Starting /dee2/code/volunteer_pipeline.sh SRR13695476 current disk space = 3049670856704 free memory = 1579214236 SRR13695476 SRAfilesize 26b0bb42410f6a950f1f1c617dcdf224 SRR13695476.sra SRR13695476.sra file validated SRR13695476 is paired end SRR13695476 is conventional basespace SRR13695476 read1 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695476_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.5465 37.0 37.0 37.0 37.0 37.0 2 36.2415 37.0 37.0 37.0 37.0 37.0 3 36.5095 37.0 37.0 37.0 37.0 37.0 4 36.539 37.0 37.0 37.0 37.0 37.0 5 36.6185 37.0 37.0 37.0 37.0 37.0 6 36.5275 37.0 37.0 37.0 37.0 37.0 7 36.5205 37.0 37.0 37.0 37.0 37.0 8 36.5 37.0 37.0 37.0 37.0 37.0 9 36.54 37.0 37.0 37.0 37.0 37.0 10-14 36.5891 37.0 37.0 37.0 37.0 37.0 15-19 36.5139 37.0 37.0 37.0 37.0 37.0 20-24 36.4941 37.0 37.0 37.0 37.0 37.0 25-29 36.40939999999999 37.0 37.0 37.0 37.0 37.0 30-34 36.4007 37.0 37.0 37.0 37.0 37.0 35-39 36.39 37.0 37.0 37.0 37.0 37.0 40-44 36.4101 37.0 37.0 37.0 37.0 37.0 45-49 36.29600000000001 37.0 37.0 37.0 37.0 37.0 50-54 36.330799999999996 37.0 37.0 37.0 37.0 37.0 55-59 36.2862 37.0 37.0 37.0 37.0 37.0 60-64 36.2398 37.0 37.0 37.0 37.0 37.0 65-69 36.2512 37.0 37.0 37.0 37.0 37.0 70-74 36.2701 37.0 37.0 37.0 37.0 37.0 75-79 36.1862 37.0 37.0 37.0 37.0 37.0 80-84 36.1252 37.0 37.0 37.0 37.0 37.0 85-89 36.1069 37.0 37.0 37.0 37.0 37.0 90-94 36.0655 37.0 37.0 37.0 37.0 37.0 95-99 36.0931 37.0 37.0 37.0 37.0 37.0 100-104 36.1066 37.0 37.0 37.0 37.0 37.0 105-109 36.00840000000001 37.0 37.0 37.0 37.0 37.0 110-114 36.0375 37.0 37.0 37.0 37.0 37.0 115-119 36.03240000000001 37.0 37.0 37.0 37.0 37.0 120-124 35.9101 37.0 37.0 37.0 37.0 37.0 125-129 35.9205 37.0 37.0 37.0 37.0 37.0 130-134 35.9206 37.0 37.0 37.0 37.0 37.0 135-139 35.7825 37.0 37.0 37.0 37.0 37.0 140-144 35.740899999999996 37.0 37.0 37.0 37.0 37.0 145-149 35.6262 37.0 37.0 37.0 37.0 37.0 150-151 35.47125 37.0 37.0 37.0 31.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 20 1.0 21 0.0 22 0.0 23 2.0 24 2.0 25 7.0 26 10.0 27 10.0 28 19.0 29 30.0 30 23.0 31 37.0 32 49.0 33 67.0 34 138.0 35 314.0 36 2929.0 37 362.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.1 13.700000000000001 5.6000000000000005 37.6 2 20.150753768844222 13.165829145728644 37.58793969849246 29.095477386934675 3 17.299999999999997 17.7 26.400000000000002 38.6 4 21.15 25.900000000000002 25.0 27.950000000000003 5 24.325 29.45 24.125 22.1 6 22.025 33.575 23.275000000000002 21.125 7 14.625 29.549999999999997 39.475 16.35 8 17.150000000000002 27.900000000000002 32.125 22.825 9 16.7 23.9 35.099999999999994 24.3 10-14 19.34 30.395 27.810000000000002 22.455 15-19 20.04 28.405 27.77 23.785 20-24 19.775000000000002 28.325 28.794999999999998 23.105 25-29 19.775000000000002 28.435 28.335 23.455000000000002 30-34 19.615 28.79 28.134999999999998 23.46 35-39 20.1 29.044999999999998 27.500000000000004 23.355 40-44 20.385 28.804999999999996 27.560000000000002 23.25 45-49 20.27 28.73 27.794999999999998 23.205000000000002 50-54 20.11 28.52 28.435 22.935 55-59 20.49 27.860000000000003 28.38 23.27 60-64 19.82 28.01 28.43 23.74 65-69 20.055 28.255000000000003 27.77 23.919999999999998 70-74 19.869999999999997 29.17 27.334999999999997 23.625 75-79 19.675 27.839999999999996 28.355000000000004 24.13 80-84 20.39 28.794999999999998 28.22 22.595000000000002 85-89 20.41 28.705000000000002 27.650000000000002 23.235 90-94 20.575 28.02 28.055000000000003 23.35 95-99 19.814999999999998 28.37 27.975 23.84 100-104 19.68 29.160000000000004 27.389999999999997 23.77 105-109 20.735 28.875 27.465 22.925 110-114 20.265 27.6 27.83 24.305 115-119 20.97 27.88 27.694999999999997 23.455000000000002 120-124 20.76 28.449999999999996 27.215 23.575 125-129 21.02 27.915 27.395000000000003 23.669999999999998 130-134 21.25 28.285 27.155 23.31 135-139 20.635 28.625 26.775 23.965 140-144 21.05 28.134999999999998 26.66 24.154999999999998 145-149 21.15 28.144999999999996 27.205000000000002 23.5 150-151 21.7 27.474999999999998 26.2875 24.5375 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.5 5 0.5 6 0.5 7 0.5 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.5 17 1.0 18 1.0 19 0.5 20 1.0 21 1.0 22 1.0 23 2.0 24 1.5 25 2.0 26 3.0 27 8.0 28 14.0 29 14.0 30 17.5 31 21.5 32 29.0 33 46.5 34 62.0 35 75.5 36 116.0 37 141.0 38 144.5 39 172.0 40 207.0 41 235.5 42 244.0 43 237.0 44 239.5 45 250.5 46 248.5 47 231.0 48 195.5 49 179.5 50 165.5 51 136.5 52 114.5 53 100.0 54 90.0 55 69.0 56 59.0 57 43.5 58 21.5 59 17.5 60 12.5 61 11.0 62 9.5 63 2.0 64 0.5 65 0.5 66 0.0 67 0.5 68 0.5 69 0.0 70 0.0 71 0.0 72 0.0 73 0.0 74 0.0 75 0.0 76 0.0 77 0.0 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.5 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.0 25-29 0.0 30-34 0.0 35-39 0.0 40-44 0.0 45-49 0.0 50-54 0.0 55-59 0.0 60-64 0.0 65-69 0.0 70-74 0.0 75-79 0.0 80-84 0.0 85-89 0.0 90-94 0.0 95-99 0.0 100-104 0.0 105-109 0.0 110-114 0.0 115-119 0.0 120-124 0.0 125-129 0.0 130-134 0.0 135-139 0.0 140-144 0.0 145-149 0.0 150-151 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.925 #Duplication Level Percentage of deduplicated Percentage of total 1 74.24400417101147 53.400000000000006 2 17.205422314911367 24.75 3 5.665623913799096 12.225 4 1.70316301703163 4.9 5 0.7994438651372958 2.875 6 0.2780674313521029 1.2 7 0.03475842891901286 0.17500000000000002 8 0.03475842891901286 0.2 9 0.0 0.0 >10 0.03475842891901286 0.27499999999999997 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATCAGTAGCTCCGTGGAGTAGGCAAGTTGCCGAACCACGTAAATATGTGT 11 0.27499999999999997 No Hit GCCGCTGCCAAAACCATCATCATCACCAGTTCTCCCATATGCTTTACCAC 8 0.2 No Hit GAGATGCATTACTCCTTGTTTCTAGAAGTGCGGTCTTTTCTCGGCCCACC 7 0.17500000000000002 No Hit ACTGGCTTTAGAAGGTGGAACCATTCCATCCAAAGTGGGTACCCTGAGAG 6 0.15 No Hit TGCTCTTCTTTTTTTAACTATGTACTTTGTTGAAAACGGGGTTTTAAGCC 6 0.15 No Hit GGAGAGGCTTGTCTGTGGGTCTCTTGGGCTCACTGATCTGGTCAAGAGCA 6 0.15 No Hit ACTCATCAATGTTGAAAGCTTGCAACTCAGACCAACACAGTTCTCTTTTT 6 0.15 No Hit CTTCTCAGAACGCTTTGTATCTCTTCCCACCCGAGGATCATCAGAGCCAG 6 0.15 No Hit GCCATGAGGCAAGCCTTCAAGGCACCTAAACGTCAGAAACGGATGATGCA 6 0.15 No Hit GCTCCAAAAATTTTACCGAACAGGGCAGGAAGAAATAAAGAGGCAGTAAA 6 0.15 No Hit CAATCGTTGAGAACGCCCAGCAACTCCCACAGCCTCCCTGATCTTTGACC 6 0.15 No Hit GGCATGTTTGATATCGTAAGCATCAGAATCATCAAGCTTCAGCTTAACTA 5 0.125 No Hit GGACTGTCACAAGAAGGGTTCGGTATGCCTGGCGGTTAGCCTCAGTGTTC 5 0.125 No Hit GCTTCATATAGTCATCAAATACAAATTTCGGGTACAGATGTTTCTTCTCC 5 0.125 No Hit ATCTCATAAAAGAGCAGGGTAAGTTCTTCTGCACAGAGAGAGAAAGACAC 5 0.125 No Hit GGGCCCATCAAAGAGAGACCGAAATCAGACACCTTGGCAGTCCACGTGGA 5 0.125 No Hit GGATCACTAACCGGACCTTACTTAGCCCCTCACATCCATTCCAGATCTAC 5 0.125 No Hit CTCGCTTCAACCGCTTCAATCGTAAGATGATGCATTGTCCCCGCAACCAC 5 0.125 No Hit GTCAAGAGGGGCAGATAGATCTAGATCTTGAAGGCAAAATAATGACCAGA 5 0.125 No Hit GTCAGGTTCCACTTCTATCGGAGCTCCGGCCGGACACGGACCGGGATCCT 5 0.125 No Hit GCGAATTCTAAAAGTTACTTTTGTGATGTTTAGTCCCCCAGGGAAGAACC 5 0.125 No Hit CAGCCACTCCATATCCAGTTAGAGGCTTTCCAAGTTCGAAGGAGGAAAAA 5 0.125 No Hit CTAGCAGGTAACCAAGGGGGAGCCCAAAAACATAATAACAACCAATGTTG 5 0.125 No Hit GCTTGGACTTTGACCTGAGCTCCATCTCGATCAGCCTTTGGAGTGCCTCC 5 0.125 No Hit AGTGTAGACCAGGACAAAAGTCCCAATAATCTCAGCGCCCAATCCAGTGC 5 0.125 No Hit GACTTCATCACCAGCAAATATAAACTCAATTCCAGTCCTGGCAAGCTCAA 5 0.125 No Hit CCAACTTCAGAAATCCCATTTTCTTCATGCTTGTCTTCTGTATCAGTAAT 5 0.125 No Hit GTTTCATCAATGGCACTCTCTCACAGCCAATAACTTCAACAACTTCCCTA 5 0.125 No Hit AGCCTTTCTGGTGACCGGGAAAGCTGAGGTAGACTTGAGGCCGTTGAATG 5 0.125 No Hit GTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACC 5 0.125 No Hit CACCAACTCGCCAAACCATACTTGTTTGCAGCTAGCTGTACAGAATTTGA 5 0.125 No Hit GTATAACTTATTGAATCTCCAACGTTAAGTTCGAGGGCAACAAAGAAAGA 5 0.125 No Hit GGAGTGATGAACACCATTCAGGCGAGTAACTCCATGTTAGTGGGTTCATG 5 0.125 No Hit CGCCACCAATCTCTGTGCCACCGCAATATTCAATAATAGGTTTGTACTGA 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0125 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.037500000000000006 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.16249999999999998 0.0 0.0 0.0 0.0 78-79 0.175 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.25 0.0 0.0 0.0 0.0 84-85 0.3 0.0 0.0 0.0 0.0 86-87 0.3625 0.0 0.0 0.0 0.0 88-89 0.4625 0.0 0.0 0.0 0.0 90-91 0.5375 0.0 0.0 0.0 0.0 92-93 0.675 0.0 0.0 0.0 0.0 94-95 0.7375 0.0 0.0 0.0 0.0 96-97 0.8 0.0 0.0 0.0 0.0 98-99 0.9125 0.0 0.0 0.0 0.0 100-101 1.1625 0.0 0.0 0.0 0.0 102-103 1.3375 0.0 0.0 0.0 0.0 104-105 1.65 0.0 0.0 0.0 0.0 106-107 1.95 0.0 0.0 0.0 0.0 108-109 2.0875 0.0 0.0 0.0 0.0 110-111 2.3 0.0 0.0 0.0 0.0 112-113 2.8375 0.0 0.0 0.0 0.0 114-115 3.3 0.0 0.0 0.0 0.0 116-117 3.75 0.0 0.0 0.0 0.0 118-119 4.199999999999999 0.0 0.0 0.0 0.0 120-121 4.5875 0.0 0.0 0.0 0.0 122-123 5.2125 0.0 0.0 0.0 0.0 124-125 5.725 0.0 0.0 0.0 0.0 126-127 6.225 0.0 0.0 0.0 0.0 128-129 6.9375 0.0 0.0 0.0 0.0 130-131 7.475 0.0 0.0 0.0 0.0 132-133 8.1875 0.0 0.0 0.0 0.0 134-135 8.7375 0.0 0.0 0.0 0.0 136-137 9.287500000000001 0.0 0.0 0.0 0.0 138-139 9.7375 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content pass >>END_MODULE SRR13695476 read2 length is 151 nt ##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR13695476_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000 Sequences flagged as poor quality 0 Sequence length 151 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 36.28025 37.0 37.0 37.0 37.0 37.0 2 36.2165 37.0 37.0 37.0 37.0 37.0 3 36.2195 37.0 37.0 37.0 37.0 37.0 4 36.296 37.0 37.0 37.0 37.0 37.0 5 36.379 37.0 37.0 37.0 37.0 37.0 6 36.2495 37.0 37.0 37.0 37.0 37.0 7 36.3755 37.0 37.0 37.0 37.0 37.0 8 36.334 37.0 37.0 37.0 37.0 37.0 9 36.279 37.0 37.0 37.0 37.0 37.0 10-14 36.2821 37.0 37.0 37.0 37.0 37.0 15-19 36.227 37.0 37.0 37.0 37.0 37.0 20-24 36.1577 37.0 37.0 37.0 37.0 37.0 25-29 36.1526 37.0 37.0 37.0 37.0 37.0 30-34 36.1143 37.0 37.0 37.0 37.0 37.0 35-39 36.0826 37.0 37.0 37.0 37.0 37.0 40-44 36.0575 37.0 37.0 37.0 37.0 37.0 45-49 36.0166 37.0 37.0 37.0 37.0 37.0 50-54 36.0218 37.0 37.0 37.0 37.0 37.0 55-59 35.9919 37.0 37.0 37.0 37.0 37.0 60-64 35.9925 37.0 37.0 37.0 37.0 37.0 65-69 35.94879999999999 37.0 37.0 37.0 37.0 37.0 70-74 35.9109 37.0 37.0 37.0 37.0 37.0 75-79 35.9354 37.0 37.0 37.0 37.0 37.0 80-84 35.950399999999995 37.0 37.0 37.0 37.0 37.0 85-89 35.8607 37.0 37.0 37.0 37.0 37.0 90-94 35.841499999999996 37.0 37.0 37.0 37.0 37.0 95-99 35.8726 37.0 37.0 37.0 37.0 37.0 100-104 35.7704 37.0 37.0 37.0 37.0 37.0 105-109 35.76299999999999 37.0 37.0 37.0 37.0 37.0 110-114 35.650999999999996 37.0 37.0 37.0 37.0 37.0 115-119 35.674899999999994 37.0 37.0 37.0 37.0 37.0 120-124 35.562 37.0 37.0 37.0 37.0 37.0 125-129 35.6557 37.0 37.0 37.0 37.0 37.0 130-134 35.45465 37.0 37.0 37.0 34.6 37.0 135-139 35.5101 37.0 37.0 37.0 37.0 37.0 140-144 35.3495 37.0 37.0 37.0 34.6 37.0 145-149 35.261300000000006 37.0 37.0 37.0 32.2 37.0 150-151 34.977125 37.0 37.0 37.0 25.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 2.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 1.0 13 6.0 14 3.0 15 0.0 16 2.0 17 2.0 18 4.0 19 3.0 20 0.0 21 4.0 22 4.0 23 6.0 24 4.0 25 8.0 26 9.0 27 7.0 28 13.0 29 18.0 30 21.0 31 35.0 32 57.0 33 106.0 34 170.0 35 536.0 36 2731.0 37 248.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 37.75995990979704 26.509646705086443 10.44850914557755 25.281884239538964 2 30.3 25.5 28.625 15.575 3 20.75 27.275 31.7 20.275000000000002 4 25.75 33.050000000000004 22.825 18.375 5 26.85 36.85 20.375 15.925 6 20.474999999999998 40.125 21.525 17.875 7 19.85 23.9 37.65 18.6 8 20.375 27.0 28.999999999999996 23.625 9 22.400000000000002 24.2 31.275 22.125 10-14 23.51 29.375 26.474999999999998 20.64 15-19 22.595000000000002 29.4 27.26 20.745 20-24 23.23661830915458 29.009504752376188 27.068534267133565 20.68534267133567 25-29 22.451225612806404 28.544272136068034 28.929464732366185 20.07503751875938 30-34 23.281640820410203 27.613806903451728 28.179089544772385 20.92546273136568 35-39 22.69134567283642 28.68934467233617 27.548774387193596 21.070535267633815 40-44 23.696848424212106 27.093546773386695 28.919459729864933 20.290145072536266 45-49 23.34167083541771 28.149074537268636 27.988994497248626 20.520260130065033 50-54 22.8064032016008 28.90945472736368 27.27863931965983 21.005502751375687 55-59 22.78139069534767 28.61930965482741 27.75887943971986 20.84042021010505 60-64 23.016508254127064 27.70385192596298 28.414207103551774 20.86543271635818 65-69 23.491745872936466 27.35367683841921 27.838919459729865 21.315657828914457 70-74 23.43671835917959 28.344172086043024 26.983491745872932 21.235617808904454 75-79 22.96648324162081 28.339169584792394 27.598799399699853 21.095547773886945 80-84 23.376688344172088 27.283641820910454 28.45422711355678 20.885442721360683 85-89 22.926463231615806 27.938969484742373 28.104052026013004 21.030515257628814 90-94 24.14207103551776 27.85892946473237 27.178589294647328 20.82041020510255 95-99 22.536268134067033 28.354177088544276 28.18409204602301 20.92546273136568 100-104 23.096548274137067 28.859429714857427 27.163581790895446 20.880440220110057 105-109 23.47673836918459 28.479239619809903 27.70385192596298 20.340170085042523 110-114 23.316658329164582 28.204102051025515 27.768884442221108 20.710355177588795 115-119 23.601800900450225 28.274137068534266 28.154077038519258 19.969984992496247 120-124 25.13256628314157 28.66433216608304 26.65332666333167 19.54977488744372 125-129 25.18759379689845 27.808904452226113 26.758379189594798 20.245122561280642 130-134 26.05954465849387 28.446334751063297 26.014510883162373 19.47960970728046 135-139 24.972486243121562 29.229614807403703 25.97798899449725 19.81990995497749 140-144 26.14807403701851 28.06903451725863 26.498249124562278 19.28464232116058 145-149 26.053026513256626 27.648824412206103 27.1935967983992 19.10455227613807 150-151 25.810083823345426 29.51332415863881 26.04779181784061 18.628800200175153 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 0.0 1 0.0 2 0.0 3 1.0 4 1.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.0 10 1.5 11 0.5 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.5 19 1.0 20 0.5 21 1.0 22 1.0 23 0.5 24 0.5 25 3.0 26 5.5 27 4.5 28 4.5 29 6.0 30 11.5 31 24.0 32 32.5 33 37.5 34 55.5 35 78.5 36 101.5 37 120.0 38 136.0 39 161.0 40 204.0 41 247.5 42 260.0 43 271.5 44 280.0 45 285.0 46 268.5 47 225.0 48 208.0 49 196.0 50 144.5 51 108.0 52 104.5 53 79.0 54 73.0 55 74.0 56 43.5 57 33.5 58 35.5 59 22.5 60 11.0 61 6.5 62 4.5 63 3.0 64 3.5 65 2.5 66 1.5 67 0.5 68 0.0 69 0.5 70 0.5 71 0.0 72 0.5 73 1.0 74 0.5 75 0.5 76 1.0 77 0.5 78 0.0 79 0.0 80 0.0 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.5 90 1.0 91 0.5 92 0.0 93 0.0 94 1.0 95 1.0 96 0.0 97 0.5 98 0.5 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.22499999999999998 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-14 0.0 15-19 0.0 20-24 0.05 25-29 0.05 30-34 0.05 35-39 0.05 40-44 0.05 45-49 0.05 50-54 0.05 55-59 0.05 60-64 0.05 65-69 0.05 70-74 0.05 75-79 0.05 80-84 0.05 85-89 0.05 90-94 0.05 95-99 0.05 100-104 0.05 105-109 0.05 110-114 0.05 115-119 0.05 120-124 0.05 125-129 0.05 130-134 0.075 135-139 0.05 140-144 0.05 145-149 0.05 150-151 0.08750000000000001 >>END_MODULE >>Sequence Length Distribution pass #Length Count 151 4000.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.85000000000001 #Duplication Level Percentage of deduplicated Percentage of total 1 74.59986082115519 53.6 2 16.562282533054976 23.799999999999997 3 6.089074460681976 13.125 4 1.5309672929714684 4.3999999999999995 5 0.7654836464857342 2.75 6 0.3479471120389701 1.5 7 0.0 0.0 8 0.03479471120389701 0.2 9 0.0 0.0 >10 0.06958942240779402 0.625 >50 0.0 0.0 >100 0.0 0.0 >500 0.0 0.0 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source AGAGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAA 14 0.35000000000000003 No Hit GTGGCTTTGTATGTGATCCTTTCTAGCCTTCTGCCCCATCAAAAATAGAA 11 0.27499999999999997 No Hit GATGGGTATAGTGGCTATGATGGTTATGGGAGTTATGGGAGTTATGGAGG 8 0.2 No Hit AGTGACTTAGGACATTACTGCAATTAGATTGAACAGTAGGTTTTGATTTT 6 0.15 No Hit AGAGAAGCTCGTTTCAAGGATCAAAATGGTTCTCCAAAACGATATCGATT 6 0.15 No Hit AGCTAACCCATTAATTCAAGATGCTAGATTTGGGGATGAGAAATTCTCCC 6 0.15 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 6 0.15 No Hit CGCTGCCATGGTTGTGATGATGATCGGCAACGCCGTAAGAGGAAGAGTAG 6 0.15 No Hit CCTGACAAGATCCCATTTGTCCCTATATCTGGTTTTGAGGGTGATAACAT 6 0.15 No Hit AGAACACATTCATACTCCAAGTCTTTTAGTTCATCCATTTAAGCTTAATC 6 0.15 No Hit ACTGGCTTTAGGAGGATCTCAGGAGCCAGCAGCTTTTGGTGAGTTGGTGT 6 0.15 No Hit AGTGGTAATTTTGTGGTGTCTCACACTGAAGAGTTGCCTGGGTTCACTCC 6 0.15 No Hit ACTGGTCATAAGAAGAGGTTGCCTAAGACAAGTGATCGTTATGCTCCGCG 6 0.15 No Hit CAGTAATTCTACCACGCCTTATATTTAAAGACGAAAAGAAGTGTCTAACA 5 0.125 No Hit TGATGCTGGAGTTTTTTCCACTAGTCTTTCCAACGAGTATAAACTTGCTT 5 0.125 No Hit GTAGATTGTATGAACATAAATATTTCCTACTACTGCAGTGAATTTGTCCT 5 0.125 No Hit ATTCCATCGACATTTCCAAGCTCTCTCTCCTCCAAGACGACGACAACAAC 5 0.125 No Hit CATATCTTATAACAACATAGAATAATCAAGGATGTCTGATCAGGAAGTGA 5 0.125 No Hit AGCAGATAAGATAGCAGACACAAATTGTTATTATAGGCTCTATCTTGCAC 5 0.125 No Hit TCTGGCTATAGCTAAGCACACAAATTAAGGCTTAAAGATATAGAGAGAAA 5 0.125 No Hit CACAAATATATAAATACGAAGCCCATGATCTCCCCTTCCTTCATCTCTCC 5 0.125 No Hit ATTCCATTGACTGAGAGTTTTCCTGGAAGTGGAGGACTGATTGCAGTAAG 5 0.125 No Hit AGAGCGTGGAGCACAGAGTGATTGCTCAAACAGACGGCACTAGGATGTCG 5 0.125 No Hit CTTCAAGGCACTAGAAATCATCGAGAGAGACTCTCTAGCCATCGCCGGTA 5 0.125 No Hit CAATTTTCCACCTGCTCACCTACAACGTGAATTTCCCAGAGGTGCACCTC 5 0.125 No Hit AATCTTGTCAGGTTATTAGGATTCTGCGAAGATAGCAATGAGCGGGTGTT 5 0.125 No Hit GCTTGGGCCTTTGGTGGCATGATCTTTGTTCTTGTTTACTGCACTGCTGG 5 0.125 No Hit TGTCACTGTATTCCAGTCTCTTGTGATAGGACTGGTTTGCATGGCTGTTG 5 0.125 No Hit AGGGAACATGAAAGAGAACGTGCACGAGTGAGAGAGAAAGAAAGACATGA 5 0.125 No Hit GTGATCCCAAGTATAGTCCGGATATGGAAAAGTGCAAACTCCACATCAGG 5 0.125 No Hit AAAGGGCACTCTTTCCCCATTCCATTAAACCCCCACTTCCCTCTCCCAAT 5 0.125 No Hit ACTATGTGTACTGGGATGGAGCCCATTTTACTGAAGCTAAAGCTTGGGCC 5 0.125 No Hit AGCTAAGTAGCCTGTAAGAGATGGCTTCCTCTGTGATTTCATCGGCGGCC 5 0.125 No Hit GTGGGAGATTTAGCTCTCAACATAATCTTAACAAAATTGGGTCCAAAAGA 5 0.125 No Hit CATCAATTCTGGAGAAAAAGTTGTCAGTACCCAAGTCACGGCTCTTCCTT 5 0.125 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 0.0 0.0 28-29 0.0 0.0 0.0 0.0 0.0 30-31 0.0 0.0 0.0 0.0 0.0 32-33 0.0 0.0 0.0 0.0 0.0 34-35 0.0 0.0 0.0 0.0 0.0 36-37 0.0 0.0 0.0 0.0 0.0 38-39 0.0 0.0 0.0 0.0 0.0 40-41 0.0 0.0 0.0 0.0 0.0 42-43 0.0 0.0 0.0 0.0 0.0 44-45 0.0 0.0 0.0 0.0 0.0 46-47 0.0 0.0 0.0 0.0 0.0 48-49 0.0 0.0 0.0 0.0 0.0 50-51 0.0 0.0 0.0 0.0 0.0 52-53 0.0125 0.0 0.0 0.0 0.0 54-55 0.025 0.0 0.0 0.0 0.0 56-57 0.025 0.0 0.0 0.0 0.0 58-59 0.025 0.0 0.0 0.0 0.0 60-61 0.025 0.0 0.0 0.0 0.0 62-63 0.025 0.0 0.0 0.0 0.0 64-65 0.025 0.0 0.0 0.0 0.0 66-67 0.037500000000000006 0.0 0.0 0.0 0.0 68-69 0.05 0.0 0.0 0.0 0.0 70-71 0.075 0.0 0.0 0.0 0.0 72-73 0.125 0.0 0.0 0.0 0.0 74-75 0.125 0.0 0.0 0.0 0.0 76-77 0.16249999999999998 0.0 0.0 0.0 0.0 78-79 0.175 0.0 0.0 0.0 0.0 80-81 0.2 0.0 0.0 0.0 0.0 82-83 0.25 0.0 0.0 0.0 0.0 84-85 0.3 0.0 0.0 0.0 0.0 86-87 0.3625 0.0 0.0 0.0 0.0 88-89 0.4625 0.0 0.0 0.0 0.0 90-91 0.5249999999999999 0.0 0.0 0.0 0.0 92-93 0.65 0.0 0.0 0.0 0.0 94-95 0.7124999999999999 0.0 0.0 0.0 0.0 96-97 0.775 0.0 0.0 0.0 0.0 98-99 0.8875 0.0 0.0 0.0 0.0 100-101 1.1375 0.0 0.0 0.0 0.0 102-103 1.3125 0.0 0.0 0.0 0.0 104-105 1.625 0.0 0.0 0.0 0.0 106-107 1.925 0.0 0.0 0.0 0.0 108-109 2.0625 0.0 0.0 0.0 0.0 110-111 2.2750000000000004 0.0 0.0 0.0 0.0 112-113 2.8125 0.0 0.0 0.0 0.0 114-115 3.2625 0.0 0.0 0.0 0.0 116-117 3.7 0.0 0.0 0.0 0.0 118-119 4.1625 0.0 0.0 0.0 0.0 120-121 4.5625 0.0 0.0 0.0 0.0 122-123 5.1875 0.0 0.0 0.0 0.0 124-125 5.7 0.0 0.0 0.0 0.0 126-127 6.1875 0.0 0.0 0.0 0.0 128-129 6.8875 0.0 0.0 0.0 0.0 130-131 7.425000000000001 0.0 0.0 0.0 0.0 132-133 8.1375 0.0 0.0 0.0 0.0 134-135 8.6875 0.0 0.0 0.0 0.0 136-137 9.2375 0.0 0.0 0.0 0.0 138-139 9.7125 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTGGTG 10 0.006830828 145.0 5 TCACACA 10 0.006830828 145.0 8 >>END_MODULE Read 852341 spots for SRR13695476.sra Written 852341 spots for SRR13695476.sra Read 852341 spots for SRR13695476.sra Written 852341 spots for SRR13695476.sra Read 852341 spots for SRR13695476.sra Written 852341 spots for SRR13695476.sra Read 852341 spots for SRR13695476.sra Written 852341 spots for SRR13695476.sra Read 852341 spots for SRR13695476.sra Written 852341 spots for SRR13695476.sra Read 852341 spots for SRR13695476.sra Written 852341 spots for SRR13695476.sra Read 852341 spots for SRR13695476.sra Written 852341 spots for SRR13695476.sra Read 852341 spots for SRR13695476.sra Written 852341 spots for SRR13695476.sra Read 852341 spots for SRR13695476.sra Written 852341 spots for SRR13695476.sra Read 852341 spots for SRR13695476.sra Written 852341 spots for SRR13695476.sra Read 852341 spots for SRR13695476.sra Written 852341 spots for SRR13695476.sra Read 852341 spots for SRR13695476.sra Written 852341 spots for SRR13695476.sra Read 852341 spots for SRR13695476.sra Written 852341 spots for SRR13695476.sra Read 852341 spots for SRR13695476.sra Written 852341 spots for SRR13695476.sra Read 852341 spots for SRR13695476.sra Written 852341 spots for SRR13695476.sra Read 852341 spots for SRR13695476.sra Written 852341 spots for SRR13695476.sra Read 852344 spots for SRR13695476.sra Written 852344 spots for SRR13695476.sra Read 852341 spots for SRR13695476.sra Written 852341 spots for SRR13695476.sra Read 852341 spots for SRR13695476.sra Written 852341 spots for SRR13695476.sra Read 852341 spots for SRR13695476.sra Written 852341 spots for SRR13695476.sra SRR ids: ['SRR13695476.sra'] extra args: ['--split-files', '--defline-qual', '+'] tempdir: /tmp/pfd_0veh9jbe SRR13695476.sra spots: 17046823 blocks: [[1, 852341], [852342, 1704682], [1704683, 2557023], [2557024, 3409364], [3409365, 4261705], [4261706, 5114046], [5114047, 5966387], [5966388, 6818728], [6818729, 7671069], [7671070, 8523410], [8523411, 9375751], [9375752, 10228092], [10228093, 11080433], [11080434, 11932774], [11932775, 12785115], [12785116, 13637456], [13637457, 14489797], [14489798, 15342138], [15342139, 16194479], [16194480, 17046823]] SRR13695476 file size 5771555 SRR13695476 completed basic pipeline successfully skewer v0.2.2 [April 4, 2016] COMMAND LINE: skewer -f sanger -l 18 -q 10 -k inf -t 20 -o SRR13695476 SRR13695476_1.fastq SRR13695476_2.fastq Input file: SRR13695476_1.fastq Paired file: SRR13695476_2.fastq trimmed: SRR13695476-trimmed-pair1.fastq, SRR13695476-trimmed-pair2.fastq Parameters used: -- 3' end adapter sequence (-x): AGATCGGAAGAGCACACGTCTGAACTCCAGTCAC -- paired 3' end adapter sequence (-y): AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTA -- maximum error ratio allowed (-r): 0.100 -- maximum indel error ratio allowed (-d): 0.030 -- end quality threshold (-q): 10 -- minimum read length allowed after trimming (-l): 18 -- file format (-f): Sanger/Illumina 1.8+ FASTQ -- number of concurrent threads (-t): 20 Wed Feb 12 05:55:03 2025 >> started Wed Feb 12 05:55:22 2025 >> done (18.510s) 17046823 read pairs processed; of these: 124 ( 0.00%) short read pairs filtered out after trimming by size control 332 ( 0.00%) empty read pairs filtered out after trimming by size control 17046367 (100.00%) read pairs available; of these: 2301070 (13.50%) trimmed read pairs available after processing 14745297 (86.50%) untrimmed read pairs available after processing Length distribution of reads after trimming: length count percentage 18 4 0.00% 19 3 0.00% 20 4 0.00% 21 1 0.00% 22 2 0.00% 23 3 0.00% 24 1 0.00% 25 2 0.00% 26 2 0.00% 27 1 0.00% 28 1 0.00% 29 4 0.00% 30 4 0.00% 31 1 0.00% 32 4 0.00% 33 8 0.00% 34 2 0.00% 35 7 0.00% 36 6 0.00% 37 1 0.00% 38 7 0.00% 39 10 0.00% 40 6 0.00% 41 6 0.00% 42 12 0.00% 43 13 0.00% 44 6 0.00% 45 15 0.00% 46 14 0.00% 47 23 0.00% 48 27 0.00% 49 32 0.00% 50 42 0.00% 51 54 0.00% 52 61 0.00% 53 66 0.00% 54 92 0.00% 55 95 0.00% 56 97 0.00% 57 113 0.00% 58 145 0.00% 59 168 0.00% 60 200 0.00% 61 265 0.00% 62 298 0.00% 63 347 0.00% 64 303 0.00% 65 429 0.00% 66 468 0.00% 67 509 0.00% 68 595 0.00% 69 811 0.00% 70 826 0.00% 71 1076 0.01% 72 1259 0.01% 73 1343 0.01% 74 1507 0.01% 75 1708 0.01% 76 2016 0.01% 77 2262 0.01% 78 2332 0.01% 79 2819 0.02% 80 3158 0.02% 81 3747 0.02% 82 4102 0.02% 83 4571 0.03% 84 5357 0.03% 85 5832 0.03% 86 6228 0.04% 87 6913 0.04% 88 7271 0.04% 89 7720 0.05% 90 8606 0.05% 91 9417 0.06% 92 10143 0.06% 93 10909 0.06% 94 11873 0.07% 95 12834 0.08% 96 13423 0.08% 97 14506 0.09% 98 15343 0.09% 99 16362 0.10% 100 16927 0.10% 101 17457 0.10% 102 18675 0.11% 103 19335 0.11% 104 21172 0.12% 105 21858 0.13% 106 23376 0.14% 107 23934 0.14% 108 24567 0.14% 109 25634 0.15% 110 26511 0.16% 111 27330 0.16% 112 28489 0.17% 113 29251 0.17% 114 30692 0.18% 115 31918 0.19% 116 32902 0.19% 117 34306 0.20% 118 35442 0.21% 119 36023 0.21% 120 37371 0.22% 121 38414 0.23% 122 38658 0.23% 123 39161 0.23% 124 40850 0.24% 125 41475 0.24% 126 43267 0.25% 127 43865 0.26% 128 44776 0.26% 129 46350 0.27% 130 47301 0.28% 131 47274 0.28% 132 47626 0.28% 133 49161 0.29% 134 49580 0.29% 135 50389 0.30% 136 51340 0.30% 137 52634 0.31% 138 53679 0.31% 139 55522 0.33% 140 55601 0.33% 141 56146 0.33% 142 56993 0.33% 143 57533 0.34% 144 59312 0.35% 145 59279 0.35% 146 59060 0.35% 147 60844 0.36% 148 62517 0.37% 149 63269 0.37% 150 65171 0.38% 151 14745297 86.50% 17046367 reads passed initial QC criterion=sequence-density sequence-density=0.55 sequence-density-rank=1 fanout-score=1.96 fanout-score-rank=31 prefix-density=0.55 prefix-fanout=1.9 sequence=TGTCGCAGGTGCTAGACATGATGATTGATTGATTAAGCTTAAATGGATGAACTAAAAGACTTGGAGTATGAATGTGTTCTCTGTGTA criterion=fanout-score sequence-density=0.03 sequence-density-rank=32 fanout-score=27.41 fanout-score-rank=1 prefix-density=0.12 prefix-fanout=7.3 sequence=AATGAAGAAGACACAAAGATAAAGACACTCACGGACACTTTTGTTTGTTCACTCCACACCCACAAGTACAGAAGAACACAGATTATTTATCAGAAATTACATTATTAATCCACTCCCAATCCCACATCAAACATGATAGTTGATGTGCTTGCTTAATGACCGCATGTGCAGGTAGTGCAAGTGCAGCCAGTACCGCAGTTGCACTTGCCATCGTTCTCAGCTGCTGGCACCTCCATGACTACGGAGGAGACATAGTTCTTCTCAGTCTCGACGATGCCAGCAGTGTAGCTGCTTCCCTTCTTGACACACTGGGTCTTGTCAGCGCAGTCGCAG criterion=sequence-density sequence-density=1.57 sequence-density-rank=1 fanout-score=2.03 fanout-score-rank=32 prefix-density=1.58 prefix-fanout=2.0 sequence=CTGCGACTGCGCTGACAAGACCCAGTGTGTCAAGAAGGGAAGCAGCTACACTGCTGGCATCGTCGAGACTGAGAAGAACTATGTCTCCTCCGTAGTCATGGAGGTGCCAGCAGCTGAGAACGATGGCAAGTGCAACTGCGGTACTGGCTGCACTTGCACTACCTGCACATGCGGTCATTAAGCAAGCACATCAACTATCATGTTTGATGTGGGATTGGGAGTGGATTAATAATGTAATTTCTGATAAATAATCTGTGTTCTTCTGTACTTGTGGGTGTGGAGTGAACAAACAAAAGTGTCCGTGAGTGTCTTTATCTTTGTGTCTTCTTCATT criterion=fanout-score sequence-density=0.01 sequence-density-rank=40 fanout-score=39.70 fanout-score-rank=1 prefix-density=0.07 prefix-fanout=3.6 sequence=GCAAAACCACATATAGAGGGTGTAATAGCTAAGTAGCCTGTAAGAGATGGCTTCCTCTGTGATTTCATCGGCGGCCGTTGCCACAGTTAACCGCACCCC SRR13695476 testing PE reads STAR mapping to Ensembl genome Started job on | Feb 12 05:56:04 Started mapping on | Feb 12 05:56:05 Finished on | Feb 12 05:58:29 Mapping speed, Million of reads per hour | 426.16 Number of input reads | 17046367 Average input read length | 295 UNIQUE READS: Uniquely mapped reads number | 15761722 Uniquely mapped reads % | 92.46% Average mapped length | 294.06 Number of splices: Total | 15410806 Number of splices: Annotated (sjdb) | 15041362 Number of splices: GT/AG | 15099296 Number of splices: GC/AG | 235816 Number of splices: AT/AC | 11020 Number of splices: Non-canonical | 64674 Mismatch rate per base, % | 0.37% Deletion rate per base | 0.03% Deletion average length | 2.83 Insertion rate per base | 0.02% Insertion average length | 2.36 MULTI-MAPPING READS: Number of reads mapped to multiple loci | 416416 % of reads mapped to multiple loci | 2.44% Number of reads mapped to too many loci | 28469 % of reads mapped to too many loci | 0.17% UNMAPPED READS: % of reads unmapped: too many mismatches | 0.00% % of reads unmapped: too short | 4.82% % of reads unmapped: other | 0.11% CHIMERIC READS: Number of chimeric reads | 0 % of chimeric reads | 0.00% N_unmapped 868420 868420 868420 N_multimapping 416416 416416 416416 N_noFeature 601676 15303129 842789 N_ambiguous 310819 1893 92027 UnstrandedReadsAssigned:14849227 PositiveStrandReadsAssigned:456700 NegativeStrandReadsAssigned:14826906 Dataset is classified negative stranded MeadianReadLen=151 20thPercentileLength=151 echo kmer=147 SRR13695476 Starting Kallisto paired end mapping to ensembl reference transcriptome [quant] fragment length distribution will be estimated from the data [index] k-mer length: 31 [index] number of targets: 52,400 [index] number of k-mers: 62,057,036 [index] number of equivalence classes: 130,681 [quant] running in paired-end mode [quant] will process pair 1: SRR13695476-trimmed-pair1.fastq SRR13695476-trimmed-pair2.fastq [quant] finding pseudoalignments for the reads ... done [quant] processed 17,046,367 reads, 14,798,078 reads pseudoaligned [quant] estimated average fragment length: 242.062 [ em] quantifying the abundances ... done [ em] the Expectation-Maximization algorithm ran for 1,099 rounds 52401 SRR13695476.ke.tsv 34699 SRR13695476.se.tsv 87100 total ==> SRR13695476.ke.tsv <== target_id length eff_length est_counts tpm Potri.005G200100.1.v4.1 2018 1776.94 669 19.4414 Potri.005G024800.1.v4.1 1035 793.938 646 42.0165 Potri.004G059700.1.v4.1 961 720.013 1 0.0717188 Potri.007G009000.2.v4.1 1416 1174.94 0 0 Potri.003G141000.2.v4.1 2943 2701.94 1158 22.1313 Potri.016G087400.1.v4.1 270 87.1262 1205 714.187 Potri.015G069301.1.v4.1 564 331.069 0 0 Potri.010G195200.1.v4.1 1773 1531.94 237 7.98879 Potri.012G127500.1.v4.1 977 735.968 42 2.94689 ==> SRR13695476.se.tsv <== Potri.001G166300.v4.1 0 Potri.001G448400.v4.1 168 Potri.001G233950.v4.1 0 Potri.001G122700.v4.1 294 Potri.001G212900.v4.1 67 Potri.001G182400.v4.1 0 Potri.001G256600.v4.1 0 Potri.001G040500.v4.1 6 Potri.001G416900.v4.1 0 Potri.001G452600.v4.1 14 SRR13695476 completed mapping pipeline successfully