.0 0.0 0.0 0.0 116-117 2.3375 0.0 0.0 0.0 0.0 118-119 2.6 0.0 0.0 0.0 0.0 120-121 3.0250000000000004 0.0 0.0 0.0 0.0 122-123 3.3625 0.0 0.0 0.0 0.0 124-125 3.6125 0.0 0.0 0.0 0.0 126-127 3.9625 0.0 0.0 0.0 0.0 128-129 4.425 0.0 0.0 0.0 0.0 130-131 4.7875 0.0 0.0 0.0 0.0 132-133 5.25 0.0 0.0 0.0 0.0 134-135 5.7375 0.0 0.0 0.0 0.0 136-137 6.1875 0.0 0.0 0.0 0.0 138-139 6.8 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTCGTAT 10 0.006830828 145.0 145 CTATCCT 10 0.006830828 145.0 6 >>END_MODULE SRR13695475 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695475_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.10525 37.0 37.0 37.0 37.0 37.0 2 35.813 37.0 37.0 37.0 37.0 37.0 3 36.074 37.0 37.0 37.0 37.0 37.0 4 35.928 37.0 37.0 37.0 37.0 37.0 5 36.183 37.0 37.0 37.0 37.0 37.0 6 36.163 37.0 37.0 37.0 37.0 37.0 7 36.032 37.0 37.0 37.0 37.0 37.0 8 36.0765 37.0 37.0 37.0 37.0 37.0 9 36.1555 37.0 37.0 37.0 37.0 37.0 10-14 36.15840000000001 37.0 37.0 37.0 37.0 37.0 15-19 36.080200000000005 37.0 37.0 37.0 37.0 37.0 20-24 36.097049999999996 37.0 37.0 37.0 37.0 37.0 25-29 36.08265 37.0 37.0 37.0 37.0 37.0 30-34 36.02975 37.0 37.0 37.0 37.0 37.0 35-39 35.97855 37.0 37.0 37.0 37.0 37.0 40-44 35.995549999999994 37.0 37.0 37.0 37.0 37.0 45-49 36.0047 37.0 37.0 37.0 37.0 37.0 50-54 35.93275 37.0 37.0 37.0 37.0 37.0 55-59 35.924549999999996 37.0 37.0 37.0 37.0 37.0 60-64 35.878150000000005 37.0 37.0 37.0 37.0 37.0 65-69 35.833299999999994 37.0 37.0 37.0 37.0 37.0 70-74 35.844049999999996 37.0 37.0 37.0 37.0 37.0 75-79 35.8298 37.0 37.0 37.0 37.0 37.0 80-84 35.7529 37.0 37.0 37.0 37.0 37.0 85-89 35.63484999999999 37.0 37.0 37.0 37.0 37.0 90-94 35.67289999999999 37.0 37.0 37.0 37.0 37.0 95-99 35.65045 37.0 37.0 37.0 37.0 37.0 100-104 35.654450000000004 37.0 37.0 37.0 37.0 37.0 105-109 35.60755 37.0 37.0 37.0 37.0 37.0 110-114 35.562149999999995 37.0 37.0 37.0 37.0 37.0 115-119 35.55505 37.0 37.0 37.0 37.0 37.0 120-124 35.34155 37.0 37.0 37.0 34.6 37.0 125-129 35.5073 37.0 37.0 37.0 34.6 37.0 130-134 35.38815 37.0 37.0 37.0 34.6 37.0 135-139 35.36605 37.0 37.0 37.0 32.2 37.0 140-144 35.27345 37.0 37.0 37.0 34.6 37.0 145-149 35.165350000000004 37.0 37.0 37.0 27.4 37.0 150-151 35.009249999999994 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 13 2.0 14 0.0 15 0.0 16 1.0 17 1.0 18 0.0 19 0.0 20 2.0 21 0.0 22 6.0 23 2.0 24 2.0 25 9.0 26 9.0 27 24.0 28 22.0 29 29.0 30 40.0 31 42.0 32 65.0 33 115.0 34 256.0 35 666.0 36 2543.0 37 164.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.509396141317964 23.12703583061889 9.596592332748685 29.766975695314457 2 28.799999999999997 25.624999999999996 31.8 13.775 3 19.375 29.45 30.85 20.325 4 24.075 34.875 22.2 18.85 5 25.474999999999998 38.475 19.575 16.475 6 19.375 43.4 20.200000000000003 17.025000000000002 7 19.900000000000002 22.15 39.65 18.3 8 21.425 26.450000000000003 27.750000000000004 24.375 9 21.5 25.374999999999996 29.799999999999997 23.325000000000003 10-14 22.595000000000002 29.62 27.365000000000002 20.419999999999998 15-19 22.75 28.825 27.560000000000002 20.865000000000002 20-24 22.571128556427823 28.55642782139107 27.85639281964098 21.016050802540125 25-29 22.48062015503876 29.002250562640658 27.771942985746435 20.745186296574143 30-34 22.393359003850577 28.194229134370158 28.479271890783618 20.933139970995647 35-39 22.89114455722786 28.54142707135357 28.31641582079104 20.251012550627532 40-44 21.738260739110867 27.83417512626894 28.5042756413462 21.92328849327399 45-49 22.252225222522252 28.047804780478046 28.962896289628965 20.737073707370737 50-54 22.051102555127756 28.33641682084104 28.42642132106605 21.18605930296515 55-59 21.710427606901725 28.502125531382845 28.927231807951987 20.86021505376344 60-64 21.706085304265212 28.751437571878597 28.136406820341016 21.406070303515175 65-69 22.58 27.98 28.775000000000002 20.665 70-74 23.190797699424856 28.067016754188543 28.132033008252062 20.610152538134532 75-79 22.849569913982798 27.880576115223043 27.820564112822566 21.449289857971593 80-84 22.62 27.725 28.875 20.78 85-89 23.625906476619154 28.387096774193548 28.042010502625658 19.94498624656164 90-94 23.54235423542354 28.81288128812881 27.61776177617762 20.027002700270028 95-99 23.711185559277965 27.431371568578427 28.581429071453574 20.276013800690034 100-104 23.80595148787197 28.69717429357339 26.216554138534633 21.280320080020005 105-109 23.250812703175793 28.24206051512878 28.48712178044511 20.02000500125031 110-114 23.411170558527928 28.616430821541076 27.251362568128407 20.721036051802592 115-119 24.266066516629156 27.636909227306827 27.541885471367845 20.555138784696176 120-124 23.80595148787197 28.617154288572145 27.786946736684172 19.789947486871718 125-129 24.41488297659532 27.815563112622527 27.475495099019803 20.29405881176235 130-134 24.76119029757439 27.421855463865967 27.371842960740185 20.445111277819457 135-139 24.921230307576895 27.656914228557138 27.106776694173547 20.315078769692423 140-144 25.056252812640633 27.821391069553474 27.28636431821591 19.83599179958998 145-149 25.541385346336583 28.31707926981745 26.70667666916729 19.43485871467867 150-151 24.731182795698924 27.094273568392097 27.85696424106027 20.31757939484871 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.5 7 1.0 8 0.5 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.5 15 0.5 16 0.0 17 0.0 18 0.5 19 1.5 20 1.0 21 0.0 22 3.5 23 5.0 24 3.0 25 3.5 26 5.0 27 6.0 28 8.5 29 20.0 30 23.0 31 20.5 32 35.0 33 45.5 34 60.5 35 84.0 36 92.0 37 122.0 38 146.5 39 149.0 40 191.0 41 241.5 42 272.5 43 281.5 44 288.0 45 283.5 46 286.0 47 253.5 48 210.0 49 190.5 50 143.0 51 122.0 52 94.5 53 61.5 54 51.0 55 51.0 56 45.0 57 30.5 58 20.5 59 12.5 60 8.0 61 6.0 62 6.0 63 4.0 64 1.5 65 1.0 66 1.0 67 1.0 68 0.5 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22499999999999998 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.005 25-29 0.025 30-34 0.015 35-39 0.005 40-44 0.015 45-49 0.01 50-54 0.005 55-59 0.025 60-64 0.005 65-69 0.0 70-74 0.025 75-79 0.02 80-84 0.0 85-89 0.025 90-94 0.01 95-99 0.005 100-104 0.025 105-109 0.025 110-114 0.005 115-119 0.025 120-124 0.025 125-129 0.02 130-134 0.025 135-139 0.025 140-144 0.005 145-149 0.025 150-151 0.025 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.325 #Duplication Level Percentage of deduplicated Percentage of total 1 69.99628666914222 47.125 2 19.123653917564056 25.75 3 6.498329001113999 13.125 4 2.6735982176011883 7.199999999999999 5 0.9654660230226514 3.25 6 0.25993316004455996 1.05 7 0.25993316004455996 1.225 8 0.11139992573338284 0.6 9 0.11139992573338284 0.675 >10 0.0 0.0 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AATACAAGAGACCACTCTGGGCAACTGGTGCTCTAGATAACCCTTCTGTG 9 0.22499999999999998 No Hit GGAAAATGGGCTGCTGAGATTCGTGATCCCAGGAAAGGGGTACGTGTCTG 9 0.22499999999999998 No Hit AGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATC 9 0.22499999999999998 No Hit AAAACAGTAATTCAAACAGTGGAAGAAGTTGCGGAGGACTGGAGACTTGA 8 0.2 No Hit CATTAATCTTTTTTGACTTCCTCTCTTCCTAATTTATCATCATTCTCGTC 8 0.2 No Hit CTCAAGCAATGATAGCAGAAAGGTTTCACGCCAAGATATTGAACTTGTCC 8 0.2 No Hit AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 7 0.17500000000000002 No Hit TACCAATTGGGTAAAATACTAACCAATATCAAAGTGGAGAGTGGAAACCC 7 0.17500000000000002 No Hit CAGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTA 7 0.17500000000000002 No Hit TTGACATTCATGTGTATTGAATAGCTGCTGAAATTGCATACAAGTTTGAC 7 0.17500000000000002 No Hit AGTGGTTCTTTCATCGGTTGAAAGCAGGCCGCCCGATTCCAATTCCCAAC 7 0.17500000000000002 No Hit AGAGAAGGCAAAGATCCGTGTCTCTCCAGTCTCGTTTTGGCAAGACCAAC 7 0.17500000000000002 No Hit AACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATCAA 7 0.17500000000000002 No Hit TGTGGCTTTAGTTGAGTTGATGTTGTACAGACTTTGCTGCCATATGTTTC 6 0.15 No Hit AGAAATTTGAAGGATTTGGTTGAAGTACCGGCAGCTGTGCTTGGCAGTCT 6 0.15 No Hit GCAAAAGGCGGTTAGACAATGGCCCAGGATCATGGTTCCCTTCATATTGT 6 0.15 No Hit ACATCATCCGGCGGCCTGTGGTATGTTTTAGGTTACATGGAGGCCAAGAA 6 0.15 No Hit ACTTAATTCATGCAGTAGATTCTTTTTTATGTACTGTAAATCTATCACAT 6 0.15 No Hit CACAAAATAAAACCCACTTGTAAAGGCTCACTCACTGAGCTTCTCCATTA 6 0.15 No Hit GTTACAAGCTGGGCTATCTGCACTTGAAGATGCGATGAAAGCAGGATTTG 6 0.15 No Hit CTCAAGAAGAACCAACTTCAATCCTCCCGGCCTTGCTGACTCAAGAATCG 5 0.125 No Hit GAGGAATCGTACTAAGAGCTCCAATCTTTAGTTAGTAGGACTGATGATGT 5 0.125 No Hit GACAAATCCAACGCTCTCGGTGAACTTCTAAAAAGCTACAACGCCGAAAA 5 0.125 No Hit GAAAAGGATGGCTTCTTTGTGACTTCTGTAAAGGGCTAAAAACCAATGTT 5 0.125 No Hit GTTTGGTCTGCCCTCCCACCCAAAAAACCCAAAAAAATCTCTCCTTTTCT 5 0.125 No Hit CGAAAGCCATCCTCTGAAACAACATCAATATGGCTCCTAAACTTTCCTGT 5 0.125 No Hit CATTTGGATTTCTAGAAGGGCTAGAATGAACAGGTTAGAGGTCAGCAATG 5 0.125 No Hit ACTTGATGTATAATACTATGTGATGTCTCTAAAATCTGGTGAAATGAGGT 5 0.125 No Hit CGCACTGCGTGTCCCGACACCCAATGTTTCAGTTGTTGACCTTGTTGTGA 5 0.125 No Hit CTGGATGGATCTGTTGAGCCAGAAAAAAGATTTGATATTGTAAAAGCTTT 5 0.125 No Hit ATACAGTCCTCGGGGACTATGTCAAGGAAAAAGTTCCAAGTCTGAAACAT 5 0.125 No Hit CGTCAACCAAACCCTAACAGAGCGTCCAATGGCCGCCTCGTTCTCGATTT 5 0.125 No Hit CTGAGAGCCAAAGGGAGGCTGCTTTATTACTTGGACAGTTTGCAGCAACC 5 0.125 No Hit CGAAGGGTTGGCTCATCTCTCTCTAAATTCTAACAATGAAACCCTTTGCT 5 0.125 No Hit TACAAAGCGTATCAAGAGCAGGTTCTTAGTAATTGCTCAAAATTTGCTCA 5 0.125 No Hit CCACCAAGGTCACATGTCTAATCTGAACCCTGGTTTCAGTTCCACGTTAC 5 0.125 No Hit CTGAATCATTCCACTCTCTCGAAGAAGTAAGCTTTGATTTGTATAAAAGT 5 0.125 No Hit CAGCAGACCTTAATCTCATCATTCTTGGAACAACCATGGTTTCCTGGGAA 5 0.125 No Hit GTATAGTACATCTTGTTTCTCTCGATATTATCCTTACCTTTCGTTTCTGC 5 0.125 No Hit GGCAAGGTCTGTTGTAGCAGAATTAATCCTTTTATGTTCTTTCATCCTGT 5 0.125 No Hit AGTGCTTTGTCAATGGAAGGAACATGTACTGGAGAACATGGAGTAGGAAC 5 0.125 No Hit AATTAGAAAATGCCTTACAGGAGAATCATGGAGGTAGAGCCGCCGAGTCC 5 0.125 No Hit CACTCCTTTCCTCTCTCTTGCCCAAGCTGCCTTGCTTGCTGTTTCAAACT 5 0.125 No Hit AGAGAAGCTGCAGAGTGAGTAGAAGAGAGATGGAGTTCCCAGTGATTAGC 5 0.125 No Hit TATAAATGTGGAGAGATTGTTTGTGGTTAAAGACAAAATCCCTATATCGT 5 0.125 No Hit CACAGAAGAAGAACATCAATGCAACTCTGATCTCTCCCTCTGTTCTCTCT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0 0.0 0.0 0.0 0.0 54-55 0.0 0.0 0.0 0.0 0.0 56-57 0.0 0.0 0.0 0.0 0.0 58-59 0.0 0.0 0.0 0.0 0.0 60-61 0.0 0.0 0.0 0.0 0.0 62-63 0.0 0.0 0.0 0.0 0.0 64-65 0.0 0.0 0.0 0.0 0.0 66-67 0.0 0.0 0.0 0.0 0.0 68-69 0.0 0.0 0.0 0.0 0.0 70-71 0.0 0.0 0.0 0.0 0.0 72-73 0.0 0.0 0.0 0.0 0.0 74-75 0.0 0.0 0.0 0.0 0.0 76-77 0.0 0.0 0.0 0.0 0.0 78-79 0.0 0.0 0.0 0.0 0.0 80-81 0.0 0.0 0.0 0.0 0.0 82-83 0.0625 0.0 0.0 0.0 0.0 84-85 0.075 0.0 0.0 0.0 0.0 86-87 0.1 0.0 0.0 0.0 0.0 88-89 0.175 0.0 0.0 0.0 0.0 90-91 0.2625 0.0 0.0 0.0 0.0 92-93 0.325 0.0 0.0 0.0 0.0 94-95 0.42500000000000004 0.0 0.0 0.0 0.0 96-97 0.55 0.0 0.0 0.0 0.0 98-99 0.7375 0.0 0.0 0.0 0.0 100-101 0.8625 0.0 0.0 0.0 0.0 102-103 0.9874999999999999 0.0 0.0 0.0 0.0 104-105 1.075 0.0 0.0 0.0 0.0 106-107 1.175 0.0 0.0 0.0 0.0 108-109 1.275 0.0 0.0 0.0 0.0 110-111 1.4375 0.0 0.0 0.0 0.0 112-113 1.7 0.0 0.0 0.0 0.0 114-115 2.075 0.0 0.0 0.0 0.0 116-117 2.3625 0.0 0.0 0.0 0.0 118-119 2.6125 0.0 0.0 0.0 0.0 120-121 3.0250000000000004 0.0 0.0 0.0 0.0 122-123 3.375 0.0 0.0 0.0 0.0 124-125 3.65 0.0 0.0 0.0 0.0 126-127 4.0375 0.0 0.0 0.0 0.0 128-129 4.525 0.0 0.0 0.0 0.0 130-131 4.9125 0.0 0.0 0.0 0.0 132-133 5.4 0.0 0.0 0.0 0.0 134-135 5.8875 0.0 0.0 0.0 0.0 136-137 6.3375 0.0 0.0 0.0 0.0 138-139 6.95 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATTCAT 10 0.006830828 145.0 5 ACATTCA 10 0.006830828 145.0 4 >>END_MODULE Read 940600 spots for SRR13695475.sra Written 940600 spots for SRR13695475.sra Read 940600 spots for SRR13695475.sra Written 940600 spots for SRR13695475.sra Read 940600 spots for SRR13695475.sra Written 940600 spots for SRR13695475.sra Read 940600 spots for SRR13695475.sra Written 940600 spots for SRR13695475.sra Read 940600 spots for SRR13695475.sra Written 940600 spots for SRR13695475.sra Read 940600 spots for SRR13695475.sra Written 940600 spots for SRR13695475.sra Read 940600 spots for SRR13695475.sra Written 940600 spots for SRR13695475.sra Read 940600 spots for SRR13695475.sra Written 940600 spots for SRR13695475.sra Read 940600 spots for SRR13695475.sra Written 940600 spots for SRR13695475.sra Read 940600 spots for SRR13695475.sra Written 940600 spots for SRR13695475.sra Read 940600 spots for SRR13695475.sra Written 940600 spots for SRR13695475.sra Read 940600 spots for SRR13695475.sra Written 940600 spots for SRR13695475.sra Read 940600 spots for SRR13695475.sra Written 940600 spots for SRR13695475.sra Read 940608 spots for SRR13695475.sra Written 940608 spots for SRR13695475.sra Read 940600 spots for SRR13695475.sra Written 940600 spots for SRR13695475.sra Read 940600 spots for SRR13695475.sra Written 940600 spots for SRR13695475.sra Read 940600 spots for SRR13695475.sra Written 940600 spots for SRR13695475.sra Read 940600 spots for SRR13695475.sra Written 940600 spots for SRR13695475.sra Read 940600 spots for SRR13695475.sra Written 940600 spots for SRR13695475.sra Read 940600 spots for SRR13695475.sra Written 940600 spots for SRR13695475.sra SRR ids: ['SRR13695475.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_utp6ehnn SRR13695475.sra spots: 18812008 blocks: [[1, 940600], [940601, 1881200], [1881201, 2821800], [2821801, 3762400], [3762401, 4703000], [4703001, 5643600], [5643601, 6584200], [6584201, 7524800], [7524801, 8465400], [8465401, 9406000], [9406001, 10346600], [10346601, 11287200], [11287201, 12227800], [12227801, 13168400], [13168401, 14109000], [14109001, 15049600], [15049601, 15990200], [15990201, 16930800], [16930801, 17871400], [17871401, 18812008]] SRR13695475 file size 6371442 SRR13695475 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695475 SRR13695475_1.fastq SRR13695475_2.fastq Input file: SRR13695475_1.fastq Paired file: SRR13695475_2.fastq trimmed: SRR13695475-trimmed-pair1.fastq, SRR13695475-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 05:55:18 2025 >> started Wed Feb 12 05:55:38 2025 >> done (19.810s) 18812008 read pairs processed; of these: 174 ( 0.00%) short read pairs filtered out after trimming by size control 340 ( 0.00%) empty read pairs filtered out after trimming by size control 18811494 (100.00%) read pairs available; of these: 1848047 ( 9.82%) trimmed read pairs available after processing 16963447 (90.18%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 5 0.00% 19 7 0.00% 20 4 0.00% 21 8 0.00% 22 5 0.00% 23 5 0.00% 24 4 0.00% 25 2 0.00% 26 9 0.00% 27 2 0.00% 28 4 0.00% 29 3 0.00% 30 2 0.00% 31 1 0.00% 32 10 0.00% 33 4 0.00% 34 7 0.00% 35 6 0.00% 36 6 0.00% 37 3 0.00% 38 13 0.00% 39 7 0.00% 40 13 0.00% 41 9 0.00% 42 20 0.00% 43 16 0.00% 44 8 0.00% 45 22 0.00% 46 6 0.00% 47 21 0.00% 48 22 0.00% 49 14 0.00% 50 28 0.00% 51 27 0.00% 52 35 0.00% 53 37 0.00% 54 40 0.00% 55 62 0.00% 56 69 0.00% 57 77 0.00% 58 67 0.00% 59 104 0.00% 60 99 0.00% 61 163 0.00% 62 167 0.00% 63 187 0.00% 64 178 0.00% 65 228 0.00% 66 280 0.00% 67 306 0.00% 68 358 0.00% 69 439 0.00% 70 505 0.00% 71 660 0.00% 72 738 0.00% 73 761 0.00% 74 974 0.01% 75 1008 0.01% 76 1313 0.01% 77 1223 0.01% 78 1560 0.01% 79 1620 0.01% 80 1961 0.01% 81 2174 0.01% 82 2670 0.01% 83 2854 0.02% 84 3351 0.02% 85 3663 0.02% 86 4070 0.02% 87 4333 0.02% 88 4649 0.02% 89 5052 0.03% 90 5609 0.03% 91 6151 0.03% 92 6498 0.03% 93 7427 0.04% 94 7950 0.04% 95 8728 0.05% 96 9502 0.05% 97 10131 0.05% 98 10466 0.06% 99 11376 0.06% 100 12132 0.06% 101 12883 0.07% 102 13554 0.07% 103 14510 0.08% 104 15242 0.08% 105 15919 0.08% 106 17130 0.09% 107 17637 0.09% 108 17968 0.10% 109 19186 0.10% 110 19770 0.11% 111 20468 0.11% 112 21265 0.11% 113 22718 0.12% 114 23739 0.13% 115 24221 0.13% 116 25696 0.14% 117 26680 0.14% 118 27461 0.15% 119 27569 0.15% 120 29120 0.15% 121 30343 0.16% 122 31289 0.17% 123 31731 0.17% 124 32870 0.17% 125 34224 0.18% 126 35136 0.19% 127 35833 0.19% 128 36239 0.19% 129 36901 0.20% 130 37827 0.20% 131 38341 0.20% 132 39974 0.21% 133 40702 0.22% 134 40771 0.22% 135 42625 0.23% 136 43747 0.23% 137 44945 0.24% 138 45483 0.24% 139 46310 0.25% 140 46737 0.25% 141 48292 0.26% 142 48161 0.26% 143 48882 0.26% 144 51002 0.27% 145 50955 0.27% 146 52335 0.28% 147 52869 0.28% 148 55153 0.29% 149 54968 0.29% 150 56338 0.30% 151 16963447 90.18% 18811494 reads passed initial QC criterion=sequence-density sequence-density=0.28 sequence-density-rank=1 fanout-score=2.27 fanout-score-rank=32 prefix-density=0.29 prefix-fanout=2.2 sequence=TGAGATGCCTCAGTGCATCCAAACATGGGTAG criterion=fanout-score sequence-density=0.01 sequence-density-rank=36 fanout-score=467.18 fanout-score-rank=1 prefix-density=0.24 prefix-fanout=17.9 sequence=TGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAGGTGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGAGTATGAATGTGTTCTCTGTG criterion=sequence-density sequence-density=0.41 sequence-density-rank=1 fanout-score=2.02 fanout-score-rank=33 prefix-density=0.41 prefix-fanout=2.0 sequence=TGTAAGAGATGGCTTCCTC criterion=fanout-score sequence-density=0.19 sequence-density-rank=7 fanout-score=31.42 fanout-score-rank=1 prefix-density=0.45 prefix-fanout=13.0 sequence=AAGAAAAGAAAA SRR13695475 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 05:56:17 Started mapping on | Feb 12 05:56:17 Finished on | Feb 12 05:58:30 Mapping speed, Million of reads per hour | 509.18 Number of input reads | 18811494 Average input read length | 297 UNIQUE READS: Uniquely mapped reads number | 17502755 Uniquely mapped reads % | 93.04% Average mapped length | 295.67 Number of splices: Total | 17420743 Number of splices: Annotated (sjdb) | 16978004 Number of splices: GT/AG | 17061912 Number of splices: GC/AG | 261687 Number of splices: AT/AC | 10870 Number of splices: Non-canonical | 86274 Mismatch rate per base, % | 0.41% Deletion rate per base | 0.03% Deletion average length | 3.00 Insertion rate per base | 0.02% Insertion average length | 2.48 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 459411 % of reads mapped to multiple loci | 2.44% Number of reads mapped to too many loci | 96413 % of reads mapped to too many loci | 0.51% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 3.87% % of reads unmapped: other | 0.14% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 849551 849551 849551 N_multimapping 459411 459411 459411 N_noFeature 729062 17102576 936815 N_ambiguous 323141 2130 129201 UnstrandedReadsAssigned:16450552 PositiveStrandReadsAssigned:398049 NegativeStrandReadsAssigned:16436739 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695475 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695475-trimmed-pair1.fastq SRR13695475-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 18,811,494 reads, 16,407,227 reads pseudoaligned [quant] estimated average fragment length: 261.311 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,113 rounds 52401 SRR13695475.ke.tsv 34699 SRR13695475.se.tsv 87100 total ==> SRR13695475.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1757.69 774 23.2978 Potri.005G024800.1.v4.1 1035 774.689 374 25.5423 Potri.004G059700.1.v4.1 961 700.921 1 0.0754827 Potri.007G009000.2.v4.1 1416 1155.69 0 0 Potri.003G141000.2.v4.1 2943 2682.69 1066 21.0234 Potri.016G087400.1.v4.1 270 82.8345 945 603.582 Potri.015G069301.1.v4.1 564 317.102 0 0 Potri.010G195200.1.v4.1 1773 1512.69 228 7.97446 Potri.012G127500.1.v4.1 977 716.827 72 5.31415 ==> SRR13695475.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 162 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 211 Potri.001G212900.v4.1 37 Potri.001G182400.v4.1 4 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 8 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 3 SRR13695475 completed mapping pipeline successfully